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DLG4

DLG4 is a human gene located on the short arm of chromosome 17 (17p13.1) that encodes postsynaptic density protein 95 (PSD-95), also known as synapse-associated protein 90 (SAP-90), a member of the membrane-associated guanylate kinase (MAGUK) family of scaffolding proteins essential for synaptic organization.^[1]^[2] The PSD-95 protein is predominantly expressed in the brain, where it localizes to the postsynaptic density of excitatory synapses, forming multimeric scaffolds that cluster ionotropic receptors such as NMDA and AMPA receptors, potassium channels, and associated signaling molecules to regulate synaptic transmission and plasticity.^[2]^[3] This scaffolding function is critical for synaptogenesis, neuronal signaling, and processes underlying learning and memory, with PSD-95 heteromultimerizing with other MAGUK proteins like DLG2 to enhance synaptic stability.^[2]^[1] Pathogenic variants in DLG4, typically de novo heterozygous loss-of-function mutations such as frameshifts or nonsense variants, lead to DLG4-related synaptopathy (also known as SHINE syndrome), an autosomal dominant neurodevelopmental disorder.^[4]^[5] This condition is characterized by intellectual disability (present in 100% of reported cases, often mild to moderate), global developmental delay (84%), autism spectrum disorder (56%), epilepsy or seizures (53%), hypotonia (53%), and movement disorders (46%), with additional features including sleep disturbances, strabismus, joint hypermobility, and scoliosis.^[4] The disorder arises from impaired synaptic function and plasticity due to haploinsufficiency of PSD-95, disrupting neuronal connectivity and brain development.^[4]^[2] Diagnosis is confirmed through molecular genetic testing identifying DLG4 variants, and management focuses on supportive therapies for neurodevelopmental and behavioral symptoms.^[4]

Gene

Location and organization

The DLG4 gene, officially symbolized as DLG4 by the HUGO Gene Nomenclature Committee, encodes the discs large homolog 4 protein, also known as postsynaptic density protein 95 (PSD-95) or synapse-associated protein 90 (SAP90). Its UniProt accession number is P78352 for the human isoform.^[6] In humans, DLG4 is located on the short arm of chromosome 17 at band 17p13.1, spanning genomic coordinates 7,187,187 to 7,219,836 (GRCh38.p14 assembly, reverse strand).^[2] In mice, the orthologous Dlg4 gene resides on chromosome 11 at coordinates 69,908,029 to 69,938,107 (GRCm39 assembly).^[7] The human DLG4 gene spans approximately 30 kb and consists of 22 exons, with exon sizes ranging from 28 to 1,218 nucleotides; all intron-exon junctions follow the GT-AG rule.^[8] Alternative splicing of DLG4 produces multiple transcripts, including the primary isoforms PSD-95α (lacking an N-terminal insertion) and PSD-95β (containing a 24-amino-acid N-terminal extension encoded by exon 1β).^[6] These isoforms arise from the use of alternative first exons, contributing to tissue-specific expression variations.^[8] DLG4 exhibits strong evolutionary conservation, particularly in mammals, with the encoded PSD-95 protein sharing 99% sequence identity between human, mouse, and rat orthologs. Orthologs are also present in invertebrates, such as the Drosophila melanogaster discs large 1 (dlg1) gene, which shares functional and structural similarities as a founding member of the MAGUK family. This conservation underscores DLG4's essential role in synaptic organization across species.^[2]

Expression patterns

The DLG4 gene, encoding the postsynaptic density protein PSD-95, exhibits highly specific expression patterns predominantly within the central nervous system. It is markedly enriched in brain neurons, with mRNA levels showing strong specificity to neural tissues compared to other organs.^[9] Within the brain, DLG4 expression is particularly prominent in the postsynaptic densities of excitatory synapses, serving as a key scaffold in regions such as the hippocampus and cerebral cortex, where it supports synaptic organization and plasticity.^[10]^[11] This neuronal enrichment underscores its role in excitatory neurotransmission, with transcript levels detected across both gray and white matter in adult brains.^[12] Developmentally, DLG4 expression follows a tightly regulated timeline aligned with synaptogenesis. During embryonic stages, such as embryonic day 18 (E18) in rodents, mRNA levels remain low, reflecting minimal synaptic maturation at this point. Postnatally, expression rises progressively, with significant upregulation observed from postnatal day 7 (P7) through P21 in rodent brains, coinciding with peak periods of synapse formation and strengthening in the hippocampus and cortex.^[13] This temporal pattern ensures that DLG4 is available to facilitate the assembly of postsynaptic complexes as neural circuits mature. Although primarily neuronal, DLG4 displays low-level expression in non-neuronal tissues, including the heart, lung, and testis, as evidenced by transcriptome data from human samples.^[14]^[9] Emerging studies also indicate detectable expression in non-neural cells, such as epithelial tissues, where an alternatively spliced form of DLG4 may contribute to cellular polarization and organization.^[15] DLG4 expression is governed by specific promoters and enhancers that respond to developmental and environmental cues. In neurons, it is influenced by activity-dependent mechanisms, including signaling through CREB transcription factors, which integrate synaptic inputs to modulate transcript levels during plasticity and circuit refinement.^[16]^[17]

Protein

Primary structure

The DLG4 gene encodes the postsynaptic density protein 95 (PSD-95), a member of the membrane-associated guanylate kinase (MAGUK) family. The canonical isoform, PSD-95α, consists of 724 amino acids with a calculated molecular weight of approximately 80.5 kDa, though the observed molecular weight is around 95 kDa due to post-translational modifications.^[18]^[6] The protein sequence features two N-terminal cysteine residues (Cys3 and Cys5) in PSD-95α that are subject to palmitoylation, facilitating membrane association.00793-7) Two major isoforms of PSD-95 are produced through alternative splicing of the N-terminal region: PSD-95α and PSD-95β. PSD-95α includes a short N-terminal sequence with the palmitoylation motif, comprising the full 724 amino acids and representing the predominant form in the brain (about 80-90% of expression). In contrast, PSD-95β features an alternative N-terminal extension of 53 amino acids containing an L27 domain for protein-protein interactions, lacking the palmitoylation cysteines, and results in a slightly shorter protein of approximately 711 amino acids. This isoform-specific splicing occurs via mutually exclusive use of exons encoding the N-terminal regions, with PSD-95β generated by inclusion of β-specific exons (β1, β1', β1"). A truncated isoform also exists but is less abundant.^[19]^[20]^[21] Post-translational modifications of PSD-95 include palmitoylation, phosphorylation, and ubiquitination. In PSD-95α, palmitoylation occurs dynamically at Cys3 and Cys5, enabling synaptic targeting and cycling. Phosphorylation targets multiple serine and threonine residues, such as Ser295 (S295) in the linker region between PDZ domains, mediated by calcium/calmodulin-dependent protein kinase II (CaMKII), along with other sites like Ser73 (S73) and Thr19 (T19). Ubiquitination occurs on lysine residues, primarily regulated by E3 ligases like Mdm2, marking the protein for proteasomal degradation without specified dominant sites but affecting overall stability.00793-7)^[22]^[23] As a MAGUK family member, PSD-95 exhibits sequence homology to other proteins like PSD-93 (DLG2) and SAP97 (DLG1), particularly in conserved regions encompassing the three PDZ domains (approximately 80-90 residues each), the SH3 domain, and the guanylate kinase (GK)-like domain, which together comprise about 60% of the protein length and share 70-90% identity across MAGUKs.^[19]^[6]

Domains and motifs

The PSD-95 protein, encoded by the DLG4 gene, belongs to the membrane-associated guanylate kinase (MAGUK) superfamily of scaffolding proteins, which are characterized by a conserved PDZ-SH3-GK modular cassette that facilitates the organization of multiprotein complexes at cellular membranes.^[24] This architecture enables PSD-95 to act as a core organizer in postsynaptic densities, linking transmembrane receptors to intracellular signaling and cytoskeletal elements.^[25] PSD-95 contains three tandem N-terminal PDZ domains (PDZ1, PDZ2, and PDZ3), each approximately 80-90 amino acids long, which recognize and bind short C-terminal peptide motifs (typically -X-S/T-X-Φ, where Φ is a hydrophobic residue) on target proteins.^[25] PDZ1 and PDZ2 preferentially interact with the C-terminal motifs of auxiliary subunits like stargazin, which traffics AMPA receptors to synapses, while PDZ3 binds the -ESDV motif at the C-terminus of neuroligin-1, promoting trans-synaptic adhesion.^[26]^[27] The central SH3 domain of PSD-95, comprising about 70 residues with a typical β-barrel fold, is positioned between PDZ3 and the GK domain and binds proline-rich sequences (P-X-X-P motifs) in partner proteins such as the SAPAP family (also known as GKAPs or DLGAPs).^[25] This interaction, though secondary to GK-mediated binding in some cases, contributes to the recruitment of cytoskeletal regulators.^[28] The C-terminal guanylate kinase (GK) domain, homologous to yeast guanylate kinase but catalytically inactive due to key substitutions (e.g., in the ATP-binding site), adopts a bilobal structure and primarily functions in protein-protein interactions rather than nucleotide metabolism.^[25] It binds the C-terminal regions of GKAPs/SAPAPs with micromolar affinity (e.g., K_d ≈ 1-2 μM) and mediates homodimerization of PSD-95 molecules through a conserved interface, stabilizing synaptic scaffolds.^[29]^[30] In addition to the core MAGUK cassette, PSD-95 features an N-terminal L27 domain in its β isoform, a ~120-residue module that promotes multimerization and heterodimerization with other L27-containing proteins like SAP97, enhancing synaptic clustering.^[21] The protein's extreme C-terminus includes a PDZ-binding motif that enables intermolecular oligomerization by engaging the PDZ domains of adjacent PSD-95 molecules, further amplifying scaffold assembly.^[31]

Function

Synaptic scaffolding

PSD-95, the protein encoded by the DLG4 gene, serves as a primary scaffolding molecule in the postsynaptic density (PSD) of excitatory synapses, where it organizes and stabilizes a multitude of synaptic components essential for neuronal communication. Localized to the PSD through N-terminal palmitoylation, PSD-95 forms clusters that anchor ionotropic glutamate receptors, thereby maintaining the structural integrity of the postsynaptic apparatus.^[32] This localization enables PSD-95 to directly and indirectly cluster N-methyl-D-aspartate (NMDA) receptors and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors at synaptic sites, facilitating their proper positioning and synaptic efficacy.^[33]^[34] During synapse maturation, PSD-95 plays a critical role in promoting dendritic spine formation and stabilization, particularly in developing neurons. By organizing the PSD scaffold, it supports the transition from immature filopodia to mature mushroom-shaped spines, which is vital for excitatory synapse assembly and overall circuit refinement.^[35] PSD-95 also regulates receptor trafficking by directing the insertion of glutamate receptors into the plasma membrane and modulating their endocytosis through specific motifs, ensuring dynamic control over synaptic strength without delving into downstream signaling. These functions rely on PSD-95's modular domains, such as PDZ motifs, which facilitate its anchoring capabilities (detailed in the Protein section). Evidence from animal models underscores PSD-95's indispensable role in synaptic organization. In PSD-95 knockout mice, dendritic spine density is reduced by 15% in the striatum but increased by approximately 40% in the hippocampus.^[36] Additionally, these mutants exhibit altered long-term potentiation (LTP), with enhanced LTP induction that impairs learning.^[37] Knockdown studies further confirm that PSD-95 deficiency arrests synapse development, reducing functional synapse numbers and PSD size.^[35]

Signal transduction

PSD-95, encoded by the DLG4 gene, plays a central role in coupling NMDA receptor (NMDAR) activation to downstream Ras/ERK signaling pathways through its PDZ domains. By binding the C-terminal tails of NMDAR subunits (primarily NR2A and NR2B) via PDZ1 and PDZ2, PSD-95 scaffolds the receptor in proximity to regulatory proteins like SynGAP, a Ras GTPase-activating protein (GAP) that associates with PSD-95's C-terminal region.^[38] Upon NMDAR activation and calcium influx, this complex modulates Ras activity; SynGAP negatively regulates Ras-ERK signaling to fine-tune synaptic plasticity, preventing excessive activation that could disrupt balanced neurotransmission.^[38] Disruption of this PDZ-mediated linkage impairs ERK phosphorylation and alters synaptic strengthening.^[38] In AMPA receptor modulation, PSD-95 facilitates the phosphorylation of the GluA1 subunit and subsequent synaptic strengthening during long-term potentiation (LTP). PSD-95 interacts with transmembrane AMPA receptor regulatory proteins (TARPs), such as stargazin, which bind the GluA1 C-terminus and link it to PSD-95's PDZ domains, stabilizing AMPA receptors at the synapse. This scaffolding enables kinases like CaMKII to phosphorylate GluA1 at Ser831, enhancing channel conductance and promoting AMPA receptor insertion into the postsynaptic membrane, a key step in LTP induction. Such interactions ensure activity-dependent trafficking and potentiation of AMPA-mediated currents, supporting synaptic efficacy. PSD-95 also orchestrates calcium signaling by scaffolding ion channels and kinases, notably linking NMDARs to neuronal nitric oxide synthase (nNOS) via its PDZ2 domain. The C-terminal PDZ-binding motif of nNOS binds PSD-95's PDZ2, positioning nNOS near calcium-permeable NMDARs to sense influx upon activation. This proximity activates nNOS to produce nitric oxide (NO), which diffuses to activate soluble guanylate cyclase in adjacent cells, elevating cyclic GMP (cGMP) levels and modulating downstream targets like protein kinases for synaptic modulation. This pathway supports local calcium-dependent signaling without requiring direct enzymatic coupling. PSD-95 is critical for hippocampal-dependent learning and memory, particularly spatial tasks, as evidenced by deficits in DLG4 knockout models. Mice lacking PSD-95 exhibit impaired performance in spatial learning paradigms, such as the Morris water maze, despite enhanced LTP, indicating a regulatory role in translating synaptic plasticity into behavioral outcomes.^[37] These disruptions highlight PSD-95's necessity for balancing excitability and memory consolidation in the hippocampus.

Pathophysiology

Disease associations

DLG4-related synaptopathy, also known as SHINE syndrome, is a rare neurodevelopmental disorder primarily caused by heterozygous loss-of-function variants in the DLG4 gene, leading to impaired synaptic scaffolding essential for neuronal communication.^[4] The condition was first described in 2021 through the identification of pathogenic variants in cohorts screened for intellectual disability, autism spectrum disorder, and related neurodevelopmental issues.^[10] Core clinical features include global developmental delay (affecting approximately 84% of cases), intellectual disability (in 100% of reported individuals, typically mild to moderate), and autism spectrum disorder traits (observed in 56%).^[10]^[4] Language delays are prominent, often manifesting as delayed speech acquisition and expressive language impairments, alongside social communication challenges.^[4] Epilepsy is a significant comorbidity, occurring in about 50-53% of affected individuals, with onset typically around age 6 years and encompassing both generalized and focal seizure types.^[4]^[10] A notable subset involves developmental epileptic encephalopathy (DEE), particularly electrical status epilepticus in sleep (ESES) or DEE with spike-wave activation during sleep (DEE-SWAS), affecting more than 25% of those with epilepsy and contributing to further neurodevelopmental regression.^[39] These epileptic phenotypes often correlate with sleep disturbances (in 45% of cases) and may exacerbate cognitive and behavioral challenges.^[4] Additional phenotypes include hypotonia (53%), motor delays (such as gait abnormalities and coordination issues in 46%), and behavioral concerns like attention-deficit/hyperactivity disorder (57%) and anxiety (53%).^[4]^[10] Less common features encompass joint laxity (37%), scoliosis (20%), strabismus, and vomiting (29%), with some individuals displaying a Marfanoid habitus.^[4] Intellectual regression, involving loss of previously acquired motor or language skills, occurs in approximately one-third of cases, as highlighted by a 2024 report of a novel de novo frameshift variant (c.554_563del) in a child with onset at age 2.^[40] The disorder is ultra-rare, with fewer than 130 reported cases worldwide as of 2024, though exact prevalence remains unknown due to underdiagnosis.^[41] Variants are predominantly de novo and heterozygous, with loss-of-function mechanisms accounting for the majority, supporting an autosomal dominant inheritance pattern.^[4]

Pathogenic mechanisms

Pathogenic variants in DLG4 primarily lead to loss-of-function effects through haploinsufficiency, resulting in reduced PSD-95 protein levels that impair synaptic stability and the clustering of postsynaptic receptors such as AMPA and NMDA types.^[4] This reduction disrupts the scaffolding role of PSD-95 at excitatory synapses, leading to diminished synapse maturation and maintenance, as evidenced by decreased synaptic density and altered excitatory transmission in cellular models.^[42] Reported DLG4 variants include frameshift and nonsense mutations that trigger nonsense-mediated decay, such as the de novo heterozygous nonsense variant c.1840C>T (p.Arg614*), which was classified as likely pathogenic and associated with intellectual disability.^[43] Missense variants often affect critical PDZ domains, disrupting protein-protein interactions essential for synaptic organization, while frameshifts like c.554_563del (p.Gly185AlafsTer4) further contribute to protein truncation and functional loss.^[4]^[40] These variant types collectively support a dominant-negative or haploinsufficient mechanism, with most occurring de novo.^[10] At the synaptic level, DLG4 disruptions cause deficits including reduced AMPA and NMDA receptor-mediated currents, abnormal dendritic spine morphology with fewer mature spines, and impaired synaptic plasticity, as observed in Dlg4 knockout mouse models and inferred from human variant studies.^[44] Patient-derived neurons harboring DLG4 variants exhibit similar impairments, with decreased excitatory postsynaptic currents and disrupted long-term potentiation, highlighting a core synaptopathy.^[42] Recent studies from 2023 to 2025 have linked DLG4 variants to developmental epileptic encephalopathy (DEE), particularly encephalopathy with status epilepticus during slow-wave sleep (ESES), where abnormal slow-wave sleep oscillations correlate with cognitive regression due to disrupted synaptic homeostasis.^[39] In animal models of Dlg4 deficiency, cognitive and motor deficits, such as impaired learning and coordination, are reversible upon restoration of PSD-95 expression via epigenetic editing or artificial transcription factors, underscoring the potential for targeted therapeutic interventions.^[45]

Interactions

Direct binding partners

PSD-95, encoded by the DLG4 gene, interacts directly with several key synaptic proteins through its modular domains, primarily the PDZ domains, which recognize specific C-terminal motifs in binding partners.^[46] Members of the neuroligin family, such as neuroligin-1, bind to the third PDZ domain (PDZ3) of PSD-95 via their C-terminal -HV motif, a class I PDZ-binding sequence that facilitates postsynaptic adhesion and synapse specificity. This interaction is crucial for recruiting PSD-95 to nascent synapses during excitatory synaptogenesis.^[47] For glutamate receptors, the NR2B subunit of NMDA receptors binds directly to the second PDZ domain (PDZ2) of PSD-95 through its C-terminal ES DV motif, enabling receptor clustering at the postsynaptic density.^[46] In contrast, AMPA receptors do not bind PSD-95 directly but associate indirectly via the auxiliary subunit stargazin, whose C-terminal TVI motif interacts with PDZ3 of PSD-95 to stabilize AMPA receptor localization at synapses.^[26] Shaker-type potassium channels, including Kv1.4, bind to the PDZ domains of PSD-95 (primarily PDZ1 and PDZ2) via their C-terminal tSXV motif, promoting channel clustering in the postsynaptic membrane.^[48] Among scaffolding proteins, the SAPAP family (also known as guanylate kinase-associated proteins or GKAPs) interacts with PSD-95 through multiple interfaces: SAPAPs bind the SH3 domain via proline-rich regions, while GKAP specifically engages the guanylate kinase (GK) domain, linking PSD-95 to cytoskeletal elements like Shank.^[49] These bindings, which leverage the modular structure of PSD-95 described in the protein section, support the assembly of postsynaptic scaffolds.

Regulatory complexes

PSD-95 forms a ternary complex with N-methyl-D-aspartate receptors (NMDARs) and neuronal nitric oxide synthase (nNOS), which couples glutamate-mediated synaptic activation to nitric oxide (NO) production.^[50] In this complex, PSD-95's PDZ domains bind the C-terminal tails of NMDAR subunits GluN2A and GluN2B, while also interacting with the PDZ domain of nNOS, thereby tethering nNOS to the postsynaptic density (PSD) for efficient activation upon calcium influx through NMDARs.^[51] This linkage facilitates NO synthesis, which acts as a retrograde messenger to modulate presynaptic release and contribute to synaptic plasticity, such as long-term potentiation (LTP), by influencing cyclic GMP signaling in target neurons.^[52] Additionally, nNOS-derived NO from this complex promotes vasodilation by diffusing to adjacent vascular smooth muscle cells, thereby regulating cerebral blood flow in response to neuronal activity.^[53] Another key regulatory complex involves PSD-95 and the SAP90/PSD-95-associated proteins (SAPAPs, also known as DLGAPs or GKAPs), which bridge the PSD to the actin cytoskeleton for dendritic spine stabilization. SAPAPs bind directly to the guanylate kinase-like (GK) domain of PSD-95, while their C-terminal regions interact with Shank proteins, which in turn recruit actin-binding partners like cortactin and Homer to anchor the cytoskeleton. This PSD-95/SAPAP/Shank scaffold maintains spine morphology and integrity by linking ionotropic glutamate receptors to F-actin dynamics, preventing spine shrinkage or loss under basal conditions and supporting structural plasticity.^[54] Disruptions in this complex, such as SAPAP mutations, lead to altered spine density and synaptic scaling, underscoring its role in organizing the postsynaptic architecture. The neuroligin-PSD-95 system coordinates with the β-catenin-containing adherens junction complex to mediate trans-synaptic adhesion and modulate Wnt signaling at excitatory synapses. Neuroligins, postsynaptic adhesion molecules, bind extracellularly to presynaptic neurexins to initiate synapse formation, while their intracellular tails interact with PSD-95's PDZ domains to recruit scaffolding elements.^[55] β-Catenin associates with this assembly via interactions with cadherins, stabilizing adherens junctions and facilitating trans-synaptic bridging that aligns pre- and postsynaptic specializations. This coordination also integrates Wnt/β-catenin signaling, where stabilized β-catenin translocates to influence gene expression for synaptic proteins, thereby fine-tuning adhesion strength and synapse maturation.^[55] These regulatory complexes exhibit dynamic assembly and disassembly in response to synaptic activity, particularly during LTP, mediated by calcium/calmodulin-dependent protein kinase II (CaMKII) phosphorylation of PSD-95. Upon LTP induction, CaMKII phosphorylates PSD-95 at serine 73 within its N-terminal domain, promoting transient PSD destabilization that allows receptor trafficking and spine enlargement before restabilization.^[56] This phosphorylation-dependent remodeling enables activity-induced reconfiguration of complex components, such as enhanced recruitment of NMDARs to the PSD-95/nNOS module or SAPAP-mediated cytoskeletal adjustments, ensuring adaptive synaptic strengthening without chronic disruption.^[57]

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[46]
PSD-95 Assembles a Ternary Complex with theN-Methyl-d-aspartic ...
To prevent both PDZ1 and PDZ2 from being bound by the NR2B fusion protein in vitro, we first incubated nNOS with PSD-95 for 1 h before adding GST or GST-NR2B ...
[47]
Interaction of Nitric Oxide Synthase with the Postsynaptic Density ...
nNOS and PSD-95 are coexpressed in numerous neuronal populations, and a PSD-95/nNOS complex occurs in cerebellum. PDZ domain interactions also mediate binding ...Missing: paper | Show results with:paper<|control11|><|separator|>
[48]
The role of nitric oxide in pre-synaptic plasticity and homeostasis
In this review we focus on the role of NO in synaptic plasticity and specifically its function as a retrograde messenger.
[49]
Neuronal Nitric Oxide Synthase and Human Vascular Regulation
nNOS-derived NO plays an important role in the local regulation of basal microvascular tone as well as in the vasodilator response to mental stress.
[50]
The DLGAP family: neuronal expression, function and role in brain ...
Sep 4, 2017 · PSD-95 will in this work be referred to as DLG4 and GKAP/SAPAP1–4 will be referred to as DLGAP1–4. DLGAP chromosomal localisation, protein ...<|control11|><|separator|>
[51]
Wnt/β-catenin signaling stimulates the expression and synaptic ...
Mar 5, 2018 · Neuroligins mediate excitatory and inhibitory synapse formation: involvement of PSD-95 and neurexin-1β in neuroligin-induced synaptic ...Missing: trans- | Show results with:trans-
[52]
Destabilization of the Postsynaptic Density by PSD-95 Serine 73 ...
Dec 10, 2008 · Long-term potentiation (LTP) is accompanied by dendritic spine growth and changes in the composition of the postsynaptic density (PSD).Missing: knockout | Show results with:knockout
[53]
The Postsynaptic Adenomatous Polyposis Coli (APC) Multiprotein ...
Aug 18, 2010 · In our model, the APC complex provides retrograde signals via postsynaptic neuroligin that interacts extracellularly with presynaptic neurexin.