Fact-checked by Grok 2 weeks ago

MADS-box

The MADS-box is a family of genes that encode transcription factors characterized by a highly conserved DNA-binding domain, known as the MADS domain, which typically spans about 56 amino acids and recognizes specific DNA motifs called CArG-boxes (CC[A/T]₆GG).^[1] The acronym "MADS" derives from the names of the four founding members of this gene family: MCM1 (from the yeast Saccharomyces cerevisiae), AGAMOUS (from the plant Arabidopsis thaliana), DEFICIENS (from the snapdragon Antirrhinum majus), and SRF (serum response factor from humans).^[1] These genes are found across eukaryotes, including fungi, animals, and plants, but have undergone extensive diversification and expansion in land plants, where they number over 100 in many angiosperm species due to ancient duplications dating back to the common ancestor of land plants.^[2] Structurally, MADS-box proteins are classified into two main types: Type I (M-type), which lack a keratin-like domain and are involved in gametophyte and seed development, and Type II (MIKC-type), which include additional intervening (I), keratin-like (K), and C-terminal (C) domains that facilitate protein dimerization, multimerization, and transcriptional regulation.^[1] In plants, MIKC-type MADS-box genes are particularly prominent and function as key regulators of developmental processes, most notably in the specification of floral organ identity through the ABC(DE) model, where specific combinations of proteins like APETALA1, APETALA3, PISTILLATA, and AGAMOUS determine sepals, petals, stamens, and carpels.^[3] Beyond flowers, these genes control fruit ripening, seed and embryo morphogenesis, root architecture, vegetative growth transitions, and even responses to abiotic stresses such as drought and salinity by modulating hormone signaling and gene networks.^[4] Evolutionarily, the MADS-box family traces its origins to a duplication event in the topoisomerase IIA subunit A gene in early eukaryotes, with subsequent radiations enabling adaptations in plant morphology and reproductive strategies across seed plants.^[2]

Nomenclature and History

Etymology

The term "MADS-box" is an acronym derived from the names of four founding genes that share a conserved DNA-binding domain: MCM1 (Minichromosome maintenance 1) from the yeast Saccharomyces cerevisiae, AGAMOUS from the plant Arabidopsis thaliana, DEFICIENS from the plant Antirrhinum majus, and SRF (serum response factor) from mammals. This nomenclature was first proposed in 1990 by Schwarz-Sommer et al. in their analysis of plant homeotic genes, highlighting the structural and functional similarities among these transcription factors across eukaryotes.^[5] MCM1 encodes a transcription factor that regulates cell-type-specific gene expression in yeast, serving as a key mating-type regulator.^[6] AGAMOUS is a plant gene that specifies floral organ identity, particularly for stamens and carpels.^[7] DEFICIENS functions as a homeotic gene controlling flower morphogenesis in Antirrhinum.^[8] SRF acts as a mammalian transcription factor that binds to the serum response element in promoters to mediate immediate-early gene responses.^[9]

Research Milestones

The discovery of MADS-box genes began in the late 1980s with the isolation of key members in yeast and mammals. In 1987, the MCM1 gene was identified in the budding yeast Saccharomyces cerevisiae as a transcription factor essential for mating-type-specific gene expression and minichromosome maintenance. Shortly thereafter, in 1988, the serum response factor (SRF) was cloned from humans as a DNA-binding protein that activates immediate-early genes in response to serum stimulation. These findings highlighted a conserved DNA-binding motif in eukaryotic transcription factors. In plants, the first MADS-box genes were isolated from floral homeotic mutants in 1989. The AGAMOUS (AG) gene was cloned from Arabidopsis thaliana, revealing its role in specifying stamen and carpel identity, with the encoded protein showing homology to transcription factors.^[7] Concurrently, the DEFICIENS (DEF) gene was identified in snapdragon (Antirrhinum majus), where it controls petal and stamen development, and its protein sequence exhibited similarity to MCM1 and SRF. The 1990s marked the formal recognition of the MADS-box as a conserved DNA-binding domain. In 1990, the term "MADS-box" was coined from the initials of MCM1, AG, DEF, and SRF, based on sequence comparisons that defined a 58-amino-acid motif responsible for DNA binding and protein dimerization. This period also saw the proposal of the ABC model of floral organ identity in 1991, which linked MADS-box genes like APETALA1 (A-class), APETALA3 and PISTILLATA (B-class), and AG (C-class) to the specification of sepals, petals, stamens, and carpels in whorled arrangements. During the 2000s, advances in genomics enabled comprehensive identifications of MADS-box genes across species. In 2000, following the Arabidopsis genome sequencing, 107 MADS-box genes were cataloged, revealing their expansion and diversification in flowering plants. Similar efforts in rice (Oryza sativa) identified 75 MADS-box genes by 2007, classifying them into type I and type II subgroups and highlighting orthologs to floral regulators.^[10] Research also expanded to non-plant organisms, confirming MADS-box presence in animals and fungi, with studies tracing an ancestral duplication predating the plant-animal divergence. In the 2010s and 2020s, structural biology and applied genomics advanced MADS-box research. A 2014 crystallographic study elucidated the oligomerization mechanism of the MADS-domain K domain from SEPALLATA3 (SEP3), showing how it facilitates protein interactions essential for higher-order complexes in gene regulation.^[11] More recently, a 2024 review synthesized genomic analyses of MADS-box genes in rice, emphasizing their regulation of yield traits like panicle architecture and grain filling through interactions in developmental networks.^[12] In 2025, studies on MADS-box genes in ferns revealed dynamic evolution via large-scale duplications, further illuminating diversification in non-seed plants.^[13]

Protein Structure

MADS Domain

The MADS domain is a highly conserved DNA-binding and dimerization motif comprising approximately 58 amino acids, characteristic of MADS-box transcription factors found throughout eukaryotes.^[1] This domain enables specific interactions with target DNA sequences and protein oligomerization, serving as the foundational structural element for the regulatory functions of these proteins.^[1] Structurally, the MADS domain folds into a helix-turn-helix configuration, featuring an N-terminal extension of about 14 amino acids, a prominent amphipathic α-helix responsible for major groove insertion and sequence-specific DNA recognition, an intervening turn, and two C-terminal β-strands that form an antiparallel β-sheet for dimerization.^[14]^[1] The α-helix makes direct base contacts primarily in the major groove, while the N-terminal extension facilitates interactions with the minor groove and the DNA phosphate backbone, contributing to overall DNA bending upon binding.^[14] The intervening turn links the α-helix to the β-strands, stabilizing the domain's architecture and positioning for dimer formation.^[14] The binding specificity of the MADS domain centers on recognition of the CArG-box motif in target DNA, with the consensus sequence CC(A/T)_6GG.^[1] This 10-base-pair element, often embedded in promoter regions, allows the dimeric domain to contact symmetric half-sites, inducing moderate DNA bending to facilitate transcriptional regulation.^[14] Sequence conservation of the MADS domain is remarkably high across eukaryotic lineages, including plants, animals, and fungi, with core residues exhibiting substantial similarity that preserves the structural fold and binding properties despite functional diversification.^[1] This evolutionary stability underscores the domain's ancient origin and essential role in diverse developmental processes.^[2]

Additional Structural Features

In addition to the core MADS domain, type II MADS-box proteins typically feature a keratin-like (K) domain, consisting of approximately 70 amino acids, which adopts a leucine zipper-like structure characterized by three α-helices connected by short loops.^[15] This motif facilitates protein dimerization through hydrophobic interactions and enables the formation of higher-order complexes, such as tetramers observed in floral organ identity proteins like SEPALLATA3.^[16]^[17] The C-terminal domain varies in length and sequence across MADS-box proteins but generally serves as a regulatory region involved in transcriptional activation or repression.^[18] In many plant MADS-box proteins, such as those in the MIKC type, this domain contains glutamine-rich motifs that function as activation domains, promoting target gene expression through interactions with co-activators.^[19] Structural studies of MADS-box proteins, including X-ray crystallography of the SEPALLATA3 K domain and NMR analyses of related domains, reveal an overall fold with flexible intervening (I) linkers between the MADS and K domains, allowing conformational adaptability for multimer assembly.^[17]^[16] These linkers, typically 25–30 amino acids long, contribute to the dynamic nature of the protein architecture.^[16] Post-translational modifications, particularly phosphorylation at serine (Ser) and threonine (Thr) residues within or near the K domain, modulate protein stability and activity in MADS-box factors.^[20] For instance, phosphorylation of OsMADS23 by the kinase SAPK9 at specific Ser/Thr sites enhances its protein stability, thereby influencing stress responses without altering DNA-binding affinity.^[20]

Classification and Diversity

Type I MADS-box Genes

Type I MADS-box genes encode transcription factors characterized by the presence of only the conserved MADS domain, typically 58-60 amino acids in length, and the absence of the K-box domain found in type II genes.^[21] In the Arabidopsis thaliana genome, approximately 67 such genes have been identified, classified into four subfamilies: Mα (25 genes), Mβ (20 genes), Mγ (16 genes), and Mδ (6 genes).^[21] These genes generally produce shorter proteins of around 200 amino acids, with transcripts often comprising one or two exons and few or no introns, reflecting their simpler genomic organization.^[1] Sequence conservation is high within the MADS domain but notably lower in the regions outside it, contributing to their structural variability across subfamilies.^[21] Type I MADS-box genes exhibit a broad distribution across eukaryotes, occurring ubiquitously in animals, fungi, and plants.^[1] In animals, they align with SRF-like genes, which regulate serum response and contribute to processes such as muscle cell differentiation.^[22] As an ancient evolutionary lineage predating the emergence of type II genes, type I MADS-box genes participate in core cellular regulatory functions and display a higher rate of birth-and-death evolution compared to their type II counterparts.^[23]

Type II MADS-box Genes

Type II MADS-box genes, also known as MIKC-type genes, are distinguished by their characteristic domain architecture comprising the N-terminal MADS domain, an intervening (I) region, a K-box domain, and a C-terminal domain.^[16] This structure enables specific protein-protein interactions and DNA binding, setting them apart from the simpler Type I genes.^[24] In the model plant Arabidopsis thaliana, approximately 45 MIKC-type genes have been identified through genome-wide analyses.^[25] These genes are further subdivided into two main subtypes: MIKC^c (or MIKC^C) and MIKC. The MIKC^c subtype predominates in plants and includes genes such as APETALA1 (AP1), which is involved in specifying floral organ identity.^[26] In contrast, the MIKC subtype, which is less abundant, plays roles in processes like root and seed development, with members forming distinct phylogenetic clades.^[27] In plants, Type II MADS-box genes exhibit significant expansions, particularly in lineages with complex genomes, driven by gene duplications following whole-genome duplication events. For instance, in the grass Oryza sativa (rice), 44 MIKC-type genes have been annotated, reflecting diversification beyond the basal eudicot count.^[28] A recent genome-wide study in flax (Linum usitatissimum) identified 114 total MADS-box genes, including expanded MIKC clusters attributed to polyploidy and segmental duplications.^[29] Outside plants, Type II MADS-box genes are present but show limited diversification. In animals, representatives like the human MEF2C gene belong to this category, functioning in developmental processes such as myogenesis and neural crest specification, with typically only a few copies per genome.^[24] Fungal homologs are similarly sparse, lacking the extensive subtype elaborations seen in plants.^[2]

Biological Functions

Roles in Plant Development

MADS-box genes play pivotal roles in specifying floral organ identity in angiosperms through the ABC(DE) model, where combinatorial interactions of specific transcription factors determine the development of sepals, petals, stamens, and carpels. A-class genes, such as APETALA1 (AP1), primarily promote sepal formation and floral meristem identity when acting alone, while their combination with B-class genes like APETALA3 (AP3) and PISTILLATA (PI) specifies petals; B-class genes alone with C-class gene AGAMOUS (AG) direct stamen development. C-class AG alone governs carpel formation, and D-class genes contribute to ovule development within carpels, with E-class genes like SEPALLATA (SEP) proteins enabling all floral organ identities by forming higher-order complexes.^[30]^[31] Beyond floral structures, MADS-box genes regulate non-floral developmental processes, including root apical meristem maintenance and fruit ripening. In Arabidopsis, the AGAMOUS-like 12 (AGL12) gene, also known as XAL1, controls root meristem cell proliferation by influencing the transition from proliferative to elongation zones, with mutants exhibiting shortened roots due to reduced cell production rates. In tomato, the AGAMOUS-like 1 (TAGL1) gene integrates into the ripening regulatory network, where its downregulation delays climacteric fruit softening and alters pigmentation by repressing ethylene-independent pathways.^[32]^[33] These functions are mediated by intricate regulatory networks involving MADS-box protein complexes that form tetrameric quartets, which bind to multiple CArG-box DNA motifs to induce chromatin looping and activate target genes. In the floral quartet model, such tetramers—comprising two dimers of MIKC-type MADS proteins—stabilize DNA loops over short ranges (less than 300 bp), facilitating cooperative transcriptional activation essential for organ specification. Recent studies in rice highlight how OsMADS1 influences yield by modulating grain size through splicing variations that affect endosperm monosaccharide loading, thereby enhancing grain thickness and quality.^[30]^[34]^[35] A subset of MADS-box genes also responds to abiotic stresses during development, integrating environmental cues with growth processes. Genome-wide profiling in Camelina sativa identified 325 MADS-box genes, several of which exhibit differential expression under drought, with type II members upregulated in roots and shoots during organ development, suggesting roles in stress resilience while maintaining developmental progression.^[36]

Roles in Animals and Fungi

In animals, MADS-box genes primarily belong to the MEF2 and SRF subfamilies, which play crucial roles in developmental processes such as myogenesis and neurogenesis. The MEF2 subfamily, including MEF2A, regulates muscle differentiation and neuronal survival by activating transcription of genes involved in these pathways. For instance, MEF2A is essential for cardiac development, where it promotes myocardial differentiation and heart morphogenesis in vertebrates. Similarly, SRF, another key MADS-box transcription factor, mediates serum response pathways that drive cell proliferation and cytoskeletal organization through interactions with cofactors like MRTFs. SRF binds to CArG boxes in target gene promoters, facilitating expression of genes critical for immediate-early responses and smooth muscle differentiation. In fungi, MADS-box proteins such as MCM1 in Saccharomyces cerevisiae are involved in regulating mating processes. MCM1 controls the expression of mating-type-specific genes by forming complexes with alpha1 or alpha2 proteins, thereby directing cell-type identity and pheromone responses. Specifically, MCM1 activates alpha-specific genes in alpha cells and represses a-specific genes in cooperation with alpha2, enabling proper mating-type switching and pheromone signaling pathways essential for sexual reproduction. These functions highlight MCM1's role as a master regulator in yeast mating differentiation. Across kingdoms, MADS-box proteins exhibit conserved mechanisms of basic transcription activation via DNA binding and cofactor interactions, though animal and fungal orthologs form fewer and simpler complexes—typically dimers—compared to the elaborate quartets seen in plants. This conservation underscores their ancient eukaryotic origin, with the MADS domain enabling sequence-specific regulation of developmental genes. In humans, dysregulation of MADS-box genes like SRF contributes to diseases such as cardiac hypertrophy, where altered SRF activity leads to pathological remodeling and increased fetal gene expression in the heart. Mutations or overexpression of SRF have been linked to hypertrophic cardiomyopathy in model systems, emphasizing its role in maladaptive cardiac responses. Research on these implications has largely stabilized since the 2010s, with foundational insights from earlier studies guiding current understanding.

Evolutionary and Applied Aspects

Evolutionary Origins

MADS-box genes trace their origins to an ancient duplication event in the last eukaryotic common ancestor, estimated to have occurred over a billion years ago, prior to the divergence of plants and animals. This duplication gave rise to the two primary lineages: Type I (SRF-like) and Type II (MEF2-like), which are present in both opisthokonts (animals and fungi) and plants. In opisthokonts, Type I genes resemble SRF transcription factors involved in serum response, while Type II genes are akin to MEF2 factors regulating muscle development in early metazoans. Phylogenetic analyses of sequences from diverse eukaryotes, including yeast and nematodes, support this deep homology, indicating that the core MADS domain was already functional in regulating gene expression in the eukaryotic stem lineage.^[2]^[37] Type I (SRF-like) genes represent one basal lineage, while Type II (MEF2-like) genes represent the other, with forms evident in early metazoan evolution and preserved across opisthokonts. These genes likely facilitated foundational roles in cellular signaling and differentiation before the radiation of multicellular life. In plants, Type I genes exhibit a polyphyletic origin, with some homologs aligning closely to opisthokont SRF-type genes, suggesting independent retention and adaptation in green lineages. Evidence from basal opisthokont relatives, such as Capsaspora owczarzaki, underscores the antiquity of these motifs, as genomic surveys reveal conserved MADS-domain elements in pre-metazoan unicellular forms.^[2]^[37]^[38] In plants, significant expansions of MADS-box genes occurred during streptophyte evolution, particularly through duplications of Type II genes in the stem lineage of charophytes and land plants. Two ancient duplication events in the streptophyte ancestor generated the MIKC^C and MIKC^* subfamilies of Type II genes, enabling diversification into regulatory roles specific to plant development. Further proliferation in angiosperms was driven by whole-genome duplications, which increased gene copy numbers across major clades; for instance, analyses of Arabidopsis and other eudicots reveal 12 principal MIKC^C clades, many arising from duplications around 200-300 million years ago, with paralogs retained in modern flowering plants. These events laid the foundation for specialized functions in floral organ identity and seed development.^[37]^[39] Diversification of MADS-box genes post-duplication was primarily propelled by neofunctionalization, where duplicated copies acquired novel expression patterns and protein interactions, adapting to lineage-specific selective pressures. In angiosperms, this process is evident in the divergence between monocots and eudicots, where whole-genome triplications in eudicots (e.g., ~117 million years ago) and duplications in monocots led to distinct clade expansions; for example, eudicot-specific paralogs like those in the SEPALLATA clade show altered roles in fruit morphogenesis, while monocot counterparts emphasize vegetative transitions. Recent genomic comparisons highlight how structural variations, such as C-terminal modifications, facilitated these shifts, with neofunctionalized genes contributing to the morphological diversity of flowers and fruits across angiosperm clades.^[37]^[40]^[39]

Applications in Biotechnology

MADS-box genes have been engineered in various crops to modify flowering time, enabling adaptations to environmental conditions and potentially increasing yield through optimized growth cycles. For instance, overexpression of the floral MADS-box gene OsMADS45 in rice activates downstream florigen genes like Hd3a and RFT1 while suppressing Hd1, reducing days to heading by approximately 40 days, though it results in reduced plant height, panicle fertility, and grain yield.^[41] Similarly, overexpression of the SOC1-like MADS-box gene ZmSOC1 in maize promotes tasseling and silking by 3–5 days, shortens plant height by approximately 10–15 cm, and maintains or enhances grain yield (e.g., no reduction in grain dry weight per plant at high densities), demonstrating potential for yield boost in FT-like pathways.^[42] CRISPR/Cas9-mediated editing of Type II MADS-box genes has improved drought tolerance in crops by targeting stress-responsive regulators. In rice, knockout of the Type II gene OsMADS26 enhances drought resistance by upregulating downstream genes involved in osmotic adjustment and antioxidant defense, resulting in 30–50% higher survival rates under severe water deficit compared to wild-type plants.^[43] For Camelina sativa, a promising oilseed crop, expression profiling reveals that several Type II MADS-box genes (e.g., CsMADS265) are upregulated up to 35-fold during drought stress, suggesting targets for CRISPR editing to enhance resilience.^[36] These approaches leverage the regulatory roles of MADS-box proteins in developmental pathways to confer abiotic stress tolerance. In synthetic biology, MADS-box genes serve as components for designing sensors that monitor plant developmental stages, with applications in biofuel crops like sugarcane and Brachypodium distachyon. For example, in sugarcane, expression profiling has identified numerous MADS-box genes differentially expressed during stem ripening, correlating with sucrose accumulation and suggesting roles in biomass-related processes.^[44] Such sensors could activate downstream pathways for enhanced lignocellulosic biomass without altering core functions. Despite these advances, challenges persist in applying MADS-box engineering, particularly off-target effects in polyploid genomes where multiple homeologs complicate precise editing, leading to unintended pleiotropic impacts on development in crops like wheat.^[45] Ethical considerations in GMO deployment include equitable access to improved varieties and long-term ecological risks from altered gene flow, necessitating rigorous regulatory frameworks.^[46]

References

[1]
A hitchhiker's guide to the MADS world of plants | Genome Biology
Jun 28, 2010 · The MADS-box genes, encoding the MADS-domain family of transcription factors, are involved in controlling all major aspects of the life of land ...
[2]
An ancestral MADS-box gene duplication occurred before ... - PNAS
MADS-box genes encode transcriptional regulators of diverse and important biological functions. In plants, MADS-box genes regulate flower, fruit, leaf, and ...
[3]
https://www.sciencedirect.com/science/article/pii/S0378111904007620
[4]
MADS-Box Genes Are Key Components of Genetic Regulatory ...
MADS-box genes are key components of gene regulatory networks involved in plant responses to stress and plant developmental plasticity.
[5]
The protein encoded by the Arabidopsis homeotic gene agamous ...
Jul 5, 1990 · The agamous gene therefore probably encodes a transcription factor that regulates genes determining stamen and carpel development in wild-type flowers.
[6]
MADS-box gene family in rice - BMC Genomics
Jul 18, 2007 · The MADS-box family members, identified initially as floral homeotic genes, are one of the most extensively studied transcription factor genes ...
[7]
Regulatory mechanisms of MADS-box transcription factors in growth ...
This article reviews the molecular mechanisms and genetic networks by which MADS-box transcription factors influence rice yield.
[8]
Structure of serum response factor core bound to DNA - Nature
Aug 10, 1995 · The human serum response factor is a transcription factor belonging to the MADS domain protein family with members characterized from the plant ...
[9]
Genome-Wide Analysis of the MADS-Box Gene Family in Maize - NIH
Dec 7, 2021 · ... Mα, Mβ, Mγ, and Mδ. Gene duplications are regarded as products of ... Developmental and evolutionary diversity of plant MADS-domain factors: ...3. Results · 3.3. Gene Structure And... · Figure 2
[10]
Structural Basis for Plant MADS Transcription Factor Oligomerization
The crystal structure of the K domain from SEP3 was the first and only structurally characterized oligomerization module of a plant MADS TFs available to date ( ...
[11]
Structural basis for the oligomerization of the MADS domain ...
Structural basis for the oligomerization of the MADS domain ... Epub 2014 Sep 16. Authors. Sriharsha Puranik , Samira Acajjaoui ...Missing: box | Show results with:box
[12]
Structure and Evolution of Plant MADS Domain Transcription Factors
The approximate 174 nucleotide residues of DNA encoding the MADS domain have been termed the MADS box; therefore, the genes they are part of are known as MADS ...Missing: coin | Show results with:coin
[13]
Structural Basis for Plant MADS Transcription Factor Oligomerization
Jun 14, 2019 · In this mini review, we describe the overall structure of the type II “MIKC” type MADS TFs in plants, with a focus on the K domain, a critical oligomerization ...
[14]
OsMADS23 phosphorylated by SAPK9 confers drought and salt ...
Aug 3, 2021 · More importantly, the SnRK2-type protein kinase SAPK9 physically interacts with and phosphorylates OsMADS23, and increase its stability and ...
[15]
Molecular and Phylogenetic Analyses of the Complete MADS-Box ...
Analysis of the complete Arabidopsis genome sequence revealed 107 genes encoding MADS-box proteins, of which 84% are of unknown function. Here, we provide a ...
[16]
The Emerging Importance of Type I MADS Box Transcription Factors ...
The acronym MADS (Schwarz-Sommer et al., 1990) is derived from the initials of MINICHROMOSOME MAINTENANCE1 (MCM1, Saccharomyces cerevisiae; Passmore et al ...Missing: coin | Show results with:coin
[17]
Antiquity and Evolution of the MADS-Box Gene Family Controlling ...
As mentioned above, we selected 24 representative sequences of 142 amino acid sites without gaps, including the MADS-domain, the K-domain, and the conserved ...
[18]
Updated Phylogeny and Protein Structure Predictions Revise the ...
Aug 31, 2023 · Varying by domain architecture, MADS-box TFs in land plants are categorized into Type I (M-type) and Type II (MIKC-type). Type I and II genes ...<|control11|><|separator|>
[19]
MIKC-type MADS-box transcription factor gene family in peanut
In addition, we found that the number of peanut MIKC-type MADS-box genes (93) was greater than that in Arabidopsis (44) (Parenicová et al., 2003), Medicago ...Abstract · Introduction · Results · Discussion
[20]
Transcript Profiling of MIKCc MADS-Box Genes Reveals Conserved ...
Aug 31, 2021 · In plants, Type II MADS-box genes are called MIKC type, including MIKCc and MIKC* sub-types, an acronym of the four different domains that have ...
[21]
Ancestral duplication of MADS‐box genes in land plants ...
Aug 16, 2024 · Specifically, in land plants, the MADS-box gene family has undergone substantial expansion and provided the genetic raw material for many ...
[22]
Genome-wide analysis of MADS-box transcription factor gene family ...
Mar 7, 2024 · The number of MIKC-type MADS-box genes per subgenome in wild emmer is 93/2 = 46.5 compared to that of rice (43) and Arabidopsis (45) Type II ...
[23]
Identification of MADS-box genes in flax and their role in stress
Dec 20, 2024 · MADS is an acronym formed from the family's founding members: the yeast MCMI gene; the Arabidopsis AGAMOUS gene; the snapdragon DEFICIENS gene; ...Missing: coined | Show results with:coined
[24]
MADS-domain transcription factors and the floral quartet model of ...
Sep 15, 2016 · The acronym 'MADS' refers to the four founder genes MINICHROMOSOME MAINTENANCE FACTOR1 (MCM1; from Saccharomyces cerevisiae), AGAMOUS (AG; from ...
[25]
Reflections on the ABC model of flower development - PMC
Thus, the MADS-box genes are necessary for the specification of floral organ identity and are sufficient (along with E-class) to convert leaves into floral ...
[26]
AGAMOUS-Related MADS-Box Gene, XAL1 (AGL12), Regulates ...
Abstract. MADS-box genes are key components of the networks that control the transition to flowering and flower development, but their role in vegetative d.
[27]
TOMATO AGAMOUS‐LIKE 1 is a component of the fruit ripening ...
Dec 8, 2009 · In this study, we have identified the TOMATO AGAMOUS-LIKE 1 MADS box protein as an additional element of the regulatory network that mediates ...
[28]
MADS Domain Transcription Factors Mediate Short-Range DNA ...
Studies demonstrated that MADS domain protein complexes often interact with DNA by contacting multiple nearby CArG box sequences, separated by less than 300 bp ...
[29]
Genome-wide characterization and expression profiling of MADS ...
Mar 18, 2025 · These genes were systematically categorized into two principal groups: type I (comprising Mα, Mβ, and Mγ) and type II (including MI-KCC and MIKC ...
[30]
The origin, evolution, and diversification of MADS-box transcription ...
Sep 8, 2025 · Our analyses suggested that plant type I genes have a polyphyletic origin and that the two subfamilies of plant type II genes (i.e., MIKCC and ...
[31]
Unexpected repertoire of metazoan transcription factors in the ...
Myocite enhancer factors 2 (Mef2) are the metazoan representatives of Type II MADS-box genes. They are characterized by the presence of a Mef2 domain following ...
[32]
The major clades of MADS-box genes and their role in the ...
MADS-box genes encode a family of transcription factors which control diverse developmental processes in flowering plants ranging from root to flower and fruit ...
[33]
Gene Duplication and Functional Diversification of MADS-Box ... - NIH
Aug 17, 2024 · The evolution of the MADS-box gene family is essential for the rapid differentiation of floral organs and fruit types in angiosperms.
[34]
Ectopic expression of OsMADS45 activates the upstream genes ...
OsMADS14 overexpression significantly reduces flowering time and causes flower bud development as early as transgenic rice cultured in regeneration medium (Jeon ...
[35]
Utilizing MIKC-type MADS-box protein SOC1 for yield potential ...
Jun 6, 2021 · Overexpression of Zea mays SOC gene promotes flowering, reduces plant height, and leads to no reduction in grain production per plant, suggesting enhanced ...<|separator|>
[36]
MADS-box genes are involved in abiotic stress response in different...
MADS-box genes are involved in abiotic stress response in different developmental processes in several plants. (A) OsMADS26 is a negative regulator of drought ...
[37]
MADS-box Gene Family Role in S. spontaneum Development
Nov 25, 2020 · We identified 182 MADS-box sequences in the S. spontaneum genome, which were annotated into 63 genes, including 6 (9.5%) genes with four alleles.Missing: paper | Show results with:paper
[38]
Broadening the GMO risk assessment in the EU for genome editing ...
Aug 11, 2020 · Challenges to plant breeding include polyploidy, a large number of orthologous genes, heterozygosity, repetitive DNA and linkage drag. Major ...
[39]
What are the Ethical Concerns of Genome Editing?
Aug 3, 2017 · Most ethical discussions about genome editing center on human germline editing because changes are passed down to future generations.