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Lipofectamine

Lipofectamine is a family of proprietary cationic lipid-based transfection reagents developed and commercialized by Invitrogen (now Thermo Fisher Scientific) for the efficient delivery of nucleic acids, such as plasmid DNA, siRNA, and mRNA, into eukaryotic cells.^[1] Launched in 1993 as a first-generation product, it builds on the foundational lipofection technology pioneered by Philip L. Felgner and colleagues in 1987, which introduced synthetic cationic lipids for non-viral gene transfer.^[1]^[2] The core mechanism of Lipofectamine involves the formation of lipoplexes—electrostatic complexes between the positively charged lipids and negatively charged nucleic acids—that promote cellular uptake primarily through endocytosis, followed by endosomal escape and release into the cytoplasm for subsequent nuclear translocation or translation.^[3]^[4] The original formulation consists of a 3:1 (w/w) mixture of the polycationic lipid 2,3-dioleyloxy-N-[2(sperminecarboxamido)ethyl]-N,N-dimethyl-1-propanaminium trifluoroacetate (DOSPA) and the helper lipid dioleoyl phosphatidylethanolamine (DOPE), enabling high transfection efficiency in a variety of cell lines with relatively low cytotoxicity.^[3]^[1] Over time, the Lipofectamine portfolio has evolved to address broader applications and challenging cell types. Lipofectamine 2000, an improved second-generation reagent, enhances compatibility with both adherent and suspension cells, supports co-transfection of siRNA and plasmid DNA, and performs effectively in serum-containing media without requiring media changes, making it ideal for high-throughput RNAi and gene expression studies.^[5] Lipofectamine 3000 represents the latest advancement, incorporating lipid nanoparticle technology and an optional P3000 enhancer reagent to achieve superior transfection rates (up to 75% in difficult cells like primary neurons), reduced toxicity, and versatility for genome editing tools such as CRISPR/Cas9, while maintaining reproducibility across established cell lines, stem cells, and hard-to-transfect primary cells.^[6] Specialized variants, like Lipofectamine Stem, further optimize delivery for human pluripotent stem cells in gene editing workflows.^[7] Widely adopted in molecular and cell biology, Lipofectamine reagents have been cited in tens of thousands of publications for applications including transient and stable gene expression, functional genomics, drug discovery, and therapeutic nucleic acid delivery research.^[1] Their non-viral nature offers advantages over viral vectors, such as ease of use, scalability, and avoidance of immunogenicity, though optimization is often required for specific cell types and nucleic acid cargos.^[8]

Development

Invention

Lipofectamine was developed by researchers at Life Technologies, Inc., in the early 1990s as a cationic lipid-based system designed for the delivery of nucleic acids into eukaryotic cells.^[9] It was first described by P. Hawley-Nelson, V. Ciccarone, G. Gebeyehu, J. Jessee, and P.L. Felgner in a 1993 publication.^[9] This innovation emerged at Life Technologies, Inc., where researchers developed formulations using polycationic lipids to form liposomes that complex with DNA or RNA, enabling efficient cellular uptake without viral vectors. The development of Lipofectamine addressed key limitations of earlier non-viral transfection methods, such as calcium phosphate co-precipitation, which was introduced in the 1970s but suffered from inconsistent efficiency, high variability due to pH sensitivity, and potential cytotoxicity in sensitive cell types.^[10] Unlike electroporation, which required expensive equipment and often caused cell membrane damage, Lipofectamine offered a simpler, reagent-based approach for broad applicability in eukaryotic cells, prioritizing non-viral safety and reproducibility.^[11] The composition of Lipofectamine, including the 3:1 (w/w) mixture of DOSPA and DOPE, was first detailed in the 1993 publication.^[9] Early demonstrations in the 1990s highlighted Lipofectamine's superior performance compared to prior liposomal systems like Lipofectin, as described in initial studies and technical reports.^[12] For instance, the 1993 technical report described its formulation as a 3:1 (w/w) mixture providing markedly improved gene expression levels over prior liposomal systems like Lipofectin.^[12]

Commercialization

Lipofectamine was introduced by Invitrogen in 1993 as a ready-to-use cationic lipid-based transfection reagent designed to simplify nucleic acid delivery into eukaryotic cells.^[13] This launch marked a significant advancement in providing researchers with an efficient, off-the-shelf solution for in vitro transfection, eliminating the need for custom liposome preparation.^[14] Initial marketing efforts by Invitrogen positioned Lipofectamine as a gold standard for in vitro transfection in molecular biology laboratories, with detailed protocols released in the mid-1990s to guide its use in DNA and RNA delivery experiments.^[15] These protocols emphasized its broad applicability across cell types, contributing to its rapid integration into standard lab workflows. Invitrogen's product portfolio, including Lipofectamine, underwent significant corporate changes through mergers and acquisitions. In 2000, Invitrogen acquired the original Life Technologies, though the Lipofectamine brand remained under Invitrogen's development.^[16] A pivotal merger occurred in 2008 when Invitrogen combined with Applied Biosystems to form Life Technologies Corporation, bringing Lipofectamine under the new entity's umbrella.^[17] In 2014, Thermo Fisher Scientific acquired Life Technologies for $13.6 billion, integrating Lipofectamine into its broader life sciences solutions and rebranding the parent company while retaining the product's legacy.^[18] By the late 1990s, Lipofectamine had achieved widespread adoption in academic and biotechnology research, becoming a staple reagent cited in thousands of publications for its reliable performance in gene expression studies.^[13] This early market impact solidified its role as an essential tool, with over tens of thousands of citations accumulated since its introduction, reflecting its enduring influence on transfection methodologies.^[13]

Composition

Original Formulation

The original formulation of Lipofectamine consists of a 3:1 (w/w) liposome mixture of the polycationic lipid DOSPA, chemically known as 2,3-dioleyloxy-N-[2(sperminecarboxamido)ethyl]-N,N-dimethyl-1-propanaminium trifluoroacetate, and the neutral co-lipid DOPE, or dioleoylphosphatidylethanolamine.^[14]^[3] This reagent is supplied as a 2 mg/mL solution in membrane-filtered water.^[19] Upon dilution for use, the solution forms milky suspensions characteristic of liposome aggregation into multilamellar vesicles.^[20] For optimal stability, the original Lipofectamine is recommended to be stored at 4°C in a sealed container, where it remains viable for up to 12 months without freezing.^[1]

Key Components

Lipofectamine's formulation primarily consists of the cationic lipid DOSPA (2,3-dioleyloxy-N-[2-(sperminecarboxamido)ethyl]-N,N-dimethyl-1-propanaminium) and the helper lipid DOPE (dioleoylphosphatidylethanolamine).^[21]^[22] DOSPA serves as the key cationic component, imparting a positive charge that electrostatically binds to negatively charged nucleic acids, facilitating their condensation and packaging into lipoplexes; its spermine headgroup enhances DNA condensation efficiency due to the multivalent interactions mimicking natural polyamine-DNA binding.^[23]^[22] DOPE, a neutral fusogenic lipid, promotes membrane destabilization and fusion by adopting an inverted hexagonal (H_II) phase structure, which aids in the disruption of endosomal membranes during intracellular delivery.^[21]^[24] The synergy between DOSPA and DOPE is critical: DOSPA enables the initial electrostatic complexation with nucleic acids, while DOPE supports subsequent endosomal escape through its phase behavior, optimizing overall transfection performance.^[21] This combination exhibits a low cytotoxicity profile relative to earlier cationic lipids, with typical cell viability exceeding 80% in standard transfection protocols across various cell types.^[25]^[14]

Mechanism of Action

Lipoplex Formation

Lipofectamine, a cationic lipid formulation, forms lipoplexes through electrostatic interactions between its positively charged liposomes and the negatively charged phosphate backbone of anionic nucleic acids such as DNA or RNA. This binding drives the spontaneous condensation of nucleic acids into compact complexes, where the cationic headgroups of lipids like DOSPA neutralize the polyanionic charges, resulting in stable lipid-nucleic acid assemblies known as lipoplexes. These interactions are the primary force behind complex formation, enabling efficient packaging without covalent linkages.^[26]^[8] Optimal complexation requires specific ratios of Lipofectamine reagent to nucleic acid to balance charge neutralization and avoid excess free lipid, which can lead to toxicity or instability. Standard protocols recommend 2-3 µL of Lipofectamine 2000 per 1 µg of DNA for most mammalian cell lines, though this can vary from 1.5-4 µL per µg depending on cell type and nucleic acid amount; for example, in a 24-well plate, 1.0-2.5 µL is used with 0.5 µg DNA. These ratios ensure a net positive charge on the lipoplex surface, facilitating subsequent cellular interactions while promoting efficient encapsulation.^[27] The resulting lipoplexes typically exhibit a multilamellar vesicle structure, consisting of concentrically arranged lipid bilayers sandwiching layers of nucleic acids, with overall sizes ranging from 100-300 nm in diameter. This architecture protects the encapsulated nucleic acids from degradation by extracellular nucleases, enhancing stability during delivery. Dynamic light scattering analyses confirm these nanoscale dimensions, which are influenced by the lipid-to-nucleic acid ratio and preparation conditions, contributing to the complexes' ability to evade rapid clearance in biological media.^[28] In practice, lipoplex formation follows a straightforward protocol: the Lipofectamine reagent is diluted in serum-free medium such as Opti-MEM, combined with the nucleic acid solution at the optimal ratio, and incubated for 5-20 minutes at room temperature to allow complete complexation. This brief assembly step occurs in the absence of serum to prevent interference from proteins, yielding ready-to-use lipoplexes that can then be applied directly to cells. Variations in incubation time, such as up to 30 minutes in optimized formulations, further refine size uniformity and transfection potential.^[29]

Cellular Uptake and Delivery

Lipoplexes formed by Lipofectamine are primarily internalized by cells through endocytic pathways, including clathrin-mediated and caveolae-mediated endocytosis, although direct fusion with the plasma membrane can also occur in certain cell types.^[15]^[30] This uptake process allows the lipoplexes to enter the cell without causing significant membrane disruption initially, facilitating their transport to early endosomes. Unlike some other delivery systems, Lipofectamine-based lipoplexes exhibit efficient avoidance of microtubule-dependent intracellular trafficking, which may contribute to their rapid progression through the endosomal pathway.^[4] Once in the endosomes, the acidic environment triggers endosomal escape, a critical step for releasing the nucleic acid cargo into the cytoplasm. The helper lipid dioleoylphosphatidylethanolamine (DOPE) in Lipofectamine formulations plays a key role here; its protonation in the low-pH endosome promotes a transition to a hexagonal phase, destabilizing the endosomal membrane and enabling the lipoplex to disrupt it for cargo release.^[31] This mechanism ensures that the nucleic acids are liberated efficiently from the endolysosomal compartment, minimizing degradation by lysosomal enzymes. For DNA delivery, the released plasmid must further traffic to the nucleus for transgene expression, which typically requires cell division to allow nuclear envelope breakdown and entry during mitosis.^[32] In highly transfectable cell lines such as HEK293, this process can achieve transfection efficiencies up to 80%, highlighting the effectiveness of Lipofectamine in supportive cellular contexts.^[33] In contrast, RNA delivery—such as mRNA for translation or siRNA for gene silencing—relies solely on cytoplasmic release, as these molecules do not require nuclear entry to exert their effects.^[30]

Applications

DNA Transfection

Lipofectamine is primarily utilized for the transient or stable expression of plasmid DNA encoding reporter genes, such as green fluorescent protein (GFP) or luciferase, in mammalian cell lines to facilitate gene function analysis and protein production.^[34] This non-viral method has become a cornerstone in cell biology research, enabling the introduction of exogenous DNA without the need for viral vectors.^[2] The technique supports both short-term expression for immediate phenotypic observation and long-term integration for stable cell line generation, marking a significant advancement in non-viral gene delivery since its foundational development.^[2] Transfection efficiency with Lipofectamine is optimized for adherent mammalian cells, such as HeLa and Chinese hamster ovary (CHO) lines, where rates typically range from 20% to 70% depending on cell type, DNA quality, and optimization parameters.^[35] High cell density at 70-90% confluence enhances uptake, with protocols recommending plating 0.5-2 × 10^5 cells per well in a 24-well plate.^[34] A standard procedure involves diluting 0.5-2 µg of plasmid DNA in serum-free medium like Opti-MEM, mixing with Lipofectamine at a 1:2 to 1:3 DNA (µg):reagent (µl) ratio, and incubating for 20 minutes to form lipoplexes before adding to cells.^[34] The complexes are typically incubated with cells for 4-6 hours in serum-free conditions, after which complete growth medium can be added to minimize toxicity while maintaining expression.^[34] Successful DNA transfection via Lipofectamine enables diverse applications, including the study of gene regulation, signaling pathways, and recombinant protein expression for biochemical assays.^[36] For instance, transient expression of luciferase reporters allows quantification of promoter activity, while stable integration in CHO cells supports high-yield protein production in biomanufacturing.^[34] This approach, pioneered in the late 1980s, revolutionized non-viral gene therapy research by providing a reproducible alternative to earlier methods like calcium phosphate precipitation, achieving 5- to over 100-fold higher efficiency in various mammalian lines.^[2]

RNA Delivery

Lipofectamine reagents facilitate the delivery of RNA molecules into cells, enabling transient functional studies without altering the genome. These cationic lipid-based formulations form complexes with RNA, promoting cellular uptake and subsequent biological activity. For RNA interference (RNAi) applications, Lipofectamine RNAiMAX is particularly optimized, providing high efficiency with reduced optimization needs across diverse cell types. In siRNA and miRNA delivery, Lipofectamine enables gene silencing by incorporating the RNA into the RNA-induced silencing complex (RISC), which targets and degrades complementary mRNA. Typical protocols use siRNA concentrations of 10-50 nM, achieving 70-90% target gene knockdown within 24-48 hours post-transfection in mammalian cell lines. This efficiency supports precise functional genomics, with minimal off-target effects when using low doses and validated siRNA designs.^[37]^[38] For mRNA delivery, Lipofectamine MessengerMAX promotes rapid translation in the cytoplasm, yielding detectable protein expression within hours and avoiding genomic integration risks associated with DNA vectors. Doses of 0.5-2 μg mRNA per well in 96-well plates often result in robust, homogeneous expression suitable for protein function studies. This approach has been instrumental in vaccine development, where mRNA transfection in antigen-presenting cells simulates immune responses for preclinical evaluation.^[39]^[40]^[41] RNA delivery with Lipofectamine generally exhibits lower toxicity compared to DNA transfection, as it bypasses nuclear entry and reduces cellular stress, with cell viability often exceeding 80-90% at optimal conditions. Reagents like RNAiMAX are formulated for minimal cytotoxicity, making them suitable for sensitive applications. They are particularly effective in hard-to-transfect cells, such as primary neurons, where efficiencies reach up to 83% for siRNA uptake with low neuronal damage.^[42]^[43] Key applications include high-throughput screening for gene function, where Lipofectamine supports automated RNAi assays in 96- or 384-well formats to identify essential genes or drug targets. Additionally, it enables CRISPR-Cas9 ribonucleoprotein (RNP) delivery, with Lipofectamine CRISPRMAX achieving editing efficiencies of 55-85% in various cell lines, facilitating precise genome modifications without persistent nuclease expression.^[44]

Variants

Lipofectamine 2000

Lipofectamine 2000 was introduced in the early 2000s by Invitrogen (now part of Thermo Fisher Scientific) as an advanced cationic lipid-based transfection reagent, offering enhanced transfection efficiency and reduced cytotoxicity compared to the original Lipofectamine formulation.^[45] This variant was designed to address limitations in transfecting challenging cell types, such as primary and post-mitotic cells, while maintaining a straightforward, serum-compatible protocol that minimizes the need for specialized media or additives.^[34] Its development marked a significant step forward in non-viral gene delivery tools, enabling broader applicability in molecular biology research.^[45] The formulation of Lipofectamine 2000 consists of a proprietary blend of cationic lipids, which carry positively charged head groups and hydrophobic hydrocarbon tails, combined with neutral helper lipids that promote fusogenic properties to facilitate endosomal escape.^[45] These components self-assemble into unilamellar liposomes approximately 100 nm in diameter when prepared in aqueous solutions.^[45] The reagent is supplied as a 1 mg/mL solution in water, stable at 2–8°C, and forms lipoplexes with nucleic acids through electrostatic interactions between the cationic lipids and the negatively charged phosphate backbone of DNA or RNA.^[46] This composition enhances stability and cellular interaction while reducing aggregation issues seen in earlier reagents.^[45] In terms of performance, Lipofectamine 2000 demonstrates up to 10-fold higher transfection efficiency than the original Lipofectamine in primary cells, including post-mitotic neurons, where it achieves 20–30% transfection rates compared to less than 3% previously.^[45] It is particularly effective for delivering both DNA and siRNA, supporting gene expression studies and RNA interference applications with low toxicity across a wide range of adherent mammalian cell types.^[45] Additionally, its compatibility with high-throughput formats, such as 96-well plates, allows for scalable transfections in multi-well assays without compromising efficiency or cell viability.^[45] A seminal 2004 study validated these enhancements, demonstrating Lipofectamine 2000's efficacy in primary rat hippocampal and cortical neurons, where it enabled robust transgene expression and siRNA-mediated knockdown in 96-well formats suitable for high-throughput screening.^[45] The research highlighted its utility in challenging models like primary hepatocytes, underscoring improvements in nuclear delivery and overall transfection success rates.^[45]

Lipofectamine 3000 and Later Versions

Lipofectamine 3000, introduced in 2014 by Thermo Fisher Scientific, represents a significant advancement in transfection reagents through its formulation of advanced lipid nanoparticles combined with the P3000 enhancer reagent. This combination enables superior delivery of plasmid DNA, achieving over 70% transfection efficiency in challenging cell types, including induced pluripotent stem cells (iPSCs) and other hard-to-transfect lines, while maintaining high cell viability.^[47]^[48]^[49] Lipofectamine RNAiMAX is a specialized variant optimized for RNA interference applications, particularly the delivery of small interfering RNA (siRNA) and microRNA (miRNA) mimics or inhibitors. It provides high transfection efficiency across a wide range of cell types with minimal cytotoxicity, which supports the use of lower reagent concentrations to reduce potential off-target effects associated with RNA knockdown experiments.^[50]^[51] Lipofectamine MessengerMAX, launched in late 2014, is tailored for mRNA transfection and delivers exceptional protein expression levels in primary cells and neurons, often outperforming traditional DNA-based methods by up to fivefold in these sensitive cell types. This reagent facilitates rapid, transient gene expression without genomic integration, making it ideal for studying protein function in hard-to-transfect primary cultures.^[52]^[39] Among later variants, Lipofectamine CRISPRMAX, developed around 2016, enhances the delivery of Cas9 ribonucleoprotein (RNP) complexes for CRISPR-Cas9 genome editing, offering improved editing efficiency in diverse mammalian cell lines compared to earlier lipid formulations.^[44]^[53] Lipofectamine Stem, introduced in 2018, is optimized for transfecting human pluripotent stem cells with minimal differentiation, supporting gene editing workflows in stem cell research.^[54] Overall, these post-2014 iterations reflect a broader trend in Lipofectamine development toward formulations with lower toxicity profiles and expanded compatibility across cell types, including primary and stem cells, to support advanced genomic and transcriptomic research.

Advantages and Limitations

Benefits

Lipofectamine reagents demonstrate high transfection efficiency, typically achieving 50-90% in standard cell lines such as HEK 293 and HeLa, with Lipofectamine 3000 reaching over 70% in many cases.^[55]^[48] This efficiency is particularly notable in hard-to-transfect cells, where it can be up to 10-fold higher than earlier formulations like Lipofectamine 2000, while maintaining superior cell viability compared to electroporation methods that often compromise survival due to physical stress on cells.^[48]^[56] The versatility of Lipofectamine extends to a broad spectrum of nucleic acids, including plasmid DNA, mRNA, siRNA, and genome-editing tools like CRISPR-Cas9 and TALENs, enabling effective delivery across diverse applications.^[48]^[34] It performs reliably in a wide array of cell types, from common immortalized lines like CHO and Jurkat to challenging primary cells such as neurons, stem cells, and hepatocytes, without requiring cell-specific optimizations in many protocols.^[39]^[48] Lipofectamine offers exceptional ease of use through a straightforward, non-viral protocol that involves simple mixing of reagents with nucleic acids and addition to cells, requiring no specialized equipment beyond standard lab incubators.^[43] This approach ensures high reproducibility and scalability, supporting high-throughput screening in multi-well formats for rapid experimental workflows.^[43]^[48] As a commercially available product from Thermo Fisher Scientific, Lipofectamine is cost-effective, with reaction costs as low as pennies and a shelf life of up to 12 months when stored properly at 4°C, facilitating accessible and efficient gene delivery in research settings worldwide.^[48]^[57] Its widespread adoption underscores its role in enabling quick, reliable transfections without the need for custom synthesis or extensive preparation.^[58]

Drawbacks

Lipofectamine exhibits significant cell-type dependency, with transfection efficiencies often below 20% in challenging cell types such as non-adherent cells, primary immune cells, and mesenchymal stem cells (MSCs), necessitating extensive protocol optimization for each application.^[59] For instance, in human adipose-derived MSCs, optimized conditions achieved only 23.75% efficiency, while unoptimized protocols yield even lower results, highlighting the reagent's variable performance across cell lines and primary cultures.^[59] This dependency arises from differences in cell membrane properties and uptake mechanisms, limiting its broad applicability without tailored adjustments.^[60] Toxicity represents a key limitation of Lipofectamine, manifesting as dose-dependent cytotoxicity that compromises cell viability, particularly at higher concentrations required for efficient transfection.^[42] In various cell types, including pancreatic cancer cells and endothelial cells, elevated doses (e.g., >2 µL of Lipofectamine LTX or 6 µL of Lipofectamine 2000) lead to reduced viability ranging from 20-55%, with effects including membrane disruption and apoptosis that persist post-transfection.^[61]^[40] This cytotoxicity can confound experimental outcomes by altering cell health and gene expression profiles, often requiring careful dose titration to balance delivery efficacy and survival.^[42] The transient nature of Lipofectamine-mediated transfection further restricts its utility, as transgene expression typically peaks within 12-48 hours and declines significantly over days to weeks, rendering it unsuitable for applications demanding stable, long-term genetic modifications without additional selection strategies.^[59] In primary cells like MSCs, expression levels drop markedly by 48 hours post-transfection, limiting studies to short-term effects unless integrated with stable integration methods.^[59] Due to these drawbacks, alternatives such as viral vectors are preferred for in vivo applications owing to their higher efficiency and stability, while electroporation or nucleofection offer superior performance in primary and hard-to-transfect cells, achieving up to 90% efficiency with reduced chemical toxicity despite some cell death (35-45%).^[62]^[63] Later variants like Lipofectamine 3000 have partially addressed toxicity through formulation improvements, but cell-type challenges persist.^[42]

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Jan 16, 2014 · Lipofectamine 3000 demonstrates the highest transfection efficiency in the broadest spectrum of cell types with improved cell viability and reproducibility.
[52]
Lipofectamine 3000 Transfection Reagent - Thermo Fisher Scientific
Lipofectamine 3000 Transfection Reagent delivers a high efficiency for hard-to-transfect cell lines. You can achieve over 70% transfection efficiency in a cost ...
[53]
Generation and Transfection of Induced Pluripotent Stem Cells ...
Lipofectamine 3000 Transfection Reagent was developed to improve transfection efficiency in difficult-to-transfect cells. In this study, we demonstrated that ...
[54]
Lipofectamine RNAiMAX Transfection Reagent—In Vitro Delivery of ...
6–10 day deliveryLipofectamine RNAiMAX exhibits minimal transfection-mediated cytotoxicity. Transfection-mediated cytotoxicity can mask the true phenotype of a target gene ...Missing: miRNA | Show results with:miRNA
[55]
Transfecting Stealth using Lipofectamine® RNAiMAX - US
Lipofectamine® RNAiMAX is a proprietary formulation specifically developed for the transfection of siRNA and Stealth™ RNAi duplexes into eukaryotic cells.Missing: off- target
[56]
Thermo Fisher Scientific Targets Neurons and Primary Cells with ...
Nov 4, 2014 · MessengerMax is the newest high-efficiency transfection reagent in Thermo Fisher's portfolio since introducing Lipofectamine 3000 in January.Missing: features | Show results with:features
[57]
Lipofectamine™ CRISPRMAX™ Cas9 Transfection Reagent 0.75 mL
In stock $86 deliveryContains one vial of Lipofectamine CRISPRMAX Reagent and one vial of Cas9 Plus Reagent. Store at 2–8°C. Do not freeze.
[58]
Use of shRNA for Stable Suppression of Chemokine Receptor ... - NIH
Our transfection efficiency is 50–90 % on transient transfections and 50–80 ... For lipid based transfections-our lab has successfully used Lipofectamine 2000, ...
[59]
Improved Transfection Efficiency and Metabolic Activity in Human ...
Electroporation method shows high transfection efficiency, but cell survival rate is low due to cell damage during transfection (10, 16, 20).
[60]
Lipofectamine™ LTX Reagent - FAQs - Thermo Fisher Scientific
What is the stability of your transfection reagents? Our transfection reagents are shipped under ambient conditions and should be stored at 4 degrees C ...Missing: original | Show results with:original
[61]
Lipid-based Transfection Reagents Exhibit Cryo-induced Increase in ...
Mar 8, 2016 · One of the most widely used commercially available lipid-based transfection reagent to date is Lipofectamine 2000 (LF2000), well-known for its ...
[62]
Optimizing Lipofectamine LTX Complex and G-418 Concentration ...
The purpose of this study was to evaluate and suggest the optimum quantities of lipoplex components to enhance the transfection efficiency of human adipose ...
[63]
Comparative Evaluation of Lipofectamine and Dendrimer for ... - NIH
Our results showed significantly better efficiencies of Lipofectamine compared to PAMAM dendrimer for short RNA transfection in both cell types.Missing: profile DOSPA
[64]
Toxicity of cationic liposome Lipofectamine 2000 in human ...
Aug 6, 2025 · On the other side, since Lipofectamine presents a high cytotoxicity for the cell viability despite its robust and high transfection efficiency ...
[65]
Transfection types, methods and strategies: a technical review - PMC
In contrast, liposomal-based reagents such as Lipofectamine and DharmaFECT families were reported to produce higher transfection efficiencies than non- ...
[66]
146. Alternative Methods for Ex Vivo Gene Transfer to Primary ...
The gene transfer procedure caused the most cell death for the lipofectamine method (~45%), followed by nucleofection (~35%), and viral vector (5–10% in each ...