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Schaffer collateral

The Schaffer collaterals, named after the Hungarian anatomist Károly Schaffer, who described this pathway in 1892,^[1] are axon collaterals that originate from pyramidal neurons in the cornu ammonis 3 (CA3) region of the hippocampus and project unidirectionally to the cornu ammonis 1 (CA1) region, where they form excitatory glutamatergic synapses primarily on the apical dendrites of CA1 pyramidal neurons in the stratum radiatum.^[2]^[3] These projections constitute a critical segment of the hippocampal trisynaptic circuit—linking the dentate gyrus (DG) via CA3 to CA1—and serve as the primary pathway for transmitting processed information from CA3 to CA1, enabling associative memory formation and spatial learning.^[3]^[2] Anatomically, the Schaffer collaterals arise from the axons of CA3 pyramidal cells, which branch extensively to innervate both ipsilateral and, to a lesser extent, contralateral CA1 regions, creating asymmetrical synapses on dendritic spines using glutamate as the neurotransmitter.^[2]^[3] Physiologically, these synapses are highly plastic, supporting mechanisms such as long-term potentiation (LTP)—induced by high-frequency stimulation (e.g., 100 Hz for 1 second)—which enhances synaptic strength through increased AMPA receptor trafficking and postsynaptic calcium influx, and long-term depression (LTD) via low-frequency stimulation (e.g., 1-5 Hz).^[2]^[3] This plasticity is pivotal for hippocampal-dependent memory consolidation, as disruptions in Schaffer collateral function have been implicated in models of epilepsy, traumatic brain injury, and cognitive deficits in conditions like depression.^[3] A notable feature of Schaffer collateral synapses is their distance-dependent scaling across the CA1 dendritic arbor: synaptic conductances increase progressively from proximal (near the soma) to distal locations, primarily due to higher postsynaptic densities of AMPA receptors (e.g., approximately 91 channels proximally versus 171 distally), which compensates for electrotonic filtering and normalizes somatic excitatory postsynaptic potential (EPSP) amplitudes.^[4] This scaling ensures efficient signal propagation without presynaptic modifications in release probability or glutamate dynamics, highlighting the pathway's role in maintaining uniform excitatory drive throughout the neuron.^[4] Research on these synapses, dating back to foundational LTP studies in the 1980s, continues to inform broader understandings of neural computation and therapeutic targets for memory disorders.^[2]

Anatomy and Development

Location and Morphology

The Schaffer collaterals are axonal projections originating from pyramidal neurons in the CA3 region of the hippocampus, extending primarily to the CA1 region to form excitatory synapses on local pyramidal neurons and interneurons. These collaterals constitute the primary output pathway from CA3, traversing the hippocampal formation transversely from CA3 to CA1 while also exhibiting some longitudinal spread along the septotemporal axis.^[5]^[6] Morphologically, the axons of CA3 pyramidal cells emerge from the stratum pyramidale, where they initially branch to form recurrent collaterals within CA3 before giving rise to the Schaffer collaterals that course through the stratum radiatum toward CA1. Upon reaching CA1, these unmyelinated axons, with diameters of approximately 0.17 μm, continue to branch extensively in the stratum radiatum and stratum oriens, forming thin, tubular shafts interspersed with oblong varicosities (boutons) that measure about 1.1 μm in length and 0.4 μm in diameter. These varicosities, spaced roughly 3 μm apart, include en passant and terminal types, with 68% being single-synapse boutons and 19% multiple-synapse boutons containing 2–4 postsynaptic densities.^[5]^[6]^[7] The primary synaptic targets of Schaffer collaterals are the dendrites of CA1 pyramidal neurons, with synapses forming on apical dendrites in the stratum radiatum and basal dendrites in the stratum oriens. Additionally, these axons target inhibitory interneurons, including cholecystokinin-positive basket cells, which receive inputs on their somata and proximal dendrites, and Schaffer collateral-associated cells, which are contacted along their dendritic arbors across CA1 layers.^[5]^[8]^[9] Visualization of Schaffer collateral morphology has historically relied on the Golgi staining method for light microscopy to reveal the overall branching patterns and axonal trajectories from individual CA3 neurons. More detailed ultrastructural analysis employs serial electron microscopy with three-dimensional reconstruction, which delineates the precise arrangement of axonal shafts, varicosities, and synaptic contacts.^[6]^[7] In rodents, individual Schaffer collateral branches typically extend 1–2 mm in length, reflecting the transverse distance between CA3 and CA1 subfields. Each CA3 pyramidal neuron generates thousands of synapses via these collaterals, with estimates indicating up to several thousand contacts per axon arbor in CA1.^[5]^[10]

Embryonic and Postnatal Development

The Schaffer collaterals originate from axonal outgrowth of CA3 pyramidal neuron progenitors in the embryonic rodent hippocampus, beginning around embryonic day 15-18 (E15-E18), when postmitotic CA3 neurons extend initial axons toward the CA1 region.^[11] This outgrowth is guided by molecular cues, including netrin-1, which attracts developing CA3 axons to their targets in CA1, and slit proteins, such as Slit2 expressed in CA3, which provide repulsive signals to refine pathfinding and prevent aberrant midline crossing.^[12]^[13] Transcription factors like Emx2 play a crucial role in regulating hippocampal growth and maturation during this phase, ensuring proper progenitor proliferation and areal patterning.^[14] Guidance molecules such as Ephrin-B, expressed on astrocytes and neurons, further contribute to axonal navigation and early collateral branching by modulating repulsive and adhesive interactions.^[15] Key developmental stages involve initial pathfinding from CA3 to CA1 during late embryogenesis, followed by collateral sprouting and synapse formation in early postnatal life. By postnatal day 5-10 (P5-P10), functional glutamatergic synapses form at Schaffer collateral-CA1 contacts, initially as NMDA receptor-dominant "silent" synapses that transition to AMPA receptor-containing mature synapses through activity-dependent mechanisms.^[16] This process is refined by spontaneous network activity, such as giant depolarizing potentials peaking around P7, which drive axonal collateralization and target selection.^[11] Postnatal maturation includes synapse elimination during the first week, where excess connections are pruned via apoptosis peaking at P4, and strengthening through upregulated NMDA receptor expression, enabling long-term potentiation-like mechanisms by P12.^[11]^[17] Myelination of Schaffer collateral axons begins around the second to third postnatal week, with mature myelin sheaths appearing in the stratum radiatum by P14-P21, supporting faster conduction in the maturing circuit.^[18] In comparison to rodents, where these events unfold over weeks, hippocampal development in primates exhibits prolonged timelines, with axonal and synaptic maturation extending over months to years due to larger brain size and extended gestation.^[19] Disruptions in development, such as in reelin knockout mutants (reeler mice), lead to aberrant hippocampal lamination and misplaced CA3 neurons, resulting in disorganized Schaffer collateral projections and reduced connectivity to CA1, often accompanied by altered synaptic release probability.^[20]^[21]

Function in the Hippocampus

Synaptic Connections and Transmission

The Schaffer collaterals, originating from CA3 pyramidal neurons, form excitatory synapses primarily onto the dendritic spines of CA1 pyramidal neurons in the stratum radiatum of the hippocampus, releasing glutamate as the principal neurotransmitter. This glutamate binds to postsynaptic ionotropic receptors, including AMPA and NMDA subtypes, which mediate fast excitatory transmission. AMPA receptors are responsible for the initial rapid depolarization, while NMDA receptors contribute to longer-lasting components under conditions of sufficient postsynaptic depolarization to relieve their magnesium block.^[22]^[23] Presynaptically, action potentials propagate along the unmyelinated axons of the Schaffer collaterals, reaching terminal boutons where voltage-gated calcium channels open, allowing Ca²⁺ influx that triggers vesicular glutamate release through SNARE complex-mediated exocytosis. The resulting excitatory postsynaptic potentials (EPSPs) exhibit fast kinetics, with typical rise times of approximately 2 ms and decay times of approximately 8-9 ms for the AMPA receptor-mediated component, enabling precise temporal signaling in hippocampal circuits.^[24] Postsynaptically, activation of AMPA receptors primarily permits Na⁺ influx, driving rapid depolarization, whereas NMDA receptors allow both Na⁺ and Ca²⁺ entry, contributing to synaptic integration; modulation occurs via presynaptic and postsynaptic metabotropic glutamate receptors (mGluRs), particularly group II and III subtypes, which fine-tune release probability and receptor sensitivity without directly gating ions. The baseline strength and reliability of transmission at these synapses are characterized by a low release probability of approximately 0.2-0.3 per action potential in acute hippocampal slices, reflecting the probabilistic nature of vesicular fusion and ensuring variability in synaptic output. This probability is assessed through measures such as the paired-pulse ratio or coefficient of variation in evoked responses, with failures indicating stochastic release events. Experimental characterization relies on in vitro hippocampal slice electrophysiology, where a stimulating electrode is placed in the Schaffer collateral pathway in the CA3 region, and field excitatory postsynaptic potentials (fEPSPs) or whole-cell currents are recorded in the CA1 stratum radiatum using a nearby electrode; stimuli are typically delivered at low frequencies (0.033-0.1 Hz) to monitor baseline transmission without inducing plasticity.^[25]^[26]

Role in Hippocampal Circuitry and Information Processing

The Schaffer collaterals form a critical link in the hippocampal trisynaptic pathway, connecting the auto-associative network of CA3 pyramidal cells to CA1 pyramidal cells, thereby facilitating pattern completion and enabling output to the subiculum and entorhinal cortex for broader cortical integration.^[27] This pathway originates from entorhinal cortex layer II inputs to the dentate gyrus, which then project via mossy fibers to CA3, where recurrent collaterals support associative memory storage before relaying refined representations to CA1 via the Schaffer collaterals.^[28] Computationally, the Schaffer collaterals relay sparse coding from CA3—characterized by low firing rates and high pattern orthogonality—to CA1, where it contributes to conjunctive representations that integrate spatial and contextual elements essential for episodic memory encoding.^[29] This transfer supports the transformation of incomplete or partial inputs into complete memory traces, with CA1 serving as a comparator that matches CA3 patterns against direct entorhinal inputs for novelty detection and consolidation.^[30] Within the hippocampal network, Schaffer collateral inputs engage feedforward inhibition through activation of interneurons such as basket cells and Schaffer-associated interneurons, which temporally align excitatory drive to CA1 pyramidal cells and prevent overexcitation.^[31] Additionally, these inputs interact with recurrent excitation in CA1, where local collateral connections among pyramidal cells amplify coherent activity patterns while maintaining network stability.^[32] In vivo, Schaffer collaterals synchronize with theta rhythms (4-8 Hz), modulating place cell firing in CA1 during spatial navigation tasks, where they enhance phase precession and support the sequential reactivation of trajectories for path integration.^[33] This rhythmic entrainment ensures precise timing of CA3-driven inputs relative to behavioral epochs, contributing to the stability of place field representations in familiar environments.^[34] From an information theory perspective, transmission across Schaffer collaterals efficiently encodes probabilistic memory associations with low redundancy, based on firing rate correlations between CA3 and CA1 populations.^[30] Disruption of Schaffer collateral projections underlies pathological conditions, including models of temporal lobe epilepsy where hyperexcitability leads to aberrant synchronization and seizure propagation, and Alzheimer's disease, where tau pathology impairs axonal integrity and reduces input to CA1, exacerbating memory deficits.^[35]^[36]

Synaptic Plasticity

Long-Term Potentiation (LTP)

Long-term potentiation (LTP) represents a form of Hebbian synaptic plasticity characterized by a long-lasting increase in the strength of synaptic transmission at Schaffer collateral-CA1 synapses in the hippocampus, following brief high-frequency stimulation. This phenomenon was first discovered in 1973 by Bliss and Lømo, who observed persistent potentiation in the perforant path to dentate gyrus synapses of the rabbit hippocampus. The Schaffer collateral-CA1 synapse subsequently became a primary model system for studying synaptic plasticity. LTP is typically induced by tetanic stimulation at frequencies around 100 Hz for 1 second, which triggers a robust enhancement of excitatory postsynaptic potentials (EPSPs) that endures for more than 1 hour.^[37] LTP at these synapses manifests in two temporally distinct phases: early LTP (E-LTP) and late LTP (L-LTP). E-LTP, lasting less than 1 hour, is independent of new protein synthesis and relies on post-translational modifications of existing proteins, such as phosphorylation events that stabilize synaptic changes. In contrast, L-LTP persists for several hours or longer and requires de novo transcription and translation of plasticity-related proteins, enabling more enduring structural and functional alterations at the synapse. The core induction mechanism of LTP involves calcium influx through NMDA receptors, which is facilitated by coincident presynaptic glutamate release and postsynaptic depolarization that relieves the magnesium block of these receptors. This calcium entry activates calcium/calmodulin-dependent protein kinase II (CaMKII), a key enzyme that autophosphorylates and translocates to the synapse, where it phosphorylates targets to promote synaptic strengthening. Downstream, CaMKII activation drives the trafficking and insertion of AMPA receptors into the postsynaptic membrane, increasing the number of these ion channels available for glutamate binding and thereby enhancing synaptic efficacy.^[37] In experimental settings, LTP is commonly studied in acute hippocampal slices from rodents, where Schaffer collaterals are stimulated in the CA3 region and field EPSPs are recorded in the stratum radiatum of CA1. High-frequency tetani or theta-burst stimulation—short bursts of action potentials at theta rhythm frequencies (4-7 Hz), mimicking in vivo hippocampal oscillations—reliably induce LTP, often resulting in a 50-200% increase in the slope of field EPSPs relative to baseline, providing a quantifiable measure of synaptic potentiation.^[38]

Short-Term Plasticity

Short-term plasticity at Schaffer collateral synapses refers to reversible, transient alterations in synaptic efficacy that occur over milliseconds to seconds following brief patterns of presynaptic activity, enabling dynamic adjustment of signal transmission in the hippocampal CA1 region. One primary form is paired-pulse facilitation (PPF), observed when two closely spaced action potentials (interpulse intervals <50 ms) evoke a second excitatory postsynaptic potential (EPSP) or current (EPSC) that is enhanced relative to the first. This enhancement arises presynaptically from residual Ca²⁺ accumulation in the nerve terminal after the initial stimulus, which elevates the probability of vesicular neurotransmitter release (P_r) for the subsequent pulse.^[39] PPF peaks at intervals around 15-30 ms, with typical ratios of the second to first EPSP amplitude ranging from 1.5 to 2.0 under standard recording conditions (2 mM extracellular Ca²⁺).^[39]^[40] In contrast, paired-pulse depression emerges at longer interpulse intervals (typically >100 ms) or during higher-frequency trains, where the second response is diminished due to partial depletion of the readily releasable pool of synaptic vesicles by the first stimulus, limiting glutamate availability for the second release event.^[41] This presynaptic mechanism involves modulation of P_r, with the baseline P_r at Schaffer collateral-CA1 synapses estimated at approximately 0.2 under physiological conditions, favoring facilitation over depression during sparse activity but shifting toward depletion under sustained demand.^[42] Experimental assessment of these dynamics commonly employs paired-pulse protocols, quantifying the ratio of second-to-first EPSP slopes or amplitudes from field or whole-cell recordings in hippocampal slices.^[40] Several factors modulate these processes: elevated temperature (e.g., 32-34°C versus 22-24°C) amplifies facilitation by accelerating Ca²⁺ dynamics and recovery kinetics; higher extracellular Mg²⁺ concentrations reduce Ca²⁺ influx through voltage-gated channels, lowering baseline P_r and thereby enhancing PPF magnitude; and synapses with inherently low initial P_r exhibit greater facilitation, as residual Ca²⁺ has a proportionally larger impact on release.^[43]^[44]^[45] Functionally, short-term plasticity at these synapses adapts transmission to presynaptic firing patterns, such as enhancing reliability during high-frequency bursts that mimic in vivo theta or sharp-wave activity, thereby optimizing information flow through the trisynaptic hippocampal circuit without inducing persistent changes.^[39] Compared to mossy fiber-CA3 synapses, Schaffer collateral connections display higher paired-pulse facilitation owing to their lower baseline P_r (~0.2), whereas mossy fibers, with higher initial P_r, tend toward depression in paired-pulse paradigms but show distinct frequency-dependent potentiation.^[46]^[42]

LTP at Schaffer Collateral-CA1 Synapses and SK2 Channel Plasticity

Small-conductance Ca²⁺-activated potassium (SK2) channels are expressed in the postsynaptic density of dendritic spines on hippocampal CA1 pyramidal neurons, where they are closely coupled to NMDA receptors, typically within 25 nm. These channels open in response to Ca²⁺ influx through NMDA receptors, generating a hyperpolarizing afterpotential that limits further depolarization and Ca²⁺ entry, thereby raising the threshold for LTP induction at Schaffer collateral-CA1 synapses.^[47] LTP induction at these synapses triggers a pathway-specific downregulation of SK2 channel activity through PKA-dependent phosphorylation at serine residues 568-570 on the channel's C-terminal domain, leading to rapid internalization from the postsynaptic density into the spine interior. This process reduces the SK2-mediated afterhyperpolarization, thereby enhancing excitatory postsynaptic potentials (EPSPs) by approximately 13% and amplifying dendritic Ca²⁺ transients to support LTP expression. The internalization is activity-dependent, requiring coincident presynaptic stimulation and postsynaptic depolarization, and is blocked by PKA inhibitors such as H89 or KT5720, or by a competing peptide mimicking the phosphorylation sites.^[47] Experimental studies demonstrate that blocking SK channels with apamin (100 nM) increases the EPSP slope by 41% in naive pathways but has no effect in already potentiated synapses, indicating that LTP effectively silences SK2 function to mimic this blockade. This SK2 plasticity complements the canonical NMDA receptor-CaMKII pathway by sustaining elevated Ca²⁺ levels critical for late-phase LTP maintenance, without altering AMPA receptor trafficking directly.^[47] The loss of SK2 activity is detectable within 40 minutes following theta-burst stimulation and persists for several hours, peaking in suppression between 30 and 60 minutes post-induction, thereby facilitating long-lasting enhancements in synaptic strength specific to the Schaffer collateral-CA1 pathway.^[47]

Presynaptic Mechanisms

Vesicular Neurotransmitter Release

The vesicular release of glutamate at Schaffer collateral terminals follows a tightly regulated cycle involving docking, priming, and fusion of synaptic vesicles. Synaptic vesicles dock at the presynaptic active zone through interactions mediated by proteins such as RIM and Munc13, which organize the release machinery.^[48] Priming of docked vesicles into a fusion-competent state is facilitated by the formation of SNARE complexes composed of syntaxin-1, SNAP-25, and VAMP2 (also known as synaptobrevin-2), which zipper together to bring the vesicle and plasma membranes into close apposition.^[49] Fusion is then triggered by calcium influx during an action potential, where synaptotagmin-1 acts as the primary Ca²⁺ sensor, binding Ca²⁺ ions and accelerating SNARE-mediated membrane merger in a process known as the electrostatic switch mechanism.^[50] The readily releasable pool (RRP) represents the subset of primed vesicles available for immediate exocytosis, estimated at 1-10 vesicles per Schaffer collateral terminal based on ultrastructural and functional assays, with recent optical analyses reporting medians around 3.^[25]^[51]^[52] This pool size is commonly assessed using brief applications of hypertonic sucrose (e.g., 0.5-0.8 Osm), which evoke release independently of Ca²⁺ influx by inducing osmotic swelling and mechanical fusion of primed vesicles.^[53] Release occurs primarily at en passant boutons along the unmyelinated axons, where active zones are enriched with Munc13 and RIM proteins that stabilize vesicle docking and priming.^[54]^[48] Quantal analysis of transmission at these synapses reveals that miniature excitatory postsynaptic currents (mEPSCs), reflecting spontaneous single-vesicle release, have amplitudes around 10 pA under standard recording conditions.^[4] Evoked release typically involves a quantal content of 1-5 vesicles per action potential, with single-vesicle release predominant under basal conditions and multivesicular release emerging at higher Ca²⁺ levels or facilitation.^[52] Electron microscopy studies confirm the presence of clear-core synaptic vesicles (~40 nm diameter) loaded with glutamate and occasional dense-core vesicles (~80 nm) containing neuropeptides, with vesicle recycling occurring via clathrin-mediated endocytosis near the active zone to replenish the pool efficiently.^[55]^[56] The priming process is energetically demanding, relying on maintenance of a high ATP/ADP ratio to support SNARE complex assembly and chaperone activities, with ATP hydrolysis essential for converting docked vesicles into the releasable state.^[57] Disruptions in energy supply, such as during intense activity, can limit RRP refilling and thus constrain sustained release at Schaffer collateral terminals.^[58]

Regulation of Release Probability

The release probability (P_r) at Schaffer collateral-CA1 synapses, typically estimated at 0.15-0.25 under basal conditions, can be quantified using the progressive blockade of postsynaptic NMDA receptors by MK-801 during repetitive stimulation, which reveals cumulative release profiles and initial quantal content.^[59] This method exploits the use-dependent entry of MK-801 into open NMDA channels, allowing estimation of presynaptic release efficiency without directly perturbing the terminal.^[60] Presynaptic modulation of P_r occurs through retrograde messengers released from CA1 pyramidal cells, which diffuse back to alter transmitter release during synaptic plasticity. Nitric oxide (NO), generated postsynaptically via nNOS activation following NMDA receptor stimulation, acts as a retrograde signal to induce long-term depression (LTD) by reducing P_r at Schaffer collateral terminals, likely via cGMP-dependent pathways that downregulate calcium influx or vesicle priming.^[61] Similarly, endocannabinoids such as 2-arachidonoylglycerol (2-AG), mobilized by postsynaptic mGluR1/5 or theta-burst activity, bind presynaptic CB1 receptors to transiently suppress glutamate release, thereby lowering P_r and contributing to depolarization-induced suppression of inhibition or excitation in hippocampal circuits.^[62] Calcium dynamics critically regulate P_r, with P/Q-type (Ca_v2.1) voltage-gated calcium channels serving as the primary source of Ca^{2+} influx triggering vesicular release at these synapses, while N-type channels play a supportive role.^[63] Intracellular Ca^{2+} buffering, mediated by endogenous proteins like calbindin-D28k, modulates the spatiotemporal profile of Ca^{2+} near release sites; saturation of these buffers during high-frequency activity facilitates paired-pulse potentiation by elevating local Ca^{2+} and thus increasing P_r for subsequent stimuli.^[64] Homeostatic scaling maintains network stability by activity-dependent adjustments to P_r, often compensating for chronic changes in postsynaptic excitability. In chronic activity blockade models, enhanced Schaffer collateral drive restores CA1 firing rates through presynaptic upregulation of P_r.^[65] Pharmacological influences further modulate P_r; activation of presynaptic adenosine A1 receptors by ambient adenosine tonically depresses glutamate release at Schaffer collateral synapses, reducing P_r and contributing to activity-dependent short-term depression.^[66] Conversely, serotonin via 5-HT4 receptors enhances synaptic transmission at Schaffer collateral-CA1 synapses, with evidence suggesting presynaptic facilitation of release probability in hippocampal circuits, though the precise locus requires further delineation.^[67] In pathological contexts, such as Alzheimer's disease models, beta-amyloid oligomers impair presynaptic function by suppressing P_r at Schaffer collateral-CA1 synapses through mechanisms involving reduced voltage-gated calcium channel activity and disrupted vesicle priming, leading to diminished excitatory drive and cognitive deficits.^[68]

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Oct 13, 2020 · The presynaptic bouton of the hippocampal mossy fiber (MF) is much larger in size than that of the Schaffer collateral (SC) synapse. Here we ...
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SK2 channel plasticity contributes to LTP at Schaffer collateral–CA1 ...
Jan 20, 2008 · SK2 channels in CA1 modulate the induction of synaptic plasticity and the acquisition of learning, likely by influencing Ca 2+ entry during neuronal activity ...
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RIM-BP2 primes synaptic vesicles via recruitment of Munc13 ... - eLife
Sep 19, 2019 · RIM-BP2 has a substantial impact on neurotransmitter release by promoting vesicle docking/priming and vesicular release probability via stabilization of Munc13 ...Missing: passant | Show results with:passant
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SNAP-25 isoforms differentially regulate synaptic transmission and ...
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Synaptotagmin Ca2+ Sensors and Their Spatial Coupling to ...
Ultrafast glutamate sensors resolve high-frequency release at Schaffer collateral synapses. ... Models of synaptotagmin-1 to trigger Ca2+ -dependent vesicle ...
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Long-Term Depression of Presynaptic Release from the Readily ...
Hypertonic shock (25 sec of 800 mOsm ACSF plus sucrose) selectively loads the RRP, which represents the ∼28% of total vesicle pool brightness with more rapid ...
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https://pmc.ncbi.nlm.nih.gov/articles/PMC6741233/
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Vesicular release probability sets the strength of individual Schaffer ...
Oct 17, 2022 · Here, we optically measure evoked transmitter release at individual Schaffer collateral synapses at different calcium concentrations, using the ...
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Ultrastructural Correlates of Presynaptic Functional Heterogeneity in ...
Mar 17, 2020 · Vesicles that contained a prominent electron-dense core were considered dense-cored vesicles (DCVs). For illustrative purposes, figures ...
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Ultrastructural and functional fate of recycled vesicles in ... - Nature
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Dec 19, 2016 · Docking of synaptic vesicles does not appear to require ATP; however, priming them for subsequent release is an ATP-dependent process (Yao ...
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In vivo‐like stimulation triggers the release of substantially more vesicles in Schaffer collateral boutons from brain slice cultures in vitro (Rose et al ...
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Jan 4, 2006 · We suggest that MVR occurs at SC–CA1 synapses when P r is elevated by facilitation and that MVR may be a phenomenon common to many synapses throughout the CNS.
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Estimating the Distribution of Synaptic Reliabilities
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CA1 Pyramidal Cell Theta-Burst Firing Triggers Endocannabinoid ...
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Presynaptic Cav2.1 and Cav2.2 Differentially Influence Release ...
Nov 17, 2004 · The kinetics and modulation of Cav2.1 and Cav2.2 channels differ and may affect presynaptic calcium influx. We compared release dynamics at CA3/ ...
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The Self-Tuning Neuron: Synaptic Scaling of Excitatory Synapses
Homeostatic synaptic scaling is a form of synaptic plasticity that adjusts the strength of all of a neuron's excitatory synapses up or down to stabilize firing.
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Adenosine A1 Receptor-Mediated Synaptic Depression in the ...
Jun 15, 2020 · ... A1Rs would induce synaptic depression in Schaffer collateral inputs to CA2 and CA1. Importantly, we tested whether transmission at these ...
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Amyloid β oligomers suppress excitatory transmitter release via ...
Mar 13, 2019 · In the present study, we report that the release probability (Pr) at the synapse between the Schaffer collateral (SC) and CA1 pyramidal neurons ...