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Solution

A solution is a homogeneous composed of two or more substances, in which a solute is dissolved in a , resulting in a uniform composition that cannot be distinguished into separate components by the . The constitutes the major component, typically the substance present in the greatest amount, while the solute is the minor component that disperses evenly throughout the . Solutions play a central role in and , as many chemical reactions, including those in biological systems and , occur within them. They exhibit properties such as in form and the inability to separate by , distinguishing them from heterogeneous mixtures like suspensions. Common examples include saltwater ( in ), sweetened beverages ( in ), and the air we breathe (a gaseous solution of , oxygen, and other gases). Solutions can be classified by the physical states of their components—gaseous (e.g., air), liquid (e.g., saltwater), solid (e.g., alloys like brass, a solid solution of copper and zinc)—and further categorized as unsaturated (able to dissolve more solute), saturated (at maximum solute capacity at a given temperature), or supersaturated (holding more solute than normally possible). Their concentration, which quantifies the amount of solute per unit of solution, is expressed in units like molarity or molality and is crucial for applications in medicine, environmental science, and manufacturing. The formation of solutions depends on intermolecular forces, following the principle that "like dissolves like," where polar solvents dissolve polar solutes and nonpolar solvents dissolve nonpolar ones.

Definition and Fundamentals

Core Definition

In chemistry, a solution is a homogeneous mixture of two or more substances where, for convenience, one (or more) substance, designated the solvent, is treated differently from the others, designated the solutes. Solutions can exist in gaseous, liquid, or solid states, though the IUPAC Gold Book specifies liquid or solid phases. This formulation emphasizes the role of the solvent as the primary component in which the solutes are dispersed, forming a unified single-phase system rather than distinct phases. Solutions are homogeneous mixtures, meaning they exhibit uniform composition and properties throughout at the molecular or ionic level, distinguishing them from heterogeneous mixtures such as suspensions or colloids where components are unevenly distributed and visible separation occurs. Unlike pure substances, which consist of a single chemical component with fixed composition, solutions involve multiple substances intermixed without between them, resulting in a single phase that cannot be separated by mechanical means like . A classic example is saltwater, formed when (NaCl) dissolves in : the ionic salt lattice breaks apart as molecules, acting as a polar solvent, surround and separate the Na⁺ and Cl⁻ ions through ion-dipole interactions, yielding a clear, uniform liquid where the ions are fully dispersed at the molecular level. This process exemplifies how solute-solvent interactions drive the formation of solutions, ensuring homogeneity essential for their stability and behavior.

Solvent and Solute Distinction

In a solution, the is defined as the component present in the greater amount, which determines the physical state or of the resulting . For instance, serves as a universal solvent owing to its high , enabling it to dissolve a wide array of substances through strong intermolecular attractions. The solute, in contrast, is the minor component that dissolves into the solvent, and it may exist in , liquid, or gaseous form prior to dissolution. Common examples include () as a solute dissolving in to form a sweetened , or gas as a solute in , creating carbonated beverages. The compatibility between solvents and solutes is governed by the principle "like dissolves like," which posits that polar solvents effectively dissolve polar solutes, while nonpolar solvents dissolve nonpolar solutes. This arises from favorable intermolecular interactions: in polar systems, such as dissolving ionic salts or polar molecules like , dipole-dipole forces and hydrogen bonding stabilize the solute-solvent interface by aligning partial charges. Conversely, nonpolar solvents like dissolve nonpolar substances such as oils through weaker London dispersion forces, as both lack significant charge separation. The distinction between solvent and solute can blur in mixtures where components are present in equal amounts (e.g., 50% mixtures), rendering the conventional major-minor arbitrary and leading to symmetric thermodynamic treatment of the components.

Classification of Solutions

By Physical State

Solutions are classified by the physical states of the solute and solvent, resulting in nine possible combinations, though not all are equally common or stable. This highlights how solutions form across different phases while maintaining homogeneity through molecular-level .

Gaseous Solutions

Gaseous solutions occur when both solute and solvent are gases, such as air, where oxygen acts as the solute dissolved in nitrogen as the solvent through diffusion, forming a homogeneous mixture at the molecular level. Another example is natural gas, primarily methane with minor gaseous impurities like ethane. Gas-in-gas solutions are typically ideal and follow Dalton's law of partial pressures due to the high mobility of gas molecules. Gas-in-liquid solutions involve a gas solute dissolving in a , often requiring to achieve significant , as seen in carbonated beverages like , where is dissolved in under to create upon release. These solutions exhibit behavior at low concentrations, where is proportional to .

Liquid Solutions

Liquid-in-liquid solutions form when two liquids mix, such as dissolved in to produce alcoholic beverages, where the components are miscible due to similar intermolecular forces like hydrogen bonding. , a of hydrocarbons like in , represents non-polar liquid-in-liquid solutions. These mixtures can be ideal or show deviations based on molecular interactions. Solid-in-liquid solutions are among the most familiar, with a solid solute dissolving in a liquid solvent, exemplified by , where (NaCl) dissolves in through ion-dipole interactions, forming hydrated ions. Other examples include in or in , where depends on factors like .

Solid Solutions

Solid-in-solid solutions, also known as alloys, involve a solid solute incorporated into a solid solvent lattice, such as , where atoms substitute for some atoms in the , enhancing properties like malleability. , with in silver, is another example; these interstitial or substitutional alloys maintain a single crystalline phase. Liquid-in-solid solutions feature a liquid solute trapped within a solid solvent, as in hydrated salts like copper(II) sulfate pentahydrate (CuSO₄·5H₂O), where water molecules are incorporated into the ionic crystal lattice, stabilizing the structure through coordination bonds. Gas-in-solid solutions occur when a gas solute is absorbed into a solid , often involving or placement in the . A classic example is gas dissolved in metal, forming (PdH_x), which is used in and purification due to the reversible absorption of atoms into the metal .
TypeSolute StateSolvent StateExample
Gas-in-GasGasGasAir (O₂ in N₂)
Gas-in-LiquidGasLiquidSoda (CO₂ in H₂O)
Liquid-in-LiquidLiquidLiquid in
Solid-in-LiquidSolidLiquid (NaCl in H₂O)
Solid-in-SolidSolidSolid (Zn in Cu)
Liquid-in-SolidLiquidSolidHydrated CuSO₄·5H₂O
Gas-in-SolidGasSolidH₂ in
Regardless of the physical states involved, all solutions are homogeneous, forming a single where solute particles are dispersed at the molecular or ionic level, preventing settling or . In gaseous and liquid solutions, this uniformity is maintained by , the random movement of particles due to collisions with molecules, ensuring continuous mixing. Solid solutions achieve homogeneity through atomic substitution or interstitial placement in the , without the dynamic motion seen in fluid phases.

By Solubility Characteristics

Solutions are classified by their solubility characteristics, which describe the extent to which solutes dissolve in solvents and the resulting states. This classification highlights behavioral traits such as complete mixing, limited , and degrees of , distinguishing solutions based on molecular interactions and stability under given conditions. Miscible solutions form when two or more liquids mix completely in all proportions to produce a homogeneous , as seen in and , where similar polar intermolecular forces, such as hydrogen bonding, allow for uniform dispersion. In contrast, immiscible solutions, like oil and , do not mix fully and separate into distinct layers due to differing intermolecular forces—nonpolar van der Waals interactions in oil versus polar hydrogen bonding in —resulting in minimal mutual . These distinctions arise from the relative strengths of attractive forces between like and unlike molecules, determining whether a single phase or multiple phases form upon mixing. Saturated solutions represent a state of at a specific and , where the maximum amount of solute has dissolved, and no additional solute can dissolve without altering conditions; here, the rate of solute equals the rate of , maintaining a constant solute concentration. This is reversible, with solute particles continuously dissolving and recrystallizing at matching speeds. Unsaturated solutions contain less solute than the saturation limit for the given conditions, allowing further dissolution without precipitation. Supersaturated solutions, however, hold more solute than permits, often achieved by cooling a hot saturated solution or through , rendering them metastable and highly unstable; introducing a or agitation triggers rapid , as exemplified in solutions used in reusable hand warmers, where the excess solute precipitates exothermically upon . Partial occurs in systems where liquids are soluble only within limited concentration ranges, forming two coexisting phases, and this behavior is often -dependent. For instance, the nicotine-water system exhibits complete miscibility at but shows decreasing mutual as rises, with an upper consolute temperature around 208°C above which the liquids become fully immiscible, and a lower consolute temperature near 61°C below which they mix completely; this reflects changes in intermolecular interactions with .

Key Physical Properties

Solubility Factors

The solubility of a solute in a is fundamentally governed by the change in (\Delta G) for the process, where \Delta G = \Delta H - T \Delta S; for to occur spontaneously, \Delta G must be negative, with the positive (\Delta S > 0) often driving by increasing disorder in the solution. The change (\Delta H) reflects the energy required to break solute-solute and solvent-solvent interactions versus forming solute-solvent bonds, while (T) modulates the entropy term's influence. Temperature significantly affects solubility, typically increasing it for most solid and liquid solutes due to endothermic dissolution processes where heat absorption favors higher solubility at elevated temperatures—for instance, more sugar dissolves in hot tea than in cold water. In contrast, gas solubility decreases with rising temperature because gas dissolution is generally exothermic, shifting the equilibrium toward the undissolved state as per Le Chatelier's principle. Exceptions exist, such as certain salts like sodium sulfate, where solubility decreases with temperature due to exothermic dissolution. Pressure has negligible impact on the solubility of solids and liquids owing to their low , but it markedly influences gases, as described by : the (S) of a gas is directly proportional to its (P) above the solution, expressed as S = k_H P, where k_H is the Henry's law constant specific to the gas-solvent pair. This relationship explains phenomena like increased dissolution in carbonated beverages under , with decreasing upon opening the container as drops. The chemical of the solute and the solution's play roles, particularly for acidic or solutes; weak acids exhibit greater in conditions because the conjugate ionizes and forms soluble ionic , while weak bases are more soluble in acidic environments due to . For example, the of a weak acid like acetic acid increases in alkaline solutions as it deprotonates to ions, which are highly soluble. The reduces when an ion from the solute is already present in the solution from another source, suppressing dissociation via ; for instance, adding NaCl decreases the of AgCl because the shared Cl⁻ ion shifts the equilibrium leftward, limiting further AgCl dissolution. In a 0.1 M NaCl solution, AgCl drops to approximately 1.8 × 10⁻⁹ M compared to pure water, illustrating this effect quantitatively.

Colligative Properties

Colligative properties are physical characteristics of solutions that depend solely on the concentration of solute particles in terms of their number, rather than their chemical identity or nature. These properties arise from the interactions between solute particles and the solvent, particularly in dilute solutions where ideal behavior is approximated. They are crucial for understanding phenomena such as phase changes and pressure effects in solutions, and their quantitative descriptions are derived under assumptions of ideal mixing and negligible solute-solute interactions. Vapor pressure lowering is one of the fundamental , described by for ideal solutions. According to , the partial of the in a solution, P, is equal to the of the pure , P^\circ, multiplied by the of the , X_{\text{solvent}}:
P = P^\circ \cdot X_{\text{solvent}}
This law is derived from the assumptions of behavior and random mixing, where solute particles occupy sites on the surface, reducing the proportion of molecules that can evaporate. For a binary solution, the total is the sum of the 's partial and the solute's, but in dilute solutions with non-volatile solutes, the solute contribution is negligible, leading to an overall decrease in proportional to the solute concentration.
Boiling point elevation occurs because the lowered of the solution requires a higher to reach compared to the pure . The change in boiling point, \Delta T_b, is given by \Delta T_b = K_b \cdot m, where K_b is the molal boiling point elevation constant specific to the , and m is the of the solute (moles of solute per of ). This relationship holds for non-volatile solutes in dilute solutions and stems from the need to increase the 's to match the external . For example, adding to increases its boiling point, so seawater boils at a higher than pure at the same , a principle used in cooking to shorten boiling times for . Freezing point depression is analogous, where the presence of solute particles disrupts the formation of the pure 's crystal lattice, lowering the temperature at which the solution freezes. The freezing point change is expressed as \Delta T_f = K_f \cdot m, with K_f as the molal freezing point depression constant for the . This effect is particularly useful in applications like , where dissolved in lowers the freezing point to prevent engine damage in cold climates, allowing the mixture to remain below 0°C. Osmotic pressure, another colligative property, is the pressure required to prevent the flow of across a separating the solution from pure , driven by the tendency to equalize concentrations. It is quantified by the van't Hoff equation: \pi = i \cdot [M](/page/M) \cdot [R](/page/R) \cdot T, where \pi is the , i is the van't Hoff factor accounting for the number of particles per solute molecule (e.g., i = 2 for NaCl due to ), M is the molarity, R is the , and T is the absolute temperature. This equation derives from treating solute particles as exerting an effective pressure similar to ideal gases. In biological systems, maintains cell turgor and regulates water movement across membranes, such as in red blood cells where imbalances can lead to . In non-ideal solutions, deviations from these occur due to specific solute- or solute-solute interactions that alter the effective concentration or activity of particles. Positive deviations, where the observed effect is greater than predicted (e.g., larger lowering), arise from weaker interactions than in the pure , as seen in ethanol-water mixtures. Negative deviations, conversely, result from stronger interactions, such as hydrogen bonding in acetone-chloroform solutions, leading to smaller effects than ideal. These deviations are quantified using activity coefficients, but for most practical dilute solutions, the ideal approximations suffice.

Measurement and Concentration

Units of Concentration

Concentration of a solution quantifies the amount of solute dissolved in a given amount of solvent or solution, with various units employed depending on the context and required precision. Common units include , , , mass percentage, and parts per million, each offering distinct advantages for different applications in . Molarity (M), denoted as moles of solute per liter of solution, is a widely used unit for expressing concentration in settings. It is calculated as the of the moles of solute to the volume of the entire solution in liters. However, molarity is temperature-dependent because the volume of the solution expands or contracts with temperature changes, altering the concentration value. Molality (m), defined as the moles of solute per of , provides a temperature-independent measure of concentration, as it relies on rather than . This property makes particularly suitable for calculations involving , where temperature variations must not affect the results. The (X) represents the ratio of the moles of a specific component to the total moles of all components in the solution, resulting in a dimensionless value between 0 and 1. It is especially useful in thermodynamic analyses, such as applying to determine vapor pressures over solutions. For less concentrated solutions, mass-based units like mass percentage and (ppm) are often preferred, particularly in environmental and industrial contexts. Mass percentage is the mass of solute divided by the total mass of the solution, multiplied by 100, providing a straightforward way to describe solution composition. expresses the mass of solute per million parts of solution, equivalent to milligrams of solute per of solution, and is ideal for trace amounts; for instance, the average of is about 35,000 ppm, reflecting the total dissolved salts. Converting between units, such as from to , requires knowledge of the solution's to account for the mass relationships. For a 1 M NaCl solution with a density of 1.037 g/mL at 25°C, the mass of 1 L of solution is 1037 g. The mass of NaCl in this volume is 58.44 g (1 mol × 58.44 g/mol), so the mass of solvent () is 1037 g - 58.44 g = 978.56 g ≈ 0.979 kg. Thus, the is 1 mol / 0.979 kg ≈ 1.02 m. These units, particularly and , find application in colligative property formulas.

Ideal vs. Non-Ideal Solutions

Ideal solutions are homogeneous mixtures that obey over the entire range of compositions, where the partial vapor pressure of each component is equal to its multiplied by the of the pure component. In such solutions, the (ΔH_mixing) and volume of mixing (ΔV_mixing) are both zero, indicating no net heat absorption or release upon mixing and no change in volume, due to equivalent intermolecular interactions between like and unlike molecules. A classic example is the benzene-toluene system, where the components have similar molecular structures and interactions, leading to behavior closely approximating ideality. Non-ideal solutions deviate from , exhibiting either positive or negative deviations based on intermolecular forces. Positive deviations occur when solute-solvent interactions are weaker than those in the pure components, resulting in higher-than-expected total and thus lower points compared to ideal predictions. For instance, the ethanol-water mixture shows positive deviation due to disrupted hydrogen bonding in the pure liquids upon mixing, forming a minimum-boiling azeotrope at approximately 95.6% by weight, which boils at 78.2°C. In contrast, negative deviations arise from stronger solute-solvent interactions than in the pure states, leading to lower total and higher boiling points. The -acetone system exemplifies this, where hydrogen bonding between and acetone enhances stability, producing a maximum-boiling azeotrope at about 64% by , boiling at 64.5°C. To account for non-ideality, activity coefficients (γ) are introduced, where the effective concentration (activity) of a component is γ times its ; for ideal solutions, γ = 1 for all components. These deviations are often visualized in temperature-composition () phase diagrams, where positive deviations show a minimum in the curve and negative deviations a maximum, highlighting the azeotropic points. Azeotropes pose significant challenges in distillation, as they cannot be further separated by simple since the vapor and liquid compositions become identical at the azeotropic point, limiting purity achievable through conventional methods. This necessitates alternative techniques like azeotropic or for systems such as ethanol-water.

Preparation and Stability

Methods of Preparation

Solutions are commonly prepared by , where a measured amount of solute is added to a and mixed until fully dissolved. This method is efficient for creating solutions of known concentration, such as solutions, by first weighing the precise mass of solid solute using an and then gradually adding it to the while stirring to promote uniform dispersion. Heating the can accelerate the process for solutes with limited at , but care must be taken to avoid or excessive . Dilution involves adding a measured of concentrated stock solution to to achieve the desired concentration in the final , ensuring efficient use of materials and minimizing handling of large volumes of solute. The volume-based calculation follows the C_1 V_1 = C_2 V_2, where C_1 and V_1 are the concentration and of the stock solution, and C_2 and V_2 are the target concentration and final , respectively; this allows precise preparation by measuring and combining appropriate volumes. To maintain accuracy, the stock solution is added to the rather than vice versa, and the is thoroughly mixed to ensure homogeneity. In some cases, solutions are prepared through chemical reactions that generate the solute , such as acid-base neutralization to form solutions. For example, reacting with produces a solution via the neutralization process, where the ions combine to yield the dissolved directly in the aqueous medium. This method is particularly useful for creating solutions of compounds that are unstable or difficult to handle as pure solids. Specialized techniques enhance preparation efficiency for challenging solutes. Ultrasonication employs high-frequency sound waves to disperse poorly soluble particles, such as nanoparticles, by generating bubbles that break aggregates and improve homogeneity in the solution. For gases, high-pressure methods increase according to principles like applications, where elevated pressure forces more gas molecules into the liquid phase, as seen in carbonated beverage production. Safety is paramount in solution preparation to prevent hazards from exothermic reactions or volatile components. Dissolving in releases significant heat, potentially causing splattering or burns, so the solid should be added slowly to excess in a while using protective equipment. Similarly, preparing solutions with volatile solvents requires adequate , such as a , to avoid of vapors and ensure efficient dispersal of any released gases.

Saturation and Supersaturation

A saturated solution is one in which the concentration of the dissolved solute has reached the maximum possible value at a given temperature and pressure, preventing further dissolution of additional solute. Saturation can be detected qualitatively by adding excess solute; if the added material remains undissolved and settles or causes cloudiness upon stirring, the solution is saturated. Quantitatively, saturation is assessed using solubility curves, which graphically represent the maximum solute concentration as a function of temperature, allowing comparison of the solution's concentration to the equilibrium limit. Supersaturation occurs when a solution holds more solute than the saturation limit at a specific , typically achieved through methods such as gradual cooling of a hot saturated solution or without initiating , resulting in a metastable state prone to sudden . In this metastable condition, the excess solute remains dissolved until a disrupts the , leading to rapid . Crystallization from supersaturated solutions is triggered by , which can be induced through with a small crystal fragment to provide a surface for growth, scratching the container walls to create microscopic defects as nucleation sites, or mechanical agitation to promote solute clustering. A classic example is formation, where a supersaturated solution is prepared by dissolving in hot and cooling it slowly; a string or stick dipped in the solution serves as a heterogeneous nucleation site, allowing large crystals to grow over time. The stability of supersaturated solutions is influenced by factors such as and impurities; finer solute particles can lower the energy barrier for due to increased surface area, while impurities act as heterogeneous sites that reduce the and promote earlier . In laboratory settings, is controlled by adjusting cooling rates during preparation; slow cooling maintains higher levels by minimizing thermal gradients that could trigger spontaneous , whereas faster cooling achieves more rapidly but risks uncontrolled , enabling precise management for experimental reproducibility.

Applications and Modern Developments

Traditional Uses

Solutions have played a pivotal role in human activities since , evolving from pursuits to foundational applications in modern chemistry. In , practitioners utilized various solutions, including aqueous mixtures of metals, salts, and acids, to pursue of metals into and the creation of life-prolonging elixirs, laying early groundwork for chemical experimentation. By the , these traditions transitioned into practical chemistry, notably in , where solutions of silver halides—such as and —were essential for creating light-sensitive emulsions in processes like daguerreotypes and wet plates. In industrial settings, solutions form the basis of numerous traditional processes. Aqueous solutions of detergents, developed from early 20th-century synthetic , serve as effective cleaning agents by emulsifying oils and greases in laundry, dishwashing, and surface preparation, replacing traditional soaps in environments. , pioneered in 1805 by Luigi Brugnatelli using a , employs electrolyte solutions of metal salts—like or nickel sulfate—to deposit protective or decorative metal coatings on objects, enhancing durability in applications from to machinery parts. Medically, solutions enable precise delivery of essential substances. Intravenous saline, a 0.9% solution in , has been a staple since the late for restoring fluid and balance in patients experiencing , , or , mimicking the body's to avoid cellular damage. Oral liquid medications, formulated as aqueous or hydroalcoholic solutions of active pharmaceutical ingredients, facilitate dosage control and for patients unable to solids, such as children or the elderly. In daily life, solutions are ubiquitous in and . Sugar syrups, concentrated aqueous solutions of (often 60-70% by weight), provide sweetness and preservation in beverages like sodas, teas, and cordials, dissolving readily to ensure uniform flavor distribution. In , foliar fertilizer sprays apply dilute aqueous solutions of nutrients—such as from or from phosphates—directly to plant leaves, promoting growth in nutrient-deficient soils without relying solely on root uptake. Environmentally, natural solutions influence global systems. , an solution containing about 3.5% dissolved salts like , exhibits variations due to gradients; higher increases , causing to sink and drive , which powers ocean currents and distributes heat worldwide.

Recent Advancements

In recent years, advancements in have significantly enhanced the role of solutions in , particularly for targeted cancer therapies. Liposomal , which form stable colloidal solutions in aqueous media, have been engineered to encapsulate chemotherapeutic agents, improving and reducing systemic . For instance, loaded with gene-editing components or small molecules have shown promise in treatment, with stability maintained through coatings that prevent aggregation and enhance cellular uptake. Between 2020 and 2025, these systems have enabled inhalable formulations for localized delivery, achieving higher therapeutic indices in preclinical models. Green chemistry principles have driven the adoption of ionic liquids as sustainable solvents in biomass processing, offering alternatives to volatile organic compounds that minimize environmental impact. These room-temperature ionic liquids facilitate the dissolution and fractionation of lignocellulosic biomass, enabling efficient depolymerization into value-added chemicals while reducing waste generation through recyclability. Silica-supported imidazolium-based ionic liquids, for example, have demonstrated high catalytic activity in biomass valorization, achieving near-complete conversion with minimal energy input and solvent loss in cyclic processes. Such developments, prominent since 2020, support biorefinery operations by lowering operational costs and emissions compared to traditional methods. In , solution-processed inks have revolutionized thin-film fabrication for solar cells, yielding efficiencies exceeding 25% by 2025. These halide solutions, deposited via scalable techniques like blade coating, form high-quality polycrystalline films with minimal defects, enhancing charge transport and . As of November 2025, tandem -silicon devices have reached certified efficiencies of 33.6% for flexible configurations, attributed to optimized precursor compositions that mitigate phase segregation under operational . All- tandems have achieved 31.38% efficiency as of 2025, with open-circuit voltages up to 2.21 V, paving the way for cost-effective photovoltaic . Biotechnological innovations include optimized buffer solutions for CRISPR-Cas9 gene editing, which improve editing precision and efficiency in therapeutic applications. Tailored buffers incorporating specific salts and pH modifiers have enhanced ribonucleoprotein complex stability, boosting on-target indel formation rates by up to 50% in mammalian cells. These formulations, refined through high-throughput screening since 2020, reduce off-target effects and support in vivo delivery via lipid nanoparticles for diseases like hemophilia. The have seen AI-driven predictions of parameters accelerate pharmaceutical , enabling of vast compound libraries to identify candidates with optimal aqueous . models, trained on structural data, have predicted with root-mean-square errors below 0.5 log units, shortening development timelines from years to months. In parallel, carbon capture technologies have advanced with catalyst-enhanced amine-based absorbents; for example, pyrophosphate catalysts integrated into monoethanolamine solutions have improved CO2 desorption by reducing relative heat duty by 13% in 2024 laboratory studies, facilitating scalable .

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