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Vaginal process

The processus vaginalis, commonly referred to as the vaginal process, is a blind-ended evagination of the parietal peritoneum that develops during fetal life as part of the inguinal canal formation, primarily to accommodate the descent of the gonads into the scrotum in males or the labia majora in females.^[1] This structure originates from the peritoneal cavity and extends through the inguinal canal, guided by the gubernaculum, typically between the 7th and 9th months of gestation.^[2] In males, it precedes the testis during its migration, forming a protective sheath that later contributes to the tunica vaginalis surrounding the testis; in females, it is shorter and analogous to the canal of Nuck, which connects to the round ligament of the uterus.^[3] Normally, the processus vaginalis undergoes obliteration shortly after birth, sealing off the peritoneal connection to prevent abdominal contents from entering the scrotum or inguinal region, though patency persists in approximately 80% of newborns, decreasing to 20% in adults. Failure of complete closure of the processus vaginalis can lead to significant clinical conditions, including indirect inguinal hernias and hydroceles; in females, this may manifest as hydrocele of the canal of Nuck or inguinal hernias.^[1] In males, a patent processus vaginalis allows peritoneal fluid or bowel loops to protrude into the scrotum, manifesting as a communicating hydrocele or hernia sac, which is a common pediatric surgical issue often requiring herniorrhaphy.^[4] In females, persistence of this structure, known as the canal of Nuck, is rarer but can result in cystic masses or herniation due to its attachment to the uterus via the round ligament, potentially causing pain or swelling in the groin or labia.^[5] Understanding the processus vaginalis is crucial in pediatric and general surgery, as its anomalies account for a substantial portion of inguinal pathologies, with ultrasound serving as a primary diagnostic tool to assess patency and associated disorders.^[6]

Anatomy

Gross structure

The vaginal process, or processus vaginalis, is defined as a finger-like outpouching of the parietal peritoneum that protrudes from the abdominal cavity into the inguinal canal.^[3] This evagination forms a blind-ended peritoneal diverticulum, serving as a conduit during testicular descent.^[7] Composed of a thin serous membrane, the vaginal process is lined by a single layer of mesothelium, which consists of simple squamous epithelial cells resting on a connective tissue basement membrane; this structure creates a potential space capable of containing fluid or other contents.^[3] The mesothelial lining provides a smooth, lubricated surface typical of peritoneal extensions.^[7] In its developmental extent, the vaginal process originates at the deep inguinal ring and extends through the inguinal canal, reaching the scrotum in males or the labium majus in females.^[8] In normal adult males, the proximal portion typically obliterates after development, while the distal segment persists as the tunica vaginalis testis, comprising a parietal layer lining the scrotal wall and a visceral layer directly investing the testis.^[3] This remnant encloses a small amount of serous fluid, maintaining a serous sac around the testicular structures.^[7]

Relations and location

The processus vaginalis originates as a peritoneal evagination at the deep inguinal ring, an opening in the transversalis fascia located just above the midpoint of the inguinal ligament and lateral to the inferior epigastric vessels.^[4] It then traverses the inguinal canal, passing through the layers of the anterior abdominal wall, including the internal oblique muscle and external oblique aponeurosis, before terminating at the superficial inguinal ring, a triangular defect in the external oblique aponeurosis situated superolateral to the pubic tubercle.^[4] Beyond the superficial ring, it extends into the scrotum in males or the labia majora in females, forming a potential space lined by peritoneum.^[1] In its course through the inguinal canal, the processus vaginalis lies posterior to the anterior wall, which is reinforced by the external oblique aponeurosis, and anterior to the posterior wall composed of transversalis fascia and the conjoint tendon medially.^[4] In males, it maintains a close spatial relationship with the spermatic cord, serving as the conduit through which the cord and testis descend, ultimately contributing to the formation of the tunica vaginalis that partially envelops the testis.^[3] Laterally, its entry at the deep ring remains positioned to the lateral side of the inferior epigastric vessels, distinguishing its path from direct inguinal hernias that occur medial to these vessels.^[4] In normal anatomy, the processus vaginalis exhibits variations in size and patency following its formation, with complete obliteration being the typical outcome after descent, though partial patency or small residual sacs may persist asymptomatically in approximately 40% of males at two years of age, decreasing to about 20% in adults, and in a lower proportion of females (around 3-10%).^[1]^[9] These variations often involve a shortened or incomplete closure proximally near the deep ring while remaining open distally around the testis or round ligament, reflecting individual differences in the extent of peritoneal resorption without altering the structure's positional relations.^[3]

Embryology

Formation and early development

The vaginal process, also known as the processus vaginalis, originates as an evagination of the parietal peritoneum from the caudal abdominal wall during early fetal development.^[10] This outpouching forms bilaterally as a peritoneal diverticulum, typically beginning around the 8th week of gestation.^[1] By the 9th week, it is clearly evident as a distinct structure, marking the initial phase of its development prior to more advanced gonadal positioning.^[10] The formation of the vaginal process is primarily stimulated by the elongation of the gubernaculum, a mesenchymal band that attaches to the caudal pole of the developing gonad and extends toward the future genital swellings.^[10] During gonadal ridge formation, which commences around the 5th to 6th week, the gubernaculum pulls the peritoneum forward, inducing the evagination and guiding the peritoneal sac's protrusion into the developing inguinal region.^[11] This process creates a pathway independent of later hormonal influences, ensuring the structure's establishment in both male and female embryos before sex differentiation becomes prominent.^[10] In its early stage, the vaginal process appears as a blind-ended peritoneal sac that protrudes bilaterally toward the scrotal or labial swellings, without yet enclosing any gonadal tissue.^[1] This sac remains open at its abdominal end while closed at the distal tip, setting the stage for subsequent events in gonadal migration.^[11] Its appearance by the 9th week precedes the full transabdominal phase of gonadal descent, which occurs between weeks 8 and 15.^[10]

Descent and obliteration

The descent of the gonads, particularly the testes in males, involves the processus vaginalis playing a crucial role in guiding the structures through the inguinal canal into the scrotum during the inguinoscrotal phase of development, which occurs between the 7th and 9th months of gestation (approximately 25-40 weeks).^[10] The gubernaculum, a ligamentous structure attaching the gonad to the scrotal swellings, undergoes a swelling reaction followed by shortening and contraction, pulling the testis caudally while the processus vaginalis—a peritoneal evagination—precedes and envelops it, creating a potential space that develops into the tunica vaginalis serosa around the testis upon arrival in the scrotum.^[12] This mechanism is androgen-dependent, with testosterone and dihydrotestosterone stimulating gubernacular regression via the genitofemoral nerve and calcitonin gene-related peptide (CGRP), ensuring coordinated migration; in females, the process is less androgen-influenced, with the processus vaginalis forming the canal of Nuck that accompanies the round ligament of the uterus to the labia majora, while the ovaries descend only to the pelvis via the gubernaculum, though details on gubernacular dynamics remain incompletely characterized.^[12] Following successful descent, the processus vaginalis undergoes partial obliteration to prevent peritoneal communication, beginning in the third trimester with programmed smooth muscle cell death and continuing postnatally.^[10] The proximal portion, near the deep inguinal ring, typically closes first by sealing off the peritoneal connection, while the distal segment persists as a closed sac forming the tunica vaginalis around the testis in males; this process is complete in the majority of cases by infancy, with full obliteration often achieved by 1-2 years of age.^[1] In females, obliteration similarly involves proximal closure and atresia of the canal of Nuck, though it proceeds without the persistent distal sac seen in males due to the absence of testicular enclosure.^[1] Hormonal regulation of this phase involves a decline in androgen levels post-descent, facilitating apoptotic closure, with CGRP aiding the final sealing in males.^[12]

Sex differences

In males

In adult males, the processus vaginalis typically undergoes partial obliteration, with the proximal portion completely closing in approximately 80-90% of cases by adulthood, while the distal portion persists as the tunica vaginalis testis.^[13] This remnant forms a closed serous sac that envelops the anterior and lateral aspects of the testis and epididymis, except at their posterior borders where attachments to the spermatic cord occur.^[14] The tunica vaginalis consists of two distinct layers: the parietal layer, which lines the inner surface of the scrotum and extends superiorly along the distal spermatic cord, and the visceral layer, which adheres closely to the tunica albuginea of the testis and the epididymis.^[15] These layers create a potential space containing a small amount of serous fluid that functions as a lubricant, enabling free movement of the testis within the scrotum for protection against mechanical stress and temperature regulation.^[16] In relation to the spermatic cord, the tunica vaginalis derives from the distal evagination of the processus vaginalis, which originally precedes testicular descent and encloses key cord structures—including the ductus deferens, testicular artery and veins, and nerves—within the internal spermatic fascia, while the cremaster muscle remains external in its own fascial layer.^[17]

In females

In females, the processus vaginalis, homologous to the structure in males that forms the tunica vaginalis, develops as a peritoneal outpouching accompanying the gubernaculum during embryogenesis but undergoes complete regression due to the absence of ovarian descent into the inguinal canal.^[1] This vestigial extension, known as the canal of Nuck when patent, typically obliterates fully during early postnatal life, leaving no persistent peritoneal sac in the adult inguinal region.^[18] Unlike in males, where partial persistence is common to envelop the testes, the female processus vaginalis closes without forming a functional cavity, reflecting the ovaries' intra-abdominal position.^[1] The final structure in adult females consists of fibrous remnants integrated with the round ligament of the uterus, which terminates in the labia majora; these remnants are minimal and non-peritoneal, with no clinical significance in the absence of patency.^[19] Complete obliteration occurs in two stages—first at the deep inguinal ring, followed by atresia of the canal—resulting in a structure that is smaller and more rudimentary than its male counterpart.^[1] Rare small fibrous strands may persist near the labia majus, but no sac-like extension remains.^[18] In adults, the obliterated processus vaginalis serves no physiological function, as the lack of gonadal migration precludes any role in peritoneal investment or support.^[1] Patency, if present, may manifest as the canal of Nuck and predispose to rare conditions like hydroceles, but this is an anomaly rather than the norm.^[1] Prevalence studies indicate that while up to 60% of female infants may have a patent processus vaginalis at birth, obliteration is typically complete by age two, leading to near-total closure in adults and contrasting with males, in whom the distal portion forms the tunica vaginalis in nearly all adults, while proximal patency to the peritoneum persists in approximately 20% of adults.^[1]^[13] This high rate of regression underscores its vestigial status in female anatomy.^[18]

Clinical significance

Patent processus vaginalis

A patent processus vaginalis refers to the incomplete obliteration of the processus vaginalis, a peritoneal extension that fails to close proximally after testicular descent, resulting in persistent communication between the peritoneal cavity and the scrotum in males or the canal of Nuck in females.^[1] This condition arises from the normal embryologic process where the processus vaginalis typically obliterates postnatally, but persistence allows potential passage of peritoneal fluid or contents through the inguinal canal.^[13] The incidence of patent processus vaginalis is estimated at 15-30% in adult males and 5-10% in adult females, with higher rates in newborns (80-94%) that decrease with age; many cases remain asymptomatic throughout life, discovered incidentally at autopsy or surgery.^[20] Premature infants face an elevated risk, with up to 30% developing related inguinal hernias due to delayed or incomplete closure.^[21] Patency is classified into complete and partial types: complete patency involves full openness from the deep inguinal ring to the scrotum or labia, enabling bidirectional flow; partial patency occurs when the distal portion closes but the proximal segment near the internal ring remains open, often leading to isolated fluid accumulation without full herniation.^[1] Diagnosis typically relies on clinical examination revealing reducible scrotal or groin swellings that fluctuate with activity or position, supplemented by ultrasound imaging that demonstrates fluid tracking from the peritoneal cavity through the patent channel into the scrotum or inguinal region.^[22] In ambiguous cases, especially in infants, ultrasound provides high accuracy (up to 95%) in confirming patency by visualizing peristalsis of bowel loops or fluid movement across the internal ring.^[23]

Associated conditions

Abnormalities of the vaginal process, particularly its failure to fully obliterate, are primarily associated with indirect inguinal hernias and hydroceles in males. An indirect inguinal hernia occurs when abdominal contents, such as bowel or omentum, protrude through a patent processus vaginalis into the scrotum or inguinal canal, representing over 90% of all pediatric inguinal hernias.^[1]^[24] This condition arises due to the persistence of the peritoneal connection, allowing herniation of viscera.^[25] Hydroceles, another common sequela, involve the accumulation of serous fluid within the tunica vaginalis or along a patent processus vaginalis, leading to scrotal swelling.^[13] They are classified as communicating (due to ongoing fluid passage through a patent process) or non-communicating (isolated fluid retention after partial closure). In infants, communicating hydroceles often resolve spontaneously, with approximately 90% regressing by age 2 years as the processus obliterates naturally.^[13] Non-communicating hydroceles, however, may persist and require intervention if symptomatic or enlarging.^[13] Other related conditions include encysted hydroceles, which form when the processus vaginalis closes at both the internal ring and near the testis, trapping fluid in a isolated segment of the spermatic cord, and spermatoceles, cystic collections of fluid and spermatozoa within the epididymis that can mimic hydroceles but are not directly tied to patency.^[26] In females, a rare equivalent is hydrocele of the canal of Nuck, resulting from incomplete obliteration of this homologous peritoneal extension, presenting as inguinal or labial swelling predominantly in young girls.^[20] Management of these conditions emphasizes minimizing complications, particularly incarceration, where herniated contents become trapped, occurring in 3-6% of untreated pediatric inguinal hernias with higher rates (up to 30%) in young infants.^[27] For asymptomatic hydroceles in infants, observation is standard, allowing spontaneous resolution, while persistent or symptomatic cases warrant surgical excision.^[13] Indirect inguinal hernias necessitate prompt surgical repair via herniorrhaphy to ligate the patent processus and close the defect, typically performed laparoscopically or openly in children to prevent recurrence and incarceration.^[24]

References

[1]
Persistence of the processus vaginalis and its related disorders - PMC
Feb 20, 2020 · The processus vaginalis is a blind‐ended evagination of the abdominal wall that develops during fetal life and typically undergoes obliteration ...
[2]
Animation - Descent of the testis
The processus vaginalis is a diverticulum of the peritoneal cavity that extends into the scrotum or labium majus. In the male, the gubernaculum shortens, ...
[3]
Anatomy, Abdomen and Pelvis: Testes - StatPearls - NCBI Bookshelf
During development the inguinal canal contains the processus vaginalis, which is a structure that develops from the peritoneum. It allows the testes to ...
[4]
Anatomy, Abdomen and Pelvis: Inguinal Region (Inguinal Canal)
With the descent of the testicles, a peritoneal outpouching called the processus vaginalis follows the testicles to the scrotum. Following the descent of the ...
[5]
Anatomy and pathology of the canal of Nuck - PubMed
Feb 7, 2018 · The canal of Nuck is the female equivalent of the processus vaginalis in the male but is less well known than its male counterpart.
[6]
[PDF] US of the Inguinal Canal: Com- prehensive Review of Pathologic ...
The processus vaginalis (PV), an evagina- tion of the parietal peritoneum, and the guber- naculum, a fibromascular ligament, have major roles in the ...
[7]
Inguinal Region Anatomy - Medscape Reference
Apr 21, 2025 · The processus vaginalis, an outpouching of peritoneum, forms during this phase to guide the testes. Inguinoscrotal Migration:
[8]
Inguinal canal: Anatomy, contents and hernias | Kenhub
The flask-shaped processus vaginalis is an evaginated portion of peritoneum that bulges through the anterior abdominal wall.Missing: composition | Show results with:composition
[9]
Embryology, Testicle - StatPearls - NCBI Bookshelf - NIH
Apr 24, 2023 · Obliteration of the processus vaginalis occurs typically by the third trimester through programmed cell death of smooth muscle cells.
[10]
Testicular development and descent | Radiology Reference Article
Sep 7, 2025 · By the 8th week of development, the testis is attached at its caudal pole to the gubernaculum ... processus vaginalis. This invaginates through ...
[11]
The role of the gubernaculum in the descent ... - PubMed Central - NIH
The processus vaginalis develops as a diverticulum of the peritoneal membrane inside the gubernaculum, dividing it into three parts. The outer rim of mesenchyme ...
[12]
Hydrocele - StatPearls - NCBI Bookshelf
Jul 3, 2023 · Primary Hydrocele: The processus vaginalis of the spermatic cord fuses at term or within 1-2 years of birth, thus obliterating the ...
[13]
https://www.ncbi.nlm.nih.gov/books/NBK559125/
[14]
Tunica vaginalis (testis) | Radiology Reference Article
May 7, 2018 · The tunica vaginalis (TV) represents the investing serosal covering of the testis. It forms as the embryological testis descends and passes out through the ...
[15]
Tunica Vaginalis - an overview | ScienceDirect Topics
The outer layer of peritoneum, the processus vaginalis (tunica vaginalis reflexa), is reflected on to the testis to form the serous outer layer of that organ, ...
[16]
https://www.sciencedirect.com/topics/immunology-and-microbiology/tunica-vaginalis
[17]
The Inguinal Canal - Boundaries - Contents - TeachMeAnatomy
### Summary of Processus Vaginalis in Females (Normal Adult Anatomy)
[18]
Normal Vulvovaginal, Perineal, and Pelvic Anatomy with ...
The Colles fascia is inferiorly attached to the ischiopubic rami, posteriorly to the urogenital diaphragm, but lacks anterior attachment. ... processus vaginalis ...
[19]
Hydrocele of the canal of Nuck | Radiology Reference Article
Nov 5, 2024 · As many as 30% of adults are discovered to have a patent processus vaginalis at autopsy.Missing: prevalence | Show results with:prevalence
[20]
Contralateral patent processus vaginalis repair in boys - Nature
Jul 15, 2022 · The processus vaginalis is formed in the embryo and closes during development but is not yet fully obliterated at birth. CPPV may develop into ...
[21]
Accuracy of ultrasonography in predicting contralateral patent ...
May 8, 2024 · The study aims to evaluate the usefulness of preoperative ultrasonography (US) at the internal inguinal ring level in predicting contralateral patent processus ...
[22]
Preoperative sonographic evaluation is a useful method of detecting ...
Preoperative sonographic evaluation is a useful method of detecting contralateral patent processus vaginalis in pediatric patients with unilateral inguinal ...
[23]
Pediatric Hernias: Practice Essentials, Pathophysiology, Etiology
Dec 28, 2023 · The processus vaginalis is an outpouching of peritoneum attached to ... week, invade the genital ridges, which lie on the medial aspect ...
[24]
Indirect Inguinal Hernia
Inguinal hernias in children almost always result from a persistence of the patent processus vaginalis. Like hydroceles, peritoneal fluid can move into the ...
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Persistence of the processus vaginalis and its related disorders
Feb 20, 2020 · Fluid is seen within the patent processus vaginalis (star) entering at the deep inguinal ring (arrow) and extending distally. (b) Fluid shown ...
[26]
Assessment and Management of Inguinal Hernias in Children
Jun 26, 2023 · The risk of an incarcerated inguinal hernia in children is estimated to be 4%, with the highest risk (8%) noted in infants; consequently, they ...