Fact-checked by Grok 2 weeks ago

Steroid

Steroids are a class of organic compounds characterized by a core molecular structure consisting of four fused cycloalkane rings, known as the steroid nucleus or cyclopentaphenanthrene skeleton, often with methyl groups at positions C-10 and C-13 and potentially an alkyl side chain at C-17.^[1] This structure is derived from cholesterol, a fundamental sterol, and steroids encompass a diverse group of polycyclic lipids biochemically related to terpenes.^[2] They play critical roles in biological systems, serving as precursors for hormones, vitamins, and other essential molecules that regulate physiological processes across all vertebrates and many invertebrates.^[3] In biology, steroids are synthesized primarily from cholesterol through enzymatic processes in endocrine glands such as the adrenal cortex, gonads, and placenta.^[4] Key classes include glucocorticoids (e.g., cortisol), which manage stress responses, metabolism, and immune function; mineralocorticoids (e.g., aldosterone), which regulate electrolyte balance and blood pressure; and sex steroids such as androgens (e.g., testosterone) and estrogens, which influence reproduction, secondary sexual characteristics, and development.^[5] Other notable steroids encompass bile acids for fat digestion, vitamin D precursors for calcium homeostasis, and cholesterol itself as a membrane component essential for cell integrity and signaling.^[1] These compounds are lipophilic, allowing them to diffuse across cell membranes and bind intracellular receptors to modulate gene expression.^[6] Medically, steroids have profound applications due to their anti-inflammatory, immunosuppressive, and anabolic properties, though their use requires careful management to mitigate side effects like osteoporosis, hypertension, and hormonal imbalances.^[7] Corticosteroids, synthetic analogs of glucocorticoids, treat conditions including rheumatoid arthritis, asthma, inflammatory bowel disease, and autoimmune disorders by suppressing inflammation and immune overactivity.^[8] Anabolic-androgenic steroids, derived from testosterone, are prescribed for hypogonadism, delayed puberty, muscle wasting in AIDS or cancer, and anemia, but are also misused in sports for performance enhancement, leading to health risks such as cardiovascular disease and liver damage.^[9] The discovery of steroids dates back to the 18th century, with cholesterol first isolated in 1769 and key hormones like testosterone identified in 1935, culminating in Nobel Prize-winning work on cortisone in the 1940s that revolutionized endocrinology and pharmacology.^[10]^[11]

Structure and Nomenclature

Molecular Structure

Steroids are a class of organic compounds defined by a characteristic tetracyclic structure composed of three six-membered cyclohexane rings and one five-membered cyclopentane ring fused in a specific linear arrangement, forming the gonane skeleton.^[1] This core framework, known as cyclopentanoperhydrophenanthrene, consists of 17 carbon atoms in a fully saturated hydrocarbon form, serving as the foundational nucleus for all steroid derivatives.^[1] The carbon atoms in the gonane skeleton are numbered systematically from 1 to 17, starting in ring A and proceeding through the fused system. Ring A encompasses carbons 1 through 5 and 10, ring B includes carbons 5 through 10, ring C covers carbons 8, 9, and 11 through 14, and ring D comprises carbons 13 through 17. The standard stereochemistry features β-orientation of the angular methyl groups attached to C-10 and C-13, contributing to the molecule's three-dimensional rigidity and chirality.^[1]^[12] The basic hydrocarbon skeleton of gonane lacks substituents and exhibits specific ring fusions: A/B sharing the C5–C10 bond, B/C sharing the C8–C9 bond, and C/D sharing the C13–C14 bond, resulting in a compact, planar-like structure with slight curvature. This arrangement can be textually represented as a sequence of fused cycles: cyclohexane (A) fused to cyclohexane (B) trans or cis at A/B, B fused trans to cyclohexane (C), and C fused trans to cyclopentane (D). The rigid, multi-fused ring system minimizes conformational flexibility, enhancing molecular stability.^[12]^[1] The predominantly non-polar, hydrocarbon-based architecture of the gonane skeleton imparts high lipophilicity to steroids, facilitating their passive diffusion across lipid bilayers and biological membranes due to favorable interactions with hydrophobic environments.^[1]^[13]

Functional Groups and Ring Systems

Steroids are characterized by a core tetracyclic ring system modified by various functional groups that dictate their reactivity and physicochemical properties. The most prevalent substituents include hydroxyl groups (-OH), commonly positioned at C-3 (often in the β-orientation) or C-17, and keto groups (=O), frequently at C-3 or C-17 as well. Carbon-carbon double bonds, such as those at \Delta^4 (between C-4 and C-5) or \Delta^5 (between C-5 and C-6), introduce unsaturation primarily in rings A or B. Alkyl side chains, varying in length and structure, are typically attached at C-17; for example, cholesterol features an eight-carbon isooctyl chain at this position, contributing to its role in membrane fluidity.^[14]^[15]^[16] These functional groups profoundly influence the molecule's polarity, solubility, and capacity for hydrogen bonding. Hydroxyl groups enhance polarity by enabling hydrogen bond formation with water, thereby improving aqueous solubility compared to the hydrophobic core; the 3β-hydroxyl in cholesterol, for instance, confers amphipathicity, allowing the molecule to span lipid bilayers with its polar head and nonpolar tail. Keto groups similarly increase polarity but also affect electronic distribution, potentially altering reactivity at adjacent sites. Double bonds reduce saturation, promoting planarity and rigidity in affected rings, while alkyl side chains at C-17 decrease overall polarity, favoring lipid solubility and membrane integration.^[16]^[14]^[15] Variations in the ring system's saturation and stereochemistry further diversify steroid properties. Rings A and B may be fully saturated (as in 5α- or 5β-series) or contain double bonds, impacting conformational flexibility and metabolic susceptibility; for example, \Delta^5 unsaturation in cholesterol supports its fluid incorporation into membranes. The steroid nucleus possesses seven chiral centers at C-5, C-8, C-9, C-10, C-13, C-14, and C-17, with natural steroids exhibiting a conserved stereochemistry of 8β, 9α, 10β, 13β, 14α, and 17β (C-5 varies between α and β). This configuration enforces trans fusions between rings B/C and C/D, and either cis or trans for A/B, resulting in a rigid, slightly curved structure that orients substituents equatorially for minimal steric hindrance.^[15]^[14]^[16] A representative example is the cholestane series, prevalent in vertebrate steroids, where the C-17 eight-carbon side chain—often featuring methyl branches—distinguishes it from shorter-chain variants like androstanes (no side chain) or pregnanes (two-carbon chain), thereby tuning hydrophobicity and biosynthetic pathways.^[16]^[14]

Naming Conventions

Steroid nomenclature follows standardized rules established by the International Union of Pure and Applied Chemistry (IUPAC) and the International Union of Biochemistry and Molecular Biology (IUBMB) to ensure precise and unambiguous identification of these compounds in scientific literature. These rules build upon the characteristic tetracyclic ring system and associated functional groups of steroids, providing a systematic framework for naming based on parent structures, substituents, and stereochemical configurations.^[17] The foundational parent hydrocarbons are derived from the gonane skeleton, which represents the basic tetracyclic structure without angular methyl groups or side chains. Common parent names include estrane (with a methyl group at C-13 but none at C-10 and no C-17 side chain), androstane (methyl groups at both C-10 and C-13, no C-17 side chain), and pregnane (methyl groups at C-10 and C-13, with an acetyl side chain at C-17). Longer side chains lead to names like cholane (eight-carbon chain at C-17) and cholestane (eight-carbon chain with a methyl at C-24). These names are selected based on the length and nature of the side chain at C-17, with numbering starting from the ring junctions and angular methyls at C-18 and C-19.^[17] Functional groups are indicated using standard organic chemistry suffixes and prefixes, integrated into the parent name. Hydroxyl groups (-OH) are denoted by -ol (e.g., for alcohols at position 3), and carbonyl groups (=O) by -one (e.g., for ketones at position 20), with locants specifying positions; multiple groups use multiplicative prefixes like di-, tri-, as in 3,20-dione. Unsaturation is marked by -ene for double bonds, with the locant placed before the suffix (e.g., cholest-5-ene for a double bond between C-5 and C-6), and -adiene for two double bonds. Modifications such as ring reductions are indicated by prefixes like 5α- or 5β- to denote the configuration at the A/B ring junction, reflecting hydrogen addition across the 4-5 double bond in precursors like cholesterol.^[17] Stereochemistry is crucial for distinguishing isomers and is denoted using Greek letters for substituents relative to the ring plane: α for below and β for above, as in 3β-ol. For chiral centers in side chains or complex configurations, the R/S system from Cahn-Ingold-Prelog rules is applied, such as (20R) for the configuration at C-20 in pregnane derivatives. These descriptors ensure that the three-dimensional arrangement, including trans or cis fusions at ring junctions (e.g., 5α-androstane with trans A/B), is clearly conveyed.^[17] The evolution of steroid nomenclature traces back to early 20th-century trivial names, which were descriptive but inconsistent, such as "cholesterol" for the common sterol isolated from gallstones. Initial standardization efforts began with discussions at the 1950 Ciba Foundation Symposium and were formalized in IUPAC's 1952 Zurich proposals, progressing through 1960 Basel amendments and 1965 tentative rules to the 1971 definitive rules, which emphasized systematic substitutive nomenclature over trivial names for clarity. The 1989 revisions further aligned with broader IUPAC organic nomenclature, promoting R/S descriptors and parent hydrocarbon bases while retaining some retained trivial names like testosterone (systematically 17β-hydroxyandrost-4-en-3-one) for common compounds. For instance, cholesterol's trivial name corresponds to the systematic cholest-5-en-3β-ol, illustrating the transition to precise, structure-based naming.^[18]^[17]

Types and Classification

By Biological Function

Steroids are classified by their biological functions into distinct categories that reflect their diverse physiological roles across organisms, ranging from signaling molecules to structural components. This functional classification emphasizes how steroids contribute to key processes such as reproduction, stress response, digestion, membrane integrity, and defense, independent of their precise chemical structures. Hormonal steroids, particularly sex steroids, play critical roles in regulating reproduction and development in vertebrates. Estrogens, such as estradiol, promote female secondary sexual characteristics, ovarian follicle development, and bone maintenance, while androgens like testosterone drive male reproductive organ maturation, spermatogenesis, and muscle growth. Progestogens, including progesterone, support pregnancy by preparing the uterine lining and inhibiting contractions. These hormones are synthesized primarily in the gonads and exert effects through nuclear receptors that modulate gene expression.^[19] Adrenal corticosteroids represent another major functional class, divided into glucocorticoids and mineralocorticoids, which are essential for metabolic and homeostatic regulation in mammals. Glucocorticoids, exemplified by cortisol, mediate the stress response by increasing glucose availability, suppressing inflammation, and modulating immune function during acute challenges. Mineralocorticoids, such as aldosterone, maintain electrolyte balance and blood pressure by promoting sodium reabsorption in the kidneys via mineralocorticoid receptors. These steroids are produced in the adrenal cortex and are vital for survival under physiological stress.^[19] Bile acids, derived from cholesterol, function primarily as digestive aids in vertebrates by emulsifying dietary fats and facilitating their absorption in the intestine. They also serve as signaling molecules that regulate glucose and lipid metabolism through receptors like FXR, while cholesterol itself acts as a key membrane component, modulating fluidity and permeability in animal cells. These roles highlight the transition of cholesterol-derived steroids from structural to active physiological agents.^[20]^[21] In plants, phytosterols such as sitosterol and campesterol contribute to membrane stability by maintaining fluidity and permeability under varying environmental conditions, and they serve as precursors for signaling molecules like brassinosteroids that influence growth and stress responses. Similarly, in fungi, mycosterols like ergosterol ensure cell membrane integrity, regulate permeability, and support plasma membrane biogenesis, which are crucial for fungal viability and adaptation.^[22] Certain modified steroids, such as steroidal saponins and glycoalkaloids, function as defense compounds in plants, deterring herbivores and pathogens through their toxicity and ability to disrupt cell membranes. For instance, compounds like α-tomatine in tomatoes provide chemical barriers against insect pests and microbial invaders.^[23]^[24]

By Chemical Structure

Steroids are classified by chemical structure primarily according to variations in their tetracyclic core skeleton, the presence and type of functional groups, side chain modifications, and overall carbon atom count, which reflect evolutionary adaptations and biosynthetic divergences across organisms.^[25] This structural taxonomy distinguishes major families such as sterols, bile acids, cardiac glycosides, and secosteroids, each characterized by specific substituent patterns that influence solubility, reactivity, and biological interactions.^[26] Sterols represent a fundamental class of steroids featuring an intact four-ring perhydro-1,2-cyclopentanophenanthrene nucleus with a hydroxyl group typically at the C-3 position, often accompanied by an eight-carbon side chain at C-17, resulting in a C27 to C29 carbon framework.^[26] Cholesterol, the predominant sterol in animal cells, exemplifies this structure with its double bond between C-5 and C-6 and a branched isooctyl side chain, serving as a membrane component and precursor to other steroids.^[26] In fungi, ergosterol mirrors cholesterol but includes additional methyl groups and a different side chain configuration, enabling distinct membrane fluidity properties.^[22] Bile acids derive from sterols through oxidative modifications, featuring a shortened side chain at C-17 reduced to five carbons with a terminal carboxyl group, yielding a C24 skeleton, alongside hydroxyl groups on the A/B rings for enhanced amphiphilicity.^[26] Cholic acid, with three hydroxyl groups at C-3, C-7, and C-12, illustrates this class, formed via cholesterol catabolism in the liver to facilitate lipid digestion in vertebrates.^[26] These derivatives often conjugate with glycine or taurine, further altering their polarity for enterohepatic circulation.^[25] Cardenolides and bufadienolides constitute cardiac glycoside subclasses distinguished by lactone rings fused to the steroid core at C-17, imparting potent bioactivity despite their shared tetracyclic base.^[25] Cardenolides possess a five-membered unsaturated butyrolactone ring, resulting in a C23 framework, as seen in digitoxin from foxglove plants, which inhibits Na+/K+-ATPase in cardiac tissue.^[27] Bufadienolides, conversely, feature a six-membered α-pyrone lactone with two double bonds, extending to C24, exemplified by bufalin from toad venom, noted for its cytotoxicity and potential anticancer effects.^[28] Secosteroids are characterized by cleavage of one or more rings in the core structure, most notably the opening of the B-ring via breakage of the 9,10-carbon bond, transforming the typical cyclopentanophenanthrene into an open-chain variant while retaining steroid-like functionality.^[25] Vitamin D3 (cholecalciferol), derived from 7-dehydrocholesterol, embodies this class with its triene system and preserved side chain, essential for calcium homeostasis in vertebrates.^[26] A key structural distinction within steroid hormones arises from variations in carbon atom count, which stem from side chain alterations or aromatization of the A-ring.^[29] C19 androgens, such as testosterone based on the androstane skeleton, feature a methyl group at C-17 without an extended chain; C21 progestogens and corticosteroids, like progesterone and cortisol on the pregnane skeleton, include a two-carbon acetyl side chain at C-17; while C18 estrogens, such as estradiol on the estrane skeleton, undergo A-ring aromatization and loss of the C-19 methyl group.^[30] These differences dictate receptor specificity and physiological roles, such as reproductive regulation for estrogens and stress response for corticosteroids.^[31]

Intact Versus Modified Ring Systems

Steroids with intact ring systems feature the canonical gonane nucleus, a tetracyclic structure comprising three fused six-membered rings (A, B, and C) and one five-membered ring (D), providing a rigid scaffold essential for their biological roles. This configuration is characteristic of most naturally occurring steroids in eukaryotes, such as cholesterol, which serves as a membrane component and biosynthetic precursor, and hormones like testosterone and cortisol, which rely on the fused rings for specific receptor interactions. The stability of this intact system arises from the trans fusions at key junctions (A/B and C/D), minimizing conformational flexibility while enabling efficient membrane permeation and enzymatic processing.^[32] In contrast, cleaved ring systems represent a major modification where one ring bond is broken, resulting in secosteroids with increased molecular flexibility. Vitamin D3 (cholecalciferol), for example, arises from 7-dehydrocholesterol via ultraviolet-induced photochemical cleavage of the B ring (between carbons 9 and 10), transforming the closed tetracycle into an open-chain triene system. This alteration allows vitamin D3 to adopt extended conformations that facilitate binding to the vitamin D receptor, regulating gene expression for calcium absorption, unlike the more rigid intact steroids.^[33] Contracted ring systems involve the removal of a methylene group from one ring, often denoted by the "nor" prefix, leading to rare variants with altered steric properties. B-norsteroids, such as those isolated from certain marine sponges, exemplify this contraction in the B ring, reducing the six-membered ring to five members and shifting the fusion geometry. These modifications are uncommon in nature but occur in specialized biosynthetic pathways, potentially enhancing compactness for targeted antimicrobial or cytotoxic functions in producer organisms.^[29] Expanded ring systems, indicated by the "homo" prefix, incorporate an additional carbon atom into a ring, though natural examples are scarce and often limited to synthetic analogs. Brassinosteroids, plant growth regulators like brassinolide, maintain an intact tetracyclic core but feature ring expansions through side-chain extensions at C-17 and B-ring lactonization (e.g., a seven-membered oxolactone fusing C-6 and C-7), which modulates their interaction with the BRI1 receptor kinase. This structural adjustment increases hydrophilicity and binding specificity compared to standard steroids.^[34] Such ring modifications profoundly influence steroid chemistry and biology by altering molecular dynamics and interactions. Cleavage enhances rotational freedom, improving solubility and receptor docking as seen in vitamin D's nuclear signaling, while contraction or expansion adjusts rigidity and polarity, potentially boosting metabolic resistance or selectivity—for instance, D-ring opening in androstenedione analogs reduces aromatase inhibition affinity by over 300-fold due to loss of cyclopentane constraints. These changes underscore how ring integrity dictates flexibility for transport, binding affinity to steroid receptors, and enzymatic susceptibility in pathways like steroidogenesis.^[35]

Distribution Across Organisms

In Eukaryotes

Sterols are ubiquitous components of eukaryotic cell membranes, where they play a critical role in maintaining membrane fluidity and organization. In animals, cholesterol predominates as the primary sterol, constituting a significant portion of plasma membrane lipids and modulating the packing of phospholipids to ensure optimal membrane dynamics.^[36] In fungi, ergosterol serves a analogous function, similarly regulating membrane fluidity by ordering lipid acyl chains while preventing excessive rigidity.^[37] This presence of sterols is a defining feature of eukaryotic membranes, contrasting with prokaryotes that typically rely on simpler hopanoids for similar purposes.^[38] The biosynthesis of sterols exhibits remarkable evolutionary conservation across eukaryotes, tracing back to their last common ancestor and reflecting an ancient adaptation for membrane stabilization. Genes involved in the cholesterol biosynthesis pathway, such as those encoding squalene monooxygenase and lanosterol synthase, are highly preserved from yeast to humans, underscoring the pathway's essentiality for eukaryotic cellular integrity.^[39] This conservation highlights sterols' role in enabling the complex membrane architectures required for eukaryotic multicellularity and compartmentalization.^[40] Variations in sterol composition occur across eukaryotic kingdoms, adapting to specific environmental and physiological needs. In plants, phytosterols such as β-sitosterol and stigmasterol are predominant, with β-sitosterol being the most abundant and contributing to membrane stability under varying osmotic conditions.^[41] Stigmasterol, often found in high levels in soybeans and other oilseeds, further diversifies plant membrane sterols, influencing growth and stress responses.^[42] Quantitatively, sterols can comprise 30-40% of total lipids in eukaryotic plasma membranes, such as in yeast and mammalian cells, ensuring structural resilience and functional versatility.^[43] A unique feature of eukaryotic sterols, particularly cholesterol, is their involvement in forming lipid rafts—specialized, ordered microdomains that facilitate protein clustering and signaling. Cholesterol enriches these rafts by interacting with sphingolipids, promoting phase separation and enhancing membrane protein mobility in a controlled manner.^[44] This property is integral to eukaryotic cellular processes, distinguishing them from the more uniform membrane organization in prokaryotes.^[45]

In Prokaryotes

True steroids, characterized by their tetracyclic structure derived from oxidosqualene, are rare in prokaryotes, which predominantly rely on hopanoids—pentacyclic triterpenoids—as structural and functional analogs for maintaining membrane fluidity and stability.^[46] Hopanoids, synthesized via squalene cyclization without oxygen dependence, mimic the rigidifying effects of eukaryotic sterols like cholesterol, preventing phase separation in bacterial lipid bilayers under varying environmental stresses. This reliance on hopanoids underscores the prokaryotic adaptation to diverse habitats, where true steroids would require oxygen-dependent enzymes absent in most lineages.^[47] Exceptions to this rarity occur in select bacteria capable of de novo steroid biosynthesis, often through horizontal gene transfer of eukaryotic-like pathways. For instance, the aerobic methanotroph Methylococcus capsulatus produces cholesterol and C-4 methylated sterols, such as 4α-methylzymosterol, primarily localizing to the outer membrane to enhance rigidity in oxygen-rich environments.^[48] Similarly, myxobacteria like Enhygromyxa salina synthesize cholesterol via the Bloch pathway, involving oxidosqualene cyclase and squalene monooxygenase, with these sterols sometimes conjugated to other lipids for specialized functions.^[49] In archaea, including methanogens, complete steroid biosynthetic machinery is absent, though trace steroidal compounds may appear as minor environmental acquisitions rather than endogenous products.^[50] Evolutionarily, steroid biosynthesis originated in prokaryotes, particularly bacteria, around the Great Oxidation Event approximately 2.4 billion years ago, evolving from hopanoid precursors to support aerobic adaptations before horizontal transfer to eukaryotes.^[50] This transfer likely involved genes from alphaproteobacteria or myxobacteria, enabling the diversification of sterol roles in eukaryotic membranes. Advanced detection methods, such as gas chromatography-mass spectrometry (GC-MS) and lipidomics profiling, have confirmed the low abundance of these steroids in prokaryotes, typically comprising less than 1% of total membrane lipids even in producing species.^[51]

In Fungi, Plants, and Animals

In fungi, ergosterol serves as the primary sterol, comprising the main component of cell membranes where it maintains fluidity and permeability, and it acts as a key precursor for vitamin D2 (ergocalciferol) upon ultraviolet irradiation.^[52]^[53] Certain fungal species, particularly within genera like Tuber and Terfezia, also produce significant variants such as brassicasterol, which can constitute up to 98% of total sterols in some truffles and contributes to membrane stability in diverse phylogenetic groups across 175 fungal species.^[54]^[55] Plants synthesize a variety of phytosterols that support membrane integrity and growth processes; for instance, β-sitosterol is a predominant phytosterol that regulates key metabolites involved in rice seedling growth and enhances tolerance to environmental stresses by modulating cellular signaling pathways.^[56] Additionally, brassinosteroids function as essential plant hormones that promote cell elongation and division, influencing overall plant height and development through interactions with gibberellin pathways and cell wall loosening mechanisms.^[57] In animals, cholesterol is the dominant sterol, essential for membrane structure and serving as a precursor for specialized derivatives across phyla. In insects, cholesterol is converted into ecdysteroids like ecdysone, which regulate molting and metamorphosis by acting on the prothoracic glands.^[58] In vertebrates, cholesterol is further metabolized into bile acids, which facilitate lipid digestion and absorption in the intestine, with ecdysteroid analogs potentially influencing this pathway in experimental models.^[59]^[60] Comparative analyses reveal distinct abundance patterns: plants typically produce phytosterols at levels of 100-400 mg per kg of fresh weight, varying by species and tissue, while animals exhibit higher turnover rates, with cholesterol processing reaching up to 2 g per day in larger mammals to maintain homeostasis amid dietary intake and excretion.^[61]^[62] Environmental adaptations are evident in aquatic ecosystems, where algal sterols—such as those in microalgae—undergo diverse modifications driven by ecological pressures like light and pH, enabling survival in dynamic habitats through enhanced membrane resilience and symbiotic interactions.^[63]^[64]

Biological and Physiological Roles

General Significance

Steroids play a fundamental role in maintaining cell membrane integrity across eukaryotic organisms by modulating membrane fluidity and preventing phase transitions between liquid-ordered and liquid-disordered states. Cholesterol, the most prevalent sterol in animal cells, intercalates between phospholipid molecules, reducing membrane permeability and stabilizing bilayers against mechanical stress and temperature fluctuations. This structural function is essential for cellular homeostasis, as disruptions in sterol content can lead to membrane instability and impaired cellular function.^[65] In addition to their structural contributions, steroids serve as critical signaling molecules, particularly as hormones that regulate gene expression through nuclear receptors. Steroid hormones, such as glucocorticoids and sex steroids, diffuse across cell membranes and bind to intracellular nuclear receptors, forming complexes that translocate to the nucleus and modulate transcription of target genes involved in metabolism, reproduction, and immune response. This ligand-activated mechanism allows steroids to coordinate physiological processes at a genomic level, influencing development and adaptation in multicellular organisms.^[66] The presence of steroids marks a key evolutionary milestone, distinguishing eukaryotic lineages and facilitating adaptations to oxygenated environments. Steroid biosynthesis likely emerged in early eukaryotes as a response to rising atmospheric oxygen, enabling the endosymbiotic acquisition of mitochondria and enhancing membrane rigidity for aerobic metabolism. Fossil evidence of ancient steranes suggests steroids were integral to the diversification of complex life forms over 1.6 billion years ago.^[67] Imbalances in steroid levels have profound health implications; deficiencies, such as hypocholesterolemia, are associated with increased risks of hepatic complications, depression, and higher mortality in critical conditions due to compromised membrane function and hormone production.^[68]^[69]^[70] Conversely, excesses, particularly elevated cholesterol, contribute to disorders like atherosclerosis by promoting lipid accumulation in arterial walls, leading to cardiovascular disease.^[71] These impacts underscore the tight regulation required for steroid homeostasis in human physiology. Ecologically, sterols from primary producers like algae are vital in aquatic food chains, serving as essential nutrients for higher trophic levels including zooplankton and marine animals that cannot synthesize them de novo. These sterols support membrane integrity in consumers, influencing growth, reproduction, and population dynamics; for instance, sterol limitation in herbivores can cascade through ecosystems, affecting biodiversity and energy transfer.^[72]

Specific Functions in Key Organisms

In animals, glucocorticoids such as cortisol play a central role in regulating metabolism by promoting gluconeogenesis, suppressing immune responses, and mobilizing energy stores during stress, with plasma cortisol levels increasing approximately 9-fold in response to acute stressors in healthy young men.^[73] Estrogens and androgens function primarily in reproduction, where estrogens support female reproductive tract development, ovulation, and secondary sexual characteristics, while androgens drive spermatogenesis, prostate function, and male reproductive system maturation.^[74] In arthropods, ecdysteroids like ecdysone orchestrate developmental processes, including molting, metamorphosis, and reproductive maturation, by binding to nuclear receptors that trigger cascades of gene expression essential for growth transitions.^[75] In plants, brassinosteroids act as key regulators of vascular differentiation by promoting xylem and phloem cell elongation and division, thereby influencing stem growth and overall architecture.^[76] These hormones also enhance stress responses, such as tolerance to drought and temperature extremes, through modulation of antioxidant enzyme activity and osmotic adjustment mechanisms.^[77] Phytosterols contribute to pathogen defense by altering membrane composition to limit nutrient efflux into the apoplast, thereby inhibiting bacterial proliferation and bolstering innate immunity against infections.^[78] In fungi, ergosterol serves as a critical membrane component that maintains fluidity and permeability, supporting hyphal growth and extension necessary for nutrient uptake and colony expansion.^[22] Its biosynthesis is a prime antifungal target, as azole drugs inhibit the enzyme lanosterol 14α-demethylase, disrupting ergosterol production and leading to membrane instability and fungal cell death.^[79] Steroids exhibit inter-organismal interactions, notably where plant sterols like sitosterol and campesterol reduce human cholesterol absorption in the intestine by competing for uptake via the NPC1L1 transporter, lowering serum LDL-cholesterol levels by up to 10% with daily intakes of 2-3 grams.^[80]

Biosynthesis and Metabolism

Mevalonate Pathway

The mevalonate pathway represents the primary route for the biosynthesis of isoprenoid precursors in steroid production, initiating from acetyl-coenzyme A (acetyl-CoA) and culminating in the formation of isopentenyl pyrophosphate (IPP) and its isomer dimethylallyl pyrophosphate (DMAPP), which serve as building blocks for sterol synthesis such as cholesterol.^[81] This anabolic sequence occurs predominantly in the cytosol and endoplasmic reticulum of eukaryotic cells, where it supports the production of steroids essential for membrane integrity, hormone signaling, and other cellular functions.^[82] The pathway commences with the condensation of two molecules of acetyl-CoA to form acetoacetyl-CoA, catalyzed by acetoacetyl-CoA thiolase (EC 2.3.1.9).^[81] This intermediate then reacts with a third acetyl-CoA molecule in the presence of HMG-CoA synthase (EC 2.3.3.10) to yield 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA).^[81] The subsequent reduction of HMG-CoA to mevalonate is mediated by HMG-CoA reductase (EC 1.1.1.34), the rate-limiting enzyme of the pathway, which utilizes two molecules of NADPH as cofactors in a two-step process involving an aldehyde intermediate.^[81] Mevalonate is then sequentially phosphorylated by mevalonate kinase (EC 2.7.1.36) to mevalonate-5-phosphate, using one ATP, followed by further phosphorylation by phosphomevalonate kinase (EC 2.7.4.2) to mevalonate-5-diphosphate, consuming another ATP.^[81] The final activation step involves decarboxylation of mevalonate-5-diphosphate by mevalonate diphosphate decarboxylase (EC 4.1.1.33), which requires ATP and produces IPP along with CO₂ and phosphate.^[81] IPP is then isomerized to DMAPP by isopentenyl-diphosphate delta-isomerase (EC 5.3.3.2), enabling the formation of longer prenyl chains that lead to sterol precursors.^[81] The net stoichiometry for producing one IPP unit from three acetyl-CoA molecules through these six enzymatic steps is as follows:

$3 \ acetyl\text{-CoA} + 3 \ ATP + 2 \ NADPH \rightarrow IPP + 3 \ ADP + 3 \ P_i + 2 \ NADP^+ + 3 \ CoA + CO_2 + H_2O

This equation highlights the energy investment required, with three ATP molecules hydrolyzed and two NADPH equivalents consumed, underscoring the pathway's metabolic cost. Regulation of the mevalonate pathway centers on HMG-CoA reductase, which is subject to multifaceted control including transcriptional activation by sterol regulatory element-binding protein 2 (SREBP-2) under low sterol conditions, as well as posttranslational mechanisms such as phosphorylation and ubiquitination for degradation.^[82] Feedback inhibition occurs when downstream sterols like cholesterol accumulate, suppressing reductase activity through sterol-sensing proteins that promote enzyme degradation.^[81] Pharmacological inhibitors known as statins competitively bind the HMG-CoA reductase active site, mimicking the substrate and reducing sterol synthesis, which has therapeutic implications for hypercholesterolemia.^[81] Additional feedback at downstream steps, such as inhibition of mevalonate kinase by geranyl diphosphate, further fine-tunes flux toward steroid precursors.^[81] This pathway is nearly universal among eukaryotes, where it drives endogenous sterol production, and is also present in some prokaryotes, including certain eubacteria and archaea, though with variations in enzyme isoforms and regulatory elements.^[81]

Steroidogenesis Process

Steroidogenesis begins with the synthesis of squalene from isoprenoid precursors derived from the mevalonate pathway. Isopentenyl pyrophosphate (IPP) and its isomer dimethylallyl pyrophosphate (DMAPP) undergo sequential head-to-tail condensations catalyzed by farnesyl pyrophosphate synthase to form the 15-carbon farnesyl pyrophosphate (FPP).^[83] Two molecules of FPP are then joined in a head-to-head manner by squalene synthase, which utilizes NADPH as a cofactor, to produce the linear 30-carbon squalene molecule.^[84] The next critical phase involves the cyclization of squalene to form the tetracyclic sterol core. Squalene is first oxidized at the 2,3-position by squalene epoxidase to yield 2,3-oxidosqualene.^[85] This epoxide then undergoes a complex polycyclization reaction catalyzed by oxidosqualene cyclase, resulting in the formation of lanosterol in animals and fungi or cycloartenol in plants.^[86] The cyclase enzyme facilitates a series of carbocation rearrangements and ring closures, establishing the characteristic four-ring structure of steroids.^[40] Maturation of lanosterol to cholesterol requires a series of demethylation, isomerization, and reduction steps, totaling 19 enzymatic reactions in animals.^[86] These include oxidative removal of methyl groups at C14 and C4 positions by cytochrome P450 enzymes, migration of double bonds (e.g., from Δ8 to Δ7), and saturation of the side chain via sterol Δ24-reductase, which reduces the Δ24 double bond using NADPH.^[87] The process eliminates three carbon atoms as CO2 and formate, refining the sterol nucleus and side chain.^[86] The overall stoichiometry of cholesterol biosynthesis from acetyl-CoA highlights its energetic cost:

$18 \text{ acetyl-CoA} + 18 \text{ ATP} + 16 \text{ NADPH} \rightarrow 1 \text{ cholesterol} + 9 \text{ CO}_2 + 18 \text{ ADP} + 18 \text{ P}_i + 16 \text{ NADP}^+ + 11 \text{ H}_2\text{O}

This equation encompasses the conversion through squalene and lanosterol intermediates.^[88] In specialized tissues such as the gonads, steroidogenesis proceeds from cholesterol as the substrate to produce steroid hormones. Cholesterol is transported into mitochondria and cleaved by the cytochrome P450 enzyme CYP11A1 (also known as cholesterol side-chain cleavage enzyme) to form pregnenolone, the precursor for all other steroids.^[89] This rate-limiting step involves three sequential oxidations and decarboxylation, releasing isocaproic aldehyde.^[90]

Alternative Biosynthetic Routes

In addition to the canonical mevalonate pathway, the methylerythritol phosphate (MEP) pathway serves as an alternative route for synthesizing isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP), the universal precursors for isoprenoids including steroids, in plastids of plants and in many bacteria.^[91] This pathway initiates with the condensation of glyceraldehyde-3-phosphate and pyruvate to form 1-deoxy-D-xylulose 5-phosphate (DXP), followed by a series of seven enzymatic steps that bypass mevalonate entirely, producing IPP and DMAPP.^[92] The MEP pathway is particularly prominent in prokaryotes and plant plastids, where it supports the biosynthesis of sterols and other terpenoids essential for cellular functions.^[93] In plants, crosstalk between the cytosolic mevalonate pathway and the plastidial MEP pathway enables hybrid contributions to sterol supply, allowing IPP to be exchanged across compartments to meet demands for cholesterol and phytosterol production.^[94] For instance, inhibition of the mevalonate pathway can redirect flux through the MEP route, as demonstrated in tobacco cells where labeled precursors confirmed MEP-derived IPP incorporation into sterols.^[95] This metabolic flexibility ensures robust sterol biosynthesis under varying physiological conditions, such as stress or developmental stages.^[96] Microbial variants of steroid biosynthesis often leverage the MEP pathway, with some bacteria naturally producing sterols like cholesterol using endogenous enzymes, while engineered strains incorporate eukaryotic genes for enhanced output.^[50] For example, Escherichia coli has been modified by introducing yeast oxidosqualene cyclase and other eukaryotic genes to enable de novo lanosterol synthesis from MEP-derived precursors, yielding up to 20 mg/L in optimized cultures.^[97] Recent advances as of 2025 include electron transfer engineering in microbial cell factories to further improve steroid production efficiency.^[98] Such engineered pathways highlight the adaptability of bacterial systems for industrial steroid production.^[99] Evolutionarily, the MEP pathway is considered ancient, predating the mevalonate pathway and originating in early prokaryotes as the primary route for isoprenoid synthesis before the divergence of archaea and eukaryotes.^[92] This primacy is evidenced by its widespread distribution in bacteria and its retention in plastids, which trace back to cyanobacterial endosymbionts.^[100] A notable example is the biosynthesis of artemisinin precursors in Artemisia annua, where the MEP pathway predominantly supplies IPP for amorpha-4,11-diene, the sesquiterpene intermediate leading to the antimalarial compound.^[101]

Catabolism, Excretion, and Analysis

Metabolic Breakdown and Excretion

Steroids undergo metabolic breakdown primarily in the liver and other peripheral tissues to inactivate them and facilitate their elimination, thereby maintaining physiological homeostasis. This catabolism involves phase I reactions such as hydroxylation and reduction, followed by phase II conjugation to enhance water solubility. Cytochrome P450 enzymes, including CYP3A4, catalyze key hydroxylation steps that introduce hydroxyl groups, increasing the polarity of steroids like cortisol and androgens.^[102] Reductions, mediated by enzymes such as 5α-reductase (SRD5A) and 3α-hydroxysteroid dehydrogenases (AKR1C), further modify the steroid structure, often inactivating hormones like testosterone to dihydrotestosterone derivatives.^[102] A major catabolic route for cholesterol, the precursor steroid, is its conversion to bile acids, which represents the primary pathway for sterol elimination. This process begins with 7α-hydroxylation of cholesterol by cholesterol 7α-hydroxylase (CYP7A1) in the classic pathway, leading to intermediates that undergo further modifications, including side-chain oxidation and cleavage to shorten the 8-carbon side chain to a 5-carbon one. The resulting primary bile acids are cholic acid (CA) and chenodeoxycholic acid (CDCA), formed via additional hydroxylations and oxidations.^[103] Conjugation of these bile acids with glycine or taurine produces bile salts, which are secreted into bile for lipid emulsification in the intestine.^[103] The simplified pathway for chenodeoxycholic acid formation can be represented as:

\text{Cholesterol} \xrightarrow{\text{CYP7A1 (7α-hydroxylation)}} 7\alpha\text{-hydroxycholesterol} \rightarrow \cdots \rightarrow \text{Chenodeoxycholic acid (CDCA)} \rightarrow \text{Bile salts (conjugated CDCA)}

This equation highlights the initial rate-limiting 7α-hydroxylation step.^[103] Following catabolism, steroids and their metabolites are excreted mainly via urine and feces. Urinary excretion accounts for the majority of eliminated steroid hormones, with approximately 80% as water-soluble conjugates like glucuronides (via UGT2B7 and UGT1A10) or sulfates (via SULT2A1), including estrogen metabolites and tetrahydrocortisol.^[102] Fecal excretion predominates for bile acids, where about 95% are reabsorbed in the ileum through enterohepatic circulation—secreted into bile, delivered to the gut, and returned to the liver via the portal vein—while the remaining 5% is lost in feces, representing the main route of cholesterol disposal.^[103] Gut microbiota influence this process by deconjugating and modifying bile acids, such as through 7α-dehydroxylation to form secondary bile acids like deoxycholic acid. Impaired metabolic breakdown or excretion can lead to pathological accumulation. In cholestasis, disrupted bile flow hinders enterohepatic circulation and biliary secretion, causing buildup of bile acids and other steroids in the liver and serum, which contributes to hepatocyte injury, inflammation, and pruritus.^[104] Conditions like intrahepatic cholestasis of pregnancy exemplify this, where elevated estrogen levels exacerbate impaired sulfation and transport of bile acids.^[103]

Isolation Techniques

Steroids are typically isolated from natural sources such as animal tissues, plant materials, and microbial cultures through a series of extraction, separation, and purification steps designed to exploit their lipophilic nature and structural similarities to other lipids. Initial extraction often employs organic solvents to dissolve steroids from complex biological matrices, with the Folch method—using a 2:1 mixture of chloroform and methanol—being a widely adopted technique for isolating lipids including steroids from tissues like adrenal glands or plant leaves, as it effectively partitions non-polar compounds while minimizing aqueous interference.^[105]^[106] For plant-derived sterols such as β-sitosterol, supercritical fluid extraction with CO₂ under pressures of 200–400 bar and temperatures of 40–60°C provides an environmentally friendly alternative, yielding extracts enriched in phytosterols with minimal solvent residues and high selectivity for non-polar components.^[107]^[108] Following extraction, separation techniques leverage differences in polarity, solubility, and molecular interactions to purify steroids from co-extracted lipids and impurities. Thin-layer chromatography (TLC) on silica gel plates, often with mobile phases like chloroform-methanol-water or hexane-ethyl acetate, is commonly used for preliminary separation of steroids based on polarity; for instance, bile acids such as cholic acid and deoxycholic acid can be resolved on silica gel using reversed-phase systems, allowing visualization under UV light or with spraying agents like phosphomolybdic acid.^[109]^[110] High-performance liquid chromatography (HPLC), particularly reversed-phase variants with C18 columns and gradients of acetonitrile-water or methanol-water, offers higher resolution for complex mixtures, enabling the separation of bile acid derivatives by their hydrophilic side chains while achieving baseline resolution for structurally similar compounds.^[111]^[112] Purification often concludes with precipitation and crystallization to obtain high-purity steroids, especially from animal sources. In the isolation of cortisol from bovine adrenal glands, tissues are homogenized, extracted with solvents like petroleum ether or acetone, and the crude steroid fraction precipitated by cooling or pH adjustment, followed by recrystallization from ethanol or methanol to yield crystalline material with purity exceeding 95%.^[113] Historically, in the 1930s, Edward C. Kendall's group at the Mayo Clinic extracted adrenal cortex lipids using benzene and alcohol, followed by fractional precipitation with digitonin to selectively bind cholesterol-like steroids and subsequent crystallization, which enabled the isolation of cortisone (Compound E) in milligram quantities from tons of glandular material.^[114]^[115] Yield considerations are critical in steroid isolation, as natural abundances vary widely; for example, cholesterol extraction from wool grease typically yields 10–15% of the starting material after saponification of esters and solvent partitioning with petroleum ether, followed by chromatography or distillation to remove accompanying sterols like lanosterol.^[116]^[117] These methods ensure scalable procurement while preserving steroid integrity, though overall recoveries depend on source quality and processing efficiency, often ranging from 50–80% for optimized protocols.^[118]

Structure Determination and Analytical Methods

The determination of steroid structures relies on a suite of analytical techniques that elucidate molecular architecture, stereochemistry, and functional group composition, often applied post-isolation from biological matrices. These methods have evolved from classical spectroscopic and crystallographic approaches to advanced hyphenated systems, enabling precise characterization essential for understanding steroid diversity and function. Infrared (IR) spectroscopy serves as a foundational tool for identifying functional groups in steroids, with characteristic absorption bands indicating the presence of carbonyls, hydroxyls, and olefins. For instance, ketone carbonyls in steroids typically absorb around 1700 cm⁻¹, while conjugated systems shift to lower wavenumbers near 1660-1680 cm⁻¹, allowing differentiation of structural motifs like Δ⁴-3-keto groups in corticosteroids.^[119] Hydroxyl groups exhibit broad O-H stretches at 3200-3600 cm⁻¹, and C=C stretches appear at 1640-1680 cm⁻¹, providing rapid qualitative assessment without sample destruction.^[120] These spectral features, correlated with steroid substitution patterns, were systematically reviewed in early applications, aiding in the verification of synthetic derivatives and natural isolates.^[121] Nuclear magnetic resonance (NMR) spectroscopy excels in resolving the stereochemistry and proton environments within the steroid nucleus, particularly through ¹H and ¹³C NMR shifts for ring protons and carbons. Axial and equatorial protons in the A/B rings of cholesterol derivatives, for example, display distinct chemical shifts (e.g., ¹H at 0.6-2.5 ppm for methylene groups), enabling assignment of cis/trans fusions via coupling constants (J values of 4-12 Hz).^[122] Multidimensional techniques like COSY, HSQC, and NOESY further delineate side-chain configurations and stereocenters, as demonstrated in the complete ¹H assignment of diosgenin benzoate using high-field (750 MHz) spectra.^[123] For complex steroidal saponins, systematic 2D NMR approaches integrate scalar and NOE correlations to confirm glycosidic linkages and aglycone structures.^[124] Mass spectrometry, particularly electron ionization mass spectrometry (EI-MS), provides molecular weight confirmation and fragmentation insights into steroid skeletons. The molecular ion peak yields the exact mass, while characteristic fragments reveal ring cleavages; for androgens, loss of the C-17 side chain often produces a prominent ion at m/z 255 from retro-Diels-Alder fission of ring B/C.^[125] Deuterium-labeled analogs have mapped these pathways, showing common losses like water (m/z 18) from hydroxyls or methyl groups (m/z 15) from angular substituents, facilitating structural elucidation of metabolites.^[126] Low-energy EI variants optimize molecular ion abundance for trace analysis, minimizing excessive fragmentation in steroidomics workflows.^[127] X-ray crystallography delivers absolute configurations by resolving three-dimensional atomic arrangements in crystalline steroid derivatives. In the 1940s, Dorothy Hodgkin used X-ray analysis to confirm the structure of cholesterol iodide, establishing the β-configuration at C-3 and overall tetracyclic framework, which informed broader steroid stereochemistry.^[128] Early surveys of over 80 sterol crystals in the 1940s correlated unit cell dimensions with functional group positions, laying groundwork for modern applications in resolving ambiguous chiral centers.^[129] Chromatographic methods coupled with mass spectrometry, such as gas chromatography-mass spectrometry (GC-MS), enable quantification and structural verification of steroids in plasma following derivatization to enhance volatility. GC-MS profiles up to 20 endogenous steroids with limits of detection in the ng/mL range, separating isobaric compounds like 11-deoxycortisol via retention times and MS confirmation.^[130] This approach excels in clinical steroidomics, integrating fragmentation data for unambiguous identification in complex matrices.^[131] Recent advances in liquid chromatography-tandem mass spectrometry (LC-MS/MS) have improved sensitivity for trace steroid metabolites, achieving sub-pg/mL detection without derivatization through multiple reaction monitoring.^[132] Differential mobility spectrometry enhances LC-MS/MS selectivity, resolving isomeric steroids like testosterone epimers in serum.^[133] Emerging AI-assisted NMR tools accelerate peak assignments in complex spectra, though applications to steroids remain exploratory, building on machine learning for biomolecular structure prediction.^[134]

Chemical Synthesis

Precursor Materials

In the chemical synthesis of steroids, precursor materials are selected based on their structural resemblance to target molecules, availability in large quantities, and economic viability, which facilitate efficient semisynthetic transformations.^[135] Natural plant-derived compounds have become predominant due to their abundance and sustainability advantages over earlier animal sources.^[136] Diosgenin, a steroidal sapogenin extracted from the tubers of Dioscorea species such as yams, serves as a primary natural precursor for industrial steroid production. It is hydrolyzed from saponins in plant material and provides a spiroketal side chain that can be readily converted into key intermediates like 16-dehydropregnenolone acetate, enabling the synthesis of corticosteroids, progestins, and androgens such as cortisone, progesterone, and pregnenolone.^[137]^[138] Stigmasterol, a phytosterol isolated from soybean oil, is another widely used plant-based precursor, particularly valued for its double bond in the side chain that supports the production of progesterone and related hormones through oxidative cleavage and reduction steps.^[139] Microbial fermentation offers an alternative source of precursors, leveraging bacteria to convert inexpensive phytosterols into valuable steroid intermediates such as testosterone. Strains of Mycolicibacterium, such as M. neoaurum, are engineered to degrade the side chains of plant sterols like sitosterol or campesterol, yielding testosterone with high yields up to >66% molar conversion in optimized processes.^[140] This approach enhances sustainability by utilizing renewable plant feedstocks and reducing reliance on direct extraction methods.^[136] Historically, steroid synthesis relied on animal-derived precursors, including bile acids like cholic acid obtained from ox bile and cholesterol extracted from slaughterhouse byproducts such as spinal cords or adipose tissues. In the 1940s, these materials were the sole sources for producing cortisone, requiring laborious isolation from thousands of animal gallbladders to meet clinical demands during early arthritis treatments.^[141] The shift to plant-based precursors in the mid-20th century, pioneered by processes involving diosgenin from Mexican yams, addressed limitations in supply scalability and ethical concerns over animal sourcing, drastically lowering production costs from over $200 per gram of cortisone in 1948 to pennies per gram by the 1950s.^[139]^[136] Precursor selection prioritizes materials with high global abundance, such as yam-derived diosgenin (with global production of approximately 3,000 to 6,000 tons annually) and soybean phytosterols, to ensure cost-effectiveness below $10 per kilogram for bulk industrial use. Structural similarity to steroids minimizes synthetic steps; for instance, diosgenin's intact tetracyclic core closely mirrors the gonane skeleton of target hormones, reducing transformation complexity compared to acyclic petrochemical alternatives.^[135]^[137]^[142]

Semisynthesis Approaches

Semisynthesis of steroids involves the partial chemical or biocatalytic modification of naturally occurring steroid precursors to produce pharmaceutically relevant compounds, leveraging the core tetracyclic structure of these molecules while introducing specific functional groups. This approach contrasts with total synthesis by relying on abundant plant-derived scaffolds, such as sapogenins, to achieve economically viable production scales. Key methods include degradative processes to remove side chains and selective functionalizations via oxidation, reduction, or microbial hydroxylations, often yielding intermediates like pregnenolone derivatives that serve as building blocks for hormones and corticosteroids.^[143] One foundational semisynthetic technique is the Marker degradation, developed in the late 1930s, which removes the spiroketal side chain from diosgenin—a steroidal sapogenin extracted from yams—to yield 16-dehydropregnenolone acetate (16-DPA), a versatile precursor for progesterone and other steroids. The process typically involves acetylation of the diosgenin, followed by acid-catalyzed degradation and hydrolysis under controlled conditions, such as using acetic acid in the final step to optimize the acetate ester formation. This multi-step degradation achieves overall yields of more than 60% from diosgenin, enabling large-scale production that revolutionized steroid availability during the 1940s.^[144] Microbial transformations represent another cornerstone of steroid semisynthesis, particularly for introducing hydroxyl groups at specific positions that are challenging to achieve chemically. A seminal example is the 11α-hydroxylation of progesterone using fungi from the genus Rhizopus, such as Rhizopus nigricans or Rhizopus oryzae, which selectively hydroxylate the C11 position to produce 11α-hydroxyprogesterone, an intermediate en route to hydrocortisone. Discovered in 1950 by the Upjohn team, this biotransformation exploits cytochrome P450 enzymes in the fungal cells, proceeding under mild aqueous conditions with conversions often exceeding 80% and high regioselectivity. Subsequent chemical steps, such as oxidation of the 11α-hydroxyl to a ketone and 21-hydroxylation, complete the synthesis of hydrocortisone.^[145]^[146] Chemical modifications complement these degradative and biocatalytic steps, including oxidations (e.g., using chromium reagents to form ketones at C3 or C20), reductions (e.g., sodium borohydride for alcohol formation), and protection strategies like acetylation of hydroxyl groups to prevent side reactions. These operations are typically performed in sequence on Marker-derived intermediates, with individual steps yielding 70-90% to maintain overall process efficiency. For instance, acetylation not only protects but also facilitates purification, as seen in the conversion of 16-DPA to downstream pregnane derivatives. A notable historical milestone in semisynthesis is the 1950s Upjohn process for producing corticosteroids from stigmasterol, a phytosterol abundant in soybean oil. This method involved side-chain cleavage via ozonolysis or periodate oxidation to generate progesterone, followed by the aforementioned Rhizopus-mediated 11α-hydroxylation and chemical adjustments to yield compounds like hydrocortisone and prednisone. By integrating microbial and chemical steps, Upjohn achieved commercial scalability, reducing reliance on scarce animal sources and enabling the mass production of anti-inflammatory drugs during the post-war era.^[141]^[147] The advantages of semisynthetic approaches lie in their cost-effectiveness for pharmaceutical production, as they utilize inexpensive plant precursors and achieve high stereoselectivity through biocatalysis, often at lower energy costs than total synthesis. Recent advancements in the 2020s have focused on engineered enzymatic semisynthesis, including chemoenzymatic cascades with cytochrome P450 variants for site-selective C14-functionalization of androstane scaffolds, yielding diverse hydroxylated steroids with 72-95% conversion and up to 90% selectivity. These methods, such as whole-cell biotransformations with optimized P450 enzymes, enhance sustainability by minimizing organic solvents and enabling modular assembly of bioactive analogs.^[148]

Total Synthesis Methods

Total synthesis of steroids involves the complete laboratory construction of the tetracyclic steroidal skeleton and its functional groups starting from simple, non-steroidal precursors such as ketones or acyclic compounds, distinguishing it from semisynthetic modifications of natural steroids.^[149] This approach has been pivotal for accessing rare natural products and designing novel analogs, though it demands precise control over multiple carbon-carbon bond formations and stereogenic centers.^[150] A cornerstone method in early steroid total synthesis is the Robinson annulation, developed by Robert Robinson in 1935, which facilitates the construction of the A/B ring system through a Michael addition followed by aldol condensation of a ketone, typically 2-methylcyclohexanone, with methyl vinyl ketone to form a fused cyclohexenone.^[151] This reaction efficiently builds the six-membered rings essential to the steroid core and has been widely employed in subsequent syntheses for its ability to introduce unsaturation and stereochemistry at key junctions.^[149] One seminal achievement is Robert B. Woodward's 1951 total synthesis of cortisone, accomplished in 36 steps from inexpensive precursors like norbornadiene and achieving the first complete chemical replication of a therapeutically important steroid hormone.^[152] This landmark work demonstrated the feasibility of assembling the full steroidal framework, including side-chain elaboration, and inspired asymmetric variants using chiral auxiliaries to control stereochemistry at the eight chiral centers.^[150] Major challenges in steroid total synthesis include achieving stereocontrol at quaternary carbon centers and ring fusions, as well as managing multiple redox transformations, which historically resulted in overall yields below 1% due to epimerization and side reactions.^[150] Advances in catalysis have improved efficiencies, with modern routes attaining 10-20% overall yields through selective bond-forming steps.^[149] In the 2010s, palladium-catalyzed couplings emerged as transformative for side-chain installation and ring closure; for instance, an enantioselective intramolecular Heck reaction constructed the boldenone core in 90% yield with >99:1 diastereoselectivity.^[150] More recent organocatalytic methods, post-2020, have enabled asymmetric cascades for steroid skeletons, such as chiral Brønsted acid-catalyzed syntheses of estrone derivatives with 93% ee, offering milder conditions and higher selectivity for complex natural products.^[149] These total syntheses find primary applications in producing rare natural steroids unavailable from biological sources and in generating structural analogs for pharmacological research, such as modified cardiotonic steroids.^[150]

Historical Research and Recognition

Key Discoveries and Milestones

In the 1930s, significant advances in steroid isolation laid the foundation for understanding their hormonal roles. Adolf Butenandt and colleagues successfully isolated crystalline progesterone from sow ovaries in 1934, marking the first purification of this key progestogen essential for pregnancy maintenance.^[153] Concurrently, Heinrich Otto Wieland determined the correct molecular structure of cholesterol by 1932, confirming its tetracyclic sterol framework and linking it to bile acids, which advanced knowledge of lipid-derived hormones.^[154] The 1940s and 1950s saw breakthroughs in therapeutic applications, particularly with adrenal corticosteroids. In 1948, Merck & Co. achieved the first large-scale chemical synthesis of cortisone through a 37-step process starting from bile acids, enabling clinical trials that demonstrated its dramatic efficacy in alleviating rheumatoid arthritis symptoms by suppressing inflammation.^[155] This synthesis, combined with Philip Hench's administration of cortisone to patients, revolutionized treatment for autoimmune and inflammatory disorders, shifting steroids from biochemical curiosities to vital pharmaceuticals.^[156] By the 1960s, research shifted toward molecular mechanisms of steroid action. Elwood V. Jensen identified the estrogen receptor in 1958 using tritium-labeled estradiol, revealing that estrogens bind to specific intracellular proteins in target tissues like the uterus, which undergo conformational changes to regulate gene expression.^[157] This discovery established the paradigm of steroid hormone receptors as nuclear transcription factors, influencing subsequent studies on endocrine signaling and breast cancer therapies. The 1970s brought the recognition of steroids beyond animals, with the discovery of brassinosteroids in plants. In 1970, James A. D. Mitchell and coworkers isolated brassinolide from rapeseed pollen, identifying it as a growth-promoting sterol that enhances stem elongation and stress resistance. Signaling pathways for brassinosteroids were further elucidated in the 1980s through physiological assays, showing their role in cell expansion via receptor-mediated transduction, expanding the scope of steroid functions to plant development.^[158] In the 2010s, gene-editing technologies enabled precise interrogation of steroid biosynthesis. A 2018 CRISPR/Cas9 study targeting the Tspo gene in MA-10 mouse tumor Leydig cells suggested a role for TSPO in cholesterol transport by showing reduced mitochondrial function and steroid production in mutants, though the essentiality of TSPO in steroidogenesis remains controversial based on in vivo models.^[159] These investigations highlighted genetic regulation in steroid pathways, paving the way for therapeutic interventions. In 2024, BridgeBio Pharma reported topline results from a Phase 1/2 trial of BBP-631, an AAV-based gene therapy targeting the CYP21A2 gene for congenital adrenal hyperplasia (CAH), which demonstrated increased endogenous cortisol production and reduced androgen excess at higher doses in patients. However, in September 2024, BridgeBio discontinued further development of the therapy, citing that the results did not meet expectations for transformational impact, and sought partnership opportunities.^[160]^[161]

Notable Awards and Contributors

Several Nobel Prizes have recognized groundbreaking contributions to steroid research. In 1927, Heinrich Otto Wieland received the Nobel Prize in Chemistry for elucidating the structure of bile acids, which are steroidal compounds essential to understanding cholesterol metabolism. The 1939 Nobel Prize in Chemistry was awarded jointly to Adolf Butenandt and Leopold Ruzicka for their pioneering isolation and synthesis of male and female sex hormones, including androsterone and estrone, advancing knowledge of steroid endocrinology. In 1950, Edward C. Kendall, Philip S. Hench, and Tadeus Reichstein shared the Nobel Prize in Physiology or Medicine for discoveries concerning the hormones of the adrenal cortex, their structure, and biological effects, including the isolation of cortisone, which revolutionized treatment for inflammatory diseases. Additionally, the 1985 Nobel Prize in Physiology or Medicine went to Michael S. Brown and Joseph L. Goldstein for their work on the regulation of cholesterol metabolism, identifying LDL receptors and pathways that control endogenous steroid precursor synthesis, influencing statin development. Other prestigious awards have honored steroid-related innovations in endocrinology and pharmacology. The 1960 Albert Lasker Award for Basic Medical Research was presented to Gregory Pincus for developing the first oral contraceptive pill based on synthetic progestins, transforming reproductive health. In 1989, Étienne-Émile Baulieu received the Albert Lasker Clinical Medical Research Award for discovering mifepristone (RU486), a synthetic steroid antagonist that enables medical abortion.^[162] The 2004 Albert Lasker Award for Basic Medical Research recognized Elwood V. Jensen, Bert W. O'Malley, and Ronald M. Evans for elucidating nuclear receptors that mediate steroid hormone actions, such as estrogen and glucocorticoid signaling.^[163] In the agricultural sciences, the 2024 Wolf Prize in Agriculture was awarded to Joanne Chory for mapping plant steroid hormone signaling pathways, enhancing crop resilience. Key contributors have shaped steroid science through seminal isolations, syntheses, and clinical applications. Tadeus Reichstein isolated over 60 distinct steroids from adrenal extracts, providing the foundation for hormone therapeutics. Adolf Butenandt advanced sex hormone biochemistry by characterizing their chemical structures and biosynthetic pathways. Leopold Ruzicka contributed total syntheses of complex steroids like testosterone, enabling scalable production. Rachmiel Levine explored adrenal steroids' roles in carbohydrate metabolism, demonstrating cortisone's effects on glucose transport and insulin action in diabetes models.^[164] Michael S. Brown's research on cholesterol homeostasis revealed feedback mechanisms inhibiting HMG-CoA reductase, the target of statins that modulate steroid precursor levels. While historical recognition has often centered on male scientists, contributions from women in steroid-related fields remain underrepresented, alongside recent advances in phytosterol research by diverse teams.

References

[1]
Steroids | C26H44N2O | CID 139082353 - PubChem - NIH
Steroid Compound is compounds possessing the skeleton of cyclopenta[a]phenanthrene or a skeleton derived therefrom by one or more bond scissions or ring ...
[2]
MeSH - Steroids - NCBI - NIH
Steroids. A group of polycyclic compounds closely related biochemically to TERPENES. They include cholesterol, numerous hormones, precursors of certain ...
[3]
Understanding the role of steroids in typical and atypical brain ...
Steroid hormones are cyclical chemical compounds made up of rings of carbon atoms that play an essential role in a wide range of physiological functions ...
[4]
Reproductive Steroid Hormones: Synthesis, Structure, and ... - NCBI
Steroid hormones can be classified into four groups: (1) natural hormones found in nature and formulated into drugs without undergoing chemical modifications; ...
[5]
Physiology, Glucocorticoids - StatPearls - NCBI Bookshelf
Jul 17, 2023 · Glucocorticoids are steroid hormones produced from the cortex of adrenal glands (gluco-corti-coids: glucose-cortex-steroids).<|control11|><|separator|>
[6]
The science of steroids - PubMed
May 23, 2019 · Steroids are complex lipophilic molecules that have many actions in the body to regulate cellular, tissue and organ functions across the life-span.Missing: chemistry | Show results with:chemistry
[7]
Steroids: Pharmacology, Complications, and Practice Delivery Issues
This review summarizes the basic pharmacology, complications, and practice delivery issues regarding steroids.
[8]
Prednisone and other corticosteroids: Balance the risks and benefits
Corticosteroid medicines are used to treat rheumatoid arthritis, inflammatory bowel disease (IBD), asthma, allergies and many other conditions. They also are ...
[9]
Anabolic Steroids - StatPearls - NCBI Bookshelf - NIH
Feb 6, 2025 · These agents play a critical role in managing conditions such as primary hypogonadism, delayed male puberty, hypogonadotropic and idiopathic ...<|control11|><|separator|>
[10]
The History of Cortisone Discovery and Development - PubMed
This article examines the paths that led to the day when the first rheumatoid arthritis patient received cortisone, and from there to the 1950 Nobel Prize ...
[11]
The interaction of steroids with phospholipid bilayers and membranes
Dec 2, 2021 · Most biophysical experiments of steroid-membrane interactions are carried out at steroid concentrations ranging from 2 to 25 mol%, sometimes ...
[12]
Free Diffusion of Steroid Hormones Across Biomembranes
According to the genomic mechanism, the lipophilic character of the hormones enables them to dissociate spontaneously from the carriers and enter the target ...<|control11|><|separator|>
[13]
27.6 Steroids – Organic Chemistry: A Tenth Edition
Substituent groups on the steroid ring system can be either axial or equatorial. As with simple cyclohexanes, equatorial substitution is generally more ...
[14]
26.7: Steroids - Chemistry LibreTexts
May 30, 2020 · The generic steroid structure drawn above has seven chiral stereocenters (carbons 5, 8, 9, 10, 13, 14 & 17), which means that it may have as ...
[15]
Biological Activity and Structural Diversity of Steroids Containing ...
This review provides an overview of the biological activities of steroids and isoprenoid lipids derived from diverse natural sources.<|control11|><|separator|>
[16]
[PDF] NOMENCLATURE OF STEROIDS - iupac
These recommendations are numbered 3s-1, 3s-2, 3s-3, etc., the initial 3 denoting that this is the third revised set of recommendations. 3s- 1. GENERAL. A 3s- ...
[17]
[PDF] DEFINITIVE RULES FOR NOMENCLATURE OF STEROIDS - iupac
These Rules are issued by the IUPAC Commission on the Nomenclature of Organic Chemistry and by the IUPAC—IUB Commission on Biochemical Nomenclature. Those who ...
[18]
Steroid hormones: relevance and measurement in the clinical ...
The steroid hormones are synthesized in the adrenal cortex, the gonads, and the placenta; are all derived from cholesterol and many are of clinical importance.
[19]
Review: microbial transformations of human bile acids - Microbiome
Jun 14, 2021 · BAs play an important role in regulating various physiological systems, such as fat digestion, cholesterol metabolism, vitamin absorption ...
[20]
The role of bile acids in metabolic regulation in
Bile acids (BA), long believed to only have lipid-digestive functions, have emerged as novel metabolic modulators. They have important endocrine effects ...
[21]
The Multifunctional Fungal Ergosterol - PMC - PubMed Central - NIH
Sep 18, 2018 · Ergosterol, a 5,7-diene oxysterol, is the most abundant sterol in fungal cell membranes, where it regulates permeability and fluidity (1).
[22]
Advances in the Biosynthesis and Molecular Evolution of Steroidal ...
In plants, steroidal saponins, like other plant antitoxins, act as a line of defense against infection by pathogens. The difference is that steroidal saponins ...
[23]
Saponins, the Unexplored Secondary Metabolites in Plant Defense
Mar 10, 2025 · Based on the aglycone structure, they are classified into three types: steroidal saponins, terpenoid saponins and steroidal glycoalkaloids.
[24]
[PDF] Steroids: A Diverse Class of Secondary Metabolites - Hilaris Publisher
Jul 15, 2015 · The cardiac glycosides may be classified as cardenolide and /or bufadienolides ... The bile acids are the steroid acids found predominantly in the.
[25]
Steroids as Environmental Compounds Recalcitrant to Degradation
Steroids are tetracyclic triterpenoid lipids containing a perhydro-1,2-cyclopentanophenanthrene structure, and include sterols, bile acids, steroid hormones, ...
[26]
Chemistry and the Potential Antiviral, Anticancer, and Anti ...
Cardiotonic steroids are divided into two distinct groups: cardenolides (plant-derived) and bufadienolides (mainly of animal origin). Cardenolides have an ...Missing: secosteroids | Show results with:secosteroids
[27]
Naturally Occurring Norsteroids and Their Design and ...
Both B-norsteroids and abeo-steroids are based on the core steroid structure, sharing the basic four-ring configuration common to all steroids.
[28]
Classic and current concepts in adrenal steroidogenesis: a reappraisal
The term pregnane is assigned to cholesterol derivatives whose molecules contain 21 carbon atoms (C21); and androstane (C19) and estrane (C18) to those with 19 ...
[29]
Steroid Hormone - an overview | ScienceDirect Topics
Pregnenolone and its derivatives that contain 21 carbon atoms are often termed C21 steroids. ... C19 androgens to C18 estrogens. With the exception of estrogens, ...Missing: classification | Show results with:classification
[30]
Vitamin D: Production, Metabolism, and Mechanism of Action - NCBI
Jun 15, 2025 · Vitamin D3 is produced in the skin from 7-dehydrocholesterol by UV irradiation, which breaks the B ring to form pre-D3. Pre-D3 isomerizes to D3 ...
[31]
Brassinosteroids - PMC - PubMed Central - NIH
The structure of brassinolide is presented with possible variations in the A and B rings and the side chain shown in boxes. Compounds to the right have been ...
[32]
Effects of steroid D-ring modification on suicide inactivation and ...
Effects of steroid D-ring modification on suicide inactivation and competitive inhibition of aromatase by analogs of androsta-1,4-diene-3,17-dione. Click to ...Missing: implications | Show results with:implications
[33]
Influence of cholesterol and ergosterol on membrane dynamics
Sterols are essential membrane components of eukaryotic cells and are important for membrane organization and function. Cholesterol is the most ...
[34]
Sterols and membrane dynamics - PMC - PubMed Central - NIH
It is shown that all sterols share a common property: the ability to regulate dynamics in order to maintain membranes in a microfluid state.Sterols And Membrane... · Sterols And The ``raft''... · Sterols In Complex ``live''...
[35]
Functional convergence of hopanoids and sterols in membrane ...
Prokaryotes generally lack sterols; however, some bacteria produce hopanoids (5, 6), which are structurally similar (Fig. 1A) (7), and their cyclization is ...
[36]
Phylogenomics of Sterol Synthesis: Insights into the Origin ... - NIH
Sterol biosynthesis and prokaryotes-to-eukaryotes evolution. Biochem Biophys ... A novel gene conserved from yeast to humans is involved in sterol biosynthesis.
[37]
Evolution of the Cholesterol Biosynthesis Pathway in Animals
Aug 9, 2019 · CBP genes in the basal metazoans were inherited from their last common eukaryotic ancestor and evolutionarily conserved for cholesterol ...
[38]
Phytosterols | Linus Pauling Institute | Oregon State University
Plant foods providing phytosterols into the modern diet could improve serum lipid (cholesterol) profiles and help reduce the risk of cardiovascular disease.
[39]
Stigmasterol: An Enigmatic Plant Stress Sterol with Versatile Functions
Jul 25, 2024 · Thus, sterols play important diverse functions in plants [6]. Stigmasterol is an unsaturated sterol that is the end product of the phytosterol ...
[40]
Sterol-Rich Plasma Membrane Domains in Fungi | Eukaryotic Cell
For example, on a molar basis, the yeast plasma membrane lipid content is similar to that of mammalian cells in that it is about 30 to 40% sterols, 10 to 20% ...Missing: percentage | Show results with:percentage<|control11|><|separator|>
[41]
Role of Cholesterol in the Formation and Nature of Lipid Rafts ... - NIH
Cholesterol has been connected to the possible formation of ordered lipid domains (rafts) in mammalian cell membranes.
[42]
Cholesterol, lipid rafts, and disease - PMC - PubMed Central - NIH
Cholesterol is thought to serve as a spacer between the hydrocarbon chains of the sphingolipids and to function as a dynamic glue that keeps the raft assembly ...
[43]
Hopanoid lipids: from membranes to plant–bacteria interactions - PMC
Feb 19, 2018 · Hopanoids, which resemble sterols and are found in the membranes of diverse bacteria, have left an extensive molecular fossil record.
[44]
Terpenoids and membrane dynamics evolution - Frontiers
While hopanoids are formed by direct cyclization of squalene, steroids are formed by cyclization of 'oxygenated' squalene (oxidosqualene). Accordingly, steroid ...<|control11|><|separator|>
[45]
Novel sterol binding domains in bacteria - PMC - PubMed Central
The aerobic methanotroph Methylococcus capsulatus was the first bacterium shown to synthesize sterols, producing a mixture of C-4 methylated sterols that are ...
[46]
De novo cholesterol biosynthesis in bacteria | Nature Communications
May 22, 2023 · Here, we show that the marine myxobacterium Enhygromyxa salina produces cholesterol and provide evidence for further downstream modifications.
[47]
Evolution of bacterial steroid biosynthesis and its impact on ... - NIH
Jun 15, 2021 · Our results indicate that steroid biosynthesis evolved within bacteria in response to the rise of oxygen and was later horizontally transferred to eukaryotes.
[48]
Steroidomics via Gas Chromatography–Mass Spectrometry (GC-MS)
GC-MS remains a cornerstone and often considered a reference method for comprehensive steroid profiling, particularly for isomeric compounds and low-abundance ...Missing: prokaryotes | Show results with:prokaryotes
[49]
Recent Advances in Ergosterol Biosynthesis and Regulation ... - NIH
In the pharmaceutical industry, ergosterol is a precursor of vitamin D2 and steroid hormone drugs [5]. ... Ergosterol is the main sterol of fungi and it carries ...
[50]
Enrichment of vitamin D2 in mycelium from submerged cultures of ...
Ergosterol, a precursor of vitamin D2, is present in the cell membrane of all fungi. It can be transformed into vitamin D2 by UV-B exposure.
[51]
Phylogenetic distribution of fungal sterols - PubMed
May 28, 2010 · ... brassicasterol), and intermediates in the formation of 24-ethyl cholesterol, are major sterols in 175 species of Fungi. Although most fungi ...
[52]
Distribution of sterols in fungi. II. Brassicasterol in Tuber and Terfezia ...
Terfezia sp., on the other hand, contained about 98% brassicasterol with only small amounts of ergosterol. Brassicasterol is common to several families of the ...
[53]
β-Sitosterol differentially regulates key metabolites for growth ...
May 29, 2021 · This current study aims to investigate the effects of exogenous application of β-sitosterol (βSito) on growth improvement and tolerance level of rice plants ...
[54]
Brassinosteroid Regulates Cell Elongation by Modulating ...
Among the plant hormones, brassinosteroid (BR) and gibberellin (GA) are the two most important ones that determine plant height by regulating cell elongation.
[55]
Ecdysteroidogenesis - an overview | ScienceDirect Topics
The precursors for ecdysteroid synthesis by the prothoracic gland of insects are sterols, such as cholesterol (Fig. 1.29). Sterols are also necessary components ...
[56]
Practical uses for ecdysteroids in mammals including humans
20E (5 mg/kg per os) stimulates the conversion of cholesterol into bile acids in rats (Syrov et al., 1986b), and such an effect is reminiscent of some ...
[57]
Sterol metabolism in the filasterean Capsaspora owczarzaki ... - NIH
Jul 6, 2016 · Cholesterol predominates in derived bilaterians such as vertebrates, tunicates, annelids, arthropods and molluscs, although other sterols might ...
[58]
Diversity of Plant Sterols Metabolism: The Impact on Human Health ...
May 12, 2021 · For example, the content of naturally occurring sterols in fruits and vegetables is ranged between 38 to 439 mg/kg of fresh weight, whereas in ...
[59]
Control of Cholesterol Turnover in the Mouse - ScienceDirect.com
Typically, the mouse synthesizes ∼160 mg/day/kg of cholesterol whereas the human makes only ∼10 mg/day/kg (column B). As in other rodents, the liver of the ...
[60]
Exploiting microalgal diversity for sterol production - PMC - NIH
Jun 30, 2025 · The extensive variability in microalgal sterol profiles reveals a sophisticated interplay between genetic inheritance, ecological adaptation, ...
[61]
An investigation of the mechanisms for sterol synthesis and dietary ...
In freshwater ecosystems sterol production and modification of dietary sterols has been most closely associated with essential lipid upgrading (Bec et al., ...
[62]
Cholesterol in the Cell Membrane—An Emerging Player in ...
Jan 4, 2022 · It affects the physiological features of the cell membrane by controlling its fluidity, and regulating the negative membrane curvature through ...
[63]
Nuclear receptors in health and disease: signaling pathways ...
Jul 28, 2025 · Nuclear receptors (NRs) are a class of ligand-activated transcription factors that sense certain molecules, such as steroids, thyroid hormones ...
[64]
Impact of steroid biosynthesis on the aerobic adaptation of eukaryotes
Jul 5, 2024 · Steroids are critical membrane regulators and signaling agents in eukaryotes that are evolutionarily related to hopanoids—the structural and ...
[65]
Low-density lipoproteins cause atherosclerotic cardiovascular ...
Regardless of the underlying genetic defect, FH is characterized by markedly elevated levels of LDL-C and premature atherosclerosis, particularly coronary ...Missing: deficiencies hypocholesterolemia
[66]
Ecological Significance of Sterols in Aquatic Food Webs
Sterols control membrane fluidity and permeability in all eukaryotic cells, and the ordering capacity provided by cholesterol is different to that provided by ...<|control11|><|separator|>
[67]
Effect of increase in cortisol level due to stress in healthy young ...
In our study, cortisol levels in stressed period were found to increase about 9 times compared with relaxed periods. This result was expected and our values ...
[68]
Functions of Steroid Hormones in the Male Reproductive Tract as ...
Feb 1, 2023 · Three classes of steroid hormones, namely androgens, estrogens and glucocorticoids, contribute to the development of the reproductive system ...
[69]
Control of the insect metamorphic transition by ecdysteroid ...
Sep 17, 2020 · Ecdysteroids are a class of steroid hormones that controls molting and metamorphic transitions in Ecdysozoan species including insects.Missing: arthropods | Show results with:arthropods
[70]
The Control of Cell Expansion, Cell Division, and Vascular ...
Mar 4, 2020 · Here we review the roles of brassinosteroids in cell expansion, cell division, and vascular differentiation, comparing the early physiological studies with ...<|separator|>
[71]
Brassinosteroids: Multidimensional Regulators of Plant Growth ...
The plant steroid hormone brassinosteroids (BRs) play important roles in plant growth and development, regulating diverse processes such as cell elongation, ...
[72]
Phytosterols Play a Key Role in Plant Innate Immunity against ... - NIH
Our results show that sterol content plays an important role in plant innate immunity against bacterial infections by regulating nutrient efflux into the ...
[73]
Azoles activate type I and type II programmed cell death pathways in ...
May 31, 2024 · Antifungal azoles inhibit ergosterol biosynthesis, but how that leads to fungistatic or fungicidal activities in many pathogenic fungi is poorly ...
[74]
Plant Sterols and Plant Stanols in Cholesterol Management ... - NIH
The main putative mechanism of cholesterol reduction through the consumption of plant sterols/stanols is the inhibition of cholesterol absorption from the ...
[75]
ENZYMES OF THE MEVALONATE PATHWAY OF ISOPRENOID ...
The mevalonate pathway accounts for conversion of acetyl-CoA to isopentenyl 5-diphosphate, the versatile precursor of polyisoprenoid metabolites and natural ...
[76]
Physiology and Pathophysiology of Steroid Biosynthesis, Transport ...
Sep 12, 2018 · It participates in biosynthesis and metabolism of steroids as well as their regulated exchange between maternal and fetal compartment.Missing: trans | Show results with:trans<|control11|><|separator|>
[77]
Production of Squalene in Bacillus subtilis by Squalene Synthase ...
Squalene synthase (SQS) catalyzes the conversion of two farnesyl pyrophosphates to squalene, an important intermediate in between isoprene and valuable ...
[78]
Production of Squalene in Bacillus subtilis by Squalene Synthase ...
Squalene synthase (SQS) catalyzes the conversion of two farnesyl pyrophosphates to squalene, an important intermediate in between isoprene and valuable ...
[79]
An Overview of the Cholesterol Biosynthesis Pathway
Feb 3, 2020 · The first step of the next part of the cholesterol biosynthesis pathway is the utilization of the squalene epoxidase enzyme to catalyze the ...Missing: review | Show results with:review
[80]
Biosynthesis of Cholesterol and Other Sterols | Chemical Reviews
Sep 8, 2011 · The stoichiometry of cholesterol synthesis from lanosterol is therefore lanosterol + 15NADPH + 4H+ + 10O2 → cholesterol + 2CO2 + HCOOH + 15NADP+ ...
[81]
Cholesterol Biosynthesis from Lanosterol: MOLECULAR CLONING ...
The 19-step conversion of lanosterol to cholesterol in mammals begins with C14-demethylation and is followed by C14-reduction, C4-demethylation, C8→7 ...
[82]
Novel activities of CYP11A1 and their potential physiological ... - NIH
CYP11A1, found only in vertebrates, catalyzes the first step of steroidogenesis where cholesterol is converted to pregnenolone.
[83]
The neurosteroid pregnenolone is synthesized by a mitochondrial ...
In peripheral steroidogenic tissues, cholesterol is converted to the major steroid precursor pregnenolone by the CYP11A1 enzyme. Although pregnenolone is one of ...
[84]
Methylerythritol Phosphate Pathway of Isoprenoid Biosynthesis - NIH
Sep 21, 2016 · The MEP pathway has been identified in eubacteria, green algae, and higher plants, whereas the MVA pathway is found in animals, plants (cytosol) ...
[85]
Isoprenoid biosynthesis: The evolution of two ancient and ... - PNAS
Two distinct routes of IPP biosynthesis occur in nature: the mevalonate pathway and the recently discovered deoxyxylulose 5-phosphate (DXP) pathway.Missing: predates | Show results with:predates
[86]
Isoprenoid biosynthesis in plant chloroplasts via the MEP pathway
Two biosynthetic pathways for isoprenoid biosynthesis are present in plants. The mevalonate pathway is found in the cytoplasm, whereas the MEP pathway is only ...
[87]
Cross-talk Between the Cytosolic Mevalonate and the Plastidial ...
Incorporation studies with [1,1,1,4-2H4]DX showed that sterols, normally derived from MVA, in the presence of mevinolin are synthesized via the MEP pathway.
[88]
Cross-talk between the Cytosolic Mevalonate and the Plastidial ...
In plants, two pathways are utilized for the synthesis of isopentenyl diphosphate, the universal precursor for isoprenoid biosynthesis.
[89]
Quantitative assessment of crosstalk between the two isoprenoid ...
Plants have been shown to use the mevalonate pathway for the biosynthesis of sterols and triterpenes in the cytoplasm and the recently discovered deoxyxylulose ...
[90]
Biosynthesis of lanosterol in Escherichia coli - ScienceDirect.com
In this study, we have successfully reconstituted the biosynthesis of the eukaryotic lipid lanosterol in E. coli. The successful synthesis of lanosterol ...
[91]
Sterol Synthesis in Diverse Bacteria - Frontiers
Sterols are essential components of eukaryotic cells whose biosynthesis and function has been studied extensively. Sterols are also recognized as the ...
[92]
On the Origin of Isoprenoid Biosynthesis - PMC - NIH
Jun 14, 2018 · Photosynthetic eukaryotes are known to have both the MVA and the MEP pathways, and the MEP pathway is inferred to have originated from an ...Missing: predates prokaryotes
[93]
regulating artemisinin biosynthesis in Artemisia annua L - PMC - NIH
These 5-carbon building blocks of all terpenes can originate from either the cytosolic mevalonate (MVA) pathway or the methylerythritol phosphate (MEP) pathway ...
[94]
Human steroid biosynthesis, metabolism and excretion are ...
We briefly review the origins of steroid hormones, and present the resulting serum metabolome of each of the main classes of steroids.<|control11|><|separator|>
[95]
Bile Acid Biology, Pathophysiology, and Therapeutics - PMC
Apr 4, 2020 · Bile acids are synthesized from cholesterol exclusively in the liver and function as physiological detergents that facilitate biliary cholesterol excretion.
[96]
Bile acid metabolism and signaling in cholestasis, inflammation and ...
In cholestasis, impaired bile flow leads to accumulation of bile acids in the liver, causing hepatocyte and biliary injury and inflammation.
[97]
Intratumoral Steroid Profiling of Adrenal Cortisol-Producing ...
Jun 2, 2021 · Steroid extraction and quantification by LC-MS/MS. Steroids were extracted by the Folch extraction method as previously described.
[98]
A comprehensive method for extraction and quantitative analysis of ...
Traditionally, the extraction of sterols has relied on one of two classic methods: Bligh/Dyer method or Folch method (16, 17). Both make use of a mixture of ...
[99]
β-Sitosterol: Supercritical Carbon Dioxide Extraction from Sea ...
Apr 22, 2010 · Supercritical fluid extraction represents an efficient and environmentally friendly technique for isolation of phytosterols from different ...
[100]
Phytosterols and their extraction from various plant matrices using ...
Jul 22, 2014 · Several studies have been performed to extract phytosterols using supercritical CO 2 (SC-CO 2 ) and this technology has clearly offered potential advantages ...INTRODUCTION · SC-CO2 EXTRACTION OF... · EXPERIMENTAL DESIGNS...Missing: CO2 | Show results with:CO2
[101]
Thin-layer separation and quantification of bile acids - PubMed
Bile acids are determined directly in serial areas of silica gel by treating gel areas suspended in tris buffer with resazurin reagent. The method is ...
[102]
Separation of Selected Bile Acids by TLC. VIII. Separation on Silica ...
In the present study, to separate a/m bile acids using adsorption thin layer chromatography at 18°C, the glass plates precoated with silica gel 60F254 (#1.05715) ...
[103]
Feasibility study of separation and purification of bile acid ...
In this research, a group of bile acid derivatives were subjected to HPLC analysis using four experimental systems, which presented combinations of C18 and F5 ...
[104]
Factors affecting separation and detection of bile acids by liquid ...
This work developed three chromatographic gradient methods to separate a total of 48 BA standards on BEH C18 column and HSS T3 column and accordingly unraveled ...
[105]
The Isolation of Thyroxine and Cortisone: the Work of Edward C ...
May 24, 2002 · Edward C. Kendall isolated thyroxine from the thyroid gland, he crystallized glutathione and determined its structure, and he isolated and ...
[106]
[PDF] Edward C. Kendall - Nobel Lecture
The first investigations which contributed to the development of cortisone came in 1929 from physiologists. Two groups of workers, Hartman and his.
[107]
History of the development of corticosteroid therapy - PubMed
Oct 21, 2011 · The first clinical evidence that an extract of animal adrenocortical tissue could counteract human adrenal failure was demonstrated in 1930.
[108]
Comparative study of different methods of isolation of cholesterol.
Wool grease or lanolin contains upto 15 percent cholesterol in the form of its ester. This cholesterol can be isolated from the wool grease by several meth ...Missing: extraction | Show results with:extraction
[109]
Cholesterol | C27H46O | CID 5997 - PubChem - NIH
Cholesterol is commercially prepared from bovine spinal cord and wool grease by extraction with petroleum ether. The extracted material is then purified by ...
[110]
Preparation of cholesterol from wool grease by means of addition ...
After separating the addition product, it is decomposed by water, yielding the cholesterol, which is purified by crystallization. References.
[111]
Infrared Spectrometry Applied to Steroid Structure and Metabolism
These permit the presence of all the functional groups which commonly occur in steroids to be recognized. In the case of carbonyl groups and double bonds ...
[112]
[PDF] Infrared Spectra of Steroids - Russian Chemical Reviews
This review covers the infrared spectra of steroids, including their chemical structure, correlation with spectra, and use in chemistry, from 1939 to 1966.
[113]
Infrared Absorption Spectra of Steroids. An Atlas. - Science
Most of the spectra are of steroid hormones and of their metabolites and deriva- tives, but representative curves for steroid alcohols, bile acid esters, ...
[114]
Nuclear Magnetic Resonance of Steroids
Dec 14, 2016 · In the following chapter, the nuclear magnetic resonance (NMR) spectroscopic investigation of steroids is described.An Introduction to Steroids · Recent General NMR... · Covariance NMR and SteroidsMissing: seminal | Show results with:seminal
[115]
Complete 1 H NMR Assignment of Diosgenin Benzoate
Jul 20, 2023 · The 750-MHz 1 H NMR spectrum of diosgenin benzoate (1b) has been completely assigned by means of QM-based 1 H iterative full spin analysis (HiFSA).Introduction · Results And Discussion · Interpretation Of The H Nmr...
[116]
A Systematic NMR Approach for the Determination of the Molecular ...
A Systematic NMR Approach for the Determination of the Molecular Structure of Steroidal Saponins. Chapter. pp 299–315; Cite this chapter. Download book PDF.Missing: seminal papers
[117]
Some aspects of mass spectrometry in research on steroids - Journals
Detailed studies based on pure steroids and their isotope labelled analogues have established the major pathways of fragmentation under electron impact. The ...
[118]
[PDF] Mass spectral fragmentation analyses of isotopically labelled ...
Feb 26, 2020 · Mass spectrometry in the analysis of steroid drugs and their metabolites: Electron-impact- induced fragmentation of ring D. J Pharm ...<|separator|>
[119]
Low‐energy electron ionization optimization for steroidomics ...
Sep 9, 2021 · We present a multifactorial study on optimizing a low-energy electron ionization source to maximize molecular ion formation while minimizing the extent of ...
[120]
Dorothy Hodgkin: Cracking crystal codes | Feature - Chemistry World
Jun 25, 2010 · With her graduate student Harry Carlisle, she finally confirmed the 3D molecular structure of cholesterol iodide by x-ray analysis in 1942.
[121]
X-Ray crystallography and the chemistry of the steroids. Part I
A survey has been made of the X-ray crystallography of some eighty sterol derivatives belonging mainly to the cholesterol and ergosterol series but ...Missing: 1950s | Show results with:1950s
[122]
Profiling of Steroid Metabolic Pathways in Human Plasma by GC-MS ...
Feb 13, 2021 · This method provided comprehensive profiling of 20 steroids in human plasma samples and will be helpful to discover potential biomarkers for the development of ...
[123]
GC/MS in Recent Years Has Defined the Normal and Clinically ...
Jul 9, 2018 · GC/MS is excellent at providing an integrated picture of a person's steroid metabolome, or steroidome, as we term it.
[124]
Recent Advances in Liquid Chromatography–Mass Spectrometry ...
Jun 27, 2025 · Recent advancements in HRMS and LC–MS/MS have significantly improved doping detection capabilities by lowering detection limits and enabling ...
[125]
Improving LC-MS/MS measurements of steroids with differential ...
This study provided further evidence that DMS is a powerful complement to established LC-MS/MS instrumentation, improving the specificity of steroid analyses, ...
[126]
Biomolecular NMR spectroscopy in the era of artificial intelligence
Oct 16, 2023 · AI-based tools can dramatically improve the acquisition and analysis of NMR spectra, enhancing the accuracy and reliability of NMR measurements, ...Missing: steroids | Show results with:steroids
[127]
Diosgenin – Knowledge and References - Taylor & Francis
Diosgenin is the most preferred precursor for the commercial synthesis of cortisone, pregnenolone, progesterone and many other steroid drugs in the ...
[128]
Green Manufacturing of Steroids via Mycolicbacteria: Current ... - MDPI
Oct 3, 2023 · In this review, we synthesize some of the recent advancements in steroid biocatalysis using the Mycolicibacterium species.
[129]
Diosgenin: An Updated Pharmacological Review and Therapeutic ...
May 29, 2022 · Diosgenin is a natural compound mostly found in Dioscorea species and starting material for the commercial synthesis of different steroids ...Missing: stigmasterol | Show results with:stigmasterol
[130]
Pathways for the steroidal saponins conversion to diosgenin during ...
Diosgenin is an important starting material for the synthesis of steroidal hormone drugs in pharmaceutical industry. Acid hydrolysis of Dioscorea ...
[131]
Russell Marker Creation of the Mexican Steroid Hormone Industry
Julian's starting material was stigmasterol, a steroid isolated from soybean oil. Learn more about Julian's work by visiting the Percy L. Julian and the ...
[132]
Bioproduction of testosterone from phytosterol by Mycolicibacterium ...
Nov 4, 2022 · Presently, the level of testosterone microbial production from cholesterol is relatively small: its molar yield does not exceed 52% even at ...
[133]
Upjohn Steroid Medicines - American Chemical Society
The only method for preparing this drug prior to 1952 was a lengthy synthesis starting from cholic acid isolated from bile. In 1952, Upjohn biochemists ...
[134]
A Career in Biocatalysis: Kurt Faber | ACS Catalysis
In this review, we examine the mechanisms of several enzymes involved in hydrocarbon biosynthesis, with specific emphasis on the structural and electronic ...<|control11|><|separator|>
[135]
[PDF] A Key Steroid Drug - Longdom Publishing
Nov 20, 2011 · Marker's degradation is the earliest known process for converting diosgenin into 16-DPA [5-7]. As mentioned earlier, acetolysis or ...
[136]
[PDF] Upjohn Progesterone Transformation Team (Murray-Peterson Team)
Jul 15, 2005 · 1950: The Murray-Peterson Team discovers that Rhizopus fungi selectively transform progesterone to 11α-hydroxyprogesterone. The process is ...
[137]
Hydroxylation of Progesterone and Its Derivatives by the ...
Jun 24, 2022 · The discovery of cortisone's anti-inflammatory properties and efficient 11α-hydroxylation catalysed by a Rhizopus species strain has intensified ...Missing: hydrocortisone | Show results with:hydrocortisone
[138]
[PDF] Upjohn Sitosterol Transformation Team (Wovcha Team)
Late 1950's: Upjohn's Greiner developed process for recovering stigmasterol. (~20%) from soy sterols, which concurrently recovers the remaining sterols.Missing: corticosteroids | Show results with:corticosteroids
[139]
Enzymes on Steroids - PMC - NIH
With optimal enzyme variants, whole-cell biotransformations afforded diverse C14α-hydroxylated steroids with 90–95% site selectivity and 72–78% conversion.Missing: semisynthesis 2020s review
[140]
Recent advances in the efficient synthesis of steroid natural products
Jun 11, 2025 · This review presents advances in this field over the past 20 years, highlighting key C–C bond formation and reorganization reactions in the construction of ...
[141]
Recent Progress in Steroid Synthesis Triggered by the Emergence ...
This mini review discusses the recent progress in the synthesis of steroids that was enabled by the advances in the transition metal catalysis, Lewis acid ...
[142]
Robinson Annulation - Organic Chemistry Portal
The Robinson Annulation is a useful reaction for the formation of six-membered rings in polycyclic compounds, such as steroids.
[143]
THE TOTAL SYNTHESIS OF CORTISONE - ACS Publications
A brief encounter on steroids between Robinson and Woodward in 1951. Tetrahedron 2025, 176 , 134516. https://doi.org/10.1016/j.tet.2025.134516. Anindita ...
[144]
Ninety years of progesterone: the 'other' ovarian hormone - PMC - NIH
It is wholly 90 years since the first report of the isolation and characterisation of progesterone by Willard M. Allen and George W. Corner.
[145]
History in medicine: the story of cholesterol, lipids and cardiology
Jan 13, 2021 · In the early 1950s, it became clearer (for some) that there was an association between lipoproteins and cardiovascular disease (CVD) [13].Missing: crystallography | Show results with:crystallography
[146]
https://pmc.ncbi.nlm.nih.gov/articles/PMC9266320/
[147]
The discovery of cortisone: a personal memory - PMC - NIH
Fifty years ago this week, Philip Hench showed that “compound E” (cortisone) was capable of reversing the inflammation of rheumatoid arthritis.
[148]
Elwood V. Jensen (1920–2012): Father of the nuclear receptors - NIH
Mar 4, 2013 · Jensen is popularly credited with the discovery of the mammalian estrogen receptor, and the promulgation of this important molecule as a key player in female ...Missing: 1960s | Show results with:1960s
[149]
A History of Brassinosteroid Research from 1970 through 2005
In this paper, we demonstrate that the brassinosteroid (BR) signal is involved in ovule initiation and development. Ovule and seed numbers are significantly ...
[150]
CRISPR/Cas9‒Mediated Tspo Gene Mutations Lead to Reduced ...
CRISPR/Cas9 was used to introduce Tspo-specific mutations in MA-10 cells. Mutants lost their ability to make steroids owing to reduced mitochondrial membrane ...
[151]
BridgeBio Pharma Reports Topline Results from Phase 1/2 Trial of ...
Sep 10, 2024 · The Phase 1/2 open-label ADventure study was designed to evaluate the safety, tolerability and pharmacodynamic activity of BBP-631 in adults with classic CAH.
[152]
RU486, the anti-pregnancy pill - Lasker Foundation
Feb 27, 2021 · 1989 Albert Lasker Clinical Medical Research Award. RU486, the anti-pregnancy pill. Explore All 1989 Winners & Awards >. Baulieu, Etienne.
[153]
Nuclear hormone receptors for regulating genes - Lasker Foundation
Feb 27, 2021 · The 2004 Albert Lasker Award for Basic Medical Research honors three scientists who opened up the field of nuclear hormone receptor research.
[154]
Role of Epinephrine in the Physiology of Fat Mobilization
Rachmiel Levine. Ira G. Wool. From the Department of Metabolic and Endocrine Research, Medical Research ... Adrenal Steroids and the Liver in the Regulation of ...