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Root-knot nematode

Root-knot nematodes (Meloidogyne spp.) are microscopic, soil-borne, obligate plant-parasitic roundworms that rank among the most damaging agricultural pests globally, infecting the roots of over 2,000 plant species and inducing distinctive galls or "knots" through cellular manipulation to form specialized feeding sites known as giant cells.^[1]^[2] These sedentary endoparasites, typically measuring 0.3 to 1.5 mm in length, thrive in warm, sandy soils and complete their life cycle in 20–30 days under optimal conditions of 25–30°C, with second-stage juveniles (J2) serving as the primary infective stage that penetrates root tips.^[1]^[3] The genus Meloidogyne encompasses over 100 described species, with the most economically significant including M. incognita (southern root-knot), M. javanica, M. arenaria, and M. hapla (northern root-knot), which exhibit varying geographic distributions and host preferences—M. incognita predominating in tropical and subtropical regions, while M. hapla tolerates cooler climates.^[1]^[4] Females develop into swollen, pear-shaped adults embedded in root tissue, laying up to 1,000 eggs in gelatinous masses that hatch into J2 juveniles, enabling rapid population buildup; reproduction often occurs via parthenogenesis, enhancing their adaptability and spread through infested soil, water, or equipment.^[2]^[3] Infection leads to impaired root function, disrupting water and nutrient uptake, which manifests aboveground as stunting, wilting, yellowing foliage, and patchy crop growth, while belowground symptoms include beaded galls on roots and reduced root hair development, exacerbating susceptibility to secondary pathogens.^[1]^[2] Economically, root-knot nematodes are responsible for approximately 5% of global crop losses, contributing to total nematode-related damages of around USD 100 billion annually, particularly affecting high-value crops such as tomatoes, potatoes, cotton, soybeans, and ornamentals, with severe infestations capable of destroying entire fields in unmanaged systems.^[5] Their persistence in soil for months to years underscores the need for integrated management approaches, including resistant varieties, crop rotation, and soil solarization, to mitigate their widespread threat to food security and horticulture.^[3]^[2]

Taxonomy and description

Classification and species

Root-knot nematodes belong to the genus Meloidogyne within the phylum Nematoda, class Chromadorea, order Rhabditida, superfamily Tylenchoidea, family Meloidogynidae.^[6] This taxonomic placement reflects their position among plant-parasitic nematodes characterized by sedentary endoparasitism and gall-inducing behavior. The genus includes over 100 described species, of which approximately 10 to 12 are of major economic importance due to their widespread impact on agriculture.^[7] Key species include M. incognita, first described in 1919 by Kofoid and White based on eggs from human feces but later recognized as a plant parasite, though it is most notorious for infecting cotton (Gossypium spp.) in southern regions.^[8] M. javanica, originally noted as Heterodera javanica in 1885 by Treub from sugarcane in Java (Indonesia) and formally named in 1949 by Chitwood, is a tropical pathogen prevalent in warm climates.^[9] M. arenaria, described in 1889 by Neal from peanut (Arachis hypogaea) roots in Georgia, USA, is common in sandy soils and affects legumes and solanaceous crops. M. hapla, first described in 1949 by Chitwood from potato (Solanum tuberosum) roots in Long Island, New York, thrives in cooler temperate areas and infects a broad range of vegetables.^[10] Species delineation relies on morphological traits such as the female perineal pattern, which exhibits distinct striae and arch shapes unique to each species, supplemented by morphometrics of juveniles and males.^[11] Biochemical methods, including isozyme electrophoresis for esterase (EST) and malate dehydrogenase (MDH) profiles, provide species-specific banding patterns for rapid identification. Molecular techniques, such as sequence characterized amplified region polymerase chain reaction (SCAR-PCR) with species-specific primers targeting ribosomal DNA or mitochondrial regions, enable precise differentiation even from single juveniles.^[12] Root-knot nematodes likely originated in tropical regions, with diversification driven by adaptation to diverse hosts and climates.^[13] Phylogenetic analyses of mitochondrial DNA reveal the polyphyletic nature of parthenogenetic lineages within the genus, suggesting multiple independent evolutionary events involving interspecific hybridization and shifts in reproductive modes.^[14]

Morphology of life stages

Root-knot nematodes, belonging to the genus Meloidogyne, exhibit distinct morphological adaptations across their life stages that facilitate identification and understanding of their parasitic biology. The eggs are typically ellipsoid to oval, with an average length of 94.37 μm and width of 41.24 μm, encased in a transparent chitinous shell for protection. These eggs are deposited in clusters within a gelatinous matrix secreted by the adult female's rectal glands, composed primarily of mucopolysaccharides along with proteins and enzymes, which aids in adhesion to host roots and shields against environmental stresses like desiccation and microbial invasion.^[15]^[16] The juvenile stages include four molts, with the second-stage juvenile (J2) being the vermiform, infective form that hatches from the egg and migrates in soil to penetrate host roots. This stage measures 350–560 μm in length, featuring a slender, elongated body, a robust protrusible stylet approximately 10–15 μm long for tissue penetration, three prominent esophageal gland cells that produce enzymes for host invasion, and a tail with species-variable shapes often terminating in a short hyaline region of 6–13 μm. Later juveniles (J3 and J4) transition to a more sedentary, swollen morphology within the host but retain vermiform traits until adulthood.^[17]^[18] Adult females are sedentary endoparasites with a saccate, pear-shaped body ranging from 0.5–1.5 mm in diameter, characterized by a white, swollen posterior and a protruded neck region housing the stylet (13–17 μm long with rounded knobs). The vulva is large and posterior, positioned terminally, and the perineal region displays a distinctive cuticular pattern of arched striae—such as a high, squared dorsal arch in common species like M. incognita—essential for species differentiation. This pattern encompasses the vulva-anus area, phasmids, and lateral lines, varying in outline from rounded to ovoid across species.^[17]^[18] Adult males, in contrast, maintain a vermiform, cylindrical body 1–2 mm long, adapted for mobility, though they are rare or absent in parthenogenetic populations. They possess a stylet 18–25 μm long with prominent, rounded knobs, a bluntly rounded tail that may lack striations, paired spicules (25–33 μm), and a gubernaculum for copulation. The head often lacks an offset, with 0–5 annules anteriorly.^[17]^[18] Diagnostic features of root-knot nematodes include the absence of a persistent cyst stage, distinguishing them from cyst-forming nematodes like Heterodera spp., and reliance on microscopic examination for confirmation. Light microscopy reveals overall body shapes and stylet details, while scanning electron microscopy (SEM) elucidates fine cuticular patterns, such as perineal striae and tail mucro, enabling precise species identification without molecular methods.^[17]

Life cycle

Egg production and hatching

Female root-knot nematodes, primarily species in the genus Meloidogyne such as M. incognita, deposit eggs within specialized egg masses formed inside or on the surface of root galls after reaching maturity. Each female typically produces 300 to 1,000 eggs, depending on environmental conditions and host availability, with the eggs developing from zygote to the embryonated second-stage juvenile (J2) within 7 to 10 days at optimal temperatures of 25 to 30°C.^[19]^[20] The eggs are encased in a protective gelatinous matrix secreted by the female's rectal glands, which serves to shield them from desiccation, microbial predators, and environmental stressors while facilitating clumping to maintain proximity to the host root. This matrix, composed primarily of proteins and polysaccharides, adheres the egg mass to the root tissue and may contain enzymes like cellulases that aid in root penetration for subsequent infections.^[6] Hatching of J2 larvae from the embryonated eggs is primarily driven by environmental cues, with optimal temperatures ranging from 20 to 30°C promoting rapid emergence, while extremes below 10°C or above 35°C inhibit development and hatching. Adequate soil moisture is essential, as water uptake through the eggshell is required for J2 motility and rupture of the eggshell via enzymatic and physical mechanisms; low moisture leads to delayed or arrested hatching. Root exudates, including amino acids and sugars from susceptible host plants, can stimulate J2 emergence by enhancing eggshell permeability, though exudates from resistant varieties often suppress hatching rates by up to 66%.^[20]^[21]^[22]^[23] Additional factors influencing hatching include soil oxygen levels, which support aerobic respiration during J2 activation, and pH in the range of 5 to 7, where neutral to slightly acidic conditions maximize hatch rates. Inhibitors such as ammonia or certain soil microbes can reduce hatching by disrupting eggshell integrity or J2 viability, underscoring the role of soil chemistry in regulating population dynamics.^[24]^[25]

Juvenile migration and infection

Upon hatching, second-stage juveniles (J2) of root-knot nematodes (Meloidogyne spp.) initiate active migration through the soil to locate suitable host roots, relying on their limited energy reserves as non-feeding stages. These vermiform J2, measuring approximately 0.4–0.5 mm in length, move sinuously within thin water films adhering to soil particles, achieving distances of up to 30 cm or more under optimal conditions.^[26]^[27] Migration is primarily directed by chemotaxis, where J2 respond to gradients of host root exudates, including carbon dioxide (CO₂), amino acids, and volatile organic compounds, which serve as long-range attractants to guide them toward infection sites.^[28]^[29] Without a host, J2 survival is limited, persisting for several months in moist soil but declining rapidly to weeks in dry conditions due to desiccation and starvation, with populations often reducing by 95–99% over three months in warm, bare fallow soil.^[26]^[30] Environmental factors significantly influence J2 motility and dispersal. Sandy soil textures facilitate greater movement compared to clay-rich soils, as coarser particles provide larger pore spaces and better water film continuity for navigation.^[31]^[32] Temperature plays a critical role, with optimal migration occurring between 20–26°C; activity slows markedly below 15°C and halts entirely below 10°C, as metabolic processes and hatch-related motility are inhibited at these thresholds.^[33]^[34] Soil moisture must be adequate (typically above -2 bars suction) to support mobility, but excessive water flow can displace J2 unless they seek refuge in soil pores.^[35] Once a host root is located, typically near the tip, the J2 penetrates the epidermis using repeated thrusts of its hollow stylet, a needle-like mouthpart adapted for both mechanical puncture and secretion delivery.^[6] The juvenile then migrates intercellularly through the root cortex toward the vascular cylinder, avoiding direct cell penetration to minimize resistance, while secreting cell wall-degrading enzymes such as cellulases (β-1,4-endoglucanases) and pectinases (pectate lyases) from its esophageal glands to soften tissues and facilitate passage.^[6]^[36] This enzymatic activity, combined with stylet probing, enables the J2 to navigate efficiently without causing immediate visible damage like galls. Establishment begins as the J2 selects a permanent feeding site in the root's zone of elongation or maturation, where actively dividing cells provide optimal nutrients.^[37]^[38] Here, the nematode inserts its stylet into a single cell, initiating secretions that will later induce cellular changes, though no galls form at this early penetration stage.^[39] The J2's stylet, equipped with a conus for piercing, ensures precise targeting, and its brief motile phase post-penetration transitions it to sedentism within hours to days, depending on host response and environmental cues.^[6]

Root gall formation and maturation

Upon penetration into the root, the second-stage juvenile (J2) of root-knot nematodes, such as Meloidogyne incognita, selects vascular parenchyma cells and induces their transformation into multinucleate giant cells through the injection of esophageal gland secretions via its stylet.^[37] These secretions include effector proteins that modify host cell walls and manipulate cellular processes, leading to repeated rounds of karyokinesis without cytokinesis, resulting in enlarged cells with multiple nuclei.^[40] Specifically, nematode-derived β-1,4-endoglucanases contribute to partial cell wall dissolution and remodeling, facilitating the expansion of the selected cell into a multinucleate giant cell through endoreduplication and acytokinetic mitosis, forming a cohesive feeding structure rich in nutrients and phloem-like transfer cells.^[40]^[41] The maturation of giant cells occurs progressively over 10 to 20 days post-infection, during which the nematode remains sedentary, feeding continuously and molting from J2 to the third (J3) and fourth (J4) juvenile stages within the protective gall.^[37] As the nematode enlarges, reaching up to 0.5 mm in length by the adult stage, the surrounding root tissues undergo hyperplasia and hypertrophy, forming visible bead-like galls that can measure 2 to 10 mm in diameter depending on species and host.^[37] The full development from J2 to mature adult female typically spans 20 to 30 days under optimal temperatures of 25–30°C, with the female becoming a sedentary, globose structure embedded in the gall.^[41] In species capable of amphimixis, such as M. hapla, a subset of juveniles undergoes sex determination during the J3 or J4 stage, molting into vermiform males that migrate out of the root to mate with females on the surface.^[42] However, many root-knot species, including M. incognita, predominantly reproduce via mitotic parthenogenesis, rendering males rare and unnecessary for reproduction.^[43] Physiologically, the giant cells divert nutrients and water from the host plant by acting as strong sinks, supported by nematode effectors that mimic or enhance plant hormones such as auxin and cytokinin.^[44] These hormonal manipulations, including the secretion of conjugated auxins and cytokinins by the nematode, promote localized cell proliferation and inhibit differentiation, thereby sustaining gall enlargement and feeding site functionality.^[44]

Reproduction strategies

Root-knot nematodes (Meloidogyne spp.) primarily reproduce through parthenogenesis, an asexual process that results in predominantly all-female populations and enables rapid proliferation in host roots.^[45] This mode dominates in most species, with two distinct forms: mitotic parthenogenesis (apomixis), where oocytes undergo mitotic divisions without meiosis, producing genetically identical clonal progeny, and meiotic parthenogenesis (automixis), involving meiotic reduction followed by diploid restoration through chromosome reunification or central fusion, which allows limited recombination but leads to rapid genomic homozygosity.^[45]^[46] Mitotic parthenogenesis is characteristic of widespread tropical species such as M. incognita, M. javanica, and M. arenaria, often associated with polyploidy (e.g., triploid with somatic chromosome numbers of 40–48), while meiotic parthenogenesis occurs in temperate species like M. hapla (facultative in many isolates) and M. chitwoodi.^[45]^[47]^[46] Sexual reproduction via amphimixis, involving male-female mating and fertilization, is less common and limited to a few species such as M. carolinensis and M. floridensis, where males transfer sperm to oocytes, promoting genetic diversity through recombination and outcrossing.^[45]^[47] In parthenogenetic species, males are rare and typically non-functional for reproduction, arising sporadically under environmental stress like high population density or poor nutrition, potentially serving roles in population regulation rather than fertilization.^[45]^[46] However, in facultative meiotic species like M. hapla, males can participate in occasional sexual crosses, fertilizing eggs to produce heterozygous offspring and enhancing adaptability.^[46] The benefits of sexual reproduction include increased genetic variability, which aids in overcoming host resistance and environmental challenges, contrasting with the clonal stability of parthenogenesis.^[47]^[48] A single mature female root-knot nematode typically produces 300 to 1,000 eggs over her lifetime, deposited in a gelatinous matrix within root galls, supporting 1 to 3 generations per crop cycle under optimal conditions (20–30 days per generation at 25–30°C).^[43] Mitotic parthenogens generate uniform clonal lines, limiting long-term diversity but enabling swift population expansion, whereas meiotic forms permit some recombination, contributing to moderate variability within lineages.^[45]^[46] Evolutionarily, the shift to parthenogenesis in root-knot nematodes likely arose recently through interspecific hybridization and whole-genome duplication, facilitating rapid colonization of diverse hosts and environments compared to ancestral amphimictic forms.^[47]^[48] Cytogenetic studies reveal a basic haploid chromosome number of 18, with somatic complements varying from diploid (2n ≈ 36) to triploid or higher (up to 54) due to aneuploidy and polysomy, particularly in mitotic species, underscoring adaptations for genetic stability and plasticity.^[45]^[47] These mechanisms have enabled parthenogenetic lineages to dominate agricultural pests, with evidence of adaptive evolution such as virulence against resistant crops emerging in few generations.^[45]

Hosts and pathology

Host range and susceptibility

Root-knot nematodes (Meloidogyne spp.) exhibit an exceptionally broad host range, parasitizing over 2,000 plant species across more than 100 families worldwide.^[49] Dicotyledonous plants are predominantly susceptible, including major crops such as tomatoes (Solanum lycopersicum), peppers (Capsicum spp.), and cotton (Gossypium hirsutum), while monocotyledonous plants like corn (Zea mays) are often resistant or serve as poor hosts with limited nematode reproduction.^[49] As obligate biotrophs, these nematodes lack a free-living phase and depend entirely on living host tissues for survival and development, restricting their interactions to compatible plant hosts.^[50] Host preferences vary by species, with M. incognita favoring warm-season crops in tropical and subtropical regions, whereas M. hapla thrives on temperate hosts and exhibits a narrower range overall.^[49] Susceptibility is influenced by chemical signals from root exudates that attract infective second-stage juveniles (J2) to host roots, as well as plant genetic factors such as the Mi-1 gene in tomatoes, which encodes a nucleotide-binding leucine-rich repeat protein conferring resistance to several Meloidogyne species by recognizing nematode effectors and triggering defense responses.^[49]^[51] Weeds within the Solanaceae family, such as black nightshade (Solanum nigrum), act as alternative hosts, sustaining nematode populations between crop cycles and complicating management.^[52] In contrast, plants in the Brassicaceae family, like mustard (Sinapis alba), are typically non-hosts due to allelopathic compounds such as isothiocyanates released upon tissue disruption, which suppress nematode survival and enable effective crop rotation strategies to reduce soil infestations.^[53]

Symptoms and damage mechanisms

Root-knot nematodes (Meloidogyne spp.) induce a range of above-ground symptoms in infected plants that often mimic those of abiotic stresses such as drought or nutrient deficiency. These include stunted growth, wilting, and yellowing of leaves (chlorosis), which result from the nematodes' interference with root function and overall plant vigor.^[54] In severe infections, these symptoms can lead to yield reductions of up to 50% in susceptible crops like tomatoes and cotton, as the plant's ability to photosynthesize and translocate resources is compromised.^[55]^[25] Below-ground, the most distinctive signs are the formation of small, bead-like galls or knots on roots, typically 1-10 mm in diameter, caused by the hypertrophy of infected tissues around nematode feeding sites. These galls, along with swollen root nodules and cortical cracking, disrupt root architecture and expose tissues to environmental stresses.^[54]^[56] Cortical cracking in particular facilitates secondary infections by soilborne pathogens, exacerbating damage in crops such as sweetpotatoes.^[57] At the physiological level, root-knot nematodes establish syncytia—multinucleate feeding cells in the root cortex—that act as nutrient sinks, diverting carbohydrates, amino acids, and water from the plant to support nematode development. This process impairs the plant's uptake and transport of essential nutrients and water, leading to systemic stress.^[58] Additionally, nematodes secrete effector proteins that manipulate host hormone pathways, such as auxin and jasmonic acid signaling, promoting abnormal cell proliferation for gall formation while suppressing plant defense responses.^[54]^[25] Interactions with other pathogens amplify damage through synergism, where nematode-induced wounds and immune suppression create entry points for secondary invaders. For instance, co-infection with Fusarium oxysporum in tomatoes results in a disease complex more severe than either pathogen alone, with enhanced vascular wilt and root decay due to combined disruption of water conductance.^[25] Similarly, root-knot nematodes increase susceptibility to bacterial pathogens like Ralstonia solanacearum by altering root exudates and defense signaling.^[59]

Economic and environmental impact

Agricultural losses and affected crops

Plant-parasitic nematodes, including root-knot species (Meloidogyne spp.), inflict substantial economic damage on global agriculture, with estimated annual losses exceeding $100 billion worldwide due to reduced crop yields and quality.^[60] These losses stem primarily from the nematodes' interference with root function, leading to stunted growth and diminished nutrient uptake in infected plants. Yield reductions due to plant-parasitic nematodes, including root-knot species, average 10-14% globally across major crops, with higher losses (up to 20%) possible in vegetables under intensive farming and high nematode densities.^[61] Among the most severely affected crops are those in the Solanaceae family, such as tomatoes and potatoes, where losses can reach up to 30% in susceptible varieties under heavy infestation.^[62] Cucurbitaceae crops like cucumbers and Fabaceae legumes including soybeans also suffer significant impacts, with gall formation disrupting vascular tissues and resulting in 15-25% yield declines in field conditions. Fiber crops such as cotton experience similar setbacks, with root-knot nematodes contributing to 5-10% annual losses in cotton-growing regions, compounding effects from other pests.^[63] The burden is disproportionately borne in tropical and subtropical regions, where warm soils favor nematode reproduction and survival, accounting for the majority of global cases—particularly in developing countries reliant on smallholder farming of high-value crops. In the United States, plant-parasitic nematodes, including root-knot species, cause approximately $8 billion in annual losses, mainly in vegetable and row crops across southern states.^[61] Historically, major outbreaks were first documented in the late 19th century on sugarcane in Java, leading to the identification of Meloidogyne javanica and highlighting the nematodes' threat to tropical agriculture. Recent trends indicate escalating impacts, with climate warming expanding suitable habitats northward and intensifying infestation severity in established areas.^[64]^[65] Beyond direct economic costs, root-knot nematodes have environmental implications by disrupting soil health and biodiversity; their feeding reduces root biomass, affecting soil structure and carbon sequestration, while management practices like chemical nematicides can lead to groundwater contamination and harm to non-target soil organisms.^[66]

Distribution and epidemiology

Root-knot nematodes (Meloidogyne spp.) exhibit a cosmopolitan distribution, with the highest population densities occurring in warm, tropical, and subtropical climates across Africa, Asia, South America, and parts of North America, particularly between latitudes 30°S and 30°N.^[67] These nematodes thrive in regions with suitable soil temperatures and moisture, leading to greater prevalence in agricultural areas of these continents. In contrast, M. hapla, known as the northern root-knot nematode, predominates in cooler temperate zones, including parts of North America, Europe, and Asia, where it adapts to lower temperatures that limit other species.^[68] Globally, species such as M. incognita and M. javanica are the most widespread and economically significant, infecting a broad range of crops in diverse agroecosystems.^[69] The primary mechanisms of spread for root-knot nematodes involve short-distance dissemination through contaminated soil, infected seedlings, irrigation water, and farm equipment, which facilitate local infestation in fields.^[1] Long-distance transport occurs mainly via international trade, including the movement of infested plant material such as roots and soil, which has contributed to their global proliferation since the early 20th century.^[70] Human activities, including the trade of ornamental plants and agricultural commodities, have accelerated this dissemination, allowing nematodes to establish in new regions beyond their native ranges.^[71] Population dynamics of root-knot nematodes are characterized by density-dependent reproduction, where initial soil densities influence multiplication rates and subsequent damage potential.^[72] Damage thresholds typically range from 1 to 10 second-stage juveniles (J2) per gram of soil, beyond which significant host plant impairment occurs, varying by species, host, and environmental conditions.^[73] Modeling of these dynamics often incorporates temperature-based degree-day accumulations, using a base temperature of 10°C to predict developmental stages and generation times, such as approximately 500 degree-days for M. hapla to complete a life cycle.^[74] Emerging challenges include the expansion of root-knot nematode ranges due to climate change, with warmer temperatures projected to enable northward shifts into temperate regions by 2050, potentially increasing infestation risks in previously unaffected areas.^[75] In response, quarantine regulations, such as those outlined in EU Council Directive 2000/29/EC and subsequent updates under Regulation (EU) 2019/2072, designate certain species like M. chitwoodi, M. fallax, and M. enterolobii as Union quarantine pests to prevent introduction via traded plants and soil.^[76] These measures aim to mitigate transboundary spread amid evolving climatic pressures.^[77]

Management strategies

Cultural and physical controls

Cultural and physical controls for root-knot nematodes (Meloidogyne spp.) emphasize non-chemical practices that disrupt the nematodes' life cycle, reduce population densities, and enhance soil health without relying on synthetic inputs. These methods are particularly valuable in organic farming and integrated pest management systems, where they can suppress nematode numbers by 50-90% depending on implementation and local conditions.^[78]^[79] Crop rotation is a foundational strategy, involving the alternation of susceptible host crops, such as tomatoes or other solanaceous plants, with non-host species like cereals (e.g., corn, wheat, or small grains) for at least one to three years to starve juvenile nematodes of suitable roots. Grasses and grains serve as poor hosts, preventing reproduction and allowing natural die-off, with studies showing population reductions of up to 80% after two seasons.^[78]^[80] Incorporating marigolds (Tagetes spp.) into rotations provides additional suppression through root-exuded allelochemicals like alpha-terthienyl, which are nematicidal and can inhibit egg hatching and juvenile motility, achieving 60-90% control when grown as a cover crop for 8-12 weeks.^[81]^[82] Soil solarization, a physical method, uses transparent polyethylene sheeting (1-2 mil thick) to trap solar radiation and heat moist, tilled soil during the hottest summer months, typically for 4-6 weeks, raising temperatures to 40-55°C (104-131°F) in the top 20-30 cm layer to kill 85-95% of second-stage juveniles (J2) and eggs. This technique is most effective in regions with high sunlight, such as the southern U.S., and can also reduce weed seeds and soilborne pathogens, with peak efficacy when soil moisture is maintained at 50-75% field capacity prior to covering.^[83]^[84]^[85] In areas prone to water management, alternate flooding and drainage cycles can similarly suppress populations by creating anaerobic conditions lethal to nematodes, though this is less common and best suited to low-lying fields, reducing densities by 70% over multiple cycles in rice or vegetable rotations.^[86] Sanitation practices prevent the spread of nematodes via contaminated materials, including the prompt removal and destruction (by burning or deep burial) of infected roots and plant debris after harvest to eliminate egg masses and J2. Cleaning tools, equipment, and footwear with water or disinfectants before moving between fields avoids mechanical transmission, while vigilant weed control targets alternative hosts like nightshade or purslane that harbor nematodes. Fallowing infested areas—leaving soil bare and weed-free for 6-12 months—starves populations, with one-year fallows lowering root-knot densities by 70-90% through the absence of host roots, often enhanced by shallow tillage to expose nematodes to desiccation.^[80]^[87]^[88] Organic amendments, such as compost or animal manures incorporated at 10-20 tons per hectare, boost soil microbial activity and structure, indirectly suppressing nematodes by promoting antagonistic bacteria and improving plant vigor to tolerate low-level infestations. Green manures from brassica crops like mustard (Brassica juncea) offer direct control through biofumigation: when tilled into soil at flowering, they release isothiocyanates—natural fumigants toxic to J2 and eggs—achieving 60-80% reduction in populations, comparable to mild chemical treatments in field trials. These amendments are most effective when chopped and incorporated fresh, followed by tarping to trap volatiles for 2-4 weeks.^[89]^[90]^[91]

Chemical and biological controls

Chemical nematicides remain a primary tool for managing root-knot nematodes, though their use is increasingly regulated due to environmental and health concerns. Fumigant nematicides, such as methyl bromide, were historically effective against species like Meloidogyne incognita but were phased out in the United States by January 1, 2005, under the Montreal Protocol and Clean Air Act due to ozone depletion risks.^[92] Alternatives like 1,3-dichloropropene (Telone II) provide comparable control by diffusing through soil to target nematode juveniles and eggs, reducing root-knot populations by up to 90% in field trials on crops such as tomatoes and cucumbers when applied pre-planting under tarps.^[93] Non-fumigant options, including the carbamate oxamyl (Vydate), inhibit nematode acetylcholinesterase, achieving 70-90% suppression of egg production and juvenile penetration in greenhouse and field settings, though efficacy diminishes after 48-56 days and poses groundwater contamination risks.^[94] Biological controls offer sustainable alternatives by leveraging natural antagonists to suppress root-knot nematodes without broad-spectrum toxicity. The bacterium Pasteuria penetrans acts as an obligate hyperparasite, with its endospores attaching to the cuticle of second-stage juveniles (J2) of M. incognita, preventing host invasion and reducing populations by 50-80% in microplot studies when applied at rates of 100,000 endospores per gram of soil.^[95] Fungi such as Paecilomyces lilacinus (strain 251) parasitize nematode eggs, degrading egg masses and limiting hatching by up to 70% in tomato roots, as demonstrated in controlled inoculations.^[96] Predatory nematodes like Pristionchus species contribute by feeding on free-living stages, with strains such as P. entomophagus showing potential to disrupt J2 motility in soil assays, though field efficacy varies with environmental conditions.^[97] Application methods for both chemical and biological agents emphasize timing and delivery to target vulnerable life stages, particularly J2 during pre-planting. Soil drenches with oxamyl or P. penetrans suspensions ensure root-zone penetration, while seed treatments using non-fumigants like fluensulfone extend protection through seedling emergence.^[98] However, resistance challenges persist, as M. incognita populations have developed tolerance to carbamates like oxamyl through enhanced detoxification enzymes, reducing control efficacy in repeated applications.^[99] Recent advances include RNA interference (RNAi)-based sprays, which deliver double-stranded RNA targeting essential nematode genes such as those for parasitism, achieving 60-89% reduction in M. incognita reproduction in post-2020 field trials on tomatoes via topical application.^[100] Biopesticides like Bacillus firmus strain I-1582 have gained regulatory approval (e.g., EPA registration) for systemic nematicidal activity, colonizing roots to impair J2 attachment and suppress galling by 40-70% in diverse crops.^[101] These innovations prioritize specificity and reduced environmental impact over traditional broad-use chemicals.

Host resistance and integrated approaches

Host resistance to root-knot nematodes (Meloidogyne spp.) primarily involves genetic mechanisms that prevent successful parasitism, with the most studied being the hypersensitive response (HR) triggered by dominant resistance genes. In tomato (Solanum lycopersicum), the Mi-1 gene, derived from wild tomato species such as Solanum peruvianum, encodes a coiled-coil nucleotide-binding leucine-rich repeat (CC-NB-LRR) protein that confers resistance to three key species: M. incognita, M. javanica, and M. arenaria.^[102]^[103] This response involves rapid cell death at the infection site, limiting nematode feeding and reproduction through localized necrosis and the accumulation of reactive oxygen species and salicylic acid signaling.^[104]^[105] Polygenic quantitative resistance, in contrast, operates through multiple quantitative trait loci (QTLs) that collectively reduce nematode reproduction and galling without a pronounced HR, as identified in crops like soybean and cowpea where major QTLs explain partial resistance effects.^[57]^[106] Plant breeding efforts have focused on introgressing these resistance genes into commercial cultivars to provide durable protection. The Mi-1 gene was first incorporated into tomato varieties in the 1940s from wild relatives, leading to widespread adoption in processing tomatoes by the 1970s, where it effectively controls reproduction of avirulent nematode populations.^[107]^[108] However, challenges arise from the evolution of virulent nematode strains, such as M. enterolobii, which overcome Mi-1-mediated resistance by evading recognition, necessitating the stacking of multiple Mi genes (e.g., Mi-1 with Mi-9) or exploration of novel sources to maintain efficacy.^[107]^[109] Integrated pest management (IPM) for root-knot nematodes emphasizes combining host resistance with other tactics to minimize reliance on any single method and delay virulence evolution. This approach involves economic threshold-based decisions, such as monitoring second-stage juvenile (J2) densities via bioassays to guide interventions, alongside crop rotation with non-host plants and judicious use of certified resistant cultivars.^[78]^[79] For instance, in tomato production, IPM programs integrate Mi-1-resistant varieties with soil sampling to achieve population reductions of over 90% in low-infestation fields, enhancing sustainability while addressing variable field pressures.^[78] Future directions in host resistance include gene editing technologies like CRISPR/Cas9 to engineer broad-spectrum resistance by targeting susceptibility genes or enhancing R-genes. In the 2020s, trials have demonstrated success in rice by mutating the OsHPP04 susceptibility gene, reducing nematode galling by up to 70%, and in tomato through editing for stable, non-transgenic lines with improved Mi-1 durability.^[110]^[111] Similarly, CRISPR applications in sweetpotato have introduced resistance from guava sources without altering key agronomic traits, paving the way for sensor-based early detection systems to integrate with edited varieties for proactive management.^[112]

References

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Root-Knot Nematode - Wisconsin Horticulture
Mar 1, 2024 · Root-knot nematodes (Meloidogyne spp.) are small, soilborne, worm-like organisms that infect many agricultural and horticultural plants.Missing: scientific | Show results with:scientific
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Management of Root-Knot Nematodes in Bedding Plants
Jun 22, 2020 · Root-knot nematodes (Meloidogyne spp.) (Figure 1) are the most important and most economically devastating nematodes on ornamentals.
[3]
[PDF] Root-knot Nematode - Center for Crop Diversification
Root-knot nematodes (Meloidogyne spp.) are plant- parasi c roundworms that result in decreased plant vigor and yield followed by eventual plant death These.
[4]
[PDF] Identification of Root-knot Nematodes (Meloidogyne spp.) of ...
Root-knot nematodes are vermiform at the early stages of their life cycles and females have an enlarged body. These soil borne plant parasites got their ...
[5]
None
### Economic Importance and Global Impact of Root-Knot Nematodes (Meloidogyne spp.)
[6]
Meloidogyne - Nemaplex - UC Davis
Sep 22, 2025 · Classification: Rhabditida · Tylenchina · Tylenchoidea · Meloidogynidae · Meloidogyninae. Meloidogyne Goeldi, 1892.
[7]
Meloidogyne spp. (Root-knot nematodes) - Tomato - Ephytia
The genus Meloidogyne comprises about a hundred species: they are extremely polyphagous sedentary endoparasites which attack cultivated Solanaceae, very ...
[8]
Meloidogyne incognita - SweetARMOR
Meloidogyne incognita (southern root-knot nematode) was originally described in 1919 by Kofoid and White, with the specific epithet reflecting the unknown or ...
[9]
(PDF) Literature review of Meloidogyne javancia - ResearchGate
Oct 28, 2021 · Meloidogyne javanica (Trueb, 1885) Chitwood, 1949 is commonly known as the Root-Knot nematode. It is a highly destructive, polyphagous plant ...
[10]
Meloidogyne hapla, the Northern Root-Knot Nematode, in Florida ...
Apr 1, 2019 · The NRKN was first described from a potato field in Long Island, New York (Chitwood 1949). This parthenogenetic (asexually reproducing) ...Biology And Ecology · Hosts/species Affected · Damage Symptoms
[11]
Molecular characterization of root-knot nematodes (Meloidogyne ...
Oct 30, 2019 · Meloidogyne incognita is the most abundant species that was identified in 95% samples and was the only species in field crops including soybean ...
[12]
Molecular diagnostic key for identification of single juveniles of ...
Sep 22, 2006 · A molecular protocol is presented for distinguishing seven of the most common and economically important Meloidogyne spp.Missing: criteria | Show results with:criteria
[13]
THE INTERNATIONAL MELOIDOGYNE PROJECT-ITS GOALS AND ...
The root-knot nematodes (Meloidogyne species) are likely to be found wherev er plants grow. They have been collected from frozen soil, tropical rain forests ...
[14]
Diversity and evolution of root-knot nematodes, genus Meloidogyne
Molecular phylogenetic studies have highlighted divergence between mitotic and meiotic parthenogenetic RKN species and probable interspecific hybridization.
[15]
Early development of the root-knot nematode Meloidogyne incognita
Apr 28, 2016 · The root-knot nematode Meloidogyne incognita (Tylenchida) is an economically important plant parasite with a wide host range, and abundant field ...
[16]
https://brill.com/previewpdf/journals/nema/40/1-4/article-p150_10.xml
[17]
None
Summary of each segment:
[18]
Biology, Taxonomy, and Management of the Root‐Knot Nematode ...
Jun 25, 2021 · This review paper is focused on the sweet potato M. incognita biology, taxonomy, geographical distribution, and management measures.Abstract · Introduction · Biology and Taxonomy · Management Measures
[19]
Root-Knot Nematode of Tomato - NC State Extension Publications
Aug 16, 2020 · ... eggs per female (Figure 3), depending on the species. However, when ... *M.a = Meloidogyne arenaria; M.e = M. enterolobii; M.i = M ...
[20]
Thermal requirements for the embryonic development and life cycle ...
Jan 19, 2012 · The optimal temperatures for egg development in both species were 25 and 30°C. At the temperature range 15–30°C, the egg developmental phases ...
[21]
Temperature Effects on Development of Meloidogyne Enterolobii ...
Developmental time from infective J2 to reproductive stage for the three species was shorter at 30°C than at 25°C, and 30°C to 25°C. The shortest time and DD to ...
[22]
Hatching mechanisms of nematodes | Parasitology | Cambridge Core
Nov 8, 2011 · Investigations of nematode hatching have concentrated on the parasitic forms, especially on synchronization between host and parasite life-cycles.
[23]
Effects of Tomato Root Exudates on Meloidogyne incognita
Apr 29, 2016 · The effects of the root exudates on Meloidogyne incognita (M. incognita) egg hatch, survival and chemotaxis of second-stage juveniles (J2) were ...
[24]
Hatching of parasitic nematode eggs: a crucial step determining ...
In Meloidogyne incognita, extracts from tomato roots have been shown to supress hatching [61]. The level of hatching suppression is dependent on the tomato ...
[25]
role of plant growth promoting bacteria and abiotic factors - Frontiers
6.1 Soil temperature. Temperature influences nematode behavior such as egg hatching, nematode movement, root infection, their development and existence in ...
[26]
[PDF] MANAGEMENT OF ROOT-KNOT NEMATODE IN VEGETABLE ...
Their movement and therefore their efficacy is influenced by factors such as soil temperature, moisture, texture and the amount of un-decomposed organic matter.<|separator|>
[27]
MIGRATION AND REPRODUCTION OF MELOIDOGYNE ...
Dec 1, 2016 · This is the first report of using a three dimensional environment to monitor the distance M. incognita J2 migrate to a host root system, as ...
[28]
Screening of chemical attractants for second‐stage juveniles of ...
Dec 28, 2020 · To date, CO2 is the most recognized attractant for root-knot nematodes and some other plant-parasitic nematodes (Curtis et al., 2009; Pline & ...
[29]
Chemotaxis can take plant-parasitic nematodes to the source of a ...
Oct 29, 2010 · Prolonging the migratory phase of nematodes in soil will reduce their infectivity and increase their exposure to natural enemies.
[30]
[PDF] Parasitic Nematodes in Alfalfa
Root-knot nematode populations are likely to decrease 80–90 percent within a year in fallow soil. However, until host roots or crowns and stems from a previous ...
[31]
[PDF] RESEARCH/INVESTIGACIÓN MIGRATION AND REPRODUCTION ...
Second-stage juveniles placed in the center of each cylinder would need to move either 5 or 10 cm and pass through a root- preventing mesh to reach tomato roots ...
[32]
The effect of soil water flow and soil properties on the motility of ...
The movement of root-knot nematode Meloidogyne incognita second-stage juveniles was investigated in a column filled with two types of fillings, ...<|control11|><|separator|>
[33]
Vertical Migration of Second-stage Juveniles of Meloidogyne ... - NIH
Apr 22, 2024 · M. enterolobii J2 migrate over 13 cm vertically in 3 days, even without plants. Migration is greater at 26ºC, with 60% at >13cm at 12 days.
[34]
Creation and Validation of a Temperature-Based Phenology Model ...
The base temperature (Tb) and the thermal constant (S) from nematode inoculation to females starting to lay eggs were 11.3 °C and 323 accumulated degree days ( ...
[35]
Effect of water flow on the mobility of the root-knot nematode ...
The role of soil pores that enable root-knot nematode (Meloidogyne incognita) second stage juveniles (J2) to escape rapid water flow in soil was demonstrated.
[36]
Bio-Organic Management of Root-Knot Nematodes in Sustainable ...
Mar 19, 2025 · Using their stylet, they inject a mixture of hydrolytic enzymes such as endoglucanase, endoxylnase, pectatelase, amylase, cellulase, and ...
[37]
Root-knot nematode - American Phytopathological Society
Jan 1, 2003 · Until Chitwood's work in 1949, which defined 4 species and one subspecies (M. incognita acrita) within the genus Meloidogyne, the root-knot ...Missing: hierarchy | Show results with:hierarchy<|control11|><|separator|>
[38]
Root-Knot Nematodes Exhibit Strain-Specific Clumping Behavior ...
The J2 typically enters the host root in the zone of elongation then migrates to the vascular cylinder to establish a feeding site and complete its life cycle.<|control11|><|separator|>
[39]
Pattern‐triggered immunity against root‐knot nematode infection - NIH
The J2 enters the root at the zone of elongation, travels down to the root ... feeding site (Figure 1D). The J2 uses its stylet to secrete effectors ...
[40]
Endo-β-1,4-Glucanase Expression in Compatible Plant–Nematode ...
The giant cells of root-knot nematodes are formed by repeated karyokinesis uncoupled from cytokinesis, whereas the syncytia formed by cyst nematodes arise from ...
[41]
Overview of Root-Knot Nematodes and Giant Cells - ScienceDirect
RKNs interfere with the genetic programmes of their hosts to transform root vascular cells into giant cells (GCs) through the injection of nematode effectors ...
[42]
Meloidogyne arenaria - Nemaplex
Dec 16, 2024 · Meloidogyne arenaria, or Peanut Root-knot Nematode, is a sedentary endoparasite identified by cuticular markings, with peanut as its type host.<|separator|>
[43]
Early development of the root-knot nematode Meloidogyne incognita
Apr 28, 2016 · The optimal temperature for development of M. incognita is 28 °C, and corresponds to the geographical distribution of this nematode in ...
[44]
Phytoparasitic Nematode Control of Plant Hormone Pathways - PMC
(2015) found that, due to a higher cytokinin/auxin ratio, syncytia resemble shoot-forming calli and galls are similar to solid callus. However, it is still ...
[45]
Genetic variability and adaptive evolution in parthenogenetic root ...
Jan 11, 2006 · This review focuses on those RKN species that reproduce exclusively by mitotic parthenogenesis (apomixis), in contrast to those that have meiotic/amphimitic ...
[46]
Meiotic Parthenogenesis in a Root-Knot Nematode Results in ... - NIH
Many isolates of the plant-parasitic nematode Meloidogyne hapla reproduce by facultative meiotic parthenogenesis. Sexual crosses can occur.
[47]
Diversity and Evolution of Root-Knot Nematodes, Genus Meloidogyne
May 13, 2013 · Evolution of AT-rich mitochondrial DNA of the root-knot nematode ... Sequence and genetic map of Meloidogyne hapla: a compact nematode genome for ...Missing: polyphyletic | Show results with:polyphyletic
[48]
Genetic tests of ancient asexuality in Root Knot Nematodes reveal ...
Jul 7, 2008 · The data strongly suggests the tropical root knot nematode apomicts have a recent origin and are not anciently asexual. The results support that ...
[49]
Combating Root-Knot Nematodes (Meloidogyne spp.) - MDPI
In particular, the root-knot nematodes (RKN), Meloidogyne spp., exhibit a broad host range, threatening more than 2000 plants, including both monocotyledon and ...
[50]
Msp40 effector of root-knot nematode manipulates plant immunity to ...
Jan 22, 2016 · Root-knot nematodes (RKNs) are obligate biotrophic parasites that invade plant roots and engage in prolonged and intimate relationships with ...
[51]
Tomato Natural Resistance Genes in Controlling the Root-Knot ...
Nov 14, 2019 · These genes that confer resistance to the RKN have already been identified as Mi-1, Mi-2, Mi-3, Mi-4, Mi-5, Mi-6, Mi-7, Mi-8, Mi-9, and Mi-HT.
[52]
[PDF] Weed Hosts of Root-Knot Nematodes Common to Florida1
Weed Hosts of Root-Knot Nematodes Common to Florida ... Nightshade. Solanaceae. Ma, Mi. Solanum americanum. American black nightshade. Solanaceae. Mi, Mj, Mm. S.
[53]
Evaluation of 31 Potential Biofumigant Brassicaceous Plants ... - NIH
Brassicaceous cover crops can be used for biofumigation after soil incorporation of the mowed crop. This strategy can be used to manage root-knot nematodes ...
[54]
Combating Root-Knot Nematodes (Meloidogyne spp.) - NIH
The development of giant cells involves sequential mitoses without cytokinesis, resulting in an increased number of nuclei and cell size [7]. Pattern-triggered ...Missing: timeline | Show results with:timeline
[55]
[PDF] MU Guide - MU Extension - University of Missouri
Fortunately, only a few fields had enough root-knot nematodes to cut yield. ... However, losses may exceed 50 percent of the yield potential in severely.
[56]
Root-Knot Density as a New Index Can Quantitatively Diagnose the ...
Symptoms of root-knot nematode disease damage include: (1) leaf chlorosis, plant stunting, slow growth, abnormal wilting and premature senescence in ...
[57]
Discovery of a major QTL for root-knot nematode (Meloidogyne ...
The cracking and secondary infections reduces the market value of the sweetpotato storage roots by directly affecting their quality. RKNs are obligatory ...
[58]
Effectors of Root-Knot Nematodes: An Arsenal for Successful ...
A Meloidogyne incognita effector MiISE5 suppresses programmed cell death to promote parasitism in host plant. Sci. Rep. 8, 1–12. doi: 10.1038/s41598-018 ...
[59]
Root-Knot Nematode - an overview | ScienceDirect Topics
Root-knot nematodes (Meloidogyne spp.) are plant-parasitic nematodes that invade the roots of various plants, causing the formation of galls that lead to ...<|control11|><|separator|>
[60]
Determining the damage threshold of root-knot nematode ...
The annual crop loss of around 100 billion US dollars across the world is because of root knot nematode, Meloidogyne spp. (Brand et al., 2010). The annual crop ...Missing: 2020s | Show results with:2020s
[61]
Variability, Adaptation and Management of Nematodes Impacting ...
In economic terms, these estimated annual crop losses translate to at least $8 billion in the United States and $80 - $157 billion worldwide [4-6]. These ...Missing: 2020s | Show results with:2020s
[62]
Effect of botanicals, organic nutrient sources, and bio-control agents ...
Sep 12, 2025 · Root-knot nematode (Meloidogyne incognita) causes up to 30% yield loss in tomato (Solanum lycopersicum) worldwide, and reliance on synthetic ...
[63]
Cotton Disease Loss Estimates from the United States — 2023
Feb 16, 2024 · The top yield reducing disease or disease group in 2023 was root-knot nematodes, followed by reniform nematode, seedling diseases, Stemphylium ...
[64]
https://brill.com/downloadpdf/display/title/37960.pdf
[65]
Global Warming - NEM-EMERGE
Global warming is behind the northward expansion of nematodes historically classified as tropical species, with new root-knot nematode and potato cyst ...
[66]
Potential global distribution of the guava root-knot nematode ...
In contrast to the current distribution, by 2090, the total suitable area will increase by 18.57%, and the highly suitable area will increase by 4.11%. In that ...
[67]
Nematodes / Lettuce / Agriculture: Pest Management ... - UC IPM
Meloidogyne hapla, the northern root knot nematode, generally occurs in cooler regions than the other three Meloidogyne species that prefer hot summer climates.
[68]
Root-knot nematode assessment: species identification, distribution ...
Aug 7, 2023 · Meloidogyne incognita and M. javanica were found to be the most prevalent species in the different regions followed by M. arenaria and M. hapla.Abstract · Introduction · Materials and methods · Results and discussion
[69]
[PDF] Worldwide Dissemination and Importance of the Root-knot ...
Abstract: Root-knot nematodes are widely distributed throughout the world. Their distribution and economic importance are purported to be related to ...<|separator|>
[70]
Plant Parasitic Nematodes - USDA ARS
Mar 9, 2024 · Nematodes are microscopic worms that cause eighty billion dollars of crop loss in the world each year. All crops are damaged by at least one species of ...Missing: via international trade
[71]
Modelling nematode population growth and damage - ScienceDirect
Damage by RKN was shown to be density dependent, to increase with increasing duration of plant growth, and to be much greater for good than poor hosts. PCN ...
[72]
Damage Threshold and Population Dynamic of Meloidogyne ...
Jan 7, 2025 · Studies across various crops and regions have suggested that the damage threshold for Meloidogyne spp. ranges from 0.10 to 2 nematodes per gram ...
[73]
[PDF] Download - WSU Research Exchange
hapla can complete a single life-cycle after about 500 degree-days (base 10 C). ... Development of the root-knot nematode as affected by temperature.
[74]
The pervasive impact of global climate change on plant-nematode ...
In India, climate change is influencing the geographic distribution pattern of rice root-knot nematode M. graminicola. Earlier considered to be a pest of upland ...
[75]
[PDF] quarantine nematodes – european legislation, current status and ...
Council. Directive 2000/29/EC (consolidating the old and much amended Directive 77/93/EEC) is the principal piece of legislation governing plant health ...
[76]
EU Quarantine Pests: Nematodes - EURL
Dec 14, 2019 · Article 5(2) of this Plant Health Law calls for the establishment of a “list of Union quarantine pests”. This list was established and is ...
[77]
Root-Knot Nematode / Tomato / Agriculture - UC IPM
The number of root-knot juveniles in soil samples can be a reliable guide to potential yield loss in processing tomatoes; below a certain level, the population ...
[78]
Nematode Management in Tomatoes, Peppers, and Eggplant
Most vegetable crops produced in Florida are susceptible to nematode injury, particular by root-knot and sting nematodes. Plant symptoms and yield ...
[79]
Control of Root-Knot Nematodes in the Home Vegetable Garden
Nematode-control measures will significantly reduce root-knot and other nematodes from the garden site. The continued combined use of rotation, resistance ...
[80]
ENY063/IN892: Cover Crops for Managing Root-Knot Nematodes
Jan 3, 2023 · Marigold can suppress 14 genera of plant-parasitic nematodes, especially root-knot nematodes and lesion nematodes (Pratylenchus spp.). Different ...
[81]
[PDF] Root-Knot Nematodes - NC Department of Agriculture
One alternative to using nematicides is to intercrop and rotate vegetables with marigolds. Most cultivars of African marigold (Tagetes erecta) and French ...
[82]
[PDF] Soil solarization for nematode control
Normally 4 to 6 weeks is an adequate period of solarization; however, it is essential to check the soil temperature to determine if temperatures lethal to ...
[83]
[PDF] Soil Solarization - UC Vegetable Research & Information Center
Solarization may also be a beneficial addition to an integrated nematode control system. For example, excellent control of root knot nematode. (Meloidogyne ...
[84]
Root-Knot Nematodes in the Vegetable Garden - Clemson HGIC
Aug 1, 2025 · Soil Solarization: Soil solarization is a nonchemical method where the soil is tilled and moistened, then transparent polyethylene dropcloths ...
[85]
Tare Soil Disinfestation from Cyst Nematodes Using Inundation - PMC
Inundation has been shown to control nematodes like root-knot nematodes (Meloidogyne spp.) [6] or stem nematodes (Ditylenchus dipsaci) [7]. In flooded peat ...
[86]
Nematode Control in the Home Garden | Mississippi State University ...
Marigolds give off a substance from their roots that is toxic to nematodes, making these flowers a valuable aid in nematode control when they are planted in ...Resistant Varieties · Marigolds · Combination Of Controls<|separator|>
[87]
Nematode Management Guidelines - UC IPM
Fallowing is the practice of leaving the soil bare for a period of time. Fallowing for 1 year will lower root knot nematode populations enough to successfully ...
[88]
Soil Organic Matter, Green Manures, and Cover Crops for Nematode ...
Organic amendments can both improve tolerance of the plant to nematodes and also reduce nematode populations. However, they cannot magically eliminate a severe ...Soil Organic Matter · Adding Organic Matter To... · Green Manures
[89]
Biofumigation cover crops: Enhancing soil health and combating pests
Biofumigation is an alternative to chemical fumigation of crops. Cover crops such as mustard, canola and oilseed radish control pests, disease and weeds.Biofumigation Cover Crops... · Cover-Crop Termination · Soil Health
[90]
[PDF] Mustard Seed Meal for Management of Root-knot Nematode and ...
Mustard seed meals can be amended into soil as fertilizers, for suppression of soilborne pests and pathogens (Brown and Morra,. 2005; Gigot et al., 2013; ...Missing: manures | Show results with:manures
[91]
Chemical Nematicides for Control of Plant-Parasitic Nematodes in ...
Nov 16, 2018 · However, research suggests that effective control of root-knot nematodes can be achieved when Vapam is combined with nonfumigant nematicides.
[92]
Managing root-knot nematodes and weeds with 1,3 ... - PubMed
Mar 23, 2011 · The present data support the conclusion that 1,3-D is a promising MeBr alternative for managing nematodes and weeds in cucumber crops and can be ...
[93]
Factors affecting the efficacy of non-fumigant nematicides ... - PubMed
Oxamyl also provided adequate nematode control for the first 48-56 days after its application, regardless of the application method used and its relatively ...
[94]
Review of Pasteuria penetrans: Biology, Ecology, and ... - NIH
Pasteuria penetrans is a mycelial, endospore-forming, bacterial parasite that has shown great potential as a biological control agent of root-knot nematodes.
[95]
Biological control of the root-knot nematode Meloidogyne incognita ...
The fungal biocontrol agent, Paecilomyces lilacinus strain 251 (PL251), was evaluated for its potential to control the root-knot nematode Meloidogyne incognita ...
[96]
Bacterial transfer from Pristionchus entomophagus nematodes ... - NIH
Jun 25, 2021 · The necromenic nematode Pristionchus entomophagus has been frequently found in nests of the invasive European ant Myrmica rubra in coastal Maine, United States.
[97]
How seed-applied nematicides work - Crop Protection Network
May 19, 2020 · Seed-applied nematicides create a "zone of protection" by moving from the seed coat to the soil and water, directly contacting nematodes to ...
[98]
Sublethal concentrations of conventional nematicides alter ... - Nature
Jan 5, 2023 · The two major nematicide categories used to manage M. incognita are carbamates and organophosphates. Both groups target the nematode nervous ...
[99]
Attempt to Silence Genes of the RNAi Pathways of the Root-Knot ...
Mar 23, 2020 · Attempt to silence drsh-1, mut-7, drh-3, rha-1, pash-1, and vig-1 through HIGS led to reduction in nematode infestation by up to 89%.
[100]
Bacillus firmus Strain I-1582, a Nematode Antagonist by Itself and ...
Jul 9, 2020 · Root-knot nematodes (RKN) belonging to the genus Meloidogyne are the most damaging PPN worldwide due to their wide range of plant hosts, ...
[101]
Mapping of the gene in tomato conferring resistance to root-knot ...
Oct 9, 2023 · The Mi-1.2 gene in tomato confers resistance to the Meloidogyne species M. incognita, M. arenaria and M. javanica, which are prevalent in tomato ...Introduction · Materials and methods · Results · Discussion
[102]
Mi-1-Mediated Resistance to Meloidogyne incognita in Tomato May ...
To date, Mi-1 is the only cloned R gene for RKN. In addition to RKN resistance, Mi-1 confers resistance to potato aphids, whiteflies and tomato psyllids [7], [8] ...
[103]
Salicylic Acid Is Part of the Mi-1-Mediated Defense Response to ...
These results indicate that SA is an important component of the signaling that leads to nematode resistance and the associated hypersensitive response.
[104]
Identification of genes involved in the tomato Mi-1-mediated ...
Jun 26, 2025 · 4. Discussion. Root-knot nematode (RKN) disease poses a significant global agricultural challenge, disrupting plant growth, nutrient uptake, ...Research Paper · 3. Results · 4. Discussion
[105]
A major quantitative trait locus resistant to southern root‐knot ...
Jan 6, 2021 · A major quantitative trait locus resistant to southern root-knot nematode sustains soybean yield under nematode pressure.
[106]
Tomato Natural Resistance Genes in Controlling the Root-Knot ...
M. enterolobii can break the resistance of the Mi-1 gene in tomatoes and peppers; this makes it difficult to control this type of root-knot nematode, ...
[107]
[PDF] Resistance to Plant-parasitic Nematodes: History, Current Use and ...
Even though it was several decades before root-knot resistant tomato cultivars were widely grown commercially, resistance condi- tioned by the Mi gene has been ...
[108]
Comparative histopathology of virulent and avirulent Meloidogyne ...
Aug 22, 2024 · The Mi-1.2 gene confers resistance to a wide range of Meloidogyne species, being the most important resistance factor employed in tomato breeding so far.
[109]
CRISPR/Cas9-mediated mutagenesis of the susceptibility gene ...
CRISPR/Cas9-mediated mutagenesis of the susceptibility gene OsHPP04 in rice confers enhanced resistance to rice root-knot nematode.
[110]
19A. Enhanced root-knot nematode resistance in tomato using ...
Furthermore, we used CRISPR/Cas9 technology to create stable non-GMO genome edited lines. We produced 6 independent gene- edited lines, in which gene mutations ...
[111]
Precision breeding the sweetpotato's future - Farm Progress
Oct 30, 2025 · “The goal is to edit in guava root knot nematode resistance without changing the quality, flavor, look, and response to other diseases in the ...