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GraphPad Software

GraphPad Software is a scientific software company founded in 1989 by Dr. Harvey Motulsky, a pharmacologist and biostatistics expert, with a focus on developing intuitive tools for data analysis, statistics, and graphing tailored exclusively for the international research community.^[1] Its flagship product, Prism, is a versatile, no-coding-required platform that enables scientists to organize data in structured tables, perform guided statistical tests such as t-tests, ANOVA, and regression, and generate customizable publication-quality visualizations.^[2] Used by over 750,000 researchers across more than 110 countries, including institutions like the CDC, Novartis, and the University of Oxford, Prism emphasizes accuracy, efficiency, and collaboration through features like cloud integration.^[2] Originally headquartered in San Diego, California, and later relocated to Boston, Massachusetts, GraphPad has prioritized user feedback from scientists to refine its offerings, with Motulsky also authoring the influential book Intuitive Biostatistics to complement the software's educational approach.^[1] The company underwent a majority recapitalization in 2017 with Insight Venture Partners, which supported its growth and led to the 2019 acquisition of SnapGene, a molecular biology tool, forming Insightful Science.^[3]^[4] In 2021, Insightful Science merged with Dotmatics, a broader scientific informatics platform, creating a unified entity under the Dotmatics name by 2022 to enhance end-to-end R&D workflows for over 2 million global scientists.^[5] This integration expanded GraphPad's ecosystem while keeping Prism as a standalone, unchanged tool serving biotechnology, pharmaceuticals, academia, and healthcare sectors.^[6] In July 2025, Siemens AG completed its $5.1 billion acquisition of Dotmatics, incorporating GraphPad's capabilities into Siemens' AI-powered life sciences portfolio to accelerate innovation in research and development software.^[7] Through these developments, GraphPad continues to empower non-statisticians in the sciences by simplifying complex analyses and promoting reproducible results.^[8]

History

Founding

GraphPad Software was founded in 1989 in La Jolla, California, by Dr. Harvey Motulsky, a pharmacologist serving on the faculty of the Department of Pharmacology at the University of California, San Diego.^[1]^[9] The company began as a privately held software development corporation dedicated to producing specialized tools for the international scientific community.^[10]^[11] Motulsky, drawing from his background in pharmacology and biostatistics education, established GraphPad to address the challenges faced by life sciences researchers in analyzing and visualizing data without advanced statistical expertise.^[1]^[12] The initiative was driven by a commitment to create intuitive software "by scientists for scientists," simplifying statistical analysis and graphing for non-statisticians in fields like biology and medicine.^[6] The company's name derives from Motulsky's pre-founding project, InPlot, an early scientific graphing program released in September 1984 under the title "Graph-PAD," which influenced GraphPad's focus on accessible data presentation tools.^[13] This foundational software set the stage for GraphPad's evolution into a key provider of scientific software solutions.

Early development

GraphPad Software's early development featured the launch of foundational products tailored to scientific data visualization and analysis. The company's inaugural software, Graph-PAD, was released in September 1984 as a DOS-based graphing program, later rebranded as InPlot following the formal establishment of GraphPad in 1989.^[13] This tool provided essential plotting capabilities for researchers, marking the beginning of GraphPad's commitment to accessible scientific software. In the early 1990s, GraphPad introduced InStat, a straightforward program for basic biostatistical tests, designed specifically for scientists lacking extensive statistical training, which guided users through data analysis with step-by-step prompts.^[14] A pivotal milestone came in June 1994 with the release of GraphPad Prism version 1.00, an integrated platform combining graphing and statistical analysis for biomedical research.^[13] Unlike the standalone tools that preceded it, such as InPlot and InStat, Prism evolved into a unified solution that streamlined workflows by embedding nonlinear regression, curve fitting, and visualization directly within a single interface, reducing the need for multiple programs. This innovation addressed the growing demand among life scientists for intuitive tools that minimized technical barriers while supporting complex data interpretation. Throughout the 1990s and 2000s, GraphPad refined its portfolio with additional releases, including StatMate in June 2004, which focused on power analysis and sample size calculations to aid experimental design.^[13] As a privately held company dedicated exclusively to the scientific community since its inception, GraphPad emphasized user-friendly interfaces that empowered non-experts, fostering international adoption starting in the 1990s. By the 2010s, this focus had propelled significant growth, with the software serving over 750,000 scientists across 110 countries.^[1]^[15]

Acquisitions and mergers

In 2017, GraphPad Software underwent a majority recapitalization led by Insight Partners, marking a shift from its status as a fully private entity to one backed by significant venture capital investment, which provided resources for expanded growth in scientific software development.^[16]^[3] The year 2019 saw GraphPad expand through strategic acquisitions, beginning with Biomatters in April, the developer of Geneious Prime bioinformatics software, to bolster its offerings in molecular biology analysis.^[17] Later that August, GraphPad acquired SnapGene, a leading molecular biology documentation tool, which facilitated the formation of Insightful Science as a parent company overseeing a portfolio that included GraphPad Prism, SnapGene, and Geneious Prime, aiming to create an integrated life sciences software ecosystem.^[4]^[18] In March 2021, Insightful Science merged with Dotmatics, a provider of cloud-based scientific R&D data management platforms, in a transaction valued at approximately $690 million, forming a larger entity under the Dotmatics name and backed by Insight Partners; this positioned GraphPad as a key brand within the combined company's broader scientific informatics suite.^[19]^[20]^[21] As of November 2025, GraphPad operates as part of Dotmatics, which was acquired by Siemens AG in July 2025 for $5.1 billion, maintaining its independent branding particularly for Prism while integrating into Siemens' expanded AI-driven life sciences portfolio; this structure has preserved GraphPad's distinct identity without dissolution.^[7]^[22]^[23] These corporate events have enhanced GraphPad's operational resources, enabling accelerated product development and seamless integrations with Dotmatics' tools, such as Luma, an AI platform for multimodal R&D workflows that connects Prism data for advanced insights and automation.^[24]^[25]^[26]

Products

GraphPad Prism

GraphPad Prism is a commercial proprietary software developed by GraphPad Software for scientific data analysis, graphing, and statistics. Released in 1994, it serves as the company's flagship product, designed to streamline workflows for researchers handling complex datasets.^[13] The software is available on macOS and Windows platforms, ensuring compatibility across common operating systems used in scientific environments. As of September 2025, the latest version is 10.6.1, which includes enhancements for stability and user interface improvements.^[27]^[8] Prism operates on a subscription-based licensing model, with individual plans starting at $260 per user per year for academic users, alongside corporate options at $520 per year and student pricing at $142 annually. Enterprise licensing provides customized solutions, including single sign-on and premium support, while a free 30-day trial is available without requiring a credit card.^[28] At its core, Prism features an integrated architecture centered on specialized data tables tailored for scientific experiments, such as XY tables for precise measurements, column tables for grouped data, and contingency tables for categorical analyses. This structure facilitates direct data entry and organization without external spreadsheets. The software supports batch processing for automating repetitive tasks and scripting for advanced automation, enabling efficient handling of large-scale experiments.^[8] Primarily targeted at the life sciences field, including biology, pharmacology, and medicine, Prism excels in producing publication-quality graphs and outputs that meet journal standards, helping researchers visualize and communicate findings effectively.^[8]

GraphPad QuickCalcs

GraphPad QuickCalcs is a free, web-based statistical calculator suite developed by GraphPad Software as a companion tool to its primary product, Prism, enabling users to perform basic analyses directly in a web browser without software installation.^[29] Accessible at quickcalcs.graphpad.com, it offers over 25 specialized calculators designed for rapid computations in scientific research and education.^[2] The tool's key functionalities focus on essential statistical tests and calculations, including t-tests for comparing means, chi-square and Fisher's exact tests for categorical data, linear regression for correlation and basic curve fitting, confidence intervals, P-value computations, and sample size estimation.^[30]^[31] Additional options cover descriptive statistics, outlier identification, multiple comparisons post-tests, distribution analyses (such as binomial, Poisson, and Gaussian), data simulation, random group assignment, and chemical calculations like molarity conversions and radiochemical decay.^[29] Unlike full-featured software, QuickCalcs does not support graphing, data storage, or import/export of datasets, requiring manual entry of values for each calculation.^[29] Its primary purpose is to provide on-the-fly statistical support for researchers lacking access to Prism, as well as for educational purposes among students learning basic biostatistics.^[32] The interface promotes simplicity, with results displayed immediately alongside explanations of assumptions and interpretations to aid non-experts.^[33] Limitations include the absence of advanced analyses, such as nonlinear regression or complex modeling, making it unsuitable for in-depth studies; it also features promotional links encouraging upgrades to Prism for enhanced capabilities.^[34] QuickCalcs serves as an entry point to the GraphPad ecosystem, attracting thousands of scientists and students daily for routine tasks, with popular calculators like the molarity tool and t-test calculator driving its accessibility.^[2]

GraphPad Prism

Purpose and design

GraphPad Prism is a statistical analysis and graphing software package designed primarily for scientists in the life sciences, such as biologists and pharmacologists, who require accessible tools for data analysis without possessing advanced statistical expertise.^[2]^[35]^[36] It targets researchers in fields like pharmacology and experimental biology, enabling them to perform analyses on experimental data while avoiding the complexities of professional statistical software.^[8]^[37] The software's design philosophy emphasizes an intuitive user interface and guided workflows to minimize errors and streamline the process from data entry to visualization, following an "analyze and graph" paradigm. Built-in help resources, checklists, and tutorials, including the Prism Academy, support users through each step, making it suitable for non-experts. Common use cases include analyzing experimental data, such as dose-response curves and survival analysis, as well as preparing publication-ready figures for scientific journals.^[8]^[38]^[39] Unlike general-purpose tools like spreadsheets or programming-based software, Prism is specifically tailored for scientific data types, including repeated measures and nonlinear models, and eliminates the need for coding to perform analyses. This focus on domain-specific workflows distinguishes it by automating routine tasks and integrating analysis directly with graphing.^[8]^[2] Prism's design has evolved significantly since its initial release as version 1.00 in June 1994, which offered basic graphing and analysis capabilities for early adopters in medical and pharmaceutical research. Subsequent versions introduced enhanced navigation, expanded data organization options, and more sophisticated visualization tools, culminating in version 10 (released in 2023 with updates through 2025), which features a more open file format, interactive graph customization panels, and integrated cloud synchronization via Prism Cloud for collaborative workflows.^[13]^[40]^[41]

Statistical analysis capabilities

GraphPad Prism provides a comprehensive suite of built-in statistical tools tailored for scientific data analysis, enabling researchers to perform parametric and nonparametric tests, regression analyses, and specialized computations without requiring external software.^[8] These capabilities are integrated into an intuitive workflow, where analyses are selected via checklists that guide users through assumptions and options, ensuring appropriate application for biological and medical datasets.^[42] Common analyses in Prism include t-tests for comparing means between two groups, available in unpaired, paired, and nested variants with options for multiple comparisons corrected by false discovery rate (FDR) or Bonferroni methods.^[8] One-way ANOVA assesses differences across multiple groups, while two- and three-way ANOVA (with limitations on factor levels) evaluate interactions between independent variables, including repeated measures via mixed-effects models.^[42] For data not meeting normality assumptions, nonparametric alternatives such as the Mann-Whitney test for two independent groups and the Kruskal-Wallis test for more than two groups are supported, providing robust comparisons without parametric requirements.^[8] Regression tools in Prism encompass linear regression for straight-line relationships, including simple, multiple, and Deming variants, as well as advanced nonlinear curve fitting with over 100 built-in equations.^[43] Nonlinear fitting is particularly emphasized for scientific modeling, supporting equations like the logistic model for binary outcomes and the Michaelis-Menten equation for enzyme kinetics, where substrate concentration (X) relates to velocity (Y) via Y = V_{\max} \frac{X}{K_m + X}, with V_{\max} as maximum velocity and K_m as the Michaelis constant.^[44] Model comparison is facilitated through Akaike information criterion (AIC) and Bayesian information criterion (BIC), allowing users to select the best-fitting equation based on parsimony and goodness-of-fit.^[43] Advanced features extend to survival analysis with Kaplan-Meier curves for estimating survival probabilities over time and log-rank tests for comparing survival distributions between groups.^[42] Dose-response analysis computes metrics like EC50 (effective concentration for 50% response) and IC50 (inhibitory concentration) using nonlinear regression on log-transformed concentrations.^[43] Contingency table analyses include chi-square tests for independence in categorical data and Fisher's exact test for small samples, aiding in assessing associations in clinical or experimental outcomes.^[42] Validation and automation enhance reliability, with automatic computation of error bars (e.g., SEM, CI) and handling of replicates through dedicated data tables that average or pool values as needed.^[8] Built-in checks verify assumptions, such as normality via Anderson-Darling or D'Agostino tests, and identify outliers using methods like Grubbs' or ROUT, prompting users to transform data or switch to nonparametric approaches if violations occur.^[42] A cornerstone of Prism's nonlinear regression is the four-parameter logistic (4PL) model for sigmoidal dose-response curves, defined as:

Y = \text{Bottom} + \frac{(\text{Top} - \text{Bottom})}{1 + 10^{(\text{LogEC}_{50} - X) \cdot \text{HillSlope}}}

where Y is the response, X is the logarithm of the dose or concentration, Bottom and Top are the lower and upper asymptotes, LogEC50 is the log concentration producing 50% of the maximal response (derived as EC50 = 10^{LogEC50}), and HillSlope describes the curve's steepness (positive for agonists, negative for antagonists).^[44] To fit this model, users enter X-Y data (with X as log(concentration)) into an XY table, select the "Dose-response -- Variable slope (four parameters)" equation, provide initial parameter values if needed, and run the analysis, which iteratively minimizes residuals using the least-squares method.^[43] Interpretation involves assessing parameter confidence intervals for precision, residual plots for fit quality, and derived values like EC50 with symmetry or asymmetry based on HillSlope; a HillSlope near 1 indicates standard sigmoidality, while deviations suggest cooperative or non-cooperative binding.^[45] This model is widely used in pharmacology for potency estimation, with Prism automating interpolation and uncertainty propagation for robust scientific inference.^[43]

Graphing and data visualization

GraphPad Prism provides a wide array of graph types to visualize scientific data, including XY plots for continuous data, bar and column graphs for categorical comparisons, survival curves using Kaplan-Meier estimation, contour and heat maps for multivariate patterns, violin plots for data distributions, and receiver operating characteristic (ROC) curves for diagnostic performance assessment.^[46]^[47]^[48]^[49]^[50]^[51] With over 20 predefined styles accessible via the Change Graph Type dialog, users can select thumbnails representing diverse visualizations tailored to experimental needs.^[52] Customization in Prism is extensive, allowing real-time adjustments through the Graph Inspector for elements like axis scaling (linear, log, or semilog), error bars representing standard error of the mean (SEM), standard deviation (SD), or confidence intervals (CI), annotations, and predefined themes.^[53]^[54] Users can modify data point shapes, colors, fonts, labels, and layouts to create multi-panel figures, ensuring graphs align with specific analytical or aesthetic requirements.^[55]^[56] For publication, Prism supports high-resolution exports in formats such as TIFF (up to 1200 dpi), EPS, and PDF, facilitating seamless integration into journals or presentations.^[57] Features include color-blind friendly palettes, automatic legends, and labeling to enhance accessibility and clarity.^[58] One-click export to PowerPoint or email further streamlines sharing.^[8] Prism enables batch graphing by applying consistent styles across multiple datasets, with tools for interpolation, smoothing, and overlaying graphs to generate composite visualizations.^[59] Unique capabilities include heat maps for clustered grouped data, which use color gradients to highlight patterns, and ROC curves that plot sensitivity against specificity for evaluating diagnostic tests.^[49]^[60]

Data management and collaboration

GraphPad Prism facilitates data entry through a variety of structured tables designed to accommodate different experimental designs, including XY tables for paired data, column tables for single variables, grouped tables for two-way comparisons (such as treatment and gender), and nested tables for hierarchical structures like subgroups within main groups.^[8]^[61] These tables support up to 256 datasets and subcolumns, allowing users to enter quantitative and categorical data directly while handling missing or excluded values appropriately for subsequent analyses.^[61] For importing external data, Prism supports formats such as Excel (.xls/.xlsx), CSV, and its native .pzf files, with options to paste or import directly into specific table structures while preserving column titles and formatting.^[8] Additionally, it enables direct integration with laboratory instruments via scripts that import data from text files generated by devices, streamlining workflow from acquisition to analysis.^[62]^[63] Data organization within Prism emphasizes linkage and documentation to maintain integrity across projects. Analyses are inherently linked to their source data tables, ensuring that modifications to the data automatically update all associated results, graphs, and layouts without manual intervention.^[8] Each sheet, whether a data table or analysis, can include dedicated Info sheets for structured metadata—such as dates, concentrations, or lot numbers—and unstructured notes, which serve as constants that can be referenced in transforms, regressions, or graph elements.^[64]^[8] Up to 500 Info sheets per project allow for comprehensive annotation, with options to link them globally or to specific tables for contextual relevance.^[64] Collaboration features were significantly enhanced with the introduction of Prism Cloud in version 10, released on July 11, 2023, providing a web-based platform integrated with the desktop application for seamless sharing and co-editing.^[40] Users can publish projects to Prism Cloud for real-time viewing and discussion, enabling team members to provide feedback via threaded comments without emailing files, while co-editing supports simultaneous modifications by eligible subscribers.^[41]^[65] Version history is maintained through iterative publishing, allowing reversion to prior states, and enterprise workspaces facilitate integrations for larger organizations by organizing shared folders and role-based access.^[66]^[40] Export capabilities in Prism cover raw data, results tables, and graphs in multiple formats, including CSV for data, TIFF/PDF for visuals (with customizable resolution and color modes), and JSON for structured outputs to ensure interoperability.^[8] An API and scripting system enable automation of imports, analyses, and exports, allowing batch processing of large datasets or integration with other software.^[67] By default, Prism stores projects locally on the user's device for full control, but shared cloud projects employ end-to-end encryption, secure coding practices, and role-based permissions to protect sensitive research data.^[68]^[8]

Versions and updates

GraphPad Prism's version history reflects ongoing enhancements to its core functionalities in statistical analysis, graphing, and data handling. The software's first major release, version 1.0 in 1994, introduced basic integration of graphing and statistical tools for scientific data visualization and analysis. Subsequent iterations built on this foundation, with version 5 launched in 2007 featuring improved regression capabilities that streamlined curve fitting and model comparisons.^[69] Version 8, released on October 16, 2018, marked significant advancements in nonlinear regression, achieving up to 1.5 times faster performance compared to version 7 while enhancing accuracy for complex models.^[13]^[70] Version 10, introduced on July 11, 2023, incorporated a more open .prism file format to facilitate better data sharing and integration with external systems.^[40]^[71] In 2025, development continued with frequent updates to address user needs and incorporate emerging technologies. Integration with Dotmatics Luma for seamless data management across platforms was introduced in version 10.4.0, released on October 29, 2024.^[72] Version 10.6.0, released on August 20, 2025, added automatic cluster number selection for K-means clustering to simplify unsupervised learning tasks.^[13]^[73] This was followed by version 10.6.1 on September 23, 2025, which focused on bug fixes, including resolutions for clustering visualizations and stability issues in statistical tests.^[74] GraphPad provides free minor updates to subscribers during their active subscription period, ensuring access to the latest features and fixes without additional cost; major version upgrades typically require a purchase or subscription renewal for perpetual license holders.^[75] The software emphasizes backward compatibility, with newer versions able to open and edit files from prior releases, though advanced features introduced in later versions may appear limited or require updates when opened in older software.^[76] Releases follow a pattern of annual major updates paired with quarterly minor ones, allowing steady refinement based on user feedback and technological advances. Looking ahead, GraphPad plans to prioritize AI automation for predictive analytics, expanded cloud collaboration tools, and deeper interoperability with Dotmatics ecosystem products like Luma to support integrated scientific workflows.^[27]^[71]

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You can plot multiple ROC curves on one graph if you want to. The easiest way to do so is to go to a graph of one ROC curve, and drag the "ROC curve" results ...
[61]
Grouped tables (two grouping ... - GraphPad Prism 10 User Guide
A two-grouping variable table and graph is used to tabulate outcomes that are measurements such as weight or blood pressure, often with error bars.Missing: structured | Show results with:structured
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Script example 1. Repeatedly import data - GraphPad
This script analyzes a folder full of dose-response data stored as text files, perhaps from an instrument. It creates a single file that tabulates the ...
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How can Prism be integrated with other programs? How can I create ...
Using this approach, you can effectively (from the user's point of view) save a Prism file from your application (or instrument). No scripts. No need to use ...
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Using Info sheets to organize your ... - GraphPad Prism 10 User Guide
Use Info sheets to record experimental details and constants you want to use in analyses. The left side of an info sheet is a table of structured information.Missing: metadata | Show results with:metadata
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GraphPad Prism 10 User Guide - Prism Cloud Co-Editing
Prism Cloud is only available with eligible subscriptions and in specific regions. Co-Editing allows team members to collaborate on the same Prism project by ...
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Workspaces - GraphPad Prism 10 User Guide
The Prism Cloud workspace is a common, shared place for workspace members to publish, share, and create discussions to collaborate on their projects created ...
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GraphPad Prism 10 User Guide - Overview of Prism scripts
Use a script to import data into an existing project and to specify which results and graphs you want to export or print. Scripts are small computer programs.
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How Prism Cloud Protects Your Data - FAQ 2236 - GraphPad
Prism Cloud encrypts data, uses secure coding, monitors with tools, isolates data, enforces role-based access, and does not sell user data.
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Graph prism version 5, by GraphPad - Product details - Pubcompare
Is this product still available? Get pricing insights and sourcing options. Spelling variants (same manufacturer). GraphPad Prism 5 ... release (2007), publisher ...
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Prism 8 Release Notes: What's New & Improvements - GraphPad
Oct 15, 2018 · Non-linear regression is 1.5x faster than in Prism 7; Exact nonparametric tests (Mann-Whitney, Kruskall-Wallis, Spearman correlation ...Missing: nonlinear | Show results with:nonlinear
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Dotmatics Launches GraphPad Prism 10 with a More Open File ...
The new, more open Prism file format will allow for a future integration into Luma™️, helping scientists and administrators in life sciences unify and analyze ...
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Prism 10.6.0 - GraphPad
Automatic Cluster Number Selection for K-means clustering. Take the guesswork ... Analyze, graph and present your scientific work easily with GraphPad Prism.
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Prism 10.6.1 - GraphPad
Sep 23, 2025 · Removed the labels on the PC scores graph of the "K-means clustering (Wine)" sample data ... Analyze, graph and present your scientific work ...
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Which are the current versions of Prism? How can I update or ...
You can find the latest version of GraphPad Prism in our Updates page. Before Updating Please determine your Prism license type and current version.
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Can files I created with one version of Prism be opened by another?
Every Prism version can open older files, and older versions can generally open newer files, but some features may not work. Windows and Mac versions use the ...