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Campesterol

Campesterol is a phytosterol, a class of plant-derived sterols with the molecular formula C₂₈H₄₈O, featuring a tetracyclic steroid skeleton identical to cholesterol but distinguished by an additional methyl group at the C-24 position of its side chain.^[1] This compound is ubiquitous in the plant kingdom, where it constitutes a primary sterol in cell membranes, regulating fluidity, permeability, and the formation of lipid rafts essential for cellular signaling and structural integrity.^[2] In plant biosynthesis, campesterol is derived from cycloartenol through methylation steps catalyzed by sterol C-24-methyltransferase^[3] and serves as a critical precursor to brassinosteroids, a family of phytohormones that govern processes such as cell elongation, vascular differentiation, and stress responses.^[4] In human physiology, campesterol is ingested through plant-based foods including vegetable oils (comprising 0.1–1.0% of their content), grains, nuts, seeds, legumes, fruits, and green/yellow vegetables, yielding a typical daily intake of 200–300 mg in Western diets.^[1] With an absorption rate of less than 5% in the small intestine—higher than that of β-sitosterol (0.5%) but far below cholesterol (15–80%)—it competitively inhibits cholesterol uptake by intestinal micelles, leading to reduced serum LDL and VLDL levels when consumed at 150–400 mg per day, thereby supporting cardiovascular health.^[1]^[5] Preclinical studies further indicate antidiabetic potential, as its derivative 5-campestenone has demonstrated blood glucose-lowering and insulin-sensitizing effects in rodent models of type 2 diabetes, alongside anticarcinogenic properties through enhanced apoptosis in colon cancer cells.^[5] However, excessive intake may increase colorectal cancer risk and impair absorption of fat-soluble vitamins like β-carotene and vitamin D.^[1]

Chemical Properties

Structure and Nomenclature

Campesterol is a phytosterol characterized by the molecular formula C_{28}H_{48}O. It features a tetracyclic steroid backbone typical of sterols, with a hydroxyl group attached at the 3β position of the A ring, a double bond between carbons 5 and 6, and an eight-carbon side chain at carbon 17 that includes a methyl substituent at carbon 24.^[6] The systematic IUPAC name for campesterol is (3S,8S,9S,10R,13R,14S,17R)-17-[(2R,5R)-5,6-dimethylheptan-2-yl]-10,13-dimethyl-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1H-cyclopentaphenanthren-3-ol, though it is commonly referred to as campest-5-en-3β-ol in biochemical literature.^[6] This nomenclature reflects its classification within the ergostane series of sterols, derived from the parent hydrocarbon ergostane.^[7] Compared to cholesterol (C_{27}H_{46}O), campesterol exhibits a key structural modification in the form of an additional methyl group at carbon 24 of the side chain, while retaining the identical core structure including the 3β-hydroxyl and Δ⁵ double bond.^[6] This alteration increases the hydrophobicity of the side chain and distinguishes campesterol as a plant-derived analog. The predominant natural isomer is β-campesterol, defined by the 3β-hydroxyl configuration; the α-isomer, with a 3α-hydroxyl, occurs rarely in biological systems.^[2] Campesterol shares close structural similarity with other major phytosterols, such as β-sitosterol and stigmasterol, all of which possess the same steroid nucleus but differ in side chain substitutions at carbon 24. β-Sitosterol features an ethyl group at C24, while stigmasterol includes an ethyl at C24 along with a trans double bond between C22 and C23, enabling functional variations in plant membranes.^[8]

Physical and Chemical Characteristics

Campesterol is a white crystalline solid at room temperature.^[9] Its melting point ranges from 156 to 160 °C, reflecting the thermal stability of its steroidal structure under moderate heating conditions.^[9] Campesterol exhibits low solubility in water, approximately 0.00015 mg/L at 25 °C, which limits its direct dissolution in aqueous environments. In contrast, it shows high solubility in organic solvents, such as chloroform at 20 mg/mL and ethanol at about 9 mg/mL, facilitating its extraction and analysis in non-polar media.^[10] The compound is chemically stable under neutral pH and normal storage conditions but is susceptible to oxidation when exposed to air, particularly during heating processes above 180 °C, leading to the formation of oxidation products. Spectroscopically, campesterol displays ultraviolet absorption at around 204-210 nm, attributable to its Δ⁵ double bond in the sterol ring system.^[11] In ¹H NMR analysis (typically in CDCl₃), characteristic signals for methyl groups include singlets near δ 0.68 (C-18) and δ 1.02 (C-19), doublets at δ 0.82 (C-26/27) and δ 0.93 (C-21), aiding in structural identification.^[12]^[13]

Biosynthesis and Natural Occurrence

Biosynthetic Pathway

Campesterol biosynthesis in plants occurs primarily through the mevalonate pathway, which begins with the condensation of three molecules of acetyl-CoA to form 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA), followed by reduction to mevalonate and subsequent phosphorylation and decarboxylation steps to yield isopentenyl pyrophosphate (IPP). IPP isomerizes to dimethylallyl pyrophosphate (DMAPP) and condenses to form geranyl pyrophosphate (GPP), then farnesyl pyrophosphate (FPP), which dimerizes to presqualene pyrophosphate and is converted to squalene by squalene synthase. Squalene is oxidized to 2,3-oxidosqualene, setting the stage for cyclization into the sterol backbone.^[14] The core sterol synthesis diverges in plants via the cycloartenol pathway, where 2,3-oxidosqualene is cyclized to cycloartenol by cycloartenol synthase (CAS), a plant-specific enzyme encoded by the CAS1 gene in Arabidopsis thaliana. Cycloartenol undergoes demethylation at C-4 and C-14 positions; the C-14 demethylation is catalyzed by sterol C-14 demethylase (CYP51), removing the 14α-methyl group to form intermediates like obtusifoliol. A key plant-specific step involves methylation at the C-24 position of the side chain by sterol methyltransferase 1 (SMT1), converting cycloartenol to 24-methylene cycloartanol. Further modifications, including additional demethylations and isomerizations, lead to 24-methylene lophenol, which is then reduced at the Δ24 double bond by Δ24-sterol reductase (SSR1 or DWF5 homologs) to produce campesterol. Although a minor lanosterol pathway exists in some plants, catalyzed by lanosterol synthase (LAS1), it contributes less than 5% to campesterol formation in species like Arabidopsis.^[15]^[16]^[14] The pathway is regulated by plant developmental stages, with sterol levels varying across tissues such as roots, leaves, and seeds to support processes like embryogenesis and cell elongation; for instance, campesterol accumulation peaks during rapid growth phases. Environmental factors, including light exposure, modulate enzyme expression and flux, as light signaling influences HMG-CoA reductase activity and overall sterol homeostasis to adapt membrane fluidity. Unlike animal cholesterol biosynthesis, which proceeds solely from lanosterol without C-24 alkylation and yields unmethylated cholesterol, plant pathways incorporate SMT-mediated methylation, enabling the production of C-28 sterols like campesterol essential for phytosterol diversity.^[14]^[16]

Dietary Sources

Campesterol is the second most abundant phytosterol in plants after β-sitosterol, typically comprising 15-20% of the total phytosterol content across various plant sources.^[17]^[18] Primary dietary sources of campesterol are plant-based foods rich in lipids, with vegetable oils serving as the richest natural contributors. Rapeseed (canola) oil contains 241-268 mg of campesterol per 100 g, while corn oil provides approximately 197-215 mg per 100 g, and soybean oil around 56-63 mg per 100 g.^[19]^[20] Nuts and seeds also contribute, though at lower levels; for instance, sesame seeds contain about 50 mg per 100 g, and almonds roughly 5 mg per 100 g. Grains, particularly wheat germ, offer notable amounts, with total phytosterols reaching 400 mg per 100 g, of which campesterol constitutes a significant portion estimated at 60-80 mg per 100 g based on typical phytosterol profiles in cereals. Fruits and vegetables provide smaller quantities; navel oranges, for example, contain total phytosterols up to 33 mg per 100 g, with campesterol around 5-7 mg per 100 g.^[21]^[20]^[22] Processed foods fortified with phytosterols, such as margarines and dairy alternatives, can deliver higher doses, often containing up to 2 g of total phytosterols per serving, with campesterol esters accounting for about 25% of the mix.^[23] In a typical Western diet, daily campesterol intake ranges from 30-80 mg, contributing to overall phytosterol consumption of 150-400 mg, though vegetarian diets may provide higher levels due to increased plant food intake.^[24]^[25] Commercially, campesterol is extracted from sources like wheat germ oil and tall oil for use in fortified products.^[26]^[27]

Biological Roles

In Plants

Campesterol serves as a key structural component in plant cell membranes, where it integrates into phospholipid bilayers to modulate membrane fluidity and permeability, exerting a strong ordering effect similar to cholesterol in animal cells.^[28] This role helps maintain membrane homeostasis, ensuring proper function of membrane-bound proteins and overall cellular integrity under varying environmental conditions.^[29] In plant plasma membranes, campesterol constitutes a significant portion of total sterols, contributing to the formation of ordered lipid domains that support signal transduction and transport processes.^[30] The compound plays an essential role in plant growth and development, particularly in root elongation and pollen tube growth, where it influences cell expansion and reproductive processes.^[31] For instance, alterations in campesterol levels affect polar auxin transport, which is critical for gravitropism and directional root growth.^[32] Additionally, campesterol contributes to stress responses, such as enhanced drought tolerance, by stabilizing sterol-rich lipid rafts that facilitate adaptive signaling and membrane repair during water deficit.^[29] As a precursor in brassinosteroid (BR) biosynthetic pathways, campesterol is converted to active hormones that regulate gene expression involved in developmental processes, including cell division and elongation.^[33] These BRs, derived from campesterol, activate transcription factors like BES1, which promote the expression of growth-related genes and integrate environmental cues into developmental programs.^[34] Plants with deficiencies in campesterol, often observed in mutants disrupted in sterol biosynthesis genes like DWF1, exhibit altered sterol profiles with reduced campesterol and elevated precursors, leading to impaired growth phenotypes such as dwarfism and reduced fertility.^[35] These mutants display pleiotropic defects, including shortened roots and abnormal embryo development, underscoring campesterol's necessity for balanced sterol composition and normal morphogenesis.^[36] From an evolutionary perspective, the prevalence of campesterol in plant membranes represents an adaptation to terrestrial environments, where its ethyl-substituted structure enhances membrane cohesion and fluidity under fluctuating temperatures and stresses, distinguishing plant sterol profiles from those in other eukaryotes.^[37] This fine-tuned sterol composition likely evolved to support the unique demands of plant cell walls and signaling networks.^[38]

In Animals and Humans

In animals and humans, campesterol is obtained exclusively from dietary sources, as mammals lack the complete biosynthetic pathway present in plants to produce this phytosterol de novo.^[24] Following ingestion, campesterol exhibits poor intestinal absorption, with bioavailability typically ranging from 2% to 10%, lower than that of cholesterol (40-60%).^[39] It is solubilized and transported across the unstirred water layer of the intestinal lumen via mixed micelles composed of bile acids, monoglycerides, and phospholipids, facilitating uptake into enterocytes alongside cholesterol.^[40] This micellar incorporation allows limited passage through the brush border membrane, primarily in the proximal small intestine.^[5] Once absorbed, campesterol enters the bloodstream via chylomicrons and is taken up by the liver, where it undergoes rapid metabolism and excretion. A portion of campesterol is converted to its saturated derivative, campestanol, through reduction by gut microbiota in the intestinal lumen or by hepatic enzymes such as steroid 5α-reductase.^[5]^[41] The majority of absorbed campesterol is not retained but is actively effluxed back into the intestinal lumen or secreted into bile via ATP-binding cassette transporters ABCG5 and ABCG8, promoting fecal elimination.^[42] Biliary secretion rates for campesterol average approximately 0.76 mg/h in healthy adults, contributing to its efficient clearance from the body.^[42] In circulation, serum campesterol levels are low, typically ranging from 0.2 to 0.5 mg/dL in unsupplemented individuals, reflecting its restricted absorption and high turnover; these concentrations can elevate to 0.6-1 mg/dL with dietary phytosterol supplementation (e.g., 1.6-3 g/day).^[43]^[5] At the cellular level, absorbed campesterol incorporates minimally into mammalian cell membranes due to its low plasma abundance, partially substituting for cholesterol and modulating membrane fluidity and permeability, though to a lesser extent than cholesterol itself.^[24] During intestinal uptake, campesterol competes with cholesterol for the Niemann-Pick C1-like 1 (NPC1L1) transporter on enterocytes, reducing overall cholesterol absorption efficiency.^[44]

Health Effects

Effects on Blood Lipids

Campesterol, a plant-derived phytosterol, exerts its primary influence on blood lipids by inhibiting intestinal cholesterol absorption. It competes with cholesterol for incorporation into mixed micelles in the intestinal lumen, thereby displacing cholesterol and reducing its solubility and availability for uptake by enterocytes.^[24] Additionally, campesterol competes with cholesterol for the Niemann-Pick C1-like 1 (NPC1L1) transporter on the apical surface of intestinal cells, further limiting cholesterol transport into the bloodstream.^[45] This mechanism mimics the action of pharmaceutical cholesterol absorption inhibitors like ezetimibe, which also target NPC1L1.^[46] Clinical evidence from randomized controlled trials (RCTs) and meta-analyses demonstrates that campesterol supplementation, typically as part of phytosterol mixtures, reduces low-density lipoprotein (LDL) cholesterol levels in a dose-dependent manner. At an intake of approximately 2 g per day, LDL cholesterol is lowered by 8-15%, with meta-analyses of over 100 RCTs confirming this effect across diverse populations, including those with hypercholesterolemia.^[47] For instance, a comprehensive meta-analysis reported an average 10% reduction in LDL cholesterol at this dose, with greater efficacy observed in individuals with higher baseline cholesterol levels.^[48] These reductions are sustained with chronic intake and contribute to improved lipid profiles without significant alterations in daily cholesterol synthesis rates. Regarding other lipid parameters, campesterol has minimal impact on high-density lipoprotein (HDL) cholesterol or triglycerides, though some studies note slight increases in HDL (up to 3-5%) in certain cohorts.^[49] It also lowers apolipoprotein B (apoB) levels by 5-10%, reflecting reduced atherogenic particle numbers, as apoB is a key marker of LDL particle concentration.^[50] When combined with statins, which primarily inhibit hepatic cholesterol synthesis, campesterol enhances LDL cholesterol reduction by an additional 5-10%, counteracting the potential increase in intestinal absorption induced by statin therapy.^[51] This synergy is supported by meta-analyses showing greater overall LDL lowering (up to 20% additive effect) in hypercholesterolemic patients on combined regimens.^[52] Serum campesterol levels serve as a reliable biomarker for cholesterol absorption efficiency, with higher concentrations correlating positively with the fractional absorption of dietary cholesterol (r ≈ 0.4-0.6 in population studies).^[53] Ratios of serum campesterol to cholesterol are particularly indicative, as they reflect individual variability in intestinal uptake and predict responsiveness to absorption inhibitors.^[54] This biomarker utility aids in personalizing lipid-lowering interventions.

Anti-inflammatory and Other Benefits

Campesterol exhibits notable anti-inflammatory effects, primarily through inhibition of the NF-κB signaling pathway and suppression of pro-inflammatory cytokine production. In preclinical studies using complete Freund's adjuvant-induced arthritic rat models, campesterol ester derivatives treatment at doses of 50–100 mg/kg significantly reduced paw edema volume by up to 50% over 28 days and alleviated joint inflammation, comparable to standard therapies like piroxicam. These outcomes were accompanied by downregulation of key inflammatory mediators, including TNF-α, IL-1β, and IL-6 mRNA expression, alongside upregulation of the anti-inflammatory cytokine IL-4.^[55] A 2023 systematic review of 15 in vivo studies underscored campesterol's therapeutic potential for rheumatoid arthritis, highlighting its ability to modulate immune responses and cytokine profiles to mitigate arthritic symptoms without notable toxicity. These anti-inflammatory actions extend to broader immune modulation, where campesterol shifts the T helper 1/Th2 balance toward anti-inflammatory responses and reduces proinflammatory markers like IL-6, IL-8, and TNF-α in various cellular and animal models.^[57]^[5] Beyond inflammation, campesterol's mechanisms involve potent antioxidant activity, acting as a free radical scavenger to neutralize reactive oxygen species and peroxides, thereby protecting cellular components from oxidative damage. This scavenging occurs via hydrogen transfer and radical adduct formation, particularly effective against medium-reactivity peroxyl radicals, and contributes to mitochondrial stabilization by enhancing membrane potential and ATP production. In immune contexts, these properties further support modulation of macrophage and lymphocyte phenotypes, fostering an anti-inflammatory milieu.^[58]^[5] Campesterol also shows anticancer potential, with epidemiological data linking higher dietary intake to reduced overall cancer risk; a meta-analysis of over 16,000 participants reported a linear dose-response association, where each 10 mg/day increment in campesterol consumption correlates with a 13% lower risk. In preclinical models, campesterol induces apoptosis in cancer cells by elevating pro-apoptotic proteins such as Bax, Bak, and cytochrome c, alongside promoting autophagy via BECN1 upregulation and reactive oxygen species generation. These effects, observed in cell lines, suggest applicability to prostate cancer, where phytosterol-rich diets (including campesterol) have been associated with inhibited tumor growth and lower incidence in population studies.^[59]^[60] Regarding prostate health, campesterol contributes to supportive effects as part of phytosterol mixtures in supplements like saw palmetto extracts, which have shown alleviation of benign prostatic hyperplasia symptoms in some clinical trials, likely through anti-inflammatory and antiproliferative actions. Neuroprotective benefits have been evidenced in Alzheimer's disease animal models, where esterified campesterol crosses the blood-brain barrier to decrease amyloid-β aggregation, inhibit β- and γ-secretase activity, and attenuate neuroinflammation via gut microbiota modulation and short-chain fatty acid enrichment. These interventions improve cognitive function and reduce microglial activation, positioning campesterol as a potential modulator of neurodegenerative processes.^[61]^[62]

Adverse Effects and Risks

High intakes of campesterol, typically as part of phytosterol supplementation exceeding 3 g/day, can interfere with the absorption of fat-soluble vitamins (A, D, E, K) and carotenoids in the intestine by competing for micellar solubilization, leading to reductions of approximately 7-16% in plasma concentrations of β-carotene, α-tocopherol, and other carotenoids, though levels generally remain within normal ranges and are not clinically significant at recommended doses.^[63] In individuals with sitosterolemia, a rare autosomal recessive genetic disorder caused by mutations in the ABCG5 or ABCG8 genes, excessive intestinal absorption of campesterol and other plant sterols occurs, resulting in elevated plasma levels often exceeding 10 mg/dL, which can manifest as tendon xanthomas, xanthelasmas, corneal arcus, and premature atherosclerosis due to sterol deposition in tissues.^[64] Beyond sitosterolemia, cardiovascular risks from campesterol are primarily observed in this genetic context, where it promotes atherosclerotic plaque formation analogous to cholesterol; however, rare reports and genetic studies suggest a potential modest increase in coronary artery disease risk with high phytosterol intake in the general population without genetic predisposition, though large meta-analyses find no overall association between serum campesterol levels and cardiovascular events.^[65]^[24] Other potential adverse effects include mild gastrointestinal disturbances such as diarrhea, nausea, indigestion, or constipation, reported occasionally at intakes above 2 g/day, while no significant endocrine disruption has been established in humans at typical dietary or supplemental levels.^[24] The European Food Safety Authority (EFSA) deems phytosterols, including campesterol, safe up to 3 g/day for the general population but recommends monitoring and caution in vulnerable groups such as those with sitosterolemia, short bowel syndrome, or high oxidative stress, to avoid potential accumulation of oxidation products.^[66]

Uses and Applications

In Food and Nutrition

Campesterol, as a component of phytosterol mixtures, is included in the European Union designation E499 for stigmasterol-rich plant sterols, which are authorized specifically as a stabilizer and ice nucleating agent in ready-to-freeze alcoholic cocktails at levels up to 800 mg/kg.^[67] General phytosterol mixtures containing campesterol are commonly incorporated into fat spreads, yogurts, and fruit juices, typically at levels of 0.8 to 2 grams per serving, to support qualified health claims related to cholesterol reduction when consumed as part of a balanced diet.^[68] For instance, fortified spreads provide about 1.6 to 2 grams of total phytosterols per daily recommended intake, while yogurt drinks and juices deliver 0.8 to 1.3 grams per serving, aligning with regulatory thresholds for efficacy. In November 2025, EFSA approved health claims for sunflower-derived phytosterols, further supporting their use in functional foods.^[69] Nutritional guidelines from the FDA and EFSA authorize heart health claims for foods containing 1.3 to 2 grams of total phytosterols per day, including campesterol, which contributes significantly to the mixture's cholesterol-lowering effects in functional foods.^[70] These claims state that adequate intake may reduce the risk of coronary heart disease by lowering LDL cholesterol levels, provided the products are low in saturated fat and cholesterol.^[71] Campesterol-enriched functional foods form a key segment of the global market, driven by consumer demand for heart-healthy options and supported by evidence from clinical trials showing 8-10% LDL reduction at these doses.^[72] To improve incorporation into food matrices, campesterol and other phytosterols are often esterified with fatty acids, enhancing their solubility in fat-based products like margarines and dairy spreads while maintaining stability during processing.^[24] This esterification process increases fat solubility by up to tenfold in edible oils, allowing effective delivery without altering food texture or taste.^[73] Dietary recommendations position campesterol-containing phytosterols as an adjunct for individuals with hypercholesterolemia, with 2 grams daily intake advised alongside statin therapy or lifestyle changes to optimize LDL reduction.^[48] However, efficacy may diminish in low-fat diets, as phytosterols require co-consumption with dietary fats for optimal intestinal absorption and cholesterol competition.^[74] The global phytosterol market, where campesterol represents a major component alongside beta-sitosterol, was valued at approximately USD 1.06 billion in 2024 and is projected to grow at a compound annual growth rate of 9.4% through 2030, as of 2024 data.^[75] This growth reflects increased fortification in everyday products and regulatory support for health claims.^[76]

Pharmaceutical and Industrial Uses

Campesterol serves as a key precursor in the synthesis of boldenone undecylenate, an anabolic steroid employed in veterinary medicine to promote growth in animals, through processes involving hydrogenation and structural modifications of phytosterols derived from plant sources.^[77] Microbial biotransformation methods, such as those using engineered strains of Yarrowia lipolytica, enable efficient conversion of campesterol and related sterols into boldenone intermediates like androsta-1,4-diene-3,17-dione.^[78] In pharmaceutical research, campesterol has been investigated for its potential in developing anti-inflammatory agents, with derivatives demonstrating reduced nociception and antioxidant effects in models of arthritis and inflammation.^[55] Its anti-inflammatory properties stem from modulation of pro-inflammatory cytokines, positioning it as a candidate for therapeutic applications in rheumatoid arthritis.^[57] Additionally, campesterol acts as an adjuvant in cancer therapies, particularly for estrogen receptor-positive breast cancer, where it inhibits ERα activity and reduces tumor growth in patient-derived organoids.^[79] Studies in mammary tumor models further indicate that campesterol supplementation decreases tumor incidence and cellular proliferation.^[80] Industrially, campesterol enhances skin barrier function in cosmetics, where it is incorporated into formulations like moisturizers and lotions to repair damaged skin and alleviate conditions such as eczema and psoriasis.^[81] Safety assessments confirm its suitability for topical use, with low absorption and minimal irritation potential.^[81] In analytical chemistry, purified campesterol functions as a reference standard for gas chromatography (GC) and high-performance liquid chromatography (HPLC) assays of sterols in plant oils and biological samples.^[82] Commercial production of campesterol relies on microbial fermentation using genetically modified yeasts to overproduce it from simple carbon sources, or chemical extraction and purification from plant oils via chromatography and subcritical fluid methods.^[83] These approaches yield high-purity campesterol for pharmaceutical and industrial applications.^[84] Regarding regulatory status, campesterol, as a component of phytosterol mixtures, is affirmed as generally recognized as safe (GRAS) by the U.S. Food and Drug Administration (FDA) for use in food products at levels up to 12% free phytosterols in spreads and similar items.^[85] However, its derivatives like boldenone are subject to strict controls under veterinary drug regulations due to their anabolic properties.

References

[1]
Phytosterols and the Digestive System: A Review Study from ... - NIH
Apr 26, 2024 · Campesterol is better absorbed than β-sitosterol, while stigmasterol is the least absorbed [14]. They are structurally similar to cholesterol ...
[2]
Campesterol - an overview | ScienceDirect Topics
Campesterol is a phytosterol with a cholesterol core, a methyl group at C24, and is essential for plant cell membrane structure.
[3]
Optimization of Campesterol-Producing Yeast Strains as a Feasible ...
Mar 27, 2023 · Campesterol is a major phytosterol that plays important roles in regulating membrane properties and serves as the precursor to multiple ...
[4]
Phytosterols: From Preclinical Evidence to Potential Clinical ...
A wide number of studies have reported remarkable pharmacological effects of PSs, acting as chemopreventive, anti-inflammatory, antioxidant, antidiabetic, and ...Abstract · Introduction · Preclinical Pharmacological... · Phytosterols in Clinical Trials
[5]
Campesterol | C28H48O | CID 173183 - PubChem - NIH
Campesterol | C28H48O | CID 173183 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities ...
[6]
Campesterol | C28H48O - ChemSpider
Molecular formula: C28H48O. Average mass: 400.691. Monoisotopic mass ... [IUPAC name – generated by ACD/Name]. (3β,24R)-Ergost-5-en-3-ol. [German].
[7]
Campesterol - the NIST WebBook
Campesterol. Formula: C28H48O; Molecular weight: 400.6801. IUPAC Standard InChI: InChI=1S/C28H48O/c1-18(2)19(3)7-8-20(4)24-11-12-25-23-10-9-21-17-22(29)13-15 ...
[8]
Campesterol - an overview | ScienceDirect Topics
Campesterol is converted to BL via several parallel pathways termed early and late C6 oxidation and early and late C22 hydroxylation.
[9]
CAMPESTEROL | 474-62-4 - ChemicalBook
Nov 2, 2025 · CAS No. 474-62-4. Chemical Name: CAMPESTEROL. Synonyms: Campesterin;Ergost-5-en-3-ol;24α-methyl Cholesterol;(24R)-Ergost-5-en-3β-ol;C01789 ...Missing: IUPAC | Show results with:IUPAC<|control11|><|separator|>
[10]
https://www.medchemexpress.com/Campesterol.html
[11]
Current methodologies for phytosterol analysis in foods
The most common and low-cost detection systems used for sterol determination include UV (200–210 nm), DAD, refractive index (RI), evaporative light scattering ...
[12]
NMR (400 MHz, CDCl 3 ) data for campesterol (1). - ResearchGate
The C=C stretching at 1634 cm⁻¹ and C-H bending at 1465 cm⁻¹ confirmed its unsaturated sterol structure. ¹H NMR spectroscopy further confirmed the structure ...
[13]
Chemical Properties of Campesterol (CAS 474-62-4) - Cheméo
log10WS : Log10 of Water solubility in mol/l. logPoct/wat : Octanol/Water partition coefficient. McVol : McGowan's characteristic volume (ml/mol). Pc ...<|separator|>
[14]
Biosynthesis and the Roles of Plant Sterols in Development ... - MDPI
Feb 20, 2022 · In this review, we summarize and discuss the recent findings in the field of plant sterols, including their biosynthesis, regulation, functions, as well as the ...
[15]
Dual biosynthetic pathways to phytosterol via cycloartenol ... - PNAS
Jan 20, 2009 · Phytosterols, such as campesterol and sitosterol, are biosynthesized via cycloartenol and catalyzed by cycloartenol synthase (CAS) in higher ...Sign Up For Pnas Alerts · Results · Discussion<|control11|><|separator|>
[16]
The Sterol Methyltransferases SMT1, SMT2, and SMT3 Influence ...
In plants, cycloartenol is the primary starting compound in sterol biosynthesis and is the substrate of SMT1, although there is some promiscuity in substrate ...
[17]
Phytosterols and phytostanols in context - ScienceDirect
... vegetable foods (mg/100 g). Food, Total Phytosterols (mg/100 g). Oils and Fats. Wheat germ oil, 919. Corn oil, 909. Rapeseed oil, 668. Sunflower oil, 411.Missing: 100g | Show results with:100g
[18]
Phytosterols: From Preclinical Evidence to Potential Clinical ...
In this sense, the present review intends to provide in-depth data on PS therapeutic potential for human health, also emphasizing their preclinical effects and ...Phytosterols In Clinical... · Phytosterols And Cancer Risk · In Vitro Phytosterol...<|control11|><|separator|>
[19]
Phytosterol Contents of Edible Oils and Their Contributions to ...
Aug 9, 2019 · The main sources of phytosterols were edible vegetable oils (46.3%), followed by cereals (38.9%), vegetables (9.2%), nuts (2.0%), fruits (1.5%), ...
[20]
Campesterol Rich Foods - Medindia
Find Foods Highest in Campesterol (per 100 gram Serving) · 1. Oil, canola (241mg) · 2. Oil, corn and canola (233mg) · 3. Oil, vegetable, Natreon canola, high ...
[21]
Phytosterol composition of nuts and seeds commonly consumed in ...
Nov 30, 2005 · Sesame seed and wheat germ had the highest total phytosterol content (400-413 mg/100 g) and Brazil nuts the lowest (95 mg/100 g).Missing: 100g | Show results with:100g
[22]
Contents of Phytosterols in Vegetables and Fruits Commonly ...
The phytosterol contents in fruits ranged 1.6-32.6 mg/100 g, the highest concentration was found in navel orange, tangerine, and mango.Missing: 100g | Show results with:100g
[23]
Plant sterol-enriched margarine lowers plasma LDL in ... - PubMed
The plant sterol content consisted of sitosterol esters (50%), campesterol esters (25%), stigmasterol esters (20%) and 10% of other esters.
[24]
Phytosterols | Linus Pauling Institute | Oregon State University
Phytosterols are found in all plant foods, but the highest concentrations are found in unrefined plant oils, including vegetable, nut, and olive oils (3). Nuts, ...
[25]
Mayo Clinic Proceedings
19-21 Vegetarians consume about 325 mg/d of sitos- terol and about 60 mg/d of campesterol.22 Thus, the usual daily intake of plant sterols and stanols ranges ...<|control11|><|separator|>
[26]
Advances and Challenges in Plant Sterol Research - PubMed Central
Plant sterols (PS) are cholesterol-like terpenoids widely spread in the kingdom Plantae. Being the target of extensive research for more than a century.
[27]
Phytosterols: Applications and recovery methods - ScienceDirect
Phytosterols are isolated from tall oil soap, also called soap skimmings, from crude tall oil and from tall oil pitch (Wong et al., 1999, Hamunen, 2003, Schultz ...
[28]
Sterols in plant biology – Advances in studying membrane dynamics
May 29, 2025 · This review synthesizes our current understanding of plant sterols at the cell surface, while highlighting emerging methodological advances.
[29]
The role of sterols in plant response to abiotic stress
Jul 10, 2020 · Maintenance of membrane homeostasis represents one of the principal functions of sterols in plant cells. Plant cell membranes are important ...
[30]
Sterols in plant biology – Advances in studying membrane dynamics
Sterols regulate membrane fluidity and protein functions at the plasma membrane, making up about one third of the PM lipids.
[31]
Plant Sterols: Structures, Biosynthesis, and Functions
The biosynthesis of plant sterols starts with the mevalonate pathway, a metabolic pathway found in most organisms. This pathway begins with the conversion of ...Structures And Occurrence Of... · Biosynthesis Of Plant... · Function Of Plant Sterols
[32]
Membrane Sterol Composition in Arabidopsis thaliana Affects Root ...
Plant membrane sterol composition has been reported to affect growth and gravitropism via polar auxin transport and auxin signaling.
[33]
Manipulating brassinosteroid signaling pathway to genetically ... - NIH
Simplified brassinosteroid biosynthetic pathways in Arabidopsis thaliana starting from campesterol ... brassinosteroid-responsive gene expression and plant growth ...
[34]
Brassinosteroids in maize: Biosynthesis, signaling pathways, and ...
BES1 accumulates in the nucleus in response to brassinosteroids to regulate gene expression and promote stem elongation. Cell, 109 (2002), pp. 181-191 ...
[35]
Arabidopsis Mutants Reveal Multiple Roles for Sterols in Plant ...
The ratio of campesterol to sitosterol has been shown to modulate Arabidopsis growth. Plants overexpressing SMT2 have increased sitosterol/campesterol ratios ...Missing: pollen | Show results with:pollen
[36]
Effects of impaired steryl ester biosynthesis on tomato growth and ...
Sep 28, 2022 · Our results indicate that SlPSAT1 has a predominant role in tomato SE biosynthesis while SlASAT1 would mainly regulate the flux of the sterol pathway.
[37]
Full article: The role of phytosterols in plant adaptation to temperature
The fine tuning of the sterol structure thus appears to be the evolution response for plant adaptation to large temperature variations.
[38]
The role of phytosterols in plant adaptation to temperature - PMC
It thus appears that a fine tuning of the sterol structure, i.e., the presence of branched ethyl groups in plant sterols increasing membrane cohesion through ...
[39]
Comparison of intestinal absorption of cholesterol with different plant ...
A positive correlation between the absorption rate of cholesterol and campesterol was established. In addition, there was a negative correlation between the ...
[40]
New Concepts of Mechanisms of Intestinal Cholesterol Absorption
These micelles function as a concentrated reservoir and transport vehicle for cholesterol across the unstirred water layer toward the brush border of the small ...
[41]
https://doi.org/10.1016/0024-3205(95)00263-6
[42]
Comparison of the hepatic clearances of campesterol, sitosterol, and ...
This study was therefore conducted to determine the biliary secretion rates and hepatic clearances of campesterol, sitosterol, and cholesterol.
[43]
Plasma Plant Sterol Levels: Another Coronary Heart Disease Risk ...
Jan 1, 2005 · In Western populations, the major plasma plant sterols are campesterol (≈0.33 mg/dL) and sitosterol (≈0.25 mg/dL), with all others ...
[44]
The role of Niemann-Pick C1 – Like 1 (NPC1L1) in intestinal sterol ...
In the lumen, phytosterols and other non-cholesterol markers must compete with large amount of biliary and dietary cholesterol for incorporation into these ...
[45]
The Bioavailability and Biological Activities of Phytosterols as ... - NIH
Jan 14, 2022 · It is well known that the gut is the main site of cholesterol absorption. Some of the cholesterol in food and bile is reabsorbed in the ...
[46]
Niemann-Pick C1 Like 1 (NPC1L1) Is the Intestinal Phytosterol and ...
Niemann-Pick C1 Like 1 (NPC1L1) is a protein localized in jejunal enterocytes that is critical for intestinal cholesterol absorption.
[47]
Plant sterols/stanols as cholesterol lowering agents: A meta-analysis ...
Consumption of plant sterols has been reported to reduce low density lipoprotein (LDL) cholesterol concentrations by 5–15%. Factors that affect plant sterol ...
[48]
Efficacy and Safety of Plant Stanols and Sterols in the Management ...
Foods with plant stanol or sterol esters lower serum cholesterol levels. We summarize the deliberations of 32 experts on the efficacy and safety of sterols ...
[49]
Effects of phytosterol supplementation on lipid profiles... - Medicine
Oct 18, 2024 · Our findings suggest that dietary phytosterols can effectively promote the reduction of TC, LDL-c, and Apo-B, along with increased high-density lipoprotein ...
[50]
Effects of phytosterol supplementation on lipid profiles in patients ...
Apr 3, 2023 · Our findings suggest that dietary phytosterols can help reduce TC and LDL-C concentrations in hypercholesterolemia patients without affecting HDL-C and TG ...
[51]
Original Article Additive effects of plant sterols supplementation in ...
Plant sterol supplementation reduces LDL-C in ∼10%. · Statins by reducing cholesterol synthesis can increase cholesterol absorption. · Plant sterols added to ...
[52]
(PDF) The Effect of Adding Plant Sterols or Stanols to Statin Therapy ...
Aug 6, 2025 · Upon meta-analysis, the use of plant sterols/stanols in combination with statin therapy significantly lowered total cholesterol (WMD, -14.01 mg/ ...
[53]
Serum plant sterols and their relation to cholesterol absorption
The levels of the phytosterols in whole serum and in each lipoprotein were significantly correlated with the percentage absorption of dietary cholesterol but ...
[54]
Serum plant sterols and biliary cholesterol secretion in humans
Ratios of cholestanol, campesterol, and sitosterol to cholesterol in serum are known to reflect cholesterol absorption efficiency.
[55]
Mechanistic evaluation of antiarthritic and anti-inflammatory effect of ...
Jan 23, 2024 · The results revealed that Campesterol Ester Derivatives exhibited significant anti-arthritis impacts, leading to paw oedema reduction and ...
[56]
(PDF) Mechanistic Evaluation of Anti-Arthritic Effects of Campesterol ...
Jun 26, 2023 · The qPCR analysis showed that treatment with campesterol significantly reduced pro-inflammatory cytokines TNF-a, NF- κB, IL-6, COX II, and IL-1 ...
[57]
Campesterol: A Natural Phytochemical with Anti Inflammatory ...
May 31, 2023 · This review presents notable findings suggesting that Campesterol appears to be particularly effective in the expression of pro-inflammatory ...
[58]
Phytosterols: Physiological Functions and Potential Application - PMC
Jun 3, 2024 · The antioxidant activity of phytosterols has been confirmed and reported in numerous studies. As a natural free-radical scavenger, the peroxyl ...
[59]
The Protective Effect of Dietary Phytosterols on Cancer Risk
Jun 23, 2019 · Many studies have explored the association between dietary phytosterols and cancer risk, but the results have been inconsistent.
[60]
Mechanisms of Phytochemicals in Anti-Inflammatory and Anti-Cancer
Campesterol increases the expression of cytochrome c, Bak, Bax, and autophagy-related proteins such as BECN1 in ovarian cancer cell lines [14]. Brassicasterol ...<|separator|>
[61]
Fatty Acid and Phytosterol Content of Commercial Saw Palmetto ...
Some research suggests that saw palmetto phytosterols (β-sitosterol, campesterol, stigmasterol), inhibit 5α-reductase, prostate cancer cell/tumor growth, and/or ...
[62]
Phytosterols as modulators of gut-brain axis and neuroinflammation ...
Gut dysbiosis and neuroinflammation worsen Alzheimer's pathology. · Phytosterols modulate gut microbiota and SCFAs, lowering neuroinflammation in Alzheimer's ...Review · Phytosterol And Their... · Neuroprotective Property
[63]
Plasma fat-soluble vitamin and carotenoid concentrations after plant ...
Plant sterols and stanols interfere with intestinal cholesterol absorption, and it has been questioned whether absorption and plasma concentrations of fat- ...
[64]
Sitosterolemia (Phytosterolemia) - StatPearls - NCBI Bookshelf - NIH
For affected individuals, serum levels are often in the range of 10 to 65 mg/dL depending on the severity of the disease. Tests can be falsely negative if a ...
[65]
Genome-wide meta-analysis of phytosterols reveals five novel loci ...
Jan 10, 2022 · We perform a genome-wide meta-analysis of 32 phytosterol traits reflecting resorption, cholesterol synthesis and esterification in six studies with up to 9758 ...
[66]
Safety of the extension of use of plant sterol esters as a novel food ...
Jun 30, 2020 · When considering an intake of the maximum authorised use level of 3 g plant sterols/person per day and oxidation rates of 0.5% and 2.28 ...Missing: upper limit
[67]
https://eur-lex.europa.eu/legal-content/EN/TXT/HTML/?uri=CELEX:32013R0739
[68]
Food Labeling; Health Claim; Phytosterols and Risk of Coronary ...
Dec 8, 2010 · As a result, FDA believes it would be reasonable to base the minimum qualifying amount of phytosterol in a food on four servings per day. As ...
[69]
Letter Enforcement Interim Health Claim Rule Plant Sterol/Stanol Ester
Mar 28, 2024 · The interim final rule authorizes use of a health claim on food labeling about plant sterol/stanol esters and reduced risk of coronary heart ...
[70]
Blood cholesterol reduction health claims on phytosterols can now ...
Jul 31, 2009 · EFSA experts have concluded that foods such as yoghurt and margarine containing certain levels of plant stanols and sterols can reduce blood cholesterol levels.
[71]
Phytosterols in the Treatment of Hypercholesterolemia and ... - NIH
Although phytosterols decrease LDL-cholesterol levels, there is no evidence that they reduce the risk of cardiovascular diseases.
[72]
Advancements in the Esterification of Phytosterols Catalyzed ... - MDPI
Additionally, previous studies have shown that the solubility of phytosterol unsaturated fatty acid esters in edible oils (such as soybean oil) is ten times ...
[73]
Phytosterols in low- and nonfat beverages as part of a controlled diet ...
Abstract Dietary phytosterols have been shown to reduce plasma cholesterol concentrations when consumed in dif- ferent food matrices, but their ...
[74]
Phytosterols Market Size And Share | Industry Report, 2030
The global phytosterols market size was estimated at USD 1.06 billion in 2024 and is anticipated to grow at a CAGR of 9.4% from 2025 to 2030.
[75]
Phytosterols Market Size, Share | Forecast- 2034 - Nova One Advisor
The global phytosterols market is expected to grow from USD 1.92 billion in 2025 to USD 4.38 billion by 2034, at a CAGR of 9.6%. This growth is driven by ...
[76]
Single step biotransformation of corn oil phytosterols to boldenone ...
May 26, 2016 · The authentic steroids used; β-sitosterol 99%, campesterol 98%, stigmasterol 99%, androst-4-ene 3,17-dione 98%, testosterone 99% and boldenone ...
[77]
Engineering Yarrowia lipolytica for Campesterol Overproduction
Campesterol synthesis pathway was constructed in Y. lipolytica via blocking ergosterol biosynthesis and introducing heterogeneous DHCR7. The endogenous ...
[78]
A Virtual Drug Discovery Screening Illuminates Campesterol as a ...
Jun 5, 2024 · Campesterol treatment demonstrated a time-dependent reduction in the growth of ERα-positive breast cancer PDOs. Additionally, dose-dependent ...Missing: therapies | Show results with:therapies
[79]
[PDF] Campesterol Reduces Tumor Development and Mammary Cellular ...
Jan 30, 2023 · Conclusion: The results of the present study showed that campesterol could reduce tumor size, tumor incidence, number of tumors developed and ...
[80]
[PDF] Safety Assessment of Phytosterols as Used in Cosmetics
4 The maximum reported concentration of use was 15%. Many of the phytosterols in this study are from edible plant sources. Exposure to these phytosterols from.
[81]
https://www.cir-safety.org/sites/default/files/phytosterols.pdf
[82]
Increased campesterol synthesis by improving lipid content in ...
Jun 26, 2020 · In this study, we have successfully constructed a campesterol biosynthetic pathway by modifying the synthetic pathway of ergosterol in Y. lipolytica with ...
[83]
Unveiling the approach of campesterol: Its application and circular ...
Jul 2, 2025 · Campesterol is a naturally occurring phytosterol found in the plant cell membranes, and it shows medicinal uses in nutraceuticals and ...