Fact-checked by Grok 2 weeks ago

CD27

CD27 is a type I transmembrane glycoprotein and member of the tumor necrosis factor receptor (TNFR) superfamily, functioning as a costimulatory immune-checkpoint receptor primarily expressed on the surface of T cells, B cells, and natural killer (NK) cells.^[1] It binds to its ligand CD70 (also known as CD27L), a member of the TNF superfamily, to deliver signals that promote lymphocyte activation, proliferation, survival, and differentiation.^[2] Encoded by the CD27 gene located on chromosome 12p13.2, the protein forms disulfide-linked homodimers and features three cysteine-rich extracellular domains characteristic of TNFR family members.^[3] Upon ligation with CD70, CD27 transduces intracellular signals through adaptor proteins such as TRAF2 and TRAF5, activating pathways including NF-κB and JNK/MAPK, which are essential for T cell effector function, memory formation, and long-term immunity.^[1] In T cells, CD27 costimulation enhances antigen-specific responses, particularly in CD8+ T cells during viral infections, by preventing apoptosis and supporting clonal expansion.^[4] For B cells, CD27 signaling promotes germinal center reactions, plasma cell differentiation, and immunoglobulin production, while also marking memory B cells, though its expression can be modulated by cytokines like IL-21.^[1]^[5] The CD27-CD70 interaction is tightly regulated, with CD70 expression limited to activated antigen-presenting cells and lymphocytes, ensuring transient costimulation to avoid chronic immune activation.^[4] Dysregulation of this axis contributes to immune disorders; for instance, germline mutations in CD27, such as nonsense variants (e.g., W8X), cause lymphoproliferative syndrome 2 (LPFS2), characterized by Epstein-Barr virus-driven lymphoproliferation, hemophagocytic lymphohistiocytosis, and increased lymphoma risk.^[3] Conversely, in oncology, CD27 agonists are being explored to boost antitumor T cell responses, often in combination with checkpoint inhibitors, highlighting its dual role in immune homeostasis and therapeutic potential.^[6]

Molecular Biology

Gene and Protein Overview

The CD27 gene, officially designated TNFRSF7, is located on the short arm of human chromosome 12 at position 12p13.31. It spans approximately 7.8 kb of genomic DNA, with its canonical transcript comprising 6 exons.^[1] CD27 was first identified and cloned in 1991 from a human T cell cDNA library using expression cloning and immunoselection techniques, revealing it as a T cell activation antigen and establishing its membership in the tumor necrosis factor receptor (TNFR) superfamily.^[7] The protein product of the CD27 gene is a type I transmembrane glycoprotein with an apparent molecular weight of 55 kDa, consisting of 260 amino acids in its predominant isoform; it forms disulfide-linked homodimers on the cell surface.^[2]^[8] CD27 demonstrates evolutionary conservation across mammalian species, with orthologs identified in over 500 species; notably, the extracellular domain shares high sequence similarity, exhibiting approximately 65% identity between human and murine forms.^[1]

Structure and Domains

CD27 is a type I transmembrane glycoprotein belonging to the tumor necrosis factor receptor (TNFR) superfamily, characterized by a modular architecture that includes an extracellular ligand-binding region, a transmembrane-spanning segment, and a short cytoplasmic domain. The extracellular region consists of three tandem cysteine-rich domains (CRDs), each approximately 40 amino acids long, which form an elongated, ladder-like structure stabilized by disulfide bonds. These CRDs—CRD1 (residues 1–43), CRD2 (residues 44–85), and CRD3 (residues 86–101)—exhibit the characteristic A-B-C-B-C-A disulfide bonding pattern typical of TNFR family members, with CRD1 and CRD2 primarily mediating ligand interactions through conserved residues in their beta-sheet frameworks.^[9] The transmembrane domain, spanning residues 192–212, anchors CD27 in the plasma membrane and facilitates dimerization via a conserved cysteine residue (Cys185) in the preceding stalk region, forming disulfide-linked homodimers essential for receptor stability. Beyond this, the intracellular tail comprises 48 amino acids (residues 213–260), a relatively short extension lacking a canonical death domain found in other TNFRs like TNFR1, but enriched with proline and serine residues that support recruitment of adaptor proteins for downstream signaling. This proline- and serine-rich composition enables interactions with TRAF family adaptors without inducing apoptosis via death domain pathways.^[10]^[11] Insights into the three-dimensional architecture of CD27 derive from crystallographic studies, including a 1.8 Å resolution structure of the extracellular domain determined in 2017, which reveals a monomeric unit poised for trimerization upon ligand engagement, mimicking the oligomeric assembly of TNFRs with their trimeric TNF-like ligands. This structure highlights a unique epitope on CRD2 (residues 57–68) that serves as a binding site for neutralizing antibodies, distinct from the core ligand interface and offering potential for therapeutic targeting. Additionally, post-translational N-glycosylation occurs at multiple asparagine-linked sites in the extracellular domain, such as Asn75 and Asn95, contributing to protein folding, stability, and modulation of receptor dimerization.^[9]^[8]

Ligand and Expression

CD70 Ligand and Binding

CD70, designated TNFSF7, serves as the sole known ligand for CD27, a member of the tumor necrosis factor receptor (TNFR) superfamily. This ligand is a type II transmembrane glycoprotein within the TNF ligand superfamily, characterized by its ability to form homotrimers essential for receptor engagement.^[12]^[13] The molecular interaction between CD27 and CD70 exhibits high affinity, with a dissociation constant (K_d) of approximately 133 nM for the glycosylated form of CD27, as determined by surface plasmon resonance (SPR) analysis. Binding primarily involves the cysteine-rich domains (CRDs) 2 and 3 of CD27's extracellular region interfacing with adjacent protomers of the CD70 trimer, with minimal contribution from CRD1 via a single van der Waals contact. This engagement occurs without significant influence from CD27's N-linked glycosylation, as deglycosylated CD27 displays a comparable K_d of 118 nM.^[12] Structurally, the CD27:CD70 complex adopts a 3:3 stoichiometry, wherein three CD27 monomers associate with one CD70 homotrimer, facilitating receptor oligomerization on the cell surface. The crystal structure of this complex reveals CD70 in a canonical TNF ligand fold, with a unique conserved disulfide bond (C133-C168) that stabilizes its architecture, a feature retained in murine CD70 to support cross-species interaction. This arrangement promotes clustering of CD27 receptors, inducing moderate conformational shifts in the inter-CRD hinges (e.g., a 10.7° difference in the CRD2-CRD3 angle), which propagate to the intracellular tail to enable downstream signaling initiation. No alternative ligands for CD27 have been identified, emphasizing the specificity of this pairwise interaction.^[12] The evolutionary co-conservation of the CD27-CD70 pair across mammals, including shared structural motifs like the stabilizing disulfide in CD70, highlights their specialized role in fine-tuning immune responses.^[12]

Cellular Expression Patterns

CD27 is constitutively expressed on various subsets of immune cells, including naïve CD4+ and CD8+ T cells, central and effector memory T cells, regulatory T cells, natural killer (NK) cells, and natural killer T (NKT) cells in both humans and mice.^[5]^[14]^[15]^[16] In contrast, expression is low or absent on resting B cells, monocytes, and granulocytes, with upregulation occurring specifically on activated B cells during the germinal center reaction, marking memory B cell formation.^[5]^[14]^[17] Beyond hematopoietic cells, CD27 expression has been detected in non-hematopoietic contexts, such as embryonic hematopoietic stem cells co-expressing c-Kit and Gata2 during early ontogeny in mice, as well as on certain tumor cells in solid and hematologic malignancies.^[18]^[6] These patterns highlight CD27's role in both steady-state immune surveillance and pathological conditions, though its presence in non-immune tissues remains limited and context-dependent. The regulation of CD27 surface expression is tightly controlled by immune signaling pathways. Induction occurs through T cell receptor (TCR) and B cell receptor (BCR) engagement, cytokine stimulation such as interleukin-2 (IL-2), and transcription factors including NF-κB, which promote its upregulation on lymphocytes during activation.^[14]^[19]^[20] Post-activation downregulation is mediated by metalloproteases ADAM10 and ADAM17, which cleave CD27 from the cell surface, reducing its expression on effector cells.^[21] Expression patterns are largely conserved between humans and mice, with CD27 present on similar immune cell subsets in both species; however, notable differences include higher and more persistent CD27 expression on primed human B cells compared to mice, and exclusive expression on hematopoietic progenitors in mice but not in humans.^[14]^[22]

Physiological Functions

Role in T Cell Immunity

CD27 serves as a critical co-stimulatory receptor on T cells, where its interaction with CD70 provides essential signals during T cell priming, enhancing proliferation, survival, and differentiation into effector cells. This co-stimulation is particularly vital in the absence of other pathways like CD28, as it rescues activated T cells from apoptosis at the onset of division and supports robust expansion of antigen-specific clones. In experimental models, CD27 engagement promotes the transition from naive to effector T cells, amplifying cytokine production and cytotoxic potential without inducing exhaustion.^[23]^[24]^[25] In CD8+ T cells, CD27 signaling is indispensable for memory formation and long-term cytotoxic function, playing a pivotal role in antiviral and antitumor immunity. It drives the generation of central memory CD8+ T cells by sustaining survival signals during the contraction phase post-infection, leading to enhanced recall responses upon re-exposure to pathogens like influenza or lymphocytic choriomeningitis virus. This pathway also bolsters antitumor activity by increasing persistence and effector differentiation of tumor-infiltrating CD8+ T cells, as demonstrated in adoptive transfer models where CD27 costimulation improved tumor clearance. Critically, CD27 promotes antiviral responses by facilitating primary and secondary expansion of virus-specific CD8+ T cells, ensuring effective control of acute infections.^[26]^[27]^[28]^[29] For CD4+ T cells, CD27 costimulation supports differentiation into Th1 subsets by enhancing interferon-γ production, while enabling metabolic reprogramming essential for their effector functions. Recent studies highlight how CD27 signaling upregulates glycolysis, the pentose phosphate pathway, and tricarboxylic acid (TCA) cycle flux via mTOR activation, thereby boosting de novo nucleotide and protein synthesis to meet the bioenergetic demands of proliferating CD4+ effectors. This metabolic shift, observed in human and murine models, ensures sustained fitness during inflammatory challenges.^[30]^[31] CD27 also modulates regulatory T cell (Treg) function, where its expression on Tregs maintains their suppressive capacity in peripheral tolerance but presents a target for enhancing antitumor immunity. Treg-intrinsic CD27 signaling prevents excessive activation of conventional T cells, limiting autoimmunity and tumor rejection; however, agonistic anti-CD27 antibodies can deplete or reprogram Tregs, thereby unleashing effector T cell responses against malignancies. In tumor microenvironments, blocking CD27 on Tregs has been shown to synergize with checkpoint inhibitors, promoting CD8+ T cell infiltration and tumor regression.^[32]^[33]^[34] Deficiency in CD27 profoundly impairs T cell immunity, as evidenced by studies in CD27-/- mice and human patients with CD27 mutations. In mice, CD27 knockout leads to defective primary and memory CD4+ and CD8+ T cell responses to viral infections such as influenza, resulting in reduced viral clearance and diminished long-term immunity. Human CD27 deficiency, often presenting as combined immunodeficiency, is associated with persistent Epstein-Barr virus viremia and impaired T cell-dependent vaccine responses, underscoring its role in generating effective humoral and cellular immunity to vaccinations.^[35]^[36]^[37]^[5]

Role in B Cell and Other Responses

CD27 plays a critical role in B cell biology by promoting key processes in humoral immunity. Upon ligation by CD70, CD27 signaling facilitates the differentiation of activated B cells into plasma cells, particularly in germinal centers where it upregulates survival factors such as Bcl-xL to support this transition.^[38] This interaction contributes to the generation of class-switched immunoglobulins during germinal center reactions, enabling efficient antibody production during immune responses.^[38] Furthermore, CD27 enhances affinity maturation within germinal centers by sustaining B cell proliferation and somatic hypermutation, as evidenced by its upregulation during the centroblast stage of B cell priming.^[5] In addition to differentiation, CD27 provides anti-apoptotic signals that enhance B cell survival, particularly for CD27+ memory B cells, which are long-lived and central to rapid recall responses upon re-exposure to antigens.^[39] These memory B cells, marked by CD27 expression, exhibit heightened responsiveness to stimuli like TLR9 ligands, leading to plasmablast differentiation and robust IgG secretion.^[5] Transient CD70 expression on activated B cells further amplifies this pathway, reinforcing CD27's role in maintaining humoral memory.^[40] Beyond B cells, CD27 ligation by CD70 boosts natural killer (NK) cell functions, enhancing cytotoxicity against target cells and promoting IFN-γ production to amplify innate immune responses.^[41] In NK T (NKT) cells, CD27 acts as a costimulatory receptor that supports activation and effector functions, contributing to bridging innate and adaptive immunity.^[42] Emerging evidence from mouse models indicates CD27 expression on hematopoietic stem cells enriches for populations with superior long-term repopulating activity, suggesting a potential role in stem cell maintenance.^[43] Recent studies have linked CD27 expression to antibody responses in infections, such as SARS-CoV-2, where increased CD27 gene expression post-vaccination correlates with higher anti-spike IgG titers at 1 and 3 months, highlighting its involvement in durable humoral immunity.^[44]

Signaling and Interactions

Downstream Signaling Pathways

Upon ligation by its ligand CD70, CD27, a member of the tumor necrosis factor receptor superfamily, primarily activates the NF-κB signaling pathway through recruitment of the adaptor proteins TRAF2 and TRAF5 to its intracellular tail. This interaction occurs via a specific PIQEDYR motif in the cytoplasmic domain, leading to the activation of NF-κB-inducing kinase (NIK) and subsequent nuclear translocation of the RelA (p65)/p50 heterodimer.^[45] The translocated NF-κB complex then drives transcription of anti-apoptotic genes, including Bcl-2 and c-FLIP, thereby promoting cell survival and preventing programmed cell death in activated lymphocytes.^[46] CD27 also engages the JNK/MAPK pathway, predominantly through TRAF2, which enhances TCR-induced JNK (MAPK8) activation in T cells. This signaling cascade fosters T cell proliferation and supports the production of cytokines such as IL-2, contributing to amplified immune responses during antigen encounter.^[47]^[45] In addition, CD27 costimulation activates the mTOR pathway, integrating with PI3K/Akt signaling to bolster metabolic reprogramming in T cells. This enhances protein translation via mTORC1-mediated phosphorylation of S6 kinase, while promoting glucose uptake and flux through glycolysis and the pentose phosphate pathway to support nucleotide synthesis and overall cellular fitness.^[31] CD27 signaling exhibits cross-talk that favors pro-survival pathways such as NF-κB and mTOR, which inhibit certain pro-apoptotic signals without direct involvement of caspases in the primary cascades. However, prolonged CD27 ligation can engage pro-apoptotic pathways, such as those mediated by the intracellular binder SIVA (see Key Protein Interactions subsection). This regulatory balance typically promotes survival during acute immune responses.^[46] The duration of CD27-mediated signaling remains transient, as CD70 expression is rapidly downregulated following receptor-ligand interaction through reverse signaling mechanisms that reduce CD70 transcription and surface levels. This feedback prevents chronic stimulation, thereby averting T cell exhaustion while sustaining effective immunity.^[48]

Key Protein Interactions

CD27 primarily interacts with members of the tumor necrosis factor receptor (TNFR)-associated factor (TRAF) family through specific motifs in its intracellular tail, facilitating downstream signaling events. TRAF2, TRAF3, and TRAF5 bind to the proline-rich region encompassing the PIQED motif (amino acids 246–250) in the cytoplasmic domain of CD27.^[45] These interactions are crucial for recruiting signaling adaptors that mediate NF-κB and JNK activation upon CD27 ligation, with dominant-negative forms of TRAF2 and TRAF5 blocking these pathways.^[45] TRAF3 exhibits weaker binding compared to TRAF2 and TRAF5, while TRAF6 does not associate directly with CD27.^[45] Another key interactor is SIVA1, a proapoptotic protein that binds to the intracellular domain of CD27 via its death domain homology region (SAH domain), which shares approximately 40% homology with death domains of FADD and RIP.^[10] This interaction promotes caspase-dependent apoptosis, particularly under conditions of prolonged CD27 ligation, such as chronic stimulation by CD70, leading to DNA fragmentation and cell death in T and B lymphocytes over 2 days.^[10]^[49] Overexpression of SIVA1 enhances CD27-induced apoptosis by 2–3-fold in cell lines like Jurkat and Ramos, underscoring its role in regulating lymphocyte homeostasis.^[10] CD27 lacks direct interactions with kinases and instead relies on TRAF recruitment to engage downstream kinases like NIK for signal propagation.^[45] Indirectly, CD27 clustering upon ligand binding influences co-stimulatory pathways involving CD28 and OX40, synergizing to enhance T cell proliferation, survival, and effector function during immune responses.^[50]^[51]

Soluble CD27

Generation and Measurement

Soluble CD27 (sCD27) is generated through proteolytic ectodomain shedding of the membrane-bound form, primarily mediated by matrix metalloproteinases (MMPs) at a membrane-proximal cleavage site. This process releases the extracellular domain, resulting in a soluble fragment with a molecular weight of approximately 28-32 kDa.^[52]^[53] Shedding of sCD27 is triggered by T cell activation, such as through T cell receptor (TCR) engagement or stimulation with phorbol esters, which downregulate surface expression and promote release. Levels of sCD27 are elevated in inflammatory conditions, reflecting heightened immune activation.^[52]^[54] The soluble form is measured primarily using enzyme-linked immunosorbent assay (ELISA) kits that detect sCD27 in serum or plasma, with normal levels typically below 1000 U/ml (mean around 215 U/ml in healthy individuals). Flow cytometry can quantify membrane-bound CD27 on cells, allowing differentiation from the soluble isoform in biological samples. Commercial ELISA assays, such as those from Sanquin or Invitrogen, provide standardized quantification for clinical and research applications.^[55]^[56]^[57] The released sCD27 retains partial capacity to bind its ligand CD70 but lacks the intracellular signaling domain of the membrane-bound receptor, rendering it unable to transduce signals. This form was first described in 1991 following observations of its release from activated T cells, and ELISA-based detection has since become standardized for monitoring immune activation in clinical settings.^[58]^[52]^[54]

Functional Implications

Soluble CD27 (sCD27) serves an immunoregulatory role by binding to CD70 with high affinity. Production of sCD27 requires ligation of membrane-bound CD27 with CD70, and sCD27 can further bind CD70 to induce reverse signaling in CD70-expressing cells, contributing to T cell activation and potentially enhancing tumor immunity.^[58] This mechanism influences downstream immune dynamics during antigen exposure. Elevated sCD27 levels are associated with pro-inflammatory states, particularly reflecting T cell activation and contributing to cytokine production in infections. For instance, in viral infections such as dengue, sCD27 release from activated B and T cells correlates with disease severity and inflammatory responses, including aspects of cytokine dysregulation akin to storms observed in severe cases.^[59] Similarly, during COVID-19, increased circulating sCD27 forms part of a broader "storm" of soluble immune checkpoints linked to hyperinflammation and poor prognosis, as it upregulates activation markers like IFN-γ on T cells.^[60]^[58] However, sCD27 is often elevated in autoimmune diseases like multiple sclerosis and systemic lupus erythematosus, serving more as a marker of ongoing T cell-driven inflammation than a direct suppressor.^[61]^[58] Recent studies highlight sCD27's prognostic value in immunotherapy resistance, where elevated baseline plasma levels predict poorer responses to anti-PD-1 monotherapy in metastatic melanoma, indicating T cell exhaustion in the tumor microenvironment. Specifically, 2025 analyses of patient cohorts showed high sCD27 associated with reduced progression-free and overall survival (HR=1.07 for PFS, p=0.0024), linked to enriched CD27 expression on exhausted CD8+ T cells from chronic CD27-CD70 interactions.^[62] This biomarker does not predict resistance to anti-PD-1 plus anti-CTLA-4 combinations, suggesting context-specific utility.^[62] Circulating sCD27 undergoes rapid clearance, primarily through proteolytic degradation by proteases.^[63]

Clinical and Pathological Roles

As a Biomarker in Diseases

Soluble CD27 (sCD27) serves as a valuable biomarker for assessing immune activation and disease progression in various non-oncologic conditions, reflecting T-cell burden and chronic inflammation through its release from activated lymphocytes.^[64] In infectious diseases, elevated sCD27 levels in plasma or cerebrospinal fluid indicate heightened T-cell activation and correlate with poorer outcomes. For instance, in HIV infection, increased sCD27 concentrations are associated with disease progression and persistent immune dysfunction, even in antiretroviral therapy-suppressed patients, serving as a marker of ongoing T-cell activation.^[65] Similarly, during Epstein-Barr virus (EBV) infections, sCD27 elevation reflects immune responses to viral persistence, aiding in monitoring T-cell involvement.^[66] In SARS-CoV-2 infection, higher sCD27 levels predict severe disease and mortality, particularly when combined with other soluble immune checkpoints, as demonstrated in studies as of 2024.^[67] In autoimmune disorders, sCD27 and membrane CD27 expression on immune subsets provide insights into chronic T- and B-cell activation. Rheumatoid arthritis (RA) patients exhibit elevated serum sCD27 levels, which correlate with disease activity scores and humoral immune responses, indicating sustained T-cell stimulation.^[68] In multiple sclerosis (MS), intrathecal sCD27 in cerebrospinal fluid acts as a sensitive biomarker of active T-cell-mediated inflammation and lesion activity, outperforming some traditional markers for monitoring relapse risk.^[69] Conversely, systemic lupus erythematosus (SLE) is characterized by reduced frequencies of CD27+ memory B cells and expansion of CD27-negative double-negative B cells, which serve as indicators of dysregulated B-cell maturation and disease flares.^[70] sCD27 also monitors immune responses in transplantation settings, where serum levels rise during episodes of graft rejection, reflecting alloreactive T-cell activation. In renal transplantation, particularly HLA antibody-incompatible cases, elevated pre- and post-transplant sCD27 predicts higher rejection risk, complementing other immune assays for patient stratification.^[71] Beyond these, sCD27 tracks immunomodulation in treated respiratory conditions; in azithromycin therapy for inflammatory airway diseases, declining sCD27 levels indicate reduced CD27hi T-cell expansion and type-1 effector responses, as shown in 2024 mechanistic studies.^[72] For enhanced diagnostic precision, combining sCD27 measurements with CD27 expression profiles on specific immune subsets, such as CD8+ T cells or regulatory B cells, improves specificity over solitary markers, enabling better differentiation of disease states. Additionally, elevated sCD27 levels have been observed in inflammatory bowel disease (IBD) flares, serving as a marker of T-cell-driven inflammation as of 2023.^[73]^[69]

Involvement in Cancer and Immunity

CD27, a member of the tumor necrosis factor receptor superfamily, plays a dual role in cancer, promoting tumor cell survival in certain malignancies while also influencing antitumor immune responses. In hematological cancers such as lymphomas and leukemias, CD27 expression on malignant cells enhances their survival and proliferation by activating prosurvival signaling pathways, including NF-κB, which contributes to disease progression. For instance, high CD27 mRNA levels in pro-B acute lymphoblastic leukemia correlate with poorer patient survival and higher relapse risk in some cohorts, though this may reflect tumor-promoting effects rather than immune context.^[74]^[75]^[6] Conversely, in solid tumors like non-small cell lung cancer (NSCLC) and renal cell carcinoma (RCC), CD70—the ligand for CD27—is frequently upregulated on tumor cells, leading to chronic signaling that exhausts infiltrating T cells and impairs antitumor immunity.^[74]^[6] The CD27-CD70 interaction facilitates immune evasion in the tumor microenvironment by inducing apoptosis in activated T cells through sustained costimulatory signaling, which shifts from activation to exhaustion and cell death. In RCC, tumor-derived CD70 engages CD27 on T cells, promoting regulatory T cell expansion and suppressing effector responses, thereby enabling tumor escape from immune surveillance. Additionally, elevated soluble CD27 (sCD27) levels in melanoma patients predict resistance to anti-PD-1 monotherapy, as demonstrated in a 2025 study where high plasma sCD27 correlated with poorer response rates and shorter progression-free survival, potentially due to systemic immune modulation. This prognostic association highlights CD27's role in checkpoint inhibitor resistance, with sCD27 serving as a biomarker for therapeutic escalation.^[76]^[6]^[77] Despite its tumor-promoting aspects, CD27 also supports antitumor immunity, particularly through CD27+ memory T cells, which are critical for sustaining vaccine-induced responses and long-term tumor control. These cells, marked by CD27 expression, exhibit enhanced persistence and effector function, contributing to the efficacy of cancer vaccines by orchestrating coordinated CD4+ and CD8+ T cell activity against poorly immunogenic tumors. A 2025 Duke University study further linked CD27 agonism to improved vaccine outcomes, showing that targeting CD27 in a breast cancer vaccine trial generated durable immune memory, with patients exhibiting long-term survival benefits up to 20 years post-vaccination. In immunotherapy contexts, CD27 costimulation enhances chimeric antigen receptor (CAR) T cell persistence and antitumor activity by upregulating antiapoptotic proteins like Bcl-xL, leading to better expansion and reduced exhaustion in vivo.^[78]^[79]^[29] In natural killer/T-cell (NK/T-cell) lymphoma, elevated soluble CD27 levels correlate with disease activity and immune evasion via CD70 on tumor cells, potentially contributing to aggressive outcomes and poor clinical prognosis.^[80]^[81]

Therapeutic Applications

Targeting Strategies

Targeting strategies for CD27 primarily focus on modulating its costimulatory role in T cell activation through agonistic or antagonistic approaches, aiming to enhance antitumor immunity or prevent immunosuppressive signaling via its ligand CD70. Agonistic monoclonal antibodies, such as varlilumab, bind to CD27 and induce receptor clustering, thereby promoting T cell proliferation and effector function by mimicking CD70 ligation. The potency of these antibodies is epitope-dependent, with specific binding sites enhancing downstream NF-κB and JNK signaling pathways for improved T cell costimulation, as demonstrated in preclinical models. Blocking strategies target the CD27-CD70 interaction to inhibit regulatory T cell expansion and T cell exhaustion, particularly in tumor microenvironments where CD70 is overexpressed on malignant cells. For instance, cusatuzumab, an anti-CD70 monoclonal antibody, blocks CD70 binding to CD27, thereby disrupting prosurvival signals in leukemia stem cells and enhancing antibody-dependent cellular cytotoxicity against CD70-positive tumors. Bispecific antibodies engaging CD70 on tumor cells and CD3 on T cells, such as those redirecting cytotoxic T lymphocytes, further exploit this axis by promoting targeted immune killing without direct CD27 modulation. Small molecule inhibitors of TNF receptor-associated factors (TRAFs), such as TRAF2 antagonists like liquidambaric lactone, offer a means to fine-tune CD27-mediated signaling by interfering with adaptor protein recruitment, potentially reducing excessive NF-κB activation while preserving costimulatory benefits; however, no direct small molecule modulators of CD27 itself have been approved for clinical use. Combination approaches integrate CD27 targeting with immune checkpoint inhibitors, such as pairing varlilumab with PD-1 blockers like nivolumab, to synergistically overcome T cell exhaustion and amplify antitumor responses by concurrently relieving inhibitory signals and boosting costimulation. Additionally, azithromycin has been shown to indirectly modulate the CD27 pathway by depleting CD27high effector T cells, thereby shifting immune responses away from pro-inflammatory Th1/Tc1 phenotypes in a TRAF-dependent manner. Key challenges in these strategies include the transient expression of CD70, which limits the duration of CD27-CD70 engagement and reduces therapeutic window for both agonism and blockade, as well as potential toxicity from CD27 overactivation leading to cytokine storms or unintended autoimmunity.

Clinical Trials and Developments

Varlilumab (CDX-1127), an agonistic anti-CD27 monoclonal antibody, has been evaluated in multiple phase I/II clinical trials for hematologic malignancies and solid tumors since 2015. In a phase I/II dose-escalation and expansion study in patients with advanced lymphoma (NCT01460134), varlilumab was well tolerated with no maximum tolerated dose identified, and transient elevations in proinflammatory cytokines such as MIP-1β, IL-12, and CXCL9 were observed, indicating immune activation. Modest antitumor activity was reported, including one complete response in Hodgkin lymphoma lasting over 33 months and stable disease in four patients for 4.5–14 months. In advanced solid tumors, a phase I/II trial combining varlilumab with nivolumab (NCT02335918) demonstrated increased intratumoral CD8+ and CD4+ T cells, particularly in ovarian cancer responders, along with transient chemokine increases like MIP-1β associated with improved progression-free survival; objective response rates varied by tumor type, reaching 12.5% in ovarian cancer and squamous cell carcinoma of the head and neck. Another combination trial with ipilimumab and CDX-1401 in unresectable stage III/IV melanoma (NCT02413827) was terminated early due to shifts in standard care, with no published efficacy outcomes. In November 2025, the multicenter RiVa trial reported results on varlilumab combined with rituximab in previously treated B-cell non-Hodgkin lymphoma, showing modest antitumor activity with immune activation through intratumoral stimulation, though overall response rates were low; the combination was well tolerated.^[82] Anti-CD70 therapies targeting the CD27-CD70 axis, such as cusatuzumab (ARGX-110), have shown promise in hematologic cancers. After Janssen discontinued development in 2021, argenx regained rights, and OncoVerity has continued advancement with expanded funding in 2024, expecting phase II interim results in the second half of 2025. In a phase II randomized dose-optimization study (CULMINATE, NCT04023526) for newly diagnosed acute myeloid leukemia ineligible for intensive chemotherapy, cusatuzumab combined with azacitidine yielded complete remission rates of 12% at 10 mg/kg and 27% at 20 mg/kg, with common grade 3+ adverse events including thrombocytopenia and anemia but no unexpected safety signals. In relapsed/refractory cutaneous T-cell lymphoma, a phase II expansion cohort reported an overall response rate of 23%, including one complete response and five partial responses, with higher rates (50%) in Sézary syndrome subtype.^[83] Emerging approaches include engineered T cells with CD27 costimulation or overexpression to enhance antitumor persistence. Preclinical and early clinical data support CD27-costimulated chimeric antigen receptor T cells outperforming CD28-costimulated variants in tumor control, with improved expansion and effector function in vitro. In a phase I trial of CD27-armored BCMA CAR T cells (CBG-002) for relapsed/refractory multiple myeloma, the therapy demonstrated safety and preliminary clinical activity, including objective responses in heavily pretreated patients. Soluble CD27 (sCD27) levels have been explored for patient stratification in immunotherapy trials, serving as a biomarker to predict resistance to anti-PD-1 monotherapy but not combinations with anti-CTLA-4, with higher baseline sCD27 correlating with poorer progression-free and overall survival in solid tumors under immune checkpoint inhibition; a March 2025 study in metastatic melanoma confirmed this differential predictive value, supporting escalation to combination therapy for high sCD27 patients.^[62] Despite these advances, CD27-targeted therapies remain limited to phase I/II trials, with no phase III studies completed or ongoing as of November 2025, highlighting the need for larger confirmatory trials. Safety profiles are generally favorable, but ongoing monitoring for potential autoimmunity risks associated with T-cell activation is emphasized in combination regimens. Future directions focus on integrating CD27 modulation with neoantigen vaccines and CAR T enhancements to address immunosuppressive tumor microenvironments in renal cell carcinoma and melanoma.

Genetic Variations

Pathogenic Mutations

Pathogenic mutations in the CD27 gene (TNFRSF7) are rare and typically inherited in an autosomal recessive manner, leading to CD27 deficiency and a combined immunodeficiency phenotype known as lymphoproliferative syndrome 2 (LPFS2; OMIM #615122).^[84] These mutations were first described in the early 2010s, with initial reports identifying nonsense variants such as the homozygous W8X mutation in patients presenting with persistent Epstein-Barr virus (EBV) viremia and hypogammaglobulinemia.^[85] Subsequent case series up to 2023 have documented additional variants in over 30 patients, often from consanguineous families, highlighting increased susceptibility to recurrent infections, EBV-driven lymphoproliferation, and malignancies like Hodgkin lymphoma.^[86]^[87] Loss-of-function missense mutations frequently affect cysteine residues in the cysteine-rich domains (CRDs) of the CD27 extracellular region, disrupting disulfide bonds essential for proper protein folding and membrane expression. For instance, the homozygous C53Y variant in CRD1, reported in eight individuals across multiple families, abolishes a critical disulfide bridge, resulting in markedly reduced surface CD27 expression on T and B cells. Similarly, the homozygous C96Y mutation in CRD2 impairs structural integrity, leading to absent or low CD27 levels and defective ligand (CD70) binding, as observed in four patients with severe EBV-associated complications. The R107C variant in CRD2, often occurring in compound heterozygosity (e.g., with W8X), further compromises ligand interaction and signaling, contributing to recurrent sinopulmonary infections and diminished T cell memory in affected cases.^[86] Nonsense and frameshift mutations cause premature termination or truncated proteins, exacerbating the loss of function. The W8X nonsense mutation, identified in early reports, produces a severely truncated protein lacking functional domains, while frameshift variants such as c.266_267del (p.S89Wfs*14) introduce stop codons that prevent full-length CD27 synthesis, as seen in two of 33 documented patients.^[85]^[86] These alterations collectively impair downstream NF-κB signaling through reduced recruitment of TRAF2 and TRAF5 adapters, leading to defective B cell class-switch recombination, low switched memory B cells, and poor antibody responses.^[86] Additionally, they hinder CD8+ T cell expansion and survival, particularly against EBV, resulting in low memory T cell formation and increased apoptosis under immune stress.^[86]

Polymorphisms and Functional Effects

Common genetic variants in the CD27 gene primarily consist of single nucleotide polymorphisms (SNPs) located in intronic and untranslated regions, which predominantly exert regulatory effects on gene expression rather than altering protein structure or function. For instance, the intronic SNP rs2534719 has a global minor allele frequency (MAF) of approximately 0.14, with notable population-specific variations such as lower frequencies in Japanese cohorts (MAF ≈ 0.026) compared to European groups (MAF ≈ 0.26). Similarly, the 3' untranslated region (3'UTR) variant rs1059501 exhibits a global MAF of about 0.49, with higher frequencies in East Asian populations like Koreans (MAF ≈ 0.45) versus lower rates in some South Asian groups. These regulatory positions suggest potential influences on mRNA stability and transcript levels without disrupting the CD27 protein's core signaling capabilities. Functional consequences of CD27 polymorphisms include associations with altered disease susceptibility through modulated immune responses. In a case-control study of 610 breast cancer patients and 617 controls from northern China, the rs3136550 CT genotype was linked to reduced breast cancer risk (odds ratio [OR] = 0.76, P = 0.03), while the rs2267966 AT genotype showed a similar protective effect (OR = 0.75, P = 0.02); these variants also correlated with lymph node metastasis and estrogen/progesterone receptor status, implying roles in immune surveillance of tumors. Although direct SNP-specific links to vaccine-induced T cell responses remain limited, CD27 signaling broadly enhances T cell proliferation and memory formation during antiviral immunity, and regulatory variants like those in introns could subtly influence vaccine efficacy by altering expression thresholds for costimulation. In autoimmunity, CD27 pathway dysregulation contributes to rheumatoid arthritis (RA) pathogenesis via excessive T cell activation, and while no CD27 SNPs are definitively causal in RA, nearby variants in chronic inflammatory disease GWAS datasets, including rs2266961, have been nominated for further evaluation in immune-mediated conditions like ankylosing spondylitis. Population differences in allele frequencies, such as elevated MAF for signaling-related variants in East Asians (e.g., rs1059501), may underlie varying immune response profiles across ethnic groups.^[89] Recent genome-wide association studies (GWAS) and Mendelian randomization (MR) analyses from 2023–2025 highlight CD27 variants' ties to infectious and oncogenic outcomes. A 2024 MR study using immune cell traits as exposures identified that elevated CD27 expression on memory B cells causally increases lung adenocarcinoma risk (OR = 1.047, 95% CI: 1.009–1.086, P = 0.01) and non-small cell lung cancer susceptibility, likely via expression quantitative trait loci (eQTLs) affecting B cell-mediated antitumor immunity; similar patterns were noted for NSCLC subtypes without direct SNP identification. For COVID-19, while no CD27 SNPs reached genome-wide significance in severity GWAS, MR evidence from 2025 linked critically ill COVID-19 genetic liability to altered CD27 expression on B cell subsets (e.g., CD27 on IgD+ CD38- unswitched memory B cells), suggesting indirect roles in hyperinflammatory responses and increased comorbidity risks like HER2-positive breast cancer through immune dysregulation. No major structural polymorphisms, such as large insertions or deletions, have been reported in CD27; variants are overwhelmingly regulatory, fine-tuning expression levels to modulate immune homeostasis without abolishing function.^[90]^[91]

References

[1]
939 - Gene ResultCD27 CD27 molecule [ (human)] - NCBI
Sep 9, 2025 · The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term ...
[2]
CD27 antigen - Homo sapiens (Human) | UniProtKB | UniProt
Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L ...
[3]
Entry - *186711 - CD27 ANTIGEN - OMIM
CD27 is a member of the tumor necrosis factor receptor (TFNR) superfamily. These receptors play an important role in cell growth and differentiation.
[4]
CD27 and CD70 in T cell and B cell activation - PubMed
The stimulatory effects of CD27 on B cell function appear to oppose those of CD70, which also has a signaling role. Targeting CD27-CD70 for therapy is ...
[5]
CD27 on human memory B cells–more than just a surface marker
Dec 12, 2022 · CD27 is a widely used cell surface marker for human memory B cells and is gradually upregulated during the germinal centre reaction.
[6]
The CD70-CD27 axis in oncology: the new kids on the block
Jan 6, 2022 · In this review on the CD70-CD27 axis, we focus on immunotherapeutic strategies aiming at eradicating the tumor cells by specifically targeting tumor-associated ...
[7]
The T cell activation antigen CD27 is a member of the nerve growth ...
In this study a cDNA-encoding CD27 was isolated by expression and immunoselection in COS cells. The predicted polypeptide was found to belong to a recently ...
[8]
CD27 Gene - GeneCards | CD27 Protein | CD27 Antibody
CD27 is a protein-coding gene, a TNF-receptor required for T cell immunity, and a costimulatory immune-checkpoint receptor on T, NK, and B cells.
[9]
Crystal structure of CD27 in complex with a neutralizing ... - NIH
Apr 26, 2017 · The crystal structure of human CD27 was determined at a resolution of 1.8 Å, revealing the assembly of the cysteine-rich domains.Missing: evolutionary mammals
[10]
CD27, a member of the tumor necrosis factor receptor family ... - PNAS
Using the yeast two-hybrid system, we have cloned a novel protein (Siva) that binds to the CD27 cytoplasmic tail. ... (1992). PubMed · Google Scholar. a ...
[11]
US9102737B2 - Human anti-CD27 antibodies, methods and uses
... residues 21-191 of the full-length protein can bind to CD70. The CD27 antigen cytoplasmic tail, residues 213-260, binds to the N-terminus of the SIVA protein ...
[12]
Structural delineation and phase-dependent activation of the ...
Mutagenesis of the N-glycosylation site N170Q decreases expression of CD70 and decreases binding to CD27 (Table 2). This site does not directly interact with ...
[13]
https://doi.org/10.1016/0092-8674(93)90133-b
[14]
CD27 and CD70 in T cell and B cell activation - ScienceDirect.com
The expression patterns of CD27 and CD70 in humans are very similar to those in mice, except that CD27 is expressed on primed B cells in higher frequency and ...
[15]
CD27 expression on Treg cells limits immune responses against ...
Regulatory T cells (Tregs) suppress immune responses and thus contribute to immune homeostasis. On the downside, Tregs also limit immune responses against ...
[16]
1341 CD27 an emerging immuno-oncology target at the cross-roads ...
Nov 7, 2022 · CD27 is a member of the TNF-Receptor superfamily expressed on CD4+ and CD8+ T cells, on NK and NKT cells and on B cells. It promotes T cell ...
[17]
Antibody Tumor Targeting Is Enhanced by CD27 Agonists ... - NIH
Nov 30, 2017 · CD27 is not expressed on macrophages, monocytes, or neutrophils (Figure S3E), ruling out direct activation of these cells by anti-CD27. We ...
[18]
Iterative Single-Cell Analyses Define the Transcriptome of the First ...
May 12, 2020 · Specific levels of Gata2, cKit, and CD27 expression define all functional in vivo repopulating HSCs, and, together with Ly6aGFP expression, CD27 ...
[19]
Modulation by IL-2 of CD70 and CD27 Expression on CD8+ T Cells
In this study, the interactions between IL-2, CD27, and its ligand CD70 and their effects on human T cell function were examined.
[20]
Participation of the CD27 antigen in the regulation of IL-2-activated ...
We found that some resting peripheral blood NK cells also express CD27. Furthermore, CD27 expression was up-regulated on NK cells stimulated by IL-2. The ...
[21]
The CD70-CD27 axis in oncology: the new kids on the block - PMC
Jan 6, 2022 · The strict expression patterns of CD70 and CD27 during normal physiology allow for regulation of immune responses via immune cell proliferation, ...
[22]
CD27 Promotes Survival of Activated T Cells and Complements ...
CD27 was found to rescue CD28−/− T cells from death at the onset of division, explaining its capacity to support a T cell response in absence of CD28. Keywords: ...
[23]
Targeting the T-Cell Co-stimulatory CD27/CD70 Pathway in Cancer ...
Jun 22, 2015 · In this review, we describe how CD27 co-stimulation impacts the T-cell response, with the purpose to illuminate how CD27 agonism can be exploited in cancer ...
[24]
Abstract B034: CD27 is a new promising T cell co-stimulatory target ...
Dec 1, 2023 · It enhances T cell activation, proliferation, and differentiation of effector and memory T cells after stimulation with its ligand, CD70.
[25]
CD27 Expression Promotes Long-Term Survival of Functional ...
Dec 6, 2004 · Our findings suggest that the CD27–CD70 interaction in HIV infection may provide CD27+ CD8+ T cells with a survival advantage and compensate for ...
[26]
The role of CD27 in anti-viral T-cell immunity - ScienceDirect.com
CD27 is a co-stimulatory receptor expressed on T-cells, B-cells and NK-cells. •. CD27-CD70 co-stimulation enhances primary, memory and recall T-cell responses.
[27]
The Role of CD27 in Anti-Viral T-cell Immunity - PubMed
CD27 is a co-stimulatory immune-checkpoint receptor, constitutively expressed on a broad range of T-cells (αβ and γδ), NK-cells and B-cells.Missing: 1655907 | Show results with:1655907
[28]
CD27 costimulation augments the survival and antitumor activity of ...
Jan 19, 2012 · CD27 costimulation enhances expansion, effector function, and survival of human CAR-T cells in vitro and augments human T-cell persistence and antitumor ...
[29]
Chronic CD27-CD70 costimulation promotes type 1-specific ...
Mar 12, 2023 · We conclude that CD27 may regulate the development of Th1 immunity in peripheral tissues as well as the subsequent switch of the effector response into long- ...Missing: conservation | Show results with:conservation<|control11|><|separator|>
[30]
CD27 costimulation supports metabolic fitness of CD4 + T cells by ...
May 5, 2025 · CD27 costimulation supported upregulation of glycolysis, the pentose phosphate pathway and the TCA cycle, increasing the use of glucose-derived ...
[31]
CD27 expression on Treg cells limits immune responses against ...
Aug 23, 2021 · We show that Treg-expressed CD27 prevents the breakdown of peripheral tolerance and limits anti-tumor immunity.
[32]
CD27-Mediated Regulatory T Cell Depletion and Effector T Cell ...
Abstract. CD27, a member of the TNFR superfamily, is constitutively expressed in most T cells and plays crucial roles in T cell effector functions.Missing: review | Show results with:review
[33]
Nasopharyngeal carcinoma cells promote regulatory T cell ... - Nature
Apr 6, 2023 · We reveal that nasopharyngeal carcinoma cells enhance development and suppressive activity of regulatory T cells via CD70-CD27 interaction.
[34]
CD27 is required for generation and long-term maintenance of T cell ...
Primary CD4+ and CD8+ T cell responses to influenza virus were impaired in CD27-/- mice. Effects of deleting the gene encoding CD27 were most profound on T cell ...
[35]
Reduced Naive CD4 T Cell Numbers and Impaired Induction of ...
In contrast, absence of CD27 expression in CD27-/- mice leads to reduced primary and secondary immune response to influenza infection [19]. Of note, CD4 and ...
[36]
Novel mutations in TNFRSF7/CD27
Apr 2, 2015 · Altered immunity to EBV appears to be the major complication of human CD27 deficiency. All mutations led to near-absent expression of T- and ...
[37]
https://www.jacionline.org/article/S0091-6749%2815%2900271-7/fulltext
[38]
CD27 and CD40 Inhibit p53-independent Mitochondrial Pathways in ...
Human CD27 is a B cell co-stimulatory molecule. The aim of this study was to compare the effects of CD27 and CD40 signals on BCR-mediated apoptosis of B cells.
[39]
Role of B cells in common variable immune deficiency - PMC
... IgG class switching and differentiation to antibody-secreting plasma cells. In this process, B cells gain CD27 expression and upregulate levels of TLR-7 and ...
[40]
CD27-mediated activation of murine NK cells - PubMed
Feb 15, 2000 · Although NK cell cytotoxicity was not triggered by anti-CD27 mAb or against CD70 transfectants, prestimulation via CD27 enhanced the cytotoxic ...
[41]
The Role of Natural Killer T Cells in Cancer—A Phenotypical and ...
Upon cytokine-mediated activation, type I NKT cells upregulate CD69 expression. Furthermore, CD4+, CD8+, and DN type I NKT cells express CD27, a costimulatory ...
[42]
Expression of CD27 on murine hematopoietic stem and progenitor ...
We show here that the CD27 protein is expressed by about 90% of cells in a purified HSC population. Interestingly, the CD27pos cells are enriched for cells ...Missing: embryonic | Show results with:embryonic
[43]
https://pubmed.ncbi.nlm.nih.gov/10714685/
[44]
https://doi.org/10.1016/j.genrep.2025.102196
[45]
CD27 sustains survival of CTLs in virus-infected nonlymphoid tissue ...
Dec 1, 2009 · Taken together, these data indicate that CD27 signaling induces IL-2 protein expression in CD8+ effector T cells, which is manifest in vivo ...
[46]
The TNF receptor family member CD27 signals to Jun N ... - PubMed
We show here that CD27 augments TCR-induced Jun N-terminal kinase (JNK) activity in primary murine lymph node T cells. To investigate how CD27 couples to JNK, ...Missing: TRAF3 | Show results with:TRAF3
[47]
CD27, a member of the tumor necrosis factor receptor family ... - NIH
CD27 is a glycosylated, type I transmembrane protein of about 55 kDa and exists as homodimers with a disulfide bridge linking the two monomers. The disulfide ...Missing: intracellular | Show results with:intracellular
[48]
CD70 is downregulated by interaction with CD27 - PMC
Engagement of the receptor CD27 by CD70 affects the magnitude and quality of T cell responses in a variety of infection models.Results · Cd70 Constitutively Recycles... · Cd70 Transcription But Not...Missing: stoichiometry | Show results with:stoichiometry
[49]
CD27-Mediated Apoptosis Is Dependent on Siva-Induced Caspase ...
Nov 16, 2005 · Since Siva is a death domain-containing proapoptotic protein identified as an intracellular ligand of CD27, we investigated its role in CD27- ...
[50]
OX40- and CD27-mediated co-stimulation synergize with anti-PD-L1 ...
OX40- and CD27-mediated co-stimulation synergize with anti-PD-L1 blockade by forcing exhausted CD8 + T cells to exit quiescence.
[51]
Molecular mechanisms of T cell co-stimulation and co-inhibition - PMC
CD27-, OX40- and DR3-mediated co-stimulation promotes proliferation and survival of both CD4+ and CD8+ effector T cells, whereas 4-1BB- and GITR-mediated co ...Box 1. The B7-Cd28... · Box 2. T Cell Receptor... · Cd28 And Icos Co-Stimulatory...<|control11|><|separator|>
[52]
The CD70–CD27 Axis in Cancer Immunotherapy - AACR Journals
The proapoptotic protein Siva and BAX are involved in CD27-mediated T-cell apoptosis via a caspase 3–dependent pathway. Siva also negatively regulates NF-κB ...
[53]
CD70 as a target in cancer immunotherapy - PubMed Central - NIH
Aug 15, 2025 · In hematological malignancies, tumor cells co-expressing CD27 and CD70 evade immune surveillance within the tumor microenvironment, thereby ...
[54]
A soluble form of the human T cell differentiation antigen CD27 is ...
Jul 1, 1991 · sCD27, purified from either culture supernatant or urine, has a molecular mass of 28 to 32 kDa and is, according to peptide mapping, ...
[55]
Metalloprotease inhibitors block release of soluble CD27 and ...
In this study, we evaluated whether protease inhibitors could inhibit the release of sCD27 from CLL cells and enhance T-cell activation mediated by CD27-CD70 ...
[56]
Plasma levels of soluble CD27: a simple marker to monitor immune ...
Plasma levels of soluble CD27 (sCD27) are elevated in diseases characterized by T cell activation and are used as a marker of immune activation.
[57]
High levels of serum soluble CD27 correlated with renal dysfunction
Of 138 patients with normal plasma creatinine, 42 showed high serum sCD27 levels (≥500 U/ml). ... normal values of 215±57 U/ml (n=27) (P value ≥0.001) ...
[58]
[PDF] Sanquin PeliKine Compact™ human soluble CD27 ELISA kit
This PeliKine compactTM human sCD27 ELISA kit [13] has been developed for fast, reproducible and specific quantification of human sCD27 in serum and in plasma, ...Missing: normal | Show results with:normal
[59]
Human CD27 (Soluble) Instant ELISA™ Kit (BMS286INST) - Invitrogen
The Human Soluble CD27 (Hu sCD27) ELISA quantitates Hu sCD27 in human serum, plasma, cell culture supernatants, or other body fluids.Missing: normal | Show results with:normal
[60]
Soluble CD27-pool in humans may contribute to T-cell activation ...
Here we report on the role of soluble CD27 (sCD27) in T-cell activation and its elevation in the serum of cancer patients after immunotherapy.Missing: esters inflammatory
[61]
Assessment of expression of TNF signaling proteins in Dengue ...
Castañeda et al. B cells naturally induced during dengue virus infection release soluble CD27, the plasma level of which is associated with severe forms of ...
[62]
Storm of soluble immune checkpoints associated with disease ...
Sep 7, 2020 · Here we provided evidence to link the storm of soluble immune checkpoints to COVID-19 progression. Consistently, a newly published study also ...
[63]
Agonistic anti‐CD27 antibody ameliorates EAE by suppressing IL ...
Jul 20, 2022 · ... autoimmune conditions [22]. Soluble CD27 is high in patients with multiple sclerosis (MS), systemic lupus erythematosus (SLE), and systemic ...
[64]
Soluble CD27 differentially predicts resistance to anti-PD1 alone but ...
Mar 27, 2025 · Recently, we reported that high plasma soluble CD27 (sCD27) levels reflect the intratumoral interaction of CD70-CD27 and dysfunctional T cells ...
[65]
Signaling via a CD27-TRAF2-SHP-1 axis during naïve T cell ...
... CD27 levels providing evidence for lysosomal and proteasomal degradation of internalized CD27 (Figure 2C). Levels of cell surface CD27 were gradually ...
[66]
Cerebrospinal fluid and plasma concentrations of the inflammatory ...
Jun 21, 2024 · Soluble CD27 (sCD27) is a potential biomarker for diseases involving immune dysfunction. As there is currently little data on cerebrospinal ...
[67]
Monitoring Circulating Immune Checkpoint Proteins as Predictors of ...
Soluble CD27 has been shown to directly contribute to the activation of T cells and B cells, leading to a state of hyperactivation [22]. Soluble CD80, on ...
[68]
Soluble CD27 as a predictive biomarker for intra‐tumoral CD70 ...
Jan 27, 2024 · In the present study, we show that CD70 is primarily expressed by tumor cells in NPC and that CD27‐positive lymphocytes infiltrate around tumor ...
[69]
Predicting survival in patients with SARS-CoV-2 based on cytokines ...
Nov 25, 2024 · Soluble immune checkpoints (sICs), which regulate T cell activity, have been reported as promising biomarkers of viral infections. Methods. In ...
[70]
Elevated serum soluble CD27 levels are associated with both ...
Jan 2, 2024 · Elevated serum soluble CD27 levels are associated with both disease activity and humoral immune activity in patients with rheumatoid arthritis.
[71]
Soluble CD27 is an intrathecal biomarker of T-cell-mediated lesion ...
Apr 12, 2024 · Our results demonstrate that soluble CD27 is a biomarker of disease activity, potentially informative for personalized treatment and monitoring of treatment ...
[72]
A new population of cells lacking expression of CD27 represents a ...
May 15, 2007 · Although CD27neg memory cells are relatively scarce in healthy subjects, they are substantially increased in systemic lupus erythematosus (SLE) ...
[73]
Soluble CD30 and Cd27 levels in patients undergoing HLA antibody ...
HLA antibody-incompatible transplantation has a higher risk of rejection when compared to standard renal transplantation. Soluble CD30 (sCD30) has been ...
[74]
Azithromycin targets the CD27 pathway to modulate CD27hi T ...
Aug 15, 2024 · Macrolide antibiotic azithromycin is widely used in clinical practice to treat respiratory tract infections and inflammatory diseases.
[75]
CD27 expression and its association with clinical outcome in ...
Jun 23, 2017 · Expression of CD27 protein and mRNA has been reported in B-cell lymphomas and adult T-cell leukemia/lymphoma. In acute myeloid leukemia CD27 has ...
[76]
CD27 expression on lymphoma cells prolongs survival. (AD) Kaplan ...
Using this threshold for the entire cohort, Kaplan-Meier analysis revealed that patients with high CD27 mRNA expression (.8.74) survived significantly longer ...
[77]
CD70 as a target in cancer immunotherapy - Frontiers
Aug 14, 2025 · CD70 also appears in solid tumors such as renal cell carcinoma, nasopharyngeal carcinoma, glioblastoma, melanoma, and carcinomas of the lung ...
[78]
Soluble CD27 differentially predicts resistance to anti-PD1 alone but ...
Mar 27, 2025 · Our results suggest that high plasma sCD27 levels predict poorer efficacy of anti-PD1 monotherapy in metastatic melanoma, justifying therapeutic escalation.
[79]
CD27 stimulation unveils the efficacy of linked class I/II peptide ...
Unfortunately, the efficacy of class I-restricted peptide vaccines has proven to be limited, with overall clinical response rates as low as 3%. Class II CD4+ T ...Missing: deficiency | Show results with:deficiency
[80]
Could a Cancer Vaccine Developed Long Ago Hold the Key to Long ...
Oct 28, 2025 · The findings, published in Science Immunology Oct. 28, suggest that targeting CD27 could dramatically improve the effectiveness of cancer ...
[81]
Expression of soluble CD27 in extranodal natural killer/T-cell ...
Feb 22, 2023 · The levels of serum sCD27 provided excellent diagnostic accuracy for discriminating patients with ENKL from healthy subjects, correlated ...
[82]
Quantitative Analysis of CD27/CD70 Protein Expression and ... - NIH
Aug 28, 2025 · Aberrant CD70 expression in tumors can facilitate immune evasion by promoting regulatory T cell (Treg) accumulation, contributing to ...
[83]
https://finance.yahoo.com/news/oncoverity-secures-extended-series-advance-190600841.html
[84]
https://omim.org/entry/615122
[85]
https://doi.org/10.1016/j.jaci.2011.11.013
[86]
Novel mutation in the CD27 gene in a patient presenting ... - PubMed
Feb 9, 2020 · CD27 deficiency is a rare primary immune deficiency which affects T cells, B cells and NK cells and is associated with hypogammaglobulinemia ...Missing: 2020-2023 | Show results with:2020-2023
[87]
CD27[gene] - SNP - NCBI
### Summary of Common SNPs in CD27 Gene (MAF > 0.01)
[88]
Association of CD27 and CD70 gene polymorphisms with risk of ...
Their abnormal expression can disturb the immune response and lead to an increased risk of cancer. This study aims to evaluate the associations between single ...
[89]
Causal association between immune cells and lung cancer risk
Jun 19, 2024 · Similarly, CD27 on memory B cells also showed increased risks for lung cancer subtypes (for LUAD, ORIVW =1.047; 95% CI: 1.009–1.086; P IVW =0. ...
[90]
Genetic Liability Critically Ill COVID-19 & HER2+ Breast Cancer
May 9, 2025 · Several studies have shown a close relationship between HER2-positive breast cancer and COVID-19 susceptibility and severity. 8–10 The ...