Fact-checked by Grok 2 weeks ago

Axon guidance

Axon guidance is the process by which extending axons from newly differentiated neurons navigate through complex environments to reach their synaptic targets, thereby establishing precise neural circuits during nervous system development.^[1] This navigation is primarily orchestrated by the growth cone, a specialized, motile structure at the axon tip that senses extracellular guidance cues and transduces them into cytoskeletal rearrangements to direct axonal extension, attraction, repulsion, or branching.^[1] Guidance cues include both diffusible signals, such as morphogens, and contact-dependent molecules embedded in the extracellular matrix or on cell surfaces, which elicit context-dependent responses in the growth cone.^[2] The core mechanisms of axon guidance involve receptor-ligand interactions that activate intracellular signaling pathways, regulating the dynamics of actin filaments and microtubules within the growth cone to control its motility and directionality.^[1] These pathways often converge on shared effectors, such as Rho GTPases, which modulate cytoskeletal assembly, while receptor trafficking, endocytosis, and endosomal signaling hubs fine-tune responsiveness to cues over time and space.^[1] Additional layers of regulation include alternative splicing of receptors, local protein synthesis from mRNAs transported into axons, and crosstalk between different guidance systems, enabling axons to integrate multiple signals at choice points during pathfinding.^[1] Invertebrate models, like Drosophila and C. elegans, have been instrumental in elucidating these conserved mechanisms, revealing how pioneer axons and glial cells also provide substrates for follower axons to track.^[3] Four major families of guidance molecules dominate axon pathfinding: netrins, which can attract or repel via receptors like DCC and UNC5; slits, acting as repellents through Robo receptors to midline crossing; semaphorins, often repulsive and signaling via plexins and neuropilins; and ephrins, which mediate bidirectional repulsive or attractive effects through Eph receptors.^[2] Complementary cues include morphogens such as Wnts and Sonic hedgehog (Shh), which provide long-range gradients, as well as cell adhesion molecules like integrins and FLRTs that stabilize interactions with the substrate.^[2] These molecules operate in a combinatorial code, with their expression patterns spatially and temporally restricted to guide axons along stereotyped trajectories in both central and peripheral nervous systems.^[1] Disruptions in axon guidance contribute to neurodevelopmental disorders, including autism spectrum disorders and corpus callosum agenesis, underscoring its critical role in wiring the brain.^[1] Beyond development, axon guidance mechanisms are implicated in neural regeneration, where reactivation of embryonic cues could promote repair after injury, as evidenced by studies in mammalian models showing their necessity for reconnecting regenerating axons to specific targets.^[4] Ongoing research continues to uncover how these systems achieve precision in vivo, integrating genetic, structural, and signaling insights to inform therapeutic strategies for neurological conditions.^[1]

Overview

Definition and Core Process

Axon guidance is the process by which extending axons navigate to specific targets within the nervous system, directed by extracellular cues that influence their growth and pathfinding.^[2] This navigation ensures the precise wiring of neural circuits essential for sensory, motor, and cognitive functions.^[5] The core process begins with axon outgrowth from the neuronal cell body, where the axon extends through polarized cytoskeletal dynamics, forming a specialized structure at its tip known as the growth cone.^[6] The growth cone senses environmental signals via surface receptors, transducing them into intracellular responses that steer directional changes, such as attraction or repulsion.^[7] Additionally, fasciculation occurs as growing axons bundle together, often adhering to pioneer axons or substrates, which stabilizes trajectories and facilitates collective navigation.^[7] Initial observations of axonal trajectories date to the late 19th century, when Santiago Ramón y Cajal described the directed growth of axons in developing nervous tissue, highlighting the role of growth cones in probing the environment.^[8] In 1963, Roger Sperry proposed the chemoaffinity hypothesis, suggesting that molecular tags on axons and targets enable specific recognition and orderly connections, laying the foundation for understanding guidance mechanisms.^[9] This process occurs primarily during embryonic development, when axons extend and form connections to establish neural circuitry, but similar guidance principles also operate in adult axon regeneration following injury.^[6]^[10]

Developmental and Functional Significance

Axon guidance plays a pivotal role in neural development by directing the extension and navigation of axons to form precise synaptic connections in the brain and spinal cord. This process establishes the foundational wiring of neural circuits, which is essential for integrating sensory inputs with motor outputs and supporting higher cognitive functions such as learning and memory. Disruptions in axon guidance during embryogenesis can lead to widespread connectivity deficits, underscoring its importance in orchestrating the complex architecture of the nervous system.^[11] The functional outcomes of successful axon guidance include the formation of major neural tracts that enable interhemispheric communication and specialized sensory processing. For instance, guidance cues direct the development of the corpus callosum, which connects the two cerebral hemispheres, and the optic nerve, which relays visual information from the retina to the brain. Errors in this process result in miswiring, as seen in achiasma, a congenital condition where retinal axons fail to cross properly at the optic chiasm, leading to uncrossed optic pathways and impaired binocular vision.^[12] Axon guidance mechanisms exhibit remarkable evolutionary conservation, with core signaling pathways shared between invertebrates, such as Drosophila, and vertebrates, indicating their ancient origins in metazoan nervous system development. These conserved elements, including ligand-receptor interactions for attraction and repulsion, ensure stereotyped wiring patterns across diverse species, from simple commissural pathways in flies to elaborate tracts in mammals. This conservation highlights the fundamental role of axon guidance in evolving increasingly complex neural architectures.^[13] In humans, the scale of axon guidance is immense, involving billions of axons that navigate distances up to 1 meter during gestation to reach distant targets and establish functional circuits. This precise navigation over vast scales is critical for the maturation of the 86 billion neurons in the adult brain, where even minor deviations can compromise organismal function.^[14]

Guidance Cues

Attractive Molecules

Attractive molecules in axon guidance are extracellular cues that promote the directed extension and turning of axons toward specific targets, primarily by activating receptors that trigger intracellular signaling cascades leading to cytoskeletal reorganization in the growth cone.^[1] Among these, netrins represent a primary class of attractive molecules, with netrin-1 in vertebrates and its homolog UNC-6 in C. elegans serving as prototypical examples. Netrin-1 binds to the receptor deleted in colorectal cancer (DCC) in vertebrates or its ortholog UNC-40 in C. elegans, initiating attraction through downstream activation of pathways that enhance actin polymerization and protrusion formation.^[15] A key mechanism involves the phosphoinositide 3-kinase (PI3K) pathway, where receptor activation recruits PI3K (AGE-1 in C. elegans), generating lipid second messengers that localize cytoskeletal regulators like MIG-10/lamellipodin to the plasma membrane, thereby promoting localized lamellipodia and filopodia extension toward the cue source.^[1] In vertebrates, netrin-1, secreted by floor plate cells in the embryonic spinal cord, guides commissural axons across the midline by forming a ventral-to-dorsal gradient that elicits chemoattraction.^[16] Genetic ablation of netrin-1 in mice disrupts this process, resulting in commissural axons failing to reach the midline, confirming its essential role in vivo.^[16] Similarly, in C. elegans, UNC-6 directs pioneer axons along the ventral nerve cord, with UNC-40 mediating attraction to maintain proper longitudinal trajectories.^[15] These examples illustrate how netrins function over short to intermediate distances, often integrating with other cues for precise navigation. Beyond netrins, neurotrophins such as brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) act as attractive cues by binding to tropomyosin receptor kinases (TrkB for BDNF and TrkC for NT-3), which elevate intracellular cyclic AMP (cAMP) levels to bias growth cone turning toward higher concentrations.^[17] This cAMP-dependent signaling modulates the response to gradients, converting potential repulsion into attraction and promoting axon branching and target innervation, as seen in trigeminal sensory axons drawn to maxillary process-derived BDNF and NT-3.^[18] In proprioceptive neurons, NT-3 specifically chemoattracts axons to muscle targets over short distances, enhancing survival and connectivity.^[19] Attractive guidance operates through multiple mechanisms, including chemoattraction via soluble gradients that diffuse over distances to orient growth cones, as demonstrated by netrin-1's diffusible action on commissural axons.^[1] Haptotaxis involves substrate-bound forms of these molecules, where axons adhere preferentially to increasing concentrations on extracellular matrices, facilitating directed migration; for instance, immobilized netrin-1 engages DCC to generate traction forces via integrin linkages.^[20] Short-range contact-mediated pull occurs when growth cones physically interact with cues, activating mechanotransduction pathways like focal adhesion kinase (FAK) to reinforce protrusion, as observed in netrin-1-induced adhesion and outgrowth.^[21] These modes allow attractive molecules to coordinate bidirectional guidance with repulsive cues for accurate pathfinding.

Repulsive Molecules

Repulsive molecules function as critical deterrents in axon guidance, inhibiting axonal extension into non-target regions by triggering growth cone collapse, repulsion, or turning away from inhibitory cues. These secreted or membrane-anchored proteins establish boundaries and channels for precise neural wiring during development, counterbalancing attractive signals to ensure axons reach appropriate targets. Unlike attractive cues that promote outgrowth, repulsive molecules induce rapid cytoskeletal disassembly, primarily through modulation of actin dynamics and Rho GTPase signaling.^[2] The main classes of repulsive molecules include Slits, semaphorins, and ephrins, each engaging specific receptor complexes to transduce inhibitory signals. Slits are large secreted glycoproteins that bind to Roundabout (Robo) family receptors on axonal growth cones, initiating repulsive responses via intracellular pathways that activate RhoA GTPase and promote actin depolymerization, leading to growth cone collapse. This interaction is evolutionarily conserved, as demonstrated in studies showing Slit-Robo binding directly mediates midline repulsion in both Drosophila and mammalian systems.^[22] Semaphorins, particularly class 3 members like Sema3A, exert repulsion through heterocomplexes of neuropilin-1 (Nrp1) and plexin-A receptors, which inhibit F-actin assembly and induce growth cone retraction by recruiting downstream effectors such as collapsin response mediator proteins (CRMPs). The formation of these Nrp1-plexin complexes is essential for Sema3A's chemorepellent activity on diverse axonal populations.^[23] Ephrins, as transmembrane ligands, bind Eph receptor tyrosine kinases to elicit bidirectional signaling; forward signaling in axons activates Eph receptors to drive repulsion through RhoA-dependent cytoskeletal contraction, while reverse signaling in target cells can promote adhesion or attraction, facilitating topographic mapping. This dual functionality arises from asymmetric kinase activity and endocytosis of Eph-ephrin complexes at contact sites.^[2] These repulsive cues operate through distinct mechanisms, including chemorepulsion via extracellular gradients for long-range guidance and contact-mediated repulsion for short-range boundary formation. Slits and soluble semaphorins like Sema3A diffuse to create inhibitory gradients that steer axons over distances, whereas membrane-bound ephrins induce localized collapse upon direct cell-cell contact, often amplified by endocytosis to sustain signaling. In the spinal cord, Slit2 secreted from midline structures prevents inappropriate recrossing of commissural axons post-midline traversal, ensuring unidirectional pathway formation by selectively repelling axons expressing high Robo levels after crossing.^[22] Similarly, in the retinotectal system, Sema3A expressed in the optic tectum channels retinal ganglion cell axons by eliciting stage-dependent collapse and turning, directing them away from ectopic zones to refine topographic projections.^[24] Ephrins contribute to short-range repulsion in mapping, where high ephrin gradients in posterior tectum collapse temporal retinal axons, preventing overshoot while reverse signaling in target neurons enhances adhesion for synapse stabilization.^[25]

Cellular and Structural Mechanisms

Growth Cone Dynamics

The growth cone, located at the distal tip of the extending axon, functions as the primary sensory and motile apparatus driving axon navigation during neural development. It is broadly divided into a central (C) domain, enriched with bundled microtubules and organelles, and a peripheral (P) domain, characterized by dynamic actin-rich structures that probe the extracellular environment. This organization allows the growth cone to integrate environmental cues and direct axonal pathfinding with high precision.^[26] The peripheral domain consists of lamellipodia and filopodia, which together form a veil-like or fan-shaped expanse for environmental sampling. Lamellipodia are broad, sheet-like protrusions composed of a branched meshwork of actin filaments, generated through dendritic nucleation and polymerization at the leading edge. Filopodia, in contrast, are slender, spike-like extensions (typically 5–10 μm long) supported by parallel bundles of 10–30 actin filaments, enabling fine-scale exploration of substrates and gradients. These actin-based protrusions extend and retract at rates of 0.1–1 μm/min, allowing the growth cone to detect adhesive or repulsive surfaces over distances of tens of micrometers.^[27]^[26] Growth cone motility cycles through distinct phases of protrusion, engorgement, consolidation, and retraction, orchestrated by coordinated cytoskeletal remodeling. Protrusion initiates the cycle, driven by rapid actin polymerization at filopodial and lamellipodial tips via the Arp2/3 complex, which nucleates branched filaments and counters retrograde actin flow (typically 1–7 μm/min) to extend new membrane. Engorgement follows, as myosin II contracts actin arcs to widen protrusions and facilitate cytoplasmic influx, creating corridors for microtubule invasion from the central domain. Consolidation advances microtubules into the stabilized periphery, bundling actin into stress fibers via myosin-mediated compression and forming a nascent axonal shaft, with advance rates up to 50 μm/hour in permissive environments. Retraction occurs upon repulsion, involving actin depolymerization and microtubule withdrawal, collapsing the growth cone to redirect or halt extension.^[28]^[26] As the sensory hub, the growth cone concentrates guidance cue receptors—such as those for netrins or semaphorins—at filopodial tips, enabling detection of extracellular gradients with submicrometer resolution. These receptors trigger localized calcium transients, brief elevations (100–500 nM) in intracellular Ca²⁺, often via influx through channels like TRPC1 or release from internal stores. Asymmetric calcium signaling guides turns: elevated transients on one side promote protrusion and microtubule stabilization toward attractants, while balanced or suppressed levels on the opposing flank induce retraction, ensuring precise steering without global collapse.^[29] In response to cues, growth cones exhibit adaptive behaviors that fine-tune navigation. Steering involves asymmetric advance, where enhanced protrusion and microtubule exploration on the cue-facing side (e.g., toward netrin-1 sources) reorients the axon by 30–90 degrees over minutes. Stalling arises from equilibrated attraction and repulsion, causing pauses (lasting 10–60 minutes) with collapsed filopodia and looped microtubules, as observed in balanced gradients. Branching facilitates target exploration, with side protrusions forming from stabilized actin foci, allowing axons to innervate multiple sites before selective pruning. These behaviors correlate with growth cone morphology—fan-like for steering, compact for stalling, and multi-veiled for branching—ensuring robust pathfinding across diverse terrains.^[30]^[27]

Signal Integration in Axons

Axons integrate multiple guidance cues through intracellular signaling pathways that converge to regulate growth cone behavior, enabling precise navigational decisions during development. This integration occurs primarily at the growth cone, where extracellular signals are transduced into coordinated cytoskeletal rearrangements.^[1] A key mechanism of crosstalk involves cyclic AMP (cAMP) levels, which modulate the response to guidance cues such as netrins, switching between attraction and repulsion. High intracellular cAMP concentrations convert netrin-induced repulsion to attraction by enhancing downstream signaling through DCC receptors, as demonstrated in Xenopus spinal neurons where elevation of cAMP via forskolin or analogs reversed MAG-induced repulsion. Low cAMP levels promote repulsion, illustrating how second messengers fine-tune cue interpretation based on the axon's developmental context. Pathway convergence often involves Rho family GTPases, which integrate attractive and repulsive signals via cytoskeletal control. Cdc42 activation promotes attraction by driving actin polymerization and filopodia protrusion, as seen in netrin-1 responses where it links DCC to N-WASP for growth cone advance.^[31] In contrast, RhoA mediates repulsion by activating ROCK to induce actomyosin contraction and growth cone collapse, particularly in response to semaphorins or ephrins.^[31] These GTPases converge on the cytoskeleton, balancing protrusion and retraction to determine net direction. Calcium ions serve as a critical second messenger in this process, with localized elevations triggering turns by asymmetrically regulating actin dynamics through effectors like CaMKII and calcineurin.^[29] For instance, high-amplitude calcium signals from netrin-1 influx promote attractive turns via enhanced filopodial extension, while lower amplitudes elicit repulsion.^[29] Axons employ threshold models for decision-making, where spatial and temporal summation of signals determines the net response only if a critical threshold is exceeded. In motor neurons, subthreshold concentrations of netrin-1 alone elicit minimal repulsion, but co-application with ephrin-B2 synergistically amplifies Src kinase activity, exceeding the threshold for robust turning via Unc5c-EphB complexes.^[32] This summation integrates cue gradients over time and space, ensuring directional bias only under sufficient stimulus strength.^[32] A representative example is midline crossing in the spinal cord, where balanced netrin attraction and slit repulsion allow axons to approach, cross, and then exit the midline. Netrin-1 via DCC draws commissural axons toward the floor plate, while slit via Robo provides post-crossing repulsion; their integration, modulated by Robo3 silencing of Robo1/2, prevents premature deflection and ensures timely exit. This dynamic balance highlights how opposing cues are temporally gated for accurate pathfinding.

Strategies for Tract Formation

Pioneer Axon Pathways

Pioneer axons represent the initial neurons to extend processes in a developing neural region, establishing foundational pathways that serve as scaffolds for subsequent axon tracts without depending heavily on diffuse, long-range guidance cues.^[33] These early outgrowths rely primarily on local environmental signals, such as contact-mediated interactions or short-range molecular attractants, to navigate and define reproducible routes across the nervous system.^[33] By laying down these pioneer pathways, they enable the efficient bundling and directed growth of later-arriving follower axons, thereby streamlining the formation of organized neural tracts during embryogenesis.^[33] The formation of pioneer axon pathways begins with the extension of growth cones from these neurons, which actively sense and respond to proximal cues in the extracellular matrix or on neighboring cells to advance directionally.^[34] As pioneers elongate and converge, they initiate bundling through fasciculation, a process mediated by cell adhesion molecules that promote axon-axon adhesion.^[35] A key example is L1-CAM, an immunoglobulin superfamily member that facilitates homophilic binding between axons, stabilizing bundles and allowing follower axons to adhere and grow along the established scaffold.^[36] This fasciculation not only reinforces the pathway but also amplifies guidance signals, as bundled axons can collectively respond to environmental cues more robustly than isolated pioneers.^[35] In vertebrates, retinal ganglion cell axons exemplify pioneer pathways by pioneering the optic tract; early uncrossed axons from the temporal retina lead the ingrowth into the tract, organizing its structure before ipsilateral crossed axons from the nasal retina join in a segregated manner.^[37] This sequential organization ensures proper topographic mapping from retina to brain targets. In Drosophila embryos, RP motorneurons (such as RP1, RP3, RP4, and RP5) function as pioneers, with their axons crossing the midline via the anterior commissure, then fasciculating posteriorly along the contralateral longitudinal connective before branching into peripheral nerves to innervate ventral muscles.^[34] These RP axons thus blaze trails that guide later motor axons, demonstrating conserved mechanisms across phyla. Despite their critical role, pioneer axons are often transient structures; in many systems, they may withdraw, degenerate, or become overshadowed by denser bundles of follower axons that stabilize the mature tract.^[33] This impermanence underscores their primary function as temporary guides rather than permanent circuit components.

Glial and Guidepost Roles

Glial cells play a crucial role in axon guidance by providing structural scaffolds, secreting molecular cues, and facilitating contact-mediated interactions that direct axonal trajectories during neural development. In the spinal cord, floor plate glia at the ventral midline secrete netrin-1, which forms a gradient to attract commissural axons toward the midline, enabling their crossing via interaction with the DCC receptor on growth cones.^[38] Although recent studies indicate that floor plate-derived netrin-1 may be dispensable in some contexts, with contributions from ventricular zone sources also essential, this secretion remains a foundational mechanism for midline navigation.^[39] Radial glia, extending from the ventricular zone to the pial surface, offer a permissive substrate for axonal migration, particularly in the cerebral cortex where callosal axons extend along radial glial fibers, with up to 64% of their surface in direct apposition to these scaffolds.^[40] Guidepost cells, often specialized neurons or glia positioned at key waypoints, provide discrete, contact-dependent cues to steer axons stepwise toward their targets. In the developing cerebral cortex, subplate cells function as transient guideposts for thalamocortical axons, forming temporary synapses that instruct proper layering and connectivity before the subplate is eliminated.^[41] Delta-Notch signaling mediates some of these interactions, where glial expression of the glycosyltransferase Fringe modulates Delta-Notch activation between neurons and glia, promoting subtype-specific glial differentiation and localized presentation of guidance cues like Slit proteins.^[42] Several mechanisms underpin these glial and guidepost functions. Channeling occurs through physical barriers formed by glial processes, which constrain axons to specific pathways, as seen in midline glial wedges directing callosal axons across the cerebral midline.^[43] Localized cue presentation involves glia concentrating attractive or repulsive molecules at precise sites, enhancing signal fidelity for growth cone steering. Ensheathment by glia promotes fasciculation, bundling axons via adhesive interactions that stabilize tracts and facilitate collective navigation.^[44] Representative examples illustrate these roles across species. In C. elegans, cephalic sheath (CEPsh) glia secrete UNC-6/netrin to guide RIA interneuron axons across the ventral nerve ring midline, ensuring proper circuit assembly.^[45] In the chick optic tectum, guidepost-like cells, including early-differentiating neurons and glia, provide intermediate targets that direct retinal ganglion cell axons to form topographic maps, with ablation studies revealing disrupted branching and targeting.^[46]

Experimental Model Systems

Commissure Development Models

Commissural axons in the developing chick spinal cord serve as a foundational model for understanding midline crossing during axon guidance. These axons, originating from dorsal neurons, extend ventrally toward the floor plate, a specialized midline structure that secretes the attractive cue netrin-1 to draw them across the central nervous system midline.^[47] Once axons cross, they turn longitudinally along the ventral spinal cord, avoiding reentry into the gray matter or recrossing the midline, a process mediated by repulsive signals such as Slit proteins expressed in the floor plate and ventral midline.^[48] This model highlights the spatial organization of guidance cues, with netrin-1 providing long-range attraction to the floor plate and Slit ensuring unidirectional crossing.^[47]^[48] Invertebrate systems, particularly the Drosophila ventral nerve cord, offer complementary insights into commissure formation, exemplified by the anterior commissure. In this pathway, commissural axons cross the midline under the control of the commissureless (comm) gene, which downregulates the Slit receptor Roundabout (Robo) to permit crossing despite midline Slit repulsion.^[49] After crossing, Robo levels increase, restoring sensitivity to Slit and preventing recrossing, thus establishing the commissure's integrity.^[50] This genetic regulation parallels vertebrate mechanisms and underscores the evolutionary conservation of repulsive signaling in midline navigation.^[49]^[50] Experimental techniques have been pivotal in dissecting these dynamics. In vitro stripe assays, where alternating lanes of substrate-bound guidance molecules are presented to growing axons, reveal preferential growth of chick commissural axons on netrin-1 stripes over controls, confirming its attractive role.^[47] Similarly, these assays demonstrate Slit-induced avoidance by post-crossing axons.^[48] Genetic knockouts in mice further validate these findings; netrin-1-deficient animals show commissural axons that stall before the floor plate and fail to cross, resulting in disrupted pathways.^[51] Slit-2 knockouts exhibit axons that recross the midline, emphasizing repulsion's necessity.^[52] Key insights from these models emphasize bidirectional control of axon behavior at the midline, where initial attraction facilitates crossing and subsequent repulsion directs longitudinal extension without recrossing.^[48] Temporal regulation of receptor expression is central, as seen in Drosophila where Comm transiently suppresses Robo to allow passage, a strategy mirrored in vertebrates through modulated receptor trafficking and sensitivity.^[49]^[50] These mechanisms ensure precise commissure formation, preventing errors that could lead to aberrant connectivity.^[48] Recent advances include human stem cell-derived models, such as midline assembloids formed by assembling human floor plate organoids (hFpOs) with spinal cord organoids (hSpOs). As of 2025, these hMAs recapitulate ventral patterning, commissural axon guidance across the midline, and bilateral connectivity, enabling the study of human-specific regulators. Profiling the hFpO secretome identified 27 human-enriched genes, and CRISPR screens revealed that loss of GALNT2 or PLD3 impairs floor plate-mediated axon guidance. This platform bridges gaps in understanding human neurodevelopment and potential disease mechanisms.^[53]

Topographic Mapping Systems

Topographic mapping systems in axon guidance rely on molecular gradients that direct axons to form ordered projections, ensuring precise connectivity between brain regions. These systems establish point-to-point relationships where neighboring neurons in one area connect to neighboring neurons in the target, often through repulsive or attractive cues that create topographic order. The retinotectal projection serves as the paradigmatic model, where retinal ganglion cell (RGC) axons from the retina map onto the superior colliculus (in mammals) or optic tectum (in non-mammals) in a retinotopic fashion.^[25] In the retinotectal system, topographic mapping along the anterior-posterior axis is achieved via countergradients of EphA receptors on RGC axons and ephrin-A ligands in the target. EphA receptors are expressed in a high-to-low gradient from temporal to nasal retina, with temporal axons expressing higher levels, while ephrin-A2 and ephrin-A5 ligands form a low-to-high gradient from anterior to posterior tectum/superior colliculus. This configuration generates repulsion that is stronger for temporal axons in posterior regions, restricting them to anterior terminations, whereas nasal axons, with lower EphA expression, can tolerate higher ephrin-A levels and project more posteriorly. Ephrin-A ligands act primarily as repellents, inducing growth cone collapse and branching inhibition in a concentration-dependent manner, with temporal axons showing greater sensitivity to low ephrin-A concentrations compared to nasal axons. Reverse signaling through ephrin-A also contributes, potentially promoting branching in appropriate zones.^[54]^[55]^[56] Experimental evidence from retinal explant cultures demonstrates gradient-dependent axon turning and arborization. In stripe assays, temporal RGC axons avoid posterior tectal membranes rich in ephrin-As, while nasal axons grow freely, confirming position-specific repulsion. More advanced setups with microfluidic gradients show that axons exhibit a graded transition from promotion to inhibition based on ephrin-A concentration and retinal origin, underscoring the role of relative signaling levels. In vivo, mutant mice lacking ephrin-A2 and ephrin-A5 display disrupted mapping, with temporal axons terminating ectopically in posterior regions and overall maps showing compressed or scattered projections rather than precise topography.^[54]^[55]^[57] Similar gradient-based mechanisms operate in other systems. In the somatosensory cortex, thalamocortical axons form topographic maps to establish barrel fields, where ephrin-A ligands in the cortex and EphA receptors in thalamic neurons create opposing gradients that guide axons from the ventral posteromedial nucleus to specific cortical barrels, ensuring whisker-specific innervation. Disruptions in ephrin-A signaling lead to barrel patterning defects and misaligned projections. In the olfactory bulb, topographic mapping of sensory neuron axons to glomeruli involves gradients of guidance cues like semaphorin-3F and its receptor neuropilin-2 along the dorsal-ventral axis, directing axons to form discrete zones based on odorant receptor expression and afferent activity. These systems illustrate how conserved gradient mechanisms adapt to diverse neural circuits.^[58]^[59]^[60]

Molecular and Genetic Foundations

Key Genes and Pathways

Axon guidance relies on a suite of conserved genes encoding receptors that detect extracellular cues and transduce signals to direct axonal navigation. Central to attractive guidance is the netrin receptor DCC (deleted in colorectal cancer), the mammalian ortholog of UNC-40 in Caenorhabditis elegans, which binds netrin-1 (the homolog of UNC-6) to promote axon attraction toward the midline via activation of downstream cytoskeletal regulators.^[61] In parallel, the Robo family of receptors, including Robo1-3, mediates repulsive responses to Slit ligands, preventing inappropriate midline crossing; this system was first elucidated in Drosophila, where Slit-Robo interactions enforce commissural axon repulsion.^[62] Repulsive semaphorin signaling operates through plexin receptors (e.g., Plexin-A) complexed with neuropilins (e.g., Nrp1), as demonstrated by the identification of Plexin-A as a semaphorin-3A receptor that collapses growth cones and redirects axons away from inhibitory environments.^[63] Bidirectional Eph/ephrin interactions further refine topographic mapping, with Eph receptors (e.g., EphA4) and ephrin ligands establishing gradients that sort axons in a repulsive manner during retinotectal projections.^[64] Downstream signaling pathways integrate these receptor activations to modulate cytoskeletal dynamics. Receptor tyrosine kinases such as TrkA, TrkB, and TrkC, activated by neurotrophins like NGF and BDNF, enhance axon outgrowth and guidance by phosphorylating intracellular domains that recruit adapters like Shc, thereby activating PI3K and MAPK cascades to promote filopodial extension.^[65] GTPase cascades, particularly involving Rac and Cdc42, serve as key effectors of protrusion and turning; for instance, netrin-1/DCC signaling activates Rac/Cdc42 to polymerize actin and advance the growth cone, while repulsive cues like semaphorins inhibit these GTPases via RhoA to induce collapse.^[31] These small GTPases link guidance receptors to the actin cytoskeleton, enabling precise responses to local gradients.^[66] The molecular machinery is highly conserved across species, underscoring its fundamental role in neural wiring. In C. elegans, UNC-6/netrin and UNC-40/DCC direct ventral and dorsal axon migrations, a paradigm mirrored in vertebrates where netrin-1/DCC guides commissural axons across the floor plate during spinal cord development.^[67] Similar orthology extends to Robo/Slit and Eph/ephrin systems, with Drosophila mutants revealing principles later confirmed in mammalian models, such as Slit2 repelling Robo-expressing axons from the corpus callosum.^[62] Transcriptional regulation fine-tunes receptor expression in neuronal subtypes to ensure context-specific guidance. LIM-homeodomain factors Isl1 and Isl2 coordinately control the expression of guidance receptors and ligands; for example, Isl1/2 directly regulate Slit2 and Robo3 in branchiomotor neurons, restricting their domains to prevent aberrant projections in the hindbrain.^[68] In spinal motor neurons, Isl1/2 orchestrate axon targeting by activating subsets of Eph receptors, as shown in Drosophila where Isl mutants disrupt motor axon pathfinding.^[69] This selective control by Isl1/2 highlights how transcription factors pattern guidance competence across neuronal populations.

Genetic Mutations and Associations

Mutations in genes encoding axon guidance molecules have been instrumental in elucidating their roles in neural development, often leading to disrupted midline crossing and abnormal projections. For instance, knockout of the Robo3 gene in mice results in a failure of commissural axons to cross the midline, causing them to project ipsilaterally instead of contralaterally, as demonstrated in early studies of Rig-1/Robo3-deficient embryos where dorsal commissural axons extend longitudinally on the same side of the spinal cord. This phenotype highlights Robo3's specific function in regulating the post-crossing repulsion necessary for proper decussation. Similarly, mutations in the DCC gene, a Netrin-1 receptor, have been identified in humans with isolated agenesis of the corpus callosum (ACC), leading to incomplete penetrance and phenotypes such as mirror movements due to impaired callosal axon guidance across the midline.^[70] In human neurodevelopmental disorders, variants in ROBO1, another Slit receptor, are associated with altered brain connectivity, particularly in autism spectrum disorders (ASD). Genetic analyses have shown that ROBO1 SNPs exhibit significant association with ASD, accompanied by reduced ROBO1 mRNA expression in autistic postmortem brain tissue, suggesting a role in dysregulated axonal pathfinding and synaptic connectivity. These findings extend to compound heterozygous variants in ROBO1 causing broader neurodevelopmental syndromes with absence of transverse pontine fibers, underscoring the gene's impact on brainstem axon guidance.^[71]^[72]^[73] Forward genetic screens in model organisms have been pivotal in identifying mutations affecting axon guidance. In Drosophila, large-scale mutagenesis screens recovered the commissureless (comm) mutants, where loss of Comm function prevents commissural axons from crossing the midline, resulting in a complete absence of most CNS commissures while longitudinal tracts remain intact.^[74] These screens, covering a substantial portion of the genome, revealed Comm's role in regulating Robo receptor trafficking to enable midline crossing. More recently, genome-wide association studies (GWAS) have linked variants in axon guidance genes to neurodevelopmental traits; for example, ROBO1 polymorphisms are associated with dyslexia and related connectivity issues, implicating guidance pathways in cognitive phenotypes.^[75] Many axon guidance defects follow Mendelian inheritance patterns, providing clear genetic models for study. Kallmann syndrome, an X-linked disorder caused by mutations in the KAL1 gene encoding anosmin-1, exemplifies this, as loss of anosmin-1 disrupts the tangential migration of gonadotropin-releasing hormone (GnRH) neurons along olfactory axons, leading to hypogonadotropic hypogonadism and anosmia due to failed axon guidance from the nasal placode to the hypothalamus. This mutation affects extracellular matrix interactions critical for neuronal migration akin to axon pathfinding, with affected individuals showing inheritance consistent with X-linked recessive patterns.^[76]^[77]^[78]

Pathophysiological Implications

Disorders Linked to Guidance Defects

Defects in axon guidance can lead to a range of neurological and developmental disorders characterized by abnormal neural connectivity, particularly failures in midline crossing and topographic organization of axonal tracts.^[79] One prominent example is agenesis of the corpus callosum (ACC), a condition resulting from midline crossing failure during callosal axon development, where pioneer axons fail to navigate the midline due to disrupted guidance cues, leading to partial or complete absence of the corpus callosum.^[80] This malformation often manifests with cognitive impairments, seizures, and motor delays, and is diagnosed via MRI revealing absent or hypoplastic callosal fibers and associated Probst bundles—longitudinal axonal tracts that fail to cross.^[81] Another major disorder is horizontal gaze palsy with progressive scoliosis (HGPPS), caused by mutations affecting axonal pathfinding in the brainstem and spinal cord, resulting in uncrossed corticospinal and somatosensory tracts that impair horizontal eye movements and lead to spinal curvature.^[82] Symptoms typically emerge in infancy with inability to move eyes laterally, progressing to scoliosis by adolescence, and diagnosis involves clinical examination, genetic testing, and MRI showing aberrant tract decussation.^[83] Congenital mirror movements represent another condition linked to axon guidance defects, where involuntary mirroring of voluntary movements occurs due to impaired midline crossing of corticospinal axons, often stemming from disruptions in netrin-1 signaling pathways essential for commissural axon attraction to the midline.^[84] Affected individuals exhibit synchronous hand movements during intentional actions, persisting into adulthood, with diagnosis confirmed by neurological assessment and electromyography demonstrating bilateral muscle activation.^[85] Associations have also been observed between axon guidance disruptions and schizophrenia, particularly involving altered topographic maps in cortical connectivity, where aberrant axonal bundling and pruning lead to disorganized functional networks underlying psychotic symptoms.^[86] Polygenic variations in guidance-related genes contribute to these connectivity deficits, exacerbating risk through cumulative effects on prefrontal and sensory pathways.^[87] In DiGeorge syndrome, cleft palate arises from errors in neural crest cell migration, which shares mechanistic overlap with axon guidance processes, as migrating neural crest cells rely on similar repulsive and attractive cues to populate pharyngeal arches and form palatal structures.^[88] This results in orofacial anomalies visible at birth, with diagnosis incorporating genetic microarray for 22q11.2 deletion and imaging to assess palatal gaps alongside associated cardiac and immune defects.^[89] Monogenic disorders linked to axon guidance defects, such as Kallmann syndrome—involving failed migration of gonadotropin-releasing hormone neurons along olfactory axonal paths—are rare, with an estimated prevalence of 1 in 8,000 to 30,000 males and even lower in females.^[90] In contrast, polygenic contributions from axon guidance pathway variants play a more common role in disorders like autism spectrum disorder, where cumulative genetic effects disrupt synaptic connectivity and topographic organization, influencing up to 10-20% of heritability in population studies.^[91]

Emerging Therapeutic Strategies

Emerging therapeutic strategies for axon guidance defects aim to promote neural repair by modulating inhibitory environments, enhancing intrinsic growth programs, and repurposing developmental cues for regeneration in conditions such as spinal cord injury and optic nerve damage.^[92] These approaches leverage insights from molecular pathways to overcome barriers like glial scars and repulsive signals, with a focus on translating preclinical successes into clinical applications.^[93] Regeneration strategies include enzymatic degradation of inhibitory extracellular matrix components. Chondroitinase ABC (ChABC), a bacterial enzyme, degrades chondroitin sulfate proteoglycans (CSPGs) in glial scars, which otherwise inhibit axonal regrowth following spinal cord injury; preclinical studies demonstrate improved locomotor recovery and axonal plasticity in rodent models treated with ChABC.^[93] Similarly, infusion of netrin-1, a guidance cue, promotes axonal regrowth across lesion sites in chronic spinal cord injury models, restoring partial hindlimb function in rats by enhancing neurite extension and synaptic formation via DCC receptor signaling.^[94] Gene therapy offers targeted interventions to correct or augment guidance signaling. Adeno-associated virus (AAV)-mediated delivery of axon guidance molecules, such as L1CAM, enhances neurite extensions and integration of transplanted neurons in brain injury models, promoting functional axonal connectivity.^[95] While direct CRISPR editing of ROBO receptors remains preclinical, related genetic manipulations of guidance pathways, like silencing Plexin-B1 (a semaphorin receptor), have shown promise in promoting retinal ganglion cell axon regrowth toward the optic chiasm in optic nerve injury models.^[92] Pharmacological targeting of intracellular effectors downstream of guidance cues addresses regeneration failure. Small molecules inhibiting RhoA/ROCK signaling override repulsive cues from myelin and scars, stimulating neurite outgrowth in adult dorsal root ganglion neurons and improving functional recovery in spinal cord injury; for instance, RhoA knockdown reverses CSPG-mediated growth inhibition.^[96] These inhibitors promote actin cytoskeleton remodeling essential for axon advance.^[97] Clinical translation of semaphorin inhibitors for optic nerve repair is advancing from preclinical stages, with neutralizing antibodies against semaphorin-3A protecting retinal ganglion cells and enhancing axonal survival post-axotomy in rodent models, though human trials are limited to phase I safety assessments for related neuroprotective agents.^[98] Challenges include off-target effects on non-neuronal tissues, delivery across the blood-brain barrier, and ensuring precise reinnervation without aberrant sprouting.^[92] Combinatorial therapies integrating these strategies hold potential for synergistic outcomes in neurodegenerative repair.^[93]

References

[1]
New insights into the molecular mechanisms of axon guidance ...
In this review, we highlight recent advances that shed light on the rich complexity of mechanisms that regulate axon guidance receptor distribution, activation ...
[2]
Structural Perspectives on Axon Guidance - Annual Reviews
Oct 6, 2016 · Axon guidance relies on a combinatorial code of receptor and ligand interactions that direct adhesive/attractive and repulsive cellular ...<|control11|><|separator|>
[3]
Axon guidance mechanisms and molecules: lessons from ... - Nature
Nov 1, 2003 · Studies in invertebrates have shown the important role of pre-existing axons in guiding subsequent axon outgrowth. Once pioneer neurons have ...
[4]
Axon Guidance - an overview | ScienceDirect Topics
Axon guidance is defined as the process by which axonal growth cones detect and interpret extracellular signals, facilitating the precise wiring of the nervous ...Missing: core paper
[5]
Axonal growth and guidance - Scholarpedia
Sep 14, 2014 · Definition. Axon guidance is the process by which growing nerve fibers find their targets in the developing brain.
[6]
Role of L1 in Axon Pathfinding and Fasciculation - Oxford Academic
The common steps in axon guidance include initial outgrowth and formation of a growth cone, axon bundling or fasciculation, defasciculation, branching, target ...
[7]
The discovery of the growth cone and its influence on ... - PMC - NIH
May 15, 2015 · A particular type of interaction between the growth cone and its target cells was envisioned by Sperry in 1963 as part of the “chemoaffinity ...
[8]
CHEMOAFFINITY IN THE ORDERLY GROWTH OF NERVE FIBER ...
CHEMOAFFINITY IN THE ORDERLY GROWTH OF NERVE FIBER PATTERNS AND CONNECTIONS · Full text · Images in this article · Selected References · ACTIONS · PERMALINK.Missing: hypothesis | Show results with:hypothesis
[9]
Mechanisms of Axon Growth and Regeneration - PubMed Central
Nov 9, 2022 · Recent progress has enabled a better understanding of how developmental mechanisms direct axon regeneration. Focusing on neuronal polarization, ...Missing: timeline | Show results with:timeline
[10]
https://pmc.ncbi.nlm.nih.gov/articles/PMC9665933/
[11]
https://doi.org/10.1242/dev.151415
[12]
https://doi.org/10.1002/dneu.22473
[13]
Axons: the cable transmission of neurons - Queensland Brain Institute
Depending on the type of neuron, axons greatly vary in length - many are just a millimetre or so, but the longest ones, such as those that go from the brain ...
[14]
Adhesion-clutch between DCC and netrin-1 mediates ... - Frontiers
We demonstrate that substrate-bound netrin-1 induces axonal haptotaxis by facilitating physical interactions between the netrin-1 receptor, DCC, and the ...Missing: PI3K seminal
[15]
Netrin-1 Attracts Axons through FAK-Dependent Mechanotransduction
Aug 22, 2012 · The mechanical tethering of axon guidance cues raises the possibility that growth cones pull directly on the cues that guide them. Indeed, in ...Results · Traction On Netrin-1... · Discussion<|control11|><|separator|>
[16]
https://doi.org/10.1016/s0092-8674(00)81795-x
[17]
The Growth Cone Cytoskeleton in Axon Outgrowth and Guidance
Axon outgrowth results from progress through three stages: protrusion, engorgement, and consolidation. Protrusion is the extension of new membrane at the edges ...
[18]
The trip of the tip: understanding the growth cone machinery - PMC
The central player in the road trip of axon guidance is the growth cone, a dynamic structure located at the tip of the growing axon.
[19]
Guiding Neuronal Growth Cones by Ca2+ Signals - PubMed Central
One possible mechanism by which filopodial Ca2+ transients can regulate growth cone motility and guidance is through local activation of calpain [48]. Calpain ...Missing: apparatus | Show results with:apparatus
[20]
Growth Cone Form Is Behavior-Specific and, Consequently, Position ...
Feb 1, 1997 · Several basic behaviors—extension, pausing, retraction, and branching—were observed along the retinal axon pathway (Fig.2). Extension was ...Missing: stalling | Show results with:stalling
[21]
Rho and Ras GTPases in Axon Growth, Guidance, and Branching
This article focuses on the role of Rho and Ras family members in four different aspects of axonogenesis: initiation, elongation, guidance, and branching.Missing: cAMP threshold
[22]
Synergistic integration of Netrin and ephrin axon guidance signals ...
Dec 3, 2015 · The increased LMC axon repulsion from ephrins in the presence of Netrin-1 could be due to Netrin and Eph signaling acting simultaneously at the ...
[23]
Cellular Strategies of Axonal Pathfinding - PMC - PubMed Central
PIONEER NEURONS. In any developing axon tract, the first extending axons will necessarily pioneer the route that all axons in the tract will ultimately traverse ...
[24]
Pathfinding in the central nervous system and periphery by identified ...
We have studied the pattern of axon outgrowth from the identified embryonic Drosophila motorneurons, RP1, RP3, RP4 and RP5, from the onset of axonogenesis ...
[25]
The Multiple Roles of Cell Adhesion Molecules in Neural Circuit ...
Axon Fasciculation for Tract Formation. Pioneer axons play a critical role in neural circuit assembly, not only by defining first itineraries toward ...
[26]
The Role of L1 in Axon Pathfinding and Fasciculation - PubMed - NIH
The neural cell adhesion molecule L1 has been found to play important roles in axon growth and fasciculation. Our main objective was to determine the role ...Missing: seminal paper CAM
[27]
Organization of pioneer retinal axons within the optic tract of ... - PNAS
Retinal ganglion cell axons must make a decision at the embryonic optic chiasm to grow into the appropriate optic tract. To gain insight into the cues that ...
[28]
Netrin-1 is required for commissural axon guidance in the ... - PubMed
Netrin-1, a diffusible protein made by floor plate cells, can attract spinal commissural axons and repel trochlear axons in vitro, but its role in vivo is ...Missing: 1994 | Show results with:1994
[29]
Floor plate-derived netrin-1 is dispensable for commissural axon ...
Oct 26, 2017 · We found that hindbrain and spinal cord commissural axons develop normally in absence of floor plate-derived netrin-1.
[30]
Guidance of Callosal Axons by Radial Glia in the Developing ...
For example, evidence that glia provide a permissive substrate for axonal outgrowth in vivo has come from studies in the mouse corpus callosum. Disruption of ...<|separator|>
[31]
Transient cell–cell interactions in neural circuit formation - PMC
Each guidepost cell attracts the growth cone, allowing stepwise guidance of the axon. Examples of intermediate targets in axon guidance include the floor plate ...Missing: Delta- Notch
[32]
Glial cell adhesive molecule unzipped mediates axon guidance in ...
Nov 29, 2010 · Guidepost cells are required for axon guidance in the nervous systems. In ... The glycosyltransferase Fringe promotes Delta-Notch signaling ...
[33]
Cortical Axon Guidance by the Glial Wedge during the Development ...
Apr 15, 2001 · We have identified two early differentiating populations of midline glial cells that may act as intermediate guideposts for callosal axons.
[34]
The role of glial cells in axon guidance, fasciculation and targeting
We review here the experimental evidence investigating roles of glia in axon guidance. Some molecules are known to influence either the neurons or the glia, but ...
[35]
The glia of Caenorhabditis elegans - PMC - NIH
At least part of the axon guidance defect is due to the lack of UNC-6/Netrin secretion from the ventral CEPsh glia, which normally guides axons within the nerve ...Missing: ALM | Show results with:ALM
[36]
Axon Guidance: Guidance Cues and Guidepost Cells - ResearchGate
Sep 12, 2025 · Pioneering studies in insects suggested that guidepost cells, not final targets, are crucial to guide axons to their destination, a notion that ...
[37]
Eph and ephrin signaling in the formation of topographic maps - PMC
EphAs and ephrin-As are graded molecular cues required for topographic mapping while the dorsal-ventral axis is mapped in part via EphB and ephrin-Bs.Missing: seminal papers
[38]
Complementary gradients in expression and binding of ELF-1 and ...
Complementary gradients in expression and binding of ELF-1 and Mek4 in development of the topographic retinotectal projection map. Cell. 1995 ...
[39]
Balancing of ephrin/Eph forward and reverse signaling as the ...
Jan 15, 2012 · The retinotectal projection, which topographically maps retinal axons onto the tectum of the midbrain, is an ideal model system with which ...
[40]
Role of EphA/ephrin-A signaling in the development of topographic ...
Topographic mapping of somatosensory CT projections is regulated by the level of EphA7 in cortical neurons. By using in utero electroporation-mediated gene ...Missing: seminal | Show results with:seminal
[41]
Area Specificity and Topography of Thalamocortical Projections Are ...
We provide in vivo evidence that Eph receptors in the thalamus and ephrins in the cortex control intra-areal topographic mapping of thalamocortical (TC) axons.
[42]
Topographic Mapping—The Olfactory System - PMC - NIH
Axonal projection along the dorsal–ventral axis is determined by axon guidance molecules such as Nrp2, Sema3F, and Robo2 expressed in a graded manner in the OE.Missing: seminal | Show results with:seminal
[43]
Signaling mechanism of the netrin-1 receptor DCC in axon guidance
DCC is involved in the dual functionality of netrin-1 as a chemo-attractant as well as a repellent in axon guidance, referred to as bi-functionality.
[44]
Slit Proteins Bind Robo Receptors and Have an Evolutionarily ...
These results support the hypothesis that Slit proteins have an evolutionarily conserved role in axon guidance as repulsive ligands for Robo receptors.
[45]
Plexin A Is a Neuronal Semaphorin Receptor that Controls Axon ...
Plexin A is a neuronal receptor for class I Semaphorins, controlling motor and CNS axon guidance. It is a large type 1 membrane glycoprotein.
[46]
Eph/ephrin signalling during development
Nov 15, 2012 · Trans interactions of Eph receptors with ephrins at cell-cell interfaces promote a variety of cellular responses, including repulsion, ...
[47]
The role of neurotrophins in axonal growth, guidance, and ... - PubMed
Neurotrophins, as other neurotrophic factors, have been shown that they reduce neuronal injury by exposure to excitotoxins, glucose deprivation, or ischemia.
[48]
Axon guidance: GTPases help axons reach their targets - Cell Press
One class of molecules whose study promises to yield substantial insights into axon guidance is the family of Ras-related GTPases, including Rho, Rac and Cdc42.
[49]
The Genetics of Axon Guidance and Axon Regeneration in ...
Genetic analysis in Caenorhabditis elegans has played a key role in elucidating conserved pathways regulating axon guidance.
[50]
Slit and semaphorin signaling governed by Islet transcription factors ...
Several lines of evidence show that Isl1 and Isl2 control the transcription of similar sets of genes. Their DNA binding-homeodomains (HD) in multiple species ...
[51]
Islet Coordinately Regulates Motor Axon Guidance and Dendrite ...
Feb 14, 2017 · We show that the LIM homeodomain factor Islet (Isl) controls targeting of both axons and dendrites in Drosophila motor neurons through ...
[52]
commissureless controls growth cone guidance across the CNS ...
... mutations that disrupt CNS axon pathways in Drosophila. The comm gene has a unique mutant phenotype: the complete absence of most axon commissures, while ...Missing: discovery paper
[53]
Mutations in DCC cause isolated agenesis of the corpus callosum ...
We identified DCC mutations in four families and five sporadic individuals with isolated agenesis of the corpus callosum (ACC) without intellectual disability.
[54]
Genetic analyses of roundabout (ROBO) axon guidance receptors in ...
Oct 5, 2008 · ROBO3 and ROBO4 SNPs showed associations with autism. ROBO1 and ROBO2 expressions were reduced in autistic patients, suggesting a possible role ...Missing: seminal paper
[55]
Genetic Analyses of Roundabout (ROBO) Axon Guidance Receptors ...
Aug 6, 2025 · Expressions of ROBO1 (P = 0.018) and ROBO2 (P = 0.023) were significantly reduced in the autistic group; the possibility of using the altered ...
[56]
Compound Heterozygous Variants in ROBO1 Cause a ...
These findings strongly suggest that human ROBO1 variants can result in a novel neurodevelopmental disorder due to impaired axonal guidance. Section ...
[57]
Expression of Genes Involved in Axon Guidance - PubMed Central
May 18, 2020 · Many genome wide association studies have identified the association of axon guidance-related genes to different neurological disorders, i.e., ...
[58]
Kallmann's syndrome, a neuronal migration defect - PMC - NIH
In this review we focus our attention on insights evoked by recent studies, which propose a new direct role for anosmin-1 in the migration GnRH neurons, and a ...
[59]
product of X-linked Kallmann's syndrome gene (KAL1) affects the ...
These results strongly suggest an involvement of anosmin-1 in the control of the migratory behaviour of GnRH neurons and provide novel information on the ...
[60]
Nasal Placode Development, GnRH Neuronal Migration ... - Frontiers
Disruption of GnRH results in hypogonadism and if accompanied by anosmia is termed Kallmann Syndrome (KS). From their origin in the nasal placode, GnRH neurons ...
[61]
Human Genetic Disorders of Axon Guidance - PMC - PubMed Central
Both complete and partial agenesis (CCA, pCCA) can likely occur secondary to disruption in any one of the multiple steps in callosal development, including ...
[62]
Corpus Callosum Agenesis: An Insight into the Etiology and ...
The most frequent causes of corpus callosum agenesis (ACC) are gene mutations that are related to pathways of axon guidance, ciliary development, cell adhesion ...
[63]
Agenesis and Dysgenesis of the Corpus Callosum: Clinical, Genetic ...
Among the most frequent clinical findings in patients with ACC are mental retardation (60%), visual problems (33%), speech delay (29%), seizures (25%), and ...<|separator|>
[64]
ROBO3 Mutation Causing Horizontal Gaze Palsy and Brainstem ...
Dec 23, 2022 · Horizontal gaze palsy with progressive scoliosis (HGPPS1) results from axonal guidance signalling defects caused by ROBO3 mutations.
[65]
Horizontal gaze palsy with progressive scoliosis - MedlinePlus
Apr 7, 2025 · Horizontal gaze palsy with progressive scoliosis (HGPPS) is a disorder that affects the eyes and the spine.
[66]
Mutations in the netrin-1 gene cause congenital mirror movements
Netrin-1 is a secreted protein that was first identified 20 years ago as an axon guidance molecule that regulates midline crossing in the CNS.
[67]
Congenital mirror movement disorder: MedlinePlus Genetics
May 19, 2025 · Congenital mirror movement disorder is a condition in which intentional movements of one side of the body are mirrored by involuntary movements of the other ...
[68]
Disrupted Axonal Fiber Connectivity in Schizophrenia - PMC - NIH
Schizophrenia is believed to result from abnormal functional integration of neural processes thought to arise from aberrant brain connectivity.
[69]
Disorders of Axon Guidance - The Genetics of Neurodevelopmental ...
Jul 24, 2015 · Genetic, functional, and experimental evidence has linked axon guidance genes to a wide array of neurodevelopmental disorders with ...
[70]
22q11.2 deletion syndrome - PMC - PubMed Central - NIH
b | Cardiac neural crest cells (NCCs) delaminate from the hindbrain and migrate ventrolaterally to populate the pharyngeal arches. The T-box transcription ...
[71]
Craniofacial Phenotypes and Genetics of DiGeorge Syndrome - PMC
Tbx1 is required for proper neural crest migration and to stabilize spatial patterns during middle and inner ear development. Mech. Dev. 2005;122:199–212 ...Missing: axon | Show results with:axon
[72]
A rare disease of Kallmann syndrome: A case report - ScienceDirect
KS has an incidence of 1 in 8000 to 30,000 in males and 1 in 40,000 to 120,000 in females. The estimated incidence of KS in males is 4-5 times higher than in ...
[73]
Polygenic scores for autism are associated with reduced neurite ...
Feb 24, 2025 · This study provides evidence for shared common variant genetics between autism and cortical neurite density.
[74]
Review Emerging therapeutic strategies for optic nerve regeneration
Sep 26, 2024 · Here, we summarize recent advances in interventions targeting cell-intrinsic and cell-extrinsic mechanisms to enhance RGC axon regeneration.
[75]
Chondroitinase ABC in spinal cord injury - PMC - NIH
Jun 9, 2025 · Chondroitinase ABC (ChABC), a bacterial enzyme capable of degrading CSPGs, has emerged as a promising therapeutic strategy for enhancing neural plasticity and ...
[76]
Netrin-1 Therapy Restores Partial Hindlimb Movement in a Rat ...
Nov 5, 2025 · Therefore, in this study, we investigated the therapeutic potential of Netrin-1 as a treatment to enhance axonal regrowth across the lesion site ...
[77]
Viral delivery of L1CAM promotes axonal extensions by embryonic ...
Mar 23, 2023 · Takahashi and colleagues show that the AAV-mediated delivery of L1CAM into mouse brain enhances neurite extensions by the grafted cortical neurons.Missing: pioneer | Show results with:pioneer<|control11|><|separator|>
[78]
RhoA as a target to promote neuronal survival and axon regeneration
RhoA mediates both apoptosis and failure of axon regeneration after SCI. Activation of PTEN by RhoA/ROCK has been reported in cell lines but not yet in neurons ...
[79]
Axon growth inhibition by RhoA/ROCK in the central nervous system
Blocking RhoA/ROCK signaling can reverse the inhibitory effects of these molecules on axon outgrowth, and promotes axonal sprouting and functional recovery in ...
[80]
Anti-semaphorin 3A Antibodies Rescue Retinal Ganglion Cells from ...
In this study, we examined the involvement of semaphorins in an in vivo experimental animal model of complete axotomy of the rat optic nerve. We demonstrate ...