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ABCC11

ABCC11 is a human gene located on chromosome 16q12.1 that encodes a 1,382-amino-acid protein belonging to the ATP-binding cassette (ABC) transporter superfamily, specifically multidrug resistance-associated protein 8 (MRP8).^[1] This protein features 12 transmembrane helices forming two membrane-spanning domains and two nucleotide-binding domains that hydrolyze ATP to drive the efflux of substrates across cellular membranes.^[1] As an apical efflux pump predominantly expressed in apocrine glands, ABCC11 transports lipophilic anions, including steroid sulfates, cyclic nucleotides, and glutathione conjugates, contributing to the secretion of metabolites in sweat, cerumen, and colostrum.^[1] A key functional variant in ABCC11 is the single nucleotide polymorphism rs17822931 (c.538G>A), which causes a glycine-to-arginine substitution at amino acid position 180 (p.Gly180Arg), resulting in a loss-of-function allele.^[1] The ancestral G allele (wet earwax phenotype) supports active transport and is associated with sticky, wet cerumen, while the derived A allele (dry earwax phenotype) impairs protein function, leading to flaky, dry cerumen and reduced apocrine secretions.^[1] This SNP exhibits strong population stratification, with the A allele frequency reaching nearly 100% in Northeast Asian populations (e.g., Koreans, Japanese) and being rare (<3%) in Europeans and Africans, reflecting historical natural selection possibly linked to environmental adaptations like cold climates or pathogen resistance.^[2] The physiological significance of ABCC11 extends to axillary osmidrosis, where the functional G allele enables the transport of odorless precursor molecules (e.g., S-glutathionyl-3-methyl-3-sulfanylhexan-1-ol conjugates) into sweat, which axillary bacteria such as Staphylococcus hominis metabolize via enzymes like PatB into volatile 3-methyl-3-sulfanylhexan-1-ol (3M3SH), the primary malodorous compound.^[3] Individuals homozygous for the A allele (AA genotype) exhibit minimal body odor due to deficient precursor secretion and altered skin microbiome composition favoring less odor-producing bacteria like Corynebacterium.^[3] Beyond sensory traits, ABCC11 variants influence clinical outcomes, serving as a potential biomarker for increased breast cancer risk in Japanese women (odds ratio 1.63 for G allele carriers) and modulating responses to nucleoside analog chemotherapies, such as 5-fluorouracil resistance in colorectal cancer patients.^[1]

Gene and Protein

Genomic Organization

The ABCC11 gene is located on the long arm of chromosome 16 at position 16q12.1, spanning approximately 82 kb from base pair 48,165,773 to 48,247,568 (GRCh38.p14 assembly).^[4] It was identified in 2001 through screening of expressed sequence tag (EST) databases and gene prediction programs as a novel member of the ATP-binding cassette (ABC) transporter superfamily, alongside the closely related ABCC12 gene, which lies approximately 20 kb upstream in a tail-to-head orientation.^[5] The gene consists of 31 exons in its canonical form, with the full-length transcript encoding a 1,382-amino-acid protein.^[6] The exon-intron architecture of ABCC11 features variable exon sizes ranging from 46 bp (exon 2) to 1,594 bp (exon 31), interrupted by introns that collectively account for the majority of the gene's length, enabling precise regulation of splicing.^[6] The promoter region, located upstream of the transcription start site, contains binding sites for transcription factors such as ATF-2 and c-Jun, which contribute to tissue-specific expression, particularly in apocrine glands and breast tissue.^[7] Known regulatory elements include enhancers within the intronic and flanking regions, as annotated in the Ensembl regulatory build, which influence expression levels in response to hormonal and developmental cues.^[8] The primary transcription start site is at nucleotide 48,247,568 (GRCh38), initiating the full-length mRNA isoform NM_032583.3, which measures 6,094 bp and undergoes polyadenylation at multiple sites, including position 6,094.^[6] Alternative splicing generates at least nine transcripts, including isoform b (NM_145186), which lacks exon 28 and represents about 25% of total ABCC11 mRNAs in certain tissues, potentially modulating transporter function without altering the core ABC domains.^[4]^[7] Other variants, such as NM_001370496 (isoform c), introduce shorter N-terminal sequences but retain the transmembrane and nucleotide-binding domains essential for activity.^[4]

Protein Structure and Transport Mechanism

ABCC11, also known as multidrug resistance protein 8 (MRP8), belongs to the ATP-binding cassette (ABC) subfamily C, which comprises MRP-like transporters characterized by their ability to efflux a diverse array of substrates across cellular membranes.^[5] As a full ABC transporter, ABCC11 consists of two transmembrane domains (TMD1 and TMD2), each comprising six alpha-helical segments that form a total of 12 transmembrane helices, and two cytoplasmic nucleotide-binding domains (NBD1 and NBD2) responsible for ATP binding and hydrolysis.^[9] These structural elements are highly conserved within the ABCC subfamily, enabling the protein to span the lipid bilayer and couple energy from nucleotide hydrolysis to active transport.^[10] The transport mechanism of ABCC11 relies on ATP hydrolysis at the NBDs to drive the efflux of lipophilic anions from the cytoplasm to the extracellular space. Upon ATP binding, the NBDs undergo dimerization, inducing conformational changes in the TMDs that facilitate substrate translocation across the membrane in an alternating access model.^[1] ABCC11 specifically transports amphipathic anions, including glutathione conjugates such as leukotriene C4 and dinitrophenyl S-glutathione, steroid sulfates like dehydroepiandrosterone 3-sulfate, and other compounds such as cyclic nucleotides (cAMP and cGMP) and estradiol 17-β-D-glucuronide.^[11] This ATP-dependent process ensures the extrusion of these physiological and xenobiotic substrates, contributing to cellular homeostasis.^[12] Homology modeling of ABCC11's three-dimensional structure, based on closely related ABCC family members like ABCC1 (MRP1), predicts a dimeric architecture where the TMDs form a central substrate-binding cavity flanked by the NBDs at the cytoplasmic face.^[13] The dimerization interfaces primarily involve ATP-sandwiched contacts between the NBDs, with key residues in the Walker A and B motifs and signature sequences facilitating hydrolysis and reset of the transport cycle.^[14] These models highlight two conformational states: an inward-facing open state for substrate entry and an outward-facing closed state post-ATP binding, analogous to structures resolved for ABCC1 via cryo-electron microscopy.^[15] ABCC11 is synthesized and initially localized to the endoplasmic reticulum (ER), where N-linked glycosylation at specific asparagine residues (e.g., Asn838 and Asn844) stabilizes the protein and promotes its maturation.^[1] Proper folding and glycosylation enable trafficking through the secretory pathway to the apical plasma membrane in polarized epithelial and secretory cells, such as those in apocrine glands, ensuring directional efflux.^[16] Disruptions in this ER-to-apical trafficking can lead to intracellular retention, underscoring the importance of post-translational modifications for functional membrane insertion.^[17]

Physiological Roles

Cerumen Secretion and Earwax Type

The ABCC11 gene encodes an ATP-binding cassette transporter protein that is highly expressed in the ceruminous glands lining the external auditory canal, where it facilitates the secretion of cerumen, or earwax. These modified apocrine glands produce earwax to protect the ear canal by trapping dust and bacteria, and ABCC11's transport activity is essential for the biochemical composition of this secretion. In individuals with functional ABCC11, the protein actively exports lipid precursors into the glandular lumen, enabling the formation of wet earwax, which appears sticky, honey-colored, and contains higher levels of lipids compared to its dry counterpart.^[18]^[11] The biochemical pathway involves ABCC11 transporting lipid precursors and lipophilic anions from the cytoplasm to the extracellular space within ceruminous gland cells. These transported lipids contribute to the viscous consistency and protective properties of wet cerumen by integrating with sebum and desquamated skin cells. Dysfunction in this transport mechanism alters the lipid profile of secretions, shifting the phenotype toward dry earwax, which lacks these components and instead consists primarily of drier, keratin-based flakes. Recent studies indicate that ABCC11 genotypes also influence the composition of the ear canal microbiota and its metabolic pathways.^[11]^[19] A key genetic variant underlying this phenotypic difference is the single nucleotide polymorphism (SNP) 538G>A (rs17822931) in exon 4 of ABCC11, resulting in a glycine-to-arginine amino acid substitution at position 180 (Gly180Arg). This non-synonymous change is the primary molecular determinant of earwax type, with the G allele (wild-type) associated with wet earwax in homozygous G/G and heterozygous G/A individuals, while the A allele produces dry earwax only in homozygous A/A genotypes. The variant was first identified and linked to earwax phenotypes in a 2006 Japanese genome-wide association study.^[18] The Gly180Arg substitution abolishes a critical N-linked glycosylation site on ABCC11, leading to protein misfolding within the endoplasmic reticulum (ER). The misfolded variant is retained in the ER, where it undergoes ubiquitination and subsequent proteasomal degradation, preventing maturation and apical membrane localization in ceruminous gland cells. This loss of functional ABCC11 protein in A/A homozygotes eliminates lipid transport, thereby producing the recessive dry earwax trait and highlighting ABCC11's indispensable role in cerumen gland physiology.^[20]

Axillary Sweat and Body Odor Regulation

The ABCC11 protein, expressed in the apical membrane of secretory cells in axillary apocrine glands, functions as an ATP-binding cassette transporter that exports odorless precursor molecules into the sweat secretions. These precursors include cysteine-glutathione conjugates such as Cys-Gly-(E)-3-methyl-3-sulfanylhexan-1-ol (Cys-Gly-3M3SH), as well as glutamine conjugates like 3-methyl-2-hexenoic acid-glutamine (3M2H-Gln) and (E)-3-methyl-2-hexenoic acid-glutamine (HMHA-Gln), and steroid sulfates such as dehydroepiandrosterone sulfate (DHEAS).^[21]^[22] Once secreted, these non-volatile compounds are metabolized by resident skin bacteria, including Corynebacterium and Staphylococcus species, into volatile odorants responsible for axillary body odor, including 3-methyl-3-sulfanylhexan-1-ol (3M3SH) and short-chain fatty acids.^[21]^[1] The common single nucleotide polymorphism (SNP) 538G>A (rs17822931) in the ABCC11 gene disrupts this transport function, particularly in A/A homozygotes, leading to a non-functional protein that undergoes proteasomal degradation and fails to localize properly in apocrine gland cells. This results in reduced export of odor precursors and consequently minimal axillary osmidrosis, or body odor, in affected individuals.^[21] The variant's effect is most pronounced in A/A genotypes, where it abolishes the secretion of key thioalcohol precursors like Cys-Gly-3M3SH, preventing their bacterial conversion to odorous compounds.^[22] Quantitative analyses of axillary secretions reveal stark differences across genotypes: in A/A individuals, levels of Cys-Gly-3M3SH are undetectable, while 3M2H-Gln and HMHA-Gln are below detection limits or significantly lower compared to G/G and G/A carriers, where median concentrations reach 0.16–0.17 µmol for 3M2H-Gln and 0.85–1.18 µmol for HMHA-Gln. Similarly, steroidal odorants like 5α-androst-16-en-3-one show median levels of approximately 302 ng/ml in A/A versus 1371 ng/ml in G/G, representing an over 75% reduction.^[21]^[22] This transport activity aligns with the post-pubertal maturation of apocrine glands, as ABCC11 expression and function are upregulated following puberty under hormonal influences like estrogen, coinciding with the onset of noticeable axillary odor in individuals with functional alleles.^[1]

Other Tissue Functions

ABCC11 is expressed in normal breast tissue and plays a role in the transport of lipid metabolites during lactation, particularly as an efflux pump facilitating colostrum secretion.^[23] This function aligns with the broader capacity of ABCC transporters to handle lipophilic substrates, though specific lipid metabolites transported by ABCC11 in mammary glands remain under investigation.^[23] In the colon, ABCC11 contributes to the efflux of xenobiotics and endogenous toxins, supporting the intestinal barrier's protective mechanisms against harmful compounds.^[23] Its expression, detected via quantitative RT-PCR and immunohistochemistry, is notably lower in colon cancer tissues compared to normal mucosa, potentially influencing drug sensitivity such as to 5-fluorouracil.^[23] ABCC11 mRNA and protein have been identified in the placenta and prostate through RT-PCR and immunohistochemical analyses, suggesting a hypothetical role in steroid sulfate transport, including dehydroepiandrosterone 3-sulfate, as an ATP-dependent apical efflux pump. Expression levels are low in placental tissue but present in prostate, consistent with its involvement in handling steroid conjugates across membranes.^[23] Beyond these tissues, ABCC11 provides minor contributions to multidrug resistance in cancer cells, particularly in breast and gastrointestinal tumors, where elevated expression correlates with resistance to agents like eribulin and 5-fluorouracil, though it is less prominent than transporters such as ABCB1 or ABCC1.^[24]^[25]^[23] High ABCC11 levels in aggressive breast cancer subtypes are associated with poorer disease-free survival, highlighting its potential but limited role in chemotherapeutic efflux.^[24]

Genetic Variation

Key Polymorphisms and Mutations

The most prominent polymorphism in the ABCC11 gene is the single nucleotide variant rs17822931 (c.538G>A; p.Gly180Arg), a nonsynonymous change in exon 4 that results in an amino acid substitution from glycine to arginine at position 180 of the protein.^[18] The G allele, associated with wet earwax and higher odor production, is the ancestral form, while the derived A allele, linked to dry earwax and reduced odor, likely originated in Northeast Asia and spread through migration.^[18] In global populations, the minor allele frequency (MAF) of the A allele is approximately 0.28 as of gnomAD v4 (2024).^[26] Other notable variants include a 27-base pair in-frame deletion (Δ27) in exon 29, independent of rs17822931, observed primarily in East Asian populations and associated with the dry earwax phenotype in compound heterozygotes.^[18] Rare missense mutations, such as p.Arg1297Cys (rs141203646), have been reported in ClinVar and classified as benign for variations in apocrine gland secretion, though they occur at low frequencies (MAF <0.001 in gnomAD). Additional rare missense variants like p.Arg1325Trp and p.Phe1372Leu are cataloged in ClinVar with uncertain significance, often lacking strong evidence for pathogenicity. Haplotype analysis reveals a common East Asian haplotype carrying the derived A allele at rs17822931, often in linkage disequilibrium with neutral variants such as rs6500380 and ss49784070, forming a conserved block that distinguishes dry earwax carriers across populations.^[18] This haplotype structure contributes to the high prevalence of the A allele in Korean (0.96) and Japanese (0.80) cohorts, as reported in early genotyping studies.^[18] In more recent data from gnomAD v4 (2024), the A allele frequency in East Asians is approximately 0.90.^[26] ClinVar entries for ABCC11 variants, including copy number gains or losses, highlight potential pathogenic impacts in rare cases, but most polymorphisms remain benign or of uncertain significance for sensory traits.

Molecular Consequences of Variants

The primary genetic variant in ABCC11 associated with functional alterations is the nonsynonymous single nucleotide polymorphism (SNP) rs17822931 (538G>A), which results in a glycine-to-arginine substitution at amino acid position 180 (Gly180Arg).^[28] This change disrupts N-linked glycosylation at nearby asparagine residues (Asn183 and Asn189), causing misfolding particularly in the nucleotide-binding domain 1 (NBD1) of the protein.^[28] The misfolded protein is recognized by the endoplasmic reticulum (ER) quality control machinery, leading to ubiquitination and subsequent proteasomal degradation via ER-associated degradation (ERAD) pathways.^[28]^[1] In homozygous A/A individuals, this degradation mechanism results in a profound reduction in ABCC11 protein levels, often exceeding 90% compared to wild-type G/G carriers, with the variant protein frequently undetectable in apocrine glands.^[29] Heterozygotes (G/A) exhibit intermediate protein expression, though still substantially diminished relative to wild-type.^[29] mRNA levels remain comparable between wild-type and variant alleles, indicating that the reduction occurs at the post-transcriptional level, primarily through impaired protein stability and trafficking rather than altered mRNA stability or translation efficiency.^[29] Functional consequences include a significant loss of ATP-dependent transport activity. In vitro assays using substrates such as cyclic nucleotides (e.g., cGMP) and steroid sulfates (e.g., dehydroepiandrosterone sulfate) demonstrate 50-70% reduced efflux in cells expressing the A allele, with near-complete abolition in A/A homozygotes due to the absence of mature protein at the plasma membrane.^[1] Experimental evidence from cellular models supports these mechanisms. Since the seminal 2009 study, HEK293 cells transiently expressing the variant have shown ER retention and defective trafficking to the plasma membrane, confirming the degradation pathway.^[28] Complementary yeast (Saccharomyces cerevisiae) models, adapted for ABC transporter analysis, reveal variant-specific reductions in protein expression to approximately 20% of wild-type levels, underscoring conserved misfolding and instability across systems.^[1] These findings highlight the variant's dominant-negative impact on ABCC11 maturation and function.^[28]

Population Genetics

Global Allele Frequency Distribution

The A allele of the rs17822931 polymorphism in the ABCC11 gene, which is associated with the dry earwax phenotype, exhibits a striking global distribution characterized by high prevalence in East Asian populations and progressively lower frequencies in other groups. In East Asians, the allele frequency ranges from 80% to 95%, with reported values of 93% in Japanese (HapMap JPT) and Han Chinese (HapMap CHB) samples, and approximately 90% in Koreans.^[30] This high frequency contributes to near-universal dry earwax homozygosity (AA genotype) in these groups. In contrast, the A allele is virtually absent in African populations, with frequencies approaching 0%.^[31] Among Europeans, the frequency is substantially lower, typically 10-20%.^[32] Data from the 1000 Genomes Project indicate a global minor allele frequency (MAF) for the A allele of 0.301, reflecting its uneven distribution across continents, with the highest rates of AA homozygosity observed in Northeast Asian subpopulations.^[33] Frequencies in South Asians are approximately 48%, while in admixed populations such as those of Hispanic ancestry, they fall between 10% and 20%, influenced by varying degrees of European, Indigenous American, and African admixture; indigenous Native American populations exhibit higher frequencies, typically 30-90% depending on the group.^[34]^[35] The allele frequency displays a clinal gradient, decreasing from east to west across Asia, with intermediate values of 20-30% in Central Asian groups such as Uzbeks and Kazakhs, consistent with historical migration patterns from East Asia.^[31] Recent genomic surveys from 2023-2025, including large-scale exome sequencing in diverse cohorts, confirm the stability of these patterns in admixed populations, with Hispanic groups maintaining A allele frequencies around 10-20% despite ongoing gene flow.^[36]

Evolutionary and Anthropological Insights

The derived A allele at the ABCC11 rs17822931 locus (c.538G>A), associated with dry earwax and reduced axillary odor, originated in Northeast Asia as a recent founder mutation. Genetic analyses indicate that this allele arose approximately 2,000 generations ago (roughly 50,000 years, with a 95% credible interval of 1,023–3,901 generations), based on coalescent simulations using microsatellite data from global populations.^[2] More recent ancient DNA studies, including analysis of a ~44,000-year-old individual from the Tianyuan site in China, confirm the early presence of the derived allele in East Asia.^[37] Haplotype sharing across diverse groups, including Southern Chinese, Japanese, Native Americans, and Europeans with dry earwax, supports a single origin rather than independent mutations. Evidence points to positive selection driving the allele's rapid rise in frequency, particularly in East Asian populations. Genome-wide scans and linkage disequilibrium analyses reveal extended haplotype homozygosity and reduced genetic diversity around the locus in Japanese and Han Chinese samples compared to Yoruba Africans, consistent with a selective sweep.^[2] The selection coefficient is estimated at ~0.01 under a recessive model, with the signal strongest in Northeast Asian groups where the allele frequency approaches fixation (up to 95–100%).^[2] Although specific Tajima's D and iHS metrics are not detailed in primary studies on this locus, the overall patterns of low heterozygosity and long-range LD align with signatures of recent positive selection observed in similar adaptive traits. Hypothesized selective advantages include adaptations to cold climates, where reduced apocrine sweat and body odor may have minimized heat loss or bacterial growth in harsh environments. The allele's frequency correlates positively with absolute latitude across Asian, Native American, and European populations (P < 0.005), supporting a link to glacial-period conditions in Northeast Asia.^[2] Alternatively, in smaller isolated populations, neutral drift could contribute to elevated frequencies, though selection appears dominant in core East Asian demographics. Hygiene benefits, such as lower odor facilitating social interactions in dense groups, have also been proposed but remain speculative. Anthropologically, the A allele's distribution reflects historical human migrations from Northeast Asia. Frequencies decline in a north-south and east-west gradient, from near-fixation in Koreans and Northern Han Chinese (~80–100%) to lower levels in Southern Asians (~30–50%) and indigenous Siberians (~70–90%), mirroring post-Last Glacial Maximum expansions and admixture events. Higher prevalence in indigenous Siberian groups, such as Evenks and Yakuts, underscores ties to ancient Northeast Eurasian dispersals into Central Asia and the Americas. Recent genomic studies confirm minimal contribution from archaic hominins to ABCC11 variant diversity. The derived A allele is absent in sequenced Neanderthal and Denisovan genomes, which carry the ancestral G allele, indicating the mutation arose after modern human divergence from archaic lineages and spread via endogenous selection rather than introgression. 2024 analyses of East Asian ancient DNA further show no archaic-derived haplotypes at this locus, reinforcing its modern human-specific evolutionary history.^[37]

Clinical Significance

Associations with Sensory Traits

The rs17822931 single-nucleotide polymorphism (SNP) in the ABCC11 gene exhibits complete phenotypic penetrance for earwax type, with the homozygous AA genotype resulting in dry, flaky cerumen in 100% of individuals, while the GG and GA genotypes produce wet, sticky cerumen. This determination was established through genotyping and phenotyping of diverse populations, confirming the SNP as the sole genetic basis for the trait.^[18] The same variant governs axillary body odor intensity, where AA homozygotes secrete minimal odor precursors (such as cysteine-glycine conjugates of 3-methyl-3-sulfanylhexan-1-ol) in apocrine sweat, leading to reduced or undetectable odor; in East Asian populations with high A allele prevalence (80-95%), 80-90% of AA individuals exhibit no noticeable body odor.^[3]^[38] Sensory evaluations highlight genotype-specific differences in odor perception and hygiene behaviors. Individuals with the AA genotype demonstrate lower olfactory thresholds for detecting axillary odorants in controlled testing, correlating with reduced self-reported body odor intensity. Deodorant usage patterns also vary markedly, with AA homozygotes overrepresented among never or infrequent users; however, 77.8% of genotypically non-odorous (AA) white Europeans still used deodorant at least once a week.^[39] This behavioral distinction holds across ethnic groups and underscores the trait's influence on personal hygiene practices. Non-invasive genotyping via earwax swabs serves as a reliable proxy for A allele detection, enabling DNA extraction and SNP analysis without blood sampling. This method, validated in 2025 for educational applications, achieves high accuracy in correlating swab-based phenotypes with genotypes, facilitating accessible teaching tools for genetic variation.^[40] Twin and family studies affirm the heritability of earwax type and associated body odor traits at over 95%, reflecting the monogenic, mendelian inheritance pattern of ABCC11 variants with near-complete genetic control.^[32]

Links to Cancer and Other Diseases

The ABCC11 gene has been investigated for its potential role in breast cancer susceptibility, particularly through the common rs17822931 (538G>A) polymorphism, where the A allele results in a non-functional protein. In Asian cohorts, the homozygous A/A genotype has been linked to a reduced risk of breast cancer, with one Japanese case-control study reporting an odds ratio of 1.63 (95% CI: 1.05-2.52, p=0.026) for G allele carriers (wet earwax type) compared to A/A individuals, suggesting approximately 39% lower risk for the A/A genotype. This protective effect may arise from impaired lipid and xenobiotic transport in mammary gland apocrine cells, potentially limiting estrogen-related carcinogenesis, though the exact mechanism remains under study. However, conflicting results exist; a larger Japanese study found the G allele associated with decreased risk (OR=0.77 per G allele, 95% CI: 0.62-0.95, p=0.013), particularly for estrogen receptor-positive tumors in women with high estrogen exposure. In European populations, where the A allele is rare (frequency <3%), no significant association with breast cancer risk has been observed for the rs17822931 variant. These discrepancies highlight population-specific genetic interactions, with meta-analyses from 2010-2023 indicating inconsistent effects outside Asian groups. Regarding colorectal cancer, ABCC11 overexpression has been detected in tumor tissues, correlating with aggressive disease and poor prognosis. High ABCC11 expression in colorectal adenocarcinoma is associated with reduced disease-free survival and may contribute to chemoresistance by effluxing anticancer drugs such as 5-fluorouracil (5-FU). In vitro studies demonstrate that ABCC11 directly confers resistance to 5-FU, with overexpression lowering intracellular drug accumulation and enhancing cell survival. Though large-scale GWAS have not consistently replicated genetic effects of rs17822931 in colorectal cancer. Beyond cancers, ABCC11 variants show potential links to other conditions. Preliminary evidence suggests an association with axillary hidradenitis suppurativa, a chronic inflammatory disorder of apocrine glands, where patients exhibit a higher prevalence of the wet earwax phenotype (G allele) compared to general populations, possibly due to altered apocrine secretion.^[41] However, no causal role has been established. For hyperbilirubinemia, while ABCC11 shares functional similarities with other ABC transporters involved in bilirubin handling (e.g., ABCC2), direct associations remain speculative and unsupported by clinical data. Recent studies up to 2025, including pharmacogenomic analyses, indicate no strong link between ABCC11 polymorphisms and metabolic syndromes such as diabetes or obesity, emphasizing its more restricted role in transport-related pathologies.

Interactions with Microbiota

The ABCC11 gene encodes an ATP-binding cassette transporter that secretes odor precursors, such as cysteine-glycine-(S)-3-methyl-3-sulfanylhexanoyl conjugates, into apocrine sweat, influencing the axillary microbiota composition. Individuals homozygous for the loss-of-function allele (538A/A, dry earwax type) exhibit reduced ABCC11 transporter activity, leading to lower secretion of these precursors and a shift in microbial balance toward higher relative abundance of Staphylococcus species (up to 65%) and reduced Corynebacterium (as low as 1%), compared to the functional 538G/G genotype, which favors Corynebacterium dominance (up to 57%) and Staphylococcus at lower levels (around 29%). This alteration diminishes the prevalence of odor-producing bacteria like Corynebacterium tuberculostearicum, as the limited substrate availability selects against microbes dependent on these conjugates for metabolism.^[3] In the ear canal, ABCC11 variants similarly condition the microbial niche, with the dry earwax phenotype (538A/A) associated with distinct profiles characterized by higher Proteobacteria abundance, including enrichment in Methylocella species, while the wet earwax type (538G/A or G/G) is dominated by Staphylococcus auricularis and Corynebacterium species. A 2025 metagenomic study of healthy adults confirmed these differences, revealing that the non-functional variant correlates with altered metabolic potentials, such as enriched pathways for allantoin degradation and nitrifier denitrification, potentially due to reduced lipid and conjugate secretion affecting bacterial substrate access.^[19] These shifts highlight ABCC11's role in heritable microbiome structuring, independent of direct host pathology. ABCC11 variants impact microbial metabolic pathways by modulating substrate availability for bacterial enzymes, notably cysteine-S-conjugate β-lyase (PatB) in Staphylococcus hominis, which cleaves secreted conjugates to produce malodorous 3-methyl-3-sulfanylhexan-1-ol (3M3SH). Functional ABCC11 (C/C or C/T haplotypes) supports higher PatB expression and 3M3SH yields in axillary microbiomes, whereas the non-functional T/T haplotype reduces enzyme abundance and odor output, demonstrating a symbiotic host-microbe interaction. Recent 2025 research on genome-microbiome associations further elucidates this heritable niche conditioning, showing ABCC11 haplotypes predict microbial enzyme profiles across generations in familial pedigrees, underscoring evolutionary adaptations in human-microbe co-evolution.^[3]^[19]

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ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes physiological compounds and xenobiotics from cells.<|control11|><|separator|>
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[24]
ABCB1 and ABCC11 confer resistance to eribulin in breast cancer ...
Oct 25, 2016 · Both ABCB1 and ABCC11 are involved in the development of eribulin resistance in breast cancer cells in vitro regardless of the breast cancer subtype.Abcb1 And Abcc11 Confer... · Results · Inhibition Of Abcb1 Or...
[25]
ABCC11[gene] - ClinVar - NCBI
### Summary of ABCC11 Gene Polymorphisms and Mutations (ClinVar)
[26]
https://gnomad.broadinstitute.org/variant/16-48240041-G-A?dataset=gnomad_r4
[27]
Clinical and Molecular Evidence of ABCC11 Protein Expression in ...
Feb 15, 2017 · In the present study, we demonstrated that ABCC11 538G>A diminishes ABCC11 protein levels in vivo (Figure 1). The expression of ABCC11 mRNA in ...
[28]
The Impact of Natural Selection on an ABCC11 SNP Determining ...
The rs17822931-A allele leading to dry ear- wax in a recessive manner is nearly absent in African pop- ulations, whereas it is found in European populations and ...
[29]
Allele frequencies of the ABCC11 gene for earwax phenotypes ...
Jun 26, 2009 · The ABCC11 gene encodes the multidrug resistance-associated protein 8 that consists of 1382 amino acids and contains 2 ATP-binding domains and ...
[30]
Myths of Human Genetics: Earwax - University of Delaware
Sep 16, 2013 · Yoshiura et al. (2006) then found the gene responsible: ABCC11 (ATP-binding cassette, subfamily C, member 11). The allele for wet earwax has a ...
[31]
rs17822931 RefSNP Report - dbSNP - NCBI
### Summary of rs17822931 Allele Frequencies (1000 Genomes Project)
[32]
Exploring Earwax, Genetics and Human Migration - Ear Health
Jun 9, 2023 · This article will explore what earwax type can tell us about human migration and the ABBCC11 gene mutation that determines your earwax type.What Do Earwax and Early... · The Japanese Earwax Gene...
[33]
Earwax–cerumen genetics and physiology: Current insights...
Research has proposed that a single change in the DNA sequence of the ABCC11 gene leads to the production of dry earwax (Toyoda et al. 2009). In addition to ...
[34]
Estimating genetic load from 5000 Chinese exomes - ScienceDirect
Aug 30, 2025 · ABCC11 rs17822931-A, associated with cold adaptation, is particularly low frequency in G-Han. Gene-based rare-variant collapsing analyses ...
[35]
Population genetic admixture and evolutionary history in the ...
Jun 18, 2024 · ... ABCC11 gene was first identified in ancient Tianyuan individuals. In North America, rs17822931-T appeared approximately 12,000 years ago.Missing: paper | Show results with:paper
[36]
Why many East Asians don't have body odor or need deodorant
Jun 21, 2024 · Between 80 and 95% of East Asians have a dysfunction of the ABCCII gene, which is linked to smelly pits, a number of studies say.Missing: 80-90% AA genotype
[37]
Dependence of deodorant usage on ABCC11 genotype - PubMed
Jan 17, 2013 · Earwax type and axillary odor are genetically determined by rs17822931, a single-nucleotide polymorphism (SNP) located in the ABCC11 gene.Missing: sensory thresholds
[38]
ABCC11 Earwax Trait and Genotype Are Suitable Tools for ...
Aug 13, 2025 · The ABCC11 gene encodes a protein that belongs to the MRP subfamily, characterized by two ATP-binding domains and 12 transmembrane domains [12].Missing: structure | Show results with:structure
[39]
Association between the ABCC11 gene polymorphism-determined ...
Jan 16, 2025 · The ABCC11 gene polymorphism, which determines earwax characteristics, regulates the composition of the ear canal microbiota and its metabolic pathways.