Anethole is an organic compound with the molecular formula C₁₀H₁₂O, classified as a monomethoxybenzene substituted by a prop-1-en-1-yl group at position 4, and its IUPAC name is 1-methoxy-4-[(E)-prop-1-enyl]benzene.[1] It exists primarily as the trans-isomer, which is the most abundant and stable form, with a molecular weight of 148.20 g/mol and a structure featuring a phenyl ring attached to a methoxy group and a propenyl side chain.[1][2]Naturally occurring as a major constituent in essential oils from plants such as anise (Pimpinella anisum), fennel (Foeniculum vulgare), and star anise (Illicium verum), anethole imparts the characteristic sweet, anise-like aroma and flavor to these sources.[1][3] Commercially, it is often produced synthetically or extracted from turpentine-derived sources, and it has been recognized as generally safe (GRAS) for use in foods by regulatory authorities.[3] Physically, anethole appears as a colorless to pale yellow liquid or white crystals with a melting point of 21.3°C, boiling point of 234°C, and density of 0.9882 g/cm³, exhibiting low water solubility but good solubility in ethanol and other organic solvents.[1]Anethole's primary applications include flavoring and fragrance in foods, beverages (such as absinthe and ouzo, where it contributes to the "ouzo effect" of milky emulsions), cosmetics, soaps, and oral care products, as well as an excipient in pharmaceuticals for masking unpleasant tastes, though high doses can pose toxicity risks, including potential allergic skin reactions and moderate acute toxicity in non-human species (e.g., LD₅₀ of 2090 mg/kg orally in rats).[1][3][4][5]
Chemical Properties
Molecular Structure and Isomers
Anethole possesses the molecular formula C₁₀H₁₂O and has a molecular weight of 148.23 g/mol.[6] Its IUPAC name is 1-methoxy-4-[(E)-prop-1-en-1-yl]benzene for the predominant isomer, reflecting its structure as a derivative of anisole with a propenyl substituent. Classified as a phenylpropanoid, anethole features a benzene ring with a methoxy group (-OCH₃) attached at the 1-position and a 1-propenyl side chain (-CH=CH-CH₃) at the para position (4). This arrangement results in a conjugated system where the double bond in the propenyl chain extends the π-system from the aromatic ring, influencing electronic properties and reactivity.The key structural feature enabling isomerism is the carbon-carbon double bond in the propenyl group, which restricts rotation and gives rise to geometric (E/Z or trans/cis) isomers. In the (E)- or trans-anethole, the higher-priority groups—the phenyl ring and the methyl group—are positioned on opposite sides of the double bond, leading to a more extended, stable conformation.[7] Conversely, the (Z)- or cis-anethole has these groups on the same side, resulting in a more compact structure. The trans isomer predominates in natural sources, often comprising over 90% of anethole content in essential oils from plants like anise and fennel.[8]In terms of basic reactivity, anethole behaves as both an alkene, susceptible to electrophilic additions across the double bond, and an aryl alkyl ether, which provides stability under mild conditions but allows cleavage in acidic or oxidative environments. The 2D structure can be represented as a benzene ring with the methoxy and propenyl groups para to each other, while the 3D conformation is largely planar due to conjugation, with sp²-hybridized carbon atoms at the double bond exhibiting bond angles of approximately 120°. This planarity and the double bond's role in isomerism are central to anethole's stereochemical diversity and its distinction from related compounds like estragole.[6]
Physical and Spectroscopic Characteristics
Anethole exists as a colorless to pale yellow liquid at room temperature, exhibiting a characteristic sweet, licorice-like odor reminiscent of anise.[7] The trans-isomer, which predominates in natural and commercial sources, has a melting point of approximately 21 °C, a boiling point of 234–237 °C at atmospheric pressure, and a density of 0.988 g/cm³ at 25 °C.[1][9]Anethole demonstrates low solubility in water, approximately 0.11 g/L at 25 °C, rendering it practically insoluble under standard conditions, while it is highly soluble in ethanol (miscible at 1:8 in 80% alcohol), diethyl ether, chloroform, and fixed oils.[1][10] Its refractive index ranges from 1.559 to 1.562 at 20 °C, a property useful for purity assessment in analytical contexts.[1]A notable physical phenomenon associated with anethole is the Ouzo effect, also known as the louche effect, observed in anise-flavored alcoholic beverages such as ouzo, pastis, or absinthe. When these high-ethanol solutions (typically 40% v/v ethanol containing ~1% anethole) are diluted with water, the mixture turns milky or opalescent due to the spontaneous formation of a surfactant-free oil-in-water microemulsion. This occurs because water dilution reduces the ethanol concentration, decreasing anethole's solubility and leading to supersaturation; nanoscale droplets of anethole then nucleate homogeneously, grow via diffusion-limited coalescence, and stabilize as structured emulsions with ethanol-rich cores and anethole shells, requiring no external energy input.[11]Spectroscopic techniques provide key identifiers for anethole. In infrared (IR) spectroscopy, characteristic absorptions include a peak at ~1600 cm⁻¹ attributed to aromatic C=C stretching and ~1280 cm⁻¹ for the C-O stretch of the methoxy group.[12]Proton nuclear magnetic resonance (¹H NMR) in CDCl₃ reveals vinyl protons of the propenyl group around 6.3 ppm (multiplet for the =CH- protons). Ultraviolet-visible (UV-Vis) spectroscopy shows maximum absorption at 258 nm in isooctane, reflecting the conjugated π-system.[1]Mass spectrometry (electron ionization) displays the molecular ion at m/z 148 (M⁺), corresponding to its formula weight, with fragmentation patterns aiding isomer distinction.[13]
Sources and Production
Natural Occurrence
Anethole is primarily found in the essential oils of several aromatic plants, where it serves as a major volatile compound. In anise (Pimpinella anisum) seed oil, trans-anethole constitutes up to 90% of the total oil content. Fennel (Foeniculum vulgare) seed oil contains 50–80% anethole, predominantly in the trans configuration. Star anise (Illicium verum) fruit oil is rich in anethole, with concentrations ranging from 80–90%. These plants, belonging to the Apiaceae family except for star anise (Schisandraceae), accumulate high levels of anethole in their mature seeds and fruits, contributing to their characteristic licorice-like aroma.Anethole also occurs in lower concentrations in other botanicals, such as basil (Ocimum basilicum), bay laurel (Laurus nobilis), and certain magnolia species (Magnolia spp.), as well as in Clausena heptaphylla. In these minor sources, anethole levels typically range from trace amounts to several percent of the essential oil, varying by plant part and environmental conditions.The biosynthetic pathway of anethole in plants derives from phenylalanine through the phenylpropanoid metabolism. It begins with the deamination of phenylalanine by phenylalanine ammonia-lyase (PAL) to form cinnamic acid, followed by subsequent hydroxylation, methylation, and reduction steps involving specific enzymes like t-anol/isoeugenol synthase in Apiaceae species. This pathway is particularly active in the Apiaceae family, leading to the production of trans-anethole as the dominant isomer in mature plant tissues.In plants, anethole functions as a defensecompound, exhibiting toxicity against herbivores such as aphids and antifungal activity against plant pathogenic fungi like Aspergillus flavus. These properties help protect the host plant from biotic stresses, underscoring its evolutionary role in ecological interactions.
Industrial Synthesis
The primary industrial synthesis of anethole relies on the isomerization of estragole (4-allylanisole or methyl chavicol), which is often sourced from by-products such as sulfate turpentine derived from southern pine processing or essential oils like basil oil.[14] This process typically involves base-catalyzed isomerization using sodium or potassium hydroxide in ethanol at elevated temperatures, converting the allyl double bond to the conjugated propenyl position and favoring the thermodynamically stable trans-anethole isomer.[15] Industrial-scale operations, such as those pioneered by Glidden in the mid-20th century, distill crude mixtures from turpentine still bottoms containing estragole and anethole precursors, followed by selective isomerization and purification to yield predominantly trans-anethole.[14]An alternative classical route involves the acid-catalyzed condensation of anisole with propionaldehyde, forming 4-methoxypropiophenone as an intermediate, which is then reduced and dehydrated to anethole; this method, detailed in early patents, provides a petrochemical-based pathway independent of natural feedstocks.[16]Other synthetic methods include the Wittig olefination of p-anisaldehyde (4-methoxybenzaldehyde) with ethylidenetriphenylphosphorane (Ph₃P=CHCH₃), which directly forms the trans-propenyl linkage with high stereoselectivity under mild conditions, suitable for laboratory-scale but adaptable to production with phosphorus recycling.[17] Catalytic hydrogenation routes from anisaldehyde derivatives have been explored but are not predominant, as they typically require multi-step reduction-dehydration sequences to avoid over-reduction to saturated products. Emerging green alternatives employ biocatalysts, such as ruthenium complexes or enzymatic systems, for selective estragole isomerization in aqueous or solvent-free media, achieving high efficiency with reduced environmental impact.[18] Microwave-assisted processes further enhance these by accelerating isomerization rates while minimizing energy use, often integrating with hydrotalcite catalysts for near-quantitative conversion.[19]Industrial processes routinely achieve yields exceeding 95% for the trans-anethole isomer, with purification via distillation or crystallization ensuring pharmaceutical-grade purity (e.g., melting point 20–21°C and <3% cis content).[14] Historically, anethole production evolved from early 20th-century extractions of pine needle oils and turpentine fractions, where alpha-pinene-rich distillates were cracked and isomerized to phenylpropenoids, laying the groundwork for modern sulfate turpentine recovery in the U.S. pulp industry.[14]Economically, synthetic anethole from these routes is significantly cheaper than natural isolates for bulk production, with 1990s market prices for turpentine-derived material under $8–9 per pound compared to higher costs for essential oil extractions, though natural variants command premiums for organic certifications and perceived authenticity.[14] This cost advantage has sustained synthetic dominance, with global capacity from such processes meeting over 60% of demand in the late 20th century.[14]
Applications
Flavoring and Fragrance
Anethole serves as a primary flavoring agent in numerous food products, particularly those requiring an anise-like profile, such as licorice and aniseed candies. It is commonly incorporated into baked goods at average maximum levels of approximately 500 ppm, contributing a sweet, herbal note that enhances doughs, cookies, and pastries. In candies, usage reaches up to 530 ppm for soft varieties and 497 ppm for hard types, where it provides the signature licorice intensity without overpowering other ingredients.[20][21]In beverages, anethole imparts the distinctive anise character to spirits including absinthe, ouzo, pastis, sambuca, and raki, often at levels aligned with good manufacturing practices for GRAS substances. These drinks typically feature anethole concentrations derived from natural anise or fennel extracts, resulting in a balanced sweet-spicy profile when diluted with water, known as the ouzo effect. Historically, refractive index measurements were employed to detect adulteration in anise-flavored beverages like absinthe, where synthetic anethole additions could alter the optical properties compared to authentic botanical sources.[22][23][24]The sensory profile of anethole is characterized by anise-like, sweet, and herbal aroma descriptors, evoking licorice with warm, spicy undertones. Its high potency allows detection at low concentrations, making it effective even in trace amounts for flavor enhancement. Anethole holds GRAS status from the FDA and FEMA, supporting its safe application in these formulations at levels consistent with intended use.[25][26]In the fragrance sector, anethole is valued for its sweet-spicy notes in perfumes, soaps, and oral care products, where it adds depth to compositions mimicking natural anise or herbal accords. It frequently blends with vanillin for creamy vanilla-anise synergies or coumarin for enhanced warm, hay-like nuances, as seen in classic oriental and gourmand scents. These applications leverage anethole's stability and diffusive quality to create long-lasting, appealing aromatic profiles in personal care items.[27][28]
Chemical Precursor
Anethole functions as a versatile chemical precursor in industrial synthesis, enabling the production of various derivatives through targeted transformations. A primary reaction involves the base-catalyzed isomerization of cis-anethole to the thermodynamically favored trans-anethole, typically employing potassium hydroxide (KOH) in an alcoholic solvent at elevated temperatures, which proceeds with high selectivity and yields often exceeding 90% under optimized conditions.[29][30] This process is industrially significant for purifying and stabilizing anethole extracts from natural sources.Oxidation of anethole represents another key pathway, cleaving the propenyl side chain to yield anisaldehyde (4-methoxybenzaldehyde), a valuable intermediate in organic synthesis. This transformation is achieved using aqueous hydrogen peroxide with vanadium or iron-based catalysts, or molecular oxygen with metal-organic frameworks, attaining conversion yields of 80–90% in selective catalytic systems while minimizing over-oxidation to byproducts.[31][32] The resulting anisaldehyde serves as a building block for further chemical elaborations in legitimate industrial applications.Anethole also participates in cycloaddition reactions, notably as a dienophile in Diels-Alder processes, particularly radical cation-mediated variants initiated by photocatalysis or electrocatalysis. For instance, trans-anethole reacts with dienes such as isoprene to form substituted cyclohexene derivatives, enabling the construction of complex cyclic structures with yields up to 99% in single-chain polymer-confined systems.[33][34] These adducts are useful intermediates for pharmaceuticals and materials.In polymer chemistry, anethole acts as a bio-based starting material for synthesizing fluorinated monomers, such as benzocyclobutene derivatives, which polymerize into low-dielectric-constant (low-k) materials for high-frequency electronics, demonstrating its role in sustainable resin and polymer production.[35] While primarily employed in these legitimate pathways, anethole's structural features have raised regulatory concerns regarding its potential misuse as a precursor in unauthorized syntheses.[36]
Medicinal and Pharmaceutical Uses
Anethole, the primary active component in anise and fennel essential oils, is utilized as an expectorant in traditional herbal medicinal products for relieving cough associated with colds and bronchitis.[37] These oils, containing 80–95% trans-anethole, facilitate mucus clearance and ease respiratory symptoms through their mucolytic and spasmolytic effects, often incorporated into cough mixtures, particularly for pediatric use.[38] Anise essential oil, rich in trans-anethole (up to 72%), is specifically employed in expectorant formulations to support bronchial health in herbal remedies.[39]In digestive applications, anethole contributes to carminative preparations that alleviate bloating and gastrointestinal discomfort via its antispasmodic properties, commonly formulated as anise oil capsules or teas.[40] These products help settle the digestive tract by reducing gas and promoting smooth muscle relaxation in the stomach and intestines.[38] Supporting its efficacy, anethole's mild antimicrobial effects target gut pathogens, enhancing overall digestive aid in combination with other essential oils like fennel.[41]For oral care, anethole serves as an antibacterial additive in mouthwashes and toothpastes, combating periodontal bacteria such as Eikenella corrodens and Prevotella species due to its potent antimicrobial activity.[41] Anise-based mouth rinses containing anethole demonstrate efficacy comparable to chlorhexidine in reducing gingival bleeding and promoting oral healing.[41]Typical dosage guidelines for anethole-rich supplements, such as anise oil, recommend 50–200 μL three times daily for adults, equating to approximately 0.05–0.2 g of oil per day, often combined with other essential oils for enhanced therapeutic effects; use should not exceed 2 weeks without medical supervision.[37] These doses are derived from traditional herbal monographs and are contraindicated for children under 18 or those hypersensitive to anethole.[38]
Biological Activities
Antimicrobial and Antifungal Effects
Anethole displays notable antibacterial activity, particularly against Gram-positive pathogens such as Staphylococcus aureus, where minimum inhibitory concentrations (MIC) typically range from 0.1 to 0.5 mg/mL, achieved through disruption of the bacterial cell membrane integrity and leakage of intracellular contents.[42] This compound also inhibits Gram-negative bacteria, including Escherichia coli and Salmonella species, albeit at slightly higher concentrations, by altering membrane fluidity and permeability.[43] In vitro studies confirm these effects, highlighting anethole's potential as a natural antibacterial agent in combating foodborne and clinical pathogens.[43]The antifungal properties of anethole target opportunistic fungi like Candida albicans and Aspergillus species.[44] This activity stems from interference with ergosterol biosynthesis, a critical component of fungal cell membranes, leading to impaired membrane function and fungal cell death.[45] Evidence from in vitro assays supports its efficacy against these fungi, positioning anethole as a candidate for managing fungal infections in both clinical and agricultural contexts, often through synergistic interactions.[46]Key mechanisms underlying anethole's antimicrobial action include the induction of reactive oxygen species (ROS) generation, which causes oxidative damage to microbial cells, alongside inhibition of efflux pumps that expel antimicrobial agents.[46] Additionally, it disrupts biofilm formation by interfering with bacterial adhesion and extracellular matrix production, reducing the persistence of pathogens in chronic infections.[47] Anethole exhibits synergistic interactions with conventional antibiotics, such as vancomycin, lowering their required MIC and potentially mitigating resistance development in S. aureus strains.[48]In food preservation applications, anethole at concentrations of 0.1% effectively extends the shelf life of perishable items like dairy products and meats by suppressing microbial proliferation and oxidative spoilage.[49] Studies demonstrate reduced bacterial counts and prolonged freshness in treated samples, underscoring its role as a natural alternative to synthetic preservatives without compromising sensory qualities.[50]
Insecticidal and Repellent Properties
Anethole demonstrates notable insecticidal activity against several insect species, particularly mosquitoes such as Aedes aegypti and flies like the house fly (Musca domestica). Studies have reported an LD50 of approximately 75 μg/insect for trans-anethole via contact application against house fly adults, consistent with values for anethole-rich extracts.[51] For A. aegypti larvae, the LC50 value is approximately 29.3 μg/mL, indicating moderate to high toxicity in aquatic stages.[52] This toxicity arises from anethole's interference with key neurological targets in insects, including inhibition of acetylcholinesterase (AChE) enzyme activity and disruption of γ-aminobutyric acid (GABA) receptors, leading to overstimulation of the nervous system and eventual paralysis or death.[53][54]In addition to direct lethality, anethole acts through contact toxicity by penetrating the insect cuticle due to its lipophilic nature, allowing rapid absorption into the hemolymph and subsequent distribution to target organs.[55] It also exhibits fumigant effects, vaporizing to affect stored-product pests such as the red flour beetle (Tribolium castaneum) and rusty grain beetle (Cryptolestes ferrugineus), where exposure reduces AChE activity by up to 64% within 24 hours.[56] These properties make anethole suitable for integrated pest management in stored grains, with LC50 values for fumigation often in the range of 10–50 μL/L air against such species.[57]As a repellent, trans-anethole offers strong protection against mosquito vectors, achieving 100% repellency against A. aegypti at concentrations exceeding 0.988 mg/mL in laboratory assays, comparable to but shorter-lasting than DEET.[58] It is incorporated into natural larvicides, where formulations at 5–10% concentration provide oviposition deterrence and larval mortality rates of 80–95% against Aedes and Anopheles species, the latter being key malaria vectors.[59] The repellency likely stems from binding to odorant-binding proteins (OBPs) like AaegOBP1, interfering with host-seeking olfaction.[60]Anethole's environmental applications emphasize its role in eco-friendly pesticides, reducing reliance on synthetic chemicals through biodegradable formulations. It shows synergistic effects with eugenol, enhancing toxicity against pests like termites (Reticulitermes speratus) where binary mixtures lower LD50 values by 2–5 fold compared to individual compounds, promoting broader-spectrum control with minimal environmental residue.[61]
Estrogenic and Hormonal Effects
Anethole exhibits estrogenic activity primarily through its ability to bind to estrogen receptor alpha (ERα), functioning as a phytoestrogen that mimics endogenous estrogens. In vitro studies using the yeast estrogen screen (YES) assay have demonstrated that trans-anethole induces estrogenic responses at high concentrations, with EC50 values ranging from 45 to 650 µg/mL and relative potencies of 8.3 × 10⁻⁸ to 1.2 × 10⁻⁶ compared to 17β-estradiol.[62] Molecular docking analyses indicate that anethole interacts with both ERα and ERβ isoforms, forming hydrogen bonds and hydrophobic interactions similar to those observed with genistein, another phytoestrogen, though with weaker overall affinity.[63]In vivo, administration of (E)-anethole at 80 mg/kg/day for 3 days to immature female rats significantly increases uterine weight, indicative of estrogenic stimulation leading to uterine hyperplasia.[64]Anethole also influences prolactin secretion by acting as a dopamine receptor antagonist, thereby blocking dopamine's inhibitory effect on prolactin release from the pituitary gland. This mechanism structurally resembles dopamine, allowing competitive binding at receptor sites and elevating serum prolactin levels in animal models.[65] Consequently, anethole has been investigated as a galactagogue, with studies showing enhanced milk production in lactating models due to sustained prolactin elevation.[66]The phytoestrogenic properties of anethole involve receptor-mediated mimicry without genotoxic effects, as confirmed by evaluations from the Joint FAO/WHO Expert Committee on Food Additives, which classify it as non-genotoxic based on multiple in vitro and in vivo assays.[67] In male models, anethole modulates testosterone levels, with dietary supplementation of anethole-rich fennel seed powder increasing serum testosterone concentrations in sheep, suggesting differential endocrine effects across sexes.[68]Research on anethole's hormonal effects highlights its potential applications in hormone replacement therapy for menopausal symptoms, given its mild estrogenic profile, but raises concerns regarding endocrine disruption at higher doses, particularly in sensitive populations.[69]
Neurological and Anticancer Effects
Anethole exhibits anxiolytic and antidepressant effects in preclinical models, primarily through modulation of the GABAergic system and serotonin pathways via inhibition of monoamine oxidase-A (MAO-A), which enhances monoamine levels and reduces oxidative stress.[70] In a mouse model of maternal separation stress, anethole administered at 10 mg/kg intraperitoneally reduced immobility time in the forced swimming test and increased grooming in the splash test, while decreasing hippocampal malondialdehyde levels and nitrite concentrations to alleviate depressive-like behaviors.[71] These effects highlight anethole's potential to mitigate early-life stress-induced neurochemical imbalances.[70]Anethole demonstrates neuroprotective properties against oxidative stress and neuroinflammation in models of Alzheimer's disease-like pathology. In scopolamine-induced cognitive impairment in rats, anethole reversed memory deficits by enhancing antioxidant capacity, reducing malondialdehyde, and suppressing inflammatory markers such as tumor necrosis factor-alpha.[72] It also improves long-term potentiation and synaptic function while lowering neuroinflammatory responses in amyloid-beta-exposed neuronal cultures.[70]In terms of anticonvulsant activity, anethole delays seizure onset in pentylenetetrazol (PTZ)-induced models through GABAergic modulation and potential regulation of calcium and potassium channels. At 400 mg/kg intraperitoneally in mice, it significantly prolonged latency to myoclonic jerks and tonic-clonic seizures compared to PTZ alone.[73] For antinociceptive effects, anethole increases paw withdrawal latency in the hot-plate test, indicating central analgesic activity mediated by reduced oxidative stress and enhanced superoxide dismutase and glutathione peroxidase levels.[70]Anethole shows anticancer potential by inducing apoptosis in oral squamous cell carcinoma cells through caspase-3 activation and poly(ADP-ribose) polymerase cleavage, with an IC50 of approximately 50 μM in Ca9-22 cells.[74] It exerts anti-proliferative effects in breast cancer cells (MCF-7 and MDA-MB-231) by inhibiting NF-κB transcriptional activity, independent of estrogen receptor status, leading to reduced cell survival and colony formation.[75] Similar NF-κB suppression contributes to its growth-inhibitory actions in colon cancer models, where it disrupts pro-survival signaling and promotes cell cycle arrest.[76] Anethole enhances the efficacy of doxorubicin in triple-negative breast cancer cells by promoting reactive oxygen species-mediated apoptosis, mitochondrial membrane potential loss, and endoplasmic reticulum stress, resulting in synergistic cytotoxicity at sub-toxic doses.[77]Recent preclinical studies from 2024–2025 reinforce these findings: the maternal separation model confirmed antidepressant benefits at low doses via oxidative stress modulation, while combinations with chemotherapeutics showed promise against oral cancer through NF-κB and MAPK pathway inhibition; 2025 research further highlights synergistic insecticidal applications and comprehensive neurological benefits including memory enhancement.[71][76][78] In doxorubicin-treated models, low-dose anethole provided renal protection by attenuating oxidative damage, though high doses induced mild, reversible nephrotoxicity.[79] These effects may partially overlap with anethole's estrogenic activity in hormone-sensitive cancers.[76]
Safety and Toxicology
Toxicity Profile
Anethole exhibits low acute toxicity via oral administration, with an LD50 value of 2,090 mg/kg in rats. Dermal exposure also demonstrates low toxicity, with an LD50 exceeding 4,900 mg/kg in rabbits.[80] However, it has potential for skin sensitization, causing allergic contact dermatitis in approximately 5% of patch-tested patients based on testing with related essential oils containing high anethole concentrations.[81]In chronic exposure studies, anethole induces hepatotoxicity in rodents at doses greater than 500 mg/kg/day, particularly in female rats where intakes around 550 mg/kg/day led to liver enzyme induction and histopathological changes.[26] It shows no evidence of carcinogenicity to humans, supported by long-term feeding studies in rats revealing a low incidence of liver tumors in female rats at doses up to 550 mg/kg/day, though these effects are considered not relevant due to efficient metabolic detoxification at typical humanexposure levels.[26] Reproductive effects occur via its estrogenic activity at high doses, such as 50–80 mg/kg in rats, resulting in anti-fertility outcomes like disrupted implantation due to hormonal imbalance.[82]Exposure risks include potential aspiration hazard upon inhalation, as vapors may cause respiratory irritation or coughing in high concentrations, though overall inhalation toxicity is low with an LC50 of ≥5.1 mg/L over 4 hours in rats. Genotoxicity tests are negative, with trans-anethole failing to induce mutations in the Ames Salmonella/microsome assay across multiple strains.[83]Anethole undergoes rapid hepatic metabolism primarily through cytochrome P450-mediated oxidation to intermediates like anethole epoxide and p-hydroxyphenylpropene, followed by phase II conjugation with glucuronic acid to form excretable glucuronides. Its plasma half-life is short, approximately 1–2 hours, reflecting efficient elimination via urine and bile in rodents.[84] As a chemical precursor in illicit drug synthesis, such as for amphetamines, misuse concerns may amplify exposure risks beyond typical flavoring uses.[26]
Regulatory Status and Concerns
Anethole, specifically trans-anethole, has been affirmed as generally recognized as safe (GRAS) by the Flavor and Extract Manufacturers Association (FEMA) for use as a direct food additive since 1965, a status recognized by the U.S. Food and Drug Administration (FDA) for flavoring purposes in baked goods, candies, ice creams, and beverages.[85] In 2022, the U.S. Environmental Protection Agency (EPA) established an exemption from pesticide residue tolerances for trans-anethole when used as an inert ingredient in pesticide formulations applied to food crops, provided it does not exceed 3% of the final formulation.[86] No specific maximum levels are mandated for its use as a flavoring in foods under GRAS, but concentrations are typically low to align with good manufacturing practices.In the European Union, the European Chemicals Agency (ECHA) has not established a harmonized classification for carcinogenicity of anethole, but it is classified as a skin sensitizer (Skin Sens. 1) due to its potential to cause allergic reactions.[87] Anethole is permitted in cosmetic products as a fragrance ingredient under Regulation (EC) No 1223/2009, subject to labeling requirements for allergens when concentrations exceed 0.001% in leave-on products or 0.01% in rinse-off products; no upper concentration limit of 0.5% is explicitly harmonized, though industry standards like those from the International Fragrance Association (IFRA) recommend restrictions based on sensitization data.[88]Due to its potential conversion to illicit substances such as para-methoxyamphetamine (PMA), a structural analog of MDMA, via intermediates like those related to isosafrole, anethole is subject to regulatory controls as a precursor in some jurisdictions. In Australia, it falls under precursor monitoring provisions in drug misuse laws, requiring permits for import and use to prevent diversion, akin to Schedule 4 controls for related chemicals.[89] In the United States, the Drug Enforcement Administration (DEA) monitors anethole as a potential pre-precursor for amphetamine synthesis in clandestine labs, though it is not listed as a controlled substance itself.[90]The World Health Organization (WHO), through the Joint FAO/WHO Expert Committee on Food Additives (JECFA), has established an acceptable daily intake (ADI) for trans-anethole of 0–1.25 mg/kg body weight, based on its low toxicity profile.[91] For veterinary use, the European Food Safety Authority (EFSA) has assessed anethole as safe in animal feed up to maximum levels such as 25 mg/kg complete feed for avianspecies and 125 mg/kg for pigs, with no withdrawal period required, though higher levels may pose risks to target animals or residues in products.[92]
History
Traditional and Early Uses
Anethole-containing plants, particularly anise (Pimpinellaanisum), have been employed in ancient Egyptian medicine since around 1550 BCE, as documented in the Ebers Papyrus, where anise served as a carminative to relieve flatulence and as a mouthwash for oral health.[93] In ancient Greece, Hippocrates recommended anise around 400 BCE for digestive disorders and as an aphrodisiac to enhance libido, while Pythagoras and Pliny the Elder also praised its stomachic properties for alleviating indigestion.[94] Romans incorporated anise into spiced wines and cakes, such as mustacei—grape must-based pastries flavored with anise and cumin—creating precursors to anise-infused beverages that aided digestion during meals.[95]Culinary applications of these plants trace back to Middle Eastern regions around 1500 BCE, where anise seeds flavored breads, stews, and confections, as evidenced in early trade records and archaeological findings of spice use in the Levant.[96] In Indian cuisines, closely related fennel seeds (Foeniculum vulgare), rich in anethole, have been used since ancient times, with cultivation dating back to at least 2000 BCE and references in early Ayurvedic texts as a spice for curries and digestive aids, integrating into daily meals for their aromatic and carminative qualities.[97] By medieval Europe, anise flavored liqueurs and cordials, such as those macerated in wine for distillation, became common by the 14th century in Italy and France, often served as digestifs to settle the stomach after feasts.[98]Medicinal traditions worldwide highlight anethole's role in addressing respiratory and gastrointestinal ailments. In Ayurveda, fennel seeds have been used since ancient times to expel excess kapha dosha, relieving coughs, breathing difficulties, and digestive issues like bloating and indigestion.[99] Traditional Chinese medicine employs star anise (Illicium verum) to warm the body, support lung function against colds and flu, and ease digestive discomfort such as nausea and abdominal pain.[100] Native American communities, including the Cherokee, traditionally administered fennel infusions to infants for colic relief, reducing flatulence and gastrointestinal spasms.[101]Beyond practical uses, anethole-rich plants held cultural symbolism in folklore, often burned or carried as amulets to ward off evil spirits and protect against malevolent forces, a practice rooted in ancient Mediterranean and Middle Eastern beliefs.[102]
Isolation and Commercial Development
Anethole was first isolated from anise oil in the mid-19th century through fractional distillation techniques developed in the early 1800s. The compound, initially known as "anise camphor," was named and structurally characterized by Germanchemist Emil Erlenmeyer in 1866, who proposed its molecular formula as C10H12O based on degradation studies and comparative analysis with related anisole derivatives. Although Erlenmeyer proposed the correct structure in 1866, it was confirmed in 1872 through further synthesis and analysis.[3] Earlier contributions included Swisschemist Nicolas-Théodore de Saussure's initial chemical investigation in 1820 and French chemist Jean Baptiste Dumas's determination of the empirical formula in 1832 via combustion analysis.[103]Commercial development of anethole accelerated in the 20th century with the rise of synthetic production methods, particularly following World War II, as the flavor and fragrance industry expanded to meet growing demand for consistent, cost-effective ingredients in food, beverages, and pharmaceuticals. Synthetic routes, such as the condensation of anisole with propionaldehyde followed by dehydration, became prominent, enabling large-scale production beyond natural extraction from anise, fennel, and star anise oils. By the 1950s, anethole had become a staple in the flavor sector, with its safety for use affirmed when trans-anethole was listed as generally recognized as safe (GRAS) by the Flavor and Extract Manufacturers Association (FEMA) in 1965.[104] This GRAS status was reaffirmed in 1997 after comprehensive toxicological reviews confirmed low intake levels and efficient metabolic detoxification.[26]Patent activity marked key innovations in anethole production and application. Early 20th-century patents focused on isomerization processes to convert cis-anethole to the more stable and desirable trans isomer, improving yield and purity for industrial use; for instance, methods involving acidic catalysis were documented around 1910. In recent decades, patents have addressed advanced formulations, such as nanoemulsions incorporating anethole for enhanced solubility and stability in clear beverages, reflecting ongoing efforts to optimize delivery in low-alcohol and non-alcoholic drinks. Amid rising consumer preference for natural ingredients in the 2020s, the industry has shifted toward sustainable sourcing from renewable plant materials, reducing reliance on synthetic alternatives while maintaining supply for the expanding natural flavors market.[105]