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Clarifying agent

A clarifying agent, also known as a fining agent, is a processing aid employed in the food and beverage industry to eliminate suspended solids, haze, and impurities from liquids, thereby improving clarity, stability, and overall product appeal.^[1] These agents operate through mechanisms such as flocculation—where particles aggregate into larger, separable masses—adsorption of unwanted compounds like proteins and polyphenols, enzymatic degradation of pectins, or ionic bonding to precipitate turbidity-causing elements.^[2]^[3]

Historical Context

Fining, the traditional term for clarification, is an ancient practice dating back to Roman times, where substances like egg whites and blood were used to clear wine by binding to suspended particles.^[4] Over centuries, techniques evolved, with milk punches employing casein for clarification documented as early as the 18th century in beverages.^[5] Modern synthetic and plant-based agents emerged in the 20th century to improve efficiency and accommodate dietary preferences. Primarily utilized in the production of wines, beers, fruit juices (such as apple, grape, and gooseberry), and other beverages, clarifying agents prevent sedimentation, reduce astringency from tannins, and enhance visual and sensory qualities without filtration alone.^[2]^[3] Common types include protein-based options like gelatin and casein, which carry positive charges to bind negatively charged particles; mineral-based agents such as bentonite clay, which adsorbs proteins; and enzymatic agents like pectinase, which hydrolyze cell wall components to release and clarify juices.^[2]^[3] Other notable examples encompass chitosan (derived from crustacean shells), polyvinylpyrrolidone (PVPP) for polyphenol removal, and silica-based colloids for fine haze elimination.^[3] While highly effective—often achieving up to 95% transmittance in treated juices and extending refrigerated shelf life to 49 days—the application of clarifying agents requires careful dosing (e.g., 1–2 mg/mL for gelatin or bentonite) to minimize nutrient losses, such as vitamin C or antioxidants, and avoid over-fining that could strip desirable flavors.^[2]^[3] Regulatory bodies like the FDA classify them as processing aids, ensuring their use aligns with food safety standards.^[1] In recent years, the industry has increasingly adopted natural and vegan alternatives to meet consumer demand for plant-based products.^[6]

Introduction

Definition

A clarifying agent is a substance or material added to liquids to remove suspended solids, haze, or impurities by promoting the aggregation and settling of particles, thereby improving overall clarity and purity.^[7] These agents function primarily by destabilizing colloidal suspensions, where fine particles are initially stabilized by electrostatic repulsion; the agents neutralize surface charges, reducing this repulsion and enabling particles to collide and form larger aggregates.^[8] This process enhances the liquid's visual and functional quality without altering its core composition significantly.^[2] The key property of clarifying agents lies in their ability to induce flocculation, a mechanism where destabilized fine particles coalesce into larger clumps known as flocs.^[9] These flocs are denser and more readily separated from the liquid through methods such as sedimentation, filtration, or centrifugation, allowing for efficient removal of turbidity-causing materials.^[10] Flocculation typically occurs in stages: initial charge neutralization followed by bridging or sweep coagulation, resulting in settleable aggregates that clarify the suspension over time, often within minutes to hours depending on conditions like pH and dosage.^[9] In practice, clarifying agents encompass general categories such as fining agents employed in winemaking to remove haze or coagulants utilized in water treatment to eliminate suspended matter, serving essential roles in achieving transparent liquids for various industrial and consumer applications.^[11]

Historical Context

The use of clarifying agents dates back to ancient civilizations, where natural substances were employed to purify water and clarify beverages. In ancient Egypt around 1500 BCE, records indicate the application of alum, a naturally occurring mineral, to coagulate suspended particles in cloudy water, facilitating sedimentation and improving clarity for drinking purposes.^[12] This early coagulation technique marked one of the first documented uses of chemical agents in water treatment, relying on alum's ability to aggregate impurities for easier removal.^[13] While direct evidence for winemaking clarification in Egypt is sparse, ancient practices across the Near East and Mediterranean involved natural fining agents like resins to stabilize and clear fermented beverages, laying foundational methods for later developments.^[14] During the Middle Ages in Europe, winemaking and brewing techniques advanced with the incorporation of animal-derived fining agents, such as gelatin from animal collagen and isinglass from fish swim bladders, to remove haze and improve beverage stability. These organic materials were introduced to European viticulture as monasteries and trade routes facilitated knowledge exchange, enhancing clarification processes in both wine and beer production. By the post-Industrial Revolution era in the 19th century, industrial-scale production of gelatin emerged, enabling its widespread adoption as a clarifying agent in food processing, including wine fining to bind tannins and proteins.^[15] This period saw a shift toward more efficient, large-scale applications, with gelatin's protein structure proving effective for removing suspended solids in juices and wines.^[16] A key milestone in municipal water purification occurred in the 1880s, when alum was systematically adopted in European water works, such as in Groningen, Holland, where it was integrated into filtration systems to treat surface water for public supply.^[17] This innovation expanded coagulation to urban scales, addressing turbidity in rivers like the Seine, where alum had been experimentally used since the 1820s but gained prominence in the late 19th century. The 20th century brought a synthetic shift, particularly post-World War II, as chemical engineering advanced polymers for water treatment; polyacrylamide, first commercialized in the 1950s, revolutionized flocculation by forming larger aggregates for sedimentation in wastewater and drinking water processes.^[18] Bentonite clay also gained traction in winemaking from the 1930s, valued for its protein-binding properties in clarifying white wines.^[19] The 1970s marked a regulatory turning point with the enactment of the Safe Drinking Water Act in 1974, which established national standards for contaminants and prompted scrutiny of treatment chemicals like coagulants, leading to the development of safer alternatives to reduce risks such as disinfection byproducts and heavy metals.^[20] These regulations, enforced by the Environmental Protection Agency, encouraged innovations in polymer-based flocculants and non-toxic agents, balancing efficacy with environmental and health safety in both water treatment and beverage industries.^[21]

Types of Clarifying Agents

Natural Agents

Natural clarifying agents are derived from biological or mineral sources and have been employed in food processing for centuries due to their compatibility with organic production standards. These agents typically work by interacting with suspended particles to promote clarity without introducing synthetic residues. Protein-based agents include gelatin, which is extracted from animal collagen found in skins, bones, and connective tissues. Gelatin adsorbs tannins and haze-forming proteins, particularly in wine production.^[22] Another example is isinglass, derived from the dried swim bladders of fish such as sturgeon, which similarly targets tannins and proteins through adsorption.^[23] Casein, a milk-derived protein, is commonly used in beer fining to achieve brightness.^[24] Mineral-based agents encompass bentonite clay, primarily composed of montmorillonite, a smectite mineral known for its high swelling capacity and protein-binding properties. Bentonite is effective for removing heat-unstable proteins from fruit juices.^[25] Activated carbon, produced from natural carbonaceous materials like coconut shells or wood, serves as a clarifying agent by adsorbing colorants and odor compounds in beverages and oils. Polysaccharide-based agents include chitosan, obtained from chitin in crustacean shells or fungal cell walls, which functions through positive charge interactions to neutralize negatively charged particles.^[26] Alginates, extracted from brown seaweed such as Laminaria species, promote clarity via gel formation when cross-linked with divalent cations like calcium.^[27] Enzymatic agents, such as pectinase, are derived from microbial sources and used primarily in fruit juice processing. Pectinase hydrolyzes pectins in fruit cell walls, reducing viscosity and facilitating the release of suspended particles for clarification.^[2] These natural agents offer advantages such as biodegradability, which supports sustainable waste management in food processing, and long-standing acceptance in traditional industries for maintaining product purity.^[28] In contrast to synthetic alternatives, they align with organic certification requirements but may require higher dosages for equivalent efficacy.^[22]

Synthetic Agents

Synthetic clarifying agents are laboratory-produced compounds designed to enhance the removal of suspended particles from liquids through targeted chemical interactions, offering scalability and precision in industrial applications. These agents primarily include inorganic salts, polymeric flocculants, and silicon-based compounds, each engineered for specific coagulation and flocculation roles in processes like water purification and wastewater management. In food and beverage applications, synthetic agents such as polyvinylpyrrolidone (PVPP) are used to remove polyphenols and reduce astringency in wine and beer, while silica-based colloids (e.g., silicon dioxide) target fine protein hazes.^[3] Inorganic salts such as alum, or aluminum potassium sulfate (AlK(SO₄)₂·12H₂O), function by hydrolyzing in water to release positively charged aluminum species that neutralize the negative charges on colloidal particles, promoting their aggregation and sedimentation. This charge neutralization mechanism makes alum a staple in water treatment for clarifying turbid sources by destabilizing fine suspended solids. Polyaluminum chloride (PAC), a pre-hydrolyzed variant with the general formula [Al₂(OH)ₙCl₆₋ₙ]ₘ, improves upon alum by providing higher charge density and faster floc formation, often requiring 30-50% lower dosages while generating less sludge and maintaining broader pH compatibility for efficient turbidity removal in drinking water filtration. Polymeric flocculants, particularly polyacrylamides (PAMs), are high-molecular-weight synthetic polymers classified as anionic, cationic, or non-ionic based on their charge properties, enabling customizable interactions with particles of varying surface charges. These polymers, with molecular weights reaching up to 10⁷ Da, primarily operate via bridging, where extended polymer chains adsorb onto multiple particles to form larger flocs that settle more readily. Cationic PAMs, for instance, are effective for negatively charged inorganic colloids, while anionic variants suit positively charged or organic-laden suspensions, allowing tailored selection for optimal particle destabilization and clarification. Silicon-based agents like sodium silicate (Na₂SiO₃) contribute to coagulation by adjusting pH to alkaline levels, which enhances the solubility and reactivity of metal coagulants while forming silica gels that entrap impurities in wastewater treatment. At dosages of 15-20 mg/L as SiO₂, sodium silicate promotes floc stability and aids in removing silicates and other dissolved solids, particularly in high-pH environments where it acts as both a pH buffer and coagulant aid. Compared to natural clarifying agents, which served as historical precursors derived from plant or animal sources, synthetic agents provide advantages including higher efficiency in particle removal, adjustable charge densities through polymer modification, and reduced dosage requirements that minimize operational costs and residual impacts. A key innovation in this domain is the development of polydiallyldimethylammonium chloride (polyDADMAC) in the 1950s, a cationic polymer that emerged as a non-toxic alternative to metal-based coagulants for potable water treatment, offering effective flocculation without introducing heavy metals.

Mechanisms of Action

Flocculation and Coagulation

Coagulation is the initial process in clarification where suspended particles, typically stabilized by electrostatic repulsion due to their surface charges, are destabilized by the addition of clarifying agents. These agents, such as gelatin, introduce positive charges that neutralize the negative charges on particles like tannins and proteins, reducing the zeta potential—the effective electric potential at the particle-liquid interface—to near zero. This neutralization diminishes the repulsive barrier between particles, allowing attractive van der Waals forces to dominate and promote initial aggregation.^[3] The zeta potential \zeta quantifies this charge stability and is calculated using the Smoluchowski equation under conditions of high ionic strength:

\zeta = \frac{\mu_E \eta}{\varepsilon}

where \mu_E is the electrophoretic mobility, \eta is the viscosity of the medium, and \varepsilon is the dielectric constant. As \zeta approaches zero, colloidal stability decreases, facilitating particle collisions and coalescence.^[29] Flocculation follows coagulation, involving gentle mixing to form larger aggregates known as flocs. In this stage, polymeric clarifying agents like chitosan promote bridging, where long-chain molecules adsorb onto multiple particles, linking them into extended networks that increase floc size and density. Alternatively, agents like bentonite can adsorb proteins and polyphenols directly, contributing to aggregation through surface interactions rather than solely charge precipitation. In juice clarification, enzymatic agents such as pectinase degrade pectins, reducing viscosity and promoting particle coalescence by breaking down stabilizing polysaccharides.^[3]^[2] The underlying particle interactions are described by DLVO theory, which models the total interaction energy as the sum of attractive van der Waals forces and repulsive electrostatic forces. At optimal conditions, the potential energy curve exhibits a secondary minimum, enabling reversible aggregation into flocs without overcoming the primary energy barrier.^[29] Several factors influence the efficacy of coagulation and flocculation. The pH of the solution affects particle charge and agent performance, with optimal ranges for gelatin in wine typically around 3 to 4 to maximize binding with tannins via hydrogen bonding and charge neutralization. Ionic strength modulates the thickness of the electrical double layer around particles, compressing it at higher concentrations to reduce repulsion. Dosage is determined experimentally via bench trials, where gelatin concentrations of 10-150 mg/L are commonly evaluated for wines to achieve clarity without over-fining.^[2]^[30]

Sedimentation and Separation

Sedimentation is a fundamental physical process in the clarification of liquids, where flocculated particles settle under the influence of gravity in dedicated tanks or basins, allowing the denser aggregates to separate from the clearer supernatant liquid. This gravity-driven settling relies on the principles outlined in Stokes' law, which governs the terminal velocity of spherical particles in a viscous fluid. The settling velocity v is given by the equation:

v = \frac{g (\rho_p - \rho_f) d^2}{18 \eta}

where g is the acceleration due to gravity, \rho_p and \rho_f are the densities of the particle and fluid, respectively, d is the particle diameter, and \eta is the fluid viscosity.^[31] This relationship highlights how larger, denser flocs settle more rapidly, enabling efficient separation in industrial settling tanks designed to minimize turbulence and promote laminar flow.^[32] Following sedimentation, filtration methods are employed to capture any remaining fine flocs or suspended solids that do not settle completely. Diatomaceous earth (DE) filtration, a common depth filtration technique, involves pre-coating a filter septum with a porous layer of DE, a siliceous sedimentary rock, which traps particles as the liquid passes through, achieving high clarity without significant pressure loss.^[33] Membrane filters, such as microfiltration or ultrafiltration systems, provide an alternative by using semi-permeable barriers with pore sizes typically ranging from 0.1 to 10 micrometers to retain particulates, often preferred in sterile processing environments for their scalability and ease of validation.^[34] For high-speed separation in viscous liquids, centrifugation applies centrifugal forces to accelerate particle settling; in industrial juice processing, disc-stack centrifuges can generate forces up to 10,000g, rapidly clarifying large volumes by forcing solids to the periphery while the clarified liquid is discharged centrally.^[33] In more delicate applications like winemaking, decantation and racking serve as gentle separation techniques, where the clarified liquid is carefully poured or siphoned off the settled sediment to avoid disturbing the lees at the bottom of the vessel. This process, repeated as needed, not only removes solids but also prevents off-flavors from prolonged contact with deposits.^[35] Overall, these sedimentation and separation methods, often applied sequentially after flocculation, can reduce turbidity from over 100 NTU in raw liquids to less than 1 NTU in the final product, ensuring visual clarity and stability in beverages and treated waters.^[3]

Applications

In Beverages

Clarifying agents play a crucial role in beverage production to achieve visual clarity without compromising sensory qualities, particularly in alcoholic and non-alcoholic drinks where haze can detract from appearance and stability. In winemaking, bentonite is widely employed post-fermentation to prevent protein haze by binding and precipitating heat-unstable proteins in white wines.^[36] Typical addition rates range from 60 to 1,800 mg/L, determined through bench trials to minimize excess that could strip desirable compounds.^[36] Gelatin serves as a fining agent for tannin management in red wines, reducing astringency by forming insoluble complexes with large-molecular-weight tannins at dosages of 30–240 mg/L, followed by 2–3 days of settling.^[37] In brewing, isinglass, derived from fish swim bladders, facilitates yeast removal to enhance beer clarity before packaging. Its positively charged collagen binds to negatively charged yeast cell walls, promoting flocculation and rapid sedimentation in as little as 2 hours when added at 0.25–0.5% of beer volume during transfer.^[38] This process reduces conditioning time to about 3 days while preserving foam stability by eliminating head-negative phospholipids.^[38] For juice and cider production, pectinase enzymes are combined with finings to address fruit-derived hazes caused by pectin. Pectinases break down pectin molecules, enabling fruit particles to flocculate and settle compactly in the lees, often added during pressing with a minimum 2-hour contact time to achieve juice clarity of 80–120 NTU.^[39] Supporting finings such as bentonite or chitosan then accelerate this settling, with bentonite compacting lees at 20–100 g/hL in must, though timing is critical to avoid enzyme inactivation.^[39] A key challenge in beverage clarification is preserving flavor integrity, as over-fining can adsorb volatile compounds essential for aroma. For instance, excessive bentonite application reduces esters like ethyl hexanoate by up to 50% and eliminates terpenes such as linalool, diminishing fruity and floral notes in wines.^[40] Cold stabilization addresses tartrate instability by chilling wine to 0–4°C for 2–3 weeks, inducing potassium bitartrate crystal precipitation to prevent post-bottling formation, though it requires prior clarification to optimize efficacy.^[41] In sparkling wine production via the traditional method, finings like bentonite act as riddling aids added at bottling (up to 6 g/100 L) to compact yeast sediment, facilitating its collection in the bottle neck during remuage and easier removal during disgorging.^[42] Other agents, such as gelatin or isinglass, similarly enhance sediment cohesion for aged wines, reducing riddling time and minimizing wine loss, though overuse may affect foam or palatability.^[42]^[43]

In Water Treatment

In water treatment processes, clarifying agents play a crucial role in municipal and industrial purification by removing suspended solids, turbidity, and contaminants from raw water sources such as rivers. The conventional treatment sequence begins with coagulation, where agents like aluminum sulfate (alum) are dosed into rapid mix tanks to destabilize colloidal particles, followed by flocculation to form larger flocs, and then sedimentation to allow these flocs to settle out.^[44] Typical dosing for alum in river water clarification ranges from 10 to 50 mg/L, depending on water quality parameters like turbidity and alkalinity, to achieve effective particle aggregation without excessive chemical use.^[45] In wastewater applications, clarifying agents such as ferric chloride are employed for targeted phosphorus removal in sewage treatment, where it precipitates phosphates as insoluble ferric phosphate, aiding compliance with effluent discharge limits. This process also generates a sludge that can be dewatered more efficiently compared to untreated solids, reducing disposal volumes in activated sludge systems.^[46] For instance, ferric chloride dosing facilitates the formation of denser flocs that enhance settling and subsequent mechanical dewatering through centrifugation or belt pressing.^[46] Advanced clarification techniques, such as ballasted systems, incorporate microsand as a weighting agent to accelerate floc settling in high-turbidity waters, enabling shorter retention times in clarifiers—often reducing them to 10-20 minutes versus hours in conventional setups. These systems are particularly effective for variable or seasonal high-turbidity inflows, like stormwater-influenced rivers, by recycling the microsand to maintain process efficiency.^[47] Overall, effective use of clarifying agents in these processes routinely achieves effluent turbidity below 0.3 NTU, aligning with WHO guidelines for optimal drinking water quality to ensure pathogen removal and aesthetic clarity.^[48]

In Other Industries

In sugar refining, phosphoric acid and lime are commonly employed as clarifying agents during the processing of sugarcane juice to remove impurities such as waxes, proteins, and fibers. The addition of phosphoric acid reacts with lime (calcium oxide or hydroxide) to form calcium phosphate, which acts as an effective adsorbent, facilitating the flocculation and precipitation of these contaminants for subsequent filtration. This process, often enhanced with polymer flocculants, occurs after juice evaporation and involves aeration to promote separation, ensuring clearer syrup for further refining.^[49]^[50] In the processing of oils and fats, activated clay serves as a key clarifying agent for bleaching vegetable oils by adsorbing pigments such as chlorophyll and carotenoids, which contribute to undesirable coloration. The bleaching process involves mixing the heated oil with acid-activated clay, allowing the porous structure of the clay to selectively bind and remove these pigments along with trace metals and oxidation products, followed by filtration to yield a lighter, more stable oil. Optimization of clay dosage and contact time is critical to balance pigment removal efficiency, typically achieving significant color reduction without excessive oil loss.^[51]^[52] Within the pharmaceutical industry, activated charcoal is utilized for decolorizing syrups and other liquid formulations by adsorbing unwanted pigments and impurities, enhancing product clarity and purity. This adsorption process leverages the high surface area of the charcoal to capture color-causing compounds without altering the active ingredients, commonly applied in the purification of oral syrups during manufacturing. Additionally, synthetic polymers function as flocculants in vaccine purification, aiding the clarification of harvest broths by aggregating cellular debris and host cell proteins for easier separation via centrifugation or filtration, thereby improving yield and downstream processing efficiency.^[53]^[54]^[55] In textile dyeing operations, flocculants are applied to treat effluent containing dyes, promoting coagulation and sedimentation to remove colorants before discharge. These agents, often polymeric, destabilize colloidal dye particles, forming larger flocs that settle out, achieving color removal efficiencies exceeding 70% in real textile wastewater under optimized conditions such as pH adjustment and dosage. This treatment mitigates environmental release of persistent dyes, supporting compliance with effluent standards.^[56]

Safety and Regulations

Health Considerations

Animal-derived clarifying agents pose allergen risks to sensitive individuals; for example, casein can trigger adverse reactions, including anaphylaxis, in those allergic to milk proteins, while gelatin may pose risks for allergies to mammalian proteins such as in alpha-gal syndrome.^[57] In the European Union, mandatory labeling of allergens like milk (including casein) and egg derivatives in foodstuffs has been required since Directive 2003/89/EC, which amended earlier regulations to ensure clear declaration on packaged products, though exemptions applied to certain processing aids in wine until 2012.^[58] In the United States, allergen labeling for fining agents in wine is currently voluntary under Alcohol and Tobacco Tax and Trade Bureau regulations, but in January 2025, the agency proposed mandatory disclosure of major food allergens (including milk, eggs, fish, and others potentially from clarifying agents) on labels for wines and other alcoholic beverages, with a proposed compliance period of five years following finalization.^[59] Toxicity concerns arise from residues of inorganic clarifying agents like alum, which can introduce aluminum into treated water or beverages; the U.S. Environmental Protection Agency sets a secondary maximum contaminant level of 0.05 to 0.2 mg/L for aluminum in drinking water to prevent aesthetic issues and potential health effects from chronic exposure.^[60] Mineral-based clarifying agents, such as bentonite clay, may contain residual heavy metals like lead and arsenic, raising concerns for human exposure through contaminated beverages; the U.S. Food and Drug Administration has issued warnings about elevated lead levels in certain bentonite products, which could lead to neurological damage with prolonged intake.^[61] To address dietary restrictions and allergen avoidance, vegan alternatives like plant-derived proteins (e.g., pea or potato protein) and non-animal substances such as bentonite or silica gel are increasingly promoted, enabling clarification without animal-derived risks.^[62] Post-clarification testing protocols in the beverage industry include sensory evaluations for off-flavors and analytical methods like chromatography and mass spectrometry to detect contaminants or residual agents, ensuring compliance with safety standards and preventing consumer exposure.^[63] A notable case study from 2009-2010 involved a Canadian Food Inspection Agency targeted survey of retail wines for undeclared milk (casein) and egg allergens from fining agents, analyzing over 100 samples; while no detectable levels were found, the initiative highlighted the need for vigilance during the transition to stricter labeling, underscoring potential risks from incomplete removal of allergenic proteins.^[64]

Environmental Impacts

The use of clarifying agents in water treatment processes generates significant sludge as a sedimentation byproduct, particularly from alum-based coagulation, where the resulting sludge typically comprises 1-3% solids by volume relative to the treated water.^[65]^[66] This low-solids material poses disposal challenges, often requiring landfilling or incineration to manage volume and prevent environmental release, contributing to landfill burdens and potential emissions from combustion.^[67]^[68] Non-biodegradable synthetic polymers, such as polyacrylamide used as flocculants in clarification, exhibit high environmental persistence, leading to accumulation in waterways and raising microplastic concerns identified in studies from the 2010s onward.^[69]^[70] These polymers degrade slowly under natural conditions, potentially releasing toxic monomers like acrylamide and forming persistent microplastic fragments that disrupt aquatic ecosystems.^[71] Production of natural clarifying agents like bentonite involves mining that disrupts habitats through land excavation and soil erosion, altering local biodiversity and water flows in extraction areas.^[72] In response, there has been a shift toward bio-based alternatives, such as polymer flocculants derived from starch, cellulose, or chitosan, which offer greater sustainability by reducing reliance on non-renewable resources and minimizing long-term ecological footprints.^[73] Mitigation strategies include recycling fining lees—sediments from clarifying agents in winemaking—into compost, which repurposes organic waste into nutrient-rich soil amendments and reduces disposal needs.^[74] Additionally, the EU's REACH regulation, implemented since 2007, imposes controls on chemical emissions from water treatment substances, promoting safer alternatives and limiting environmental releases of persistent clarifying agents.^[75] A specific concern is eutrophication arising from phosphorus-bound sludges in wastewater plants, where improper disposal of coagulation byproducts can release bound phosphorus into water bodies, fueling algal blooms and oxygen depletion.^[76]^[77]

References

[1]
https://www.ecfr.gov/current/title-21/chapter-I/subchapter-B/part-170/section-170.3
[2]
Effect of clarification on physicochemical properties and nutrient ...
Jan 11, 2023 · A clarifier, also known as fining agent, improves the clarity of the juice by forming an adsorbent, enzymatic, or ionic bond with suspended ...<|control11|><|separator|>
[3]
Combined Effects of Clarifying Agents Improve Physicochemical ...
Jan 17, 2024 · This study utilized gelatin and bentonite as clarifying agents to improve the quality and shelf-life of Indian gooseberry juice (IGBJ).
[4]
[PDF] Procedures for Review of Chemical Additives for Sediment Control
Apr 30, 2019 · Clarifying Agent: chemicals that are used to remove suspended particles from liquids by inducing flocculation. Department: the Maryland ...<|control11|><|separator|>
[5]
Clarification Process - an overview | ScienceDirect Topics
The clarification process is defined as a unit operation that removes suspended and dissolved solids from water, enabling its reuse in various systems.<|control11|><|separator|>
[6]
Clarification of colloidal and suspended material in water using ...
Nov 12, 2015 · Flocculation is a process of bringing together small particles to form larger particles or settling of colloidal particles from stable ...
[7]
Mechanism of clarification of colloidal suspension using composite ...
Flocculation is a process in which individual stable particles dispersed evenly in a suspension are made to aggregate to form flocs capable of settling ...
[8]
21 CFR Part 170 -- Food Additives - eCFR
(24) Processing aids : Substances used as manufacturing aids to enhance the appeal or utility of a food or food component, including clarifying agents, clouding ...Title 21 · 170.20 – 170.39 · 170.203 – 170.285 · 21 CFR 170.3(i)
[9]
When Did Water Filtration Begin? - Clearly Filtered
Plus, written records suggest ancient Egyptians used mined alum (a naturally occurring mineral near the Nile) to coagulate material out of cloudy water. In ...
[10]
History of Drinking Water Treatment - epa nepis
To clarify water, the Egyptians reportedly used the chemi- cal alum as early as 1500 B.C. to cause suspended particles to settle out of water. During the ...
[11]
Tannins? Egg whites? Sturgeon bladders? Here's what's in your wine
Jul 20, 2023 · Winemakers started adding sulfites, olive oil, sea water, and various resins around the founding of ancient Rome (753 BC) to help preserve wine.Missing: clarification 1500
[12]
A brief history of gelatin manufacturing | Rousselot
In the 19th century, gelatin processing and applications transformed into a sizeable industry. Industrial production of gelatin for glue began in Lyon, France, ...
[13]
Gelatin as It Is: History and Modernity - PMC - NIH
At the present time, gelatin is perhaps the most demanded gelling agent in the food industry in general and cooking in particular, and its various types and ...
[14]
[PDF] Coagulation: Ancient and Modern
Some industrial works in Paris used alum to clarify the waters of the Seine as early as the 1820's. Evidence of this is found in two paragraphs in a memorial on ...
[15]
[PDF] Polyacrylamide use for Sediment Reduction in Construction Site ...
Polyacrylamide Uses in Water Treatment and Erosion Control. Polyacrylamides were first used in the 1950s when the nonionic form was used to separate silica ...Missing: invention | Show results with:invention
[16]
Bentonite for wine clarification - Wine History
Aug 10, 2010 · Date or time period: 1934. The use of bentonite in winemaking dates back to Saywell's report and is now universally used for the adsorption ...
[17]
The Safe Drinking Water Act of 1974 and Its Role in Providing ...
This article examines the history, operation, and evolution of the SDWA as a novel vehicle for increasing the citizenry's access to safe drinking water.
[18]
Coagulant Recovery from Water Treatment Residuals - NIH
Recovered Coagulant Quality. In the 1970s and 1980s, when water quality regulations were less focused on DBPs and metals, nonselective CR was capable of ...
[19]
Wine Fining with Plant Proteins - PMC - NIH
Jun 11, 2019 · As a consequence nowadays porcine gelatin is probably the most commonly used protein for wine fining, as it preferentially removes high ...1. Introduction · 2. Wine Fining With Plant... · 2.1. 1. Gluten
[20]
Allergenic Proteins in Enology: A Review on Technological ... - NIH
Isinglass is a positively charged fining agent derived from the air bladder of certain fish species such as sturgeon. Isinglass is used in still, dry, sweet and ...<|separator|>
[21]
[PDF] Where is allergen labelling at?
If the wine contains any significant amount of residual protein from the use of egg (ovalbumin), fish (isinglass) or milk (casein) products as a fining agent, ...
[22]
[PDF] Bentonite Fining of Juice and Wine
Gelatin can be used to help flocculate bentonite and possibly aid in lees compaction. A discussion of the use of protein fining agents is given by Zoecklein ...
[23]
[PDF] Chitosan - Semantic Scholar
- Clarifying agent: due to its cationic nature, chitosan can interact with anions through electrostatic interactions, as well as via hydrogen bonds and van ...
[24]
Sodium Alginate - KIMICA Corporation
Sodium alginate is a neutral salt, soluble in water, that forms a gel with calcium ions. It is used as a thickener, gelling agent, and stabilizer.
[25]
The Use Of Chitosan As Clarification Agent - ResearchGate
Oct 17, 2016 · Being nontoxic, biodegradable and as a coagulation agent ... The use of edible coatings in food industry, packaging offers many advantages.<|separator|>
[26]
A review of coagulation explaining its definition, mechanism ...
This review will discuss the coagulation process, coagulant types and aids in addition to the factors affecting the coagulation process.Missing: customizable | Show results with:customizable
[27]
Flocculant and Coagulant Water Treatment | ChemTreat, Inc.
Flocculation is when smaller particles form larger masses called flocs. Flocculants gather destabilized particles to cause them to agglomerate and drop out of ...
[28]
An overview of surface forces and the DLVO theory | ChemTexts
Jul 22, 2023 · Flocculation in a colloidal dispersion can be observed only if the secondary minimum is deep enough to prevent both re-dispersion and ...
[29]
Prediction of the optimal dosage of coagulants in water treatment ...
Jar test operation was performed applying doses 5, 10, 15, 20, 25, 30 mg/L of aluminum sulfate (Alum) and poly-aluminum chloride (PAC) coagulants, separately.
[30]
Sedimentation-Based Separation and Purification of Solid Industrial ...
Nov 13, 2023 · The sedimentation process is expounded upon using both the classical mechanics model and Stokes' law. To foster a seamless industrial ...Missing: clarifying | Show results with:clarifying
[31]
Noninvasive Measurement of Particle-Settling Velocity and ...
Aug 7, 2025 · While the smaller particles settled at a rate closely predicted by Stokes' law, the larger particles settled slightly more slowly than was ...
[32]
[PDF] Diatomaceous Earth Filtration for Drinking Water - wvdhhr.org
DE filtration relies upon a layer of diatomaceous earth placed on a filter element or septum and is frequently referred to as pre-coat filtration.
[33]
Depth Filters Containing Diatomite Achieve More Efficient Particle ...
Centrifuges can be used for clarification but single-use filters are preferred because these are less expensive, more scalable and do not require cleaning ...
[34]
https://pmc.ncbi.nlm.nih.gov/articles/PMC4685141/
[35]
[PDF] Decanting - racking) - OIV
Objectives : a) To separate wines from their lees, deposited on the bottom of the container. b) To prepare for mixing or blending operations.
[36]
Water Handbook - Clarification | Veolia
In-line clarification is generally limited to raw waters with typical turbidities of less than 20 NTU, although upflow filters may tolerate higher loading.
[37]
[PDF] Fining with Bentonite - Purdue Extension
Bentonite clay is the most widely used fining agent against heat-instable grape proteins in white wines. In red wines from Vitis vinifera, the inherent tannins.
[38]
[PDF] A Guide to the Fining of Wine - Sandiego
Gluten, a wheat protein, has been effective as a red and white wine fining and clarification agent (Maury et al. 2003, Marchal et al. 2002). Although not widely.
[39]
[PDF] Clear Beer Through Finings Technology - RahrBSG
Positively charged isinglass is attracted to the yeast cell walls which possess a negative charge and adheres the cells together, thereby increasing the floc ...
[40]
Cider Clarification 101 - Scott Laboratories
Enzymes, specifically pectinases, break down pectin, allowing fruit particles to settle and compact in the lees. Step 2: Add Fining Agents. Fining agents such ...
[41]
Effect of Different Clarification Treatments on the Volatile ... - NIH
Jun 8, 2020 · The aim of this study was to evaluate the influence of clarification treatments on volatile composition and aromatic attributes of wine samples.
[42]
[PDF] Methods for Tartrate Stabilization of Wine
Other factors such as the concentration of acids, cations, anions,. pH, and various complexing agents will also affect the tartrate stability and precipitation.
[43]
[PDF] A REVIEW OF MÉTHODE CHAMPENOISE PRODUCTION
Section 4. Riddling Aids. To enhance riddling ability, disgorgement, and possibly wine palatability, some vintners add riddling aids at the time of cuvée ...
[44]
[PDF] Sparkling Handbook - Scott Laboratories
Tartrate stabilization can be achieved by cold stabilization or by the use of inhibitory products such as carboxymethylcellulose (CMC), which inhibit crystal ...<|separator|>
[45]
How Water Treatment Works | Drinking Water - CDC
Feb 6, 2024 · Water utilities often use a series of water treatment steps that include coagulation, flocculation, sedimentation, filtration, and disinfection.
[46]
[PDF] Water Treatment Manuals COAGULATION, FLOCCULATION ...
A dose of 1 mg/l of aluminium sulphate reacts with 5.3 mg/l of alkalinity expressed as CaCO3 . Thus if no alkali is added the alkalinity will be reduced by this ...
[47]
[PDF] Phosphorus Treatment and Removal Technologies
Phosphorus removal is most efficient in the pH range of 5 to 7 for alum and of 6.5 to 7.5 for ferric salts since their precipitates will not readily return to ...
[48]
Actiflo® | Veolia Water Technologies
Actiflo is a high-rate, compact water clarification system for drinking water, using ballasted flocculation, and is effective for removing turbidity and algae.
[49]
Water quality and health: Review of turbidity
Feb 14, 2017 · This technical brief provides information on the uses and significance of turbidity in source water and drinking-water.
[50]
Improved Sugar Cane Juice Clarification by Understanding Calcium ...
Aug 6, 2025 · Phosphates react with lime and form calcium phosphate, which is a good adsorbent and helps remove impurities, such as waxes, proteins, fibre, ...
[51]
[PDF] AP-42, Section 9.10.1.1: Sugarcane Processing
Following evaporation, the syrup is clarified by adding lime, phosphoric acid, and a polymer flocculent, aerated, and filtered in the clarifier.
[52]
Optimization of Bleaching Process - AOCS
Originally described as a process of mixing oil and clay adsorbent to remove color, the bleaching operation effectively removes some of the color, reduces the ...
[53]
Effect of bleaching with different clay on the final composition of post ...
Jul 15, 2023 · Bleaching clays are excellent at removing the pigments present in vegetable oils ... Maximum bleaching of vegetable oils by acid-activated ...
[54]
Decolorization with Activated Carbon in the Pharmaceutical Industry
Sep 4, 2023 · Activated carbon is used to remove impurities, like unwanted pigments and dyes, by adsorption, improving visual appearance and product quality.Missing: syrups | Show results with:syrups
[55]
Practical and Sustainable Modification Method on Activated Carbon ...
Feb 15, 2021 · The decolorization section is an important part of APAP pharmaceutical engineering; the powdered activated carbon (PAC) is a commonly used ...Missing: syrups | Show results with:syrups
[56]
[PDF] Clarification of vaccines - DCVMN
Clarification of vaccines is a critical step that impacts product recovery and purification, using techniques like membrane filtration, centrifugation, and ...
[57]
Real textile effluents treatment using coagulation/flocculation ...
Coagulation/flocculation can remove color from real textile effluent with >70% of efficiency. •. L. sativa and R. subcapitata can be used for ecotoxicological ...
[58]
Tips on Managing Sugar Sand | Ohio State Maple
Mar 25, 2015 · The key to removing niter. You must keep it in the solution until you run it through your filter, and you need to keep your syrup very hot and filter it ...
[59]
Risk of allergic reactions to wine, in milk, egg and fish-allergic patients
Oct 17, 2011 · Casein, isinglass or egg, remaining in traces in wine after fining, present a very low risk for the respective food allergic consumers.Missing: recalls undeclared 2010
[60]
European Regulations for Labeling Requirements for Food ...
Aug 10, 2025 · The present paper describes the development of European directives and regulations for the labeling of food allergens and intolerances to substances like ...
[61]
Secondary Drinking Water Standards: Guidance for Nuisance ...
Jun 2, 2025 · Table of Secondary Standards ; Aluminum, 0.05 to 0.2 mg/L *, colored water ; Chloride, 250 mg/L · salty taste ; Color, 15 color units, visible tint.
[62]
FDA Warns Consumers Not to Use “Best Bentonite Clay”
Mar 23, 2016 · FDA laboratories have found elevated levels of lead in “Best Bentonite Clay.” Exposure to lead can cause serious damage to the central nervous ...
[63]
Plant-Based Fining Agents: Free of Animal Proteins - Enartis
You can say goodbye to animal protein for clarification and make high quality wines with entirely plant-based and allergen-free products.Missing: dietary restrictions
[64]
Foreign Material and Off-Odor and Flavor Testing - Eurofins USA
Mar 1, 2024 · By utilizing personalized testing plan, you can stop product contamination and identify the source or location of contamination from the start.
[65]
2009-2010 Fining Agents in Wine - inspection.canada.ca
Sep 4, 2018 · Samples of the wine were analysed for milk (casein and beta-lactoglobulin) and egg allergens using a proven method validated in multiple ...
[66]
A review on alum sludge reuse with special reference to agricultural ...
The key perennial issue associated with alum sludge is its cost effective and efficient disposal due to the large sludge volumes and environmental drawbacks ...Missing: landfilling incineration
[67]
Coagulant Solids
The resulting aluminum hydroxide species, or coagulant sludge, is such that 1 mg/L of alum as dry weight product added to water will produce approximately 0.44 ...
[68]
Sludge Disposal Methods in Wastewater: Efficient and Sustainable ...
Acceptable methods for sludge disposal include landfilling, incineration, and application to agricultural land. Each method must comply with regulations ...
[69]
[PDF] SLUDGE CHARACTERISTICS AND DISPOSAL ALTERNATIVES ...
Incineration, in its various forms, is the most common alterna- tive disposal method (Table V). It decreases the volume of ma- terial that needs to be ...
[70]
[PDF] Ecotoxicological effects of polyacrylate, acrylic acid, polyacrylamide ...
The first part of this report focused on the properties, environmental behaviour and environmental release of linear PAMs and cross-linked PAMs/PAAs. Both ...Missing: microplastics | Show results with:microplastics
[71]
Are We Speaking the Same Language? Recommendations for a ...
Jan 4, 2019 · For instance, polyacrylamide copolymers are used as flocculation agents ... Sources, fate and effects of microplastics in the marine environment ...<|separator|>
[72]
Environmental Degradation of Polyacrylamides - ScienceDirect.com
The results of this study suggest that polyacrylamide can degrade to acrylamide under environmental conditions. Statistically, there was no linear correlation ...Missing: persistence | Show results with:persistence
[73]
[PDF] WYW-165988 - BLM National NEPA Register
7) Disturbance caused by the proposed bentonite mining would affect suitable habitat for migratory birds. Mountain plover occur in the area and surveys ...
[74]
Recent Achievements in Polymer Bio-Based Flocculants for Water ...
Strictly speaking, the flocculation efficiency is higher if the settling time is shorter and the flocculant dose is lower. This last term is also called optimum ...Missing: customizable | Show results with:customizable
[75]
Composting Waste from the White Wine Industry - MDPI
Stalks and sludge may be recycled and valorized through the composting process [26]. Grape marc and lees are considered products with value, and wineries can ...
[76]
REACH Regulation - Environment - European Commission
The REACH Regulation (EC 1907/2006) entered into force in 2007 and has since evolved to reflect the advancement of knowledge regarding various chemicals and ...Missing: clarifying | Show results with:clarifying
[77]
Use of wastewater alum-coagulation sludge as a phosphorus fertiliser
Feb 17, 2024 · Phosphorus (P) discharged into natural water bodies, such as lakes and rivers, through wastewater can trigger nutrient enrichment, fostering ...
[78]
Nutrient removal and sludge production in the coagulation ...
Nutrients present may be removed during the process. The presence of phosphorus in the wastewater should be limited since it can cause eutrophication of surface ...