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Rheumatoid factor

Rheumatoid factor (RF) is an autoantibody that targets the Fc portion of immunoglobulin G (IgG) molecules, serving as a serological marker primarily associated with rheumatoid arthritis (RA), a chronic autoimmune disease characterized by synovial inflammation and joint damage.^[1] Discovered in the 1940s through serological tests on RA patients, RF is produced by B cells and can form immune complexes that contribute to tissue injury in affected individuals.^[2] RF exists in multiple isotypes, with IgM being the most commonly detected and clinically relevant due to its prevalence in diagnostic assays, though IgG, IgA, IgE, and IgD variants also occur.^[1] In healthy individuals, low levels of RF may appear transiently in response to infections or immune challenges, such as those involving Epstein-Barr virus or bacterial antigens, where it aids in clearing immune complexes via phagocytosis.^[1] However, in RA, RF often exhibits higher affinity and monoclonal expansion, promoting persistent inflammation and correlating with disease severity.^[2] Detection of RF typically involves blood tests like nephelometry, turbidimetry, or enzyme-linked immunosorbent assay (ELISA), with normal levels generally below 20 IU/mL; elevated titers support RA diagnosis but lack specificity, as RF positivity occurs in 5-10% of healthy populations and up to 70% of cases in other autoimmune conditions like Sjögren's syndrome.^[2] Clinically, RF seropositivity—particularly when combined with anti-citrullinated protein antibodies (ACPAs)—enhances diagnostic accuracy per American College of Rheumatology/European League Against Rheumatism criteria, predicts erosive joint disease, and indicates a higher risk of extra-articular manifestations such as rheumatoid nodules or vasculitis.^[1] High RF levels are also linked to poorer prognosis in RA, influencing treatment decisions toward more aggressive therapies.^[2]

Definition and Pathophysiology

Definition

Rheumatoid factor (RF) is defined as a group of autoantibodies that target the Fc portion of the constant region of immunoglobulin G (IgG) molecules, classifying it as an autoantibody within the immune system.^[2] Primarily, RF consists of immunoglobulins of the IgM class, though isotypes including IgG, IgA, and IgE have also been identified and detected through enzyme-linked immunosorbent assays (ELISA) in clinical settings.^[1] These autoantibodies bind to self-IgG, distinguishing RF from other immunoglobulins by its autoreactive nature against the host's own antibody components.^[3] RF can occur in either polyclonal or monoclonal forms, with the polyclonal variant typically associated with broader immune responses in autoimmune conditions, while monoclonal RF arises in lymphoproliferative disorders.^[4] Among the isotypes, IgM-RF is the most commonly measured in laboratory assays due to its high prevalence, greater sensitivity in detection methods, and stronger association with disease markers compared to other forms.^[5] This focus on IgM-RF facilitates routine clinical evaluation, as it represents the predominant circulating form in affected individuals.^[1] In terms of detailed classification, RF specifically recognizes epitopes at the CH2-CH3 interface of the IgG Fc region, a critical structural domain that enables the autoantibody to cross-link multiple IgG molecules and promote the formation of immune complexes.^[6] This binding specificity underscores RF's role in initiating immune responses, though its full pathophysiological impact involves broader inflammatory processes.^[7]

Pathophysiological Mechanisms

Rheumatoid factor (RF) is primarily produced by plasma cells within lymphoid tissues, such as lymph nodes and bone marrow, as a result of B-cell activation triggered by exposure to altered forms of self-immunoglobulin G (IgG). This activation often occurs in response to post-translational modifications of IgG, including citrullination, which generates neoepitopes that breach immune tolerance and stimulate autoreactive B cells to differentiate into RF-secreting plasma cells.^[8] The process involves somatic hypermutation and affinity maturation in germinal centers, leading to the production of predominantly IgM-class RF, though IgG and IgA isotypes can also emerge.^[1] Once produced, RF binds to the Fc region of normal IgG molecules, forming soluble or tissue-deposited immune complexes that play a central role in propagating autoimmune pathology. These RF-IgG complexes activate the classical complement pathway, depositing C3 and C4 fragments that amplify inflammation by promoting opsonization and chemotaxis. Simultaneously, the complexes engage Fcγ receptors on macrophages and other innate immune cells, triggering phagocytosis, oxidative bursts, and the release of pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6). This cascade sustains chronic inflammation by recruiting additional leukocytes and fostering a self-perpetuating inflammatory microenvironment.^[8]^[1] The persistent immune activation driven by RF immune complexes contributes to key pathological changes in synovial tissues, including synovial hyperplasia characterized by fibroblast-like synoviocyte proliferation and angiogenesis. This hyperplastic synovium evolves into an invasive pannus that erodes cartilage and subchondral bone through matrix metalloproteinase secretion and osteoclast activation.^[9] Notably, high-avidity RF, which exhibits enhanced binding strength due to affinity maturation, demonstrates greater pathogenicity compared to low-avidity forms; the former correlates strongly with the progression to erosive joint disease, while low-avidity RF tends to be transient and less destructive.^[1]^[10]

Clinical Significance

Association with Rheumatoid Arthritis

Rheumatoid factor (RF) is detected in approximately 70-80% of patients with rheumatoid arthritis (RA), with positivity rates reaching up to 85% within the first two years of disease onset. Higher RF titers, particularly those exceeding 1:160, are associated with more severe and erosive disease, as well as increased risk of extra-articular manifestations such as rheumatoid nodules and vasculitis. These elevated levels serve as markers of aggressive disease course, contributing to greater joint destruction and systemic involvement in seropositive patients.^[11]^[12]^[13]^[1] In the 2010 American College of Rheumatology (ACR)/European League Against Rheumatism (EULAR) classification criteria for RA, RF plays a key role in diagnostic scoring, where serology is evaluated alongside joint involvement, symptom duration, and acute-phase reactants. A low-positive RF titer (above the upper limit of normal but ≤3 times the upper limit) scores 2 points, while a high-positive titer (>3 times the upper limit of normal) scores 3 points, enhancing the criteria's ability to identify definite RA when combined with clinical features. This integration improves diagnostic accuracy, particularly in early disease, by weighting RF positivity according to its strength and specificity.^[14]^[15] RF positivity also holds significant prognostic value in RA, with seropositive patients exhibiting worse radiographic progression, including higher rates of joint erosions and damage as measured by scores like the Sharp/van der Heijde method. Specifically, IgM-RF levels show a modest positive correlation with Sharp/van der Heijde scores (r=0.22, p=0.01), reflecting greater structural damage over time. Moreover, RF-positive RA is linked to poorer responses to therapies such as tumor necrosis factor inhibitors, necessitating more intensive treatment strategies to mitigate progression. The interaction between RF and anti-citrullinated protein antibodies (ACPAs) further amplifies specificity in seropositive RA, as dual positivity potentiates disease severity and erosive outcomes compared to either marker alone.^[16]^[17]^[3]^[18]

Associations with Other Diseases

Rheumatoid factor (RF) is not specific to rheumatoid arthritis and can be detected in various other autoimmune diseases, underscoring its limited diagnostic utility in isolation. In Sjögren's syndrome, RF positivity occurs in 50-70% of primary cases, often correlating with more severe glandular involvement and extraglandular manifestations. Similarly, RF is present in 20-30% of patients with systemic lupus erythematosus (SLE), where it may contribute to immune complex formation and disease activity, though it does not predict specific organ involvement. In mixed connective tissue disease (MCTD), RF is positive in approximately 40-70% of patients, frequently alongside high-titer anti-U1-RNP antibodies, and is associated with overlapping features of scleroderma, SLE, and polymyositis. Infectious conditions can also induce transient RF production, typically as part of a polyclonal B-cell response to persistent antigens, with levels often resolving upon treatment of the underlying infection. Chronic hepatitis C virus infection is notably associated with RF in 40-70% of cases, particularly in those developing extrahepatic manifestations like arthritis or vasculitis, where RF may form immune complexes contributing to tissue damage. Subacute bacterial endocarditis frequently shows RF positivity in 30-50% of patients, linked to chronic bacteremia and immune activation. Tuberculosis elicits RF in 20-40% of cases, often transiently during active disease, while viral infections such as Epstein-Barr virus can trigger low-titer, short-lived RF as part of the acute immune response. Beyond autoimmune and infectious etiologies, RF appears in 5-10% of healthy elderly individuals without rheumatic disease, reflecting age-related immune dysregulation and polyclonal hypergammaglobulinemia. Associations with malignancies, such as lymphoma, occur in up to 20% of cases, where RF may arise from dysregulated B-cell clones and correlate with worse prognosis in certain subtypes. In cryoglobulinemia, RF is integral to pathogenesis, forming type II (monoclonal IgM RF with polyclonal IgG) or type III (polyclonal RF with polyclonal IgG) cryoglobulins in over 90% of mixed cases, leading to small-vessel vasculitis and organ involvement. In primary biliary cholangitis, RF positivity is observed in 40-65% of patients and can help differentiate it from other chronic liver diseases by suggesting an autoimmune overlap, though it remains non-diagnostic when used alone.

Laboratory Testing

Detection Methods

The detection of rheumatoid factor (RF) primarily relies on immunological assays that identify antibodies binding to the Fc portion of IgG molecules. The most common quantitative method is nephelometry or turbidimetry, which measures the light scattering or absorbance caused by immune complexes formed between RF and IgG-coated particles in solution. These automated techniques are widely used for quantifying IgM-RF levels in clinical laboratories, offering high throughput and precision with intra-assay variability typically around ±10%.^[19]^[20] Enzyme-linked immunosorbent assay (ELISA) serves as a key method for isotype-specific detection of RF, including IgM, IgG, and IgA variants, by immobilizing IgG antigens on a solid phase and using enzyme-conjugated secondary antibodies to produce a colorimetric signal proportional to RF concentration. ELISA is particularly effective for detecting low-affinity RF, as it does not require the multivalent binding needed for visible agglutination, and results are often calibrated to international units per milliliter (IU/mL) against WHO standards.^[19]^[21]^[2] Qualitative screening tests include latex agglutination, where latex particles coated with human IgG aggregate in the presence of RF, providing rapid results observable by the naked eye, and the Rose-Waaler test, a historical hemagglutination assay using sheep red blood cells sensitized with rabbit IgG to detect primarily IgM-RF through visible clumping. These agglutination-based methods are simple and cost-effective for initial detection but are less sensitive to low-titer or low-affinity RF compared to quantitative approaches.^[19]^[20]^[21] Advanced variants, such as laser nephelometry, enhance traditional nephelometry by employing laser light sources for greater sensitivity in measuring light scatter from RF-IgG complexes, making it suitable for detecting subtle elevations in RF across isotypes. In comparisons, agglutination tests like latex fixation excel at identifying high-titer RF quickly for screening but often miss low levels, whereas nephelometry and ELISA are preferred for monitoring due to their superior precision and ability to quantify results in IU/mL, with nephelometry showing the highest overall sensitivity (approximately 69%) among automated methods.^[19]^[20]^[19]

Sample Collection and Procedure

The sample for rheumatoid factor (RF) testing is venous blood, typically collected via venipuncture into a serum separator tube (SST) or a plain red-top tube to obtain serum. For adults, a blood volume of 5 to 10 mL is standard, yielding approximately 1 to 3 mL of serum after processing.^[22]^[23]^[24] No special patient preparation is required, including no need for fasting, and the collection can occur at any time without dietary or medication restrictions specific to the test.^[21]^[20] After collection, the blood is allowed to clot at room temperature for 30 to 60 minutes, followed by centrifugation at 3,000 rpm for 10 minutes to separate the serum from cellular elements. Hemolysis must be avoided during venipuncture and handling, as it can compromise test accuracy.^[25]^[26]^[27] Serum stability is 7 days at 2-8°C or 3 months when frozen at -20°C; samples should be transported refrigerated if not tested immediately. For isotype-specific RF assays, serum is preferred to plasma to prevent anticoagulant interference, though EDTA- or heparin-anticoagulated plasma is acceptable in some protocols.^[28]^[22]^[26] Lipemic, icteric, or hemolyzed samples require recollection to avoid false results due to interference with assay detection. Turnaround time in clinical laboratories is typically 1 to 2 days from specimen receipt.^[27]^[29]

Interpretation of Results

Normal and Abnormal Ranges

The normal range for rheumatoid factor (RF) in serum is typically less than 15 international units per milliliter (IU/mL) when measured by quantitative methods such as nephelometry or latex agglutination, though this can vary slightly by laboratory, with some establishing an upper limit of 20 IU/mL.^[30]^[31] For enzyme-linked immunosorbent assay (ELISA) methods, the upper limit of normal is often set at 20 relative units per milliliter (RU/mL).^[32] In qualitative or semi-quantitative assays using agglutination, results are reported as titers, with normal values generally below 1:80, and some labs using a cutoff of less than 1:40 for enhanced specificity.^[30]^[29] Abnormal RF results are classified based on quantitative levels or titers to indicate positivity strength, which can correlate with disease severity in relevant contexts. Low-positive results fall in the range of 15-40 IU/mL or titers of 1:80 to 1:160, moderate-positive at 40-80 IU/mL or 1:320, and high-positive above 80 IU/mL or titers of 1:640 or greater.^[33]^[31] These thresholds are not universal and depend on the assay's sensitivity, but they provide a framework for interpreting elevations beyond the normal range. Several factors influence RF reference ranges and test outcomes. Age is a key variable, with levels tending to be higher in individuals over 60 years, where up to 10-25% of healthy elderly may test positive due to age-related immune changes, prompting labs to adjust cutoffs upward for this population.^[33] Method sensitivity also plays a role; for instance, nephelometric assays may report slightly higher normal upper limits (e.g., <60 U/mL in some protocols) compared to ELISA, affecting comparability across tests.^[33] False-positive results occur in 1-5% of the healthy population, often linked to non-specific immune activation or technical interferences like hemolysis.^[34]^[35] RF measurements are standardized in international units (IU/mL) by the World Health Organization (WHO) using reference preparations, primarily for IgM-RF.^[36]

Clinical Implications and Limitations

A positive rheumatoid factor (RF) result supports the diagnosis of rheumatoid arthritis (RA) when correlated with clinical symptoms, such as persistent synovitis involving one or more joints for at least 6 weeks, as outlined in the 2010 ACR/EULAR classification criteria.^[1] High RF titers are associated with a more aggressive disease course, including increased risk of joint erosions, extra-articular manifestations, and poorer long-term outcomes, prompting clinicians to initiate aggressive disease-modifying antirheumatic drug (DMARD) therapy early to mitigate progression.^[2] Despite its diagnostic utility, RF testing has notable limitations due to low specificity, with positivity observed in approximately 25% of patients with systemic lupus erythematosus (SLE) and 40-75% of cases in Sjögren's syndrome.^[37] False-positive results are also common in infections like hepatitis C (up to 76% positivity), though these typically resolve following treatment of the underlying infection.^[1] Importantly, RF negativity does not exclude RA, as 20-30% of cases are seronegative, particularly in early disease stages.^[38] To enhance diagnostic accuracy, RF testing is often paired with anti-cyclic citrullinated peptide (anti-CCP) antibodies, which offer approximately 95% specificity for RA, and imaging modalities like ultrasound or MRI to confirm synovial inflammation.^[1] Different RF isotypes (e.g., IgA, IgG) may provide additional prognostic information, with IgA-RF linked to extra-articular disease.^[1] For monitoring disease activity, serial RF titers may provide some insight into progression but are less reliable than acute-phase reactants such as erythrocyte sedimentation rate (ESR) or C-reactive protein (CRP), and are not routinely recommended for this purpose.^[1] As of 2025, emerging multiplex assays that integrate RF isotypes (e.g., IgM, IgA, IgG) with other biomarkers, including anti-CCP and novel autoantibody profiles, are showing promise in improving RA diagnostic precision and risk stratification over traditional single-analyte tests.^[39]

History and Developments

Discovery

The rheumatoid factor (RF) was first identified in 1948 by Harry M. Rose and colleagues at Columbia University, who observed that sera from patients with rheumatoid arthritis (RA) caused agglutination of sheep red blood cells sensitized with rabbit immunoglobulin G (IgG). This discovery stemmed from serological studies aimed at detecting antistreptolysin O antibodies, during which Rose's team noted unexpected agglutination patterns specific to RA sera, distinguishing it from normal sera or those from patients with other conditions. Independently, Erik Waaler had reported a similar phenomenon in 1940 using human sera to agglutinate sheep erythrocytes coated with rabbit anti-sheep red blood cell antibodies, though his findings received limited attention due to wartime disruptions. In the mid-1950s, the Waaler-Rose test was standardized as a clinical diagnostic tool, involving the sensitization of sheep red blood cells with subagglutinating doses of rabbit IgG to detect RF-mediated hemagglutination, which proved more specific for RA than earlier nonspecific tests.^[40] During this period, in the late 1950s, Ernest C. Franklin and Henry G. Kunkel confirmed RF as an anti-IgG antibody through fractionation studies showing its reactivity with human gamma globulin, particularly after enzymatic or chemical alteration of IgG that exposed hidden antigenic determinants. Initially termed the "rheumatoid serum factor" or "rheumatoid agglutinating factor" in foundational reports, it was characterized as a high-molecular-weight macroglobulin capable of forming complexes with IgG. By the 1960s, RF was recognized as an autoantibody directed against the Fc portion of self-IgG, with studies demonstrating its role in forming circulating immune complexes that contributed to RA pathogenesis, including complement activation and tissue deposition; electrophoretic and chromatographic analyses identified IgM as the predominant isotype of RF, comprising pentameric 19S macroglobulins, while IgG and IgA isotypes were less common but also pathogenic.^[41] This shift marked a pivotal understanding of autoimmunity in RA, as RF's presence in synovial fluids and sera linked it to chronic inflammation.

Modern Research Advances

In the 1980s and 1990s, advancements in rheumatoid factor (RF) detection shifted from qualitative methods to quantitative assays, particularly rate nephelometry, which provided more precise measurements of RF levels and enabled better monitoring of disease progression in rheumatoid arthritis (RA).^[42] This transition improved the ability to detect subtle changes in RF concentrations, surpassing earlier techniques like latex agglutination in sensitivity and reproducibility.^[43] Concurrently, research in the 1990s elucidated the role of RF isotypes—primarily IgM, but also IgG and IgA—in mixed cryoglobulinemia, where type II and III cryoglobulins were identified as cold-precipitable immune complexes containing RF, often IgM, contributing to vasculitis and systemic symptoms.^[44]^[45] Genetic studies in the 1980s established a strong association between HLA-DR4 and RF-positive RA, with HLA-DR4 positivity observed in approximately 65% of RF-positive patients compared to lower rates in seronegative cases or controls, linking this allele to earlier disease onset and more severe seropositivity.^[46]^[47] By the 2010s, investigations into RF's mechanistic role highlighted its involvement in B-cell dysregulation, where elevated RF production by autoreactive B cells correlated with incomplete B-cell depletion and poorer clinical responses to rituximab, a B-cell depleting therapy, in RA patients.^[48]^[49] Recent research from 2020 to 2025 has explored RF's implications in post-COVID-19 autoimmunity, with studies showing increased RF positivity and higher incidence of RF-associated RA in individuals following SARS-CoV-2 infection, potentially due to molecular mimicry and dysregulated immune responses.^[50]^[51] Multiplex serological panels combining RF with anti-citrullinated protein antibodies (anti-CCP) have been emphasized in guidelines for early RA detection, enhancing diagnostic accuracy to over 90% in presymptomatic stages when both markers are present.^[52] In 2024, findings indicated that IgA-RF serves as a predictor of mucosal involvement in RA, associating with lung and gut inflammation and supporting the mucosal origin hypothesis of disease pathogenesis.^[53] Ongoing clinical trials as of 2025 are evaluating RF-targeted therapies, including B-cell directed interventions like AlloNK cells, to address RF-driven autoimmunity in refractory RA cases.^[54]

References

[1]
Rheumatoid Factor - StatPearls - NCBI Bookshelf
Jul 24, 2023 · Clinical Significance The presence, absence, titers, and isotypes of rheumatoid factors have important implications for the diagnosis and ...Introduction · Function · Issues of Concern · Clinical Significance
[2]
Rheumatoid Factors: Clinical Applications - PMC - NIH
Rheumatoid factors are antibodies directed against the Fc region of immunoglobulin G. First detected in patients with rheumatoid arthritis 70 years ago.
[3]
Rheumatoid Factor: Diagnostic and Prognostic Performance and ...
Feb 25, 2025 · Rheumatoid factor (RF) is the first autoantibody identified in rheumatoid arthritis (RA) which targets the fragment crystallizable (Fc) region ...<|control11|><|separator|>
[4]
Determination of antibodies in everyday rheumatological practice
Analyzing the reasons for elevated antibody concentrations, we must pay attention to whether they are the result of the presence of monoclonal or polyclonal ...
[5]
Autoantibodies in Rheumatoid Arthritis: Historical Background ... - NIH
It encompasses immunoglobulins of IgM, IgG, and IgA classes targeting the Fc-fragment of IgG. However, RF can be present in a variety of other rheumatologic ...
[6]
Structure–function analyses of a stereotypic rheumatoid factor ...
Rheumatoid factors (RFs) are autoantibodies that specifically recognize the fragment-crystallizable (Fc) region of IgG and have long been used as an important ...Missing: definition | Show results with:definition
[7]
What is rheumatoid factor? From screening to personalized ... - NIH
Jan 10, 2025 · The RF is a representative autoantibody against the crystallizable fragment (Fc) of denatured IgG that is primarily detected in patients with RA.
[8]
https://doi.org/10.1056/NEJMra1004965
[9]
https://doi.org/10.1038/s41413-018-0016-9
[10]
https://doi.org/10.1172/JCI60975
[11]
Rheumatoid factors in patients with rheumatoid arthritis - PMC - NIH
About 70% of RA patients test positive for RF at onset of RA, and 85% become positive during the first 2 years of the disease. Serum levels of RF do not change ...
[12]
Rheumatoid Factor and Anti-CCP Antibody in Rheumatoid Arthritis
Cohort studies reveal that about 60–80% of RA patients are sero-positive. These patients have specific clinical outcome and treatment response. Nowadays, we can ...
[13]
Rheumatoid factor is the major predictor of increasing severity of ...
However, after adjusting for baseline severity, a high titer of RF (>1:160) was also an independent predictor of deterioration over 5 years: individuals ...
[14]
The 2010 American College of Rheumatology/European League ...
The American College of Rheumatology and the European League Against Rheumatism have developed new classification criteria for rheumatoid arthritis (RA).
[15]
ACR/EULAR 2010 rheumatoid arthritis classification criteria
Negative means less than or equal to the upper limit of normal (ULN); low positive means >ULN; high positive means >3× ULN. cNormal and abnormal are ...
[16]
Effective factors on Sharp Score in patients with rheumatoid arthritis
Oct 9, 2021 · There was a higher sharp score of RF-positive compared to RF ... radiographic lesions and Sharp/van der Heijde score [12]. Similar ...
[17]
Levels of anti-citrullinated protein antibodies and IgM rheumatoid ...
ACPA and IgM-RF levels were modestly correlated with SHS (r = 0.12 and r = 0.11, respectively, both P = 0.01) and not with DAS28 and HAQ (Tables 1 and 2).
[18]
Rheumatoid factor as a potentiator of anti-citrullinated protein ... - NIH
We sought to evaluate the epidemiologic and molecular interaction of ACPA and RF with both disease activity as well as measures of RA-associated inflammation.
[19]
Rheumatoid factor isotypes in rheumatoid arthritis diagnosis and ...
Aug 4, 2023 · The main objective of this study is to provide an analysis of the literature on the role of RF isotypes in the diagnosis and prognosis of rheumatoid arthritis ...
[20]
Rheumatoid Factor (RF) Test: MedlinePlus Medical Test
Jun 9, 2025 · A rheumatoid factor (RF) test checks a sample of your blood for rheumatoid factor, a protein made by the immune system.
[21]
Rheumatoid factor - Mayo Clinic
Aug 26, 2023 · During a rheumatoid factor test, a member of your health care team will take a small sample of blood from a vein in your arm. This often takes ...Missing: techniques | Show results with:techniques
[22]
006502: Rheumatoid Factor (RF) - Labcorp
Rheumatoid Factor (RF) ; Specimen. Serum or plasma ; Volume. 1 mL ; Minimum Volume. 0.5 mL ; Container. Red-top tube, gel-barrier tube, lavender-top (EDTA) tube, or ...
[23]
RHEUMATOID FACTOR, Serum - Test Details
Storage/Transport: Delivered to Sample Management within 2 hours of collection. Specimen Preparation: Allow specimen to clot completely at room temperature.<|control11|><|separator|>
[24]
Rheumatoid Factor - WMCG Laboratory Test Catalog
WMCG Laboratory Test Catalog powered by Mayo Clinic Laboratories ... Specimen Requirements. 5 mL gold top SST tube 2 mL blood ...
[25]
Rheumatoid Factor (RF) - Lab Test | Corewell Health Laboratory
Mar 31, 2025 · Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Refrigerate (2-8°C or 36-46°F) the centrifuged ...Missing: procedure volume
[26]
Rheumatoid Factor, Serum or Plasma - Bronson Laboratory Services
After centrifugation, specimen tubes without a gel barrier should have ... Specimen Stability. Specimen Type, Temperature, Time. Serum*, Refrigerated, 8 days.
[27]
[PDF] CentraCare Laboratory Services
SPECIMEN CENTRIFUGATION & STABILITY GUIDE. Updated 10/21/25 LM. Chemistry ... Rheumatoid Factor. Serum. 4 hours. 3 days. 1 month. RPR. Serum. 4 hours. 5 days. >5 ...
[28]
[PDF] Quantitative determination of Rheumatoid Factors (RF) - Atlas Medical
Fresh serum. Stable 7 days at 2‐8⁰C or 3 months at -. 20⁰C. Samples with presence of fibrin should be centrifuged before testing ...
[29]
Rheumatoid Factor (RF) Test - Testing.com
Dec 6, 2022 · A blood sample is obtained for an at-home RF test by pricking a finger and collecting the sample as instructed in a test kit. Before the test.
[30]
Rheumatoid factor (RF) - UCSF Benioff Children's Hospitals
Apr 30, 2023 · Normal Results · Value, normal less than 15 IU/mL · Titer, normal less than 1:80 (1 to 80).
[31]
Rheumatoid factor: Ranges, tests, and treatment - MedicalNewsToday
The typical range for RF is usually 0–20 units per milliliter (U/ml) of blood. A person's risk of developing rheumatoid arthritis typically increases with their ...
[32]
Normal Rheumatoid Factor Levels - Johns Hopkins Arthritis Center
Mar 24, 2014 · Most laboratory's normal range is from 0.0 to 14.0. Any level higher than their normal range is abnormal.
[33]
Rheumatoid Factor: Reference Range, Interpretation, Collection and ...
Oct 16, 2025 · RFs are used as a marker in individuals with suspected rheumatoid arthritis (RA) or other autoimmune conditions. Their levels may also increase ...Interpretation · Background · Description
[34]
Rheumatoid Factor Positivity in the General Population
Sep 11, 2025 · Approximately 3-5% of the general population tests positive for Rheumatoid Factor (RF) without having rheumatoid arthritis, with prevalence ...
[35]
Rheumatoid Factor (Testing) - Patient.info
Rheumatoid factors are antibodies against the F portion of IgG (also an antibody). Rheumatoid factor can belong to any isotype of immunoglobulin ... IgE - and any ...
[36]
Rheumatoid factor (RF) - UnitsLab.com
Rheumatoid factor (RF) conversion calculator for units in scientific, laboratory and medical reports. Units conversion: IU/mL, U/mL, kU/L, kIU/L.Missing: standard μg/
[37]
Predicting rheumatoid arthritis progression from seronegative ... - NIH
Mar 26, 2025 · We observed in the KURAMA cohort that 27.1% of seronegative UA patients developed RA. Seropositivity in general population is associated with ...
[38]
https://pmc.ncbi.nlm.nih.gov/articles/PMC11938622/
[39]
The Waaler-Rose test: Anatomy of the eponym - ScienceDirect.com
M Mannik. Rheumatoid factors—their discovery and possible role in pathogenesis of rheumatoid arthritis ... Rheumatoid agglutinating globulin, JAMA, 138 (1948), p.
[40]
Studies on the isolation and characterization of the "rheumatoid factor"
Studies on the isolation and characterization of the "rheumatoid factor". J Clin Invest. 1959 Feb;38(2):424-34. doi: 10.1172/JCI103817.Missing: anti- IgG 1950s
[41]
Routine quantification of rheumatoid factor by rate nephelometry - NIH
The nephelometric method was considered superior compared with the other techniques because of its ability to detect changes in absolute levels at earlier ...Missing: quantitative 2000s
[42]
Routine quantification of rheumatoid factor by rate nephelometry
In a cross-sectional study of over 3000 consecutive serum specimens the levels of rheumatoid factor (RF) measured by rate nephelometry (Beckman ICS II) were ...
[43]
Rheumatoid Factor, Complement, and Mixed Cryoglobulinemia - PMC
Type 2 MCs are almost invariably composed of monoclonal IgM kappa RF and polyclonal IgG, and it is the complexing of the two that is a requisite for the ...
[44]
Cryoglobulinemia: Practice Essentials, Pathophysiology, Etiology
Apr 28, 2023 · Types II and III cryoglobulinemia (mixed cryoglobulinemia) contain rheumatoid factors (RFs), which are usually IgM and, rarely, IgG or IgA.Missing: isotypes 1990s
[45]
The association between rheumatoid arthritis and the HLA antigen ...
DR4 appeared to be associated with an early onset of RA. No significant differences in general disease activity or functional capacity between DR4-positive and ...
[46]
Associations of HLA-DR4 with rheumatoid factor and radiographic ...
Overall, 65 percent of the patients were HLA-DR4 positive, similar to other series. HLA-DR4 was associated significantly with the presence of rheumatoid factor ...
[47]
Rheumatoid arthritis patients display B-cell dysregulation already in ...
Dec 27, 2019 · Non-response to rituximab therapy in rheumatoid arthritis is associated with incomplete disruption of the B cell receptor repertoire. Ann ...Results · Ra B Cells Have Elevated... · Mass Cytometry Data Analysis<|separator|>
[48]
Non-response to rituximab therapy in rheumatoid arthritis is ...
Rituximab induces deep depletion of the B cells, but up to 35% of treated patients with rheumatoid arthritis (RA) do not achieve clinical European League ...Missing: dysregulation | Show results with:dysregulation
[49]
Rheumatoid arthritis-associated rheumatoid factors post-COVID-19
Feb 13, 2025 · Rheumatoid factors (RFs) are a hallmark of rheumatoid arthritis but also arise in infections, including COVID-19. Moreover, infections, again ...Figure 2 · Figure 3 · Figure 4
[50]
Risk of autoimmune diseases in patients with COVID-19 - The Lancet
Jan 9, 2023 · Our findings showed that the COVID-19 cohort exhibited significantly higher risks of rheumatoid arthritis, ankylosing spondylitis, systemic ...
[51]
Biomarkers for early diagnosis of rheumatoid arthritis - ScienceDirect
Jun 15, 2025 · The simultaneous detection of RF and ACPA enhances the accuracy of early RA diagnosis. Consequently, multiplex immunoassay techniques, capable ...Missing: panels | Show results with:panels
[52]
The prognostic value of IgA anti-citrullinated protein antibodies ... - NIH
Jul 3, 2024 · The mucosal origin hypothesis of rheumatoid arthritis has renewed the interest in IgA autoantibodies, but their added value over IgG ...
[53]
Artiva Biotherapeutics Announces Refractory Rheumatoid Arthritis ...
Oct 16, 2025 · AlloNK is currently being evaluated in three ongoing clinical trials for the treatment of B-cell driven autoimmune diseases, including a ...