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Chlorophyceae

The Chlorophyceae constitute a major class of green algae within the division Chlorophyta, encompassing a highly diverse array of primarily unicellular, colonial, and filamentous organisms that predominantly inhabit freshwater and terrestrial environments.^[1] These algae are characterized by their green pigmentation from chlorophylls a and b, along with accessory pigments such as β-carotene and various xanthophylls, and they store photosynthetic reserves as starch in the chloroplast stroma, often associated with pyrenoids.^[2] Cell division in Chlorophyceae typically involves a phycoplast structure for cytokinesis, and motile cells feature two equal flagella with a distinctive clockwise basal body orientation.^[2]^[3] Phylogenetically, the Chlorophyceae form a monophyletic group within the core Chlorophyta clade, which also includes the classes Ulvophyceae, Trebouxiophyceae, Pedinophyceae, and Chlorodendrophyceae; this clade is supported by molecular data from chloroplast genes and represents an early radiation of green algal lineages.^[1] The class exhibits significant morphological variation, ranging from free-living flagellates like Chlamydomonas to complex colonial forms such as Volvox, and non-motile coccoid or filamentous types including Chlorella and Oedogonium.^[3] While most species are photosynthetic autotrophs contributing to primary production in aquatic ecosystems, some form symbiotic associations or tolerate extreme conditions, such as acidic or high-salinity habitats.^[2] Reproduction in Chlorophyceae is versatile, encompassing both asexual methods like zoospore formation and binary fission, and sexual processes including isogamy, anisogamy, and oogamy, often culminating in zygotic meiosis.^[2] The class is currently classified into five principal orders—Chaetophorales, Chaetopeltidales, Sphaeropleales, Oedogoniales, and Volvocales—based on a combination of ultrastructural features and phylogenetic analyses, though ordinal relationships continue to be refined through genomic studies.^[1] With thousands of described species, Chlorophyceae play crucial ecological roles as oxygen producers and food sources in freshwater communities, and several genera hold biotechnological promise for biofuel production and nutrient cycling.^[1]

Characteristics

Morphology

Chlorophyceae exhibit a wide array of morphological forms, ranging from simple unicellular structures to more complex multicellular organizations, reflecting their evolutionary adaptability within the green algae.^[4] This diversity includes unicellular motile or non-motile cells, such as those in Chlamydomonas, which serve as foundational models for algal structure.^[5] Colonial forms, like Volvox, organize into spherical or ellipsoidal clusters of cells, while filamentous types, exemplified by Oedogonium, form unbranched chains of cylindrical cells.^[4] Coenocytic structures, seen in genera such as Characiosiphon, feature multinucleate filaments without complete septa between cells, contributing to their structural complexity.^[5] Cell sizes in Chlorophyceae vary significantly across forms, with unicellular species like Chlamydomonas reinhardtii typically measuring about 10 μm in diameter.^[6] Filamentous forms, such as Oedogonium, have cells with diameters commonly ranging from 14 to 30 μm, extending into longer filaments.^[7] Colonial aggregates, particularly in Volvox species, can reach diameters exceeding 500 μm, up to several millimeters, forming visible globular bodies.^[8] Attached or benthic Chlorophyceae often feature specialized holdfast structures, such as the basal cell in Oedogonium filaments, which anchors the thallus to substrates like rocks or vegetation.^[7] Gelatinous matrices are prominent in colonial forms, where individual cells of Volvox are embedded within an extracellular mucilaginous sheath that provides structural integrity and protection.^[9] The basic thallus organization in Chlorophyceae progresses from unicellular prototypes, like Chlamydomonas, through colonial associations without tissue differentiation, as in Volvox, to multicellular filaments in Oedogonium, where cells align in linear series with shared walls.^[4] This gradation underscores the class's range from solitary cells to integrated multicellular assemblies, without advanced tissue specialization.^[5]

Cellular Ultrastructure

The cell walls of Chlorophyceae are often composed of cellulose microfibrils in certain taxa, such as filamentous forms like Oedogonium, which provide structural support similar to those in higher plants.^[4] In many species, particularly within the Chlamydomonas-Volvox assemblage, the walls incorporate hydroxyproline-rich glycoproteins (HRGPs) that form crystalline structures, often in the absence of significant cellulose, contributing to rigidity and protection.^[4] Additionally, some taxa feature sporopollenin or algaenan as hydrophobic components in the extracellular matrix, enhancing resistance to environmental stresses in diverse Chlorophyta lineages including Chlorophyceae.^[10] Motile cells in Chlorophyceae typically possess two or four flagella, which emerge apically from the cell anterior, enabling locomotion through a breaststroke-like motion.^[11] The flagellar apparatus is characterized by basal body orientations that are distinctive to this class, including clockwise (CW) or direct opposite (DO) arrangements, which have been key phylogenetic markers for distinguishing orders within Chlorophyceae.^[12] These orientations influence flagellar beating patterns and are evolutionarily derived from an ancestral counterclockwise (CCW) configuration in broader Chlorophyta, with CW and DO emerging in specific clades like Chlamydomonadales and Sphaeropleales.^[13]^[14] Eyespots, or stigmata, are present in the motile forms of many Chlorophyceae species, consisting of carotenoid-rich lipid globules arranged in hexagonal arrays within the chloroplast envelope.^[15] These organelles function in phototaxis by shading adjacent photoreceptors, such as channelrhodopsins in species like Chlamydomonas reinhardtii, allowing cells to detect light direction and intensity for oriented swimming toward optimal light conditions.^[15] Contractile vacuoles are prominent in freshwater Chlorophyceae species, serving as osmoregulatory organelles that expel excess water to counteract hypotonic environments.^[16] In Chlamydomonas reinhardtii, for instance, these vacuoles form a complex that collects and ejects fluid through periodic contractions, maintaining cellular turgor and ion balance essential for survival in dilute media.^[16]

Chloroplasts and Pigments

Chlorophyceae possess chloroplasts that contain the primary photosynthetic pigments chlorophyll a and chlorophyll b, which enable the absorption of light in the blue and red wavelengths for efficient photosynthesis.^[17] These chloroplasts also incorporate accessory pigments, including β-carotene, lutein, violaxanthin, neoxanthin, and other xanthophylls, which broaden the spectrum of light harvested and provide photoprotection against excess energy. This pigment composition imparts the characteristic green coloration to Chlorophyceae and distinguishes them from other algal groups lacking chlorophyll b.^[17] The morphology of chloroplasts in Chlorophyceae is highly variable, reflecting the diversity within the class, with shapes ranging from parietal (cup-shaped) to axial, girdle-like, or even stellate configurations.^[18] For instance, in unicellular species like Chlamydomonas, a single cup-shaped chloroplast occupies much of the cell volume, while colonial forms such as Volvox may contain multiple chloroplasts per cell, often numbering in the dozens.^[19] These organelles are bounded by a double membrane and feature internal thylakoids arranged in unstacked or loosely stacked grana, facilitating the light-dependent reactions of photosynthesis.^[17] A prominent feature of many Chlorophyceae chloroplasts is the presence of pyrenoids, dense, protein-rich bodies typically embedded within the stroma and surrounded by a sheath of starch granules.^[20] Pyrenoids, composed primarily of the enzyme Rubisco, function as CO₂-concentrating mechanisms to enhance carbon fixation efficiency, and they are often traversed by penetrating thylakoids that maintain continuity with the photosynthetic membrane system.^[21] This structure is particularly evident in orders like Chlamydomonadales, where pyrenoids support rapid starch accumulation under favorable conditions.^[17] Unlike many other algal lineages, Chlorophyceae store photosynthetic products as starch granules directly within the chloroplasts, either around pyrenoids or dispersed in the stroma, serving as the primary carbohydrate reserve.^[17] This intrachloroplastic localization of starch synthesis and storage underscores a key biochemical adaptation shared with land plants, optimizing energy mobilization for growth and reproduction.^[20]

Habitat and Ecology

Distribution and Environments

Chlorophyceae, a class of green algae within the phylum Chlorophyta, are predominantly distributed in freshwater habitats worldwide, including ponds, rivers, and wetlands, where they form a significant component of planktonic and benthic communities.^[12] These algae exhibit a cosmopolitan global distribution, with the highest species diversity reported in temperate and tropical freshwater systems, reflecting their adaptation to a range of climatic conditions that support prolific growth in nutrient-rich waters.^[22] While primarily limnic, some taxa occur in brackish and coastal environments.^[12] Terrestrial forms are also common, colonizing exposed surfaces such as soil and tree bark, where they contribute to microbial biofilms in humid microhabitats.^[23] Certain Chlorophyceae demonstrate remarkable tolerance to extreme environmental conditions, enabling their presence in otherwise inhospitable niches. For instance, species like Dunaliella inhabit hypersaline environments, such as salt lakes, where they endure salinities exceeding 2.5 M NaCl through osmotic adjustments and glycerol accumulation.^[24] Similarly, taxa including Chlamydomonas species show acid tolerance, persisting in acidic soils and waters with pH values as low as 2–3, often associated with mine drainage or peat bogs, via enhanced metal efflux and proton pumps.^[25] These extremophiles highlight the class's ecological versatility beyond typical aquatic realms. Symbiotic associations further expand the distributional range of Chlorophyceae, integrating them into diverse ecosystems. Some form mutualistic partnerships with fungi in lichens, providing photosynthetic capabilities in terrestrial and epiphytic settings, as seen in genera like Bracteacoccus.^[26] Others engage in endosymbioses with protozoa, such as ciliates in freshwater habitats, where coccoid Chlorophyceae supply nutrients in exchange for protection and mobility.^[27] Terrestrial Chlorophyceae often possess adaptations like mucilaginous sheaths that enhance desiccation resistance by retaining moisture and shielding cells from UV radiation and drying winds on exposed substrates.^[28] These extracellular polysaccharides allow survival during intermittent wetting-drying cycles, facilitating colonization of bark and soil in variable humidity environments.^[29]

Ecological Roles

Chlorophyceae, as primary producers in freshwater and coastal aquatic ecosystems, play a crucial role in oxygen production through photosynthesis, contributing significantly to atmospheric oxygen levels and supporting aerobic life forms.^[30] They also facilitate carbon cycling by fixing atmospheric CO₂ into organic matter, which serves as a carbon sink and influences global carbon budgets in aquatic environments.^[31] This photosynthetic activity underpins the productivity of wetlands, lakes, and rivers, where Chlorophyceae dominate phytoplankton communities. In food webs, Chlorophyceae form the foundational layer, serving as a primary food source for zooplankton, herbivorous fish, and invertebrates, thereby transferring energy upward through trophic levels.^[32] Certain species act as bioindicators of water quality; for instance, blooms of genera like Scenedesmus signal eutrophication due to elevated nutrient levels from agricultural runoff or sewage, allowing assessment of pollution impacts.^[33] Additionally, they contribute to nutrient cycling by assimilating nitrogen and phosphorus, reducing excess nutrients in water bodies and preventing further eutrophication.^[34] Economically, Chlorophyceae species hold substantial value; Chlorella vulgaris is cultivated for nutritional supplements rich in proteins, vitamins, and antioxidants, supporting human health applications like immune enhancement.^[35] Dunaliella salina is a key commercial source of β-carotene, used in food coloring and as a provitamin A supplement, with production enhanced under high-salinity conditions.^[36] Furthermore, species like Chlorella show promise in biofuels, yielding lipids for biodiesel, and in phycoremediation, where they remove heavy metals and nutrients from wastewater, offering sustainable environmental cleanup.^[37] However, excessive growth of Chlorophyceae can lead to harmful blooms in nutrient-enriched waters, forming dense algal mats that reduce light penetration, deplete oxygen upon decomposition, and disrupt aquatic habitats, exacerbating issues like fish kills in eutrophic lakes.

Reproduction

Asexual Reproduction

Asexual reproduction in Chlorophyceae is a primary mechanism for rapid propagation and population expansion, occurring through various spore types and vegetative means without genetic recombination. This process allows these green algae to colonize new environments efficiently, particularly in freshwater habitats where conditions fluctuate. Common methods include the production of motile and non-motile spores, as well as fragmentation, enabling survival and dispersal under diverse ecological pressures.^[12]^[38] Zoospore formation is one of the most prevalent asexual reproductive strategies in Chlorophyceae, especially in unicellular and colonial forms such as those in the orders Volvocales and Sphaeropleales. Zoospores are biflagellate, pear-shaped, motile spores produced within sporangia through repeated mitotic divisions of the parent cell. Upon maturation, the sporangium wall ruptures, releasing the zoospores, which swim briefly using their flagella before settling, shedding flagella, and developing into new vegetative cells. This method facilitates quick dispersal in aquatic settings, as seen in genera like Chlamydomonas and Volvox.^[38]^[39]^[40] Non-motile spores, including aplanospores and autospores, serve as dormant or dispersive structures, aiding survival during adverse conditions like desiccation or nutrient scarcity. Aplanospores are thick-walled, non-flagellated cells formed inside the parent sporangium, which are released upon wall rupture and germinate when conditions improve. Autospores, similarly non-motile and produced by mitosis, are characteristic of coccoid forms in Sphaeropleales, such as Scenedesmus, where they emerge directly from the parent cell without an intervening sporangial phase. These spores emphasize vegetative persistence over active motility.^[12]^[39]^[41] In filamentous Chlorophyceae, such as Oedogonium and genera in Chaetophorales like Chaetophora, asexual reproduction often occurs via fragmentation, where the filament breaks into segments due to mechanical stress or cell death at weakened points, with each fragment regenerating into a complete thallus. Additionally, akinetes form as specialized, thick-walled, dormant cells in certain genera like Haematococcus, accumulating reserves to withstand harsh environmental stresses such as extreme temperatures or drying. These akinetes germinate under favorable conditions to restore vegetative growth. The palmella stage represents a temporary encystment response to stress, where motile cells, as in Chlamydomonas, lose flagella and embed in a gelatinous matrix, forming non-motile aggregates that resume motility upon environmental recovery. This stage enhances resilience in fluctuating habitats.^[40]^[42]^[43]^[44]

Sexual Reproduction

Sexual reproduction in Chlorophyceae is characterized by a haplontic life cycle, in which the multicellular or unicellular haploid phase dominates, and meiosis occurs zygotically in the diploid zygote following gamete fusion.^[45] Gametes are typically produced mitotically within specialized gametangia, such as oogonia and antheridia, or directly from vegetative cells under environmental cues like nutrient limitation.^[46] This process promotes genetic diversity through recombination during meiosis in the zygote, contrasting with the clonal propagation seen in asexual reproduction. The class exhibits a range of gamete fusion types, from primitive isogamy to advanced oogamy, reflecting evolutionary adaptations for sexual reproduction. Isogamy involves the fusion of morphologically similar, biflagellated gametes of equal size, as observed in unicellular species like Chlamydomonas reinhardtii, where plus (+) and minus (-) mating types pair to form a zygote.^[47] Anisogamy features gametes of unequal size but both motile, representing an intermediate stage toward differentiation. Oogamy, the most derived form, entails the union of a large, non-motile egg within an oogonium and small, flagellated sperm from an antheridium, exemplified by filamentous Oedogonium species where dwarf males facilitate fertilization.^[48] Upon fusion, the zygote develops into a thick-walled zygospore, a resting structure that enhances survival against desiccation and adverse conditions by accumulating storage reserves and sporopollenin-like polymers in its multilayered wall.^[49] These zygospores germinate under favorable conditions, releasing haploid meiospores to restart the life cycle.

Taxonomy and Phylogeny

Classification History

The classification of Chlorophyceae, a major class within the green algae (Chlorophyta), originated in the early 19th century with systems emphasizing pigmentation and reproductive structures. Carl Adolph Agardh, in his 1824 Systema Algarum, grouped green algae under the subclass Confervoideae based on their characteristic chlorophyll pigmentation and simple filamentous or unicellular morphologies, distinguishing them from red and brown algae primarily by color and basic thallus form. This approach, shared by contemporaries like C.A. Agardh's son Jacob Georg, focused on observable traits such as reproduction via zoospores or gametes, laying the foundation for recognizing green algae as a cohesive group without ultrastructural details. By the mid-20th century, F.E. Fritsch's influential 1935 classification in The Structure and Reproduction of the Algae refined these ideas, elevating Chlorophyceae to class status within Chlorophyta and dividing it into orders based on pigmentation (chlorophylls a and b), reserve products (starch), flagellar insertion (lateral or apical), and reproductive modes (isogamous to oogamous). Fritsch's system grouped motile and non-motile forms together, using cell wall chemistry—such as the presence of cellulose or hemicellulose—as a supplementary criterion, though it retained broad groupings that later proved artificial. In the 1970s and 1980s, advancements in electron microscopy introduced flagellar ultrastructure as a key divisor, with Picket-Heaps and Marchant (1972) highlighting differences in basal body orientation and rootlet systems between motile (e.g., Chlamydomonas-like) and non-motile forms. A pivotal shift occurred in the 1980s and 1990s with detailed analyses of flagellar apparatus configurations, particularly the orientation of basal bodies and microtubule rootlets. Mattox and Stewart (1984) proposed separating Chlorophyceae (featuring directly opposed [DO] or clockwise [CW] basal body arrangements) from Ulvophyceae (counterclockwise [CCW] orientation), using cruciate root systems (X-2-X-2 patterns) to delineate evolutionary lines based on cell division and cytokinesis. O'Kelly and Floyd (1984) further corroborated this by correlating these ultrastructural traits with reproductive and mitotic patterns, effectively excluding CCW-configured taxa from traditional Chlorophyceae and emphasizing cell wall chemistry (e.g., mannan vs. cellulose) as confirmatory evidence. The advent of molecular phylogenetics in the late 1990s and 2000s exposed the polyphyly of the traditional Chlorophyceae, as 18S rRNA and multi-gene analyses showed that many included taxa nested within separate lineages. Friedl (1995) demonstrated polyphyly in coccoid genera like Chlorococcum using rDNA sequences, prompting the erection of new classes such as Trebouxiophyceae for non-flagellate, CCW-related forms. By the early 2000s, comprehensive reviews integrated these data, resolving Chlorophyceae as a monophyletic core clade with DO/CW configurations, while broader green algal diversity was restructured to reflect ancient divergences from prasinophytes and charophytes.

Current Classification

Chlorophyceae represents the core class of green algae within the phylum Chlorophyta, encompassing a diverse array of primarily freshwater unicellular, colonial, and filamentous forms that store starch as their primary reserve polysaccharide. This class is estimated to include approximately 4,000–7,000 species, based on ongoing taxonomic assessments in databases like AlgaeBase as of 2023.^[50] The modern taxonomic framework for Chlorophyceae is structured around two primary monophyletic clades derived from molecular phylogenetic analyses: the SV clade (Sphaeropleales and Volvocales) and the OCC clade (Oedogoniales, Chaetophorales, and Chaetopeltidales). Additional orders, such as Microsporales (often subsumed under Sphaeropleales), and incertae sedis groups contribute to the class's complexity, reflecting unresolved positions in some lineages. Representative families include Chlamydomonadaceae (within Volvocales or synonymized Chlamydomonadales), Scenedesmaceae (within Sphaeropleales), and Oedogoniaceae (within Oedogoniales), which collectively highlight the morphological diversity from flagellated monads to branched filaments. Recent revisions, particularly in AlgaeBase updates through 2023, have refined this hierarchy by elevating certain basal lineages previously included in Chlorophyceae—such as those now classified as the separate class Pedinophyceae—based on phylogenomic evidence distinguishing their flagellar apparatus and chloroplast structure from core chlorophycean taxa. These changes underscore the dynamic nature of chlorophycean taxonomy, integrating ultrastructural, molecular, and ecological data to delineate boundaries within Chlorophyta.^[50]

Phylogenetic Relationships

Molecular phylogenetic analyses have consistently supported the monophyly of core Chlorophyceae, a class within the Chlorophyta, with high bootstrap support in multi-gene studies utilizing markers such as 18S rDNA and rbcL, often exceeding 90% in chloroplast genome-based phylogenies.^[51] These findings are reinforced by larger datasets incorporating up to 53 chloroplast genes, which resolve the class as a well-defined clade distinct from other green algal groups.^[51] Within Chlorophyceae, two primary lineages emerge prominently: the Sphaeroplea-Volvox (SV) clade, encompassing orders like Sphaeropleales and Volvocales, and Chlorophyceae sensu stricto, which includes groups such as Chaetophorales.^[52] This bipartition is upheld across analyses of concatenated chloroplast protein-coding genes (e.g., 58 genes from 68 taxa), though ordinal-level relationships within these lineages exhibit some uncertainty, with bootstrap supports varying but generally robust for the major split.^[52] In the broader context of Chlorophyta, Chlorophyceae forms part of the monophyletic core Chlorophyta alongside other classes such as Trebouxiophyceae and Ulvophyceae, with Streptophyta (including land plants) serving as the primary outgroup in phylogenetic reconstructions.^[51] Multi-gene chloroplast datasets confirm this topology, showing core Chlorophyta with posterior probabilities >0.95 and bootstrap values >90%, highlighting the evolutionary divergence of these green algal lineages from streptophyte ancestors.^[51]

Diversity and Evolution

Major Groups and Examples

The class Chlorophyceae exhibits remarkable morphological and ecological diversity, encompassing unicellular, colonial, and filamentous forms primarily adapted to freshwater habitats. It includes approximately 350 genera and 2,650 species, with the highest diversity concentrated in freshwater ecosystems and notable endemism in isolated lakes such as ancient crater lakes where unique evolutionary radiations have occurred.^[53]^[54] Unicellular members of Chlorophyceae, such as Chlamydomonas reinhardtii, represent foundational models for studying cellular processes like phototaxis, where flagellar beating enables directed swimming toward light sources.^[55] Another prominent example is Haematococcus pluvialis, renowned for its ability to accumulate high levels of the antioxidant astaxanthin under stress conditions, reaching up to 4% of dry weight.^[56] These motile, single-celled algae often inhabit temporary pools or planktonic communities in freshwater bodies. Colonial forms illustrate evolutionary transitions toward multicellularity within Chlorophyceae, particularly in the Volvocales order. Volvox species form spherical colonies of thousands of biflagellate cells embedded in a gelatinous matrix, exhibiting oogamous sexual reproduction where large non-motile eggs are fertilized by small, flagellated sperm.^[9] Similarly, Eudorina produces ovoid or ellipsoidal colonies of 16 to 32 cells arranged peripherally in a hollow sphere, showcasing coordinated motility and division patterns typical of volvocine algae.^[57] These structures enhance survival in dynamic freshwater environments like ponds and ditches. Filamentous representatives highlight the class's structural complexity, often forming unbranched chains in benthic or planktonic freshwater settings. Oedogonium species develop attached, unbranched filaments with distinctive cap-like structures (holdfasts and growth rings) on cylindrical cells, facilitating adhesion to submerged substrates.^[58] These forms dominate in nutrient-rich, slow-moving waters, contributing to the visible green mats in many aquatic systems.

Evolutionary Significance

The Chlorophyceae, a major class within the Chlorophyta, trace their evolutionary origins to the ancient Archaeplastida lineage, which arose approximately 1.5 billion years ago through the primary endosymbiosis of a cyanobacterium by a eukaryotic host.^[59] This event established the foundation for photosynthetic eukaryotes, with Chlorophyceae emerging as a derived clade within the green plants (Viridiplantae) from prasinophyte-like ancestors, a group of early-diverging, scaly flagellates that represent the ancestral stock of all green algae.^[60] These prasinophyte precursors, characterized by simple unicellular forms and diverse scale morphologies, provided the basal framework from which core chlorophytes, including Chlorophyceae, diversified during the Proterozoic Eon.^[61] A hallmark of Chlorophyceae evolution is the development of key cellular innovations that enhanced adaptability in aquatic environments. The phycoplast, a distinctive microtubule array formed during cytokinesis, replaced earlier division mechanisms and facilitated efficient cell separation in multicellular forms, marking a significant departure from the phragmoplast-mediated division seen in the sister Streptophyta lineage.^[62] Complementing this, the evolution of the pyrenoid—a proteinaceous structure within the chloroplast—enabled enhanced CO₂ concentration around Rubisco, improving photosynthetic efficiency under low-carbon conditions through the operation of carbon-concentrating mechanisms (CCMs).^[63] These adaptations likely contributed to the ecological success of Chlorophyceae, allowing proliferation in diverse freshwater and marine habitats while the Streptophyta lineage gave rise to transitional forms leading to land plants (embryophytes).^[64] The fossil record underscores the deep antiquity of Chlorophyceae-like green algae, with Proterozoic biomarkers such as C₂₈ and C₂₉ steranes indicating an early radiation of chlorophyte algae by at least 800 million years ago, potentially extending back to the Mesoproterozoic.^[65] These chemical signatures, preserved in ancient sediments, suggest that green algal ancestors played a pivotal role in shaping early oxygenated oceans. Modern extremophile Chlorophyceae, such as acidophilic species thriving in low-pH environments, serve as analogs for inferring the resilience of these early lineages under primordial Earth conditions, including fluctuating atmospheric CO₂ and nutrient scarcity.^[25]

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Jan 6, 2023 · Microalgae and cyanobacteria are responsible for the sequestration of most of the CO2 by primary producers for various biosynthetic processes ...Missing: Chlorophyceae | Show results with:Chlorophyceae
[32]
Green Algae (Chlorophyta): Characteristic, Class, Importance
Jun 19, 2025 · The class Chlorophyceae is the biggest and most varied within the green algae, found in both freshwater and marine aquatic ecosystems. They are ...
[33]
Algae as an Indicator of Water Quality - IntechOpen
Jun 29, 2016 · Algae are used as water quality indicators due to their rapid reproduction, nutrient needs, and quick response to water chemistry changes, ...Missing: Chlorophyceae | Show results with:Chlorophyceae
[34]
Biogeochemical Role of Algae in Aquatic Ecosystems - MDPI
Dec 1, 2022 · Algae play a key role in the biogeochemical fate of many chemical elements and in the regulation of their cycling in the aquatic ecosystems.
[35]
Chlorella vulgaris as a food substitute: Applications and benefits in ...
Nov 18, 2024 · Rich in protein, lipids, carbohydrates, vitamins, and minerals, Chlorella offers substantial nutritional benefits, positioning it as a valuable food substitute.Missing: Chlorophyceae | Show results with:Chlorophyceae
[36]
Enhanced β-carotene and Biomass Production by Induced ... - NIH
The chlorophyte Dunaliella salina is the supreme and natural source of β-carotene, a carotenoid with bioactive roles such as provitamin A, colorant, antioxidant ...
[37]
(PDF) Phycoremediation and the potential of sustainable algal ...
This study was undertaken to evaluate the remediation of wastewater by the green alga Chlorella vulgaris and the potential of these alga (biomass) to produce ...
[38]
[PDF] PHYCOLOGY ALGAE: GENERAL CHARACTERISTICS
Asexual reproduction may be of the following types-. (a) By zoospores-It is the commonest method of asexual reproduction algae. Zoospores are the naked ...
[39]
[PDF] Chlorophyta
... Chlorophyceae. (T1), a sister relationship between the Bryopsidales and ... •Asexual reproduction by forming zoospores, autospores, and aplanospores.
[40]
https://www.uou.ac.in/sites/default/files/slm/MSCBOT-502.pdf
[41]
[PDF] PLANT GROUPS
Asexual reproduction is achieved by Zoospores,. Aplanospores, Autospores ... Chlorophyceae. Order. Chlorococcales. Family. Chlorellaceae. Genus. Chlorella.
[42]
oedogonia (chlorophyceae) akinetes - Wiley Online Library
Our results and a review of the literature indicate that akinetes of Pithophora, other chlorophyte algae such as Zygnema, and cyanophytes do share several ...
[43]
Palmella - an overview | ScienceDirect Topics
Palmella is a genus of amorphous gelatinous colonies, a non-motile reproductive phase where cells lose flagella and form a gelatinous aggregation.
[44]
Salinity-Induced Palmella Formation Mechanism in Halotolerant ...
May 23, 2017 · When entering palmella stage, the cells usually lose their flagella and eyespot, become round, and excrete a slime exopolysaccharides (EPSs) ...
[45]
Phycokey - Coleochaete - UNH Center for Freshwater Biology
Typical of all photosynthetic protists except diatoms, the zygote (2N) undergoes meiosis prior to mitosis, thus the life cycle is called haplontic (1N nuclei).
[46]
A comparative test of the gamete dynamics theory for the evolution ...
Mar 3, 2021 · While some taxa have a diplontic life cycle in which a diploid adult (=fully grown) stage arises directly from the zygote, many taxa have a ...
[47]
Gamete dimorphism of the isogamous green alga (Chlamydomonas ...
Dec 6, 2022 · The gametes of chlorophytes differ morphologically even in isogamy and are divided into two types (α and β) based on the mating type- or ...
[48]
Morphology and Molecular Phylogeny of Genus Oedogonium ... - NIH
Sep 16, 2022 · Sexual reproduction is by oogonia and spermatozoids; oogonia are single or in groups, arising as a result of division of a vegetative cell ...Missing: oogamy | Show results with:oogamy
[49]
Induction of Conjugation and Zygospore Cell Wall Characteristics in ...
Aug 23, 2021 · Zygnematophyceae thriving in these regions possess a special means of sexual reproduction, termed conjugation, leading to the formation of ...
[50]
Studies on conjugation of Spirogyra using monoclonal culture - PMC
After elongation of the protrusion, two cells are connected by a conjugation tube and a zygote is finally formed. Two types of conjugation have been reported in ...
[51]
AlgaeBase :: Listing the World's Algae
Listing the World's Algae. AlgaeBase is a global algal database of taxonomic, nomenclatural and distributional information.Content · Genus Search · Species Search · AboutMissing: Chlorophyceae | Show results with:Chlorophyceae
[52]
New phylogenetic hypotheses for the core Chlorophyta based on ...
Oct 16, 2014 · Our study seeks to test these hypotheses by combining high throughput sequencing data from the chloroplast genome with increased taxon sampling.
[53]
Order, please! Uncertainty in the ordinal-level classification ... - PeerJ
May 15, 2019 · Background Chlorophyceae is one of three most species-rich green algal classes and also the only class in core Chlorophyta ... et al., 2015; ...
[54]
Introduction to the Chlorophyceae
Chlorophyceae are a large group of freshwater green algae with diverse shapes, including unicellular, colonies, and filaments, and a haploid life cycle.Missing: morphology | Show results with:morphology
[55]
[PDF] investigating biodiversity in endangered crater lakes of the Amber ...
This high level of endemism predominantly results from radiation of African founder individuals arriving during the Cenozoic (65.5 Ma to present) and from.
[56]
Phototaxis Assay for Chlamydomonas reinhardtii - PMC - NIH
Jun 20, 2017 · The unicellular green alga Chlamydomonas reinhardtii is used as a model organism in various research fields including phototaxis of ...
[57]
Astaxanthin-Producing Green Microalga Haematococcus pluvialis
Haematococcus pluvialis is the richest source of natural astaxanthin which is considered as “super anti-oxidant.”
[58]
Eudorina Ehrenberg, 1832 - AlgaeBase
Colonies ovoid, ellipsoidal or cylindrical, containing 16 or 32 cells arranged radially in the periphery of a gelatinous matrix, forming a hollow sphere.
[59]
Oedogonium Link ex Hirn, 1900 - AlgaeBase
Description: Unbranched uniseriate filaments attached to substratum by basal holdfast cells; occasionally free-floating. Vegetative cells generally uniform in ...
[60]
Zygnematales
The process begins with a parallel pairing of two filaments, aligning neighboring cells. Then, a bridge-like conjugation tube is formed to connect neighboring ...<|separator|>
[61]
Hold the salt: Freshwater origin of primary plastids - PNAS
Aug 31, 2017 · ... the most recent ancestor of Archaeplastida at 2.1 billion y ago (Fig. 1). Thus, plastids evolved from among the early-diverging, small ...
[62]
The Origin and Evolution of the Plant Cell Surface: Algal Integrin ...
Scaly green flagellates (prasinophytes) likely represent the ancestral stock of green algae from which all other green algae (and land plants = embryophytes) ...
[63]
Six newly sequenced chloroplast genomes from prasinophyte green ...
Oct 4, 2014 · Given their basal positions, prasinophytes hold the key to understanding the nature of the last common ancestor of all green plants and the ...
[64]
Large Phylogenomic Data sets Reveal Deep Relationships and Trait ...
The core Chlorophyta is an ancient, species-rich group of photosynthetic eukaryotes that includes the classes Pedinophyceae, Chlorodendrophyceae, and the UTC ...
[65]
The evolution of inorganic carbon concentrating mechanisms in ...
Inorganic carbon concentrating mechanisms (CCMs) catalyse the accumulation of CO 2 around rubisco in all cyanobacteria, most algae and aquatic plants.
[66]
Streptophyte algae and the origin of embryophytes - PMC - NIH
Land plants (embryophytes) evolved from streptophyte green algae, a small group of freshwater algae ranging from scaly, unicellular flagellates (Mesostigma) ...
[67]
[PDF] Sterols in red and green algae: quantification, phylogeny ... - Harvard
Paleobiologists have also used sterane data, in particular the patterns in C29 and C28 steranes, to support fossil evidence of an early radiation of green algae.