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CD43

CD43, also known as leukosialin or sialophorin, is a type I transmembrane sialomucin glycoprotein that serves as the major sialoglycoprotein on the surface of most hematopoietic cells, including thymocytes, T lymphocytes, granulocytes, monocytes, and dendritic cells, but is notably absent from erythrocytes and resting B cells.^[1]^[2] Its structure consists of an elongated, rod-like extracellular domain heavily modified with O-linked sialylated glycans—accounting for over 80% of its mass and extending up to 45 nm from the cell membrane—a single transmembrane helix, and a cytoplasmic tail of approximately 123 amino acids in humans that shares high homology with its murine counterpart and binds to ezrin-radixin-moesin (ERM) family proteins.^[1]^[2] Two major glycoforms, with molecular weights of 115 kDa and 130 kDa, arise from differences in glycosylation patterns.^[1] CD43 plays multifaceted roles in immune regulation, primarily functioning as an anti-adhesion molecule that promotes leukocyte repulsion and inhibits cell-cell interactions, thereby preventing excessive aggregation during immune responses.^[1] The extracellular domain acts as a physical barrier, sterically hindering close contact between cells, such as T cell-antigen presenting cell conjugates, while the cytoplasmic domain mediates intracellular signaling to negatively regulate T cell activation, proliferation, and interleukin-2 production independently of surface localization.^[2]^[1] Additionally, CD43 can switch to a pro-adhesive role by serving as a ligand for E-selectin, facilitating tethering and rolling of cutaneous lymphocyte antigen-positive (CLA+) T cells on endothelial cells during skin-homing immune responses.^[3] Through its interaction with ERM proteins, CD43 links the plasma membrane to the actin cytoskeleton, influencing cell migration, microvilli formation, and polarity in leukocytes.^[4] In clinical and pathological contexts, CD43 expression serves as a diagnostic marker for various hematologic malignancies, including T-cell lymphomas and aberrant B-cell populations such as those in mantle cell lymphoma, while aberrant expression or glycosylation of CD43 is observed in immunodeficiencies like Wiskott-Aldrich syndrome (caused by mutations in the WASP gene), leading to impaired cytoskeletal organization and platelet defects.^[1]^[5] Furthermore, CD43 exhibits antiviral properties by inhibiting HIV-1 entry into host cells through electrostatic repulsion mediated by its sialylated glycans, highlighting its broader role in innate immunity.^[1] Overall, CD43's dynamic regulation of adhesion, signaling, and migration underscores its essential function in maintaining immune homeostasis and orchestrating leukocyte behavior.^[6]

Overview

Definition and Nomenclature

CD43, also known as leukosialin, sialophorin, or galactoglycoprotein (GALGP), is a type I transmembrane sialomucin glycoprotein predominantly expressed on the surface of hematopoietic cells, particularly leukocytes.^[7]^[1]^[8] This protein is recognized within the cluster of differentiation (CD) system as CD43, a designation assigned during the Third International Workshop on Human Leukocyte Differentiation Antigens in 1986, reflecting its identification as a leukocyte surface marker.^[9] The gene encoding CD43 is designated SPN (sialophorin), a single-copy gene mapped to the short arm of human chromosome 16 at locus 16p11.2.^[10]^[11] This genomic location was established through somatic cell hybrid analysis and fluorescence in situ hybridization studies, confirming its position within a region associated with hematopoietic gene clusters.^[12] Biochemically, CD43 exhibits a molecular weight ranging from approximately 95 to 135 kDa, largely attributable to extensive post-translational modifications, including dense O-linked glycosylation.^[13] As a prototypical member of the sialomucin family, it is characterized by an abundance of sialic acid residues incorporated into its O-linked glycan chains, which impart a highly negatively charged and mucin-like extracellular domain.^[14]^[15] These features distinguish CD43 from other transmembrane proteins and underscore its role in modulating surface interactions on immune cells.^[9]

Discovery and History

CD43, also known as leukosialin or sialophorin, was first identified in 1984 as a major sialoglycoprotein on the surface of human leukocytes through biochemical analysis involving SDS-PAGE of lymphocyte membrane proteins. Researchers led by Eileen Remold-O'Donnell isolated and characterized this heavily glycosylated protein, noting its abundance on T lymphocytes and its distinct sialic acid content, which contributed to its negative charge and apparent molecular weight of approximately 115 kDa. This discovery highlighted CD43 as a prominent component of leukocyte plasma membranes, distinct from other known glycoproteins like CD45 (formerly T200). The nomenclature of the protein evolved rapidly in the mid-1980s. Initially referred to as gp95 based on its electrophoretic mobility, it was formally named leukosialin by Remold-O'Donnell and colleagues in their 1984 study, emphasizing its leukocyte-specific expression and sialic acid-rich structure. By 1986, independent work by Carlsson and Fukuda confirmed these findings and further detailed its O-linked glycosylation, reinforcing the leukosialin designation. The protein received its official cluster of differentiation (CD) assignment as CD43 during the Third International Workshop on Human Leukocyte Differentiation Antigens held in Oxford in 1986, standardizing its identification among immunologists.^[16] Milestone studies in the 1990s advanced the molecular understanding of CD43. In 1990, Shelley et al. cloned the human SPN gene encoding sialophorin (CD43), revealing its single-exon coding structure and chromosomal location on 16p11.2, which facilitated genetic analyses linking it to hematopoietic cell function. The 2000s brought functional insights through animal models; for instance, CD43 knockout mice generated in the mid-1990s demonstrated enhanced T-cell adhesion and activation, establishing its role in anti-adhesive mechanisms that prevent excessive leukocyte interactions. These findings, building on earlier work showing CD43's interference with integrin-mediated binding, underscored its regulatory importance in immune responses.^[17]^[18] Recent developments from 2023 to 2025 have illuminated CD43's involvement in immune evasion, particularly its sialylated form as a glyco-immune checkpoint. Studies have shown that hypersialylated CD43 on acute myeloid leukemia (AML) cells inhibits macrophage-mediated phagocytosis by engaging inhibitory sialic acid receptors like Siglec-7, allowing tumor escape from innate immunity. A 2025 study detailed how genetic ablation of CD43 or desialylation enhances phagocytic clearance of AML cells in preclinical models, positioning sialylated CD43 as a potential therapeutic target for glyco-immunotherapy.^[19]

Molecular Biology

Gene Characteristics

The SPN gene, which encodes the CD43 glycoprotein (also known as sialophorin or leukosialin), is located on the short arm of human chromosome 16 at the p11.2 cytogenetic band. The gene spans approximately 7.9 kb of genomic DNA, encompassing three exons that include both untranslated and coding regions.^[10]^[20] The primary coding sequence of the SPN gene consists of a 1,200 bp open reading frame, which translates into a 400-amino acid precursor protein comprising an extracellular domain, a transmembrane region, and a short cytoplasmic tail.^[7]^[21] The promoter region upstream of the SPN gene is TATA-less and lacks canonical CAAT boxes, aligning with features of housekeeping gene promoters through its GC-rich composition and reliance on initiator-like elements for transcription initiation. It includes multiple binding sites for the transcription factor Sp1, which binds to GC-box motifs and drives basal and cell-specific expression, particularly in hematopoietic lineages.^[22]^[23]^[24] The murine homolog of SPN resides on chromosome 17 and shares organizational similarities with the human gene. Evolutionary conservation is evident across mammalian species, with orthologs identified in over 80 species; the human and mouse proteins exhibit substantial sequence homology, notably in the conserved sialomucin domain critical for glycosylation and function.^[25]

Protein Structure

CD43, also known as sialophorin or leukosialin, is a type I transmembrane glycoprotein characterized by a single-pass transmembrane helix that anchors it in the plasma membrane. The mature protein comprises an extracellular domain spanning approximately 234 amino acids (residues 20–253), a 23-amino-acid transmembrane helix (residues 254–276), and a 124-amino-acid cytoplasmic tail (residues 277–400). This topology positions the bulk of the protein on the extracellular side, facilitating its role as a prominent cell surface protrusion. The overall molecular weight of the core polypeptide is around 40 kDa, but post-translational modifications significantly increase its size and alter its biophysical properties. The extracellular domain is a hallmark mucin-like region, densely packed with serine and threonine residues that serve as attachment points for extensive O-glycosylation. Up to 80 potential O-glycosylation sites have been identified, primarily featuring sialylated O-linked glycans such as core 1 structures (Galβ1-3GalNAcα-Ser/Thr) capped with sialic acid, along with some core 2 extensions. These carbohydrates constitute 70–90% of the molecule's weight, resulting in an apparent molecular mass of 115–135 kDa on SDS-PAGE and imparting a heavily negatively charged, hydrophilic character. The glycosylation pattern enforces a rigid, extended rod-like conformation, approximately 45 nm in length, as inferred from electron microscopy and hydrodynamic studies, which spaces the protein away from the cell surface and limits its flexibility. In contrast, the cytoplasmic domain lacks enzymatic activity but contains specific motifs for protein-protein interactions, including a positively charged juxtamembrane cluster (e.g., residues involving basic amino acids like lysine and arginine) that binds ezrin-radixin-moesin (ERM) family proteins. This interaction links CD43 to the actin cytoskeleton via the FERM domain of ERMs, as revealed by NMR structures of the tail peptide complexed with ezrin's FERM domain, showing conserved leucine residues stabilizing the binding interface. Additionally, the tail features multiple phosphorylation sites (e.g., serines at 291, 336, and 355) and a nuclear localization signal (residues 282–296), though these do not contribute to intrinsic catalytic function. Overall, the protein's architecture emphasizes its role as a structural scaffold rather than an active enzyme.

Expression and Regulation

Tissue Distribution

CD43 is predominantly expressed on hematopoietic cells, including most leukocytes except resting B cells, such as T cells, monocytes, granulocytes, natural killer cells, and platelets.^[1]^[26] It is absent from erythrocytes and non-hematopoietic tissues, restricting its distribution to cells of the blood and immune system lineages.^[27]^[28] During T cell development in the thymus, CD43 expression initiates early at the CD4⁻CD8⁻ double-negative stage of thymocyte maturation and is maintained at high levels through the double-positive and mature single-positive stages, where it is present on over 95% of thymocytes.^[29]^[1] In contrast, expression is absent on resting B cells but present on activated B cells and plasma cells, the terminally differentiated B cell subset.^[1]^[26] Quantitative analyses reveal the highest surface density on thymocytes, decreasing to moderate levels on peripheral mature T cells.^[30] The tissue distribution pattern of CD43 is highly conserved across species, exhibiting similar expression profiles in both humans and mice, as demonstrated by flow cytometry and immunohistochemistry techniques that consistently show enrichment in leukocyte populations and hematopoietic progenitors.^[28]^[31]

Expression Regulation

The expression of CD43, encoded by the SPN gene, is tightly controlled at multiple levels to ensure its restricted pattern in hematopoietic cells. Transcriptional regulation primarily occurs through a TATA-less promoter rich in GC nucleotides, lacking typical CAAT boxes but containing multiple Sp1 binding sites that drive basal expression in leukocytes. Sp1 transcription factor binds to GGGTGG motifs approximately 40 bp upstream of the transcription start site, facilitating recruitment of the transcriptional machinery and supporting high-level expression in hematopoietic lineages such as T cells, B cells, and myeloid cells. Repressors like hnRNP-K and Purα bind to pyrimidine-rich regions in the promoter, inhibiting transcription during leukocyte activation to modulate surface levels dynamically. Additionally, the tumor suppressor p53 downregulates CD43 at the transcriptional level by directly reducing promoter activity and mRNA stability in various cell types. Post-transcriptional mechanisms further fine-tune CD43 levels, including alternative splicing that generates at least four isoforms, with the major form predominant in leukocytes and minor variants potentially altering cytoplasmic signaling domains. The SPN mRNA undergoes processing that influences stability and translation efficiency, though specific miRNA interactions remain limited in characterization for hematopoietic contexts. Epigenetic modifications play a critical role in tissue-specific silencing. DNA hypermethylation of CpG islands in the 5'-regulatory region, particularly at sites like CCGG (-493 and +68 relative to the start site), represses transcription in non-hematopoietic tissues by blocking access to the promoter, while hypomethylation correlates with active expression in leukocytes and hematopoietic progenitors. Treatment with demethylating agents like 5-azacytidine induces CD43 expression in silenced cells, confirming methylation's repressive function. Histone acetylation, particularly of H3 and H4 at the promoter, enhances accessibility in activated leukocytes, promoting sustained expression during immune responses, though precise acetyltransferases involved require further elucidation. Developmentally, CD43 expression is induced early in lymphopoiesis, marking commitment to the hematopoietic lineage in pro-B and pro-T cells, and is maintained in mature leukocytes through ongoing protein synthesis and membrane turnover to support constant surface density. This regulation ensures high expression on most leukocytes while restricting it from non-hematopoietic cells.

Biological Functions

Adhesion and Repulsion

CD43 functions as a key anti-adhesive molecule on the surface of leukocytes, primarily through its extended, heavily sialylated extracellular domain that forms a negatively charged glycocalyx. This structure generates steric hindrance, physically repelling approaching cells and preventing close-range interactions that could lead to unwanted adhesion.^[32] The sialic acid-rich O-glycans on CD43 extend up to 45 nm from the plasma membrane, creating a repulsive barrier that inhibits homotypic aggregation among leukocytes, such as T cells clustering with one another.^[33] Additionally, this glycocalyx modulates integrin-dependent adhesion by masking or sterically obstructing binding sites. During leukocyte migration, CD43 prevents premature firm adhesion in the vasculature, enabling proper rolling along endothelial surfaces under shear flow. By maintaining cell separation through steric repulsion, CD43 inhibits excessive integrin activation early in the recruitment cascade, which would otherwise cause leukocytes to stick too readily and disrupt the transitional rolling phase mediated by selectins.^[34] This anti-adhesive function facilitates subsequent steps, including chemokine-induced activation and diapedesis, as shedding or redistribution of CD43 upon stimulation allows for timely progression to transmigration.^[35] Experimental evidence from CD43 knockout mice demonstrates these effects: deficient T cells exhibit enhanced homotypic clustering and increased adhesion to immobilized ligands, leading to hyperresponsive aggregation and altered migratory dynamics, including modified thymic emigration patterns. CD43 is anchored to the actin cytoskeleton via ERM proteins, enabling its polarized redistribution to support these adhesion-modulating roles.^[32]

Immune Cell Signaling

CD43's cytoplasmic tail plays a critical role in transducing signals within immune cells, particularly upon ligand engagement. The tail contains a conserved tyrosine residue that undergoes phosphorylation by Src family kinases, such as Fyn, following CD43 crosslinking or activation.^[36] This phosphorylation event facilitates the recruitment of ezrin-radixin-moesin (ERM) proteins to the cytoplasmic domain via a specific KRR tripeptide motif.^[37] ERM proteins, in turn, link CD43 to the actin cytoskeleton and activate Rho GTPases, promoting cytoskeletal remodeling essential for cell migration and polarity in leukocytes.^[38] In T cells, CD43 acts primarily as a negative regulator of activation. Crosslinking of CD43 or its failure to be excluded from the immunological synapse inhibits T cell receptor (TCR) signaling through intracellular mechanisms involving the cytoplasmic tail.^[2] This inhibition reduces interleukin-2 (IL-2) production by approximately 40% in affected cells, as observed when ERM binding is disrupted, limiting optimal T cell responses.^[39] Consequently, CD43 contributes to T cell anergy by dampening proliferative responses and cytokine secretion, thereby maintaining immune homeostasis. CD43 modulates phagocytosis in macrophages, particularly in the context of tumor immune evasion. Sialylated forms of CD43 on tumor cells interact with Siglec-7 on macrophages, delivering inhibitory signals through ITIM motifs in Siglec-7 that suppress engulfment.^[40] This glycan-dependent binding promotes tumor cell survival by inhibiting macrophage-mediated clearance, highlighting CD43's role in immune suppression. In B cells, CD43 expression enhances the signaling threshold of the B cell receptor (BCR), helping to prevent excessive activation that could lead to autoimmunity. CD43-positive B cells show increased proliferation in response to stimuli like TPA or anti-CD40 antibodies, indicating a modulatory function in activation.^[41] In mouse models, CD43 deficiency results in hyperactive B cells with enhanced responses to antigens, contributing to dysregulated humoral immunity.^[42]

Protein Interactions

Key Binding Partners

CD43 engages in several key non-glycan-mediated protein-protein interactions, primarily through its cytoplasmic domain, which plays a critical role in linking the protein to intracellular signaling and structural components. One prominent set of binding partners is the ezrin-radixin-moesin (ERM) family of cytoskeletal linkers. CD43 binds ERM proteins (ezrin, radixin, and moesin) via a cluster of basic residues in its juxtamembrane cytoplasmic tail, facilitating the connection to the actin cytoskeleton and contributing to T-cell polarity and migration.^[43] This interaction has been demonstrated through co-immunoprecipitation and colocalization studies, where the cytoplasmic domain of CD43 specifically precipitates ERM proteins, enabling CD43 redistribution to the uropod during T-cell polarization.^[44] Furthermore, ERM binding regulates CD43 phosphorylation, which in turn influences T-cell trafficking.^[37] CD43 also associates with integrins, notably LFA-1 (CD11a/CD18), to modulate leukocyte adhesion and motility. This interaction fine-tunes LFA-1 affinity states, allowing CD43 to either enhance or inhibit adhesiveness depending on activation signals, thereby supporting efficient T-cell migration across endothelial barriers.^[45] Experimental evidence from co-immunoprecipitation and adhesion assays shows that CD43 directly interacts with LFA-1 and the auxiliary protein CD147, promoting conformational changes in LFA-1 that regulate leukocyte-endothelium interactions without relying on glycosylation.^[46]

Glycosylation-Dependent Interactions

CD43's extensive O-linked glycosylation, particularly its sialylated forms, plays a pivotal role in mediating interactions that modulate immune responses and cellular processes. The protein's mucin-like domain bears numerous O-glycans, including those capped with α2-3-linked sialic acids such as N-acetylneuraminic acid (Neu5Ac), which serve as ligands for sialic acid-binding immunoglobulin-like lectins (Siglecs). For instance, sialylated O-glycans on CD43 engage Siglec-7 on natural killer (NK) cells, recruiting the receptor to the immune synapse and delivering inhibitory signals that suppress NK-mediated cytotoxicity against leukemia cells; blockade of this interaction enhances tumor cell lysis by 40-50%.^[40] Similarly, in erythroblastic islands, CD43 on early erythroblasts interacts with Siglec-1 (CD169) on central macrophages to facilitate island formation and support erythroid differentiation, though this binding relies on core O-glycosylation rather than sialic acid residues, as demonstrated by unchanged affinity following sialidase treatment.^[47] This sialylation is regulated by sialyltransferases such as ST3GAL1 and ST3GAL2, which redundantly add α2-3-linked Neu5Ac to core 1 O-glycans on CD43 in hematopoietic cells.^[48] Beyond Siglecs, nonsialylated core 1 O-glycans on CD43 can bind galectins, influencing tumor progression. For example, these glycans interact with galectin-1 on activated immune cells, inducing T-cell apoptosis and potentially aiding tumor immune escape, while in cancer cells, such galectin binding supports altered adhesion and metastatic potential by modulating cell surface lattice formation.^[49] In viral pathogenesis, CD43's glycosylation facilitates incorporation into viral envelopes via lipid raft association. A 2025 study revealed that phosphatidylinositol 4,5-bisphosphate (PIP2)-enriched lipid rafts at the plasma membrane promote CD43 clustering with HIV-1 Gag proteins, enabling efficient recruitment of CD43 alongside PSGL-1 and CD44 into nascent virions; PIP2 depletion reduces CD43 incorporation fivefold, impairing viral assembly and attachment.^[50] This mechanism highlights CD43's glycosylation-dependent role in envelopment, analogous to processes in other retroviruses like HTLV-1, where raft-mediated sorting aids particle formation.^[51]

Clinical Significance

Role in Diseases

CD43 plays a significant role in various diseases, particularly through its aberrant expression, glycosylation patterns, and functional dysregulation, which contribute to pathological processes such as immune evasion and altered cell interactions. In hematological malignancies, CD43 is frequently overexpressed on leukemic blasts, with hypersialylation enhancing its anti-phagocytic properties. For instance, in acute myeloid leukemia (AML), sialylated CD43 acts as a glyco-immune checkpoint that inhibits macrophage-mediated phagocytosis, thereby promoting tumor cell survival and immune evasion. This hypersialylated form is a major effector of O-linked glycosylation pathways in AML cells, and its expression is observed across diverse AML subtypes, serving as a potential prognostic indicator in a substantial proportion of cases where it correlates with adverse outcomes. Recent studies have shown that the glycosyltransferase ST3GAL4 drives this hypersialylation by synthesizing Siglec-9 ligands on CD43, further suppressing phagocytosis; inhibiting ST3GAL4 enhances immune clearance in preclinical models.^[52] Similarly, in T-cell acute lymphoblastic leukemia (T-ALL), CD43 is expressed on the majority of leukemic cells, where specific glycosylated epitopes facilitate immune escape and are targeted by therapeutic antibodies. In autoimmune disorders, alterations in CD43 expression and function contribute to dysregulated immune responses. Deficiency or defective expression of CD43 on lymphocytes is a hallmark of Wiskott-Aldrich syndrome (WAS), an X-linked immunodeficiency characterized by eczema, thrombocytopenia, and recurrent infections, leading to WAS-like phenotypes with impaired T-cell adhesion and signaling.^[53] In systemic lupus erythematosus (SLE), CD43 expression is abnormally reduced on T cells, inversely associated with autoantibody levels such as IgG, which may impair immune regulation and contribute to autoimmunity.^[54] CD43 also influences infectious diseases by modulating viral propagation through glycosylation changes. In human T-cell leukemia virus type 1 (HTLV-1) infection, the virus upregulates O-glycosylation of CD43 on infected T cells, which reduces cell adhesion to prevent nonspecific contacts while facilitating targeted cell-to-cell viral spread via virological synapses.^[55] For human immunodeficiency virus (HIV), CD43 is incorporated into virions during assembly, where its presence on the viral envelope modulates infectivity by interfering with virus-cell attachment and transinfection, potentially limiting but also influencing viral dissemination in certain contexts.^[56]

Therapeutic and Diagnostic Potential

CD43 has emerged as a valuable marker in diagnostic flow cytometry for leukemia subtyping, particularly in distinguishing hairy cell leukemia (HCL) from other B-cell neoplasms. In HCL, CD43 expression is typically absent or dimly positive, contrasting with its brighter expression in chronic lymphocytic leukemia (CLL), allowing for differential diagnosis through multiparametric panels that include CD19, CD20, CD11c, and CD103.^[57] Similarly, CD43 co-expression with CD200 enhances the separation of CLL from other non-Hodgkin lymphomas, improving diagnostic accuracy in peripheral blood and bone marrow samples.^[58] Aberrant CD43 expression patterns also aid in identifying HCL variants, where brighter CD43 intensity correlates with distinct clinicopathological features.^[59] Sialylation levels of CD43 serve as a potential biomarker for acute myeloid leukemia (AML) progression, with hypersialylated forms (CD43s) overexpressed on leukemic blasts compared to normal myeloid cells. Detection of CD43s via specific monoclonal antibodies like AT1413 has shown utility in identifying high-risk AML subsets, correlating with poorer prognosis and resistance to therapy.^[60] Although lectin arrays have been explored for broader glycan profiling in AML, CD43 sialylation assessment primarily relies on flow cytometry or immunohistochemistry to monitor disease dynamics and treatment response.^[61] Therapeutic targeting of CD43 focuses on antibodies that recognize sialylated epitopes to promote immune-mediated clearance. The monoclonal antibody AT1413, which binds the hypersialylated CD43s epitope, enhances phagocytosis of AML cells by macrophages in preclinical models, reducing tumor burden without significant toxicity to normal hematopoietic cells.^[60] Similarly, sialidase enzymes engineered to desialylate CD43 have demonstrated immune activation by exposing pro-phagocytic signals, with fusion proteins combining sialidases and bispecific T-cell engagers showing efficacy against solid tumors in mouse models. A Phase 1/2 trial (NCT05259696) is evaluating a bi-sialidase fusion protein (E-602) in combination with anti-PD-1 for advanced solid tumors to enhance phagocytosis via desialylation, with preliminary data as of 2024 showing tolerable safety.^[62] In immunotherapy approaches, T-cell redirecting strategies against hypersialylated CD43 offer promise for AML treatment. Bispecific antibodies linking AT1413 to CD3 retarget cytotoxic T cells to CD43s-expressing blasts, inducing potent lysis in vitro and in xenograft models while sparing non-malignant cells due to epitope specificity.^[63] Although full CAR-T constructs targeting CD43 remain preclinical, these bispecific formats show preclinical efficacy in AML models by overcoming hypersialylation-mediated immune evasion.^[64] Challenges in CD43-targeted therapies stem from its broad expression on normal leukocytes, potentially leading to off-target effects like cytopenias. Specificity is improved by focusing on cancer-associated glycoforms like CD43s, but clinical translation requires careful epitope selection to minimize toxicity.^[65]

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[41]
Ezrin/Radixin/Moesin (ERM) Proteins Bind to a Positively Charged ...
Ezrin/Radixin/Moesin (ERM) proteins bind to a positively charged amino acid cluster in the juxta-membrane cytoplasmic domain of CD44, CD43, and ICAM-2.Missing: motifs | Show results with:motifs
[42]
CD43 Interacts With Moesin and Ezrin and Regulates Its ...
The interaction of moesin and ezrin with CD43 was specifically mediated by the cytoplasmic domain of CD43, as shown by precipitation of both ERM proteins with a ...
[43]
LFA-1-mediated leukocyte adhesion regulated by interaction of ...
It is well known that the sialoglycoprotein CD43 can influence LFA-1-mediated cell adhesion in an either anti- or pro-adhesive manner through mechanisms not ...
[44]
LFA-1-mediated leukocyte adhesion regulated by interaction of ...
It is well known that the sialoglycoprotein CD43 can influence LFA-1-mediated cell adhesion in an either anti- or pro-adhesive manner through mechanisms not ...Missing: reduction assay
[45]
T‐helper cell regulation of CD45 phosphatase activity by galectin‐1 ...
Jun 2, 2022 · We propose activated Th cells from the microenvironment upregulate the CD45 phosphatase activity of CLL cells via galectin‐1 and/or CD43. This ...
[46]
Galectin-1 binds different CD43 glycoforms to cluster ... - PubMed
Oct 15, 2006 · Galectin-1 kills immature thymocytes and activated peripheral T cells by binding to glycans on T cell glycoproteins including CD7, CD45, and CD43.
[47]
CD169-CD43 interaction is involved in erythroblastic island ...
Aug 1, 2023 · CD169 and CD43 interact to mediate erythroblastic island (EBI) formation. CD43 is a counter receptor for CD169 and a novel indicator of ...
[48]
Sialylated CD43 is a glyco-immune checkpoint for macrophage phagocytosis
**Summary of Sialylated CD43's Role in Macrophage Phagocytosis Inhibition**
[49]
Overlapping and unique substrate specificities of ST3GAL1 and 2 ...
Jul 6, 2022 · Taken together, these data demonstrate that ST3GAL1 and ST3GAL2 work redundantly for O-glycan sialylation on CD34 and CD43 in HPCs and that when ...
[50]
Altered Tumor-Cell Glycosylation Promotes Metastasis - Frontiers
An increase in α2,6-sialylation in tumors is usually attributed to the upregulation of ST6Gal1 sialyltransferase that is primarily active on N-linked glycans ( ...
[51]
PIP2 promotes the incorporation of CD43, PSGL-1, and CD44 into ...
Apr 4, 2025 · We found that depletion of cellular PIP 2 blocks incorporation of CD43, PSGL-1, and CD44 into HIV-1 particles.Missing: HTLV- | Show results with:HTLV-
[52]
New Insights into HTLV-1 Particle Structure, Assembly, and Gag ...
Gag proteins associate with raft microdomains of the inner leaflet of the plasma membrane [10,11]. The Gag polyprotein is a primary driving force for assembly ...Missing: CD43 | Show results with:CD43
[53]
CD43 Molecule Defective in Wiskott-Aldrich Syndrome Binds ICAM-1
Nov 21, 1991 · Here we report evidence that intercellular adhesion molecule-1 (ICAM-1, or CD54), is a ligand for CD43.
[54]
Abnormal expression of CD43 in patients with systemic lupus ...
Compared to healthy controls, both CD43 mRNA and protein expressions were reduced in T cells from patients with SLE, and were inversely correlated with IgG.Missing: sialylation | Show results with:sialylation
[55]
The Role of B Cell and T Cell Glycosylation in Systemic Lupus ...
Jan 3, 2023 · In this review, we summarize findings on glycosylation alterations in SLE and how they relate to immune system defects and their clinical manifestations.
[56]
Virion-incorporated PSGL-1 and CD43 inhibit both cell-free ... - PNAS
Mar 19, 2020 · Both PSGL-1 and CD43 are type-1 transmembrane proteins and sialomucins that are highly glycosylated with sialylated O-linked glycans (23–25).
[57]
[CD43 in B-cell chronic lymphocytic leukemia] - PubMed
Expression of CD43 is independent from expression CD5 and CD6 and diminishes during tumor mass increasing, what can depended from releases exocellular domains ...
[58]
Differential Expression of CD43, CD81, and CD200 in Classic ... - NIH
Hairy cell leukemia (HCL) and hairy cell leukemia variant (HCLv) are rare diseases with overlapping clinicopathological features.Flow Cytometric... · Fig. 1 · Literature CitedMissing: applications subtyping
[59]
Determination of CD43 and CD200 surface expression improves ...
Jul 27, 2020 · Diffuse large B-cell lymphoma (DLBCL) with CD43 expression are associated with a poor prognosis (Ma, Zheng, Yuan, Jiang, & Wang, 2015; Mitrovic ...2 Patients And Methods · 2.2. 1 Fluorochrome Staining... · 3 Results
[60]
Immunophenotypic Analysis of Hairy Cell Leukemia (HCL) and ...
Feb 18, 2022 · The aim of this retrospective study was to describe the immunophenotypic profile of HCL and HCL-like disorders using 13 markers and to assess the added value ...
[61]
Patient-derived antibody recognizes a unique CD43 epitope ... - PMC
Aug 18, 2017 · AT1413 is a monoclonal antibody isolated from a cured patient with AML that recognizes CD43s, a novel epitope expressed by AML and MDS ...
[62]
The sweet symphony of N-glycans in myeloid malignancies - Frontiers
Jul 7, 2024 · We review the current literature on (1) N-glycosylation of major hematopoietic growth receptors found mutated in myeloid malignancies, (2) chemoresistance.
[63]
A Chemo-enzymatically Linked Bispecific Antibody Retargets T ...
These findings offer preclinical evidence for the therapeutic potential of a bTCE antibody that targets a sialylated epitope on CD43 in AML.
[64]
A Chemo-enzymatically Linked Bispecific Antibody Retargets T ...
Jul 1, 2019 · AT1413 binds CD43s, a unique sialylated epitope on CD43, which is weakly expressed on normal myeloid cells and overexpressed on AML cells.Missing: CAR- | Show results with:CAR-
[65]
The universe of galectin-binding partners and their functions in ...
Through protein–glycan or protein–protein interactions, these endogenous lectins can shape the initiation, perpetuation, and resolution of these processes, ...
[66]
Cancer-Associated CD43 Glycoforms as Target of Immunotherapy
CD43 is a sialoglycosylated membrane protein that is involved in cell proliferation and differentiation. CD43 glycoforms that are recognized by the UN1 ...In Vivo Tumor Growth... · Competition Assay Of Un1 Mab... · Results<|control11|><|separator|>
[67]
The glycosyltransferase ST3GAL4 drives immune evasion in acute ...
Nov 17, 2024 · This work reveals a novel axis of immune evasion and implicates ST3GAL4 as a possible target for immunotherapy in AML.