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Staphylococcus hominis

Staphylococcus hominis is a Gram-positive, coagulase-negative coccus belonging to the genus Staphylococcus within the family Staphylococcaceae, phylum Bacillota, and is a common commensal bacterium of the human skin microbiota.^[1] These spherical cells, measuring 0.5–1.5 μm in diameter, typically arrange in grape-like clusters and are facultatively anaerobic, catalase-positive, and non-motile, with no spore formation.^[2] As one of the most frequently isolated coagulase-negative staphylococci (CoNS), it thrives in moist, sebaceous, and apocrine areas such as the axillae, groin, and perineum, accounting for up to 22% of staphylococcal skin isolates and persisting for weeks to months.^[3] While generally harmless, S. hominis can act as an opportunistic pathogen, particularly in nosocomial settings, causing bloodstream infections, endocarditis, and sepsis in immunocompromised patients like neonates, cancer sufferers, or those with indwelling devices.^[4] The bacterium exhibits two subspecies: S. hominis subsp. hominis, the more common skin colonizer, and S. hominis subsp. novobiosepticus, notable for its resistance to novobiocin and association with severe infections.^[5] S. hominis demonstrates metabolic versatility, growing optimally at 30–37°C in 0–9% NaCl and pH 4.0–10.0, and is often non-hemolytic with no production of major toxins like enterotoxins or toxic shock syndrome toxin-1 in most strains.^[6] Its cell wall contains peptidoglycan and teichoic acids, contributing to biofilm formation and adhesion to host tissues, which are key virulence factors alongside potential multidrug resistance mediated by genes like mecA.^[7] Beyond its pathogenic potential, S. hominis plays a beneficial role in the skin microbiome by producing autoinducing peptides (AIPs) that interfere with the quorum sensing of Staphylococcus aureus, thereby inhibiting virulence factors and reducing skin damage from opportunistic pathogens.^[3] This protective mechanism, particularly from agr type II strains dominant on healthy skin, has shown promise in preclinical models for preventing atopic dermatitis and S. aureus-induced injuries, with recent 2024 studies confirming benefits to skin barrier and inflammation in atopic dermatitis models.^[8]^[9] High levels of antibiotic resistance, including to methicillin and multiple drugs, underscore the need for vigilant surveillance in clinical isolates, as evidenced by 2025 reports of prevalent multidrug resistance.^[10]^[11]

Taxonomy and Classification

Etymology and History

Staphylococcus hominis was first described as a distinct bacterial species in 1975 by Wesley E. Kloos and Karl-Heinz Schleifer, who isolated it from the skin flora of healthy human subjects as part of a broader study characterizing staphylococci from human skin.^[12] This discovery emerged from systematic sampling of skin sites, revealing S. hominis as a common commensal organism predominantly associated with human hosts, distinguishing it from other staphylococcal species through a combination of morphological, physiological, and biochemical analyses.^[12] The species was formally proposed in their publication, marking a key advancement in understanding the diversity of coagulase-negative staphylococci (CoNS), a group to which S. hominis belongs due to its lack of coagulase production, unlike the pathogenic Staphylococcus aureus.^[12] The etymology of S. hominis reflects its ecological niche: the specific epithet "hominis" derives from the Latin genitive masculine noun meaning "of a human being," underscoring its primary isolation and prevalence on human skin.^[12] This naming convention aligns with the species' characterization as a skin-associated bacterium, often found in areas rich in apocrine glands, such as the axillae.^[12] Early historical milestones included the initial recognition of S. hominis as a non-pathogenic skin resident in the mid-1970s, with Kloos and Schleifer's work highlighting its role in normal human microbiota.^[12] Key studies from this period, such as their 1975 simplified identification scheme, enabled biochemical differentiation from closely related species like S. epidermidis using tests for acid production from carbohydrates (e.g., positive for ribose but variable for others), and ornithine decarboxylase activity. These methods established S. hominis as a distinct CoNS entity, facilitating its separation from S. epidermidis—which is typically novobiocin-sensitive and shows different sugar fermentation patterns—through routine laboratory protocols that emphasized its ecological and clinical distinctions.

Subspecies and Variants

Staphylococcus hominis is divided into two primary subspecies: S. hominis subsp. hominis, which is novobiocin-sensitive, and S. hominis subsp. novobiosepticus, which is novobiocin-resistant.^[13] This distinction was established based on phenotypic and genetic characteristics, with S. novobiosepticus first described in 1998 from clinical isolates associated with infections.^[13] Both subspecies are coagulase-negative, a defining feature of the species, but they exhibit variations in other phenotypic traits such as hemolysis patterns on blood agar, where isolates may show weak beta-hemolysis or non-hemolytic activity depending on the strain.^[14] Biochemical tests further differentiate them, including novobiocin susceptibility as the primary marker, alongside differences in aerobic acid production from D-trehalose and N-acetyl-D-glucosamine, which S. hominis subsp. hominis can utilize while S. novobiosepticus cannot.^[15] Genetic markers provide robust confirmation of these subspecies boundaries. Analysis of 16S rRNA gene sequences reveals a specific single nucleotide polymorphism (SNP) at position 112 (G in S. hominis subsp. hominis and A in S. novobiosepticus), enabling precise differentiation.^[15] Multilocus sequence typing (MLST) using six housekeeping genes (arcC, glpK, gtr, pta, tpiA, and tuf) has identified distinct sequence types (STs), with S. novobiosepticus clustering into a limited number of STs (e.g., ST2, ST16, ST23) showing low diversity, whereas S. hominis subsp. hominis exhibits greater genetic variability across 37 STs.^[16] Additionally, a SNP in the gyrB gene (G431T) correlates perfectly with novobiocin resistance in S. novobiosepticus.^[16] Whole-genome sequencing studies have reinforced these taxonomic subdivisions. For instance, draft genome analysis of S. hominis subsp. hominis strain Hudgins in 2016 confirmed its classification through 16S rRNA identity and absence of certain mobile elements typical of S. novobiosepticus.^[17] Post-2010 genomic investigations, including comparative analyses in 2019 using SDS-PAGE and MALDI-TOF MS profiles, have validated subspecies identification by revealing distinct protein patterns—such as 49 kDa and 85 kDa bands in S. hominis subsp. hominis versus 15 kDa, 26 kDa, 37 kDa, and 90 kDa in S. novobiosepticus—and highlighted higher methicillin resistance in the latter.^[15] These studies underscore the stability of the subspecies boundaries despite ongoing genomic diversity within the species.^[18]

Morphology and Physiology

Cellular Structure

Staphylococcus hominis cells are Gram-positive cocci measuring approximately 1.0 to 1.5 μm in diameter, arranged predominantly in tetrads with occasional pairs or irregular clusters due to successive divisions in multiple planes.^[19] These spherical bacteria are non-motile and non-spore-forming, reflecting their adaptation as commensal skin flora without mechanisms for active dispersal or environmental resistance via endospores.^[19] The cell wall of S. hominis is composed of a thick peptidoglycan layer, which constitutes a significant portion of the cell envelope and provides structural rigidity and protection against osmotic lysis.^[19] This peptidoglycan is of the L-Lys-Gly-L-Ser type, linking amino acids in a cross-bridging network typical of staphylococci.^[19] Embedded within the cell wall are glycerol teichoic acids, poly(glycerol phosphate) polymers partially substituted with 0.3 to 0.8 mol of glucosamine per mol of glycerol, which contribute to ion homeostasis, cell division, and interactions with the host environment.^[19] Notably, the cell wall lacks protein A, a key virulence factor present in Staphylococcus aureus that binds immunoglobulin G to evade phagocytosis, distinguishing S. hominis from its more pathogenic relative.^[20] Under certain stress conditions, S. hominis can produce a variable slime layer, a loosely associated exopolysaccharide matrix resembling a capsule that enhances adherence and biofilm formation on surfaces.^[2] This slime, often composed of teichoic acid derivatives or polysaccharides, is not constitutively expressed but may be induced in nutrient-limited or high-density environments, aiding colonization without rigid encapsulation.^[2]

Metabolic Characteristics

Staphylococcus hominis is a facultative anaerobe, capable of growth under both aerobic and anaerobic conditions, which allows it to thrive in the varying oxygen levels of human skin environments.^[21] It is catalase-positive, producing the enzyme catalase that breaks down hydrogen peroxide into water and oxygen, aiding in the neutralization of reactive oxygen species.^[21] In contrast, it is oxidase-negative, lacking the cytochrome c oxidase enzyme necessary for the oxidation of certain substrates in the oxidase test.^[21] Regarding carbohydrate fermentation, S. hominis ferments glucose, producing acid but no gas, a characteristic shared with other staphylococci that supports energy generation via glycolysis under anaerobic conditions.^[21] Utilization of mannitol and trehalose is variable among strains; for example, acid production from trehalose is often used to distinguish subspecies, with S. hominis subsp. hominis typically positive and subsp. novobiosepticus negative, reflecting adaptations to different ecological niches.^[22] This variability in sugar metabolism contributes to its identification in clinical settings and its ability to exploit diverse carbon sources on the skin.^[23] The enzymatic profile of S. hominis includes positive urease activity, which hydrolyzes urea to ammonia and carbon dioxide, potentially influencing local pH in colonized sites.^[23]

Habitat and Ecology

Natural Reservoirs

Staphylococcus hominis primarily resides as a commensal bacterium on human skin, forming part of the normal cutaneous microbiota. It is particularly prevalent in moist, lipid-rich areas such as the axillae, groin (inguinal folds), and upper body regions like the retroauricular crease. This distribution aligns with the bacterium's preference for environments high in sebum and sweat, which provide nutrients and support its growth. Studies indicate that S. hominis is ubiquitous across healthy individuals, detected in approximately 70% of skin samples from various body sites in cohorts of volunteers, with a mean relative abundance of about 24% among staphylococcal species. It ranks as the second most frequently isolated coagulase-negative staphylococcus (CoNS) from healthy skin, contributing to the microbial balance that helps protect against pathogens.^[24]^[25]^[26] Colonization by CoNS, including S. hominis, tends to be higher in neonates due to the immature skin barrier and altered sebum production that facilitate early establishment, and in the elderly due to reduced antimicrobial peptide activity and changes in skin pH that promote increased staphylococcal colonization, compared to young adults. Sebum production plays a key role in modulating density, as higher levels in sebaceous areas correlate with greater bacterial loads, influencing overall microbiota composition. Overall, S. hominis is a common and often ubiquitous commensal on healthy human skin, detected in the majority of individuals across various body sites, though exact rates depend on sampling site and methodology.^[27]^[28]^[24] While the primary reservoir is human skin, S. hominis has been detected in animal hosts, albeit rarely and not as a dominant species. Isolations have occurred in non-human primates, such as vervet monkeys, where it appears among skin-associated staphylococci, and in livestock like cattle, particularly in bovine milk and udder environments. However, it is not considered a major zoonotic pathogen, with human skin remaining the predominant habitat. Environmental persistence outside hosts is limited; S. hominis, like other staphylococci, can survive on dry inanimate surfaces for weeks under favorable conditions but thrives best in moist, organic niches akin to skin folds.^[29]^[30]^[31]

Transmission and Colonization

Staphylococcus hominis is primarily transmitted through direct skin-to-skin contact, fomites, and healthcare-associated routes, such as contaminated medical devices including catheters and prosthetics.^[32] As a commensal bacterium, it often spreads endogenously from an individual's own skin flora during invasive procedures, but exogenous transmission occurs via healthcare workers' hands in hospital settings.^[33] In neonatal intensive care units (NICUs), nosocomial outbreaks have been documented, with clonal dissemination confirmed through pulsed-field gel electrophoresis (PFGE) analysis of isolates sharing identical restriction patterns.^[33] Colonization by S. hominis begins with adhesion to host skin or abiotic surfaces, facilitated by surface proteins and exopolysaccharides that enable attachment to epithelial cells and medical implants.^[32] Biofilm formation plays a central role in this process, with approximately 50% of clinical S. hominis strains capable of producing biofilms in vitro, primarily composed of polysaccharides (such as poly-N-acetylglucosamine) and proteins, while extracellular DNA contributes minimally.^[34] These biofilms allow persistent colonization on skin sites like the axillae, arms, legs, and inguinal regions, or on indwelling devices, providing protection against host defenses and antimicrobials; in disrupted skin barriers, this can lead to opportunistic overgrowth.^[3]^[32] Key risk factors for S. hominis colonization and subsequent nosocomial spread include immunosuppression, which compromises immune clearance, particularly in neonates and neutropenic patients where CoNS account for 20-40% of septicemia cases.^[32] Hospitalization, especially in ICUs, heightens exposure through invasive devices like central venous catheters, which serve as colonization foci at rates of up to 6.8 infections per 1,000 catheter-days globally.^[32] Prior antibiotic use, such as ampicillin and gentamicin, selects for multidrug-resistant strains, promoting overgrowth and clonal outbreaks in vulnerable populations.^[33]

Pathogenicity and Clinical Relevance

Associated Infections

Staphylococcus hominis, a coagulase-negative staphylococcus (CoNS), is primarily a commensal of human skin but acts as an opportunistic pathogen, particularly in healthcare settings where it causes nosocomial infections.^[35] It is infrequently implicated in disease among healthy individuals but becomes clinically significant in vulnerable populations, leading to bloodstream and device-related infections.^[36] Common manifestations include bacteremia, which accounts for a substantial portion of CoNS bloodstream isolates, often originating from skin flora translocation during invasive procedures.^[35] Bacteremia due to S. hominis frequently presents as catheter-related bloodstream infections (CRBSI), especially in patients with central venous catheters or hemodialysis access.^[37] For instance, in dialysis patients, S. hominis has been isolated from 22.2% of positive blood cultures associated with catheter infections.^[37] Prosthetic joint infections (PJIs) are another recognized association, though S. hominis is less prevalent than S. epidermidis or S. aureus in these cases, contributing to chronic, biofilm-mediated persistence on implants.^[35] Infective endocarditis, typically involving native valves, can occur with embolic complications such as splenic and renal infarcts or spinal discitis, as seen in cases with underlying comorbidities like diabetes and hypertension.^[38] At-risk populations include neonates, where S. hominis is linked to early-onset sepsis and outbreaks in neonatal intensive care units (NICUs), often tied to central lines or skin colonization.^[36] Dialysis patients face elevated risk due to repeated catheter use, with S. hominis implicated in persistent bacteremia requiring prolonged intervention.^[37] Individuals with indwelling devices, such as prosthetic joints, cardiac valves, or vascular grafts, are particularly susceptible, as the organism's biofilm-forming capacity promotes device colonization.^[35] Infections remain rare in immunocompetent adults without procedural breaches.^[39] Clinical symptoms typically involve systemic signs like fever (e.g., 38.2–38.5°C) and chills, alongside localized inflammation such as erythema, warmth, swelling, and tenderness at the infection site.^[40]^[38] In wound-related cases, such as cellulitis overlying surgical hardware, presentations include acute pain, rash, and soft tissue edema, sometimes with polymicrobial involvement complicating the clinical picture.^[40] Abdominal or back pain may signal embolic events in endocarditis.^[38] Diagnostic challenges arise from S. hominis being frequently misidentified as S. epidermidis owing to overlapping biochemical profiles and limitations in automated systems like VITEK 2, which offer low discrimination and necessitate confirmatory tests such as novobiocin susceptibility or carbohydrate fermentation assays.^[36] Multiple positive blood cultures and correlation with clinical symptoms are essential to distinguish true infection from contamination.^[36]

Virulence Factors

Staphylococcus hominis employs several virulence factors that contribute to its pathogenicity, particularly in opportunistic infections associated with medical devices and immunocompromised hosts. These factors enable adhesion, persistence, tissue invasion, and evasion of host defenses, distinguishing it from non-pathogenic commensals despite its primary role as a skin colonizer.^[41] A key virulence mechanism is biofilm production, which facilitates adherence to abiotic surfaces such as catheters and prosthetic devices. This process is primarily mediated by the polysaccharide intercellular adhesin (PIA), a poly-N-acetylglucosamine polymer encoded by the icaADBC operon. In clinical isolates of S. hominis, the ica genes are present in the majority of strains, promoting robust biofilm formation that shields bacteria from antibiotics and host immune clearance. All examined methicillin-resistant S. hominis strains from bloodstream infections demonstrated biofilm production, with adhesion indices to host cells ranging from 4 × 10⁵ to 5.9 × 10⁸ CFU/ml, underscoring the role of PIA and associated exopolymers in device-related persistence.^[42]^[41] S. hominis also produces toxins and enzymes that degrade host tissues and contribute to inflammation. Lipases, which hydrolyze skin lipids like triolein, are secreted by all tested isolates, aiding nutrient acquisition and potentially facilitating colonization of lipid-rich environments such as sebaceous areas.^[35] Additionally, some strains harbor enterotoxin genes, potentially contributing to toxin-mediated syndromes in rare cases.^[43] Extracellular toxins further exhibit cytotoxic effects on epithelial cells, with titres up to 10⁷ in methicillin-resistant strains, leading to cell destruction and enhanced invasiveness.^[35]^[41] Immune evasion is supported by structural components and invasive strategies. Capsular polysaccharides, including poly-γ-DL-glutamic acid synthesized by dedicated genes (capA to capI), form an antiphagocytic layer that reduces opsonization and engulfment by neutrophils and macrophages. Surface-binding proteins further interfere with host recognition by binding complement factors and immunoglobulins, limiting phagocytosis. Moreover, up to 43% of clinical strains invade non-phagocytic epithelial cells, with invasion indices reaching 42%, allowing intracellular survival and dissemination beyond immune surveillance. Biofilms exacerbate this evasion by creating a physical barrier against antimicrobial peptides and effector cells.^[44]^[41]^[41] Acquired genomic elements enhance S. hominis survival and virulence through horizontal gene transfer. Pathogenicity islands carry toxin genes, promoting expression of enterotoxins that disrupt host responses. These mobile elements, analogous to arginine catabolic mobile elements in related staphylococci, bolster metabolic adaptation and persistence in hostile environments such as acidic skin or nutrient-limited sites. Comparative genomics of clinical isolates reveals such islands integrated into the chromosome, contributing to multifactorial pathogenicity.^[35]^[44]^[41]

Identification and Culturing

Laboratory Detection Methods

Laboratory detection of Staphylococcus hominis begins with microscopic examination and Gram staining, which reveals Gram-positive cocci measuring 0.7 to 1.2 μm in diameter, typically arranged in irregular grapelike clusters.^[45] This morphology is characteristic of staphylococci and provides an initial presumptive identification from clinical samples such as blood, skin swabs, or wound exudates.^[2] Biochemical tests are essential for confirming S. hominis as a coagulase-negative staphylococcus (CoNS). The organism is catalase-positive but coagulase-negative, distinguishing it from Staphylococcus aureus.^[2] DNase activity is variable among strains.^[14] Commercial identification systems, such as VITEK 2 or API 20 Staph, are commonly employed for speciating CoNS, including S. hominis, though low discrimination may occur and novobiocin susceptibility testing is recommended for subspecies differentiation.^[46] For subspecies differentiation, novobiocin disk diffusion is widely used: S. hominis subsp. hominis is susceptible (inhibition zone ≥16 mm), while subsp. novobiosepticus is resistant (zone <16 mm).^[36] Molecular methods offer higher specificity and speed for S. hominis identification. Polymerase chain reaction (PCR) targeting the 16S rRNA gene or, preferably, the tuf gene followed by sequencing provides accurate species-level detection, with the tuf gene showing greater discriminatory power than 16S rRNA for CoNS isolates. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) enables rapid proteomic profiling for direct identification from cultures, achieving up to 100% accuracy when combined with tuf sequencing.^[47] Serological methods have limited utility for S. hominis detection due to the absence of specific antibodies or reliable antigens, necessitating correlation with culture-based or molecular confirmation for clinical diagnosis.^[32]

Growth Conditions and Media

Staphylococcus hominis exhibits optimal growth under aerobic or microaerophilic conditions at temperatures of 35–37°C, with abundant colony formation on blood agar within 18–24 hours at 35°C.^[21] Enriched media for growth are typically adjusted to pH 7.0–7.5, while the species tolerates a broader pH range of 4.0–10.0.^[6]^[48] As a facultative anaerobe, it demonstrates robust aerobic growth, though proliferation is reduced under strictly anaerobic environments.^[21] In laboratory settings, S. hominis is routinely cultured on sheep blood agar, yielding small (1–3 mm), low-convex, non-hemolytic colonies after incubation.^[49] On mannitol salt agar, it grows selectively in the presence of 7.5% NaCl but does not ferment mannitol, resulting in opaque white colonies that distinguish it from mannitol-positive species like S. aureus.^[50] Chocolate agar facilitates cultivation of potentially fastidious strains from clinical specimens, while selective media such as Columbia colistin-nalidixic acid (CNA) agar suppress Gram-negative contaminants to enhance isolation of Gram-positive cocci.^[51] Baird-Parker agar, adjusted to pH 6.8–7.2, also supports growth for confirmatory purposes.^[21] Incubation periods of 24–48 hours at 35–37°C are standard for visible colony development, though slow-growing isolates or those in mixed cultures may require extension to 72 hours for reliable enumeration.^[21] Cultivation challenges include diminished growth rates in nutrient-limited media and the propensity for biofilm formation, which can obscure planktonic counts and necessitate mechanical or enzymatic disruption for accurate quantification.^[52]

Antibiotic Resistance

General Resistance Profiles

Staphylococcus hominis exhibits intrinsic resistance to polymyxins as a Gram-positive bacterium lacking lipopolysaccharide targets, and to novobiocin particularly in the subspecies S. hominis subsp. novobiosepticus, due to inherent mechanisms including chromosomal determinants that limit the effectiveness of these agents.^[53]^[13] Additionally, the species commonly demonstrates low susceptibility to penicillin, primarily attributed to the production of beta-lactamases encoded by genes such as blaZ, which hydrolyze the beta-lactam ring and render the antibiotic ineffective.^[54] Among acquired resistance mechanisms, S. hominis frequently harbors genes conferring resistance to macrolides via erm genes, such as erm(C) detected in approximately 58% of clinical isolates, leading to methylation of the ribosomal target and MLS_B phenotype.^[55] Resistance to tetracyclines is similarly prevalent, often mediated by tetK in about 84% of strains, which encodes an efflux pump that expels the antibiotic from the cell.^[55] Multidrug efflux pumps, including msr(A) and msr(B), contribute to broader resistance spectra, affecting over 60% of isolates and enabling expulsion of multiple drug classes.^[55] Recent reviews as of 2025 indicate potential for extensively drug-resistant (XDR) strains resistant to nearly all standard antibiotics, underscoring evolving clinical risks.^[56] Biofilm formation in S. hominis enhances antibiotic tolerance by creating sessile communities where the extracellular matrix reduces penetration of antimicrobial agents, resulting in minimum biofilm eradication concentrations (MBECs) exceeding minimum inhibitory concentrations (MICs) by more than twofold for drugs like vancomycin, linezolid, and ciprofloxacin in over 90% of strong biofilm-producing isolates.^[57] Surveillance of S. hominis susceptibility relies on standardized breakpoints from CLSI and EUCAST guidelines for coagulase-negative staphylococci. For instance, EUCAST defines susceptibility to erythromycin (a macrolide representative) at MIC ≤1 mg/L and resistance at >1 mg/L, while for tetracyclines, susceptible strains have MIC ≤1 mg/L and resistant >1 mg/L; penicillin testing is generally discouraged due to variable beta-lactamase production.^[58] These thresholds guide routine laboratory testing and inform empirical therapy decisions.^[58]

Methicillin-Resistant Strains

Methicillin-resistant Staphylococcus hominis (MRSh) represents a significant portion of clinical isolates from hospitalized patients, with prevalence rates varying by setting but often exceeding 70% among blood culture isolates. For instance, in a study of 21 S. hominis strains from blood samples at a tertiary care hospital in Mexico, 81% were methicillin-resistant. Similarly, analysis of 62 clinical S. hominis isolates from hospitalized patients in Poland revealed that 74% exhibited methicillin resistance. These high rates underscore MRSh as a common opportunistic pathogen in nosocomial environments, particularly associated with bloodstream infections and device-related complications.^[59]^[11] The primary mechanism of methicillin resistance in S. hominis involves the acquisition of the mecA gene, which is integrated into the staphylococcal cassette chromosome mec (SCCmec) mobile genetic element. The mecA gene encodes penicillin-binding protein 2a (PBP2a), a transpeptidase with low affinity for β-lactam antibiotics, thereby preventing effective cell wall synthesis inhibition and conferring resistance to methicillin and other β-lactams. This genetic element is chromosomally located and can vary in structure, with common configurations in MRSh including mec complex A associated with ccrAB1 recombinases, though many isolates harbor non-typeable or novel SCCmec variants. S. hominis often acts as a reservoir for SCCmec components, facilitating their dissemination to other staphylococci via horizontal gene transfer.^[59]^[11]^[26] MRSh isolates typically exhibit low clonality, with pulsed-field gel electrophoresis (PFGE) revealing diverse patterns and limited long-term dissemination within hospitals, as observed in 34 MRSh strains from neutropenic patients where 28 PFGE types were identified. While multilocus sequence typing (MLST) for S. hominis identifies various sequence types, such as ST1 being prevalent in some collections, resistance elements like SCCmec are frequently acquired through horizontal transfer from Staphylococcus aureus or other coagulase-negative staphylococci (CoNS), contributing to the genetic diversity of resistance profiles.^[60] Detection of MRSh relies on both phenotypic and genotypic methods to ensure accurate identification, particularly in clinical laboratories. Phenotypic approaches include the cefoxitin disk diffusion test (30 μg disk, zone diameter ≤21 mm indicating resistance) or oxacillin broth microdilution (minimum inhibitory concentration ≥4 μg/mL), which are recommended for screening coagulase-negative staphylococci due to their reliability in detecting PBP2a-mediated resistance. Genotypic confirmation involves polymerase chain reaction (PCR) targeting the mecA gene, offering rapid and specific detection with sensitivity approaching 100% in cefoxitin-resistant isolates. These methods align with guidelines from the Clinical and Laboratory Standards Institute (CLSI) and European Committee on Antimicrobial Susceptibility Testing (EUCAST), emphasizing the cefoxitin screen for routine use in CoNS.^[61]^[26]^[11]

Epidemiology and Recent Developments

Global Distribution and Outbreaks

Staphylococcus hominis is a common member of the coagulase-negative staphylococci (CoNS) group and is frequently isolated from bloodstream infections worldwide, particularly in healthcare settings. In Asia, it has been reported as the predominant CoNS species in bacteremia cases, accounting for up to 40.8% of isolates in a large cohort from China.^[62] In Europe, prevalence varies but is notable, with S. hominis comprising approximately 6.5% of all speciated CoNS isolates from clinical samples in England between 2010 and 2021, often ranking second or third among CoNS in bacteremia.^[63] Prevalence varies by region, ranging from approximately 6.5% of CoNS isolates in England to 40.8% in some Chinese studies, though its true burden remains underreported in low-resource settings due to limited microbiological surveillance and diagnostic capabilities.^[63]^[62] Notable outbreaks of S. hominis infections have primarily occurred in hospital environments, often linked to nosocomial transmission via contaminated medical devices or surfaces. In the early 2000s, a cluster of invasive infections caused by multidrug-resistant S. hominis subsp. novobiosepticus was identified in a New Jersey hospital in the United States, highlighting its potential as an emerging pathogen in surgical and intensive care patients.^[64] Neonatal intensive care units (NICUs) have been particularly affected, as evidenced by a 2002–2003 outbreak in an Indian hospital where a single clone of S. hominis subsp. novobiosepticus caused sepsis in at least 13 neonates over two years, spread via healthcare worker hands and environmental reservoirs.^[33] Another incident in Brazil in 2006 involved three intensive care patients with bloodstream infections from the same strain, underscoring the role of cross-contamination in outbreaks.^[65] Epidemiological risk mapping reveals higher incidence of S. hominis infections in urban hospitals with extensive use of indwelling devices, such as central venous catheters and ventilators, which facilitate biofilm formation and entry into the bloodstream. Molecular epidemiology studies, often employing pulsed-field gel electrophoresis (PFGE), have demonstrated clonal dissemination in outbreak settings, aiding in source identification and infection control measures.^[33] Recent trends indicate an increasing incidence of S. hominis infections post-2020, attributed to heightened device utilization and prolonged hospitalizations during the COVID-19 pandemic. This rise emphasizes the need for enhanced surveillance in device-heavy clinical environments.

Emerging Research and Challenges

Recent genomic surveillance efforts from 2022 to 2025 have uncovered significant diversity in the staphylococcal cassette chromosome mec (SCCmec) elements among clinical isolates of Staphylococcus hominis, highlighting its role in methicillin resistance among coagulase-negative staphylococci (CoNS). A 2022 comparative genomic analysis of a multidrug-resistant S. hominis strain (ShoR14) isolated from human blood revealed a novel organization of resistance genes, including the mecA gene within an SCCmec type VIII variant cassette, alongside multiple efflux pumps and aminoglycoside-modifying enzymes that contribute to its broad resistance profile.^[4] Similarly, a 2025 study examining 62 clinical S. hominis isolates from various infection sites identified high prevalence of methicillin resistance (up to 80%), with molecular typing showing diverse SCCmec subtypes, including non-typeable variants, underscoring the need for updated surveillance to track evolving resistance mechanisms in hospital settings.^[11] These findings emphasize S. hominis's adaptability, with genomic data revealing intra-species variations that complicate outbreak tracing.^[66] Emerging research into alternative therapies, such as phage therapy, shows promise for combating S. hominis biofilms, though specific trials remain limited compared to other staphylococci. Publications from 2024 and 2025 describe bacteriophage cocktails effective against staphylococcal biofilms in vitro and in animal models, demonstrating up to 90% reduction in biofilm biomass for staphylococcal species, including some CoNS.^[67] However, challenges persist, including misidentification of S. hominis in routine diagnostics due to phenotypic similarities with other CoNS, leading to underreporting and delayed treatment; matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) improves accuracy but requires standardized protocols to distinguish subspecies like S. hominis subsp. hominis and subsp. novobiosepticus.^[68] Limited animal models further hinder progress, as most studies rely on murine skin or sepsis models optimized for S. aureus, which inadequately replicate S. hominis's opportunistic infections in immunocompromised hosts, resulting in variable efficacy data.^[69] Vaccine development faces additional hurdles, with no targeted candidates for S. hominis due to its commensal nature and strain variability, mirroring broader failures in staphylococcal vaccines from immune evasion and poor immunogenicity.^[70] Future directions in S. hominis research leverage artificial intelligence (AI) for resistance prediction and exploration of its role in post-antibiotic microbiome dysbiosis. Machine learning models trained on genomic data have achieved over 95% accuracy in predicting antimicrobial resistance phenotypes for Staphylococcus aureus by analyzing SCCmec and mobile genetic elements, enabling rapid clinical decision-making.^[71] In the skin and gut microbiomes, antibiotic exposure disrupts S. hominis homeostasis, promoting dysbiosis that favors pathogenic overgrowth, as seen in community-acquired infections where its abundance shifts post-treatment, yet this remains understudied outside hospital contexts.^[72] Key gaps include inconsistent subspecies reporting in surveillance databases, which obscures epidemiological patterns, and the need for community-based studies to assess S. hominis's transition from commensal to pathogen in non-clinical settings.^[73] Addressing these through standardized genomic reporting and AI-integrated diagnostics could enhance preventive strategies.^[74]

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Aug 13, 2019 · hominis hominis (Shh) and S. hominis novobiosepticus (Shn). The latter is usually characterized as novobiocin-resistant and unable to form acid ...
[14]
Multilocus Sequence Typing and Further Genetic Characterization of ...
S. hominis was revealed by MLST to be a genetically diverse species, relative to other staphylococci such as S. aureus and S. epidermidis, and recombination ...
[15]
Draft genome sequence of Staphylococcus hominis strain Hudgins ...
Staphylococcus hominis is a predominant member of the human skin microbiome. We here report on the genomic analysis of Staphylococcus hominis strain Hudgins ...2.1. Genome Sequencing... · 3.1. Classification And... · 3.2. Genome PropertiesMissing: studies post-
[16]
Comparative Genomics of Staphylococcus Reveals Determinants of ...
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[17]
Isolation and Characterization of Staphylococci from Human Skin II ...
Jan 1, 1975 · Descriptions of four new species: Staphylococcus warneri, Staphylococcus capitis, Staphylococcus hominis, and Staphylococcus simulans.
[18]
Unbiased Species-Level Identification of Clinical Isolates of ...
Most other CoNS have not been associated with abscesses, due to the absence of the necessary virulence factors coagulase and protein A (13). Although these two ...
[19]
The Genera Staphylococcus and Macrococcus - PMC
Sucrose and fructose are transported exclusively via the PTS system, and PTS plays a central role in trehalose, mannose, maltose, maltotriose, and mannitol ...
[20]
Multilocus Sequence Typing and Further Genetic Characterization of ...
Jun 11, 2013 · Staphylococcus hominis is a commensal resident of human skin and an ... To detect aerobic acid production from D-trehalose and N-acetyl ...
[21]
Five-Test Simple Scheme for Species-Level Identification of ...
The tests included acid production from d-glucose, d-trehalose, d-mannitol ... Staphylococcus epidermidis and Staphylococcus hominis from blood cultures by ...Missing: fermentation | Show results with:fermentation
[22]
Five-Test Simple Scheme for Species-Level Identification of ...
The isolates identified as belonging to the S. epidermidis group, i.e., based on an ornithine decarboxylase-positive test, can be directly identified as S. ...
[23]
Skin-Specific Unsaturated Fatty Acids Boost the Staphylococcus ...
In this study, we questioned whether human skin fatty acids (FAs) can be incorporated into the lipid moiety of lipoproteins and whether such incorporation would ...Missing: nutritional | Show results with:nutritional
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Integrated genomic and functional analyses of human skin ... - PNAS
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The Ubiquitous Human Skin Commensal Staphylococcus hominis ...
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[26]
Antibiotic resistance and mecA characterization of Staphylococcus ...
Feb 7, 2023 · Staphylococcus hominis is one of the major Staphylococcus species found on the human skin and is mainly found in the axillae, perineal and ...3 Results · 3.1 Sociodemographic... · 3.3 Ast Results And Meca
[27]
Early development of the skin microbiome: Therapeutic opportunities
Early life microbial acquisitions may have long lasting effects on health through modulation of host immunity and competitive interactions between bacteria.
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Human Skin Microbiome: Impact of Intrinsic and Extrinsic Factors on ...
The review discusses the relationship between a properly functioning microbiome and the body's immune system, as well as the impact of internal and external ...
[29]
Methicillin-Resistant and Methicillin-Susceptible Staphylococcus ...
Mar 10, 2021 · Here we investigated the presence of methicillin-resistant and susceptible staphylococci in both colony-born and wild vervet monkeys (Chlorocebus sabaeus).
[30]
Genetic diversity and iron metabolism of Staphylococcus hominis ...
This study aimed to extend our knowledge on the genetic diversity and iron uptake capacity of bovine-associated S. hominis originating from different habitats.
[31]
How long do nosocomial pathogens persist on inanimate surfaces ...
The most common nosocomial pathogens may well survive or persist on surfaces for months and can thereby be a continuous source of transmission.
[32]
Coagulase-Negative Staphylococci - PMC - PubMed Central - NIH
A biofilm-defective mutant was shown to lack a 140-kDa surface protein, the accumulation-associated protein Aap. Aap is essential for biofilm accumulation ...
[33]
Nosocomial Spread of a Staphylococcus hominis subsp ... - NIH
32 isolates of Staphylococcus hominis subsp. novobiosepticus (SHN) were recovered from 21 patients, 18 of whom were neonates, with 13 considered to have late- ...Missing: elderly | Show results with:elderly
[34]
https://doi.org/10.1007/s12223-014-0332-4
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Staphylococcal species less frequently isolated from human clinical ...
Feb 11, 2020 · In the literature, S. epidermidis and S. aureus are described as the most common causes of prosthetic joint infections (PJIs), that are one of ...Missing: presentations | Show results with:presentations
[36]
Staphylococcus hominis subsp. novobiosepticus, an emerging ... - NIH
Staphylococcus hominis is one of the three most frequently identified isolates recovered from bloodstream infections (BSIs)1. In 1998, characterization of subsp ...
[37]
Daptomycin, Methicillin Resistant Staphylococcus hominis Catheter ...
We report a case of a haemodialysis patient that presented a catheter-related bacteraemia caused by a Coagulase negative Staphylococcus. With the utilization of ...Missing: neonates devices
[38]
Staphylococcus hominis Infective Endocarditis Presenting with ...
May 14, 2022 · Staphylococcus hominis (S. hominis) is a Gram-positive, coagulase-negative bacteria that occurs as a normal commensal organism on the skin ...Missing: characteristics | Show results with:characteristics
[39]
Transient Metabolic Acidosis in a Healthy Toddler - PubMed Central
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[40]
Staphylococcus hominis cellulitis and bacteremia associated ... - NIH
Feb 1, 2022 · We present a case of cellulitis at the site of a healed surgical incision with underlying surgical clips, with associated Staphylococcus hominis bacteremia.
[41]
Multifactorial mechanisms of the pathogenesis of methicillin ... - NIH
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[42]
Biofilm formation by Staphylococcus hominis strains isolated from ...
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[43]
Optimization of physical parameters for enhanced production of ...
The attempts have been made to improve the production of lipase from Staphylococcus hominis MTCC 8980 by optimization of pH, temperature, and agitation speed.
[44]
Complete genome sequencing of three human clinical isolates of ...
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[45]
Staphylococcus Hominis - an overview | ScienceDirect Topics
Species of staphylococci are separated into two large groups on the basis of ability to produce the extracellular enzyme coagulase. Organisms that produce ...
[46]
https://pmc.ncbi.nlm.nih.gov/articles/PMC1829067/
[47]
Staphylococcus - an overview | ScienceDirect Topics
Staphylococci grow readily on laboratory media growing between 12°C and 44°C with an optimum of 37°C at an optimum pH of 7.5. After 24 hours, colonies are 1–3 ...
[48]
[PDF] ID 7 - Identification of Staphylococcus species, Micrococcus species ...
May 26, 2023 · They are opportunistic pathogens which lack many of the virulence factors associated with S. aureus. There are more than 30 species of CoNS. The ...<|separator|>
[49]
22A: Identification of Staphylococcus Species - Biology LibreTexts
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[50]
Reservoirs of Nosocomial Pathogens in Intensive Care Units - NIH
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[51]
Staphylococcal Biofilms: Challenges and Novel Therapeutic ...
Mixed biofilms substantially increase the complexity of the infection, by altering the antimicrobial susceptibilities of the species involved and making ...
[52]
https://pmc.ncbi.nlm.nih.gov/articles/PMC7911828/
[53]
The prevalence of multidrug resistance in Staphylococcus hominis ...
Jan 2, 2025 · The aim of the study was to determine antibiotic resistance in 62 strains S. hominis isolated from clinical materials, and to identify the molecular basis of ...Methods · Tested Strains · Discussion
[54]
Antibiotic Susceptibility of Biofilm Cells and Molecular ... - NIH
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[55]
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Summary of each segment:
[56]
Microbiological and Molecular Characterization of Staphylococcus ...
In conclusion, our results showed that S. hominis is a biofilm producer and in combination with its high resistance rate to antibiotics, renders this species a ...
[57]
The prevalence of multidrug resistance in Staphylococcus hominis ...
Jan 2, 2025 · S. hominis is one of the three CoNS species most frequently isolated from patients with bloodstream infections. S. epidermidis is often reported ...Missing: taxonomy | Show results with:taxonomy
[58]
Molecular Epidemiology of Methicillin-Resistant Staphylococcus ...
Jul 8, 2011 · The S. hominis population showed a limited clonality and a low genetic diversity in the allotypes of ccr and classes of mec complex.Results · Optimization Of Pfge Typing... · Figure 1. Pfge Profiles...
[59]
Laboratory Testing for Methicillin (oxacillin)-resistant ... - CDC
Jun 27, 2025 · Key points · There are many methods laboratorians can use to test for MRSA. · Four phenotypic methods are recommended for the detection of MRSA.Oxacillin/methicillin... · Susceptibility Test Methods · Interpreting Results
[60]
The species distribution, antimicrobial resistance and risk factors for ...
Among the 157 patients, the top two common species of CoNS were Staphylococcus hominis (40.8%) and Staphylococcus epidermidis (36.3%). This result was ...Missing: global | Show results with:global
[61]
https://www.cdc.gov/mrsa/php/laboratories/index.html
[62]
Epidemiology and Pathogenesis of Staphylococcus Bloodstream ...
Overall, the infections are grouped into skin and soft tissue infections (SSTIs) with manifestations such as dermatitis, abscesses, furunculosis, boils, ...Missing: presentations | Show results with:presentations
[63]
A sequence variant of Staphylococcus hominis with a high ...
A newly identified subspecies of Staphylococcus hominis, S. hominis subsp. novobiosepticus, was found to be the cause of several invasive infections at a ...
[64]
The species distribution, antimicrobial resistance and risk factors for ...
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[65]
Outbreak of Staphylococcus hominis subsp. novobiosepticus ...
Feb 1, 2008 · Outbreak of Staphylococcus hominis subsp. ... hospitals: summary of results from three years of the SENTRY Antimicrobial Surveillance Program.
[66]
COVID-19 pandemic increased ESKAPEEc bloodstream infections ...
Oct 19, 2025 · Staphylococcus epidermidis (26.9%) was the most frequently isolated bacteria, followed by Staphylococcus hominis (17.7%), E. coli(6.6%), ...
[67]
Draft genome sequences of Staphylococcus species isolated from ...
Sep 30, 2025 · To further investigate these species, we sequenced the genomes of four isolates from voided urine samples collected from asymptomatic (“healthy”) ...
[68]
Efficacy of phage therapy in controlling staphylococcal biofilms
Jul 9, 2025 · This systematic review examined the effectiveness of bacteriophages against biofilms created by antibiotic- and drug-resistant staphylococcal strains.Missing: hominis | Show results with:hominis
[69]
Current Limitations of Staph Infection Diagnostics, and the Role for ...
S. epidermidis is an important human commensal but is also capable of deadly infections. Gold-standard diagnostic methods for staph infections currently rely ...
[70]
A systematic review of animal models for Staphylococcus aureus ...
While no ideal animal model exists, the understanding of a model's strengths and limitations should assist clinicians and researchers to appropriately select an ...
[71]
Challenges for Clinical Development of Vaccines for Prevention of ...
Unpredictable attack rates and changing epidemiology as well as highly variable genetic and immunological strain characteristics complicate the development.Missing: hominis | Show results with:hominis
[72]
Prediction of antimicrobial resistance in Staphylococcus aureus with ...
Sep 2, 2025 · In our study, we developed a machine learning (ML) model that reliably predicts the antimicrobial resistance (AMR) phenotypes of ...
[73]
Alterations of human skin microbiome and expansion of ... - Science
Dec 22, 2021 · Consistent with culture-based evidence, our current work demonstrates that antibiotics can disrupt the homeostasis of skin microbial communities ...
[74]
Staphylococcal species less frequently isolated from human clinical ...
Feb 11, 2020 · In our studies, almost all S. haemolyticus isolates as well as S. hominis isolates produced haemolysis of sheep blood. It is in opposition to ...Missing: hemolysis | Show results with:hemolysis
[75]
Artificial intelligence in predicting pathogenic microorganisms ...
This article reviews the latest advancements in AI and ML for predicting antimicrobial resistance in pathogenic microorganisms.Missing: hominis dysbiosis<|separator|>