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Evolutionarily stable strategy

An evolutionarily stable strategy () is a behavioral or phenotypic strategy that, if adopted by the majority of members in a biologically interacting , cannot be invaded by any rare alternative strategy through , because the ESS provides a higher expected payoff to its possessors when interacting with others using the same strategy than the mutant does, or yields an equal payoff in such matchups but a strictly higher payoff when the ESS interacts with the mutant. The concept of an ESS was introduced in 1973 by evolutionary biologists and in their seminal paper analyzing the logic of animal conflicts, where they applied game-theoretic principles to explain why intraspecific contests often take the form of ritualized or low-risk "limited wars" rather than all-out fights to the death. Building on earlier ideas from and , such as Richard Lewontin's 1961 exploration of evolutionary games, Maynard Smith and Price formalized ESS as a refinement of Nash equilibria adapted to biological evolution, emphasizing stability under replicator dynamics where strategies spread proportional to their relative fitness. Maynard Smith further developed the framework in subsequent works, including the hawk-dove model (introduced with Price in 1973)—illustrating aggressive (hawk) versus peaceful (dove) tactics in resource disputes—and his influential 1982 book Evolution and the Theory of Games, which established ESS as a cornerstone of . Formally, a strategy I is an ESS if, for every alternative strategy J \neq I, the expected payoff E(I, I) > E(J, I), or if E(I, I) = E(J, I), then E(I, J) > E(J, J), where E(X, Y) denotes the fitness payoff of strategy X when interacting with Y. This condition ensures resistance to invasion by mutants, distinguishing ESS from mere Nash equilibria by incorporating evolutionary invasion criteria rather than just simultaneous optimization. Over time, the ESS framework has been linked to broader mathematical theories, including invasion fitness from adaptive dynamics and connections to , , and , allowing for analyses in structured populations or variable environments. ESS has been widely applied in biology to predict the evolution of diverse traits and behaviors, from sex ratios and parental investment to cooperation and altruism in social species. In behavioral ecology, it explains phenomena like the mixed hawk-dove equilibria in animal contests, where populations stabilize at proportions balancing risk and reward to maximize fitness. Extensions to multilevel selection have illuminated social insect societies, such as eusociality in ants and bees, where ESS models incorporate relatedness to resolve conflicts between individual and group interests. More recently, ESS analyses have integrated genomic data to study phenotypic plasticity and long-term evolutionary trajectories in fluctuating environments, such as predator-prey dynamics and host-parasite coevolution.

Fundamentals

Definition

An evolutionarily stable strategy (ESS) is a concept from that describes a behavioral which, when adopted by the majority of individuals in a , resists by alternative strategies that initially occur at low frequencies. In this context, a refers to a fixed behavioral rule or that determines an individual's actions in interactions with others, such as levels in conflicts or in social dilemmas. Payoffs represent the consequences of these interactions, typically measured by or survival rates, while invasion fitness quantifies the relative growth rate of a rare in a resident dominated by the ESS. The core property of an ESS is its resistance to : if nearly all members employ the , any mutant entering the at low abundance will have lower average than the resident , causing the mutant to decline over time. This stability arises from where strategies replicate proportionally to their ; higher-fitness strategies increase in frequency, while lower-fitness ones diminish. Informally, an ESS can be visualized as an in a where the resident outperforms or matches any challenger in pairwise comparisons, preventing evolutionary shifts even under pressures. Additionally, an ESS exhibits long-term stability under replicator dynamics, a model of evolutionary change where the frequency of each strategy evolves based on its relative payoff in the current population composition. In such dynamics, an ESS corresponds to an asymptotically stable state, meaning perturbations—such as the introduction of mutants—lead the population back to the original strategy distribution. This dynamic stability refines static concepts like the from classical , which identifies uninvadable strategies but does not inherently account for evolutionary processes over time.

Mathematical formulation

In the context of symmetric two-player games, the payoff structure is captured by a matrix A = (a_{ij}), where a_{ij} denotes the expected payoff to a player using pure strategy i against an opponent using pure strategy j. For mixed strategies, represented as probability distributions over the pure strategies, the expected payoff E(\mathbf{I}, \mathbf{J}) is the bilinear form \mathbf{I}^T A \mathbf{J}, where \mathbf{I} and \mathbf{J} are column vectors of probabilities. A strategy \mathbf{I} is an evolutionarily stable strategy (ESS) if, for every alternative strategy \mathbf{J} \neq \mathbf{I}, \text{either } E(\mathbf{I}, \mathbf{I}) > E(\mathbf{J}, \mathbf{I}) \quad \text{or} \quad \left[ E(\mathbf{I}, \mathbf{I}) = E(\mathbf{J}, \mathbf{I}) \ \text{and} \ E(\mathbf{I}, \mathbf{J}) > E(\mathbf{J}, \mathbf{J}) \right]. This condition ensures that \mathbf{I} cannot be invaded by any mutant strategy \mathbf{J} when the population predominantly consists of individuals using \mathbf{I}. The ESS criterion derives from invasion dynamics in finite populations, where a mutant strategy invades if its fitness exceeds that of the resident when rare. In a large population playing a symmetric game with payoff matrix A, the fitness of strategy i against resident \mathbf{I} is f_i = e_i^T A \mathbf{I}, with e_i the unit vector for i. For \mathbf{I} to resist invasion by any pure mutant j \neq i (or mixtures thereof), the first inequality requires a_{ii} > a_{ji} for all j \neq i in the pure case, preventing initial increase of the mutant; the second resolves ties by comparing payoffs in a mutant-vs-mutant matchup, ensuring the resident outperforms the mutant when both are rare. This pairwise stability condition extends to mixed strategies via linearity of expectations. Under the replicator , which model strategy frequency evolution in infinite populations, the system is given by \frac{dx_i}{dt} = x_i (f_i - \bar{f}), where x_i is the frequency of strategy i, f_i = (A \mathbf{x})_i is its , and \bar{f} = \mathbf{x}^T A \mathbf{x} is the average . An ESS \mathbf{I} corresponds to a monomorphic \mathbf{x} = \mathbf{I} that is asymptotically under these , as the barriers prevent deviations from growing. For pure strategies, a pure strategy i is an ESS if it satisfies the above condition against all alternatives, equivalent to i being a strict or a equilibrium satisfying the tie-breaking rule. For mixed strategies, a distribution \mathbf{p} is an ESS if \mathbf{p}^T A \mathbf{p} \geq \mathbf{q}^T A \mathbf{p} for all \mathbf{q} ( property) and, for any \mathbf{q} achieving equality, \mathbf{p}^T A \mathbf{q} > \mathbf{q}^T A \mathbf{q}. Polymorphic ESS arise as mixed strategies where multiple pure strategies coexist stably; under replicator dynamics, such an interior \mathbf{x}^* with support on strategies having equal is stable if it is an ESS, preventing by outsiders and ensuring frequencies do not drift to the boundary.

Historical Development

Origins and introduction

The concept of an evolutionarily stable strategy (ESS) was initially introduced by evolutionary biologist in 1972, with formal development occurring through his collaboration with mathematician in 1973. Their work was primarily motivated by the puzzle of animal conflict behaviors, where intra-species disputes often manifest as "limited wars" that avoid severe injury, rather than escalating to all-out fights that might yield higher individual rewards. This observation challenged prevailing explanations reliant on , prompting a search for mechanisms grounded in individual fitness advantages under . The collaboration originated from Price's prior contributions to understanding 's evolution, including his 1970 covariance equation that partitioned selection into individual and group components, which highlighted paradoxes in how seemingly selfless traits could spread despite individual costs. Influenced by W.D. Hamilton's theory, Price sought mathematical tools to reconcile these issues, drawing Maynard Smith's interest during the review of Price's unpublished 1968 manuscript on antlers, combat, and . Together, they adapted game-theoretic ideas to biological contexts, emphasizing strategies refined by rather than rational choice. Their foundational paper, "The Logic of Animal Conflict," appeared in Nature in November 1973, marking the first explicit definition of an ESS as a behavioral strategy that, when prevalent in a population, resists invasion by mutant alternatives through superior fitness in pairwise contests. To illustrate, they analyzed the hawk-dove game, modeling hawks as aggressive fighters willing to risk injury for resources and doves as ritualistic displayers that retreat to avoid harm; depending on the cost of injury relative to resource value, pure dove, pure hawk, or mixed ESS equilibria emerge as stable outcomes. This early formulation established ESS as a bridge between and , providing a conceptual tool later refined mathematically to predict long-term behavioral stability.

Key developments and contributors

Following the introduction of the evolutionarily stable strategy () concept, expanded its theoretical foundations in his 1982 book Evolution and the Theory of Games, where he adapted game-theoretic models to analyze evolutionary dynamics in biological populations, emphasizing applications to animal behavior and . This work synthesized earlier ideas and further developed models, such as the hawk-dove game, to illustrate how ESS predicts stable behavioral outcomes under . In 1984, Alan Grafen contributed a formal of ESS with theory, demonstrating that ESS criteria align with Hamilton's rule for by showing how strategies maximizing resist by mutants. Grafen's approach provided a rigorous population-genetic basis, proving that ESS equilibria correspond to optima under when relatedness effects are incorporated. During the and , Bernhard Thomas and colleagues advanced ESS theory to finite populations, addressing limitations of infinite-population assumptions by developing criteria for strategy stability under demographic ity and drift. Their 1981 model extended ESS to small groups, incorporating fixation probabilities to evaluate resistance in scenarios like territorial contests. Subsequent stochastic extensions by Thomas and others in the incorporated birth-death processes, revealing how noise in finite systems can destabilize classical ESS but favor robust strategies near neutral equilibria. A significant integration occurred in 1996 when Ulf Dieckmann and Robert Law linked ESS to adaptive dynamics, deriving a canonical equation for continuous trait evolution that treats ESS as local fitness maxima in phenotypic space. This framework, grounded in ecological processes, enabled analysis of coevolutionary trajectories, showing how ESS guides long-term in fluctuating environments without assuming rare .

Relation to Game Theory

Nash equilibrium overview

In , a Nash equilibrium is a strategy profile in which no player can improve their payoff by unilaterally deviating from their chosen strategy, assuming all other players maintain their strategies. This concept applies to normal-form games, where players select strategies simultaneously without knowledge of others' choices, and payoffs are determined by the combination of strategies selected. The notion was introduced by John Nash in his 1950 paper "Equilibrium Points in n-Person Games," published in the Proceedings of the National Academy of Sciences, where he defined equilibrium points for finite n-person games with pure strategies. Nash expanded this in his 1951 dissertation "Non-Cooperative Games," proving the existence of at least one equilibrium in mixed strategies for any finite game using a fixed-point theorem, thus establishing the concept's generality beyond zero-sum games. These works provided the foundational theorems ensuring equilibria exist under specified conditions, influencing subsequent developments in non-cooperative game theory. Nash equilibria rely on key assumptions, including rational players who maximize their expected payoffs, complete information where all players know the game's structure and payoffs, and one-shot interactions without repeated play or learning. These assumptions frame the equilibrium as a static solution to simultaneous-move games. To illustrate, consider a pure strategy equilibrium in the game, a coordination scenario with the following payoff matrix (row player payoffs first, column second):
StagHare
Stag(2, 2)(0, 1)
Hare(1, 0)(1, 1)
Here, both players choosing Stag and both choosing Hare are pure Nash equilibria, as neither benefits from unilateral deviation. For mixed strategies, the Matching Pennies game has no pure equilibrium but a mixed one, with payoffs:
Heads (P2)Tails (P2)
Heads (P1)(1, -1)(-1, 1)
Tails (P1)(-1, 1)(1, -1)
The equilibrium occurs when both players randomize equally (50% Heads, 50% Tails), making the opponent indifferent.

Distinctions from Nash equilibria

While a Nash equilibrium represents a fixed point in a strategic game where no rational player benefits from unilaterally deviating from their strategy given others' choices, an evolutionarily stable strategy (ESS) imposes a stronger condition: it must resist invasion by rare alternative (mutant) strategies in a population under frequency-dependent fitness selection, ensuring long-term persistence in evolutionary dynamics. This distinction arises because Nash equilibria assume perfect rationality and one-shot interactions, whereas ESS accounts for replicative processes where strategies spread based on relative success against the population's composition. Every ESS constitutes a Nash equilibrium in the underlying symmetric game, but the converse does not hold; ESS serves as a refinement by adding a second-order stability criterion against mutants when payoffs are tied. For example, in coordination games like a modified Stag Hunt—where coordinating on the high-reward "Stag" yields payoff 1 to both, but a "Hare" mutant also gets 1 against Stag while Hare-Hare yields 0—the (Stag, Stag) outcome is a Nash equilibrium since neither deviates profitably, yet it fails the ESS test because the equality in payoffs allows equal fitness for the mutant, and the tiebreaker condition (Stag vs. Hare payoff of 0 equals Hare vs. Hare of 0) is not strictly superior, permitting invasion. In contrast, strict Nash equilibria, where payoffs exceed those of any deviation, satisfy ESS automatically. A notable case involves risk-dominant versus payoff-dominant equilibria in 2x2 coordination games, both of which can be but differ in evolutionary viability; the risk-dominant equilibrium (characterized by a larger product of deviation losses, making it harder to escape) often emerges as the due to its broader resistance to perturbations, even if the payoff-dominant one offers higher average returns. For instance, in a with standard payoffs (Stag-Stag: 2; Hare-Hare: 1; cross: 0 for Stag, 1 for ), both pure strategies are and , but evolutionary processes favor the risk-dominant due to its larger basin of attraction. In evolutionary settings, replicator dynamics—where strategy frequencies evolve proportional to their current —further distinguish ESS by rendering them asymptotically stable fixed points, while non-ESS Nash equilibria may be unstable or neutrally stable, susceptible to drift or over time. This dynamic selection mirrors learning in populations, where ESS prevail as the outcomes resistant to strategy proliferation by alternatives, underscoring ESS's suitability for modeling biological or over mere static .

Variants and Extensions

Evolutionarily stable state

In evolutionary game theory, an evolutionarily stable state is defined as a population distribution over strategies that cannot be destabilized by small perturbations, such as rare mutations altering the relative frequencies of strategies in the population. This stability ensures that natural selection restores the original distribution following such changes, maintaining the state's persistence over evolutionary time. Unlike the concept of an evolutionarily stable strategy (ESS), which pertains to a single pure strategy dominating the population, an evolutionarily stable state accommodates broader configurations, including mixtures of strategies where no single type fixes completely.90201-1) Pure ESS serve as special cases of evolutionarily stable states, where the stable distribution concentrates entirely on one strategy, rendering it uninvadable by alternatives. However, evolutionarily stable states more generally permit protected polymorphisms, in which multiple distinct strategies coexist indefinitely at equilibrium frequencies, protected from displacement by mutants because the overall composition confers no fitness advantage to deviators. This allowance for polymorphism arises in scenarios with frequency-dependent selection, enabling diverse strategy sets to achieve collective stability that a monomorphic state might not. The mathematical conditions for an evolutionarily stable state extend the standard ESS criteria by incorporating second-order stability to address perturbations in the entire population distribution. While the first-order condition—analogous to the ESS invasion barrier—requires that the fitness of any rare mutant is lower than the resident state's average fitness, ensuring no initial invasion, it does not guarantee recovery from broader deviations. Second-order stability, in contrast, evaluates the curvature of the fitness landscape, demanding that small shifts away from the equilibrium generate restoring selective forces; for instance, in continuous strategy spaces, this often manifests as a negative second derivative of the invasion fitness function at the equilibrium point, promoting convergence back to the state.90201-1) Ilan Eshel formalized these ideas in 1983, distinguishing evolutionarily stable states through a framework emphasizing continuous in with continuously varying traits. Eshel's approach highlights that first-order ESS conditions alone may fail to prevent long-term drift under weak selection or affecting the whole , whereas his second-order criteria—requiring both uninvadability and dynamic restorability—ensure robustness against such distributed perturbations. This formalization, developed for haploid models with weak selection, underscores how evolutionarily stable states achieve viability selection beyond mere resistance, particularly enabling stable mixed distributions in polymorphic settings.90201-1)

Stochastic ESS

In stochastic environments, evolutionarily stable strategies () must account for randomness arising from , mutations, and environmental fluctuations in finite populations, where deterministic models often fail to capture real biological dynamics. A ESS is defined as a that remains stable in the long run against invasion by alternative strategies under such random perturbations, ensuring that the probability of fixation of mutants remains low even when is limited. This concept addresses the limitations of classical ESS by incorporating processes like the birth-death process, which models overlapping generations in finite populations of size N. Central to ESS is the fixation probability of a invading a resident , particularly under the . For a with relative r (the ratio of its payoff to the resident's), the probability \pi_I that a single fixes in a of size N is given by \pi_I = \frac{1 - 1/r}{1 - 1/r^N} when r \neq 1; otherwise, it is $1/N. A qualifies as a ESS if the fixation probability of any invading is less than or equal to $1/N, the neutral drift baseline, preventing long-term displacement by noise or rare variants. This formulation highlights how finite size amplifies drift, making stability dependent on N and r, unlike infinite- models. Nowak and May's work in the pioneered the analysis of stability in evolutionary games, demonstrating through simulations and models that noise can disrupt apparent equilibria in spatial and finite settings, leading to chaotic dynamics or shifts toward cooperation in games like the . Their studies emphasized how effects in finite populations alter the accessibility and persistence of strategies, providing a foundation for later analytical treatments. Building on the deterministic evolutionarily stable state as a precursor, incorporates these random elements to better model biological realism. In the weak selection limit, where fitness differences are small compared to baseline reproduction rates, conditions for stochastic ESS simplify to strategies that maximize expected payoffs against the resident population. Specifically, under weak selection \beta \to 0 (with \beta scaling payoff-to-fitness conversion), a strategy I is a stochastic ESS if its average payoff exceeds that of any mutant J when rare, approximated by \pi_I \approx 1/N + \beta ( \bar{\pi}_I - \bar{\pi}_J )/N, where \bar{\pi} denotes stationary frequencies. This limit reveals that neutral stability under drift requires payoff dominance, filtering noise and favoring robust strategies in fluctuating environments. Such conditions have been derived for the frequency-dependent , ensuring long-run stability without requiring strict inequality in fitness.

Applications and Examples

Classic biological models

One of the foundational applications of the evolutionarily stable strategy (ESS) concept in biology is the hawk-dove game, which models agonistic interactions over resources such as territories or mates in animal populations. In this , two strategies are considered: "," characterized by escalated fighting until victory or severe injury, and "dove," involving non-injurious displays followed by retreat if the opponent escalates. The payoffs depend on the value V of the contested resource (e.g., a feeding or breeding site) and the cost C of injury from fighting, where typically C > V > 0. The payoff structure can be represented in the following , assuming baseline normalized to zero without contest:
Opponent \ PlayerDove
\frac{V - C}{2}V
Dove$0\frac{V}{2}
When two hawks meet, each has a 50% chance of winning the but incurs with probability 1, yielding an expected payoff of \frac{V - C}{2}. A hawk against a dove secures the full V without , as the dove retreats. Dove versus dove results in equal sharing of V, giving \frac{V}{2} each, while a dove against a hawk receives nothing. Analysis shows that if V < C, neither pure strategy is an ESS: a population of pure hawks is vulnerable to invasion by doves (due to the high risk of injury), and pure doves to hawks (who exploit without cost). The ESS is a mixed strategy where individuals play hawk with probability p = \frac{V}{C}, balancing aggression such that the expected payoffs equalize, preventing invasion by alternative strategies. This predicts peaceful resolutions in conflicts, as doves' displays often suffice when resource value is less than injury cost, reducing overall escalation and injury rates in the population. The hawk-dove model has been applied to territoriality and mating behaviors in birds and mammals, with empirical validations supporting its predictions. In birds, such as (Erythrura gouldiae), red-headed morphs act as hawks (aggressively defending nest sites), while black-headed morphs behave as doves (retreating from conflicts); the stable polymorphism aligns with ESS proportions where aggression is costly relative to breeding resources. Maynard Smith validated these patterns through comparative analyses of agonistic behaviors across species, noting that dove-like signaling evolves to resolve contests without injury in both avian territorial defense and mammalian mating rivalries. Another classic model is the war of attrition, which extends ESS analysis to contests settled by persistence rather than immediate escalation. Here, opponents display costly signals (e.g., prolonged threats) over time, with the first to withdraw conceding the resource V, while both incur accumulating costs (e.g., energy expenditure at rate c per unit time). The ESS involves a mixed strategy of persistence times, often exponentially distributed, where individuals signal commitment calibrated to V and c, leading to efficient resolution through asymmetric withdrawal without full fights. This framework explains observed signaling durations in animal disputes, such as prolonged roaring in red deer stags during mating season.

Prisoner's dilemma in evolutionary contexts

The is a canonical game in evolutionary game theory, characterized by a payoff structure where the temptation to (T) exceeds the reward for mutual (R), which in turn exceeds the punishment for mutual defection (P), and the sucker's payoff for unilateral cooperation (S) is the lowest, satisfying T > R > P > S and 2R > T + S. In a one-shot PD, defection is the only , as any population of cooperators can be invaded by defectors, leading to the in biological contexts. However, in evolutionary settings with repeated interactions, conditional strategies enable cooperation to become an by leveraging the shadow of the future, where the probability of future encounters is sufficiently high. In iterated PD, Robert Axelrod's computer tournaments in the demonstrated that tit-for-tat—a strategy that cooperates on the first move and then mirrors the opponent's previous action—outperformed other strategies, including always-defect and always-cooperate, across diverse rule sets and participant submissions. Tit-for-tat achieves evolutionary stability when the future is valued enough (e.g., discount factor w > (T - R)/(T - P)), resisting invasion by defectors through retaliation while forgiving errors to restore , making it robust in noisy environments. Other conditional strategies, such as (win-stay, lose-shift), also emerge as ESS candidates in similar iterated frameworks, promoting long-term reciprocity. These findings underscore how reciprocity mechanisms resolve the PD dilemma, allowing to evolve without . Evolutionary stability in PD further arises through spatial structure and punishment mechanisms, where clustering of cooperators protects against defector invasion. In spatial models, individuals interact primarily with neighbors on a lattice; Nowak and May showed that cooperators form dynamic clusters, sustaining via local mutual benefit despite global defection pressures, leading to chaotic but persistent patterns of . Punishment strategies, such as altruistic punishment where cooperators impose costs on defectors, can also stabilize as an ESS by making defection costly, as seen in extensions of tit-for-tat with punitive responses. Biological examples illustrate these principles in natural systems. In social insects like and bees, worker cooperation in foraging and defense resembles iterated PD, where conditional strategies akin to tit-for-tat—cooperating with nestmates but punishing intruders—maintain as an ESS through repeated interactions and spatial proximity within colonies. Similarly, microbial cooperation in biofilms, such as producing shared extracellular polymers, faces PD-like public goods dilemmas; spatial clustering limits cheater spread, while metabolic punishment (e.g., via costly signaling) enforces ESS cooperation during and .

Applications to human behavior

Evolutionarily stable strategies (ESS) have been extended to , where memes and social norms function as heritable strategies transmitted through learning rather than . In this framework, cultural variants can achieve stability if they resist invasion by alternative norms under biased transmission mechanisms, such as conformist or success-based copying. Pioneering models by Robert Boyd and Peter Richerson in the 1980s demonstrated how can lead to group-beneficial norms, even when individually costly, by incorporating concepts into dual-inheritance theory. For instance, their 1990 analysis showed that selection among groups practicing alternative can favor cooperative cultural strategies over selfish ones, provided migration and within-group variation are limited. Applications to human altruism illustrate this in small-scale societies, such as hunter-gatherers, where costly cooperative behaviors toward non-kin may persist as if intergroup conflict imposes differential costs on defectors. Samuel Bowles' 2009 model, calibrated to ethnographic data from groups like the Ache and Hiwi, indicates that warfare mortality rates of 12-14% could stabilize parochial —cooperation within the group paired with toward outsiders—as an , with benefits outweighing costs when group productivity advantages exceed 1-3%. In economic contexts, costly signaling models apply to behaviors like or generosity, where honest signals of quality or intent are evolutionarily stable only if fakers incur prohibitive costs. Alan Grafen's 1990 formalized this, showing that signals (e.g., extravagant displays in markets or competitions) evolve as when their cost correlates with the signaller's underlying , influencing human economic decisions from job credentials to charitable giving. Critiques highlight limitations of standard ESS in capturing , as cultural transmission via learning introduces rapid and that outpace genetic fixation, potentially destabilizing gene-based equilibria. Joseph Henrich's work in the 2000s and 2010s argues that reliance on genetic overlooks how amplifies through accumulated knowledge and norms, rendering pure genetic models insufficient for explaining large-scale human prosociality. For example, in his 2015 , Henrich emphasizes that biases enable behaviors like norm enforcement that are not genetically hardwired but evolve as stable strategies in social ecologies, challenging the assumption of slow, replicator dynamics in human contexts. Modern applications in use laboratory experiments to test ESS predictions, often adapting evolutionary dynamics to finite populations with learning rules mimicking cultural transmission. Similarly, experiments on Rock-Paper-Scissors analogs reveal that mixed strategies predicted as ESS emerge robustly, even with human subjects using best-response learning, validating ESS as a for strategic in economic settings.

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