Fact-checked by Grok 2 weeks ago

Capsid

A capsid is the protein shell that encloses and protects the genetic material (genome) of a virus, serving as a nanoscale container that safeguards the nucleic acid from environmental damage during transmission between host cells.^[1] Composed primarily of multiple copies of one or more viral proteins known as protomers, which self-assemble into larger structural units called capsomeres, the capsid exhibits precise geometric symmetry to achieve stability and efficiency in packaging the genome.^[2] Viral capsids display a variety of shapes, with the most common being icosahedral (a polyhedral structure with 20 triangular faces, providing maximal volume with minimal surface area) and helical (a cylindrical or rod-like form where proteins coil around the elongated nucleic acid), while some viruses feature more complex or irregular architectures.^[3] These shapes are determined by the virus family and contribute to the overall virion morphology, influencing infectivity, stability, and host recognition.^[4] In non-enveloped viruses, the capsid forms the complete outer layer, whereas in enveloped viruses, it lies beneath a lipid membrane derived from the host cell.^[3] Beyond protection, capsids play critical roles in the viral lifecycle, including facilitating genome delivery into new host cells through disassembly (uncoating) upon entry and, in some cases, mediating attachment to host receptors via surface proteins.^[1] The assembly of capsids occurs rapidly and spontaneously in infected cells, driven by protein-protein interactions and often coupled with genome packaging, which requires overcoming electrostatic repulsions between the negatively charged nucleic acid and the positively charged inner capsid surface.^[5] Due to their structural uniformity and mechanical resilience—exhibiting properties like elasticity and rigidity—capsids have become models for studying biomolecular self-assembly and inspire applications in nanotechnology, such as drug delivery vehicles.^[1]

Definition and Composition

What is a Capsid

A capsid is the protein shell that encloses and protects the genetic material of a virus, which can be either DNA or RNA.^[6] This structure serves as the primary protective barrier for the viral genome against environmental damage and host defenses, and it is essential for the virus's ability to infect host cells.^[2] While many viruses are non-enveloped and consist solely of the capsid surrounding the nucleic acid, others are enveloped, where the capsid forms an internal core surrounded by a lipid membrane derived from the host cell.^[3] The capsid is distinct from the nucleocapsid, which refers to the complex of the capsid and its enclosed nucleic acid, and from the virion, which is the complete infectious virus particle that may include an outer envelope in addition to the nucleocapsid.^[2] In non-enveloped viruses, the virion is equivalent to the nucleocapsid, whereas in enveloped viruses, the capsid remains an internal component of the larger virion structure.^[7] The term "capsid" was coined in the mid-20th century, specifically with its first known use in 1959, derived from the Latin word capsa, meaning "box," reflecting its role as a container for the viral genome.^[8] Viral capsids were first visualized in the late 1930s and 1940s using the newly developed electron microscope, which allowed researchers to observe the particulate nature of viruses beyond the resolution of light microscopy.^[9] Capsids vary in size, typically ranging from 20 to 300 nanometers in diameter, depending on the virus type and its architectural complexity.^[10] For example, the poliovirus features a simple naked icosahedral capsid approximately 30 nanometers in diameter, consisting of 60 copies each of four proteins that form a symmetric shell around its RNA genome.^[11] In contrast, the human immunodeficiency virus (HIV) has a more complex conical capsid core about 50-60 nanometers wide at its base, which houses the RNA genome and is surrounded by an envelope in the mature virion.^[12]

Protein Subunits and Capsomeres

Capsid protein subunits, also known as protomers, are the fundamental building blocks of viral capsids, consisting of individual polypeptide chains or small oligomers that self-assemble to form the protective shell around the viral genome.^[2] These protomers are typically encoded by one or a few viral genes and exhibit high sequence variability across virus families, yet they share common structural features that enable stable assembly.^[13] In many cases, protomers aggregate into larger oligomeric clusters called capsomeres, which appear as distinct morphological units under electron microscopy and represent the visible repeating subunits of the capsid surface.^[2] A prevalent structural motif among capsid protomers is the jelly-roll β-barrel fold, an eight-stranded antiparallel β-sheet structure that forms a compact barrel-like domain, providing rigidity and facilitating inter-subunit interactions.^[14] This fold is particularly characteristic of non-enveloped viruses in the Picornavirales order, where the major capsid proteins VP1, VP2, and VP3 each adopt this conformation, with flexible loops and extensions allowing adaptation to different viral architectures.^[15] Chemically, capsid protomers are composed almost entirely of proteins, often as unglycosylated polypeptides but sometimes bearing N- or O-linked glycans that influence stability or host interactions; their molecular weights generally range from 10 to 100 kDa per subunit, enabling efficient packaging within the nanoscale confines of the virion.^[2] Non-enveloped capsids, by definition, exclude lipids, distinguishing them from the lipid-bilayer envelopes of other viruses.^[3] The number of protomers in a capsid varies with viral size and complexity, with the simplest icosahedral structures incorporating 60 identical or quasi-identical subunits arranged in a symmetric shell.^[16] Larger capsids employ multiples of this base number, such as 180 or 540 subunits, to expand surface area while maintaining structural integrity.^[16] A representative example is the foot-and-mouth disease virus (FMDV), an aphtovirus in the Picornaviridae family, whose capsid comprises 60 copies each of four protomers: VP1, VP2, VP3, and VP4.^[17] In FMDV, VP1 protrudes from the surface and mediates receptor binding via its exposed GH loop, VP2 and VP3 form the bulk of the capsomere interfaces with roles in antigenicity and stability, and VP4 resides internally, associating closely with the RNA genome.^[18] These subunits collectively shield the viral genome from nucleases and environmental stressors, ensuring infectivity.^[1]

Symmetry and Architecture

Principles of Viral Symmetry

Viral capsids adopt symmetric architectures to organize multiple copies of protein subunits into stable, efficient enclosures for the viral genome. The primary types of symmetry observed are icosahedral and helical, with some viruses exhibiting more complex or irregular symmetries. Icosahedral symmetry, characterized by five-fold, three-fold, and two-fold rotational axes, predominates in spherical viruses, enabling a closed shell that evenly distributes structural strain across identical subunits. Helical symmetry, in contrast, generates elongated, cylindrical structures ideal for viruses with linear genomes, where subunits align in a repeating spiral pattern around the nucleic acid. These symmetric principles ensure geometric consistency while accommodating the functional needs of diverse viral families.^[3] The biological rationale for symmetry in capsid formation lies in its ability to maximize efficient packing of protein subunits around the irregular shape of the nucleic acid, thereby minimizing free energy during self-assembly and enhancing structural stability. Symmetric arrangements allow a small number of subunit types to form large, closed structures without gaps or overlaps, optimizing resource use in the constrained genetic economy of viruses. This efficiency is particularly crucial for non-enveloped viruses, where the capsid must withstand environmental stresses independently. By enforcing equivalent bonding interactions among subunits, symmetry reduces conformational variability and promotes rapid, error-free assembly in the host cell.^[19]^[20] The foundational framework for understanding these symmetries, particularly icosahedral ones, is the Caspar-Klug theory proposed in 1962, which predicts allowable symmetric structures based on quasi-equivalent interactions between identical protein subunits arranged on an icosahedral lattice. This theory posits that subunits adopt slightly distorted conformations to maintain similar bonding domains, enabling the formation of polyhedral shells with 12 pentavalent vertices and variable hexagonal faces. For icosahedral capsids, asymmetry elements such as pentons—clusters of five subunits at the 12 five-fold rotational vertices—and hexons—clusters of six subunits at the six-fold axes—define the core structural motifs that enforce overall symmetry. The simplest application is seen in T=1 capsids, where 60 subunits form a basic icosahedron without hexagonal elaboration.^[19]^[21] Recent advancements in cryo-electron microscopy (cryo-EM) have revolutionized the visualization of these symmetric principles, achieving near-atomic resolution of capsid arrangements in non-enveloped viruses and revealing subtle deviations or enforcements of symmetry at the molecular level. These high-resolution structures have clarified how symmetric lattices accommodate dynamic processes like genome packaging, with techniques such as single-particle analysis and subtomogram averaging enabling the mapping of subunit interfaces in unprecedented detail. Such insights confirm the predictive power of classical theories while highlighting symmetry's role in functional adaptability.^[22]

Quasi-equivalence and T-numbers

The quasi-equivalence principle, proposed by Donald Caspar and Aaron Klug in 1962, addresses the challenge of assembling icosahedral viral capsids using identical protein subunits in non-identical environments. This principle posits that subunits adopt slightly different conformations to occupy geometrically equivalent positions on the capsid surface, thereby enabling the closure of a symmetric shell while minimizing strain from identical bonding geometries. By allowing local adjustments in subunit bonds and shapes, quasi-equivalence facilitates efficient use of a single protein type to form large, stable structures, as observed in many small icosahedral viruses. Central to this framework is the triangulation number T, which quantifies the size and subunit arrangement in icosahedral lattices. Defined as T = h^2 + hk + k^2, where h and k are nonnegative integers representing steps along the icosahedral lattice vectors, T determines the total number of protein subunits as $60T. For instance, the simplest T=1 structure (where h=1, k=0) consists of 60 subunits arranged in 12 pentamers, while T=3 (e.g., h=1, k=1) expands to 180 subunits, incorporating 12 pentamers and 20 hexamers to increase the capsid radius. This parameterization allows for a systematic classification of capsid architectures, with larger T values corresponding to bigger viruses that accommodate more genetic material. The internal volume of an icosahedral capsid can be approximated as that of a sphere, V \approx \frac{4}{3} \pi r^3, where the radius r scales with \sqrt{T} times a characteristic subunit dimension, reflecting the lattice expansion. This approximation provides a useful estimate for genome packaging capacity, as higher T values yield proportionally larger enclosed volumes suitable for encapsidating longer nucleic acids. For larger T, deviations from strict quasi-equivalence arise, including skew (twisted) lattices where hexamer orientations rotate progressively around pentamers, or merry-go-round classes involving azimuthal twists to relieve steric clashes. These adaptations extend the Caspar-Klug model for complex structures while preserving overall icosahedral symmetry. Recent advances in 2024 have employed computational models based on spherical tiling theory to unfold icosahedral lattices into 2D dihedrons, explicitly predicting subunit shapes and quasi-equivalent interfaces for T=1 to higher values. These models fit experimental structures, revealing how conformational flexibility at interfaces enables assembly across diverse capsid sizes.^[23] Such approaches enhance understanding of structural constraints beyond classical quasi-equivalence. For example, adenovirus employs T=25 to form a large capsid with quasi-equivalent hexamers and pentamers.^[24]

Morphological Types

Icosahedral Capsids

Icosahedral capsids exhibit a polyhedral architecture with icosahedral symmetry, comprising 20 equilateral triangular faces, 12 vertices, and 30 edges, which provides a robust, closed shell for enclosing the viral genome.^[20] These structures typically range in diameter from 25 to 90 nm, allowing accommodation of diverse genome sizes while maintaining structural integrity.^[25]^[26] Icosahedral symmetry is prevalent among most non-enveloped spherical viruses, particularly those in families such as Picornaviridae and Adenoviridae, where it facilitates efficient self-assembly and stability.^[27]^[28] A key structural feature of icosahedral capsids is the arrangement of protein subunits into 12 pentameric clusters at the vertices, which introduce the necessary curvature to form the spherical shape, while the triangular faces are built from hexameric units that fill the surface lattice.^[20] In certain double-stranded DNA viruses, such as herpesviruses, one of the 12 vertices is uniquely occupied by a dodecameric portal complex, which serves as a channel for genome packaging into the procapsid during assembly.^[29] This portal replaces a standard penton and enables directional translocation of the DNA, highlighting adaptations for larger genomes in icosahedral designs.^[30] The poliovirus capsid, from the Picornaviridae family, represents a canonical T=1 icosahedral structure, with a diameter of approximately 30 nm and 60 identical protomers—each comprising four viral proteins (VP1–VP4)—arranged according to quasi-equivalence principles.^[25]^[31] This simple organization ensures high stability without additional morphological elaborations, protecting the single-stranded RNA genome. In comparison, the adenovirus capsid exemplifies a larger, more elaborate T=25 icosahedral form, measuring about 90 nm in diameter and featuring prominent fiber spikes extending from the pentameric vertices to mediate attachment to host cell receptors.^[32]^[26] Recent cryo-electron microscopy (cryo-EM) studies have advanced understanding of icosahedral capsid dynamics, particularly in herpesviruses, by resolving intermediate maturation states that reveal scaffold protein expulsion and portal conformational changes during genome incorporation.^[29] For instance, high-resolution structures of varicella-zoster virus A-capsids, which retain internal scaffolds, illustrate the transitional architecture between procapsid and mature forms, underscoring the role of vertex-specific components in stabilizing these intermediates.^[33]

Helical and Rod-like Capsids

Helical capsids feature protein subunits arranged in a continuous spiral, or helical turns, that wind around the central axis of the viral nucleic acid, forming an elongated cylindrical structure. Unlike closed polyhedral forms, these capsids are typically open-ended at both termini, allowing for scalable assembly where the overall length directly corresponds to the size of the packaged genome. This arrangement enables efficient encapsulation of linear nucleic acids, with the protein subunits interacting closely with the genome to stabilize the virion.^[3] The geometry of helical capsids is characterized by key parameters, including the helix pitch P, which is calculated as P = \mu \times \rho, where \mu represents the number of subunits per helical turn and \rho is the axial rise per subunit along the helix axis. For instance, in tobacco mosaic virus (TMV), \mu = 16.3 subunits per turn and \rho \approx 0.14 nm, yielding a pitch of approximately 2.3 nm. These parameters dictate the tightness and periodicity of the helix, influencing stability and genome binding efficiency.^[34] Helical capsids lack a predefined maximum size, permitting variable lengths based on genome incorporation, and can exhibit either rigid rod-like or flexible filamentous morphologies depending on subunit interactions and environmental conditions. They are prevalent among plant and insect viruses, where such structures facilitate transmission through vectors or mechanical means. In contrast to icosahedral capsids, which require closure at defined vertices, helical forms offer adaptability for elongated genomes without fixed endpoints.^[2] A prominent example is the tobacco mosaic virus (TMV), a rigid helical capsid measuring 300 nm in length and 18 nm in diameter, composed of 2130 identical coat protein subunits arranged helically around its single-stranded RNA genome. Each subunit binds three nucleotides of the RNA, ensuring precise packaging and structural integrity. Recent studies in 2025 have explored conformation-switching mechanisms in RNA virus helical nucleocapsids, such as those in SARS-CoV-2, revealing how dynamic subunit ensembles facilitate self-assembly and phase separation for efficient virion formation. These findings highlight adaptive protein flexibility in promoting genome condensation during helical polymerization.^[35]^[36]^[37]

Complex and Pleomorphic Capsids

Complex capsids deviate from simple icosahedral or helical symmetries by incorporating elongated or hybrid structures, often tailored to specific viral functions such as genome packaging in larger volumes or host attachment. Prolate capsids, a subtype of complex forms, feature an elongated icosahedral shape achieved by extending a cylindrical midsection between two icosahedral end caps, allowing accommodation of oversized genomes in bacterial hosts. For instance, bacteriophage T4 exhibits a prolate head measuring 120 nm in length and 86 nm in width, constructed from 155 hexameric major capsid protein subunits (gp23*) and 11 pentameric vertices (gp24*), with the 12th vertex occupied by a dodecameric portal complex (gp20); this structure follows a triangulation number of T=13 for the end caps and T=20 for the midsection, enabling packaging of a ~171 kb dsDNA genome.^[38] Similarly, bacteriophage φ29 forms a prolate capsid with T=3 symmetry end caps and a Q=5 cylindrical extension, resulting in dimensions of approximately 54 nm in length and 45 nm in width, which supports efficient DNA packaging via a headful mechanism adapted for its Bacillus host.^[39] In complex capsids, additional appendages like tails or spikes integrate with the core shell to form multifunctional assemblies, particularly prominent in tailed bacteriophages of the order Caudovirales, which comprise over 96% of known phages. These viruses feature an icosahedral head connected to a helical tail via a connector, with the tail serving as a syringe-like injector for DNA delivery into bacterial cells; the head, typically 60-100 nm in diameter, houses a single dsDNA molecule of 50 kb or more, while tails vary from non-contractile siphovirus types (e.g., 140 nm long) to contractile myovirus types (e.g., T4's 140 nm tail).^[40] This hybrid symmetry—icosahedral for stability and helical for flexibility—facilitates host-specific adaptations, such as receptor binding at the tail tip, and allows larger overall virion sizes up to 200 nm in length for accommodating extended genomes in diverse bacterial environments.^[41] Pleomorphic capsids exhibit variable, non-rigid morphologies that lack strict symmetry, often enveloped and cone-shaped to enable dynamic assembly during viral budding. In human immunodeficiency virus (HIV), the capsid forms a fullerene cone composed of pleomorphic capsid protein (CA) hexamers and pentamers, with a wide base enclosing the genome and a narrow apex facilitating nuclear import; this irregular structure, averaging 100-120 nm in length, arises from lattice defects and allows flexibility in uncoating during infection.^[42] Such pleomorphism supports adaptations like immune evasion and genome protection in eukaryotic hosts.

Assembly Processes

Self-Assembly Mechanisms

The self-assembly of viral capsids is a spontaneous biophysical process where protein subunits, known as protomers, organize into ordered structures without external energy input, primarily for icosahedral and helical viruses. This process begins with nucleation, the rate-limiting step where a small number of protomers—typically 5 to 12—oligomerize to form a stable core that overcomes kinetic barriers to further growth. According to classical nucleation theory applied to virus capsids, this initial cluster acts as a critical nucleus, with its size determined by the balance between favorable subunit interactions and the entropic cost of aggregation in solution.^[43] Following nucleation, polymerization occurs through the hierarchical addition of protomers or pre-formed capsomeres to the growing shell, driven mainly by non-covalent forces such as hydrophobic interactions between apolar patches on the proteins and hydrogen bonding at interfaces. These interactions provide the specificity and stability needed for the structure to expand symmetrically, with the energy gained from subunit binding favoring the closed-shell configuration over aberrant forms. In icosahedral capsids, this leads to the incorporation of pentameric and hexameric clusters at defined positions to achieve the required curvature.^[44]^[43] The overall energy landscape of capsid self-assembly is characterized by a rugged profile with multiple reversible intermediates, allowing the system to explore configurations and correct errors through local disassembly and reassembly. A key feature is the nucleation barrier, represented as a saddle point in the free energy surface, beyond which downhill polymerization dominates; this reversibility ensures high fidelity in forming closed shells despite the complexity of assembling hundreds of subunits. Computational models of this landscape highlight how thermal fluctuations enable the system to escape kinetic traps, promoting efficient assembly under physiological conditions.^[45]^[46] In vitro studies have provided direct insights into these mechanisms, demonstrating that assembly rates are highly sensitive to environmental factors like salt concentration and pH, which modulate electrostatic repulsion between charged protomers. For instance, tobacco mosaic virus (TMV) coat proteins self-assemble into helical rods in vitro within 10 to 30 minutes under near-neutral pH (around 6-7) and moderate ionic strength (0.1 M), with RNA serving briefly as a template to polarize the growing end and initiate polymerization. These experiments underscore the robustness of intrinsic protein interactions in driving assembly even in controlled settings.^[47]^[48] Recent advances in computational modeling, particularly coarse-grained simulations from 2025, have elucidated the role of stretching and bending energies in the self-assembly of non-enveloped spherical viral capsids, revealing how elastic deformations in protein interfaces facilitate the transition from flexible intermediates to rigid shells. These models integrate molecular dynamics with elasticity theory to predict assembly pathways, showing that minimized bending strain at pentameric facets is crucial for icosahedral closure in viruses like those in the Polyomaviridae family. Additionally, 2025 simulations have demonstrated the spontaneous formation of larger, biologically relevant icosahedral capsids and the role of conformation-switching subunits in RNA virus assembly, advancing understanding of symmetric shell formation.^[49]^[50]^[51]

Role of Scaffolding Proteins and Maturation

Scaffolding proteins serve as temporary internal components that guide the proper assembly of viral procapsids in many double-stranded DNA viruses, including tailed bacteriophages and herpesviruses, by providing a structural template for major capsid protein polymerization.^[52] These proteins form a core lattice within the nascent shell, ensuring geometric accuracy and preventing premature collapse or misassembly. In bacteriophage T4, for instance, internal scaffolding proteins gp21 and gp22 assemble with the portal protein to nucleate the prohead, while gp31 functions as a specialized co-chaperonin homologous to the host GroES, facilitating the ATP-dependent folding of the major capsid protein gp23 within the GroEL chamber.^[38]^[53] Without these scaffolding elements, capsid proteins often form aberrant aggregates rather than closed shells. Procapsid formation begins with the polymerization of major capsid proteins around the scaffolding core and portal complex, resulting in an initial, relatively unstable, and often spherical or near-icosahedral structure that lacks the packaged genome.^[52] This procapsid is characterized by flexible hexamers and pentamers in the shell lattice, allowing for subsequent expansion. Upon initiation of genome packaging, the procapsid undergoes a dramatic conformational rearrangement, expanding in volume by up to twofold to accommodate the incoming DNA and achieve the mature capsid geometry.^[54] This expansion is coupled to scaffolding protein dissociation, which is extruded through channels in the shell as the structure stabilizes.^[55] Maturation of the capsid involves proteolytic processing and large-scale conformational shifts that lock the shell into a robust form capable of protecting the genome. In non-enveloped RNA viruses like picornaviruses, the precursor protein VP0 is autocatalytically cleaved into VP2 and the internal peptide VP4 during or shortly after particle assembly, which rearranges intersubunit interactions to enhance shell rigidity and infectivity.^[56] This cleavage event is essential for converting unstable provirions into mature virions. In tailed bacteriophages such as HK97, maturation proceeds via head expansion and delta-domain cross-linking of the major capsid protein, greatly enhancing thermal stability, with the denaturation temperature increasing by approximately 10°C compared to the procapsid.^[57] These changes collectively transform the flexible precursor into a highly stable icosahedral or elongated shell. Genome packaging in icosahedral dsDNA viruses is powered by pentameric or dodecameric ATPase motors that translocate the DNA through the portal protein channel at the unique vertex of the procapsid, exerting forces up to 60 pN.^[58] In herpesviruses, the portal protein complex serves as the entry point and signal transducer, coordinating motor activity with scaffolding release and capsid expansion to complete maturation.30834-7) This ATP-fueled process not only fills the capsid but also drives the final stabilization, ensuring the particle's integrity for host cell delivery. Recent cryo-electron microscopy studies have illuminated maturation intermediates in small non-enveloped viruses, including parvoviruses, revealing dynamic dissociation of assembly factors that parallel scaffolding release in larger viruses. For example, high-resolution structures of adeno-associated virus (a parvovirus) assembly intermediates highlight conformational transitions in the VP1/VP2/VP3 shell that stabilize the T=1 icosahedral capsid without traditional scaffolding proteins.^[59]

Functions in the Viral Life Cycle

Protection of Genetic Material

The viral capsid serves as a robust physical barrier that shields the enclosed genetic material from diverse environmental threats, including extreme pH levels ranging from 2 to 12, elevated temperatures up to 60°C, enzymatic degradation by nucleases, and mechanical stresses encountered during transmission.^[2] This protective enclosure ensures the genome's integrity in extracellular conditions, such as acidic gastric environments or desiccation in soil. For instance, the capsid of poliovirus exhibits resistance to low pH, enabling the virus to traverse the stomach and reach the intestine for infection.^[60] Similarly, tobacco mosaic virus (TMV) maintains stability in soil for extended periods, with reports of infectivity persisting for up to 50 years in dried plant debris due to its rigid helical structure.^[61] Capsid stability is maintained through strong inter-subunit bonds formed by protein-protein interactions, which collectively withstand the high internal pressure generated by densely packaged DNA, reaching approximately 20 atmospheres, as in herpes simplex virus type 1 (HSV-1).^[62] This pressure arises from the compact coiling of the genome within the confined capsid volume, yet the structure remains intact to prevent premature rupture. The herpes simplex virus type 1 (HSV-1) capsid, for example, endures this internal force through reinforced protein lattices, preventing genome exposure to external nucleases or osmotic shocks.^[63] In addition to physical protection, capsids contribute to immune evasion by presenting surface epitopes that mimic host proteins, thereby reducing recognition by antibodies and complement proteins. Molecular mimicry allows viral surface structures to resemble self-antigens, dampening the host adaptive response during extracellular transit. The enclosed genome remains hidden from humoral immunity until controlled uncoating occurs upon host cell entry, further minimizing detection by pattern recognition receptors.^[3] Recent advances include the development of small-molecule inhibitors in 2024 that target capsid stability in enteroviruses, such as enterovirus 71, to disrupt this protective function and enhance viral susceptibility to environmental degradation. These compounds bind to hydrophobic pockets in the capsid, altering conformational dynamics and promoting premature destabilization outside the host.^[64] This approach highlights the capsid's vulnerability as a therapeutic target, building on the stable forms achieved through self-assembly mechanisms.

Facilitation of Genome Delivery

The capsid facilitates viral genome delivery by enabling initial attachment to host cells through surface proteins that interact with specific receptors. In adenoviruses, the penton base protein, particularly VP1, binds to the coxsackievirus and adenovirus receptor (CAR) on host cell surfaces, initiating infection.^[4] This interaction positions the capsid for subsequent entry, with cryo-EM structures revealing that CAR's D1 domain penetrates the capsid's canyon region, engaging VP1 and VP2 protomers to stabilize binding.^[65] Following receptor engagement, the capsid directs endocytosis and intracellular trafficking, often triggering conformational changes in response to endosomal pH. Many viruses, including adenoviruses and parvoviruses, undergo receptor-mediated endocytosis, where the lowering pH in endosomes (typically 5.0–6.0) induces capsid rearrangements that promote membrane penetration and escape from degradation.^[66] For instance, in human bocavirus, acidic conditions alter capsid stability, exposing hydrophobic regions for fusion with the endosomal membrane.^[67] These pH-dependent shifts ensure the capsid traffics toward the appropriate cellular compartment, such as the perinuclear region, while evading lysosomal destruction.^[68] Uncoating represents a critical step where the capsid partially disassembles to release the genome. In HIV-1, the conical capsid remains intact through the cytoplasm and docks at the nuclear pore complex (NPC), where it undergoes uncoating to permit reverse-transcribed DNA entry into the nucleus.^[69] Recent studies show that the capsid's hexameric lattice interacts with NPC proteins like NUP153, triggering disassembly without complete fragmentation, thus protecting the genome until the final delivery site.^[70] This regulated uncoating is essential for efficient infection, as premature disassembly exposes the genome to host defenses. In some viruses, dedicated portals or channels within the capsid structure serve as exit sites for genome injection. Bacteriophage T4 employs a tail apparatus connected to a portal vertex in the icosahedral head, which acts as a channel for DNA ejection into the bacterial host following tail contraction upon receptor binding.^[38] The portal protein complex, formed by gp20 dodecamers, facilitates unidirectional DNA translocation driven by ATP-powered motors during packaging and reversal for delivery.^[71] Recent advances in adeno-associated virus (AAV) capsid engineering have enhanced genome delivery for therapeutic applications. In 2025, directed evolution and machine learning approaches produced variants like MyoAAV 2A, achieving up to 128-fold increased transduction efficiency in skeletal muscle, enabling more precise and potent gene delivery while minimizing off-target effects.^[72]

Evolutionary Origins and Diversity

Hypotheses on Capsid Origins

The origins of viral capsids are hypothesized to trace back to the earliest stages of life on Earth, with two primary theories dominating the discussion: the virus-first model integrated with the RNA world hypothesis and the escape hypothesis. Under the virus-first model, capsids emerged prior to cellular life as protective enclosures for primordial genetic material. In this scenario, aligned with the RNA world hypothesis, self-replicating RNA molecules in a prebiotic environment were encapsulated by simple, self-assembling peptides encoded by early genes. These peptides formed basic icosahedral structures, such as those seen in modern small viruses like satellite tobacco mosaic virus, providing compartmentalization, protection from degradation, and enhanced mobility for RNA replicons in a ribonucleoprotein (RNP)-dominated world. This process is thought to have bridged the gap between RNA-based replication and more complex cellular metabolism, with capsids evolving from single-gene products that spontaneously assembled into hollow spheres around ~1 kb RNA genomes.^[73] In contrast, the escape or progressive hypothesis proposes that viral capsids arose from cellular proteins that were co-opted and adapted for independent replication outside host control. According to this view, early viruses "escaped" from cellular genomes, recruiting host-derived coat proteins to form protective shells around their nucleic acids. Examples include structural similarities between viral capsid proteins and bacterial secretion system components, such as those in type VI secretion systems, which form tubular or shell-like assemblies for protein export. Broader evidence supports multiple independent origins, with viral capsids evolving from diverse cellular ancestors like nucleoplasmins (involved in chromatin organization) and carbohydrate-binding modules in glycoside hydrolases, which share beta-barrel architectures suitable for enclosure functions. This hypothesis emphasizes that viruses did not precede cells but emerged as parasitic entities within established cellular lineages.^[74]^[75] Capsids are estimated to have first appeared 3 to 4 billion years ago, contemporaneous with the emergence of life and predating the diversification of modern viral families. This ancient timeline is inferred from the deep evolutionary conservation of capsid protein folds and their integration into early metabolic networks. A pivotal line of evidence is the jelly-roll fold, a beta-sheet structure ubiquitous in icosahedral and other capsid proteins across viruses infecting bacteria, archaea, and eukaryotes, as well as homologs in cellular proteins. This fold's presence across life's domains points to extensive horizontal gene transfer in primordial ecosystems, allowing viral-like particles to disseminate widely before the last universal common ancestor.^[76]^[74] Genomic analyses from 2023 to 2025 have strengthened these connections by identifying ancient gene transfer events between viruses and archaeal/eukaryotic hosts. For instance, a 2024 study of eukaryotic genomes identified major capsid protein homologs from mirusviruses across diverse eukaryotic supergroups, including early-diverging lineages, indicating broad host range and insertions before the last eukaryotic common ancestor (pre-LECA). These findings underscore a shared ancestry for capsid structures, with viral genes integrated into host proteomes long before modern virus diversity arose.^[77]

Conservation and Variation in Capsid Structures

Capsid structures exhibit remarkable conservation in core protein folds across diverse viral lineages, reflecting evolutionary constraints on assembly efficiency and stability. In tailed bacteriophages, the HK97 fold serves as a foundational motif in major capsid proteins, characterized by axial and peripheral domains that enable shell formation through covalent cross-linking during maturation.^[78] This fold is ubiquitous among dsDNA tailed phages, forming the basis of icosahedral or prolate heads in families like Siphoviridae and Myoviridae.^[79] Similarly, the double jelly-roll (DJR) fold predominates in many icosahedral capsids, consisting of two β-barrel domains per protomer that assemble into trimers with pseudo-sixfold symmetry, as seen in adenoviruses and polyomaviruses.^[80] These conserved folds extend across Baltimore classification groups, with the DJR motif appearing in both Group I (dsDNA) and Group II (ssDNA) viruses, underscoring a shared architectural heritage despite genomic differences.^[81] Despite these conserved elements, capsid architectures display significant variation tailored to viral lifestyles and transmission strategies. Helical capsids, prevalent in positive-sense single-stranded RNA (+ssRNA) viruses such as tobamoviruses, wind nucleic acid into a rod-like cylinder using a single coat protein species, allowing flexible packaging of elongated genomes.^[2] In contrast, complex capsids in dsDNA phages like T4 feature elongated heads with tail fibers and baseplates, incorporating multiple protein types for host recognition and injection.^[2] Retroviruses (Group VI) exhibit pleomorphic capsids, often conical or spherical, formed by Gag polyproteins that disassemble post-entry to facilitate reverse transcription, adapting to enveloped virions with variable morphology.^[82] Evolutionary drivers of this variation include host adaptation and genome size constraints, which select for structural modifications enhancing infectivity and stability. Larger genomes in giant viruses correlate with expanded capsid dimensions, achieved through gene duplication events that increase the triangulation number (T-number) in icosahedral symmetry, allowing quasi-equivalent subunit arrangements for bigger shells.^[83] Host shifts impose selective pressures, favoring mutations in surface epitopes for immune evasion or receptor binding, while genome expansion via duplications amplifies structural genes to support diverse assembly pathways.^[84] Phylogenetic analyses of capsid genes reveal monophyletic clades within families, tracing diversification from common ancestors. In Parvoviridae (Group II), capsid protein sequences form distinct clades reflecting host specificity, with recent structural AI-assisted modeling of cryo-EM data elucidating evolutionary divergences in assembly intermediates and maturation.^[85] These trees highlight vertical inheritance punctuated by horizontal gene transfer, linking capsid evolution to virome dynamics. A 2025 review on non-enveloped spherical viruses highlights cryo-EM's role in resolving assembly pathways and providing high-resolution structural details, contributing to a deeper understanding of their biology and phylogeny.^[86] Such insights underscore the balance between structural conservation for functional reliability and variation for ecological niche exploitation.

References

[1]
Viral capsids: Mechanical characteristics, genome packaging and ...
Viral capsids are nanometre-sized containers that possess complex mechanical properties and whose main function is to encapsidate the viral genome in one host.
[2]
Structure and Classification of Viruses - Medical Microbiology - NCBI
Helical nucleocapsids consist of a helical array of capsid proteins (protomers) wrapped around a helical filament of nucleic acid. Icosahedral morphology is ...Morphology · Icosahedral Symmetry · Virus Classification
[3]
Virus Structure and Classification - PMC - PubMed Central
The virus capsid functions to protect the nucleic acid from the environment, and some viruses surround their capsid with a membrane envelope. Most viruses have ...
[4]
Virus Capsid - an overview | ScienceDirect Topics
Viral capsids are the protein cage derived from the protein shell of a virus, and can have different shapes, sizes, and protein subunits, depending on the ...
[5]
Viral genome structures are optimal for capsid assembly - PMC - NIH
In many viral families, a protein shell called a capsid forms around the viral genome during the assembly process. However, capsids can also assemble around ...
[6]
virus | Learn Science at Scitable - Nature
When a virus particle is independent from its host, it consists of a viral genome, or genetic material, contained within a protein shell called a capsid. In ...
[7]
Capsids and nucleocapsids - Virology Blog
Mar 17, 2022 · Below are two different types of capsid ... A nucleocapsid is defined as the nucleic acid-protein assembly within the virus particle.
[8]
CAPSID Definition & Meaning - Merriam-Webster
Word History ; Etymology. French capside, from Latin capsa case + French -ide -id entry 2 ; First Known Use. 1959, in the meaning defined above ; Time Traveler.Missing: virology coined 1950s
[9]
Viral detection by electron microscopy: past, present and future - PMC
EM was first developed in the 1930s, by physicists in various countries, including Germany, in particular (reviewed in Haguenau et al., 2003).
[10]
10.2: Size and Shapes of Viruses - Biology LibreTexts
Aug 31, 2023 · Most viruses range in size from 5 to 300 nanometers (nm), in recent years a number of giant viruses, including Mimiviruses and Pandoraviruses with a diameter ...Size · Shapes
[11]
Virus Classification | Biology for Majors II - Lumen Learning
The capsid of the (a) polio virus is naked icosahedral; (b) the Epstein-Barr virus capsid is enveloped icosahedral; (c) the mumps virus capsid is an ...
[12]
Structure, Function, and Interactions of the HIV-1 Capsid Protein - PMC
The capsid core is responsible for the safe delivery of the viral genome and reverse-transcription machinery from the periphery of the cytoplasm to ...
[13]
[PDF] All-Atom Molecular Dynamics of Virus Capsids as Drug Targets
Apr 29, 2016 · Virus capsids can be composed of one or more types of protein building blocks (protomers), arranged according to well-defined geometric ...
[14]
The Picornaviridae Family: Knowledge Gaps, Animal Models ...
Oct 18, 2023 · Each protein contains an 8-stranded antiparallel β-barrel core with a “jelly-roll' fold [48]. The N-termini of the proteins are found within ...
[15]
Identification of Capsid/Coat Related Protein Folds and Their Utility ...
Mar 9, 2017 · The Picornavirus-Like Lineage. The Picornavirus-like lineage is characterized by the “jelly-roll” or “β-barrel” fold, which is commonly seen ...
[16]
Learn: Paper Models: Quasisymmetry in Icosahedral Viruses
The smallest examples have a capsid composed of 60 copies of a protein, arranged in icosahedral symmetry. Larger viruses have two options for building a larger ...
[17]
Foot-and-Mouth Disease Virus Assembly
The mature picornavirus comprises 60 copies each of four structural proteins, termed VP1, VP2, VP3, and VP4, which encapsidate a single, positive-sense RNA ...
[18]
Advances in Foot-and-Mouth Disease Virus Proteins Regulating ...
Oct 8, 2020 · Four structural proteins (VP4, VP2, VP3, and VP1) encoded by the P1 region of FMDV, mainly form the icosahedral capsid of virus particles: VP1 ...Abstract · Introduction · FMDV Proteins Regulate Host...
[19]
Physical Principles in the Construction of Regular Viruses
Physical Principles in the Construction of Regular Viruses. D. L. D. Caspar and; A. Klug*. + ... 1962. 27: 1-24. » ExcerptFree; Full Text (PDF)Free to you ...
[20]
Principles of Virus Structural Organization - PMC - PubMed Central
Capsid organization in many small plant and animal viruses follows T = 3 icosahedral symmetry with 180 chemically identical subunits (Fig. 3.7). In a T = 3 ...
[21]
A quasi‐atomic model of human adenovirus type 5 capsid
Their icosahedral capsids are composed of three major proteins: the trimeric hexon forms the facets and the penton, a noncovalent complex of the pentameric ...
[22]
How structural biology has changed our understanding of ...
Sep 18, 2024 · In 1956, Crick and Watson proposed that viruses should have cubic symmetry, and hence, there should be viral particles with tetrahedral, ...
[23]
Explicit description of viral capsid subunit shapes by unfolding ...
Nov 14, 2024 · Many spherical viral capsids adopt icosahedral structures, which is fully characterized by 60 symmetry operations. To elucidate the mechanism of ...
[24]
https://pmc.ncbi.nlm.nih.gov/articles/PMC8156859/
[25]
Four decades of adenovirus gene transfer vectors - Cell Press
Apr 2, 2025 · Adenoviruses (Ad) are 90- to 100-nm nonenveloped viruses comprising an icosahedral protein capsid encompassing a double-stranded DNA genome.
[26]
https://www.cell.com/molecular-therapy-family/molecular-therapy/fulltext/S1525-0016%2825%2900271-0
[27]
Basics of virology - PMC - PubMed Central
The nearly spherical non-enveloped icosahedral capsids are 28–30 nm in diameter. ... Picornavirus virions are non-enveloped icosahedral capsids 28–30 nm in ...Missing: prevalence | Show results with:prevalence
[28]
Cryo-electron microscopy structures of capsids and in situ portals of ...
Apr 11, 2023 · Taken together, the conformational changes to the portal in response to DNA packaging provide structural insight into the head-full mechanism of ...
[29]
Structural basis for genome packaging, retention, and ejection in ...
Jul 27, 2021 · We present the in situ structures of the symmetry-mismatched portal and the capsid vertex-specific components (CVSCs) of HCMV.
[30]
A human monoclonal antibody binds within the poliovirus receptor ...
Oct 10, 2023 · The viral capsid is about 30 nm in diameter and is comprised of four structural capsid proteins (VP 1–4) with T = 1 (pseudo T = 3) icosahedral ...
[31]
The Structure of the Human Adenovirus 2 Penton: Molecular Cell
All adenoviruses exhibit the same overall capsid architecture. The T = 25 icosahedral capsid is approximately 1000 Å in diameter and consists primarily of ...
[32]
Cryo-EM structure of the varicella-zoster virus A-capsid - Nature
Sep 22, 2020 · DNA-packing portal and capsid-associated tegument complexes in the tumor herpesvirus KSHV. Cell 178, 1329–1343 (2019). Article CAS PubMed ...
[33]
Inoviridae - an overview | ScienceDirect Topics
For TMV, μ = 16.3; that is, there are 16.3 coat protein molecules per helix turn, and p = 0.14 nm. Therefore, the pitch of the TMV helix is 16.3 × 0.14 = 2.28 ...
[34]
TMV Particles: The Journey From Fundamental Studies to ...
The genomic RNA is encapsidated by approximately 2130 copies of the CP to give virus particles with helical symmetry. The particles are hollow cylinders 300 nm ...
[35]
Molecule of the Month: Tobacco Mosaic Virus - PDB-101
Notice how the RNA is bound in a groove in the protein near the center of the ring. Three nucleotides bind to each protein subunit. The image on the right ...
[36]
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head ...
The SARS-CoV-2 N-protein self-assembles and undergoes PS with RNA [17] for assembling vRNP complexes and potentially facilitating viral assembly [17]. Liquid– ...<|control11|><|separator|>
[37]
Bacteriophage T4 Head: Structure, Assembly, and Genome Packaging
Feb 14, 2023 · The bacteriophage T4 virion (A) has a 120 × 86 nm prolate capsid packaged with ~171 kb linear double-stranded genomic DNA. The major capsid ...
[38]
Structural changes of bacteriophage ϕ29 upon DNA packaging and ...
Proheads (Figure 1) consist of the major capsid protein (gp8), the scaffolding protein (gp7), the head fiber protein (gp8.5), the head‐tail connector (gp10), ...
[39]
Tailed bacteriophages: the order caudovirales - PubMed
Tailed bacteriophages have a common origin and constitute an order with three families, named Caudovirales. Their structured tail is unique.
[40]
A Common Evolutionary Origin for Tailed-Bacteriophage Functional ...
Sep 1, 2011 · Bacteriophages belonging to the order Caudovirales possess a tail acting as a molecular nanomachine used during infection to recognize the ...
[41]
Structural insights into HIV-2 CA lattice formation and FG-pocket ...
Feb 25, 2025 · One of the striking features of human immunodeficiency virus (HIV) is the capsid, a fullerene cone comprised of pleomorphic capsid protein ...
[42]
Advances in AAV capsid engineering: Integrating rational design ...
May 7, 2025 · These strategies have yielded novel capsids with improved transduction efficiency, reduced immunogenicity, and enhanced tissue targeting.Missing: pleomorphic | Show results with:pleomorphic
[43]
Structural studies of Parvoviridae capsid assembly and evolution
In 2025, AlphaFold2-based modeling was used to optimize AAV Vectors for hemophilia A gene therapy, facilitating the engineering variants with improved factor ...Missing: pleomorphic | Show results with:pleomorphic
[44]
Classical Nucleation Theory of Virus Capsids - ScienceDirect.com
A fundamental step in the replication of a viral particle is the self-assembly of its rigid shell (capsid) from its constituent proteins.
[45]
Principles for enhancing virus capsid capacity and stability from a ...
Oct 2, 2019 · These structural elements are locked together using a complicated web of hydrogen bonds, salt bridges, and hydrophobic interactions that latches ...
[46]
A reaction landscape identifies the intermediates critical for self ...
In this paper we describe a reaction landscape for the assembly of the capsids of icosahedral viruses that applies equally well to assembly of any closed ...
[47]
Kinetic Description of Viral Capsid Self-Assembly Using Mesoscopic ...
In the context of VCSA, the landscape features an unstable or saddle point state corresponding to the nucleation barrier that must be surmounted to self- ...
[48]
Partial reconstitution of tobacco mosaic virus - ScienceDirect.com
TMV reconstitutes rapidly in vitro at 24°; a yield of 30% was obtained after 30 min of reconstitution. The maximum yield was around 60%, reached after 7 ...
[49]
Physical Regulation of the Self-Assembly of Tobacco Mosaic Virus ...
The ionic strength in the experiments was 0.1 M, and the total protein concentrations are 4.48 g/l at pH 6.37, 9.41 g/l at pH 6.75, and 5.45 g/l at pH 7.50.Missing: time | Show results with:time
[50]
General elasticity theory for virus assembly - BPS2025 - Cell Press
Feb 13, 2025 · Additionally, we explore the processes of capsid assembly and disassembly using our coarse-grained molecular dynamics model. Article metrics ...
[51]
Structural transitions during the scaffolding-driven assembly of a ...
Oct 24, 2019 · Assembly of tailed bacteriophages and herpesviruses starts with formation of procapsids (virion precursors without DNA).
[52]
Structure, Assembly, and DNA Packaging of the Bacteriophage T4 ...
gp31 replaces the GroES cochaperonin utilized for folding the 10–15% of E. coli proteins that require folding by the GroEL folding chamber. Although T4 gp31 and ...
[53]
Assembly and Maturation of the Bacteriophage Lambda Procapsid
Upon packaging 15–20% of the viral genome, the procapsid undergoes an expansion process, which increases the capsid volume ∼ 2-fold and affords a more ...<|separator|>
[54]
Herpesvirus Capsid Assembly: Insights from Structural Analysis - PMC
Aug 1, 2012 · The procapsid is found to have openings in the floor layer which are closed in the mature capsid (arrows in f and g). Once the procapsid is ...Herpesvirus Capsid Assembly... · Capsid Structure · Capsid Assembly Pathway
[55]
Picornavirus Morphogenesis | Microbiology and Molecular Biology ...
Sep 2, 2014 · With most picornaviruses, the final step of morphogenesis is accomplished by the cleavage of VP0 into VP2 and VP4 (Fig. 3C, PV step VII). This ...
[56]
Crosslinking renders bacteriophage HK97 capsid maturation ...
While it is plausible that crosslinks might enhance capsid stability, this proposition has not been addressed directly, nor has been it determined whether an ...
[57]
Single-molecule packaging initiation in real time by a viral DNA ...
Tailed bacteriophages and herpes viruses use powerful molecular machines to package their genomes into a viral capsid using ATP as fuel.Results · Quantifying Dna Packaging... · Atpase-Defective Mutant...
[58]
The Structures and Functions of Parvovirus Capsids and Missing ...
Jun 27, 2023 · The parvovirus capsid's apparent simplicity likely conceals important functions carried out by small, transient, or asymmetric structures.Missing: maturation | Show results with:maturation
[59]
Virus entry: molecular mechanisms and biomedical applications
Conformational changes in the viral proteins that drive entry are typically initiated by high-affinity interactions with receptors, or changes in pH after ...
[60]
pH-Induced Conformational Changes of Human Bocavirus Capsids
During infection and trafficking, HBoVs encounter different cellular environments. HBoV1 is exposed to the mild acidic pH conditions in the respiratory tract ...
[61]
Viral escape from endosomes and host detection at a glance
Aug 3, 2018 · A variety of cellular factors trigger the conformational rearrangements in viral capsid proteins that drive membrane fusion and pore formation ( ...Introduction · Receptor Binding · Cellular Antiviral Defenses...<|separator|>
[62]
Nuclear pore passage of the HIV capsid is driven by its unusual ...
Oct 9, 2025 · It was long believed that the viral capsid (60 nm × 120 nm) was too large for NPC passage, that capsid uncoating and release of the HIV genome ...
[63]
Molecular Biology of Bacteriophage T4 - ASM Journals
One vertex of the prohead contains a “portal” structure, through which DNA passes both during packaging and later ejection, and to which the tail eventually ...
[64]
Advances in AAV capsid engineering: Integrating rational design ...
May 7, 2025 · These strategies have yielded novel capsids with improved transduction efficiency, reduced immunogenicity, and enhanced tissue targeting.
[65]
Virus-First Theory Revisited: Bridging RNP-World and Cellular Life
The virus-first theory presents a model in which viral lineages emerged before cells. This proposal aims to give the theory greater relevance.Missing: escape | Show results with:escape
[66]
Multiple origins of viral capsid proteins from cellular ancestors - PNAS
We show here that many, if not all, capsid proteins evolved from ancestral proteins of cellular organisms on multiple, independent occasions.Missing: primary | Show results with:primary
[67]
Common Evolutionary Origin of Procapsid Proteases, Phage Tail ...
Sep 22, 2016 · The structure suggests that these proteases share common evolutionary origin with proteins forming the tubes of phage tails and bacterial type.
[68]
The ancient Virus World and evolution of cells | Biology Direct
Sep 19, 2006 · Their viral hallmark genes have had plenty of opportunity in the last three-four billion years to make their way into one or another host genome ...Missing: timeline | Show results with:timeline
[69]
Ancient Host-Virus Gene Transfer Hints at a Diverse Pre-LECA ...
Apr 29, 2025 · Our results show that viral enzymes form clades that are typically adjacent to eukaryotes, suggesting that they originate prior to the emergence ...
[70]
Eukaryotic genomic data uncover an extensive host range of ...
Jun 17, 2024 · Two recent studies investigating the viral signals in eukaryotic genomes ... Multiple origins of viral capsid proteins from cellular ancestors.
[71]
The amazing HK97 fold: versatile results of modest differences - PMC
Mar 8, 2019 · The conserved elements are 1) A-domain (axial domain), 2) the P-domain (peripheral domain), E-loop (extended Loop), and N-arm (N-terminal arm), ...
[72]
A structural dendrogram of the actinobacteriophage major capsid ...
Mar 2, 2023 · To date, all the structurally characterized tailed bacteriophages use the HK97-fold (Figure 1A) as the foundational block to build the capsid ( ...
[73]
Conservation of major and minor jelly-roll capsid proteins in Polinton ...
Apr 29, 2014 · Here, we show that both Polintons and Tlr elements encode two key virion proteins, the major capsid protein with the double jelly-roll fold and ...
[74]
Natural history of eukaryotic DNA viruses with double jelly-roll major ...
Finally, the polinton pPolB gene has been horizontally transferred to insect parvovirids yielding a group of viruses with linear single-stranded DNA (ssDNA) ...
[75]
Virus Morphology - an overview | ScienceDirect Topics
Viral capsids have either a helical (springlike) or icosahedral-based (cuboidal or spherical) symmetry. A. Helical Capsids. Helical capsids are usually flexible ...Missing: rationale | Show results with:rationale
[76]
Gene duplication as a major force driving the genome expansion in ...
Nov 21, 2023 · Our data suggest that the larger size of the genome can be attributed to an unprecedented number of duplicated genes. Further investigation of ...
[77]
The evolutionary drivers and correlates of viral host jumps - Nature
Mar 25, 2024 · These results indicate that, on average, 'generalist' multihost viruses experience lower degrees of adaptation when jumping into new vertebrate ...
[78]
Recent advances in the structure and assembly of non-enveloped ...
Feb 20, 2025 · This review seeks to summarize current literature regarding the structure and assembly of the NSVs and discusses how the data has facilitated a deeper ...