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5-HT6 receptor

The 5-HT6 receptor, also known as the serotonin 6 receptor, is a subtype of the G protein-coupled receptor (GPCR) family that binds the neurotransmitter serotonin (5-hydroxytryptamine; 5-HT) as its endogenous ligand.^[1] Encoded by the HTR6 gene on human chromosome 1p35-p36, it consists of 440 amino acids forming seven transmembrane domains typical of class A GPCRs, with no known isoforms.^[1] Unlike many serotonin receptors that inhibit adenylyl cyclase, the 5-HT6 receptor couples positively to Gs proteins, stimulating adenylyl cyclase to increase intracellular cyclic AMP (cAMP) levels and exhibiting notable constitutive (ligand-independent) activity mediated by specific residues like T280^{6.47} and N312^{7.45}.^[1]^[2] Primarily expressed in the central nervous system—with high densities in the striatum, nucleus accumbens, olfactory tubercle, hippocampus, and cortex, but with very low or undetectable levels in peripheral tissues—the 5-HT6 receptor modulates key neurotransmitter systems including cholinergic, glutamatergic, and GABAergic pathways.^[1] It interacts with intracellular proteins such as Fyn kinase and Jab1/CSN5, influencing neuronal signaling beyond cAMP production.^[1] Pharmacologically, selective agonists like EMDT and WAY-181187 mimic serotonin's effects, while antagonists such as SB-271046, SB-399885, and idalopirdine block receptor activity, often enhancing cognition in preclinical models by facilitating acetylcholine and glutamate release.^[1] Over 20 antagonists have been identified, with several advancing to clinical trials.^[1] The receptor's roles extend to learning, memory consolidation, and emotional regulation, with antagonism linked to procognitive effects in animal studies of scopolamine- or NMDA-induced deficits.^[1] Therapeutically, 5-HT6 antagonists show promise for neuropsychiatric disorders; for instance, they have been investigated for Alzheimer's disease (AD) to improve cognition and behavioral symptoms like agitation, with drugs like idalopirdine reaching phase III trials but failing to demonstrate efficacy, and masupirdine currently in phase III for AD-related agitation as of 2025.^[3]^[4] Emerging evidence also supports potential in schizophrenia, anxiety, and depression, where receptor modulation may address negative symptoms and cognitive impairments without the side effects of dopamine-focused therapies.^[1]^[5]

Molecular Structure

Receptor Architecture

The 5-HT6 receptor belongs to the class A subfamily of G protein-coupled receptors (GPCRs), exhibiting the canonical architecture of seven α-helical transmembrane domains (TM1–TM7) that span the lipid bilayer. These helices are interconnected by three extracellular loops (ECL1–ECL3) and three intracellular loops (ICL1–ICL3), with an extracellular N-terminal domain and an intracellular C-terminal tail. This topological arrangement forms a compact bundle approximately 35–40 Å in height, typical of aminergic class A GPCRs, enabling ligand access from the extracellular side and intracellular signaling interactions.^[2]^[6]^[7] The orthosteric binding pocket resides deep within the transmembrane core, primarily lined by residues from TM3, TM5, TM6, and TM7, as well as contributions from ECL2. A hallmark feature is the conserved aspartate residue Asp^{3.32} (using Ballesteros-Weinstein numbering), which engages in a salt bridge with the protonated amine group of serotonin, anchoring the ligand in the pocket. Additional stabilizing interactions involve aromatic residues such as Phe^{6.52} for π-stacking and polar residues like Ser^{5.43} and Thr^{5.46} for hydrogen bonding.^[2]^[7]^[8] Conserved motifs critical to the receptor's function include the DRY sequence (Asp^{3.49}-Arg^{3.50}-Tyr^{3.51}) at the cytoplasmic terminus of TM3, which stabilizes the inactive state and facilitates conformational changes upon activation. The 5-HT6 receptor displays low sequence homology with other serotonin receptors, sharing less than 35% amino acid identity overall—for instance, approximately 30–35% with the 5-HT_{1A} subtype—distinguishing it structurally while retaining core GPCR features.^[7]^[9]^[10]

Structural Determinants of Activity

The cryo-electron microscopy (cryo-EM) structure of the human 5-HT6 receptor (5-HT6R) bound to serotonin in complex with the heterotrimeric Gs protein has been determined at 3.0 Å resolution, providing detailed insights into activation mechanisms.^[2] Upon ligand binding, transmembrane helix 6 (TM6) undergoes an outward displacement by 7.9 Å at its intracellular end, a hallmark conformational change that opens the G-protein binding interface and promotes Gs engagement.^[2] This structure highlights the receptor's active state, with TM5 tilting toward TM6 to stabilize the open intracellular cavity.^[2] A defining feature of the 5-HT6R is its elevated constitutive activity, which persists in the absence of ligand and drives basal Gs signaling.^[11] Two primary structural determinants underpin this property: an extended and flexible intracellular loop 3 (ICL3), which spans approximately 50 residues and facilitates adoption of an active-like conformation by reducing steric constraints on the intracellular side; and an interhelical hydrogen bond between Thr280^{6.47} in TM6 and Asn312^{7.45} in TM7, which locks the receptor in a partially activated pose and eliminates inhibitory sodium ion allosteric modulation observed in many other GPCRs.^[2] These elements collectively bias the 5-HT6R toward higher basal signaling compared to ligand-dependent activation in related receptors.^[2] In comparison to other serotonin receptors, the 5-HT6R possesses an enlarged orthosteric binding pocket, formed by inward shifts of TM7 and helix 8, enabling preferential accommodation of bulky, selective ligands such as atypical antipsychotics.^[12] This expanded pocket is further delineated by unique residues, including Thr^{5.46} (position 5.46 in Ballesteros-Weinstein numbering), which forms a hydrogen bond with the indole nitrogen of serotonin, contrasting with alanine or other non-bonding residues at equivalent positions in 5-HT4R and 5-HT7R.^[12] Additionally, the extended length of TM5 (5.7 residues longer than in Gi/o-coupled 5-HT subtypes) contributes to this selectivity by altering the pocket's depth and ligand orientation.^[12] The second extracellular loop (ECL2) plays a critical role in modulating agonist interactions by acting as a dynamic lid over the binding pocket entrance in the 5-HT6R.^[12] Structural comparisons reveal that ECL2 in 5-HT6R adopts a distinct conformation facilitating ligand entry, while conserved residues like those in the β-hairpin motif interact with TM3 and TM5 to gate access.^[6] Mutational analyses of analogous ECL2 positions in serotonin receptors, including substitutions at residues interfacing with the orthosteric site, demonstrate reduced agonist efficacy and potency for serotonin and synthetic agonists, with effects attributable to disrupted hydrogen bonding networks that alter pocket stability and ligand dwell time.^[6]

Gene and Tissue Distribution

Genetics and Chromosomal Location

The HTR6 gene, which encodes the 5-HT6 receptor, is located on the short arm of human chromosome 1 at cytogenetic band 1p36.13, specifically spanning genomic coordinates 19,664,875 to 19,680,966 on the forward strand.^[13]^[14] This positioning places it in a region previously mapped through hybrid cell panel analysis and fluorescence in situ hybridization, confirming its proximity to other serotonin receptor loci.^[15] The gene structure encompasses approximately 16 kb and consists of three exons, with the coding sequence primarily within the final exon, reflecting a compact organization typical of G protein-coupled receptor genes. The HTR6 open reading frame translates to a 440-amino-acid polypeptide, forming a glycoprotein with a calculated molecular weight of 46.95 kDa, consistent with its seven-transmembrane domain architecture.^[16]^[17] A notable genetic variation is the single nucleotide polymorphism rs1805054 (C267T), a synonymous substitution in exon 1 that does not alter the amino acid sequence.^[18] This polymorphism has been investigated in relation to psychiatric traits, with some evidence for modulation of antidepressant treatment response in major depressive disorder, though associations with susceptibility to mood disorders and depression in Alzheimer's disease patients have not been consistently replicated.^[19]^[20]^[21] The 5-HT6 receptor exhibits strong evolutionary conservation across mammals, underscoring its fundamental role in serotonin signaling. Sequence identity reaches 89% between human and rat orthologs, with over 84% homology to the mouse counterpart, enabling reliable translation of findings from rodent models to human physiology despite subtle variations in the N-terminal and transmembrane regions that may influence ligand binding affinity or tissue distribution.^[22]^[23] These conserved features, including key residues in the binding pocket, have facilitated the development of transgenic rodent models for studying receptor function in neurological contexts.^[24] Recent genetic studies have also implicated HTR6 in non-psychiatric conditions. As of 2024, higher HTR6 expression in prostate tumors has been associated with neighborhood disadvantage and stress-related pathways, potentially contributing to aggressive prostate cancer risk.^[25] Additionally, elevated HTR6 expression was linked to decreased risk of lung adenocarcinoma in a 2025 study.^[26]

Expression Patterns and Distribution

The 5-HT6 receptor exhibits predominant expression within the central nervous system (CNS), with particularly high levels observed in several key brain regions. In rodents and humans, the receptor is densely localized in the striatum (including the caudate-putamen), nucleus accumbens, olfactory tubercle, hippocampus, and cerebral cortex, as well as the amygdala and choroid plexus.^[1]^[27] These regions are implicated in processes such as cognition, reward, and memory, though functional details are addressed elsewhere. Lower but detectable expression occurs in areas like the substantia nigra, thalamus, and spinal cord.^[28] In contrast, 5-HT6 receptor expression is low to negligible in peripheral tissues. Studies in rodents have reported faint or absent mRNA and protein levels in organs such as the liver, kidney, gastrointestinal tract, and heart, underscoring the receptor's CNS selectivity.^[1] This distribution pattern supports the receptor's primary role in neural functions rather than peripheral physiology. Developmentally, 5-HT6 receptor expression emerges early in rat brain ontogeny, with high mRNA levels detectable as soon as embryonic day 12 (E12), coinciding with serotonergic neuron differentiation. Expression slightly decreases by E17 but stabilizes and increases postnatally, reaching peak abundance in adulthood, particularly in striatal and cortical regions.^[29]^[1] Detection of 5-HT6 receptor expression relies on established molecular and histological techniques. In situ hybridization (ISH) is commonly used to map mRNA distribution at cellular resolution in brain sections, revealing neuronal and glial localization. For protein assessment, autoradiography with radiolabeled ligands like [125I]SB-258585 quantifies receptor density in tissue slices, while immunohistochemistry (IHC) visualizes subcellular localization, such as in primary cilia of neurons and astrocytes. Reverse transcription polymerase chain reaction (RT-PCR) further confirms mRNA abundance in dissected tissues.^[1]^[27]^[30]

Signaling Pathways

G-protein Coupling and Activation

The 5-HT6 receptor exclusively couples to the stimulatory heterotrimeric G-proteins Gs and G_olf, which upon activation stimulate adenylyl cyclase to elevate intracellular cyclic AMP (cAMP) levels. This coupling is a hallmark of the receptor's signaling, distinguishing it from other serotonin receptor subtypes that may engage Gi/o or Gq/11 pathways.^[7] Ligand binding to the orthosteric site induces key conformational changes in the receptor, including an outward displacement of transmembrane helix 6 (TM6) by approximately 7.9 Å, which opens the intracellular G-protein binding pocket. This rearrangement enables intracellular loop 2 (ICL2) to form a short α-helix that interacts directly with the C-terminal α5 helix of the Gs α-subunit, stabilizing the complex. The interaction promotes a disorder-to-order transition in the Gαs helical domain (H1 to disorder, H5 to order), facilitating GDP release and subsequent GTP binding, which dissociates the Gαs from the βγ subunits and initiates downstream signaling.^[2] The 5-HT6 receptor displays high constitutive activity, characterized by significant basal coupling to Gs even in the absence of agonist, reaching up to 50% of the maximal response elicited by full agonists. This basal activity is structurally underpinned by a hydrogen bond between Thr280^{6.47} in TM6 and Asn312^{7.45} in TM7, which biases the receptor toward the active conformation and reduces allosteric modulation by sodium ions. Mutations disrupting this bond, such as T280^{6.47}C, substantially attenuate constitutive signaling, confirming its mechanistic role.^[2] Species-specific variations affect coupling efficiency; the human 5-HT6 receptor exhibits higher potency and efficacy in Gs-mediated responses compared to rodent orthologs, potentially due to differences in the ligand-binding pocket and pharmacological profiles. For example, mouse 5-HT6 receptors show lower expression levels and distinct ligand affinities relative to human and rat counterparts, which may modulate overall coupling dynamics.^[31]^[32]

Downstream Effector Systems

The primary downstream effector system of the 5-HT6 receptor involves its canonical coupling to the stimulatory G protein (Gs), which activates adenylyl cyclase to increase intracellular cyclic adenosine monophosphate (cAMP) levels. This elevation in cAMP subsequently activates protein kinase A (PKA), which phosphorylates downstream targets including the cAMP response element-binding protein (CREB) at serine 133, promoting CREB dimerization and its binding to DNA response elements to regulate gene transcription associated with neuronal plasticity and survival.^[33]^[34] Beyond this cAMP-PKA-CREB axis, 5-HT6 receptor activation interacts with the mechanistic target of rapamycin complex 1 (mTORC1) pathway, where the receptor physically associates with mTOR and regulatory associated protein of mTOR (Raptor), leading to enhanced mTORC1 activity that supports protein synthesis and neuronal growth processes. Recent 2025 studies have demonstrated that selective 5-HT6 modulators can influence mTOR-dependent neuronal differentiation in neurodevelopmental models, highlighting this pathway's role in cognitive regulation.^[35]^[36]^[28] In certain cellular contexts, such as oligodendrocyte precursor cells, 5-HT6 receptor stimulation modulates phospholipase C (PLC) activity, contributing to intracellular calcium mobilization and cytokine secretion. Additionally, the receptor links to the extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase (MAPK) pathway, often via PKA-dependent mechanisms involving Fyn kinase, which promotes ERK1/2 phosphorylation and supports cell proliferation and differentiation.^[37]^[38]^[39] Feedback regulation of 5-HT6 receptor signaling occurs through β-arrestin-independent mechanisms, including PKA-mediated phosphorylation of the receptor itself or associated proteins like sorting nexin 14 (SNX14), which reduces Gs coupling efficiency and attenuates downstream cAMP accumulation over prolonged agonist exposure.^[40]^[41]

Physiological Functions

Role in the Central Nervous System

The 5-HT6 receptor contributes to cognitive processes in the central nervous system by enhancing memory consolidation and synaptic plasticity, particularly within the hippocampus. Activation of these receptors modulates glutamate release and NMDA receptor function, promoting long-term potentiation (LTP) and neuronal excitability essential for learning.^[42] This effect is mediated through Gs-protein coupling, which stimulates adenylyl cyclase to increase cyclic AMP (cAMP) levels and activate protein kinase A (PKA), thereby supporting neurite growth and synaptic strengthening.^[42] Studies demonstrate that 5-HT6 agonists, such as WAY-208466, improve emotional memory in rodent models by boosting hippocampal plasticity.^[42] In the striatum, 5-HT6 receptors regulate the release of dopamine and glutamate, influencing reward processing and motor control. These receptors are expressed on medium spiny neurons in both direct and indirect pathways, where their activation excites GABAergic interneurons and opposes dopamine's differential effects on pathway balance.^[43] Overexpression of 5-HT6 in indirect pathway neurons delays reward-motivated learning in the dorsomedial striatum while enhancing behavioral flexibility and reducing habit formation in the dorsolateral striatum.^[43] Such modulation integrates serotonergic inputs with dopaminergic signaling to fine-tune striatal circuits involved in motivation and movement.^[43] The 5-HT6 receptor plays a key role in neurodevelopmental processes, as evidenced by studies in knockout mice. 5-HT6 receptor-null mice display deficits in social recognition and increased anxiety-like behaviors, such as reduced exploration in open-field tests and elevated ambulatory activity.^[44]^[45] These animals also exhibit cognitive impairments, including prolonged escape latencies in the Morris water maze and reduced hippocampal LTP, alongside altered dendritic complexity in CA1 pyramidal neurons and dentate gyrus granule cells.^[44] Disruptions in Sonic Hedgehog signaling within primary cilia further highlight the receptor's involvement in early brain development.^[44] In Alzheimer's disease models, 5-HT6 receptor overexpression correlates with amyloid pathology and cognitive decline. In APP/PS1 transgenic mice, upregulated 5-HT6 expression in the hippocampus is associated with elongated primary cilia and impaired axonal morphology, exacerbating amyloid-beta accumulation and neuronal dysfunction. This overexpression contributes to deficits in spatial memory and fear conditioning, underscoring the receptor's pathological role in disease progression.^[46]

Implications in Peripheral Tissues

Although the 5-HT6 receptor is predominantly expressed in the central nervous system, low levels of its mRNA have been detected in peripheral immune tissues, including the thymus, spleen, and peripheral blood lymphocytes in rats.^[47] This expression pattern suggests a potential immunomodulatory role, particularly in immune cells where the receptor's canonical coupling to Gs proteins stimulates adenylate cyclase and elevates intracellular cAMP levels. Elevated cAMP is known to influence T-cell proliferation and activation in serotonergic signaling contexts, providing a basis for 5-HT6-mediated regulation of immune responses despite the receptor's sparse peripheral distribution.^[48]^[47] In the gastrointestinal tract, 5-HT6 receptors contribute to the regulation of intestinal motility through interactions with the enteric serotonin system. Studies in animal models, such as male BALB/c and C57Bl/6 mice, demonstrate that both constitutively active and agonist-stimulated 5-HT6 receptors promote defecation under physiological and stress-induced conditions. Inverse agonists like SB-399885 and neutral antagonists like CPPQ significantly attenuate gastrointestinal transit and reduce fecal output, indicating that 5-HT6 activation facilitates propulsive motility in the ileum, where receptor expression is detectable albeit at low levels. These findings highlight the receptor's involvement in enteric nervous system function, with implications for disorders like irritable bowel syndrome with diarrhea (IBS-D).^[49] Emerging evidence links 5-HT6 receptors to metabolic regulation, particularly in adipose tissue. In mesenchymal stromal cells (MSCs) derived from human adipose tissue, the receptor mediates serotonin-induced enhancements in α1A-adrenoceptor expression and Ca²⁺ signaling, thereby increasing cellular contractility and sensitivity to noradrenaline. This peripheral 5-HT/HTR6 axis contributes to obesity-associated dysfunctions, such as hypertension, by amplifying adrenergic responses in perivascular adipose regions. Rodent models of obesity further show that 5-HT6 antagonism reduces body weight, visceral adiposity, and insulin resistance, improving glycemic control; preliminary data from 2023-2024 studies reinforce HTR6 as a modulator of adipose remodeling and metabolic homeostasis, though human validation remains ongoing.^[50]^[51] Compared to its robust Gs-mediated cAMP signaling in the central nervous system, 5-HT6 receptor coupling appears weaker in peripheral tissues, potentially due to lower expression and subcellular localization differences. This variability underscores distinct physiological roles outside the brain, emphasizing the need for tissue-specific investigations.^[52]

Pharmacology and Ligands

Agonists

The endogenous ligand for the 5-HT6 receptor is serotonin (5-hydroxytryptamine, 5-HT), which binds with moderate affinity (Ki ≈ 65 nM at human receptors) and functions as a full agonist, producing maximal stimulation of adenylyl cyclase activity through Gs protein coupling.^[53] This activation leads to elevated intracellular cAMP levels, representing 100% efficacy in functional assays.^[48] Synthetic full agonists of the 5-HT6 receptor include compounds such as EMDT (2-ethyl-5-methoxy-N,N-dimethyltryptamine), which demonstrates high binding affinity (Ki ≈ 16 nM) and full efficacy comparable to serotonin in promoting cAMP accumulation, with selectivity over other serotonin receptor subtypes.^[54] Another example is lysergide (LSD), exhibiting even higher affinity (Ki ≈ 4 nM) while maintaining full agonism in downstream signaling pathways.^[48] These ligands have been instrumental in probing receptor activation without off-target effects at related 5-HT receptors. Partial agonists, such as E-6801, bind with high potency (Ki ≈ 3.5 nM) but display reduced intrinsic efficacy (approximately 60% relative to 5-HT in cAMP response assays), making them valuable tools for selectivity studies that isolate 5-HT6-mediated effects from full activation.^[55] These compounds typically show moderate selectivity profiles, with lower affinities at other 5-HT subtypes like 5-HT2A or 5-HT7. Structure-activity relationship analyses of 5-HT6 agonists highlight the critical role of a basic nitrogen atom, which facilitates ionic bonding with aspartate residues in the receptor's orthosteric site, alongside hydrophobic moieties that stabilize binding via aromatic or aliphatic interactions.^[56] Modifications to these elements, such as incorporating tryptamine scaffolds with alkyl substitutions, can optimize affinity and efficacy while preserving selectivity.

Antagonists and Inverse Agonists

Antagonists of the 5-HT6 receptor competitively bind to the orthosteric site, preventing agonist-induced activation and thereby inhibiting downstream signaling such as adenylyl cyclase stimulation.^[55] Inverse agonists, in contrast, not only block agonist effects but also suppress the receptor's constitutive activity, reducing basal signaling levels even in the absence of agonists.^[55] This distinction is particularly relevant for the 5-HT6 receptor, which exhibits measurable constitutive activity in certain expression systems.^[57] Prominent selective antagonists include SB-258585, a sulfonamide derivative with high affinity for the human 5-HT6 receptor (pKi = 8.5, equivalent to Ki ≈ 3 nM) and greater than 100-fold selectivity over 10 other 5-HT receptor subtypes and three additional receptors (dopamine D2, D3, and adrenergic α1B).^[58] Similarly, Ro 04-6790, a sulfonamide-based compound, displays potent antagonism at both rat (pKi = 7.4–7.6) and human (pKi = 7.3–7.9) 5-HT6 receptors, with over 100-fold selectivity relative to 23 other receptors, including dopamine and adrenergic subtypes.^[59] These compounds have been instrumental in pharmacological studies dissecting 5-HT6 receptor functions due to their clean profiles. SB-271046 serves as a well-characterized inverse agonist, exhibiting high affinity (pKi = 8.9 at human 5-HT6 receptors) and competitively antagonizing 5-HT-stimulated adenylyl cyclase activity while also inhibiting basal cAMP production in cells expressing the receptor.^[60] In functional assays, SB-271046 reduces constitutive cAMP accumulation, confirming its inverse agonistic properties alongside antagonist effects.^[55] Like other inverse agonists such as Ro 04-6790, it modulates receptor tone by counteracting ligand-independent signaling.^[55] Recent advancements have introduced neutral antagonists, which block agonist binding without altering constitutive activity, offering a tool to isolate orthosteric blockade effects. A 2024 study developed a series of 2-phenylpyrrole derivatives as selective neutral antagonists at 5-HT6 receptor-coupled Gs signaling, with lead compounds demonstrating potent binding (Ki in the low nanomolar range) and no reduction in basal cAMP levels, distinguishing them from inverse agonists.^[61] In 2025, novel antagonists such as PUC-10 and PUC-55 were reported as mTOR-dependent autophagy inducers.^[36] Achieving selectivity for 5-HT6 antagonists remains challenging due to homology with other G-protein-coupled receptors, leading to off-target interactions in some compounds at 5-HT2A receptors or dopamine subtypes, which can complicate interpretation of preclinical data.^[48] However, optimized ligands like SB-258585 and SB-271046 minimize such issues, showing negligible affinity (pKi < 6) at these sites.^[58]

Therapeutic Applications

Antagonists as Drug Targets

The 5-HT6 receptor has emerged as a promising therapeutic target for cognitive enhancement, particularly in Alzheimer's disease and schizophrenia, where antagonists have demonstrated potential to alleviate deficits in memory and executive function. Preclinical studies in rodent models of these disorders have consistently shown that 5-HT6 antagonists improve performance in learning and memory tasks, such as the Morris water maze and novel object recognition, by modulating striatal dopamine release to enhance synaptic plasticity and attention.^[62]^[63]^[64] The therapeutic rationale for 5-HT6 antagonism lies in its ability to block inhibitory signaling that suppresses key neurotransmitter systems involved in cognition. Specifically, 5-HT6 receptor blockade reduces tonic inhibition on cholinergic and glutamatergic pathways in the prefrontal cortex and hippocampus, leading to increased acetylcholine and glutamate efflux that supports synaptic strengthening and cognitive processing. This mechanism contrasts with direct agonists and has been linked to pro-cognitive effects without disrupting baseline neurotransmission.^[65]^[66] Clinical translation has faced challenges, as exemplified by idalopirdine, a selective 5-HT6 antagonist that advanced to Phase III trials as an adjunct to cholinesterase inhibitors in mild-to-moderate Alzheimer's disease but failed to meet primary endpoints for cognitive improvement in 2016, despite earlier Phase II signals of benefit. Other candidates like AVN-322, a highly selective 5-HT6 antagonist, completed Phase I trials demonstrating good tolerability and cognitive reversal in animal models of impairment, but no further clinical development has been reported as of 2025.^[67]^[68] More recently, masupirdine (SUVN-502), a potent and selective 5-HT6 receptor antagonist, is being evaluated in a Phase 3 clinical trial (NCT05397639) for the treatment of agitation associated with dementia of the Alzheimer's type, either as monotherapy or adjunct to standard care. As of November 2025, the trial is recruiting participants to assess efficacy, safety, and tolerability.^[4] 5-HT6 antagonists generally exhibit a favorable side effect profile, with clinical and preclinical data indicating good tolerability at therapeutic doses and no significant cardiovascular or gastrointestinal issues reported. Their mechanism, which avoids strong dopaminergic reward pathway activation, suggests low abuse potential, aligning with observations in early human studies where no dependency or withdrawal effects were noted.^[69]^[70]

Agonists and Modulators as Drug Targets

Activation of the 5-HT6 receptor by agonists increases intracellular cyclic adenosine monophosphate (cAMP) levels through Gs protein coupling, particularly in limbic brain regions like the striatum and hippocampus, which play key roles in emotional processing and reward pathways.^[33] This enhanced cAMP signaling underpins the rationale for 5-HT6 agonists as potential treatments for depression, where preclinical evidence shows antidepressant-like effects in rodent models such as the forced swim test and tail suspension test.^[71] Similarly, agonists like the partial agonist E-6837 promote hypophagia and sustained weight loss in diet-induced obese rats, indicating therapeutic promise for obesity by modulating feeding behavior and energy balance without significant cardiovascular side effects.^[51] Preclinical anxiolytic effects have also been observed with selective agonists such as WAY-208466, which reduce anxiety-like behaviors in elevated plus-maze and open-field tests, supporting their role in alleviating mood disorders.^[72] Positive allosteric modulators (PAMs) and partial agonists offer a strategy to fine-tune 5-HT6 receptor activity, enhancing endogenous serotonin signaling without the risks of full agonism, such as receptor desensitization.^[73] For instance, the partial agonist EMD386088 demonstrates antidepressant and anxiolytic effects following intrahippocampal administration in rats, improving performance in behavioral despair models while maintaining a balanced activation profile. This approach could mitigate overactivation concerns, allowing precise modulation in limbic circuits to support mood stabilization. Despite these preclinical advances, clinical development of 5-HT6 agonists remains limited, primarily due to the receptor's pronounced constitutive activity, which sustains basal signaling and may reduce the therapeutic window for agonists by promoting tolerance or off-target effects.^[11] No 5-HT6 agonists have progressed beyond early-phase trials as of 2025, with efforts hampered by challenges in achieving selectivity and managing potential neuropsychiatric side effects. Emerging research highlights links between 5-HT6 modulation and the mTOR pathway, where agonists or targeted modulators could influence neuronal autophagy and synaptic plasticity, offering novel avenues for neurodevelopmental disorders like autism spectrum disorder.^[36]

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Novel 5-HT 6 R modulators as mTOR-dependent neuronal ... - Nature
Mar 11, 2025 · Recent research has shown that the 5-HT6 receptor is linked to the mTOR pathway and can affect cognition in various neurodevelopmental models.
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The 5-HT and PLC Signaling Pathways Regulate the Secretion of IL ...
These studies suggest that 5-HT, acting on its receptors, is involved in cortical excitability that affects sleep regulation. PLC mediates cellular signaling ...
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[PDF] The 5-HT6 receptor interactome - HAL Um - Université de Montpellier
The mechanism whereby 5-HT6 receptor constitutive activity is abolished in the cilium remains to be fully elucidated. Treatment of cells harboring ciliary ...
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Activation of ERK1/2 through 5-HT 6 R is dependent on Fyn and ...
As a Gs protein-coupled receptor, activation of 5-HT 6 receptors has been demonstrated to enhance AC-cAMP-PKA-MAPK(ERK)-CREB signaling [22, 57] . In this ...
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SNX14 is a bifunctional negative regulator for neuronal 5-HT6 ...
We further found that protein kinase A (PKA)-mediated phosphorylation of SNX14 inhibited its binding to Gαs and diverted SNX14 from Gαs binding to 5-HT6R ...
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SNX14 is a bifunctional negative regulator for neuronal 5‐HT 6 ...
We further found that protein kinase A (PKA)‐mediated phosphorylation of SNX14 inhibited its binding to Gαs and diverted SNX14 from Gαs binding to 5‐HT6R ...
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https://hal.umontpellier.fr/hal-02364282v1/document
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https://www.researchgate.net/figure/Activation-of-ERK12-through-5-HT-6-R-is-dependent-on-Fyn-and-PKA-A-time-course-of_fig4_6611775
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https://journals.biologists.com/jcs/article/128/9/1848/55515/SNX14-is-a-bifunctional-negative-regulator-for
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mRNA Expression of Serotonin Receptors in Cells of the Immune ...
In the present study we used RT-PCR methods to examine the mRNA expression of 5-HT receptors in the cells of lymphoid tissues of the rat.
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5-HT 6 receptor - IUPHAR/BPS Guide to PHARMACOLOGY
5-HT6 | serotonin receptor 6 | ST-B17 | 5-hydroxytryptamine (serotonin) ... Thymus, peripheral blood lymphocytes, spleen, mitogen-activated spleen cells.
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Serotonin type 6 receptor inverse agonists and neutral antagonists ...
The present findings confirm the contribution of both constitutively active and agonist-stimulated serotonin 5-HT6 receptors to intestinal motility and diarrhea ...
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Peripheral 5-HT/HTR6 axis is responsible for obesity-associated ...
Discovered effect of HTR6 on MSCs contractility points to it as a potential therapeutic target for the prevention and treatment of obesity-associated ...
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Chronic 5-HT6 receptor modulation by E-6837 induces hypophagia ...
... 5-HT6 receptor ligand ... These data are probably of biological significance indicating an improvement in insulin sensitivity, and hence, glycemic control.
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Impact of 5-HT6 Receptor Subcellular Localization on Its Signaling ...
The 5-HT6R is the only serotonin receptor displaying a ciliary location, suggesting a special role for this receptor in neurodevelopment and brain function. The ...
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Serotonin Receptor Subtypes and Ligands - ACNP
Seven distinct families of 5-HT receptors have been identified (5-HT1–5-HT7), and subpopulations have been described for several of these.
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EMDT oxalate | CAS 263744-72-5 - Tocris Bioscience
$$45 deliverySelective 5-HT6 agonist (with a Ki value of 16 nM at human 5-HT6 receptors). Exhibits potency comparable to serotonin for the activation of adenylate cyclase.
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Efficacy of selective 5-HT6 receptor ligands determined by ...
It may have a primary role in memory processes and learning, and 5-HT6 receptor ligands may be of benefit to improve cognitive function as suggested by a list ...
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(PDF) Investigation of the Structure Requirement for 5-HT6 Binding ...
Aug 8, 2011 · ... 22 The commonly recognized pharmacophore of the 5-HT 6 R antagonists consists of four key fragments: basic nitrogen atom (PI), hydrophobic ...
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A Novel in vivo Anti-amnesic Agent, Specially Designed to Express ...
Jun 18, 2019 · Of interest, compound 10, idalopirdine and SB271046 were able to reverse the constitutive activity of the 5-HT6 receptor, thus demonstrating an ...
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Characterization of [125I]-SB-258585 binding to human recombinant ...
5-Hydroxytryptamine (5-HT) exerts a wide variety of physiological and behavioural effects through actions on multiple receptor subtypes. These receptors have ...Cloned Receptors · Radioligand Binding · Results<|control11|><|separator|>
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Characterization of Ro 04-6790 and Ro 63-0563: potent and ...
Feb 3, 2009 · Both compounds were found to be over 100 fold selective for the 5-HT6 receptor compared to 23 (Ro 04-6790) and 69 (Ro 63-0563) other receptor ...
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Characterization of SB-271046: A potent, selective and orally active ...
The development of the selective 5-HT6 receptor antagonist, Ro 04-6790, was a significant improvement over antisense treatment, however, whilst this compound ...
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5-HT6 receptor neutral antagonists protect astrocytes: A lesson from ...
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5-HT6 Receptor Antagonists as Novel Cognitive Enhancing Agents ...
A more recent study has confirmed that intrahippocampal administration of a 5-HT6 antagonist (SB-258585) produces an anxiolytic and antidepressant profile in ...Missing: Ki | Show results with:Ki
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5-HT6 Receptor Antagonists: Potential Efficacy for the Treatment of ...
5-hydroxytryptamine6 receptor (5-HT6R) antagonists have shown efficacy in animal models for cognitive impairment in multiple cognitive domains relevant for ...
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In vivo effects of the 5-HT6 antagonist SB-271046 on striatal ... - NIH
In vivo effects of the 5-HT6 antagonist SB-271046 on striatal and frontal cortex extracellular concentrations of noradrenaline, dopamine, 5-HT, glutamate and ...
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5-HT6 receptor antagonists as novel cognitive enhancing agents for ...
It is increasingly clear that blockade of 5-HT 6 receptors leads to an improvement of cognitive performance in a wide variety of learning and memory paradigms.
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Symptomatic AD treatment fails in first phase III - Nature
Nov 3, 2016 · Lundbeck's and Otsuka Pharmaceutical's idalopirdine failed in a first phase III trial in patients with mild to moderate Alzheimer disease ...
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5HT6 Antagonists in the Treatment of Alzheimer's Dementia
Early trials of the 5HT6 antagonists showed improvements in cognition and activities of daily living when used as adjuncts to current therapies for Alzheimer's ...
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Safety, Tolerability and Pharmacokinetics of the Serotonin 5-HT6 ...
Aug 19, 2021 · HEC30654 is a selective 5-HT6 receptor antagonist that was safe and well-tolerated in preclinical models of Alzheimer's disease.
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A novel highly selective 5-HT6 receptor antagonist attenuates ...
These results indeed suggest that the 5-HT6 receptor may be a viable target for the control of drug abuse.Missing: low | Show results with:low
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Antidepressant and anxiolytic effects of selective 5-HT6 receptor ...
In the current study, we tested the hypothesis that activation of the 5-HT6 receptor would produce antidepressant-like and anxiolytic-like effects in rats.
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Neuropharmacological Profile of Novel and Selective 5-HT6 ...
Jul 11, 2007 · The serotonin (5-HT, 5-hydroxytryptamine) receptor family currently consists of 14 members separated into seven classes (5-HT1–7). One of the ...
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Allosteric Modulators of Serotonin Receptors: A Medicinal Chemistry ...
May 28, 2024 · The present review describes the identification of allosteric ligands of serotonin receptors, which are largely natural compounds (oleamide, cannabidiol, THC, ...Missing: 5214757 | Show results with:5214757