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Sputum

Sputum is a viscous mixture of saliva, mucus, and cellular debris expectorated from the lower respiratory tract through coughing, often as a response to infection, inflammation, or irritation of the airways.^[1] It serves a physiological role in protecting the lungs by trapping and expelling pathogens, dust, and other irritants while humidifying inhaled air.^[2] The composition of sputum primarily consists of water (approximately 95%), along with electrolytes, glucose, mucus glycoproteins (mucins), proteins, lipids, and cellular components such as leukocytes, macrophages, bronchial epithelial cells, and potentially microorganisms or blood if infection or injury is present.^[3] These elements can vary based on the underlying condition; for instance, in bacterial infections, sputum may appear purulent (containing pus) and change color from clear or white to yellow, green, or rust-colored, indicating the presence of neutrophils and bacterial load.^[4] Clinically, sputum analysis is a cornerstone for diagnosing respiratory diseases, including pneumonia, bronchitis, tuberculosis, and chronic obstructive pulmonary disease (COPD), through methods such as Gram staining, culture, cytology, and molecular testing to identify pathogens, assess inflammation, and guide treatment.^[1] The quality and quantity of sputum produced can also reflect disease severity; excessive or tenacious sputum in conditions like cystic fibrosis or COVID-19 may impair airway clearance and oxygenation, necessitating interventions such as chest physiotherapy or mucolytics.^[5]

Physiology

Production Mechanisms

Sputum is produced in the lower respiratory tract through the secretion of mucus by specialized epithelial cells, forming a protective layer that traps inhaled particles and facilitates their removal via mucociliary clearance. This process involves coordinated ciliary beating on the surface of ciliated epithelial cells, which propels the low-viscosity mucus gel toward the oropharynx for expectoration or swallowing.^[6] Goblet cells, located on the airway surface epithelium, primarily secrete gel-forming mucins such as MUC5AC, while submucosal glands, embedded beneath the epithelium, produce MUC5B and contribute serous components that hydrate the mucus layer.^[7] These secretions originate mainly in the trachea and bronchi, where submucosal glands are abundant, and to a lesser extent in bronchioles, which contain fewer glands but still feature goblet cells; this distinguishes sputum from saliva, which is produced by oral glands, and nasal discharge, derived from upper airway mucosa.^[3]^[8] The production and secretion of mucus are regulated by the autonomic nervous system, with parasympathetic innervation playing a dominant role in stimulating glandular output through muscarinic receptor activation on mucous and serous cells.^[9] Parasympathetic stimulation, mediated by acetylcholine release from nerve endings, increases both the volume and viscosity of secretions in submucosal glands, enhancing baseline mucus production during normal respiration.^[10] Sympathetic influences are less prominent but can modulate secretion indirectly via vascular effects.^[11] Under stimulated conditions, sputum production increases due to irritation from pathogens, allergens, or environmental irritants, which trigger inflammatory cascades leading to goblet cell hyperplasia and metaplasia.^[12] For instance, exposure to bacterial pathogens like Pseudomonas aeruginosa or allergens such as those in asthma induces epidermal growth factor receptor (EGFR) signaling, promoting differentiation of club cells into mucus-producing goblet cells and elevating mucin gene expression.^[13]^[14] This inflammatory response, involving cytokines like IL-13 and TNF-α, results in submucosal gland hypertrophy and accelerated secretion, overwhelming normal mucociliary clearance and necessitating cough-mediated expulsion of excess sputum.^[15]

Composition and Normal Properties

Sputum in healthy individuals is a hydrogel primarily composed of water, which constitutes approximately 95–97% of its total volume, along with salts (about 0.9%), globular proteins (∼1.1%), lipids, ions, and high-molecular-weight mucin polymers (∼0.5%).^[16] The key mucin glycoproteins are MUC5AC and MUC5B, secreted by goblet cells and submucosal glands in the airways, which form entangled polymeric networks that impart the gel-like consistency essential for mucociliary clearance.^[17]^[18] Additional components include low levels of extracellular DNA derived from neutrophils and epithelial cells, as well as antimicrobial proteins and cellular debris that contribute to innate defense without dominating the matrix.^[19]^[20] The cellular fraction of normal sputum is sparse, with total non-squamous cell counts typically ranging from 2.4 × 10⁶ to 4.1 × 10⁶ cells per gram (median viability ∼70%).^[21] Macrophages predominate, accounting for 50–80% of the differential cell count, reflecting their role in phagocytosis and surveillance in the lower airways.^[22] Neutrophils comprise 20–50%, while eosinophils and lymphocytes each represent less than 2%, and bronchial epithelial cells are minimal (∼1–5%), indicating baseline homeostasis rather than inflammation.^[23]^[24] Normal sputum exhibits a pH of approximately 7.0–7.2, maintained by airway epithelial ion transport mechanisms that regulate bicarbonate and proton secretion.^[25] Viscosity arises from the hydration state of the mucus gel and the physical entanglement of mucin polymers, which creates a viscoelastic network with low shear viscosity (comparable to water at high flow rates) but high elasticity at rest, facilitating ciliary beating and pathogen trapping.^[6]^[26] In contrast to saliva, which contains lower mucin concentrations (primarily MUC5B and MUC7) and digestive enzymes like amylase for oral processing, sputum features elevated levels of airway-specific gel-forming mucins (MUC5AC and MUC5B) and lacks such enzymatic activity, emphasizing its role in respiratory protection over lubrication and digestion.^[27]^[28]

Clinical Examination

Collection Techniques

Sputum collection techniques are essential for obtaining representative samples from the lower respiratory tract for diagnostic evaluation, distinguishing them from upper airway secretions like saliva. The primary methods include spontaneous expectoration, induced production, and invasive procedures, each selected based on patient ability and clinical context. Proper preparation and precautions ensure sample integrity and minimize contamination. Spontaneous expectoration is the simplest and preferred initial approach for patients capable of producing sputum naturally. Patients are advised to collect samples in the morning, as overnight accumulation in the lungs yields more concentrated material from the lower airways. To facilitate production, individuals should maintain hydration by drinking plenty of fluids the day prior and may perform chest physiotherapy techniques, such as postural drainage or percussion, to loosen secretions. Oral hygiene is emphasized, with patients rinsing their mouth with plain water just before collection to reduce salivary contamination from oral flora or residual food particles. Instructions stress deep coughing from the chest rather than clearing the throat, producing a thick, viscous sample indicative of lower respiratory origin, and expectorating directly into a sterile, wide-mouthed container without touching the inside. For patients unable to spontaneously expectorate adequate sputum, induced sputum collection stimulates production through controlled inhalation. This method begins with pre-treatment using a short-acting bronchodilator, such as inhaled salbutamol, to prevent bronchospasm triggered by the irritant. Hypertonic saline (typically 3-5%) is then nebulized and inhaled for 10-15 minutes per dose, escalating concentrations if needed, while the patient performs deep breathing or coughing to mobilize secretions. Adjunctive measures like chest percussion or vibration may enhance yield. The procedure is conducted in a supervised setting with monitoring for adverse effects, and the sample is collected into a sterile container immediately following inhalation. Invasive techniques are reserved for cases where non-invasive methods fail or when targeting specific lower airway sites is necessary, such as in critically ill or intubated patients. Bronchoscopy with bronchoalveolar lavage (BAL) involves inserting a flexible bronchoscope through the mouth or nose under sedation to instill and aspirate saline into lung segments, yielding high-quality cellular and microbial samples from the alveoli. For mechanically ventilated patients, endotracheal aspiration uses a sterile catheter passed through the endotracheal tube to suction secretions directly from the trachea, providing rapid access without additional instrumentation. Key precautions across all methods focus on avoiding contamination and preserving sample viability. Patients receive clear guidance on deep coughing to ensure lower tract origin, and containers are handled aseptically to prevent external microbial introduction. Collected sputum should be processed as soon as possible. If immediate processing is not possible, it should be refrigerated at 4°C and analyzed within 24 hours to maintain cellular integrity and diagnostic accuracy, as longer delays can degrade components or allow overgrowth.^[1]

Macroscopic Evaluation

Macroscopic evaluation of sputum involves the initial visual and sensory assessment of its physical properties, such as color, consistency, volume, and odor, performed at the bedside or in the laboratory to inform subsequent diagnostic steps.^[3] This examination helps distinguish normal from abnormal samples without requiring advanced equipment.^[1] Color provides key insights into sputum composition. Clear or white sputum is typically mucoid and indicative of normal mucus production.^[3] Yellow or green hues suggest purulent material, often due to increased cellular content.^[3] Rust or brown discoloration may result from old blood or specific inflammatory processes.^[29] Pink or red sputum indicates fresh blood.^[3] Consistency describes the texture and viscosity of the sample. Serous sputum is thin and watery, while mucoid sputum appears gel-like and viscous.^[3] Purulent sputum is thick, opaque, and tenacious.^[3] In certain cases, sputum may exhibit a caseous, cheesy appearance.^[30] Normal daily sputum volume is less than 30 mL.^[31] Increased production, exceeding 100 mL per day, can occur in conditions like bronchitis.^[32] Odor assessment can reveal underlying processes. Foul-smelling sputum is associated with anaerobic bacterial involvement.^[33] A sweet or fruity odor may indicate Pseudomonas species.^[34] The presence of blood in sputum, known as hemoptysis, is evaluated by its pattern and amount. Streaking refers to small amounts of blood mixed with sputum, whereas massive hemoptysis involves more than 200 mL of blood expectorated within 24 hours.^[35] Foreign materials, such as particles or debris, may also be visible and noted during inspection.^[3]

Diagnostic Analysis

Microscopic Examination

Microscopic examination of sputum involves preparing smears or mounts to visualize cellular components, microorganisms, and structures under a light microscope, often guided by the sample's macroscopic appearance such as purulence, which may indicate increased inflammatory cells or bacteria.^[1] Gram staining is a primary technique used to differentiate bacteria in sputum smears by their cell wall properties, revealing Gram-positive organisms (appearing purple, such as cocci in clusters suggesting staphylococci or chains indicating streptococci) from Gram-negative ones (appearing pink, like rods in pneumonia cases).^[36] The presence of squamous epithelial cells in the smear, typically exceeding 25 per low-power field (LPF, 100x magnification), signals significant oral contamination and poor sample quality, warranting rejection or recollection.^[37] Conversely, a high number of polymorphonuclear leukocytes (PMNs, or neutrophils) greater than 25 per LPF supports sample adequacy from lower respiratory tract origins.^[38] Cytological evaluation entails preparing a thin smear stained with agents like Wright-Giemsa or Diff-Quik for differential cell counting, quantifying percentages of key cell types including neutrophils, eosinophils, macrophages, and lymphocytes across at least 400 non-squamous cells.^[1] Neutrophils exceeding 70% of the total non-epithelial cells often indicate acute inflammation, while eosinophils surpassing 5% suggest allergic or eosinophilic processes.^[39]^[40] In such cases, characteristic structures like Curschmann spirals—coiled mucoid casts of desquamated epithelium—and Charcot-Leyden crystals—elongated, bipyramidal eosinophil-derived crystals—may be observed, providing insights into airway pathology.^[41] Acid-fast staining, particularly the Ziehl-Neelsen method, employs carbolfuchsin dye heated with phenol to penetrate waxy cell walls, followed by acid-alcohol decolorization; acid-fast mycobacteria retain the red color against a blue counterstain background, enabling detection of organisms like Mycobacterium tuberculosis at 1000x oil immersion.^[42] This technique is essential for identifying mycobacterial infections in sputum, with positive results showing rod-shaped bacilli resistant to decolorization. Wet mount preparations involve placing a drop of sputum in saline or iodine on a slide under a coverslip for direct observation of motile elements without fixation, useful for detecting parasites such as Strongyloides stercoralis larvae or fungal hyphae in cases of suspected disseminated infection.^[43] Quantitation in these mounts focuses on structures per LPF, with overall sample rejection criteria including fewer than 10 PMNs per LPF or excessive squamous cells, ensuring reliable microscopic interpretation.^[38]

Microbiological and Molecular Testing

Microbiological testing of sputum primarily involves culture-based methods to isolate and identify pathogens. Sputum samples are inoculated onto selective media to promote the growth of specific microorganisms. For bacterial pathogens, aerobic cultures are performed on blood agar or chocolate agar, while anaerobic cultures use media such as Brucella agar supplemented with vitamin K and hemin to detect obligate anaerobes like Bacteroides species. Fungal cultures employ Sabouraud dextrose agar, which supports the growth of yeasts and molds such as Candida and Aspergillus. Mycobacterial cultures require specialized media like Lowenstein-Jensen, an egg-based solid medium, where Mycobacterium tuberculosis typically requires 3 to 8 weeks of incubation at 37°C for visible colony formation.^[44]^[45]^[1]^[46] Once pathogens are isolated, antibiotic susceptibility testing determines treatment options. The disk diffusion method, also known as the Kirby-Bauer test, is widely used to assess susceptibility of common respiratory bacteria like Streptococcus pneumoniae to antibiotics such as penicillin. In this technique, antibiotic-impregnated disks are placed on agar plates inoculated with the isolate, and zones of inhibition are measured after overnight incubation to classify the organism as susceptible, intermediate, or resistant according to Clinical and Laboratory Standards Institute guidelines. This phenotypic approach provides actionable data for guiding antimicrobial therapy in sputum-derived isolates.^[44]^[47] Molecular methods offer rapid detection without relying on culture growth. Polymerase chain reaction (PCR)-based assays, such as the Xpert MTB/RIF Ultra system, enable simultaneous identification of Mycobacterium tuberculosis complex DNA and rifampin resistance mutations in sputum within 2 hours, with high sensitivity for smear-positive cases.^[48]^[49] Multiplex PCR panels, like the FilmArray Pneumonia Panel, detect multiple bacterial, viral, and atypical pathogens—including influenza, respiratory syncytial virus (RSV), and Legionella—in a single sputum or respiratory sample, providing results in about 1 hour and aiding in the diagnosis of community-acquired pneumonia. These nucleic acid amplification tests (NAATs) are particularly valuable for timely initiation of targeted therapy.^[50]^[51]^[52] Emerging technologies, such as CRISPR-based assays, have shown promise for direct, rapid detection of tuberculosis in sputum with high accuracy as of 2025.^[53] Next-generation sequencing (NGS) extends detection to unculturable organisms and complex polymicrobial infections in sputum. Metagenomic NGS analyzes total DNA from samples to identify a broad range of bacteria, viruses, fungi, and parasites without prior knowledge of the pathogen, revealing fastidious or novel agents that evade traditional cultures. Targeted NGS, focusing on 16S rRNA for bacteria, has demonstrated utility in characterizing polymicrobial respiratory communities, such as in ventilator-associated pneumonia, where multiple species contribute to infection. This approach enhances diagnostic yield in cases with negative cultures, though it requires bioinformatics for interpretation.^[54]^[55]^[56] Despite their utility, these tests face limitations. Contamination from oropharyngeal flora can lead to false-positive results in cultures, necessitating quality assessment via Gram stain to ensure lower respiratory origin. False negatives may occur due to prior antibiotic exposure, which inhibits bacterial growth, or low pathogen burden in early infection stages. Molecular methods like PCR can miss low-load infections or non-viable DNA, while NGS is hindered by host DNA overload and high costs.^[57]^[58]^[59]

Pathological Associations

In Respiratory Infections

In respiratory infections, sputum serves as a key diagnostic specimen, with its macroscopic appearance and microbiological content providing clues to the underlying pathogen. Purulent sputum, often yellow or green due to high neutrophil counts, is characteristic of bacterial etiologies, while clearer or scant production suggests viral causes. Microscopic and culture-based analyses further differentiate infectious agents, guiding targeted therapy. Bacterial pneumonia commonly presents with purulent yellow sputum, reflecting intense neutrophilic response to infection. In cases caused by Streptococcus pneumoniae, Gram staining of sputum reveals Gram-positive diplococci, and culture confirmation identifies the organism and its antibiotic susceptibility.^[60]^[61] Viral respiratory infections typically yield scant, clear sputum with minimal purulence, as inflammation is predominantly lymphocytic rather than neutrophilic. Polymerase chain reaction (PCR) assays on sputum effectively detect rhinovirus in common colds, offering higher sensitivity than nasopharyngeal swabs in adults.^[62] For SARS-CoV-2 in COVID-19 cases, sputum PCR is effective and can yield significantly higher detection rates than nasopharyngeal swabs.^[63] Tuberculosis often manifests with bloody or caseous sputum, the latter arising from necrotic material in cavitary lung lesions. Diagnostic evaluation includes acid-fast bacillus (AFB) smears showing rod-shaped bacilli and confirmatory cultures on specialized media, with induced sputum employed when spontaneous production is inadequate.^[64] Fungal respiratory infections in immunocompromised hosts, such as mucormycosis, feature sputum containing broad, branching non-septate hyphae visible on histopathology, while aspergillosis displays acute-angle branching septate hyphae. These findings, often from sputum cytology, prompt urgent antifungal therapy.^[65]^[66] Atypical pathogens require specialized diagnostics beyond routine sputum culture. For Legionella pneumophila, urine antigen testing is rapid and sensitive, supplemented by sputum culture on buffered charcoal yeast extract (BCYE) agar; Mycoplasma pneumoniae is identified via PCR on respiratory specimens or serologic detection of cold agglutinins.^[67]^[68] Recent advances in sputum analysis include metagenomic next-generation sequencing (mNGS) for community-acquired pneumonia, which detects a broader range of pathogens with greater sensitivity than conventional methods, aiding in cases where standard cultures fail.^[69]

In Chronic Lung Diseases

In chronic obstructive pulmonary disease (COPD), sputum production is a hallmark symptom, often presenting as increased mucoid sputum during stable phases due to chronic airway inflammation.^[70] Neutrophils predominate in the sputum cellular profile, reflecting ongoing neutrophilic inflammation that contributes to tissue damage and airflow limitation.^[71] During exacerbations, sputum volume typically increases, and a shift to purulent sputum—often indicated by yellow or green coloration—signals bacterial involvement, with up to 84% of such cases yielding positive bacterial cultures compared to 38% in mucoid sputum samples.^[72] This purulence correlates with elevated neutrophil counts and markers like C-reactive protein, guiding antibiotic therapy to mitigate exacerbation severity.^[73] In asthma, particularly eosinophilic phenotypes, sputum analysis reveals a predominance of eosinophils, which drive allergic airway inflammation and are detectable in up to 50% of patients despite corticosteroid treatment.^[74] Induced sputum samples, obtained via hypertonic saline inhalation, are commonly used to quantify this eosinophilia and correlate strongly with fractional exhaled nitric oxide (FeNO) levels, especially in mild-to-moderate disease, aiding in phenotyping and treatment selection.^[75] Microscopic examination may uncover Charcot-Leyden crystals, needle-like structures formed from eosinophil lysophospholipase, which are indicative of active eosinophilic degranulation and often present in allergic asthma exacerbations.^[76] These findings help differentiate eosinophilic from non-eosinophilic asthma, influencing responses to targeted biologics. Cystic fibrosis (CF) is characterized by thick, purulent sputum resulting from defective mucociliary clearance and chronic endobronchial infection, leading to viscous mucus that impairs lung function.^[77] Pseudomonas aeruginosa dominates the sputum microbiome in advanced CF, often as mucoid strains that produce alginate, fostering persistent colonization in the airways.^[78] These mucoid variants form biofilms within the sputum and airway surfaces, enhancing bacterial resistance to antibiotics and host defenses, which accelerates pulmonary decline and necessitates specialized antimicrobial strategies.^[79] In interstitial lung diseases (ILDs), such as idiopathic pulmonary fibrosis (IPF), sputum production is typically scant due to the predominant fibrotic and restrictive pathology rather than productive cough.^[80] When available, sputum cytology plays a diagnostic role in detecting associated malignancies, with adenocarcinoma cells identifiable in samples from IPF patients at risk for lung cancer, highlighting the need for vigilant screening in this population.^[81] Bronchiectasis features copious volumes of foul-smelling, purulent sputum, often exceeding daily norms and resulting from irreversible airway dilation and impaired clearance.^[3] Sputum cultures commonly reveal mixed flora, including Haemophilus influenzae, Streptococcus pneumoniae, and Pseudomonas species, reflecting polymicrobial colonization that drives recurrent inflammation and structural damage.^[82] Sputum eosinophil counts serve as a key monitoring tool in severe asthma, with levels exceeding 3% predicting poor control and guiding corticosteroid adjustments to achieve suppression below this threshold, thereby reducing exacerbation risk.^[83] In biologic-treated patients, normalization to under 3% eosinophils correlates with improved outcomes, including fewer asthma attacks and better lung function.^[84]

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Jul 12, 2017 · Our study reports that, out of 94 smear-positive cases, 64% were both GeneXpert and culture negative and identified nontuberculous mycobacteria ...
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Typical pneumonia refers to pneumonia primarily caused by Streptococcus pneumoniae ... sputum characteristics, such as: S pneumoniae: Rust-colored [73].
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Diagnosis of Pneumococcal Pneumonia: Current Pitfalls and the ...
Sputum Gram stains and culture are the first diagnostic step for identifying pneumococcal pneumonia and provide information on antibiotic susceptibility.
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Comparison of sputum and nasopharyngeal swabs for detection of ...
Sputum is the representative of lower respiratory secretions and is the most widely accepted specimen for the diagnosis of bacterial respiratory infection.Sputum · Results · DiscussionMissing: composition | Show results with:composition
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Using a microscope, laboratory personnel look for AFB. If AFB are found on a sputum smear, it often indicates TB disease (smear positive).Missing: bloody caseous
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May 8, 2024 · Fungal hyphae occlude the lumen of the pulmonary artery with associated infarcted area (Semin Diagn Pathol 2017;34:530). Microscopic ...<|separator|>
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The aspergilloma (fungus ball) is composed of fungal hyphae, inflammatory cells, fibrin, mucus, and tissue debris.
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Sep 10, 2024 · Molecular-based diagnosis of nasopharyngeal swabs, throat swabs or sputum can be performed. Serology can be used to confirm the diagnosis.
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Culture of sputum remains the reference standard and allows the detection of all Legionella species. However, it is slow (up to seven days), labour ...
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Sep 18, 2024 · This study aims to determine the clinical use of mNGS in CAP, HAP, and VAP, compared with traditional microbiological methods.
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Oct 9, 2017 · Sputum colour is associated with neutrophilic inflammation in chronic bronchitis and chronic obstructive pulmonary disease (COPD).
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Sputum eosinophil count is increased in symptomatic individuals with asthma, and elevated eosinophils can be found in 50% of corticosteroid-treated patients, ...
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FeNO levels are correlated with peripheral blood eosinophils [Zietkowski et al. 2006] and sputum eosinophils particularly in mild-to-moderate disease whereas ...
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Targeting polyelectrolyte networks in purulent body fluids to ...
Major pathogens responsible for infections in CF patients are Staphylococcus aureus and Haemophilus influenzae in early stages of disease and Pseudomonas ...Material And Methods · Results · Dnase I And P-Asp Enhance...
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The mucoid biofilm mode of growth of Pseudomonas aeruginosa (P. aeruginosa) in the lungs of cystic fibrosis patients makes eradication of infections with ...
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The ΔF508-CFTR mutation results in increased biofilm formation by ...
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Interstitial lung diseases—can pathologists arrive at an etiology ...
Interstitial lung diseases (ILD) are characterized by a diffuse infiltration of both lungs, usually evaluated by high-resolution computed tomography (HRCT) scan ...Missing: sputum scant
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Nevertheless there is still a role for sputum cytology in the diagnosis of lung cancer with reported sensitivities of around 42 % for one specimen, 82 % for ...
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Submission of nasal swabs for culture yields mixed flora and the results are impossible to interpret, with the exception noted previously. Organisms cultured ...
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Evaluation of sputum eosinophil count as a predictor of treatment ...
A previous study has indicated that maintaining sputum eosinophils <3% can reduce the frequency of asthma exacerbations [11]. In our analysis, reductions in ...
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Benralizumab in severe eosinophilic asthma in real life - NIH
Nov 27, 2023 · 85% of patients receiving benralizumab for 6 months normalised their sputum eosinophil counts to <3% and this figure reached 100% after 30 ...Secondary Outcomes · Effect On Asthma Control And... · Effect On Lung Function