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Morelet's tree frog

Morelet's tree frog (Agalychnis moreletii), also known as the black-eyed leaf frog, is a species of arboreal frog in the subfamily Phyllomedusinae (family Hylidae), native to the humid lowland and montane forests of Central America, ranging from eastern Mexico through Belize, Guatemala, El Salvador, and Honduras.^[1] Adults exhibit a bright green dorsal coloration with orange flanks and webbing, pale yellow or white ventral surfaces, and distinctive deep purple to red irises marked by black reticulations, aiding in camouflage among foliage; males typically measure 60-66 mm in snout-vent length (SVL), while females reach up to 83 mm SVL.^[1]^[2] The species inhabits elevations from 650 to 1,300 meters, often near ponds or streams in disturbed as well as primary forests, and is nocturnal, with males producing advertisement calls resembling "wor-or-orp" to attract mates during breeding seasons aligned with rainfall.^[1] Reproduction involves axillary amplexus, with females depositing clutches of 50-75 pigmented eggs on leaves or rocks overhanging water, which hatch after 9-12 days, allowing tadpoles to drop into the water below to complete metamorphosis.^[1] Although historically threatened by chytridiomycosis, habitat destruction, and collection for the pet trade—leading to a critically endangered status until 2016—recent assessments indicate stable or recovering populations, resulting in its current IUCN classification as least concern, supplemented by CITES Appendix II protections.^[1]^[3]

Taxonomy and etymology

Scientific classification

Morelet's tree frog (Agalychnis moreletii (Duméril, 1853)) is classified within the order Anura of the class Amphibia.^[1]^[4]

Taxon	Classification
Kingdom	Animalia
Phylum	Chordata
Class	Amphibia
Order	Anura
Family	Hylidae
Subfamily	Phyllomedusinae
Genus	Agalychnis
Species	A. moreletii

This placement reflects its membership among neotropical tree frogs characterized by adaptations for arboreal life, such as fully webbed feet.^[1]^[4] The binomial name derives from the original description by André Marie Constant Duméril in 1853, initially under Hyla moreletii.^[1]

Naming and discovery

The Morelet's tree frog (Agalychnis moreletii) was first scientifically described in 1853 by French herpetologist André Marie Constant Duméril, who placed it in the genus Hyla as Hyla moreletii.^[5] The description was based on syntypes collected during expeditions in Central America.^[1] The species' syntypes were gathered by French naturalist and illustrator Pierre Marie Arthur Morelet (1807–1873), who explored regions including Mexico and Guatemala in the mid-19th century.^[1] The specific epithet moreletii directly honors Morelet for supplying these original specimens to Duméril, reflecting standard taxonomic practice of the era in recognizing collectors' contributions to herpetological science.^[1] Subsequent taxonomic revisions reclassified the species into the genus Agalychnis within the subfamily Phyllomedusinae, aligning it with other Neotropical leaf-breeding tree frogs based on morphological and phylogenetic evidence.^[5]

Physical description

Morphology and coloration

Morelet's tree frog (Agalychnis moreletii) is a medium-sized arboreal hylid with adult males attaining snout-vent lengths (SVL) of 60.5–64.2 mm and females typically larger at 55–75 mm SVL.^[1] The body form is slender, adapted for life in the canopy, with a rounded snout in dorsal view, obtuse in profile, laterally directed nostrils, and concave loreal region.^[1] The skin is smooth to weakly granular dorsally and coarsely areolate ventrally, with granulation around the vent.^[1] Limbs feature extensively webbed fingers (relative lengths I < II < IV < III) and toes, both bearing broadly expanded adhesive discs—toe discs slightly smaller than finger discs—facilitating climbing, parachuting, and gliding between trees.^[1]^[3] Males have paired vocal slits, a single subgular vocal sac, and small nuptial excrescences on the prepollex during breeding; the pupil is vertically elliptical.^[1] Dorsal coloration varies from dark to light green for foliage camouflage, with orange on flanks, inner limb surfaces, and webbing.^[1] Ventral surfaces are light yellow or white, while the iris is dark red to deep purple—often appearing blackish, contributing to the "black-eyed" moniker—and the palpebral membrane shows gold reticulations.^[1] Individuals can subtly alter green shades to match surroundings.^[6]

Sexual dimorphism

Adult females of Agalychnis moreletii are significantly larger than males, with mean snout-vent length (SVL) of approximately 77 mm and mass of 21.6 g for females compared to 61.8 mm SVL and 7.9 g for males.^[3] This size disparity aligns with patterns observed across many hylid frogs, where larger female body size facilitates greater reproductive output through increased egg production.^[2] Males exhibit distinct reproductive morphology, including paired vocal slits and a single subgular vocal sac used for advertisement calling during breeding.^[1] Breeding males develop dark nuptial pads on the thumbs (prepollex), which aid in grasping females during amplexus, and possess a more truncate snout shape relative to the broader snout in females.^[3] ^[1] Coloration shows no pronounced sexual differences, with both sexes featuring vibrant lime green dorsal surfaces, yellow ventral areas, and striking black eyes.^[6]

Distribution and habitat

Geographic range

Agalychnis moreletii inhabits disjunct populations across southeastern Mexico, Belize, Guatemala, El Salvador, and Honduras.^[1]^[5] In Mexico, records extend from northeastern Puebla and southeastern Veracruz southward through Oaxaca and Chiapas.^[7]^[1] The distribution features two primary ranges: one along the Pacific versant and another on the Atlantic slope, reflecting habitat fragmentation in montane and lowland regions.^[1] Populations in Honduras have been documented across multiple departments, indicating broader occupancy within that country.^[5] The species' range does not extend into Nicaragua or further south, confining it to these northern Central American locales.^[8]^[6]

Environmental requirements

Morelet's tree frog inhabits lowland to montane moist forests on mountain slopes, occurring in both pristine and secondary/disturbed areas.^[1]^[9] These environments feature dense vegetation, including trees and overhanging foliage where the species perches arboreally at heights of 6–10 meters.^[3] Proximity to standing or slow-moving water bodies, such as streams, ponds, and lakes, is essential, particularly for breeding, as females deposit egg clutches on vegetation or rocks suspended above these sites to allow tadpoles to drop into the water upon hatching.^[1]^[6] The species occupies elevations from 200 to 1,950 meters above sea level, with a core range of 600–1,300 meters where conditions align with its physiological tolerances.^[3]^[1] Its distribution corresponds to Köppen climate zones of tropical and mid-latitude temperate types, characterized by high humidity and seasonal precipitation patterns that maintain forest moisture.^[3] Mean air temperatures range from 16–22.5°C across seasons, with daytime maxima up to 25°C and nighttime lows around 14–17°C; the rainy season (June–October) brings 200–530 mm of precipitation, supporting breeding, while the drier period (November–May) sees 30–160 mm, during which the frog relies on residual humidity from vegetation and microhabitats.^[3] Tolerance for moderate habitat disturbance indicates adaptability to edge effects, but sustained moist conditions are critical to prevent desiccation, with the species absorbing water directly from leaf surfaces in humid arboreal niches.^[1]^[3] Water quality in breeding ponds influences larval survival, with preferences for sites minimizing predation and desiccation risks while providing adequate oxygenation.^[2]

Behavior and life history

Daily and seasonal activity

Morelet's tree frog (Agalychnis moreletii) exhibits strictly nocturnal activity patterns, foraging for insects and engaging in vocalizations primarily after dusk, while remaining inactive and hidden during daylight hours to minimize predation risk and water loss in its humid tropical habitat.^[1] ^[6] Males produce chorus calls consisting of a single "wor-or-orp" note at intervals of one to two minutes, with a dominant frequency around 1.5 kHz, typically from elevated perches near water bodies during nighttime hours.^[1] Seasonally, activity intensifies at the onset of the rainy season, when males descend from the forest canopy to aggregate in choruses around ponds and temporary water bodies for breeding, triggered by heavy precipitation that creates suitable larval habitats.^[1] ^[6] Females arrive later following sustained rains, leading to an extended reproductive period aligned with wetter months (typically May to October in its Central American range), after which populations retreat to higher canopy elevations during drier intervals with reduced surface activity.^[1]

Diet and predation

Morelet's tree frogs (Agalychnis moreletii) are insectivorous, foraging nocturnally for arthropods including flies, moths, ants, and other small invertebrates captured via adhesive tongue projection from arboreal perches.^[10] Adults in captivity consume prey items up to 3/4 inch in length, consistent with wild feeding on mobile insects suited to their rapid tongue kinematics observed in phyllomedusine frogs.^[11] ^[12] Predators of A. moreletii include arboreal snakes such as the cat-eyed snake (Leptodeira septentrionalis), which target clutches and adults in forested habitats.^[13] Embryos respond to mechanical disturbance from snake attacks by hatching prematurely, typically around 7 days post-oviposition at Gosner stage 23, to escape predation.^[1] Defensive adaptations include body inflation to increase apparent size when threatened and reliance on skin secretions containing irritant toxins, advertised by their bright green coloration, which deter potential predators.^[10] These mechanisms, combined with diurnal camouflage among foliage, reduce vulnerability during inactive periods.^[10]

Reproduction and development

Males of Agalychnis moreletii aggregate in choruses around ponds following heavy rains, producing advertisement calls characterized by a "wor-or-orp" sound at frequencies of 1,100–1,260 Hz, with calls emitted at intervals of 1–2 minutes.^[1] Females approach calling males, leading to axillary amplexus, in which larger males exhibit a mating advantage, particularly during drier years when competition is intensified.^[1] In amplexus, females deposit gelatinous clutches of 50–75 eggs, averaging 71.5 ± 4.8 eggs per clutch (n=20), on vegetation or rocks overhanging permanent or semi-permanent water bodies.^[1]^[3] The eggs possess a slight green tint and demonstrate high fertilization success, with 100% fertilization observed in 45 of 46 clutches.^[1] Embryos typically hatch 9–12 days after oviposition, primarily at night over a 2–3 day period, though hatching can occur prematurely after approximately 7 days (Gosner stage 23) in response to environmental disturbances such as submersion or predation risks, reflecting adaptive developmental plasticity.^[1] Hatched tadpoles fall into the water below and feature a robust body deeper than broad, with an acuminate tail roughly twice the body length and well-developed musculature; they possess a ventrolateral sinistral spiracle, a vent tube positioned to the right of the caudal fin, and purplish-brown coloration with two upper and three lower rows of denticles.^[1] Tadpoles develop aquatically in ponds, exhibiting rapid growth modulated by factors including temperature and desiccation, which influence morphology, body condition, metamorphosis timing, and synchrony; higher temperatures and desiccation stress accelerate growth rates but reduce size at metamorphosis.^[14] Freshly metamorphosed froglets initially display uniform grey or brown dorsal coloration, shifting to light green within a few days.^[3]

Ecology and interactions

Symbiotic relationships

Morelet's tree frog (Agalychnis moreletii) maintains mutualistic symbiotic relationships with cutaneous bacterial communities on its skin, which contribute to defense against pathogenic fungi such as Batrachochytrium dendrobatidis (Bd), the causative agent of chytridiomycosis.^[15] These bacteria, including isolates from genera like Janthinobacterium and Pseudomonas, produce antifungal metabolites that inhibit Bd growth, enhancing host survival in environments where the pathogen is prevalent.^[16] Studies on captive individuals demonstrate that disruptions to this microbiome, such as from passive integrated transponder (PIT) tagging, reduce bacterial diversity and allow opportunistic fungi to proliferate, underscoring the symbiosis's protective role.^[15] Bacterial strains isolated from wild A. moreletii and closely related Agalychnis callidryas exhibit variable antifungal efficacy against Bd's global panzootic lineage, with not all symbionts providing universal inhibition; this variability suggests context-dependent mutualism influenced by environmental factors like temperature and humidity.^[16] The frog's skin provides nutrients and habitat for these microbes, which in turn bolster innate immunity, a dynamic observed across amphibian species but critical for arboreal hylids like A. moreletii facing epizootic threats.^[15] No evidence indicates obligate dependence, but empirical data from culturable isolates confirm the symbiosis's contribution to disease resistance without altering baseline physiological traits like corticosterone levels.^[17] Parasitic interactions, potentially including helminths or protozoans, remain undescribed in detail for A. moreletii, though general amphibian ecology implies incidental ectoparasites or endoparasites that exploit the frog as a host without mutual benefit. Commensal relationships with phytotelmata-dwelling invertebrates in breeding sites are inferred but lack species-specific documentation beyond habitat overlap.^[1]

Role in ecosystem

Morelet's tree frog (Agalychnis moreletii) occupies a mid-level trophic position in neotropical moist forest ecosystems, primarily functioning as a nocturnal predator of small arthropods, including insects such as crickets, flies, and roaches, which it captures from foliage in the canopy and understory.^[3]^[18] This feeding behavior contributes to the regulation of invertebrate populations, with neotropical amphibians like tree frogs demonstrated to reduce aerial insect abundance by approximately 28% and associated plant herbivory by up to 80% on local scales.^[19] As prey, adults support higher trophic levels, falling victim to arboreal vertebrates such as snakes and birds, while eggs face predation from wasps and other invertebrates, prompting adaptive premature hatching in embryos upon detection of threats like snake activity. Tadpoles, upon dropping into breeding ponds, consume algae and detritus, facilitating nutrient cycling and decomposition in aquatic microhabitats, thereby linking terrestrial and freshwater food webs. These interactions underscore the frog's role in maintaining biodiversity and energy flow within its habitat, though specific quantitative impacts remain understudied for this species.^[20]

Conservation and threats

Population status and trends

Morelet's tree frog (Agalychnis moreletii) is currently assessed as Least Concern on the IUCN Red List, reflecting a downlisting from Critically Endangered status held between 2004 and 2016.^[3] This reassessment in 2017 was based on evidence of a wider distribution than previously documented and tolerance for some habitat modification, reducing the inferred risk of extinction.^[21] Despite the improved global status, no comprehensive population estimates exist, as field surveys for arboreal amphibians remain challenging and sporadic. Populations have experienced severe historical declines, particularly in Mexico, where they have nearly disappeared due to chytridiomycosis caused by the pathogen Batrachochytrium dendrobatidis.^[1] In Guatemala and Honduras, ongoing reductions are attributed to habitat destruction from small-scale agriculture, though the species persists in modified lowland and montane forests.^[1] Earlier projections from the early 2000s anticipated over 80% population loss within a decade due to disease and habitat loss combined, but recent monitoring in some Central American regions indicates stability without rapid collapse from chytrid infection.^[22] Current trends suggest localized persistence rather than uniform recovery, with the species listed under CITES Appendix II to regulate international trade amid historical collection pressures.^[21] Continued monitoring is recommended, as fragmented habitats and emerging disease threats could reverse gains in vulnerable subpopulations.^[1]

Primary threats

The primary threats to Agalychnis moreletii include habitat destruction driven by agricultural expansion and urbanization, as well as the fungal disease chytridiomycosis caused by Batrachochytrium dendrobatidis. Deforestation for lowland montane forest conversion to farmland and settlements has fragmented populations across its range from Mexico to northern Costa Rica, reducing available breeding sites in humid forest canopies.^[1] Illegal activities such as mining and cultivation of illicit crops exacerbate habitat loss by introducing soil erosion and chemical runoff into aquatic breeding habitats.^[23] Chytridiomycosis has caused severe population declines, particularly in Mexico where local populations have nearly vanished due to the pathogen's disruption of skin function, leading to electrolyte imbalances and cardiac arrest in infected individuals.^[1] The fungus thrives in the moist environments preferred by the species, with infection rates historically high enough to drive extirpations in northern portions of the range.^[6] Pollution from agricultural pesticides and mining effluents further compounds vulnerability by contaminating oviposition sites and larval habitats, impairing development and increasing susceptibility to pathogens.^[23] Overcollection for the pet trade, though less quantified, contributes to localized depletions, as the species' striking appearance attracts international demand despite CITES Appendix II protections implemented since 1987.^[24] Climate variability may indirectly intensify threats by altering rainfall patterns essential for reproduction, though empirical data on direct impacts remain limited.^[1]

Human impacts and management

Human activities have significantly impacted Agalychnis moreletii populations through habitat destruction, primarily driven by subsistence and smallholder agriculture, which fragments lowland moist forests in Central America.^[6] Deforestation for these purposes has reduced available breeding sites, as the species relies on intact riparian vegetation for oviposition on leaves overhanging water bodies.^[2] Pollution from agricultural runoff and illegal mining further degrades water quality essential for tadpole development, while overcollection for the international pet trade—evidenced by U.S. imports of over 221,960 Agalychnis specimens between approximately 2000 and 2010—exacerbates local declines.^[1] These pressures, compounded by indirect facilitation of chytridiomycosis spread through habitat alteration and trade, prompted the species' classification as Critically Endangered by the IUCN from 2004 to 2016.^[23] Conservation management has focused on habitat protection and ex-situ efforts to mitigate these impacts. Reassessment in 2017 led to an IUCN downlisting to Least Concern, reflecting expanded surveys revealing wider distribution and some population stability, alongside targeted interventions like forest reserve establishment in Guatemala's Sierra Caral to preserve contiguous habitat remnants.^[23] ^[25] Captive breeding programs, guided by European Association of Zoos and Aquaria (EAZA) protocols, emphasize optimizing enclosure conditions to support reproduction and symbiotic bacterial communities that enhance fitness, with releases aimed at bolstering wild stocks.^[3] Community-based initiatives in Honduras and Guatemala promote alternative livelihoods to reduce agricultural encroachment and include monitoring for chytridiomycosis to inform disease management, though sustained funding and enforcement against illegal activities remain critical for long-term viability.^[26]

References

[1]
Agalychnis moreletii - AmphibiaWeb
Agalychnis moreletii is critically endangered. Populations in Mexico have almost entirely disappeared, likely due to chytridiomycosis (Lips et al. 2004; Frías- ...
[2]
Oviposition Site Selection and Conservation Insights of Two Tree ...
Jul 6, 2020 · The black-eyed tree frog (Agalychnis moreletii Duméril, 1853) is a critically endangered species (IUCN, 2017) that ranges from Mexico to ...
[3]
[PDF] EAZA Amphibian TAG Best Practice Guidelines for the black-eyed ...
The Black-eyed tree frog (Agalychnis moreletii) was assessed as Critically Endangered by the IUCN from 2004 to 2016. It was reclassified as Least Concern in ...Missing: Morelet's | Show results with:Morelet's
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Agalychnis moreletii - NCBI - NLM
Black-eyed leaf frog (Agalychnis moreletii) is a species in the family Hylidae (tree frogs) ... Current scientific name: Agalychnis moreletii. (Dumeril ...
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Agalychnis moreletii (Duméril, 1853) - Amphibian Species of the World
Rev., 38: 37, detailed the departmental distribution in Honduras. See photograph, map, description of geographic range and habitat, and conservation status ...
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Black-eyed Leaf Frog (Agalychnis moreletii) - World Land Trust
Scientific Name: Agalychnis moreletii. IUCN Red List status: Critically Endangered. IUCN Species Range Map. Description. Females (6.5-8.5cm) of this species are ...
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(PDF) Comments on the distribution and conservation of Morelet's ...
Agalychnis moreletti (Dumeril, 1853) is distributed in disjunct populations on the Atlantic versant from northeastern. Puebla and southeastern Veracruz, ...<|separator|>
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Morelet's tree frog - Facts, Diet, Habitat & Pictures on Animalia.bio
### Summary of Diet, Feeding, Nutrition, Predation, Predators, and Defense Mechanisms for Morelet's Tree Frog (Agalychnis moreletii)
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Listed as Critically Endangered because of a projected population decline, estimated to be more than 80% over the next ten years, probably due to ...Missing: 2023 | Show results with:2023<|separator|>
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Black-eyed leaf frog facts, distribution & population - BioDB
Morelet's tree frog. Popeye hyla. Physical traits. Length: 2 – 3 in (5 – 8 cm) Lifespan: 5 – 10 years Eggs: 50 – 75 eggs. Habitats: Urban Forest Fresh water ...
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Black-Eyed Tree Frog Agalychnis Moreletii (Captive Bred)
Size: captive bred Black-Eyed tree frogs between 8-10 weeks will measure about 1 inch & will quickly grow to 2 inches within 3-4 months. After about 10 months ...
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The rapid tongue protraction, extended gape cycle and fly-swatter action may have evolved in response to a diet of large, rapidly moving insects.Missing: Phyllomedusidae | Show results with:Phyllomedusidae
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Cat-eyed snake fails to catch tree frog in Belize - Facebook
Jan 11, 2018 · Predator: cat-eyed snake (Leptodeira septentrionalis) Prey: Morelet's tree frog (Agalychnis moreletii) Location: Belize rain forest Photo: ...
[14]
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Mar 17, 2020 · Hypothesis: Temperature and desiccation will increase growth rates in Agalychnis moreletii tadpoles, reducing size at metamorphosis and other ...
[15]
Tagging Frogs with Passive Integrated Transponders Causes ...
Symbiotic bacterial communities play a key role in protecting amphibians from infectious diseases including chytridiomycosis, caused by the pathogenic ...
[16]
Amphibian Symbiotic Bacteria Do Not Show a Universal Ability To ...
May 5, 2015 · Fifty-six bacterial strains isolated from wild Agalychnis callidryas and Agalychnis moreletii frogs were initially screened for their antifungal ...
[17]
Effects of visible implanted elastomer marking on physiological traits ...
Oct 3, 2014 · We show that marking of red-eyed tree frogs (Agalychnis callidryas) with visible elastomer has no effect on adrenal response.
[18]
[PDF] Basic Information and Husbandry Guidelines for Agalychnis lemur ...
Scientific name: Agalychnis lemur (Boulenger, 1882). Vernacular names: Lemur Leaf Frog, Lemur Leaf Frog (German). Snout-vent length: males usually 30–38 mm, ...
[19]
[PDF] Ecological functions of neotropical amphibians and reptiles: a review
Energy flow through trophic chains: predators and prey: Herps as predators. Herps can regulate populations of certain organisms through biotic interactions ...
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Aug 13, 2013 · Amphibians and reptiles contributed directly and indirectly to the four types of ecosystem services: provisioning, regulating, cultural, and supporting.
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The species is found in Central America, from Mexico (Puebla and Veracruz) to north-western Honduras and central El Salvador. Lives in tropical moist lowland ...Missing: distribution | Show results with:distribution
[22]
Prevalence of Batrachochytrium dendrobatidis in Agalychnis ...
Between May and July 2008, we investigated a fungal pathogen in the critically endangered Morelet's treefrog (Agalychnis moreletii) at sites in El Salvador.Missing: ecosystem | Show results with:ecosystem
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Dec 20, 2017 · ... Agalychnis moreletii, in the Cuchumatanes of Guatemala. Photo by ... listed as threatened on the IUCN Red List. In general, species ...
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The genus Agalychnis consists of five species, all included in this proposal, with a distribution that includes ten range States from Mexico in the north to ...
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There are three main project objectives. First, the protection of the largest contiguous forest remnant in Sierra Caral for the conservation of endemic and ...Missing: deforestation | Show results with:deforestation
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Assessing threats and opportunities for Agalychnis moreletii conservation, linking reproductive success, habitat suitability and chytridiomycosis presence ...Missing: human | Show results with:human<|separator|>