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Multituberculata

Multituberculata is an extinct order of rodent-like mammals belonging to the infraclass , distinguished by their specialized featuring multiple rows of low cusps (tubercles) on the upper and lower molars, which facilitated efficient grinding of plant material and possibly other foods. These mammals ranged in size from small, mouse-like forms to larger species comparable to beavers, and they exhibited diverse adaptations including burrowing, climbing, and terrestrial locomotion. Originating in the around 170–175 million years ago, multituberculates achieved their greatest diversity during the and early , with over 200 species documented across all continents except and . They survived the mass extinction event approximately 66 million years ago but gradually declined in diversity through the , ultimately becoming extinct by the late Eocene around 35 million years ago. Phylogenetically positioned as a stem group outside the mammals (placentals and marsupials), multituberculates are notable for their prolonged evolutionary history exceeding 130 million years, the longest of any mammalian order, and evidence from bone histology suggests they employed a life history akin to that of placental mammals, involving extended rather than the short pregnancies typical of marsupials. Their decline has been attributed to competitive exclusion by emerging , which possessed superior bite forces and adaptive versatility in exploiting changing environments, including the spread of angiosperm-dominated floras. Despite their extinction, multituberculates highlight the ecological success and morphological innovation of early s during the era, filling niches later dominated by and lagomorphs.

Anatomy and Morphology

Dentition and Skull

Multituberculates possessed a highly specialized characterized by enlarged, ever-growing incisors, a prominent separating the incisors from the cheek teeth, and distinctive molars and adapted for both shearing and grinding. The cheek teeth featured multiple longitudinal rows of cusps, typically two or three, arranged on the surfaces of the upper and lower molars and , enabling efficient pulverization of food through precise cusp-to-cusp . A defining feature was the enlarged fourth lower , known as the plagiaulacoid, which formed a blade-like structure with serrated edges and multiple cusps, functioning primarily for shearing tough plant material or possibly small . This supported a palinal (posteriorly directed) jaw stroke during mastication, where the lower teeth moved backward relative to the uppers, contrasting with the propalinal motion seen in . The of multituberculates exhibited an elongated, rodent-like rostrum that housed the procumbent incisors and contributed to the of feeding. A substantial between the incisors and the first allowed space for jaw movement without interference, while the zygomatic arches were robust to accommodate powerful masseter muscles. The often lacked vacuities in more primitive forms but showed variations, and the was relatively narrow with a prominent forming much of the lateral wall. Recent high-resolution scans of specimens, such as the kogaionid Litovoi tholocephalos, reveal an with intermediate complexity: a narrow overall shape reminiscent of early mammals, yet incorporating therian-like features such as expanded paraflocculi and a relatively large , bridging primitive and derived mammalian . Dentition varied significantly across multituberculate groups, reflecting evolutionary progression from primitive to advanced forms. In the basal Plagiaulacida, the plagiaulacoid was fully developed and blade-like, with premolars and molars bearing simpler, more triangular cusps suited to an omnivorous or insectivorous diet, and low-crowned (brachyodont) teeth overall. In contrast, the more derived Cimolodonta, dominant from the onward, showed reduction or modification of the plagiaulacoid, with molars evolving toward hypsodonty—taller crowns with increased occlusal wear surfaces—in later taxa, enhancing adaptation to abrasive herbaceous diets. These shifts paralleled broader cranial refinements, such as a more robust rostrum and enhanced jaw musculature in cimolodontans.

Postcranial Features

Multituberculates displayed considerable variation in body size, ranging from diminutive, shrew-like forms to large, beaver-sized individuals, reflecting their adaptation to diverse ecological niches across the and eras. The earliest known multituberculate, Rugosodon eurasiaticus from the Middle Jurassic of , represents one of the smallest, with a total body length of approximately 20 cm and an estimated mass under 100 g, comparable to modern . In contrast, the taeniolabidoid Taeniolabis taoensis from achieved the largest size among all multituberculates, with estimated body masses of up to approximately 40 kg based on cranial measurements, akin to a large , and postcranial elements indicating a robust build consistent with this size. This size disparity, spanning over three orders of magnitude, underscores the group's evolutionary flexibility in response to environmental changes. Limb morphology in multituberculates was diverse, supporting a range of locomotor strategies from terrestrial cursoriality to potential scansorial or saltatorial behaviors. In taxa like the Kryptobaatar dashzevegi from , the forelimbs were robust with abducted humeri and strong muscular attachments, suggesting adaptations for digging or burrowing activities, as evidenced by the twisted humeral shaft and large deltopectoral crest for powerful forelimb retraction. Hindlimbs in many forms, such as the Ptilodus gracilis, featured relatively elongated femora and tibiae with a large and pronounced peroneal process on the , indicating saltatorial capabilities for leaping or possibly arboreal climbing, though phalangeal robusticity points more toward generalized terrestrial locomotion than specialized arborealism. Overall, limb proportions reflect a predominantly sprawling with abducted limbs, but with parasagittal elements in the for efficient forward propulsion during asymmetrical gaits. Vertebral and pelvic structures provided support for dynamic movement and reproductive strategies. The in Asian multituberculates like Kryptobaatar included long, craniodorsally sloping spinous processes, facilitating an asymmetrical with short jumps and enhanced spinal flexibility for maneuvering. Pelvic adaptations, such as a deep with a large iliosacral angle of 35–37° and dorsoventral contact between ilium and , suggest stability for weight-bearing during locomotion and potential for live birth, as the broad pelvic canal accommodated passage of young without the narrow constraints seen in monotremes. Recent bone analyses further support a placental-like reproductive strategy with extended , implying live birth to relatively developed rather than marsupial-style pouch rearing. In Taeniolabis, the robust and short caudal vertebrae indicate a stable base for its large body mass, with elements in the hinting at digging behaviors. Fossil evidence for soft tissues includes and fur impressions, offering insights into sensory and thermoregulatory functions. Caudal vertebrae in forms like Catopsbaatar catopsaloides were numerous and elongated, suggesting a long, flexible possibly used for balance during jumping or as a sensory organ, similar to modern . impressions preserved in coprolites of the Eocene Lambdopsalis bulla reveal a dense pelage with guard hairs and underfur, indicating and protection against environmental stresses, with no evidence for prehensile capabilities but potential for insulation in varied habitats. These features align with the group's inferred physiology and active lifestyles.

Taxonomy and Phylogeny

Historical Classification

The first recognized multituberculate fossils were teeth from the Purbeck Group of , described as the genus Plagiaulax by Hugh Falconer in 1857. Falconer interpreted these specimens as belonging to an extinct herbivorous , based on their multicusped premolars and overall dental morphology. This initial classification reflected the limited understanding of mammals at the time, with the unusual tuberculate teeth leading to comparisons with both reptilian and marsupial forms rather than recognizing their distinct mammalian affinities. Subsequent work by in 1871 expanded on these discoveries through a monograph detailing mammals from the collections, including additional Plagiaulax material and new genera like Bolodon. Owen confirmed their mammalian nature but emphasized their aberrant , placing them in a provisional group separate from typical therian mammals and suggesting possible links to monotremes due to shared primitive features. In the late 19th century, advanced the taxonomy by naming numerous North American species, such as Taeniolabis in 1882, and formally establishing the order Multituberculata in 1884 as a suborder within Marsupialia, highlighting similarities in their specialized cheek teeth to those of . Cope's framework sparked ongoing debates about multituberculate affinities, with some researchers, including in his studies of early 20th-century faunas, proposing closer ties to based on ecological and dental parallels. George Gaylord Simpson's 1928 catalogue of mammals synthesized these early findings and provided the first comprehensive classification, dividing Multituberculata into three suborders: the primitive Plagiaulacida (encompassing and forms like Plagiaulax), and the more derived Ptilodontoidea and Taeniolabidoidea (dominating and assemblages). Simpson's scheme underscored their evolutionary distinctiveness while maintaining debates on rodent-like adaptations, such as grinding suited to herbivory. By the mid-20th century, Zofia Kielan-Jaworowska's expeditions to the in the 1960s and 1970s uncovered exceptionally preserved specimens, including skulls and postcrania, which demonstrated unique anatomical features like inflected angular processes and specialized jaw mechanics, solidifying Multituberculata's status as a separate mammalian order rather than a or offshoot. These contributions culminated in the 1970s with the broader recognition of Multituberculata within the subclass , a grouping initially proposed by Cope but refined to encompass their basal position relative to other mammals, based on shared dental and cranial traits with extinct haramiyidans. This historical progression from misclassification as marsupials or reptiles to an independent laid the groundwork for understanding their long evolutionary history.

Modern Suborders and Families

The modern taxonomic framework for Multituberculata recognizes two primary suborders based on dental morphology, postcranial features, and stratigraphic distribution, as outlined in the comprehensive revision by Kielan-Jaworowska, Cifelli, and Luo (2001). The suborder Plagiaulacida is considered paraphyletic, representing a grade of early, primitive forms that bridge the basal multituberculates to more derived lineages, while Cimolodonta forms a monophyletic clade defined by shared apomorphies such as advanced occlusal patterns in the lower premolars. This classification has remained influential, though recent phylogenetic studies using tip-dating methods have refined relationships among early allotherians, rejecting a strict monophyly of Allotheria and suggesting some haramiyidan-like forms may nest within multituberculate diversity, with Gondwanatheria positioned outside the group. Plagiaulacida spans the to and is characterized by simpler tuberculate suited to insectivory or omnivory, with fossils primarily from Laurasian continents. Key families include Plagiaulacidae (e.g., Plagiaulax from the of ), Albionbaataridae (e.g., Albionbaatar from the of ), and Paulchoffatiidae (e.g., Paulchoffatia from the of ). The earliest definitive multituberculates date to the Bathonian stage, such as Hahnotherium from . An important early multituberculate, Rugosodon eurasiaticus, from the of , exemplifies early dental specializations like multiple premolars. These taxa exhibit low diversity, with fewer than 50 described species, reflecting their role as stem-group forms before the cimolodontan radiation. Cimolodonta, the dominant suborder, ranges from the to the late Eocene and achieved peak diversity in the and of and , with over 150 species described. This suborder is divided into several superfamilies, including Ptilodontoidea (e.g., families Ptilodontidae with Ptilodus from the of , known for its rodent-like grinding ) and Taeniolabidoidea (e.g., Taeniolabididae with Taeniolabis, the largest known multituberculate at up to 50 kg body mass from the of ). Other notable families include Djadochtatheriidae (e.g., Kryptobaatar from the of , renowned for well-preserved skeletons showing adaptations) and Eucosmodontidae (e.g., Eucosmodon from the of ). Cimolodontans occupied diverse niches, from arboreal to terrestrial, across , with isolated records in and . The following table summarizes major families within each suborder, highlighting temporal and geographic distributions:
SuborderFamilyTemporal RangeGeographic RangeKey Genera Examples
PlagiaulacidaPlagiaulacidaePlagiaulax, Bolodon
PlagiaulacidaAlbionbaataridaeAlbionbaatar
PlagiaulacidaPaulchoffatiidaePaulchoffatia
CimolodontaPtilodontidae–EocenePtilodus, Baiotomeus
CimolodontaTaeniolabididaePaleocene–EoceneTaeniolabis, Catopsalis
CimolodontaDjadochtatheriidaeKryptobaatar, Nemegtbaatar
Overall, as of 2025, over 200 species of multituberculates have been described, with recent additions including Novaculadon mirabilis from the of and Cambelodon torreensis from the of , underscoring their status as the most diverse Mesozoic mammalian order, though undescribed material from ongoing excavations in and suggests higher true diversity.

Phylogenetic Relationships

Multituberculata are classified within the , where they form the to Euharamiyida based on shared dental features such as multicusped upper molars and specialized lower premolars. This relationship is supported by phylogenetic analyses of specimens, which demonstrate that diverged early from other , potentially within or basal to crown Mammalia. Recent studies, including tip-dating approaches, have challenged the of by separating multituberculates from certain haramiyidans, positioning the former closer to therians while placing some haramiyidans outside . The affinities of Multituberculata have long been debated, with historical proposals linking them to monotremes due to primitive cranial features or to basal therians based on mechanics, though cladistic now favors an independent allotherian lineage outside the monotreme-therian split. Similarities in to , such as molars adapted for grinding, are recognized as convergent adaptations rather than indicating close relationship, as multituberculates lack rodent-specific traits like ever-growing incisors. Inclusion of within Multituberculata or has been rejected in multiple analyses due to differences in postcranial and microstructure, supporting as a distinct allied with euharamiyidans instead. Cladograms from 2020s phylogenetic studies highlight key synapomorphies defining Multituberculata, including multituberculate molars with two or more transverse rows of low cusps for crushing and a enlarged, blade-like plagiaulacoid fourth lower for shearing tough material. These trees, often derived from Bayesian tip-dating of dental and cranial matrices, consistently recover origins for the group, branching from a common ancestor shared with other early mammaliaforms around the . Comparisons with outgroups such as , an early mammaliaform, underscore evolutionary contrasts in and structures: retained a primitive quadrate-articular and multiple postdentary bones, whereas multituberculates evolved a mammalian-style dentary-squamosal alongside a partially detached featuring an inflated petrosal and reduced angular process. In contrast to therians, which exhibit a fully derived three-ossicle system with complete separation of hearing and mastication elements, multituberculates show an intermediate condition with independent of ear detachment, reflecting mosaic adaptations in allotherians.

Evolutionary History

Origins in the Jurassic

The earliest known multituberculates appeared during the , approximately 168 million years ago, with fossil evidence from the stage in both and . In , isolated teeth attributed to Hahnotherium antiquum represent the oldest unequivocal records from the Forest Marble Formation of and Dorset, showcasing primitive dental features such as low lingual cusps on molars indicative of an early stage in multituberculate evolution. Similarly, in western , , teeth from the Itat Formation at the Berezovsk coal mine belong to new genera like Tashtykia primus and Tagaria antiqua, confirming a contemporaneous presence in Laurasian landmasses and suggesting an initial dispersal across northern continents during this period. These primitive forms were small-bodied mammals, with estimated body masses under 100 grams, comparable to modern or small , and exhibited suited to an insectivorous or omnivorous diet including , seeds, and soft plant matter. For instance, the more complete Late Jurassic skeleton of Rugosodon ostromi from China's Yanliao Biota, dated to around 160 million years ago, reveals versatile molars with multiple cusps for crushing and grinding, alongside evidence of a flexible ankle supporting scansorial habits— and arboreal activities in trees or shrubs. Such traits align with the basal position of multituberculates within , potentially evolving from haramiyidan ancestors, as supported by recent analyses of dental morphology in Middle Jurassic specimens that bridge simple cusp patterns to the specialized multituberculate structure. The initial radiation of multituberculates during the was modest, encompassing roughly 10 genera across , with fossils primarily from forested or riparian environments that also hosted early dinosaurs such as basal theropods and ornithischians. This limited diversity reflects an adaptive phase in humid, vegetated habitats like the lagoonal settings of the Forest Marble or the coal-bearing swamps of the Itat Formation, where multituberculates likely occupied insectivorous niches amid a burgeoning ecosystem.

Cretaceous and Paleogene Diversification

During the , multituberculates underwent a significant , particularly among the cimolodontans, which shifted toward herbivory and plant-dominated omnivory as indicated by increasing occlusal complexity in their cheek teeth. This period saw generic richness rise to approximately 21 genera between 84 and 66 million years ago, with dozens more known overall from n and n faunas, reflecting a boom in diversity driven by ecological opportunities from angiosperm expansion. In , genera such as Meniscoessus exemplified this diversification, occupying niches as medium-sized herbivores in floodplains and coastal environments. Similarly, in , Kryptobaatar from Mongolian formations like the Djadochta represented robust, specialized forms adapted to arid habitats. Recent discoveries, including a new multituberculate species from the (Berriasian) of Dorset, , further illuminate the early stages of this radiation. Following the Cretaceous-Paleogene (K-Pg) mass extinction event around 66 million years ago, multituberculates demonstrated remarkable survivorship, with lineages persisting and radiating further in the . In the early (Puercan and Torrejonian stages), they achieved peak diversity, often comprising over 50% of mammalian species in North American faunas and dominating local assemblages in some sites. This post-extinction success likely stemmed from adaptations such as ground-dwelling or semi-fossorial habits, potentially including burrowing behaviors that buffered against environmental perturbations like wildfires and climate shifts during the extinction aftermath. By the Eocene, forms like Neoliotomus persisted in North American woodlands, showcasing continued niche occupation amid increasing competition from . Geographically, multituberculates remained predominantly Laurasian, with abundant records from , , and , but rare occurrences in southern continents such as isolated finds in and , including recent multituberculate remains from the in , , highlighting limited Gondwanan dispersal. Body size notably increased during this interval, with taeniolabidids like Taeniolabis exceeding 1 kg—some reaching up to 40 kg (averaging around 20-25 kg)—enabling exploitation of larger herbivorous roles in ecosystems. Recent isotopic analyses, including carbon isotope studies from 2023, further elucidate dietary shifts, revealing that elevated δ¹³C values in multituberculate enamel reflect broader environmental influences like atmospheric CO₂ changes rather than unique physiological traits, supporting a transition to C3-plant based diets across vertebrates.

Late Decline Patterns

The diversity of multituberculates underwent a marked reduction beginning in the mid-, with Torrejonian (early to middle Paleocene) faunas showing high taxonomic richness that declined into the Tiffanian (late Paleocene), where species richness reached a low point in the middle Tiffanian before a partial rebound. This faunal turnover marked a shift from around 50 genera documented across and early Paleocene assemblages to fewer than 20 genera persisting into the , reflecting a progressive loss of lineages in North American records. By the Eocene, multituberculate survivors were largely confined to western , where genera such as Ectypodus (Neoplagiaulacidae) persisted, adapting to evolving floral communities in forested habitats. These isolated populations, including species like Ectypodus tardus, represent the final holdouts, with no new genera emerging to offset earlier losses. Regionally, multituberculates vanished from by the late and from by the early Eocene, contributing to the overall contraction of their global range. Quantitative analyses of fossil records reveal trends consistent with , as species-area curves from Paleogene deposits indicate reduced diversity correlating with smaller, isolated depositional basins in western during the Eocene. These patterns underscore a gradual diminishment rather than abrupt loss, contrasting with the peak diversity achieved in the preceding and early phases.

Paleoecology and

Diet and Niche Occupation

Multituberculates exhibited a broad dietary spectrum that evolved over their long history, transitioning from primarily insectivorous or omnivorous habits in early forms to more specialized herbivory in later lineages. multituberculates, such as Rugosodon eurasiaticus, possessed dentitions suited for crushing and grinding small , , and possibly soft matter, indicating an opportunistic omnivorous diet that included and early angiosperm fruits. By the and , particularly within the Cimolodonta suborder, many species shifted toward folivory and granivory, with complex molar occlusal surfaces adapted for shearing and pulverizing tough vegetation. Dental microwear patterns in cimolodontans reveal striations and pits consistent with the ingestion of abrasive plant materials, such as phytoliths and grit from foliage and , supporting a predominantly herbivorous in these advanced forms. Ecological niche occupation among multituberculates was characterized by adaptations that facilitated partitioning from contemporaneous mammals, reducing direct competition for resources. Their unique palinal jaw stroke and multi-cusped molars enabled efficient transverse grinding of fibrous plants, a mechanism less common in early s and allowing multituberculates to exploit tough, abrasive vegetation that s avoided. For instance, the ptilodontoid Ptilodus is interpreted as a specialized predator and granivore, capable of husking and cracking hard-shelled s with its robust premolars and molars, a niche that minimized overlap with browsing or frugivorous s. This specialization likely contributed to their diversification in forested environments, where they filled roles as primary consumers of s and leaves, distinct from the more carnivorous or soft-fruit diets of many coexisting marsupials and placentals. Evidence of scavenging behavior further highlights multituberculates' opportunistic niche, with tooth marks attributed to them found on bones of larger vertebrates, including a Champsosaurus femur. These parallel grooves, matching the paired incisors of multituberculates, indicate gnawing on for or minerals, suggesting they supplemented their plant-based diets with animal remains in carrion-rich ecosystems. Such habits underscore their vulnerability as small-bodied mammals in predator-dominated landscapes, where access to carcasses could expose them to risks from larger carnivores, yet also demonstrate ecological flexibility in occupying detritivore-like roles.

Locomotion and Habitats

Multituberculates displayed considerable locomotor diversity, adapting to fossorial, arboreal, and terrestrial cursorial lifestyles based on postcranial skeletal features analyzed through multivariate morphometric methods. Fossorial adaptations, evident in genera like Fruitafossor from the Early Cretaceous Jehol Biota, included robust postcranial elements such as enlarged scapulae, prominent humeral deltopectoral crests, large olecranon processes, and hypertrophied manual phalanges, which facilitated powerful digging and head-lift burrowing motions. Arboreal forms, such as Ectypodus from the Paleocene and Eocene of North America, featured gracile limbs with slender humeri, small olecranon processes, elongate phalanges, and tarsal specializations for enhanced pedal mobility, enabling climbing and headfirst descent from trees; these traits align with scansorial indices like high phalangeal and low robusticity values. Terrestrial cursorial multituberculates, including larger Cretaceous taxa like Repenomamus, exhibited symmetrical knee joints, stable ankle structures with malleoli, and moderately robust limb proportions suited for efficient ground traversal and asymmetrical gaits. This diversification, most pronounced among cimolodontan multituberculates, underscores their ecological versatility across Mesozoic and Cenozoic faunas. Habitat preferences shifted from humid Cretaceous settings to drier Paleogene environments, as reconstructed from fossil assemblages and associated paleosols. In the Late Cretaceous, multituberculates thrived in floodplain forests and wetland mosaics, such as those of the in , where burrow complexes and nesting sites indicate group burrowing behaviors in well-drained, vegetated floodplains near river systems. These habitats supported cohabitation with non-avian dinosaurs, including hadrosaurs and theropods, in which multituberculates occupied dominant small-mammal niches as opportunistic burrowers and foragers. By the Paleogene, particularly the Eocene, they adapted to temperate forest communities in northern basins like those in and , favoring wetter, Metasequoia-dominated woodlands but extending into more arid intermontane basins amid increasing climatic variability. Here, they coexisted with early and other eutherians, maintaining small-mammal dominance through niche partitioning in forested and basin-edge environments. Certain multituberculates evolved cheek teeth, reflecting adaptations to more abrasive vegetation in expanding open landscapes, though this predated the dominance of modern grasslands by millions of years and likely responded to grit-laden or fibrous plants in transitional forests. Body sizes, ranging from ~10 g mouse-like forms to ~1 kg beaver-sized individuals, scaled with these locomotor and habitat strategies, enabling niche exploitation across varied terrains.

Reproduction and Sociality

Multituberculates exhibited , giving birth to small, underdeveloped young, as evidenced by the narrow pelvic canal observed in well-preserved specimens from the . This reproductive mode aligns with the constraints imposed by their pelvic structure, which limited the size of offspring at birth to tiny, altricial forms requiring extensive post-natal care. Recent analyses of further indicate that multituberculates possessed a life history strategy more similar to that of placental mammals than marsupials, featuring prolonged periods and abbreviated . Such traits suggest extended maternal investment during , contrasting with the short gestation typical of marsupials. Growth patterns in multituberculates involved rapid post-natal development, as revealed by histological examination of long bones showing fast deposition rates akin to those in placental neonates. Dental annuli from and specimens confirm sigmoidal growth trajectories with elevated juvenile growth rates that declined upon reaching around 5-7 years of age. Evidence of appears in certain genera, potentially influencing reproductive behaviors through variations in body or dental features. Sociality is inferred from fossil assemblages, particularly the multituberculate Filikomys primaevus from Late Cretaceous deposits in Garfield County, Montana, where multiple individuals—including adults, subadults, and juveniles—were found in communal burrows. These bone beds suggest group-nesting and burrowing behaviors, indicative of multigenerational social structures that may have facilitated cooperative protection and resource sharing among family units. Such patterns parallel social organization in modern rodents, with possible parental care inferred from the presence of immature individuals alongside adults in these shared habitats. Comparisons to monotremes highlight convergent evolutionary pressures on early mammalian sociality, though multituberculates lacked the oviparity of that group.

Extinction

Timing and Last Records

Multituberculates persisted into the late Eocene, with their final confirmed occurrences dating to approximately 34 million years ago during the Chadronian North American Land Mammal Age (NALMA). The youngest known is Ectypodus childei, represented by dental remains from the White River Formation in , , marking the terminal phase of the group's presence in . These fossils tie into the broader of the Duchesnean and Chadronian NALMAs, spanning roughly 40–34 Ma, after which multituberculates vanish from the record. Post-Chadronian disappearance is well-established, with no verified records extending into the succeeding Orellan NALMA (approximately 34–32 Ma). Occasional reports of Oligocene multituberculates, such as purported finds in early Orellan strata, have been refuted based on reexamination of biostratigraphic context and specimen provenance, confirming the late Eocene as the definitive endpoint. In Asia, the group's persistence was limited to the Paleocene and early Eocene, with confirmed records only from the early Eocene Wutu Fauna in China, and no middle or late Eocene records extending their Laurasian persistence beyond North American timelines.

Proposed Causes

One longstanding hypothesis attributes the extinction of multituberculates to competitive exclusion by incoming , such as early forms resembling Ischyromys or Paramys, which overlapped in resource use for seeds and . This view posits that ' more efficient craniomandibular , including higher bite forces and broader dietary versatility, displaced multituberculates from shared niches starting in the late . However, a 2025 spatial study analyzing Eocene fossil associations found minimal niche overlap between multituberculates and , with multituberculates preferentially inhabiting specific humid, closed-canopy forests dominated by taxa like Nyssa and , while favored more open or mixed woodlands. This evidence critiques the competition model, suggesting coexistence for millions of years without direct exclusion. Environmental changes during the mid- to late Eocene, including and the shift from dense forests to open woodlands and savannas, likely reduced suitable habitats for folivorous and arboreal multituberculates. The Eocene-Oligocene transition involved a drop of approximately 4–8°C, driven by glaciation and declining atmospheric CO₂, which fragmented forested ecosystems and favored grasslands. Multituberculates, adapted to stable, humid subtropical forests of the late Eocene, faced niche contraction as these environments gave way to cooler, drier conditions by the early , limiting access to preferred foliage and fruits. This climatic forcing aligns with the timing of their final decline, contrasting with their survival through the more abrupt Cretaceous-Paleogene (K-Pg) event. Additional factors, such as ecological specialization, have been proposed as contributors, though evidence remains circumstantial. Contemporary models favor multi-causal explanations, integrating biotic interactions, climatic deterioration, and ecological specialization without a single dominant trigger. Unlike their resilience to the K-Pg mass extinction, where they radiated amid post-dinosaur opportunities, late Eocene multituberculates exhibited heightened vulnerability to gradual environmental perturbations. Critiques of the -blame emphasize that multituberculate waned before rodent diversification peaked, underscoring the role of abiotic stressors in their ultimate demise.

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