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Pronucleus

A pronucleus is the haploid nucleus derived from either the sperm or egg gamete following fertilization, prior to the fusion of their genetic material to form the diploid zygote nucleus.^[1] In this stage, the male pronucleus forms when the sperm's highly condensed nucleus decondenses after entering the egg cytoplasm, while the female pronucleus arises from the egg's nucleus after completion of meiosis II.^[1] Each pronucleus contains 23 chromosomes and is enclosed by a nuclear envelope, representing a critical intermediate in the restoration of diploidy during reproduction.^[2] The formation of pronuclei is triggered by specific biochemical changes post-fertilization. In sea urchins, the sperm chromatin decondenses through the phosphorylation of sperm-specific histones.^[1] In mammals, decondensation is facilitated by factors from the egg such as glutathione, which reduces disulfide bonds in protamines, followed by the replacement of protamines with histones from the oocyte, allowing the male pronucleus to expand and mature over several hours.^[1] The female pronucleus, meanwhile, forms as the oocyte extrudes the second polar body, ensuring its haploid state.^[2] These transformations prepare the genetic material for syngamy, the union of paternal and maternal genomes.^[3] Pronuclear migration involves cytoskeletal dynamics that bring the two nuclei into close proximity. In sea urchins, the sperm centriole organizes microtubules into an aster that contacts and pulls the female pronucleus toward the male, with migration completing in under an hour.^[1] Mammalian zygotes exhibit a slower process, lasting about 12 hours, driven by astral microtubules from the centrosome and actin-based movements, during which DNA replication often begins within the pronuclei.^[1] This migration ensures proper alignment for genetic fusion and is essential for embryonic development; disruptions can lead to polyspermy or developmental arrest.^[3] The fusion of pronuclei, or syngamy, culminates in the formation of the zygote. In some invertebrates like sea urchins, the pronuclear envelopes break down, allowing direct merging of the haploid nuclei into a single diploid nucleus.^[1] In mammals, including humans, the pronuclei approach each other but do not fully fuse; instead, their envelopes interdigitate, and the chromosomes condense and align on a shared mitotic spindle during the first cleavage division, resulting in diploid daughter cells.^[3] This process typically occurs in the ampullary region of the fallopian tube in humans.^[2] Abnormalities in pronuclear formation or migration, such as asynchronous development, are associated with infertility and are key indicators in assisted reproductive technologies like in vitro fertilization.^[2]

Definition and Characteristics

General Definition

A pronucleus is the haploid nucleus derived from either the sperm or egg cell during fertilization, containing a single set of chromosomes that prepares the parental genetic material for fusion into the diploid zygote nucleus. In humans, each pronucleus carries 23 chromosomes, half the diploid number found in somatic cells.^[4]^[5] Upon fusion during syngamy, these combine to form the zygote nucleus with 46 chromosomes, marking the initiation of embryonic development.^[3] Pronuclei differ from the nuclei of mature gametes, which are produced through meiosis and remain in a condensed state until fertilization. These structures are transient, existing only briefly to facilitate the reorganization and alignment of genetic material prior to nuclear fusion.^[6] The formation and function of pronuclei represent a conserved feature of sexual reproduction across eukaryotes, occurring in diverse taxa such as animals, plants, and algae as an essential prelude to syngamy.^[7]^[8]

Structural Features

The pronucleus constitutes an enlarged haploid nucleus enveloped by a reformed nuclear membrane, enclosing decondensed chromatin organized into a less compact structure compared to the preceding gametic nuclei.^[1] This nuclear envelope, derived from oocyte endoplasmic reticulum components, integrates proteins such as lamins to provide structural support and facilitate nucleocytoplasmic transport, while the chromatin incorporates maternal histones and associated epigenetic modifiers.^[9] Within the pronucleus, nucleoli or nucleolar precursor bodies emerge as prominent substructures, clustering near the chromatin and containing ribosomal RNA precursors essential for early embryonic transcription.^[10] In mammals, the male pronucleus typically measures approximately 20-25 μm in diameter and arises from the sperm head, where protamine-packaged DNA undergoes rapid remodeling to histone-based chromatin supplied by the oocyte cytoplasm.^[11] This structure initially lacks embedded centrioles, which are contributed by the sperm but remain in the adjacent cytoplasm until post-fusion integration during syngamy.^[12] The male pronucleus often exhibits distinct epigenetic marks, such as elevated histone acetylation and reduced methylation on H3K9, contributing to its unique chromatin accessibility.^[13] The female pronucleus, by contrast, is similarly sized at around 20-30 μm in diameter in species like humans and mice, but features more prominent and numerous nucleoli due to maternal accumulation of ribosomal components.^[14] It harbors substantial maternal stores of RNAs and proteins, including factors like nucleoplasmin that aid in chromatin organization, and may appear asymmetrically shaped owing to its proximity to the oocyte's polar bodies and cytoplasmic determinants.^[15] This asymmetry influences nucleolar distribution and overall pronuclear morphology without affecting its core haploid composition.^[16] Pronuclei are readily observable in living mammalian zygotes through phase-contrast or differential interference contrast light microscopy, revealing their spherical to ovoid contours and internal nucleolar spots.^[17] For detailed chromatin visualization, fluorescent staining with DNA-binding dyes such as DAPI highlights decondensed patterns and nucleolar exclusion zones under epifluorescence or confocal microscopy, enabling non-invasive assessment of structural integrity.^[17]

Formation

Female Pronucleus Formation

The formation of the female pronucleus is initiated by the entry of the sperm into the oocyte, which triggers a series of signaling events including oscillatory increases in intracellular calcium levels that propagate as maternal calcium waves.^[18] These calcium signals induce cortical granule exocytosis, a process that releases enzymes into the perivitelline space to modify the zona pellucida and prevent polyspermy by hardening the egg coat.^[18] Concurrently, the calcium oscillations resume and drive the completion of the second meiotic division in the oocyte, which had been arrested at metaphase II.^[19] Upon completion of meiosis II, the oocyte extrudes the second polar body, retaining a haploid set of chromosomes in the ooplasm that subsequently decondense to form the female pronucleus.^[19] This decondensation involves the remodeling of the highly condensed oocyte chromatin, facilitated by maternal factors that promote histone modifications and chromatin relaxation, allowing the assembly of a functional nuclear envelope around the haploid genome.^[20] In mammals, this process is supported by the incorporation of maternal histone chaperones and variants, which ensure proper chromatin structure without the extensive protamine-to-histone exchange required in the male counterpart.^[21] The timeline for female pronucleus formation in mammals typically occurs within 3 to 10 hours post-fertilization, with species-specific variations; for instance, in mice, it forms around 7.5 hours after insemination, while in humans, it appears by approximately 8 hours on average.^[22]^[20] This developmental window involves microtubule reorganization to facilitate polar body extrusion and pronuclear assembly, coordinated by the calcium waves that sustain egg activation.^[19] Across species, the process exhibits notable variations. In sea urchins, where the oocyte completes meiosis prior to fertilization, the female pronucleus forms rapidly, within less than 30 minutes post-sperm entry, due to the pre-existing haploid state and swift decondensation.^[1]

Male Pronucleus Formation

Following sperm-egg fusion, the acrosome-reacted sperm penetrates the zona pellucida and delivers its haploid nucleus into the ooplasm, where initial structural changes occur to prepare for pronuclear development. The acrosome reaction, triggered by zona proteins, exposes enzymes that facilitate zona traversal and enables plasma membrane fusion with the egg.^[23] Upon entry, disassembly of the perinuclear theca—a cytoskeletal layer encapsulating the sperm nucleus—exposes the protamine-packaged DNA, marking the onset of nuclear remodeling. Decondensation of the sperm chromatin follows, involving the exchange of protamines for oocyte-derived histones to restore a nucleosomal structure compatible with transcription. This process is driven by oocyte factors, notably glutathione, which reduces disulfide bonds in protamines, allowing chromatin uncoiling and histone incorporation.^[1]^[24] A new nuclear envelope then assembles around the decondensed DNA, incorporating nuclear pores for transport. Key proteins such as importins facilitate nuclear pore complex assembly by regulating nucleoporin recruitment, while SMC proteins (structural maintenance of chromosomes) contribute to chromatin looping and higher-order organization during this reformation.^[25]^[26] Concurrently, the sperm's proximal centriole serves as the primary microtubule-organizing center, nucleating astral microtubules essential for subsequent zygotic events.^[27] In humans, male pronucleus formation is typically observed between 8-12 hours post-sperm entry, as seen in intracytoplasmic sperm injection (ICSI) procedures where decondensation begins around 4 hours in the ooplasm.^[28]^[29] This timeline varies across species; in mammals, oocyte glutathione is crucial for disulfide reduction and efficient decondensation, whereas in invertebrates like sea urchins, the process is faster—often under 1 hour—due to the acidic cytoplasmic environment that promotes protamine dissociation and histone phosphorylation.^[1]

Role in Fertilization

Pronuclear Migration

Pronuclear migration refers to the cytoskeletal-driven process in which the male and female pronuclei move toward each other within the zygote cytoplasm following fertilization, positioning them for subsequent syngamy. This movement is essential for the spatial alignment of parental genomes and is mediated primarily by microtubule networks organized by the sperm-derived centriole, which forms a radial aster to generate pulling forces on the pronuclei.^[19] Dynein motors, anchored to the cortex or organelles, walk along these microtubules to produce the necessary force for pronuclear translocation, often in coordination with microtubule polymerization and depolymerization dynamics.^[7] In mammalian zygotes, such as those of mice, pronuclear migration occurs in two distinct phases: a rapid peripheral phase driven by actin polymerization via Formin-2 and Spire nucleators, which propels the male pronucleus inward from the fertilization cone, followed by a slower central phase reliant on microtubule-dynein interactions for apposition.^[12] The sperm-introduced proximal centriole duplicates to form acentriolar microtubule-organizing centers (aMTOCs) that nucleate the microtubule aster, facilitating capture and transport of both pronuclei without a strict centriole dependency in all cases.^[19] This process typically unfolds over 4-8 hours post-fertilization in mice, with the male pronucleus starting farther from the cell center (approximately 37 μm) and migrating at initial velocities of 0.38 μm/min, slowing to 0.01 μm/min as it approaches the female pronucleus.^[12] Calcium signaling plays a regulatory role in pronuclear migration across species, with oscillatory calcium waves—often triggered by inositol 1,4,5-trisphosphate (IP3) receptors—promoting microtubule polymerization and aster formation shortly after fertilization. In mammalian eggs, these calcium oscillations, initiated by sperm phospholipase C zeta, sustain the cytoskeletal rearrangements needed for migration, while in sea urchins, a secondary calcium wave originating at the sperm entry point coincides with the onset of pronuclear movement.^[7]^[30] Species-specific variations highlight diverse cytoskeletal adaptations; in sea urchins, migration is microtubule-based via a rapidly maturing sperm aster, completing in under 30 minutes with velocities up to 4.9 μm/min, driven by dynein-mediated pulling without pronounced actin involvement in the central phase.^[19] In contrast, brown algae like Pelvetia, the female pronucleus remains anchored near the cell center by a stable microtubule and F-actin network, while the sperm pronucleus migrates toward it along fixed microtubule tracks, emphasizing a more static, directed pathway compared to the dynamic apposition in mammals.^[7] Time-lapse imaging techniques, including 3D confocal microscopy with fluorescent labels for chromatin (e.g., H2B-mCherry) and cell membranes (e.g., MyrGFP), have revealed synchronous rotation and approximation of pronuclei during migration, with the male pronucleus often rotating as it is pulled centrally in mammalian zygotes.^[12] These visualizations demonstrate coordinated oscillatory movements and highlight the precision of dynein-driven transport in aligning the pronuclei.^[31]

Syngamy and Genome Activation

Syngamy represents the culminating event of fertilization, wherein the male and female pronuclei approximate each other, their nuclear envelopes break down, and the parental chromosomes align on a shared mitotic spindle to achieve diploidy in the daughter cells of the first cleavage division. This process is triggered by the activation of maturation-promoting factor (MPF), which induces chromosome condensation and the assembly of a mitotic spindle apparatus. In mammalian zygotes, the spindle formation often involves dual structures that initially align maternal and paternal chromosomes separately before converging, ensuring proper chromosome segregation during the impending first mitotic division.^[32]^[33] The timeline of syngamy in humans typically occurs approximately 20 to 24 hours post-insemination, coinciding with the completion of DNA synthesis in the pronuclei. This is preceded by the disassembly of the pronuclear lamina, a meshwork of lamin proteins that supports the nuclear envelope, facilitating envelope breakdown and chromatin release into the shared cytoplasm. Prior to this, DNA replication initiates independently within each pronucleus during the S-phase, a process observed in species such as mice and humans, where replication licensing and origin firing occur asynchronously between parental genomes. Additionally, karyopherin-mediated nuclear import, which facilitates protein entry into the pronuclei during their formation, halts as the envelopes disassemble, marking the transition to mitotic events.^[22]^[34]^[35]^[36] Concomitant with syngamy is the onset of zygotic genome activation (ZGA), part of the broader maternal-to-zygotic transition (MZT) that shifts developmental control from maternal transcripts to embryonic gene expression. Recent research as of 2025 indicates that ZGA initiates at the one-cell stage in both mice and humans, with low-level transcription detectable shortly after fertilization, followed by a minor wave during the pronuclear stage that involves activity primarily from the male pronucleus in mice. The major wave follows at the 2-cell stage in mice and 4- to 8-cell stage in humans. The successful alignment and processing during syngamy terminates the pronuclear phase and initiates embryonic cleavage, with the integrated genome now poised for robust transcriptional activation.^[37]^[38]

Historical Development

Early Discoveries

The discovery of the pronucleus built upon foundational 19th-century studies of gametes, notably Karl Ernst von Baer's 1827 identification of the mammalian ovum through microscopic examination of ovarian follicles in dogs and other mammals.^[39] This breakthrough shifted embryological research toward understanding egg structure and fertilization processes in animals.^[39] In 1875, Belgian cytologist Edouard Van Beneden advanced this field by observing pronuclei in the oocytes of rabbits and bats using light microscopy on fixed specimens.^[40] His work, detailed in a paper on egg maturation and early embryonic development, described the formation of a peripheral male pronucleus from sperm material diffusing through the egg membrane and a central female pronucleus, which subsequently fused to form the first embryonic nucleus.^[40] Van Beneden's observations linked these pronuclei to the broader context of egg maturation, foreshadowing connections to meiotic processes observed in his later studies.^[40] One year later, in 1876, German zoologist Oscar Hertwig provided the first clear visualization of pronuclear fusion during fertilization in echinoderms through experiments on sea urchin eggs.^[41] By combining sperm and eggs in seawater and observing the process under a microscope—exploiting the transparency of sea urchin eggs—Hertwig documented the sperm pronucleus entering the egg and fusing with the female pronucleus, resolving debates on the role of sperm in heredity.^[41] These early efforts relied on rudimentary techniques such as direct microscopic viewing of living or freshly dissected material, basic fixation methods, and emerging nuclear staining to enhance visibility, without the benefit of molecular tools.^[41]^[40] Collectively, Van Beneden's and Hertwig's discoveries established pronuclei as distinct nuclear entities central to fertilization, transforming the view of the process from mere cell union to a precise nuclear merger essential for embryonic development.^[41]^[40]

Key Milestones

In the 1950s and 1960s, electron microscopy provided the first detailed views of pronuclear ultrastructure, revealing the decondensation of the sperm nucleus into a fibrous network within the egg cytoplasm, as observed in mammalian species like rabbits.^[42] Pioneering studies by C.R. Austin in 1961 described these processes, highlighting the transformation of the compact sperm head into a swollen pronucleus through chromatin remodeling and nuclear envelope reformation.^[43] During the 1970s, research on plant reproduction advanced models of double fertilization, where two sperm nuclei migrate to distinct targets in the embryo sac, forming separate pronuclei that fuse to initiate endosperm development in angiosperms.^[44] These cross-species investigations, using species like maize and lilies, elucidated pronuclear migration patterns and fusion mechanisms, contrasting with animal systems and informing evolutionary comparisons.^[44] The 1980s marked the IVF era, where pronuclei served as visible markers of successful fertilization in human embryos, with early observations documenting their formation and symmetry within 18-24 hours post-insemination.^[45] Studies in 1982 by teams including Trounson and colleagues reported the timing of pronuclear appearance in cultured human zygotes, enabling non-invasive assessment of fertilization viability.^[46] In the 1990s and 2000s, molecular insights deepened, with 1993 experiments on the alga Pelvetia demonstrating calcium signaling waves that trigger pronuclear formation and migration during fertilization.^[47] Concurrently, research in the 2000s uncovered the mechanisms of protamine removal from the male pronucleus, linking it to epigenetic reprogramming via glutathione-mediated disulfide bond reduction and histone reassembly.^[48] Post-2010 advancements included CRISPR/Cas9 applications, where pronuclear injections into zygotes enabled targeted genome editing to study paternal and maternal contributions, as shown in 2015 human tripronuclear zygote experiments achieving high-efficiency mutations.^[49] Live-cell imaging techniques in 2015 further revealed the role of mammalian centrioles in pronuclear migration, illustrating microtubule-dependent centering of the male pronucleus toward the female.^[50]

Clinical and Research Significance

In Assisted Reproduction

In assisted reproduction, particularly in vitro fertilization (IVF), the observation of pronuclei serves as a critical indicator of successful fertilization. Approximately 16-18 hours after insemination, embryologists assess oocytes for the presence of two distinct pronuclei (2PN), which signifies normal monospermic fertilization where one sperm has penetrated the oocyte, leading to the decondensation of the sperm head into the male pronucleus and the formation of the female pronucleus.^[51]^[52] The appearance of exactly two pronuclei is considered the ideal marker for selecting embryos suitable for transfer or further culture, as it confirms the extrusion of the second polar body and the initiation of syngamy.^[53] In intracytoplasmic sperm injection (ICSI) procedures, this assessment similarly verifies successful sperm injection, though ICSI typically exhibits lower rates of polyspermy compared to conventional IVF.^[54] The evaluation of pronuclei extends beyond mere counting to include morphological characteristics such as size symmetry and the pattern of nucleolar precursor bodies (NPBs), which provide predictive insights into embryo viability and implantation potential. Zygotes with symmetrical pronuclei and evenly distributed, large NPBs (e.g., in a polarized pattern) are associated with higher developmental competence and improved implantation rates.^[55] A study evaluating pronuclear morphology in conjunction with subsequent embryo grading demonstrated that selecting based on these features significantly enhances implantation success.^[56] These assessments help prioritize embryos for transfer, reducing the risk of selecting non-viable ones in cycles where multiple oocytes are fertilized. Non-invasive techniques, such as time-lapse imaging systems integrated into incubators, have revolutionized pronuclei monitoring by allowing continuous observation without removing embryos from optimal culture conditions. These systems capture images at intervals of 5-20 minutes, enabling the tracking of pronuclear formation, alignment, and disappearance in real-time, which correlates with subsequent cleavage timing and blastocyst quality.^[57]^[58] By minimizing environmental disturbances, time-lapse monitoring improves the accuracy of fertilization confirmation and supports dynamic embryo selection algorithms that integrate pronuclear dynamics with later morphokinetic parameters. Recent advancements as of 2025 include AI-based analysis of pronuclear patterns to further refine viability predictions.^[59]^[60] Abnormal pronuclear configurations, such as the presence of three or more pronuclei (3PN), are indicative of polyspermy, where multiple sperm enter the oocyte, and such zygotes are routinely discarded due to their high risk of chromosomal abnormalities and poor developmental outcomes.^[61] In human IVF cycles, approximately 70-80% of mature oocytes achieve normal fertilization as evidenced by the formation of two pronuclei, reflecting standard laboratory benchmarks for successful insemination rates.^[62] This statistic underscores the efficiency of modern protocols while highlighting the need for careful pronuclear evaluation to optimize cycle success.

Abnormalities and Implications

One common abnormality in pronucleus formation is polyspermy, where more than one sperm fertilizes the oocyte, resulting in multiple male pronuclei (typically three or more in IVF settings). This occurs due to failure of the cortical reaction, a key polyspermy-blocking mechanism involving exocytosis of cortical granules that modifies the zona pellucida to prevent additional sperm penetration.^[61]^[63]^[64] Polyspermy leads to triploid zygotes with an extra paternal genome set, causing embryonic arrest at early cleavage stages and inviable development.^[65]^[66] Asynchronous pronuclear development, characterized by delayed formation or differing sizes of the male and female pronuclei, often stems from dysregulation of calcium oscillations essential for oocyte activation and pronuclear decondensation. Genetic defects in sperm factors, such as mutations in PLCZ1 encoding phospholipase C zeta, can impair these oscillations, leading to incomplete activation and asynchrony.^[67]^[68]^[69] Such abnormalities reduce embryo viability, increasing rates of implantation failure and early arrest.^[70] Structural anomalies in pronuclei, including fragile nuclear envelopes and uneven chromatin distribution, are frequently linked to sperm chromatin defects like protamine deficiency, which disrupt proper decondensation and reprogramming during pronuclear formation. These issues can precipitate epigenetic errors, such as aberrant DNA methylation at imprinted loci, contributing to imprinting disorders like Beckwith-Wiedemann or Silver-Russell syndromes in ART-conceived offspring.^[71]^[72]^[73] In research, pronucleus abnormalities serve as models for studying aneuploidy mechanisms, as multipronucleate zygotes often exhibit chromosomal imbalances that mimic early embryonic errors. Clinically, they elevate miscarriage risk, accounting for a significant portion of IVF cycle failures due to non-viable embryos.^[74]^[75] These abnormalities are more prevalent in vitro than in vivo, where natural barriers limit sperm access; for instance, sea urchin eggs employ a rapid electrical fast-block depolarization to prevent polyspermy, a mechanism less emphasized in mammals but complemented by zona modifications.^[76]^[77]

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The pronuclei are usually checked between 16-18 hours after the sperm is added to the egg. 2PN. The 2PN will eventually disappear with the union of two gametes ...
[51]
Observation of pronuclei may not be an absolute indicator for ... - NIH
Upon entering the oocyte cytoplasm, the sperm nucleus undergoes a dramatic morphological transformation as the male pronucleus is formed and synthesizes DNA.
[52]
The Chromosomal Constitution of Embryos Arising from ... - NIH
Uniparental diploidy may sometimes occur if one pronucleus fails to develop and the other pronucleus already contains a diploid genome or alternatively a ...
[53]
Time-lapse monitoring of fertilized human oocytes focused on the ...
Sep 22, 2021 · The appearance of pronuclei (PN) in oocytes is widely used to assess fertilization in human in vitro fertilization (IVF) programs. Oocytes ...
[54]
Time-lapse imaging: Morphokinetic analysis of in vitro fertilization ...
Time-lapse imaging (TLI) allows continuous monitoring of embryo development without disturbing culture conditions by removing embryos from the incubator.
[55]
Three pro-nuclei (3PN) incidence factors and clinical outcomes - NIH
... sperm abnormality [24]. In this study, we selected semen of donors to exclude abnormal sperm influence as much as possible in IVF-ET cycles. When 3PN ...
[56]
Total fertilization failure: is it the end of the story? - PMC - NIH
... fertilization (IVF) laboratories, fertilization rates approach 70–80 % [1]. However, fertilization failure still exists as a frustrating experience. Not ...
[57]
Mutations in PLCZ1 induce male infertility associated with ... - NIH
Polyspermy is a major cause of multipronuclear fertilization, with up to 80% of multipronuclear fertilization resulting from polyspermic fertilization [5, 6].
[58]
Preventing polyspermy in mammalian eggs—Contributions of the ...
Mar 27, 2020 · The egg's blocks to polyspermy (fertilization of an egg by more than one sperm) were originally identified in marine and aquatic species ...
[59]
Prognostic value of triploid zygotes on intracytoplasmic sperm ...
Triploidy formation resulting from standard IVF insemination could be due to polyspermy (diandric causes). In the present study, we minimize diandric causes of ...
[60]
Influencing factors of three pronuclei incidence and their impact on ...
Multiple mechanisms prevent polyspermy to ensure normal fertilization. However, despite these safeguards, the formation of 3PN zygotes can still occur in some ...
[61]
Fertilization, Oocyte Activation, Calcium Release and Epigenetic ...
Mar 4, 2022 · Calcium signaling parameters, particularly amplitude, are important determinants of postfertilization development (Ozil and Huneau, 2001).
[62]
IVF: Oocyte postmaturity and pronucleus size asynchrony after ICSI
The cause of fertilization failure after ICSI may be related to ooplasmic immaturity or suboptimal activation capacity of the spermatozoa, both of which result ...Missing: defects | Show results with:defects
[63]
Defects in phospholipase C zeta cause polyspermy and low ... - NIH
Mutations in PLCZ1, the gene encoding PLCζ, cause male infertility and intracytoplasmic sperm injection (ICSI) fertilization failure.<|separator|>
[64]
Assessing the clinical viability of micro 3 pronuclei zygotes - PMC
The origin of 1PN zygotes may be due to parthenogenetic oocyte activation or asynchronous pronuclear formation and have been linked with implantation failure, ...
[65]
Novel insights into the genetic and epigenetic paternal contribution ...
During fertilization, the sperm transmits not only nuclear DNA to the oocyte but also activation factor, centrosomes, and a host of messenger RNA and microRNAs.
[66]
Pronuclear epigenetic modification of protamine deficient human ...
Aug 6, 2025 · Epigenetic abnormalities and abnormal chromatin structure in sperm may lead to male infertility. Protamine deficiency is among the disorders ...
[67]
Assisted reproduction treatment and epigenetic inheritance
At the level of genomic imprinting involving CpG methylation, ART-induced epigenetic defects are convincingly observed in mice, especially for placenta, and ...
[68]
The Frequency of Chromosomal Euploidy Among 3PN Embryos - PMC
In the IVF laboratory, pronuclear status, subsequent development rate and embryo morphology are common parameters in the selection of good quality embryos.Missing: implications | Show results with:implications
[69]
Association between clinical and IVF laboratory parameters and ...
Dec 14, 2021 · Several studies have shown that embryo aneuploidy is the main contributing factor to failed IVF, reinforcing the relevance of PGT-A as a means ...Missing: implications | Show results with:implications
[70]
Polyspermy prevention: facts and artifacts? - PMC - NIH
Polyspermy is a fact in aged oocytes in vivo and in vitro, whereas the concept of “polyspermy-blocking mechanisms” in mammalian oocytes is mainly based on ...
[71]
Ion channels and signaling pathways used in the fast polyspermy ...
Mar 1, 2021 · The fast polyspermy block uses a fertilization-activated depolarization of the egg membrane to electrically inhibit supernumerary sperm from entering the egg.<|control11|><|separator|>