Fact-checked by Grok 2 weeks ago

A-DNA

A-DNA is a right-handed double-helical form of double-stranded deoxyribonucleic acid (DNA) that adopts a compact, wide structure under low-humidity or dehydrating conditions, featuring 11 base pairs per helical turn, a helical rise of 2.55 Å per base pair, and a central axis hole approximately 9 Å in diameter.^[1] This conformation, first identified through X-ray fiber diffraction studies of DNA fibers at about 75% relative humidity, contrasts with the more elongated B-DNA form prevalent in aqueous environments. Discovered by Rosalind Franklin and Raymond Gosling in 1953 via crystallographic analysis of sodium thymonucleate fibers, A-DNA's structure was further elucidated in subsequent work showing its C3'-endo sugar puckering and tilted base pairs (approximately 20° relative to the helical axis), which result in shallow major and minor grooves compared to the deeper grooves in B-DNA. In biological contexts, A-DNA plays a role in protein-DNA interactions, such as those involving the TATA-box binding protein (TBP), where it facilitates specific recognition through an enlarged major groove. Additionally, in dormant bacterial spores like those of Bacillus subtilis, A-DNA conformation induced by small acid-soluble spore proteins (SASPs) enhances resistance to ultraviolet (UV) radiation by altering DNA's photochemical reactivity and compacting the nucleoid. These properties underscore A-DNA's importance in structural biology, though it is less common in vivo than B-DNA under physiological conditions.

History and Discovery

Early Observations

The initial experimental evidence for distinct conformations of DNA emerged from X-ray diffraction studies on oriented fibers in the pre-1950s era, where variations in diffraction patterns were observed depending on environmental conditions such as humidity levels. These studies revealed that DNA fibers exhibited different structural arrangements, with lower humidity producing more compact, ordered patterns indicative of a dehydrated form, while higher humidity yielded more extended structures. This humidity-dependent polymorphism suggested that DNA could adopt multiple conformations, though the molecular basis remained unclear at the time.^[2] A pivotal contribution came from William Astbury and Florence Bell in 1938, who conducted X-ray diffraction on sodium thymonucleate fibers and identified a "crystalline" form characterized by a strong meridional reflection at approximately 3.3 Å, observed under low-humidity conditions around 75% relative humidity. They described this compact structure as resembling a "zigzag" backbone with stacked bases, terming it an early precursor to what would later be known as the A-form, although their model lacked details on helical geometry and was not fully interpreted due to limited sample quality and resolution. This work, presented at the Cold Spring Harbor Symposium, highlighted the influence of hydration on DNA's physical properties but was hampered by impure preparations that mixed forms, preventing a complete understanding.^[3]^[2] In the early 1950s, Maurice Wilkins and his colleagues at King's College London advanced these observations through improved fiber diffraction experiments using high-purity DNA samples. By controlling humidity in a controlled environment, they distinguished a paracrystalline, hydrated form—later identified as the B-form—with a clear 3.4 Å repeat and helical indications, from the more crystalline, low-humidity A-form exhibiting a tilted base stacking. These experiments, including those with Raymond Gosling, provided sharper patterns that confirmed the polymorphism noted by Astbury and emphasized the role of water content in stabilizing different DNA structures.^[4] These foundational studies established A-DNA as a distinct, compact conformation arising under dehydrating conditions, setting the stage for subsequent refinements.

Rosalind Franklin's Contribution

Rosalind Franklin, working at King's College London, collaborated with Raymond Gosling to investigate the structure of DNA using X-ray fiber diffraction techniques on oriented fibers of sodium thymonucleate derived from calf thymus. Their efforts distinguished two major forms of DNA based on humidity conditions, with the A-form emerging as a key crystalline structure under controlled dehydration. In 1952, Franklin and Gosling produced high-resolution X-ray diffraction images of the A-form by maintaining sodium thymonucleate fibers at approximately 75% relative humidity, corresponding to a water content of about 40-45% of the dry weight. These images, distinct from the more hydrated B-form patterns obtained at over 90% relative humidity, revealed a highly ordered, crystalline lattice with strong meridional reflections at 2.56 Å spacing and layer lines indicating a pitch of about 28 Å. The patterns displayed diamond-shaped absences and intense off-meridional arcs, characteristic of a compact, dehydrated conformation. Analysis of these diffraction patterns led Franklin and Gosling to interpret the A-form as a right-handed helical structure, with approximately 11 nucleotide residues per helical turn and a translation of 2.56 Å per residue along the axis. The base pairs were inferred to be tilted relative to the helical axis, contributing to the structure's shorter and wider geometry compared to the B-form, with phosphate groups positioned on the periphery of a helix roughly 20 Å in diameter. This interpretation suggested possible hydrogen bonding between tilted bases to link the polynucleotide chains, forming a stable, rigid framework. Franklin and Gosling detailed these findings in two seminal 1953 publications: a brief account in Nature emphasizing the helical nature and humidity-dependent polymorphism, and more extensive analyses in Acta Crystallographica exploring the influence of water content and cylindrical Patterson functions to refine the structural model of the A-form as a dehydrated, compact helix.

Structural Characteristics

Helical Geometry

A-DNA forms a right-handed double helix, characterized by a helical twist that accommodates 11 base pairs per turn and a pitch of 28.2 Å.^[5] This configuration results in an axial rise of 2.56 Å per base pair, producing a compact structure along the helix axis that is shorter overall compared to other DNA conformations.^[5] The helix is notably wider, with a diameter of approximately 23 Å, reflecting the outward displacement of the sugar-phosphate backbone.^[6] A defining feature of A-DNA's geometry is the C3'-endo pucker of the deoxyribose sugar rings, which positions the base pairs away from the helical axis and promotes a more rigid, extended backbone conformation.^[5] This sugar conformation, combined with the helical parameters, creates a central hole along the axis with a diameter of about 9 Å, a structural hallmark observable in X-ray diffraction patterns.^[1] The base pairs themselves exhibit a significant tilt of approximately 20° relative to the helical axis, further contributing to the helix's distinctive squat and broad appearance.^[5]

Base Pairing and Grooves

In A-DNA, the double helix maintains the standard Watson-Crick base pairing scheme, with adenine (A) forming two hydrogen bonds with thymine (T) and guanine (G) forming three hydrogen bonds with cytosine (C). This pairing geometry ensures the bases lie nearly perpendicular to the plane defined by the glycosidic bonds, but the base pair planes themselves are tilted by approximately 20° relative to the helical axis, contributing to the overall compactness of the structure.^[7] The hydrogen bonding patterns are identical to those in other DNA conformations, preserving the specificity of genetic information storage. The tilted base pairs in A-DNA are displaced laterally from the helical axis by about 4.5 Å toward the minor groove side, creating an asymmetry that profoundly influences the accessibility of the grooves. This displacement, combined with the C3'-endo sugar pucker of the deoxyribose rings and the ~20° tilt of the bases, results in a characteristic groove architecture: a deep and narrow major groove measuring approximately 2.7 Å in width and a wide and shallow minor groove approximately 12 Å in width. The narrow major groove limits access to the edges of the bases on that side, while the expansive minor groove exposes a larger surface area, though its shallowness reduces depth-related interactions. Base stacking in A-DNA occurs with an average vertical distance of ~3.3 Å between consecutive base pairs, slightly shorter than in more hydrated forms, which supports the stable stacking interactions despite the tilted orientation. These stacking distances, along with the off-axis positioning, enhance the hydrophobic interactions between overlapping bases, stabilizing the A-form under low-humidity conditions. The overall helical tilt referenced briefly from prior geometric parameters further accentuates the groove asymmetry without altering the fundamental base pairing integrity.

Comparison to Other DNA Conformations

With B-DNA

A-DNA and B-DNA, the two predominant right-handed conformations of double-stranded DNA, exhibit distinct helical geometries that influence their overall shape and functional properties. B-DNA, the canonical form prevalent in physiological conditions, features approximately 10.5 base pairs per helical turn, a rise of 3.4 Å per base pair along the helix axis, and a uniform pitch of 34 Å per turn.^[8] These parameters result in a longer, narrower cylindrical structure with a diameter of about 20 Å.^[1] In contrast, A-DNA is shorter and wider, with 11 base pairs per turn and a smaller axial rise of 2.6 Å per base pair, leading to a more compact helix that accommodates fewer base pairs along its length for a given number of nucleotides.^[1]^[9] A key structural distinction lies in the dimensions and accessibility of their major and minor grooves. In B-DNA, the major groove is wide (approximately 12 Å) and deep (about 8.5 Å), while the minor groove is narrow (around 6 Å) but similarly deep, providing ample space for protein interactions in the major groove.^[10]^[11] Conversely, A-DNA displays an inverted groove profile: its major groove is deep and narrow, and the minor groove is wide and shallow, reducing the accessibility for large-scale binding compared to B-DNA.^[10] These groove differences arise from the tilted base pairs in A-DNA (about 20° relative to the helix axis) versus the near-perpendicular orientation in B-DNA, altering the sugar-phosphate backbone positioning.^[1] The stability of these conformations is highly dependent on environmental hydration levels. B-DNA is thermodynamically favored and stable in aqueous solutions with high relative humidity (>92%), where water molecules effectively hydrate the phosphate backbone and grooves.^[12] A-DNA, however, forms under dehydrating conditions at low relative humidity (<75% RH), such as in fiber preparations, where reduced water availability promotes a more dehydrated, compact structure.^[12]^[1] In solution, A-DNA is energetically less stable than B-DNA by approximately 0.5 kcal/mol per base pair in aqueous solution, as estimated from simulations of short DNA segments, reflecting the higher free energy cost of its dehydrated state in hydrated environments.^[13] This subtle energy difference underscores B-DNA's dominance in vivo, while A-DNA's conformation emerges under specific stress or dehydration scenarios.^[13]

With Z-DNA

Z-DNA represents a left-handed double-helical conformation of DNA, in stark contrast to the right-handed helix of A-DNA. While A-DNA features approximately 11 base pairs per turn with a rise of 2.6 Å per base pair, Z-DNA exhibits 12 base pairs per turn and a helical rise of 3.8 Å per base pair, resulting in a more elongated and narrower structure overall. The zigzag arrangement of the phosphate backbone in Z-DNA, arising from alternating syn and anti glycosidic conformations of the sugars, gives it its name and distinguishes it from the smoother backbone of A-DNA.^[14] Unlike A-DNA, which forms independently of sequence under dehydrating conditions, Z-DNA requires specific alternating purine-pyrimidine sequences, such as CG repeats, to stabilize its conformation; purines adopt the syn glycosidic bond, while pyrimidines remain anti, facilitating the left-handed twist. This sequence dependence contrasts with A-DNA's broader applicability across various sequences in low-humidity environments. Both conformations are compact relative to the more hydrated B-DNA, yet Z-DNA lacks the central hole present in A-DNA and features grooves of roughly equal depth, with a deep minor groove and an everted major groove that exposes certain guanine atoms on its convex surface.^[14]^[15] The stability of Z-DNA is promoted by high salt concentrations or negative supercoiling, which relieve torsional stress and favor the left-handed form, whereas A-DNA is induced primarily by dehydration that reduces the helical rise and tilts the base pairs. These differing triggers highlight their specialized environmental roles as non-B DNA forms, potentially involved in distinct regulatory processes within cells.^[14]^[15]

Conformational Transitions

A to B Transition

The A to B transition in DNA is a humidity-driven conformational change observed primarily in oriented DNA fibers, where A-DNA predominates at relative humidities (RH) below 75%, converting to B-DNA at RH above 92% through intermediate hydration states.^[16]^[17] This shift is reversible and cooperative, reflecting the molecule's response to water availability, with early observations documented via X-ray fiber diffraction patterns that distinctly vary with environmental moisture.^[16]^[18] At the molecular level, the transition involves a key change in deoxyribose sugar puckering from the C3'-endo conformation characteristic of A-DNA to the C2'-endo form in B-DNA, which repositions the base pairs to become more perpendicular to the helical axis, thereby straightening them and elongating the overall helix from approximately 2.55 Å per base pair rise in A-DNA to 3.4 Å in B-DNA.^[1]^[19] This pucker adjustment, coupled with alterations in glycosidic torsion angles, reduces the helix tilt and diameter, transforming the compact, wide A-form into the extended, narrow B-form.^[1] Kinetically, the process is monitored through fiber X-ray diffraction, revealing a reversible transition with a midpoint around 81% RH for typical DNA sequences, where hybrid diffraction features emerge before full conversion.^[18] The rate depends on crystallinity and hydration gradients, often completing in days under controlled conditions.^[20] Thermodynamically, B-DNA exhibits lower free energy in physiological aqueous environments due to favorable solvation of its phosphate backbone and groove structures, rendering the A to B shift spontaneous under high water activity. During this change, the major groove widens and shallows while the minor groove narrows and deepens, accommodating more water molecules in the B-form.^[18]

A/B Intermediates

A/B intermediates represent hybrid DNA conformations that blend characteristics of A-DNA and B-DNA, often arising in specific sequence contexts such as those with modified bases or high GC content. In sequences featuring methylated or brominated cytosines, such as the hexamer d(GGm⁵CGCC)₂ or d(GGBr⁵CGCC)₂, these intermediates form under crystallization conditions in aqueous solutions containing magnesium and spermine, exhibiting a composite structure where the 5'-end adopts A-DNA-like features and the 3'-end retains B-DNA-like geometry.^[21] This mixed conformation is characterized by heterogeneous sugar puckering, with C3'-endo (A-like) at the 5'-terminal nucleotides and C2'-endo (B-like) at the 3'-end, facilitating a gradual transition pathway.^[21] Heteronomous duplexes exemplify another type of A/B intermediate, where the two strands adopt distinct helical geometries—one A-like and the other B-like—despite being paired. In GC-rich fragments like d(GGGGCCCC), the purine-rich d(G)₄ strand displays A-like guanine-guanine stacking with C3'-endo puckering, while the pyrimidine-rich d(C)₄ strand maintains B-like C2'-endo puckering, resulting in an overall heteronomous structure.^[22] Such asymmetry arises from sequence-dependent preferences, particularly in purine-pyrimidine tracts, and has been observed in other modified or alternating sequences.^[23] These intermediates have been characterized in aqueous solutions using NMR spectroscopy and X-ray crystallography, revealing their structural details at resolutions of 1.3–2.0 Å. For instance, in the GC-rich dodecamer d(CATGGGCCCATG)₂, crystallography shows a stable intermediate with predominantly C3'-endo puckers but one C2'-endo at the terminus, an X-displacement of -2.9 Å, and a minor groove width of 9.5 Å, bridging A-DNA (-4 to -6 Å displacement, 10.0 Å groove) and B-DNA (0 Å displacement, 6.2 Å groove).^[24] In shorter CG-rich oligomers like d(CG)₄ variants, NMR in aqueous environments detects mixed puckering and partial A-like stacking, consistent with these hybrid forms.^[25] Due to their energetic instability, with barriers as low as 0.2 kcal/mol in G-tract regions, A/B intermediates serve primarily as kinetically trapped transient states during broader conformational shifts, rather than stable endpoints.^[24]^[21]

Biological Significance

Conditions for Formation

The formation of A-DNA is primarily triggered by dehydration, which reduces water activity to below 0.75 (corresponding to relative humidity under 75%), causing the extended B-form of DNA to collapse into the more compact A-form as observed in X-ray fiber diffraction studies.^[16] This transition occurs because low hydration levels disrupt the water spine in the minor groove of B-DNA, favoring the tilted base pairs and deeper major groove characteristic of the A-form.^[26] High salt concentrations, such as around 1 M NaCl, promote A-DNA stabilization in vitro by screening phosphate repulsions and effectively mimicking dehydration effects, thereby reducing the solvent's ability to hydrate the helix fully.^[27] Similarly, organic solvents like ethanol induce the A-form at concentrations around 80% by lowering water activity and altering solvation around the DNA backbone, as demonstrated in molecular dynamics simulations and spectroscopic analyses.^[28] Neutral pH (around 7) also supports A-DNA formation in these dehydrating environments, as extreme pH values tend to destabilize the helix or promote alternative conformations.^[27] In vitro, polyamines such as spermidine and spermine, or divalent cations like Mg²⁺, induce and stabilize the A-form at millimolar concentrations by bridging phosphate groups and promoting compaction, analogous to dehydration-induced changes.^[29] This similarity underscores the A-form's resemblance to the stable double helix of RNA, which persists under comparable low-hydration conditions.^[28]

Roles in Organisms

A-DNA plays a significant role in the genomes of viruses infecting hyperthermophilic archaea, where it provides stability in extreme environments characterized by high temperatures and low water activity. For instance, the rod-shaped virus SIRV2, which infects the archaeon Sulfolobus islandicus at temperatures around 80°C and pH 3, encapsidates its double-stranded DNA entirely in the A-form, as determined by cryo-electron microscopy at 3.8 Å resolution.^[30] This conformation, with an average base pair inclination of 25° and a helical diameter of ~24 Å, is protected by α-helices from the capsid protein, creating a solvent-inaccessible core that shields the genome from thermal denaturation and desiccation. Similarly, the filamentous virus AFV1, infecting Acidianus species in acidic hot springs, maintains its dsDNA in A-form within the virion, stabilized by heterodimeric capsid proteins that coordinate phosphate groups via positively charged residues.^[31] These adaptations highlight A-DNA's prevalence in archaeal viruses adapted to hyperthermophilic conditions, where dehydration promotes the B-to-A transition to enhance genomic resilience.^[30] In viral DNA packaging, A-DNA's compact structure facilitates efficient genome enclosure within capsids, particularly in environments favoring low hydration. The A-form helix, with a rise per base pair of approximately 2.56 Å compared to 3.38 Å in B-DNA, shortens the axial length of the genome by about 25%, allowing denser packing without compromising structural integrity. This property is exemplified in archaeal viruses like SIRV2, where the compact A-DNA fills the rod-shaped capsid with a protein-to-DNA mass ratio of 3.5:1, analogous to mechanisms in bacterial spores that protect DNA under desiccation.^[30] Although primarily documented in archaeal systems, similar compactness contributes to packaging efficiency in some bacteriophages under dehydrating conditions during assembly, reducing the effective genome length to optimize capsid filling.^[31] A-DNA also participates in DNA-protein interactions within cellular contexts prone to dehydration, such as membrane-associated or high-salt compartments, where its distinctive grooves enable specific binding by regulatory proteins. The shallow minor groove and deep, narrow major groove of A-DNA facilitate interactions with transcription factors that recognize dehydrated or sequence-induced A-like conformations in promoters.^[32] For example, in eukaryotic systems, the transcription factor ILF3 binds A-form promoter elements like that of the gdi3 gene, modulating expression during development through indirect readout of the helical geometry.^[32] In prokaryotic analogs, particularly in extremophiles, such bindings may stabilize regulatory complexes in low-water intracellular niches, preventing unwinding under stress. In dormant bacterial spores, such as those of Bacillus subtilis, A-DNA is induced by binding of small acid-soluble spore proteins (SASPs). This conformation alters DNA's photochemical reactivity, enhancing resistance to ultraviolet radiation, and compacts the nucleoid for protection during dormancy under dehydrating conditions.^[1]^[33] Recent studies have uncovered A-DNA's involvement in RNA-DNA hybrids formed during transcription, particularly in extremophilic organisms where these structures stabilize transient intermediates against harsh conditions. RNA-DNA hybrids inherently adopt an A-form helix due to the RNA strand's conformational preferences, as seen in crystal structures of RNase H complexes from thermophilic bacteria like Thermovibrio ammonificans.^[34] In archaea, such as halophiles and thermophiles, these hybrids arise as R-loops during replication-transcription conflicts, and their A-form configuration resists hydrolysis, aiding processivity in high-temperature or saline environments.^[34] Post-2020 analyses of RNase H biodiversity emphasize how this stabilization prevents genomic instability in extremophiles, with enzymes like archaeal RNase HII selectively cleaving hybrid junctions to resolve intermediates without disrupting the A-form scaffold.^[34]

References

[1]
DNA: Alternative Conformations and Biology - NCBI - NIH
A-DNA has 11 base pairs per helical turn, base pairs are tilted to about 20°, with respect to the helical axis, the grooves are not as deep as those in B-DNA, ...Introduction · B-DNA and A-DNA · Biological Significance
[2]
X-Ray Study of Thymonucleic Acid - Nature
ASTBURY, W., BELL, F. X-Ray Study of Thymonucleic Acid. Nature 141, 747–748 (1938). https://doi.org/10.1038/141747b0. Download citation. Published: 01 April ...
[3]
SOME RECENT DEVELOPMENTS IN THE X-RAY STUDY OF ...
SOME RECENT DEVELOPMENTS IN THE X-RAY STUDY OF PROTEINS AND RELATED STRUCTURES. W. T. Astbury and; Florence O. Bell ... 1938. 6: 109-121. » ExcerptFree; Full Text ...
[4]
Photograph 51, by Rosalind Franklin (1952)
Dec 30, 2019 · Maurice Wilkins, a scientist working at King´s College London, collected X-ray diffraction patterns of DNA in 1950. Wilkins and his graduate ...
[5]
https://doi.org/10.1016/0006-291X(72)90243-4
[6]
Forms of DNA - Proteopedia, life in 3D
Jul 6, 2025 · Helical Parameters of the three forms of DNA ; Diameter of helix [Å], 23, 20, 18 ; Glycosidic bond configuration dA,dT,dC dG, anti anti, anti anti
[7]
[PDF] CHAPTER 2 - Structural Biology @ Vanderbilt
DNA fragments containing TATA boxes has revealed a DNA structure that shares features of A- and B-DNA. In addition, A- and B-DNA polymorphs can coexist, as ...
[8]
https://bionumbers.hms.harvard.edu/bionumber.aspx?id=100667
[9]
DNA helix rise per base pair - Generic - BNID 100667 - BioNumbers
DNA helix rise per base pair ; Value, 0.34 nm ; Organism, Generic ; Reference, Arnott S, Chandrasekaran R, Birdsall DL, Leslie AG, Ratliff RL. Left-handed DNA ...Missing: standard diameter
[10]
[PDF] DNA Structure: A-, B- and Z-DNA Helix Families
A-form DNA was first identified from fibre-diffraction ... Ng H, Kopka ML and Dickerson RE (2000) The structure of a stable intermediate in the A-DNA helix ...
[11]
B-DNA - an overview | ScienceDirect Topics
The major and minor groove widths are approximately 12 Å and 6 Å, respectively. The base pairs are exposed to solvents through the grooves. The purine C6-N7 ...
[12]
Origins of specificity in protein-DNA recognition - PubMed Central
Ideal B-DNA exhibits a wide and shallow major groove and a narrow and deep minor groove [64] (Figures 1b and 1c). As is evident from Figures 1f and 1g, the ...
[13]
Understanding B-DNA to A-DNA transition in the right-handed DNA ...
B-DNA to A-DNA (B→A) transition depends on various factors such as base composition of a sequence, ion concentration, water activity, etc. At the low relative ...
[14]
Free Energy Landscape of A-DNA to B-DNA Conversion in Aqueous ...
Free Energy Landscape of A-DNA to B-DNA Conversion in Aqueous Solution. J Am ... kcal/mol. A continuum of intermediate structures with no well-defined ...
[15]
Structure and Formation of Z-DNA and Z-RNA - PubMed Central - NIH
Z-conformation nucleic acids adopt a narrower, more elongated double helix with helical rise and diameter of 44.6 Å and 18 Å, respectively, in contrast to both ...
[16]
https://www.nature.com/articles/171740a0
[17]
Molecular Configuration in Sodium Thymonucleate - Nature
FRANKLIN, R., GOSLING, R. Molecular Configuration in Sodium Thymonucleate. Nature 171, 740–741 (1953).
[18]
Deconstructing DNA Beyond the Helix | American Scientist
In the A form, the rungs are angled across the helix axis. In Franklin's images, humidity affected the form of the molecule. The A form was seen at 0–75 percent ...
[19]
The B- to A-DNA Transition and the Reorganization of Solvent ... - NIH
This leads to fewer water molecules required to hydrate A-DNA than B-DNA, and therefore to economy of hydration.
[20]
Improvement of DNA and RNA Sugar Pucker Profiles from ...
We found that the sugar pucker of activated ribonucleotides switches from C2'-endo in the free state to C3'-endo upon binding to an RNA template. This switch ...
[21]
The origin of the A to B transition in DNA fibers and films - Lindsay
In most samples, the transition occurs in three days (after putting the sample at 92% relative humidity) but in highly crystalline samples, the transition may ...
[22]
A crystallographic map of the transition from B-DNA to A-DNA - PNAS
A–D show the view into the major groove of the three base pairs (G2-C11, C3-G10, and G4-C9) from each conformational class superimposed on base pairs from the ...
[23]
A-like guanine-guanine stacking in the aqueous DNA duplex of d ...
Fourthly, the present study extends the vocabulary of DNA secondary structures by the heteronomous duplex of d(GGGGCCCC) in which the B-like strand of oligo(dC) ...
[24]
Heteronomous DNA - PMC - NIH
A fibrous form of poly d(A):poly d(T) has a heteronomous secondary structure which is the first to be confirmed for a polynucleotide duplex: although both ...Missing: like strands
[25]
The structure of a stable intermediate in the A ↔ B DNA helix transition
G-tracts favor the A DNA helix conformation and, depending on the local water activity, can induce a B-to-A DNA transition (10–13). ... 3 Å, intermediate ...
[26]
(PDF) The structure of a stable intermediate in the A <–>B DNA helix ...
Aug 6, 2025 · The percentages of N puckering and helical parameters relevant to A/B geometries for MD simulations and experiments are compared in Table 4.
[27]
Spectroscopic and Molecular Dynamics Evidence for a Sequential ...
... water activity in solution or high relative humidity in fibers. When the water activity is lowered, or the relative humidity of a DNA fiber is <∼75%, B ...
[28]
Sodium and Chlorine Ions as Part of the DNA Solvation Shell
Increased salt concentrations favor the formation of A-DNA and Z-DNA over B-DNA (Saenger, 1984, Nishimura et al., 1986) and salt effects constitute major ...
[29]
A molecular level picture of the stabilization of A-DNA in mixed ...
The transition to A-DNA is rapid and characterized by a drop in the RMSd to cannonical A-DNA from ≈4 Å at 1,350 ps to less than 2.5 Å by 1,500 ps. At 1,350 ps, ...Missing: source | Show results with:source
[30]
Effects of natural and synthetic polyamines on the conformation of ...
Although cellular functions of polyamines are not as yet precisely defined, polyamines are known to induce and stabilize unusual DNA conformations (5–8).
[31]
A Virus that Infects a Hyperthermophile Encapsidates A-Form DNA
The non-enveloped, rod-shaped virus SIRV2 infects the hyperthermophilic acidophile Sulfolobus islandicus which lives at 80° C and pH 3.Missing: dehydration | Show results with:dehydration
[32]
Structures of filamentous viruses infecting hyperthermophilic ... - PNAS
Aug 5, 2020 · A characteristic feature of filamentous archaeal viruses is that their genomic double-stranded DNA (dsDNA) is complexed in A-form (SI Appendix, ...
[33]
APTE: identification of indirect read-out A-DNA promoter elements in ...
Aug 26, 2014 · Although DNA is usually in the canonical B-form it can be induced into A-form by dehydration and certain DNA sequences can naturally adopt an A- ...
[34]
The catalytic mechanism, metal dependence, substrate specificity ...
Nov 20, 2022 · There are three types of RNase H substrates: RNA/DNA hybrids or RNA/DNA hybrid-containing R-loops, RNA–DNA/DNA (Okazaki-like substrates), and DNA-rN(n)-DNA/DNA ...