Fertility testing
Fertility testing encompasses a range of medical evaluations and diagnostic procedures designed to identify underlying causes of infertility, defined as the inability to conceive after one year of regular unprotected intercourse in couples under 35 years old, or after six months in those 35 and older.[1] Globally, approximately 1 in 6 people of reproductive age experience infertility in their lifetime.[2] These tests assess reproductive health in both partners, evaluating factors such as ovulation, sperm quality, tubal patency, and ovarian reserve, to guide potential treatments like assisted reproductive technologies.[3] Infertility affects approximately 1 in 5 (19%) married women aged 15-49 with no prior births in the United States, with causes attributable to female factors (about 30-40%), male factors (30-40%), or combined/unexplained issues (20-30%).[1][4] Evaluation typically begins with a thorough medical history and physical examination, followed by targeted laboratory and imaging tests tailored to each partner's symptoms.[3] For women, common assessments include ovulation testing via midluteal serum progesterone levels to confirm ovulatory function, ovarian reserve testing using anti-Müllerian hormone (AMH) levels (normal >1.66 ng/mL) or antral follicle count (AFC >4 follicles via transvaginal ultrasound), and hysterosalpingography (HSG) to detect fallopian tube blockages with dye under X-ray (sensitivity 65%, specificity 83%).[4] Additional female tests may involve thyroid function screening, prolactin levels, and pelvic ultrasound to evaluate uterine abnormalities.[3] For men, semen analysis remains the cornerstone, measuring sperm concentration (normal ≥15 million/mL), motility (≥40%), and morphology (≥4% normal forms), often requiring two samples for accuracy.[4] Hormone testing for testosterone, follicle-stimulating hormone (FSH), and prolactin, along with genetic evaluations like karyotyping for low sperm counts (<10 million/mL), may follow abnormal results.[3] Scrotal ultrasound can identify structural issues such as varicoceles.[3] Guidelines from organizations like the American Society for Reproductive Medicine (ASRM) recommend initiating testing after 12 months for those under 35, six months for ages 35-40, and immediately for those over 40, emphasizing early intervention to improve outcomes.[4] While most tests are non-invasive, some like laparoscopy (gold standard for tubal assessment) involve minor surgery.[4] Overall, fertility testing enables personalized management, with success rates varying by age and diagnosis—over 80% of couples conceive within one year naturally, but testing can accelerate resolution for the rest.[4]Overview
Definition and Purpose
Fertility testing encompasses a systematic series of medical evaluations aimed at identifying factors that may impair conception, including assessments of hormonal levels, structural integrity of reproductive organs, and functional aspects of gamete production and transport.[5][6] According to the American Society for Reproductive Medicine (ASRM) 2023 committee opinion, infertility is defined as a disease, condition, or status characterized by: (1) the inability to achieve a successful pregnancy based on a patient's medical, sexual, and reproductive history, age, physical findings, diagnostic testing, or relevant proteins/steroid biochemistry in accordance with evidence-based thresholds; (2) the inability to carry a pregnancy to live birth; or (3) the need for fertility services to achieve a successful pregnancy.[6][1][2] This updated definition promotes inclusivity for diverse populations, including LGBTQ+ individuals, single parents, and those not engaging in heterosexual intercourse, ensuring broader access to evaluation and care. The core purpose of fertility testing is to diagnose underlying reproductive issues, thereby informing personalized treatment strategies such as hormonal therapies, intrauterine insemination, or assisted reproductive technologies (ART) like in vitro fertilization (IVF), while also offering reassurance to individuals with normal fertility and facilitating preconception counseling to optimize future reproductive health.[5][7] For instance, tests like semen analysis or anti-Müllerian hormone (AMH) levels may briefly indicate potential concerns, guiding further intervention without exhaustive procedural details.[5] Over the 20th century, fertility testing evolved from rudimentary techniques, such as basal body temperature charting for ovulation tracking and early semen microscopy first introduced in 1677 by Antonie van Leeuwenhoek, to the advent of IVF in 1978, which integrated diagnostic insights with laboratory-based reproduction.[8][9] By the early 21st century, advancements in biomarkers, genetic screening, and imaging have further refined these methods, enhancing their role within comprehensive ART frameworks as of 2025.[9] Globally, infertility impacts approximately 1 in 6 people of reproductive age, affecting an estimated 17.5% of the adult population and underscoring the need for accessible testing.[10][2] For individuals or couples under 35 years old, fertility testing is typically recommended after 12 months of unsuccessful attempts at conception through unprotected intercourse.[11][7]Indications for Testing
Fertility testing is primarily indicated for individuals or couples experiencing infertility, which may later be classified as unexplained if no cause is identified after evaluation.[5] This guideline, established by the American Society for Reproductive Medicine (ASRM), aims to facilitate early intervention to optimize outcomes, as fertility declines with age and delays can reduce success rates.[5] For women over 40, evaluation may be initiated sooner than the standard timelines due to the accelerated decline in ovarian reserve and higher risks of chromosomal abnormalities.[5] Similarly, the American Urological Association (AUA) and ASRM advise concurrent evaluation of the male partner in all infertile couples, following the same 12- or 6-month timelines based on the female partner's age.[12] Testing is also warranted earlier in the presence of known risk factors that may impair fertility. In women, these include irregular menstrual cycles (such as oligomenorrhea or amenorrhea), history of pelvic infections, endometriosis, polycystic ovary syndrome (PCOS), or prior pelvic surgeries that could lead to adhesions or tubal damage.[5][1] For men, indications encompass clinical varicocele, genital tract infections (e.g., epididymitis), or occupational exposures to heat, chemicals, or toxins, which can adversely affect spermatogenesis.[12][13] These factors prompt prompt assessment to identify treatable causes and prevent further fertility compromise. Lifestyle and environmental triggers further heighten the need for testing, including smoking, excessive alcohol use, obesity, and advancing paternal age over 40, which is associated with increased risks to offspring health.[1] Trends toward delayed parenthood, driven by socioeconomic factors and improved access to contraception, have amplified demand for fertility evaluations as more individuals pursue conception later in life.[14] Additionally, preconception testing is recommended for high-risk groups, such as carriers of genetic disorders, to screen for conditions that could affect reproductive success or offspring health.[15]Assessment in Women
Ovulation Detection Methods
Ovulation detection methods encompass a range of non-invasive and minimally invasive techniques designed to identify the timing and occurrence of ovulation, which is crucial for optimizing conception efforts or diagnosing conditions like anovulation. These approaches leverage physiological changes in body temperature, hormone levels, cervical characteristics, and ultrasound imaging to pinpoint the fertile window, typically spanning the 24-48 hours around ovulation. Home-based methods empower individuals to track cycles affordably, while clinical options provide higher precision for fertility evaluations.[16] Basal body temperature (BBT) charting involves recording daily morning temperatures immediately upon waking, before any activity, to detect the biphasic shift that occurs post-ovulation due to rising progesterone levels. This shift, usually an increase of 0.5 to 1°F (0.3 to 0.6°C), confirms ovulation has happened but does not predict it in advance, limiting its utility for timing intercourse. Accuracy can be affected by factors such as illness, stress, alcohol consumption, or inconsistent measurement, with studies indicating retrospective identification of the ovulatory period in about 80-90% of cycles when charted properly.[17][18] Ovulation prediction kits (OPK) are urine-based tests that detect the luteinizing hormone (LH) surge, which precedes ovulation by 24-36 hours, allowing prospective identification of the fertile window. These kits, available over-the-counter from brands like First Response or Clearblue, measure LH levels comparable to serum thresholds and achieve detection accuracy of 90-99% when used correctly, though false positives can occur in conditions like PCOS. Sensitivity varies by brand, with thresholds typically around 25-30 mIU/mL for positive results, making them a reliable home tool for most women with regular cycles.[19][20][16] Electronic fertility monitors, such as the Clearblue Fertility Monitor, enhance OPK functionality by analyzing urine samples for both estrogen and LH levels across multiple days, identifying up to six fertile days per cycle with 99% accuracy in hormone detection. These devices provide digital readouts of fertility status (low, high, peak) and integrate with smartphone apps for data tracking and cycle predictions as of 2025 models featuring touch screens and Bluetooth connectivity. Clinical validation supports their use in natural family planning, though they require daily testing during the expected fertile period and may be costlier than basic kits.[21][22] Cervical mucus observation, including the stretch test (also known as the spinnbarkeit test), monitors changes in vaginal discharge throughout the cycle to infer ovulation. Mucus transitions from sticky and opaque in the pre-ovulatory phase to clear, slippery, and stretchy (resembling raw egg whites) during the fertile window, facilitating sperm survival and transport. The Billings Ovulation Method formalizes this by emphasizing vulvar sensations and mucus patterns without internal examination, achieving typical-use effectiveness of 78-97% for fertility awareness when taught properly. This method relies on daily observations after being upright for several hours, with peak fertility indicated by slippery mucus lasting 1-2 days around ovulation.[23][24][25] Serum progesterone testing serves as a biochemical confirmation of ovulation via a blood draw, typically in the mid-luteal phase (around cycle day 21 for a 28-day cycle), where levels exceeding 5 ng/mL indicate corpus luteum formation post-ovulation. This threshold reflects adequate progesterone production to support potential implantation, with values below suggesting anovulation or luteal phase deficiency. While highly specific (positive likelihood ratio of about 2.8 for levels ≥6 ng/mL), timing is critical, and serial measurements may improve accuracy over single tests in irregular cycles.[26][27][28][29] Calendar and sympto-thermal methods combine cycle length tracking with multiple signs, such as BBT, cervical mucus, and sometimes cervical position, to delineate fertile and infertile phases. Apps like Natural Cycles, validated by the FDA in 2018 as a birth control method, use algorithms incorporating BBT data to predict ovulation with 93% typical-use effectiveness, though efficacy drops with irregular cycles or user error. These approaches promote comprehensive fertility awareness but require consistent daily logging for reliability.[30][31][32] Daily transvaginal ultrasound offers precise clinical monitoring of follicular development, where a dominant follicle grows to 18-25 mm before ovulation, confirmed by its rupture or disappearance on subsequent scans. Performed in fertility clinics starting around cycle day 8-10, this method visualizes real-time ovarian changes with near-100% accuracy for timing ovulation, particularly useful in assisted reproduction. It is minimally invasive but involves a probe insertion, limiting it to medical settings.[33][34] Cervical position tracking complements other signs by noting the cervix's changes: it rises, softens (like the tip of a nose), and opens slightly near ovulation due to estrogen influence, contrasting its low, firm, closed state post-menstruation. Observed manually by inserting clean fingers, this sign peaks 1-2 days before ovulation and enhances accuracy when combined with mucus or BBT observations, though individual variability and hygiene concerns may affect ease of use.[35][36][37]Ovarian Reserve Evaluation
Ovarian reserve evaluation assesses the quantity and quality of a woman's remaining oocytes, providing insights into potential response to fertility treatments such as assisted reproductive technologies. This evaluation is essential for women over 35 or those with risk factors like prior ovarian surgery, as diminished reserve correlates with reduced fertility potential. Key methods include hormonal assays and ultrasound imaging, with interpretations guided by age-specific norms that reflect the natural decline in ovarian function beginning around age 30 and accelerating after 35. According to the 2024 ESHRE guideline on premature ovarian insufficiency, ovarian reserve markers like anti-Müllerian hormone (AMH) can predict the risk of early reserve loss, emphasizing the importance of timely testing. Interpretation of AMH levels should account for assay-specific variability, as different platforms may yield differing results.[38][39] Anti-Müllerian hormone (AMH) testing involves a simple blood draw that measures levels produced by granulosa cells in pre-antral and small antral follicles, serving as a proxy for the antral follicle count and overall ovarian pool. Unlike other hormones, AMH levels remain stable across the menstrual cycle, allowing testing on any day, which enhances accessibility. For women aged 25-35, normal AMH ranges from 1-4 ng/mL, with levels below 1 ng/mL indicating diminished reserve and poorer response to ovarian stimulation. The 2025 ESHRE ovarian stimulation guideline highlights AMH's utility in tailoring controlled ovarian hyperstimulation protocols, as low levels predict fewer oocytes retrieved during IVF.[40][39] Cycle day 3 follicle-stimulating hormone (FSH) testing evaluates basal pituitary-ovarian axis function early in the follicular phase, where elevated FSH signals the pituitary's attempt to recruit follicles from a depleted reserve. Levels exceeding 10 IU/L on day 3 suggest diminished ovarian reserve, often combined with estradiol measurement to improve accuracy; ideally, estradiol should be below 80 pg/mL to avoid masking subtle FSH elevations. The American Society for Reproductive Medicine (ASRM) 2020 committee opinion recommends this test for women with suspected low reserve, noting its prognostic value for IVF outcomes despite assay variability across labs.[39] The clomiphene citrate challenge test (CCCT) dynamically assesses ovarian responsiveness by administering 100 mg of clomiphene citrate daily from cycle days 5-9, followed by FSH measurement on day 3 (baseline) and day 10. A day 10 FSH level above 10 IU/L indicates poor reserve, as it reflects inadequate follicular recruitment under stimulation. This provocative test is more sensitive than basal FSH alone for detecting subtle declines, particularly in women aged 35-40, according to a comprehensive review in Endotext.[41] Antral follicle count (AFC) uses transvaginal ultrasound to enumerate follicles measuring 2-10 mm in both ovaries during the early follicular phase (days 2-5), providing a direct visual estimate of recruitable follicles. Counts below 5-7 bilaterally signify low reserve and predict limited oocyte yield in IVF cycles. The ASRM endorses AFC for its low interobserver variability and complementary role with hormonal tests, as outlined in their 2020 opinion on ovarian reserve measures.[39] Inhibin B, a heterodimeric glycoprotein secreted by granulosa cells, serves as a less commonly used biomarker of early follicular development, with day 3 levels below 45 pg/mL associated with diminished reserve and poor ovarian response. While historically promising, its clinical adoption is limited due to greater assay variability compared to AMH or AFC. A 2021 systematic review confirms inhibin B's correlation with other markers in reproductive-age women but notes its secondary role in modern guidelines.[42] The 2025 ESHRE guidelines integrate these tests with age-specific interpretations, recommending combined use (e.g., AMH plus AFC) for robust counseling on fertility preservation options, as reserve decline varies individually but follows a predictable trajectory post-35.[40]Uterine and Fallopian Tube Assessment
Uterine and fallopian tube assessment is a critical component of fertility evaluation in women, focusing on anatomical integrity to identify blockages, structural anomalies, or abnormalities that may impair sperm transport, egg retrieval, or embryo implantation.[43] These evaluations primarily utilize imaging modalities to visualize the uterine cavity and tubal patency without invasive intervention, helping to guide treatment decisions such as in vitro fertilization (IVF) or surgical correction.[44] Common indications include unexplained infertility, recurrent pregnancy loss, or suspected pelvic inflammatory disease sequelae.[45] Hysterosalpingography (HSG) is a radiographic procedure that involves injecting iodinated contrast dye through the cervix under fluoroscopy to outline the uterine cavity and assess fallopian tube patency.[46] It reveals the shape of the uterus, detecting anomalies like fibroids or adhesions, and determines if tubes are open by observing dye spillage into the peritoneal cavity.[45] Typically performed in the follicular phase post-menses to avoid pregnancy, HSG carries risks such as pelvic infection (occurring in about 1-3% of cases), allergic reactions to the dye, or uterine perforation, though severe complications are rare.[47][44] Hystero-contrast sonography (HyCoSy) employs transvaginal ultrasound with a contrast agent, such as saline mixed with air or foam, to evaluate tubal flow and uterine morphology in real time.[48] The procedure allows visualization of contrast movement through the tubes, confirming patency if it spills beyond the fimbriae, and offers advantages over HSG including no ionizing radiation exposure and immediate results without need for X-ray equipment.[43] Studies indicate HyCoSy has comparable accuracy to HSG for tubal occlusion detection, with sensitivity and specificity exceeding 80-90% in infertile populations.[49] Saline infusion sonohysterography (SHG), also known as sonohysterography, uses transvaginal ultrasound augmented by instilling sterile saline into the uterine cavity to expand and delineate endometrial contours.[50] This enhances detection of intrauterine abnormalities such as polyps, submucosal fibroids, or synechiae that may disrupt implantation, distinguishing intracavitary lesions from myometrial ones with high specificity (up to 95%).[51][52] Performed outpatient with minimal discomfort, SHG is particularly useful in infertility workups for women with irregular bleeding or prior failed implantations.[53] Hystero-foam sonography (HyFoSy) is an ultrasound-based variant using a stable foam contrast (e.g., ExEm Foam) for tubal patency assessment, providing clear visualization of foam transit through the tubes and into the pelvis.[54] It offers similar diagnostic accuracy to HSG and HyCoSy, with meta-analyses reporting sensitivity around 90% and better tolerability due to reduced pain.[55][56] Basic transvaginal ultrasound serves as an initial, non-invasive screening tool to identify gross uterine anomalies, such as bicornuate or septate uterus, which affect up to 5-10% of infertile women and can compromise implantation.[33] It also measures endometrial thickness—ideally 7-14 mm in the luteal phase for optimal receptivity—flagging thin linings associated with poor fertility outcomes.[57] This modality provides a baseline assessment before more specialized tests.[58] Interpretation of these tests distinguishes proximal tubal blockages (near the uterine cornua, often from salpingitis isthmica nodosa) from distal ones (at the fimbriae, commonly due to adhesions), with proximal occlusions accounting for 10-25% of tubal infertility cases.[59] Hydrosalpinx, a dilated tube filled with fluid indicating distal obstruction, prevails in 10-20% of infertile women and negatively impacts IVF success by up to 50% if untreated.[60] While imaging suffices for diagnosis, advanced endoscopy like hysteroscopy may confirm findings in complex cases.[61]Advanced Diagnostic Procedures
Advanced diagnostic procedures in fertility testing encompass invasive techniques that enable direct visualization and intervention within the female reproductive tract, particularly when non-invasive methods such as hysterosalpingography (HSG) yield inconclusive results. These approaches are essential for confirming structural abnormalities, staging conditions like endometriosis, and facilitating immediate treatments to optimize fertility outcomes, often integrating seamlessly with assisted reproductive technology (ART) planning.[5] Hysteroscopy involves the insertion of a thin endoscope through the cervix to inspect the uterine cavity, allowing for the identification of intrauterine pathologies such as polyps, fibroids, or adhesions that may impede implantation. Diagnostic hysteroscopy focuses on visualization, while operative hysteroscopy enables concurrent interventions like polyp removal or adhesiolysis to restore cavity integrity and potentially improve conception rates. This procedure is frequently performed in an office setting using local anesthesia and saline distension, reducing the need for general anesthesia and enabling same-day recovery with minimal discomfort. Complication rates for diagnostic hysteroscopy are low, ranging from 0.012% to 1.65%, with uterine perforation being the most frequent adverse event, though it rarely requires further intervention.[5][62][63] Laparoscopy with chromotubation provides comprehensive surgical assessment of the pelvic cavity and fallopian tubes by introducing a laparoscope through small abdominal incisions under general anesthesia, combined with the injection of a colored dye via the cervix to evaluate tubal patency through observed spillage at the fimbrial ends. This technique serves as the gold standard for staging endometriosis, detecting subtle peritoneal adhesions, or confirming tubal blockages not fully elucidated by prior imaging, thereby guiding decisions on fertility-preserving surgeries. It is particularly valuable for distinguishing treatable causes of infertility, such as mild endometriosis, which may benefit from excision to enhance natural or ART conception. Complication rates for laparoscopic procedures in infertility contexts typically range from 1% to 2%, encompassing risks like infection, bleeding, or visceral injury, with most cases managed conservatively.[5][64] Three-dimensional (3D) sonography enhances transvaginal ultrasound by generating multiplanar reconstructions of the uterus, enabling precise mapping of congenital anomalies such as septate or bicornuate uteri that could compromise embryo implantation. This modality offers diagnostic accuracy comparable to magnetic resonance imaging for differentiating anomaly types, such as measuring indentation depth (1.0-1.5 cm) to distinguish arcuate from septate configurations, and is routinely recommended pre-IVF to inform whether corrective procedures like metroplasty are needed. Beyond basic imaging, advanced ovarian ultrasound employs Doppler to assess vascularity, revealing increased blood flow in pathological lesions, while characteristic features like homogeneous low-level echoes or ground-glass appearance identify endometriomas or functional cysts that may affect ovarian function. These ultrasound refinements provide non-surgical yet detailed insights into adnexal pathology, supporting targeted interventions.[65][66] These procedures are indicated after abnormal HSG findings, such as suspected tubal occlusion, or in persistent infertility cases where non-invasive tests fail to identify a cause, allowing for definitive diagnosis and integration into ART protocols to address barriers like uterine irregularities or pelvic adhesions before cycles commence. Risks generally involve anesthesia-related issues for laparoscopy and minor procedural events for hysteroscopy, with recovery times varying from hours for office-based hysteroscopy to 1-2 days for laparoscopy, often permitting outpatient discharge.[5]Assessment in Men
Semen Analysis
Semen analysis is the cornerstone of male fertility evaluation, providing a direct assessment of sperm production, quality, and function through examination of ejaculated semen. This laboratory test measures various parameters to identify potential issues contributing to infertility, such as low sperm count or poor motility. It is recommended for men experiencing difficulty conceiving after 12 months of unprotected intercourse or in cases of known risk factors like varicocele or prior infections.[67][68] The procedure begins with the patient abstaining from ejaculation for 2-7 days to ensure a representative sample, followed by collection via masturbation into a sterile, wide-mouthed container, ideally at the laboratory to minimize contamination and allow immediate analysis. The sample is then allowed to liquefy for 20-30 minutes at 37°C before evaluation. If collection at home is necessary, the sample must be transported to the lab within 1 hour, kept at body temperature.[67] Key parameters assessed include semen volume, sperm concentration, total sperm count, motility, morphology, vitality, pH, and the presence of white blood cells, with lower reference limits established by the World Health Organization (WHO) based on the 5th centile from fertile men. These values help classify semen quality but are not diagnostic alone, as fertility depends on multiple factors. The following table summarizes the WHO 2021 lower reference limits:| Parameter | Lower Reference Limit |
|---|---|
| Semen volume | ≥1.4 mL |
| Sperm concentration | ≥15 × 10⁶ per mL |
| Total sperm number | ≥39 × 10⁶ per ejaculate |
| Progressive motility | ≥30% |
| Total motility | ≥40% |
| Morphology (normal forms) | ≥4% |
| Vitality (live spermatozoa) | ≥58% |
| pH | ≥7.2 |
| White blood cells | <1 × 10⁶ per mL |