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Plasmogamy

Plasmogamy is the fusion of the cytoplasm (protoplasts) of two haploid cells in fungi, resulting in a shared cytoplasmic compartment containing genetically distinct nuclei without immediate nuclear fusion, thereby establishing a heterokaryotic or dikaryotic state.^[1]^[2] This process is a fundamental initial step in the sexual reproduction of many fungi, particularly in phyla such as Ascomycota and Basidiomycota, where it precedes karyogamy (nuclear fusion) and meiosis to generate genetic diversity through spore production.^[1]^[3] In the fungal life cycle, plasmogamy typically occurs between compatible mating types in heterothallic species or within the same thallus in homothallic ones, often involving specialized structures like hyphae or gametangia.^[1] It can manifest in various forms, including planogametic copulation (fusion of flagellated gametes), gametangial copulation (fusion of gametangia), spermatization (nuclear donation from spermatia to hyphae), and somatogamy (fusion of vegetative hyphal cells), allowing adaptation to diverse ecological niches.^[3]^[2] The resulting dikaryotic phase, prominent in Basidiomycota, enables prolonged co-existence and coordinated division of the two nuclei, enhancing mycelial growth and resilience before karyogamy occurs in specialized cells like basidia.^[1]^[2] Biologically, plasmogamy promotes genetic recombination and variability, which are essential for fungal adaptation, pathogenesis (e.g., in plant rusts like Puccinia graminis), and symbiotic interactions.^[2] This delayed nuclear fusion distinguishes fungal sexual cycles from those of animals or plants, providing a unique evolutionary strategy that has contributed to the ecological success of fungi as decomposers, pathogens, and mutualists.^[1]^[3]

Fundamentals

Definition

Plasmogamy is the fusion of the cytoplasms, or protoplasts, from two compatible haploid cells or hyphae in fungi, without the immediate fusion of their nuclei, resulting in a binucleate cell.^[1] This process unites the cytoplasmic contents while maintaining separate haploid nuclei within the shared compartment.^[4] The key outcome of plasmogamy is the formation of a heterokaryon or dikaryon, in which two genetically distinct haploid nuclei coexist and divide synchronously in the common cytoplasm, allowing for prolonged genetic diversity before nuclear fusion.^[2] In this state, the nuclei remain unfused, enabling the fungus to exploit dual genetic contributions during growth and development.^[5] Unlike typical fertilization in many eukaryotes, where cytoplasmic and nuclear fusion occur simultaneously, plasmogamy in fungi distinctly separates these events, with nuclear fusion (karyogamy) delayed, often until much later in the life cycle.^[1] This separation is a hallmark of fungal sexual reproduction, facilitating adaptive advantages through the dikaryotic phase.^[4] The term plasmogamy was introduced in the early 20th century within biological studies, including fungal contexts, to denote this initial cytoplasmic union as the first step in sexual reproduction.^[6]

Etymology

The term plasmogamy derives from the Ancient Greek plásma (πλάσμα), meaning "something molded" or "formed substance," alluding to the cytoplasm, and gámos (γάμος), meaning "marriage" or "union."^[7]^[8] The word was first recorded in English during the early 20th century, with the earliest documented use in 1912 by zoologist Edward A. Minchin to describe cytoplasmic fusion in protozoans; it was subsequently introduced into mycological literature around this period to denote the specific process of protoplasmic merging in fungal reproduction, separate from nuclear fusion.^[6]^[9] This nomenclature parallels karyogamy, which combines káryon (κάρυον, "nucleus") and gámos ("marriage"), emphasizing the distinction between cytoplasmic and nuclear unions in reproductive processes.

Mechanisms

In Lower Fungi

In lower fungi, such as those in Chytridiomycota and the former Zygomycota (now reclassified as Mucoromycota and Zoopagomycota), plasmogamy occurs through diverse morphological mechanisms that emphasize gamete-like structures or direct contact between reproductive cells, contrasting with the hyphal anastomosis typical in higher fungi.^[3]^[10] These methods facilitate the fusion of protoplasts from compatible individuals, initiating sexual reproduction without immediate nuclear fusion.^[4] Planogametic copulation represents one primary mode, involving the fusion of motile gametes in Chytridiomycota. Here, flagellated isogametes—morphologically similar cells—or anisogametes from compatible mating types unite, with the trend progressing from isogamy in basal lineages to oogamy (fusion of a small flagellated male gamete with a larger non-motile female gamete) in more derived groups.^[3] This process ensures cytoplasmic mixing while preserving nuclear separation initially, often leading to a brief dikaryotic state before karyogamy.^[3] In Mucoromycota, gametangial contact predominates, where non-motile gametangia from opposite mating strains, such as in Rhizopus stolonifer, grow toward each other and touch to form progametangia.^[11] Septa develop behind the contact point, isolating the gametangia, after which the intervening wall dissolves, allowing cytoplasm to exchange and form a multinucleate zygosporangium.^[11]^[12] This contact-based fusion minimizes exposure to environmental stress during plasmogamy.^[12] Spermatization provides an alternative in some Mucoromycota and Ascomycota lower groups, where a non-sexual hypha differentiates into a spermatium—a small, non-motile, uninucleate structure—that fuses with a receptive hypha or gametangium.^[13] For instance, in Pleurage anserina (Ascomycota), the spermatium attaches to and empties its contents into a receptive structure like the trichogyne, achieving protoplast union.^[14] This method, though less common in Mucoromycota than gametangial contact, highlights specialized male-like contributions to plasmogamy.^[13] Across these mechanisms in lower fungi, plasmogamy requires specificity through compatible mating types, typically designated as + and - strains, to prevent self-fusion and promote genetic diversity.^[4] Fusion occurs only between opposite types, resulting in an immediate or short-lived dikaryon where nuclei pair but do not fuse promptly, unlike the extended phase in advanced fungi.^[4]^[3]

In Higher Fungi

In higher fungi, primarily within the phyla Ascomycota and Basidiomycota, plasmogamy occurs through hyphal anastomosis, the fusion of compatible hyphae from different mating types. This process begins with the recognition and directed growth of hyphal tips toward each other, guided by chemotropic signals, followed by localized dissolution of cell walls and plasma membrane fusion to allow cytoplasmic mixing. Cytoplasm exchange can occur through septal pores in the fused hyphae, which permit the flow of organelles and nutrients while initially maintaining separate nuclei, or via direct wall breakdown in some cases.^[15]^[16] This mechanism is predominant in Ascomycota, where plasmogamy leads to the formation of ascogenous hyphae during sexual reproduction; for instance, in Neurospora crassa, fusion between a fertilizing conidium and the trichogyne of an ascogonium initiates dikaryotic ascogenous hyphae that grow within the developing ascocarp. In Basidiomycota, hyphal fusion establishes the dikaryotic state, often facilitated by clamp connections—specialized hyphal branches that fuse with the adjacent subapical cell to coordinate nuclear distribution during mitosis and ensure continued plasmogamy-like mixing. Examples include Ustilago maydis, where compatible sporidial hyphae fuse to form infectious dikaryotic mycelia, and mushroom-forming species like Coprinopsis cinerea, where clamp connections support extensive hyphal networks.^[4]^[15] Molecular triggers for hyphal anastomosis involve pheromone signaling for mate recognition and heterokaryon incompatibility systems to prevent fusion between genetically dissimilar strains. Pheromones, such as peptide ligands in N. crassa and U. maydis, bind to G-protein-coupled receptors on target hyphae, activating polarity pathways like Cdc42 to direct fusion. Heterokaryon incompatibility, controlled by multiple het loci, triggers programmed cell death if fusion occurs between incompatible individuals, ensuring only viable cytoplasmic mixing in compatible pairings.^[15]^[4] The outcome of plasmogamy in higher fungi is the development of extensive dikaryotic mycelia, where paired nuclei from different mating types coexist and proliferate within a shared cytoplasm, often forming interconnected networks that span large environmental areas, such as soil or wood substrates. This contrasts with gametangial fusion methods in lower fungi by emphasizing vegetative hyphal interactions over specialized gamete production.^[4]^[15]

Role in Fungal Reproduction

Dikaryotic Phase

The dikaryotic phase represents the intermediate stage in the life cycle of certain fungi following plasmogamy, characterized by the coexistence of two unfused haploid nuclei within a shared cytoplasm, resulting in binucleate cells that maintain genetic separation. These nuclei, derived from compatible mating types, undergo synchronized mitosis—dividing simultaneously during cell division—to preserve the binucleate condition across generations of hyphal growth. This phase is prevalent in Basidiomycota, where it constitutes the secondary mycelium responsible for extensive vegetative expansion, and in select Ascomycota, such as those forming ascogenous hyphae.^[17] The duration of the dikaryotic phase varies by fungal group and environmental cues but often extends for prolonged periods, ranging from weeks to months or even years, facilitating robust mycelial proliferation before transitioning to reproductive stages. In Basidiomycota, this extended timeline supports the formation of large fruiting bodies, while in Ascomycota, it is typically shorter yet critical for ascus maturation.^[17]^[18] Specialized cellular structures are essential for preserving the dikaryon. In Basidiomycota, dolipore septa—barrel-shaped cross-walls with central pores flanked by parenthesome membranes—regulate the migration of nuclei and organelles between hyphal compartments, preventing premature fusion while allowing coordinated division. Clamp connections, hook-like outgrowths at hyphal branch points, further ensure nuclear re-pairing post-mitosis. In Ascomycota, crozier formations at the tips of ascogenous hyphae create looped structures that position the two nuclei for synchronized divisions and eventual ascus development, thereby sustaining the dikaryotic state.^[17]^[19] From a genetic perspective, the dikaryotic phase promotes heterokaryotic variability, as the unpaired nuclei can harbor distinct alleles at multiple loci, fostering a form of genetic complementation that may enhance physiological adaptability without immediate recombination. This separation allows for the expression of cooperative gene interactions between the nuclei, potentially buffering against deleterious mutations and supporting diverse metabolic capabilities during growth.^[17]

Relation to Karyogamy

Karyogamy represents the fusion of the two haploid nuclei that coexist in the dikaryotic cell established following plasmogamy, resulting in the formation of a single diploid zygote nucleus.^[20] This nuclear pairing and fusion typically occurs in specialized reproductive cells, such as the basidium in Basidiomycota or the crozier leading to the ascus in Ascomycota.^[21] The dikaryon, as the precursor state to karyogamy, maintains nuclear independence during vegetative growth, delaying fusion until reproductive stages.^[5] The timing of karyogamy varies significantly among fungal groups, occurring shortly after plasmogamy in Mucoromycota and other early-diverging fungal lineages (formerly classified as Zygomycota), where nuclear fusion follows cytoplasmic mixing with minimal delay.^[5] In contrast, higher fungi like Ascomycota and Basidiomycota exhibit a prolonged postponement, with karyogamy happening only after extended dikaryotic mycelial growth, which can span days to years depending on environmental conditions.^[20] Karyogamy is generally localized within fruiting body structures, including basidia, asci, or zygospores, and is triggered by environmental cues such as nutrient limitation or stress that signal the shift to sporulation.^[22] This process immediately precedes meiosis in the diploid nucleus, which restores haploidy and generates recombinant haploid spores for dispersal.^[21]

Significance

Biological Importance

Plasmogamy plays a pivotal role in fungal reproduction by enabling the fusion of cytoplasm from two compatible haploid cells, thereby initiating the dikaryotic phase and facilitating genetic recombination through subsequent karyogamy and meiosis. This process allows fungi to combine genetic material from genetically diverse individuals, often from distant sources, which promotes outcrossing and increases genetic variability within populations. In species such as Coprinopsis cinerea, the multiallelic mating type loci enable outcrossing rates exceeding 50%, enhancing adaptability to environmental stresses and pathogens.^[4] Ecologically, the robust dikaryotic mycelial networks established post-plasmogamy are essential for key fungal functions, including nutrient cycling and symbiotic interactions. In basidiomycete decomposers, these networks drive the breakdown of complex organic matter like lignin in wood, recycling carbon and minerals in forest ecosystems and supporting soil health. Similarly, in ectomycorrhizal associations, dikaryotic hyphae form extensive extraradical networks that improve phosphorus and nitrogen uptake for host plants while providing fungi with carbohydrates, fostering mutualistic relationships that underpin forest productivity and resilience.^[23]^[24] In pathogenic contexts, plasmogamy is critical for virulence in rust fungi (Pucciniales), where fusion of compatible haploid cells produces dikaryotic hyphae that penetrate plant tissues, enabling infection and spore production for disease propagation. For instance, in wheat rust pathogens, this step advances the complex life cycle, allowing the fungus to exploit host resources and evade defenses, resulting in significant crop losses.^[25]^[26] The biological insights from plasmogamy have practical applications in biotechnology and agriculture. In mushroom cultivation, controlled plasmogamy between selected monokaryotic strains generates superior dikaryotic mycelia with enhanced vigor and yield, as seen in commercial production of edible species like Agaricus bisporus, optimizing fruiting body formation.^[27]

Evolutionary Aspects

Plasmogamy, the fusion of cytoplasmic contents without immediate nuclear fusion, likely originated in early fungal lineages as a mechanism to decouple cytoplasmic mixing from karyogamy, thereby permitting extended expression of haploid genomes before diploidization. This separation is considered a derived trait within the Fungi, contrasting with the ancestral diploid-dominant life cycles observed in many early-diverging lineages, where karyogamy follows plasmogamy rapidly to restore diploidy. Phylogenetic analyses of zoosporic fungi indicate that such prolonged haploid or dikaryotic phases emerged later, particularly in the subkingdom Dikarya, facilitating adaptive flexibility in nutrient-scarce environments.^[28]^[29] The dikaryotic phase resulting from plasmogamy offers evolutionary advantages by allowing masking of deleterious mutations through phenotypic dominance, where a functional allele from one nucleus can compensate for a defective one in the other, enhancing viability during vegetative growth. Additionally, this phase enables the testing of novel gene combinations across nuclei without committing to irreversible diploid fusion, promoting genetic diversity and purging harmful variants prior to meiosis and spore production. These benefits are particularly evident in long-lived dikaryons, which support robust mycelial expansion and resource acquisition in heterogeneous habitats.^[30]^[31]^[29] Variations in plasmogamy's duration and form occur across fungal phyla, reflecting adaptive divergences. In Chytridiomycota, an early-diverging group, plasmogamy is typically brief, with rapid karyogamy in the zygote, aligning with their aquatic, zoospore-based lifestyles and limited filamentous growth. In contrast, Basidiomycota exhibit an extended dikaryotic phase maintained by specialized structures like clamp connections, which may have coevolved with terrestrial colonization to optimize spore dispersal and substrate penetration in soil environments. Such prolongations are less pronounced in Ascomycota, where the dikaryon is often transient, highlighting phylum-specific innovations tied to ecological niches.^[28]^[29] Comparatively, fungal plasmogamy differs from the syngamy in animals and plants, where cytoplasmic and nuclear fusions occur simultaneously to form a diploid zygote immediately. This fungal strategy represents an evolutionary innovation suited to their filamentous, absorptive lifestyle, enabling sustained haploid-like expression and nuclear coordination during hyphal exploration, unlike the compact gamete unions in motile or sessile multicellular organisms of other kingdoms.^[29]

References

[1]
Basic Biology of Fungi - Medical Microbiology - NCBI Bookshelf - NIH
Normally plasmogamy (union of two hyphal protoplasts which brings the nuclei close together in the same cell) is followed almost immediately by karyogamy.
[2]
Plasmogamy - an overview | ScienceDirect Topics
In subject area: Agricultural and Biological Sciences. Plasmogamy is defined as the fusion of two haploid cells, resulting in a dikaryotic stage where two ...
[3]
BIO341 Lecture Topic 14 - University of Texas at Austin
Plasmogamy Terminology. Heterokaryosis - co-existence in the same cytoplasm of 2 or more genetically different nuclei*. * a dikaryon is a specialized ...
[4]
An Overview of the Function and Maintenance of Sexual ...
Sexual reproduction in fungi occurs in three stages. First, haploid cells of compatible mating types fuse (plasmogamy). This is followed by the fusion of the ...Missing: seminal | Show results with:seminal
[5]
plasmogamy, n. meanings, etymology and more
The earliest known use of the noun plasmogamy is in the 1910s. OED's earliest evidence for plasmogamy is from 1912, in the writing of Edward Minchin. plasmogamy ...
[6]
Plasma - Etymology, Origin & Meaning
From Greek plasma meaning "something molded," the word originates from plassein "to mold" and means form, shape, image, or counterfeit style.
[7]
-gamous - Etymology & Meaning of the Suffix
word-forming element meaning "marrying," from Greek gamos "marriage, a wedding" (see gamete) + -ous.
[8]
PLASMOGAMY Definition & Meaning - Dictionary.com
the fusion of the protoplasts of cells. Discover More. Word History and Origins. Origin of plasmogamy. First recorded in 1910–15; plasmo- + -gamy.
[9]
[PDF] Fungi.pdf
During the next stage the wall between the two gametangia breaks down and the cytoplasms of the two gametangia unite in a process called plasmogamy. Eventually ...
[10]
[PDF] Kingdom Fungi - PLB Lab Websites
(e) Septa form behind the tips of touching + and - hyphae, making gametangia. (f) Plasmogamy occurs when the walls between gametangia dissolve, making a ...
[11]
Mycology Glossary
spermation = little seed): a non-motile, uni-ucleate, spore-like male structure that empties its contents into a re-ceptive female structure during plasmogamy.
[12]
File: <plate1
Plate 61 = Sexual reproduction: Plasmogamy thru' gametangial copulation in Sporodinia garndis. Plate 62 = Sexual reproduction: Plasmogamy by spermatization in ...
[13]
Chemotropism and Cell-Cell Fusion in Fungi - PMC - NIH
For example, hyphae that result from mating in many basidiomycete fungi are dikaryotic (2), and the maintenance of this state involves the development of clamp ...
[14]
SO, a Protein Involved in Hyphal Fusion in Neurospora crassa ...
Septal pores allow cytoplasm and organelles, including nuclei, to move between hyphal compartments, thus making the fungal colony a syncytium. The syncytial ...N. Crassa Strains And Growth... · So-Gfp Fusions Are... · So-Gfp Localization To...
[15]
https://pmc.ncbi.nlm.nih.gov/articles/PMC8826960/
[16]
[PDF] Fungi - The PhycoLab
Plasmogamy. 2. Karyogamy. 3. Meiosis. • Dikaryon: after plasmogamy two haploid nuclei do not fuse for some time. (retarding karyogamy for months or years!)
[17]
7.4 The dikaryon - David Moore's World of Fungi
The dikaryotic state is maintained by a specialised cell biology: the nuclei divide together (= conjugate mitosis) and at the same time a 'clamp connection' ...Missing: phase characteristics crozier review
[18]
Fungal Sex: The Basidiomycota - PMC - NIH
In all these and other basidiomycetes, nuclear fusion (karyogamy) is usually delayed until the formation of basidia (or teliospores). Meiosis ensues, generating ...
[19]
A non-pheromone GPCR is essential for meiosis and ... - PNAS
Oct 9, 2023 · Dikaryotic cells formed by mating and plasmogamy can grow inside developing ascocarps, and karyogamy occurs only in croziers that are formed at ...
[20]
https://pmc.ncbi.nlm.nih.gov/articles/PMC5467461/
[21]
Signal Transduction Cascades Regulating Fungal Development and ...
Abstract. Cellular differentiation, mating, and filamentous growth are regulated in many fungi by environmental and nutritional signals.
[22]
Basidiomycota - Soil Ecology Wiki
Mar 31, 2023 · The Basidiomycota are of great ecological importance particularly in forest ecosystems due to their ability to decompose and recycle nutrients, ...
[23]
Chapter 11 ~ Fungi – Forest Ecology
The result of the plasmogamy step is four basidiospores. Karyogamy results directly in the formation of mycelia. A basidiocarp is the fruiting body of a ...Missing: earliest mention literature
[24]
[PDF] "The Rust Fungi". In: Encyclopedia of Life Sciences (ELS) - USDA ARS
Sep 15, 2009 · in fertilization (Figure 1c), also called plasmogamy (the uniting of two cells to form a single cell with two genetically distinct, haploid ...
[25]
Sexual recombination in cereal rust fungi: Knowns and unknowns of ...
Feb 4, 2025 · Plasmogamy (cell fusion) occurs when pycniospores fuse with receptive hyphae of opposing mating types (MAT) from different pycnia. This fusion ...
[26]
Review on mushroom mycelium-based products and their ...
Jan 10, 2025 · This treatment can yield mutant monokaryotic mycelia, which are then selected and mated to produce dikaryons with improved characteristics.<|separator|>
[27]
The good, the bad and the tasty: The many roles of mushrooms
Dikaryotic mycelium forms when two sexually compatible monokaryotic hyphae fuse in a process called plasmogamy (Kues, 2000, Raudaskoski and Kothe, 2010). Unlike ...
[28]
Diploid-dominant life cycles characterize the early evolution of Fungi
Dikaryotic fungi, particularly the mushroom fungi with dikaryotic phases dominating the life cycle, may have both the advantages of diploidy and the advantages ...
[29]
The Evolution of Sex: a Perspective from the Fungal Kingdom - PMC
We will discuss the sexual development of fungi, transitions between self-fertility (homothallism) and cross-fertility (heterothallism), and the mating type ...
[30]
Mosaic fungal individuals have the potential to evolve within a single ...
Oct 19, 2020 · The origin of dikaryotic hyphae in fruit bodies of Armillaria borealis and A. tabescens developed in pure culture. In Proceedings 8th ...<|separator|>
[31]
Modeling the consequences of the dikaryotic life cycle of mushroom ...
... plasmogamy ... This unique form of mating between a dikaryon and monokaryon is known as di-mon mating, often called Buller mating (Buller, 1934; Quintanilha, 1938) ...