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Clavibacter michiganensis

Clavibacter michiganensis is a Gram-positive, catalase-positive, non-spore-forming, non-motile, rod-shaped bacterium belonging to the family Microbacteriaceae in the phylum Actinobacteria, notable for its role as a plant pathogen causing bacterial canker in tomato (Solanum lycopersicum).^[1] The species was originally classified under the genus Corynebacterium but was reclassified into the genus Clavibacter in 1984, with subsequent reclassification of its former subspecies as separate species based on genomic analyses as of 2019.^[2] It is aerobic and proliferates primarily in the xylem tissues of host plants, leading to wilting, cankers, and necrosis.^[3] The pathogen causes bacterial canker of tomato, a seed-borne disease that results in unilateral wilting, stem cankers, leaf spots, and characteristic bird's-eye lesions on fruit—small necrotic spots with white halos.^[3] It can infect all aboveground parts of the plant, with symptoms varying by infection stage and plant age; young seedlings may collapse systemically, while older plants exhibit localized foliar and fruit damage. Transmission occurs primarily through contaminated seeds, irrigation water, tools, and debris, with latent infections capable of harboring up to 10^10 bacteria per gram of tissue.^[2] Related species in the genus include C. sepedonicus, responsible for bacterial ring rot in potato (Solanum tuberosum), characterized by vascular discoloration and tuber rot; C. nebraskensis, causing wilt in maize; and C. insidiosus, leading to bacterial wilt in alfalfa.^[2] These pathogens collectively pose a global threat to solanaceous and leguminous crops, with yield losses from C. michiganensis alone reaching up to 84% in severe outbreaks, underscoring the need for seed certification, sanitation, and integrated management strategies.^[3] Genomic studies reveal a 3.3 Mb chromosome and plasmids encoding virulence factors like proteases and cellulases, which facilitate xylem invasion and host colonization.^[2]

Introduction

General Description

Clavibacter michiganensis is a Gram-positive, aerobic, non-motile, non-sporulating actinomycete bacterium with irregular rod-shaped (coryneform) cells that form curved or club-shaped arrangements.^[4] It produces domed, round, shiny mucoid colonies that are typically yellow-pigmented, though pink variants occur occasionally.^[5] These characteristics enable its proliferation within plant vascular tissues, where it colonizes xylem vessels and disrupts water transport.^[6] The bacterium belongs to the family Microbacteriaceae within the order Micrococcales and features a type B2γ peptidoglycan cell wall crosslinked by diaminobutyrate residues, a trait shared among phytopathogenic coryneform bacteria in the genus.^[7] This cell wall composition contributes to its resilience in diverse environmental conditions, including survival in plant debris for extended periods.^[4] As a primary pathogen, C. michiganensis induces vascular wilt and canker diseases in solanaceous crops, with tomatoes (Solanum lycopersicum) serving as the principal host; infections manifest as unilateral wilting, stem cankers, and bird's eye spots on fruits due to systemic xylem invasion.^[6] It was first described in 1910 by E.F. Smith as Bacterium michiganense, based on isolates from diseased tomato plants in Grand Rapids, Michigan, USA, marking the initial recognition of bacterial canker as a devastating tomato disease.^[5] C. michiganensis exhibits a global distribution across all continents except Antarctica, facilitated by international trade in contaminated tomato seeds.^[5] It is designated as a quarantine pest, appearing on the European and Mediterranean Plant Protection Organization (EPPO) A2 list for regulated non-quarantine pests and regulated by the United States Department of Agriculture (USDA) under plant pest movement restrictions to prevent further spread.^[6]^[8]

Economic and Agricultural Impact

Clavibacter michiganensis subsp. michiganensis causes bacterial canker and wilt in tomatoes, leading to severe yield reductions of 46–93% in affected plants and approximately 50% decreases in average fruit weight during epidemics.^[3] This pathogen, along with related species such as Clavibacter sepedonicus (formerly C. michiganensis subsp. sepedonicus, causing potato ring rot; reclassified in 2018), Clavibacter nebraskensis (formerly subsp. nebraskensis, causing alfalfa wilt), and Clavibacter insidiosus (formerly subsp. insidiosus, causing bacterial wilt in alfalfa), contributes to substantial economic impacts on the tomato, potato, and alfalfa industries through crop destruction and production disruptions.^[9]^[10]^[7] For instance, sporadic epidemics in Michigan, USA, have resulted in losses up to $300,000.^[5] The bacterium's quarantine status amplifies its agricultural impact; C. michiganensis subsp. michiganensis is listed as an A2 quarantine pest by the European and Mediterranean Plant Protection Organization (EPPO), necessitating strict regulatory measures including seed certification and testing in the EU and USA to prevent introduction and spread.^[6] Seedborne transmission facilitates international dissemination, often resulting in bans on contaminated seed lots and restrictions on trade, which further strain global tomato seed markets.^[11] Historical outbreaks underscore the pathogen's threat; major epidemics occurred in European greenhouses during the 1980s, while severe incidents in Iranian tomato fields were reported in 2019, prompting intensified surveillance.^[12]^[13] These events have driven research into resistant tomato varieties, though no fully effective commercial options exist yet, leaving growers reliant on cultural and regulatory controls to mitigate ongoing risks.^[14]

Taxonomy and Classification

Historical Nomenclature

The bacterium responsible for bacterial canker of tomato was first described and named Bacterium michiganense by Erwin F. Smith in 1910, based on isolates from diseased tomato plants in Grand Rapids, Michigan, USA.^[15] This initial classification placed it among the early descriptions of plant-pathogenic bacteria, reflecting the limited taxonomic frameworks available at the time.^[16] In 1934, H.L. Jensen transferred the species to the genus Corynebacterium, renaming it Corynebacterium michiganense due to its coryneform (club-shaped) rod morphology and other phenotypic similarities to established corynebacteria.^[17] This reclassification aligned it with other Gram-positive, irregular-rod bacteria, a grouping that persisted for several decades. By 1977, Dye and Kemp further refined the taxonomy by designating it as Corynebacterium michiganense pv. michiganense to distinguish it from other pathovars within the species, emphasizing host-specific pathogenicity.^[18] In 1982, Carlson and Vidaver elevated it to subspecies status as Corynebacterium michiganensis subsp. michiganensis, incorporating biochemical and serological data to delineate it from related strains.^[19] A pivotal shift occurred in 1984 when Davis et al. established the new genus Clavibacter and transferred the subspecies, naming it Clavibacter michiganensis subsp. michiganensis. This move was driven by chemotaxonomic evidence, particularly the unique presence of 2,4-diaminobutyric acid in the cell wall peptidoglycan, distinguishing it from typical corynebacteria and aligning it with actinomycete-like traits.^[20] Key supporting studies included Collins and Jones (1980), who analyzed cell wall lipids and peptidoglycans in coryneform bacteria, confirming the distinct B2γ variation in these phytopathogens.^[20] Prior to 2015, the species Clavibacter michiganensis encompassed multiple subspecies, including C. michiganensis subsp. sepedonicus (causing ring rot in potato), subsp. nebraskensis (Goss's wilt in corn), subsp. insidiosus (vascular wilt in alfalfa), and subsp. tessellarius (mosaic in wheat), based on host range and phenotypic differences.^[18]

Current Taxonomic Status

As of 2025, Clavibacter michiganensis sensu stricto designates the Gram-positive bacterial pathogen primarily responsible for bacterial canker of tomato, previously classified as Clavibacter michiganensis subsp. michiganensis. This elevation to species status occurred in 2018 following whole-genome sequencing and multi-locus sequence analyses, which demonstrated sufficient genomic divergence to warrant reclassification of several former subspecies as distinct species within the genus Clavibacter.^[21]^[5] The genus Clavibacter belongs to the phylum Actinomycetota, class Actinomycetes, order Micrococcales, and family Microbacteriaceae. Historically, the genus encompassed a single species, C. michiganensis, with nine host-specific subspecies; however, genomic studies have led to the recognition of multiple species, including Clavibacter sepedonicus (causal agent of potato ring rot), Clavibacter nebraskensis (goss's bacterial wilt of corn), and Clavibacter phaseoli (derived from the former subspp. chilensis and phaseoli, affecting beans).^[22]^[23]^[6] Recent taxonomic revisions include the 2022 elevation of Clavibacter michiganensis subsp. californiensis to Clavibacter californiensis sp. nov., based on average nucleotide identity and digital DNA-DNA hybridization analyses of complete genomes, which also merged and reclassified subspp. chilensis and phaseoli into C. phaseoli sp. nov. In 2023, Clavibacter lycopersici sp. nov. was described as a novel, non-motile, peach-colored actinobacterium isolated from asymptomatic tomato plants, distinguished by its 16S rRNA gene sequences and phenotypic traits from other Clavibacter species.^[23]^[24] The type strain of C. michiganensis is DSM 46364 (also known as LMG 7333^T or NCPPB 2979^T), isolated from tomato (Solanum lycopersicum), with the NCBI taxonomy ID 28447. This strain serves as the reference for genomic and phenotypic comparisons in ongoing taxonomic studies.^[25]^[22]

Morphology and Physiology

Cellular Characteristics

Clavibacter michiganensis cells are irregular, short rods measuring approximately 0.4–0.75 μm in width and 0.8–2.5 μm in length, often appearing straight to slightly curved or wedge-shaped, with pleomorphic forms including V, Y, and palisade arrangements particularly evident in older cultures.^[26] The bacterium does not form mycelium or endospores.^[26] As a Gram-positive actinobacterium, it possesses a thick peptidoglycan layer in its cell wall, contributing to its staining properties and structural integrity.^[3] Cells are non-motile and lack flagella.^[26] The bacterium relies on aerobic respiration for metabolism and tests positive for catalase activity while negative for oxidase.^[26] Optimal growth occurs at temperatures of 24–28°C and pH values around 7.0–7.2, conditions that support its proliferation in laboratory media and host environments.^[6] On nutrient agar, colonies develop as small (1–2 mm), circular to irregular, convex, mucoid structures with a glistening appearance, typically exhibiting yellow to orange pigmentation after 3–5 days of incubation at 28°C.^[26] Unlike many Gram-negative phytopathogens, C. michiganensis lacks a type III secretion system for effector delivery.^[27] It produces exopolysaccharides (EPS) that facilitate biofilm formation, aiding adhesion and persistence on plant surfaces and within vascular tissues.^[28]

Growth Requirements

Clavibacter michiganensis exhibits optimal growth at temperatures between 23°C and 28°C, with visible colony formation on agar media typically occurring within 3 to 7 days of incubation under these conditions.^[29] The bacterium can tolerate a broader range of 4°C to 35°C for survival, but active growth ceases below 10°C or above 35°C.^[30] It is heat-sensitive, with viability lost after exposure to 50°C for 30 minutes.^[30] The pathogen prefers a neutral pH range of 7.0 to 8.0 for optimal proliferation, though it demonstrates limited tolerance to acidic conditions, failing to survive beyond 24 hours at pH 4.0 to 4.5 in nutrient solutions.^[31]^[32] As an aerobic actinomycete, C. michiganensis requires oxygen for growth but can persist in low-oxygen environments such as plant xylem vessels during infection.^[33] Nutritionally, the bacterium utilizes carbohydrates like glucose, sucrose, and mannitol as primary carbon sources, supplemented by amino acids and organic acids such as succinate and citrate.^[34]^[35] It grows well on standard media including nutrient agar, yeast extract-mannitol agar, and nutrient broth yeast extract agar (NBYA), often achieving maximum biomass in glucose-enriched broth.^[36]

Genetics and Virulence

Genome Organization

The genome of Clavibacter michiganensis subsp. michiganensis consists of a single circular chromosome and, in pathogenic strains, typically two plasmids that contribute to its genetic architecture and virulence potential.^[37] Genome organization varies among subspecies, with differences in plasmid presence and size; for example, subsp. sepedonicus has two plasmids of approximately 90 kb and 145 kb, while subsp. insidiosus lacks plasmids.^[38] The chromosome measures approximately 3.3 Mb in length, with a G+C content of about 73%, and encodes around 3,000 genes, including essential housekeeping genes for metabolism, replication, and transcription, as well as numerous hypothetical proteins of unknown function.^[37] This compact structure reflects the bacterium's adaptation as a plant pathogen, with a high coding density that supports efficient resource utilization in host environments.^[37] Pathogenic isolates commonly harbor two circular plasmids: pCM1, approximately 27-32 kb in size with a lower G+C content of around 68%, which is essential for virulence and carries the celA gene encoding a cellulase; and pCM2, larger at about 70 kb with a G+C content of roughly 67%, which enhances symptom severity and includes the pat-1 gene for a patatin-like protein.^[37] These plasmids exhibit variability in size and gene content across strains, ranging from 31-59 kb for pCM1-like elements and 64-109 kb for pCM2-like elements, influencing the bacterium's ability to cause disease.^[27] On the chromosome, a pathogenicity island (PAI) of approximately 100-129 kb, characterized by a reduced G+C content (64-67%), integrates regions such as chp (encoding cellulases and hemicellulases) and tomA (encoding a serine protease), which are critical for tissue invasion and symptom development.^[37] As of November 2025, 327 genomes of C. michiganensis had been sequenced and deposited in public databases, revealing low overall genetic diversity with average nucleotide identity (ANI) values exceeding 99% among strains of the same subspecies, indicative of a clonal population structure.^[5]^[39] However, variations in plasmid content and composition contribute to differences in host specificity and pathogenicity across isolates.^[27] Recent analyses in 2025 have highlighted the diversity of genomic islands on these plasmids, such as the 13-kb GIα (containing celA) and 24-kb GIβ (containing pat-1 and related effectors), which determine the progression from canker-like lesions to severe wilting symptoms in hosts like tomato, with isolates lacking these islands uniformly failing to induce wilt.^[40] Notably, C. michiganensis lacks an endogenous CRISPR-Cas system, relying instead on other defense mechanisms against phages and mobile elements.^[41]

Key Virulence Factors

Clavibacter michiganensis, a Gram-positive actinomycete pathogen, relies on a suite of enzymatic and structural factors for virulence rather than the toxin-based effectors or type III secretion systems typical of Gram-negative plant pathogens.^[37] Detailed studies of virulence mechanisms focus primarily on subsp. michiganensis, where pathogenicity involves cell wall degradation, tissue necrosis, and vascular occlusion through exopolysaccharide (EPS) production, enabling systemic spread in host xylem. Other subspecies, such as subsp. sepedonicus, possess distinct virulence genes adapted to their hosts, like those causing ring rot in potato.^[27]^[38] Unlike Gram-negatives, C. michiganensis lacks a type III secretion system and instead secretes enzymes via general secretory pathways to facilitate infection.^[42] A primary virulence factor is the cellulase CelA, encoded on the plasmid pCM1, which degrades β-1,4-glucan linkages in plant cell walls to promote initial entry and colonization. Mutants lacking celA exhibit reduced cellulase activity and fail to induce systemic wilting in tomato, demonstrating its essential role in vascular spread.^[43] The serine protease family encoded by chpA to chpI genes, located within the chromosomal chp/tomA pathogenicity island (PAI), contributes to tissue necrosis and wilting by cleaving plant proteins.^[44] Specifically, ChpG influences host specificity, as allelic variations in chpG modulate recognition by plant immune receptors, restricting virulence in certain tomato cultivars.^[45] The PAI, spanning approximately 129 kb, integrates these proteases to enhance colonization efficiency.^[46] TomA, another key serine protease in the PAI's tomA subdomain, induces a hypersensitive response in non-host plants like Nicotiana species and supports necrosis in tomato.^[47] It is secreted through an unidentified mechanism, independent of type III systems, and mutants show attenuated symptom severity.^[48] EPS production, regulated in part by plasmid-borne genes, enables biofilm formation in xylem vessels, physically blocking water flow and exacerbating wilting.^[49] These polysaccharides, including fucose-glucose-galactose polymers, accumulate during infection to promote bacterial adhesion and persistence.^[50] Recent studies have identified additional factors, including the chromosomal cviA1 gene, which is crucial for necrosis in Nicotiana benthamiana leaves and contributes to mild wilting in tomato.^[51] Transposon mutants of cviA1 display delayed necrosis without affecting canker or blister formation, highlighting its targeted role in symptom development.^[52] Furthermore, two plasmid-borne genomic islands—GIα containing celA and GIβ containing pat-1 (a serine protease)—exhibit genetic diversity across strains and determine whether infections manifest as wilt or localized canker.^[40] Analysis of 88 isolates revealed 11 plasmid variants, with strains lacking one or both islands causing canker but not wilting, as confirmed by complementation assays.^[53] This diversity underscores the modular nature of virulence in C. michiganensis.^[54]

Pathogenicity and Disease

Primary Hosts and Symptoms

Clavibacter michiganensis primarily infects tomato (Solanum lycopersicum) as its main host, causing bacterial canker disease, with secondary hosts including eggplant (Solanum melongena) and other Solanaceae such as wild Solanum species.^[5] Related species in the genus, formerly classified as subspecies of C. michiganensis, affect other crops: Clavibacter sepedonicus (formerly subsp. sepedonicus) causes bacterial ring rot in potato (Solanum tuberosum); Clavibacter capsici (formerly subsp. capsici) causes canker in pepper (Capsicum annuum). Experimental infections have been observed in Nicotiana benthamiana through artificial inoculation.^[5] In tomato, symptoms typically begin with interveinal chlorosis and yellowing on leaves, progressing to necrotic margins and unilateral wilting, often starting from the lower canopy and moving upward.^[55]^[56] Stem cankers develop with tan to brown streaks, internal vascular discoloration, and mealy pith, accompanied by a gummy, yellow exudate when cut surfaces are squeezed.^[55]^[56] On fruits, characteristic "bird's eye" spots appear as small, raised, dark lesions with pale halos, leading to premature ripening and reduced quality.^[55]^[56] On potato, Clavibacter sepedonicus causes bacterial ring rot, characterized by wilting and yellowing of lower leaves with rolled margins, followed by vascular discoloration in stems and tubers forming a ring-like brown necrosis.^[57] Latent infections are common, with tubers showing no external signs but harboring bacteria that ooze as a milky fluid from cut vascular tissue, potentially leading to hollow, cracked structures in storage.^[57] Other related species affect additional hosts: Clavibacter insidiosus (formerly subsp. insidiosus) causes bacterial wilt in alfalfa (Medicago sativa), resulting in stunted growth, yellow-green foliage, mottled and cupped leaflets, and tan to brown lesions on taproots.^[58] Clavibacter nebraskensis (formerly subsp. nebraskensis) induces bacterial stalk rot in corn (Zea mays), with symptoms including wilting, gray-green streaks on leaves, water-soaked lesions with bacterial ooze, and internal stalk softening.^[59] Clavibacter tessellarius (formerly subsp. tessellarius) leads to bacterial mosaic in wheat (Triticum aestivum), manifesting as small, yellow lesions with indefinite margins scattered uniformly, creating a mosaic pattern on leaves.^[60] The incubation period for symptom development is typically 2-4 weeks after infection, though it can vary with plant age and environmental conditions.^[18] Symptom severity differs among strains; for instance, loss of virulence plasmids in Clavibacter michiganensis delays wilting and limits effects to localized hypersensitive responses without systemic disease.^[61] Non-host plants, such as most cereals, show no symptoms unless infected by reclassified species specific to those crops.^[5]

Infection Process and Disease Cycle

The infection process of Clavibacter michiganensis begins with entry into host plants primarily through contaminated seeds, which serve as the main source of primary inoculum, or via mechanical wounds, hydathodes, stomata, and damaged tissues created during cultural practices such as pruning or grafting.^[30] Transmission from seeds to seedlings can occur at rates as low as 0.01% to 85%, with even 5 bacterial cells per seed sufficient to initiate infection under favorable conditions.^[30] Once inside, the bacterium adheres to plant surfaces and penetrates intercellular spaces, particularly beneath stomata or at wound sites.^[62] Following entry, C. michiganensis colonizes the xylem vessels, where it multiplies and forms biofilm-like aggregates, preferentially attaching to the spiral secondary wall thickenings of protoxylem elements.^[63] This colonization spreads systemically in an acropetal (upward) and basipetal (downward) manner through the vascular system, producing extracellular polysaccharides (EPS) that occlude vessels and restrict water flow, leading to wilting.^[62] The bacterium lacks motility genes, relying instead on passive movement within the xylem sap. Enzymatic activities, such as those from cellulases and other degradative enzymes, facilitate tissue invasion and contribute to necrosis during this phase.^[63] Symptom development typically emerges 10 to 42 days post-infection, triggered by vascular occlusion and enzymatic degradation that cause tissue necrosis and systemic wilting.^[62] Bacteria can exit colonized tissues through guttation droplets from hydathodes, enabling secondary spread within the plant canopy via water splash or contact.^[63] Latency periods allow asymptomatic persistence, with infected seedlings harboring up to 3 × 10^6 colony-forming units per gram of tissue for up to 17 days or longer under stress-free conditions, and seeds remaining viable carriers for months.^[62] Overwintering occurs primarily in infected plant debris, where the bacterium survives for 2 months to 2 years depending on environmental conditions, or enters a viable but non-culturable (VBNC) state to endure stress.^[62] Dissemination happens mechanically through contaminated tools, hands, or transplants during pruning and handling, as well as via irrigation water, rain splash, or long-distance seed trade; no confirmed insect vectors are involved.^[30] The full disease cycle spans 1 to 2 years, from primary seed infection and systemic colonization (reaching the apical meristem in about 15 days) through symptom expression, seed contamination in the current season, and survival in debris to initiate the next cycle.^[63] Strain variations, such as those with the pCM1 plasmid, can influence severity, with plasmid-positive strains promoting more aggressive canker formation compared to plasmid-negative ones causing milder wilting.^[62]

Epidemiology and Environmental Factors

Clavibacter michiganensis exhibits a global distribution, with established presence in the Americas (including the United States since its initial detection in 1909, Chile, and Uruguay), Europe (reported in 16 member states of the European Union, such as Italy and Belgium), and Asia (including Iran and other regions). The pathogen has spread through international trade, leading to emerging outbreaks in greenhouse production systems; for instance, multiple introductions in Iran around 2019 were traced to contaminated seed imports, highlighting the role of global commerce in its dissemination. As of February 2025, detections were reported on tomato plants intended for planting in the Netherlands.^[64] While transient populations occur in some areas, persistent infections are more common in intensive tomato-growing regions with favorable climates. The bacterium thrives under warm temperatures of 25–30°C and high humidity, which promote bacterial multiplication, symptom development, and secondary spread within fields or greenhouses. It demonstrates resilience in varied soil conditions, surviving in dry soils for extended periods but proliferating more rapidly in moist environments such as irrigation systems, where water facilitates mechanical transmission. Survival in the environment is notable, with viability persisting for 45–75 days (approximately 2 months) in infected root tissues and soil under greenhouse conditions, and up to 2 years in crop residues depending on exposure and location. Infection thresholds are low, with as few as 10²–10⁴ colony-forming units (CFU) per seed sufficient to establish disease in seedlings. Key risk factors for epidemics include the use of contaminated transplants and seeds, inadequate sanitation practices, and mechanical injury from tools or equipment, which enable local dissemination. International trade has driven multiple introductions, contributing to outbreaks that can reduce fruit yield by 20–85% in severe cases, as observed in various global epidemics. Recent epidemiological studies (2020–2025) indicate low genetic diversity in many strains, suggestive of clonal propagation from single introduction events, which aids rapid establishment but limits adaptability. Non-pathogenic Clavibacter strains, often seed-associated, further complicate detection efforts by interfering with diagnostic assays. Long-distance spread is absent via airborne mechanisms; instead, dissemination relies primarily on anthropogenic pathways, such as infected planting material and contaminated machinery. As of 2025, research has identified diverse plasmid-borne virulence genomic islands that contribute to wilt symptom development in host plants.^[40]

Detection and Management

Diagnostic Methods

Diagnosis of Clavibacter michiganensis relies on a combination of visual inspection, serological assays, culture-based isolation, molecular techniques, and advanced genomic methods to confirm the presence of the pathogen in plants, seeds, and environmental samples. Initial visual assessment often involves observing symptoms such as wilting, cankers, and leaf spots on tomato plants, supplemented by the streaming test, where a milky bacterial ooze exudes from cut stem sections when immersed in water, indicating vascular infection.^[18]^[65] Serological methods, particularly enzyme-linked immunosorbent assay (ELISA), enable rapid antigen detection in seeds and plant tissues. Commercial ELISA kits target specific antibodies against C. michiganensis subsp. michiganensis (Cmm), providing qualitative results for infected material with high specificity for the causal agent of bacterial canker.^[66]^[67] Culture-based detection involves isolating the bacterium from symptomatic tissues or seed extracts on semiselective media, such as CNS (Corynebacterium-Nebraskense Selective) agar supplemented with nalidixic acid to inhibit competing flora. Characteristic yellow, convex colonies appear after 4–7 days at 24–28°C, confirmed by biochemical tests including positive catalase activity, which distinguishes C. michiganensis from related bacteria.^[68]^[26] Molecular techniques provide sensitive and specific identification, with conventional PCR targeting genes such as 16S rRNA for genus-level detection, tomA (encoding tomatinase), and chp (part of the pathogenicity island) for subspecies confirmation. Quantitative PCR (qPCR) enhances quantification, achieving sensitivities down to 1 pg of bacterial DNA or approximately 10² colony-forming units (CFU)/g in seed lots, allowing early detection in asymptomatic material. Loop-mediated isothermal amplification (LAMP) offers field-deployable alternatives, amplifying targets like micA at 65°C without thermal cycling, with results visible via turbidity or colorimetric indicators in under 60 minutes.^[69]^[70]^[71] Advanced methods include whole-genome sequencing (WGS) for strain typing via multilocus sequence typing (MLST) using housekeeping genes such as atpD, dnaK, gyrB, ppk, and recA, enabling phylogenetic differentiation of pathogenic isolates. Immunofluorescence assays localize the bacterium in xylem tissues using fluorescent monoclonal antibodies, aiding histopathological confirmation with sensitivities comparable to PCR.^[44]^[72] Recent advancements as of 2025 incorporate genome-informed multiplex PCR assays, leveraging comparative genomics to target unique markers like rhuM and tomA for distinguishing pathogenic from non-pathogenic Clavibacter strains in mixed infections. Emerging DNA probe-based methods, such as dot blot hybridization targeting tomA, facilitate detection of C. michiganensis subsp. michiganensis in artificially infected tomato seeds down to 4 μg/μL DNA concentration.^[73]^[74]^[75] Quarantine standards, as outlined in the European and Mediterranean Plant Protection Organization (EPPO) PM 7/42 protocol, mandate integrated approaches combining visual inspection, immunofluorescence or ELISA for screening, culture isolation, and PCR confirmation for seed certification and phytosanitary measures, ensuring detection limits suitable for latent infections.^[65]

Control Strategies

Control of Clavibacter michiganensis subsp. michiganensis (Cmm), the causal agent of bacterial canker in tomato, relies on integrated strategies emphasizing prevention, as no curative treatments exist once the pathogen becomes systemic in plants.^[76] Primary approaches include cultural practices to limit introduction and spread, chemical applications to suppress secondary infections, biological agents for antagonism, and breeding for host resistance, often combined in multifaceted programs.^[33] Cultural methods form the foundation of management by reducing pathogen entry and survival. Planting certified pathogen-free seeds is essential, achieved through treatments such as hot-water immersion at 52°C for 20 minutes or 56°C for 30 minutes, which eradicate Cmm from infested seeds while minimizing germination loss to 10-15%.^[77]^[18] Acid extraction with hydrochloric acid also effectively removes surface and internal contamination.^[78] Crop rotation excluding solanaceous hosts like tomatoes, potatoes, peppers, and eggplants for 3-4 years prevents soilborne persistence, as Cmm survives in debris and alternative hosts.^[76]^[79] Early rogueing of infected plants, combined with sanitation to remove debris and avoid composting diseased material, limits spread via tools and irrigation; tools should be disinfected with 10% bleach solutions.^[76] In greenhouses, improving ventilation and using drip irrigation to keep foliage dry further reduces humidity-favored dissemination.^[79] Quarantine measures, including inspection of transplants for lesions, are critical to block introduction.^[80] Chemical controls target foliar bacteria but offer limited efficacy against systemic infections and face challenges from resistance. Copper-based bactericides, such as copper hydroxide applied at 2-4 kg active ingredient per hectare in foliar sprays every 5-7 days, reduce secondary spread by limiting epiphytic populations, particularly when combined with mancozeb.^[81]^[33] Antibiotics like streptomycin (0.25 g active ingredient per liter) provide short-term suppression but are restricted due to emerging resistance and regulatory limits in many regions.^[81]^[33] These are most effective as preventive sprays on seedlings in controlled environments. Biological control employs antagonistic microbes to compete with or inhibit Cmm. Seed treatments with Pseudomonas fluorescens or Bacillus species, such as B. subtilis or B. amyloliquefaciens, induce systemic resistance and reduce disease incidence by 50-70% in greenhouse trials through production of antimicrobial compounds like siderophores and hydrogen cyanide.^[82]^[33] For instance, formulated B. amyloliquefaciens achieved up to 74% wilt reduction, while P. fluorescens cell suspensions yielded 40-67% control.^[82] These agents are applied as seed coatings or soil drenches and promote plant growth alongside pathogen suppression. Host resistance remains partial, with no fully immune commercial varieties available, though breeding programs incorporate traits from wild Solanum species showing reduced symptom severity.^[14] Some hybrids exhibit tolerance to foliar blight, but vascular wilt resistance is limited; genes like those analogous to Ve for other pathogens have proven ineffective against Cmm.^[79] Ongoing efforts focus on marker-assisted selection from resistant accessions.^[83] Integrated pest management combines these tactics for optimal outcomes, emphasizing early detection to enable timely rogueing and sanitation, while avoiding reliance on any single method.^[80] In practice, starting with treated seeds, applying biological seed treatments, following with copper sprays in high-risk settings, and enforcing rotation and quarantine has minimized outbreaks in production systems.^[33] Recent advances as of 2025 include biocontrol with Trichoderma species, such as T. harzianum, which reduced disease severity by 29% in combined foliar spray and soil drench applications.^[33] Genome editing via CRISPR/Cas9 targets host genes for improved resistance; for example, CRISPRa activation of SlPAL2 upregulates phenylpropanoid pathways, enhancing defense and reducing canker symptoms through increased H3K4me3 epigenetic marks.^[84] Similarly, editing SlPR-1 boosts pathogenesis-related gene expression for broader bacterial resistance.^[85] These approaches, alongside bacteriophage therapies like CMP1, promise sustainable, non-chemical alternatives.^[33]

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EPPO has listed C. michiganensis subsp. michiganensis as an A2 quarantine pest. (OEPP/EPPO, 1982), and CPPC and IAPSC also consider it of quarantine ...Missing: global USDA
[12]
[PDF] Pests of tomato crops Plant Pest Factsheet - Plant Health Portal
Tomato bacterial canker (Clavibacter michiganensis ssp. michiganensis) has a global distribution, and there have been many outbreaks within Europe.
[13]
Multiple Introductions of Tomato Pathogen Clavibacter ...
The purposes of the present study were to (i) determine the geographic distribution of the bacterial canker pathogen in tomato-growing areas in Iran and (ii) ...Missing: Antarctica | Show results with:Antarctica
[14]
Wild tomatoes resist devastating bacterial canker - Cornell CALS
Jan 16, 2020 · This is the first study ever confirming that wild tomatoes are susceptible to bacterial canker, though the infection is less severe than in ...
[15]
Clavibacter michiganensis (Smith, 1910) Davis et al. 1984 - GBIF
Clavibacter Davis et al., 1984. Species. Clavibacter michiganensis (Smith, 1910) Davis et al., 1984. = Clavibacter michiganense (Smith, 1910) Davis et al., 1984.Missing: historical nomenclature EF
[16]
Species: Clavibacter michiganensis - LPSN
Name: Clavibacter michiganensis corrig. (Smith 1910) Davis et al. 1984. Category: Species. Proposed as: comb. nov. Basonym: "Bacterium michiganense" Smith 1910.Missing: historical EF<|control11|><|separator|>
[17]
Species: Corynebacterium michiganense - LPSN
❗ Corynebacterium michiganense is the correct name instead if this species is regarded as a separate species (i.e., if its nomenclatural type is not assigned to ...
[18]
Clavibacter michiganensis (bacterial canker of tomato)
Severe infection of tomato plants with the bacterial canker pathogen Clavibacter michiganensis leading to overall wilting and plant death.
[19]
[PDF] Scientific Opinion on the pest categorisation of <em ... - IRIS
Corynebacterium michiganense pv. michiganense (Dye and Kemp, 1977), Corynebacterium michiganense subsp. michiganense (Carlson and Vidaver, 1982). Taxonomic ...
[20]
Clavibacter: a New Genus Containing Some Phytopathogenic ...
Jan 4, 1984 · A new genus containing some phytopathogenic coryneform bacteria, including Clavibacter xyli subsp. xyli sp. nov., subsp. nov. and Clavibacter xyli subsp. ...
[21]
Re-classification of Clavibacter michiganensis subspecies on the ...
Re-classification of Clavibacter michiganensis subspecies on the basis of whole-genome and multi-locus sequence analyses Open Access.
[22]
Clavibacter michiganensis - NCBI
"Pseudomonas michiganensis" (Smith 1910) Stevens 1913 , effective name. "Phytomonas michiganensis" (Smith 1910) Bergey et al. 1923 , effective name.Missing: historical | Show results with:historical
[23]
Elevation of Clavibacter michiganensis subsp. californiensis to ...
Sep 15, 2022 · The Gram-positive genus Clavibacter is currently divided into seven species ( Clavibacter michiganensis , Clavibacter nebraskensis ...
[24]
Clavibacter lycopersici sp. nov.: a peach-colored actinobacterium ...
Sep 21, 2023 · Clavibacter lycopersici sp. nov. is a peach-colored, non-pathogenic actinobacterium isolated from a symptomless tomato plant, and is a new ...
[25]
Clavibacter seminis sp. nov. and Clavibacter quasicaliforniensis sp ...
Mar 27, 2025 · The two new species were non-pathogenic on tomato seedlings, their isolation host, which correlated with the absence of the major virulence-associated genes.
[26]
DSM 46364 - Clavibacter michiganensis - Leibniz Institute DSMZ
Clavibacter michiganensis. Strain designation: S 05, SO5. Other collection no. or WDCM no.: ICPB CM 177,IMET 11518,LMG 7333,NCPPB 2979,PDDCC
[27]
PM 7/42 (2) Clavibacter michiganensis subsp. michiganensis - 2013
Apr 18, 2013 · Cells of C. michiganensis subsp. michiganensis are Gram-positive, non-motile, non-spore-forming, short rods (approximately 0.4–0.75 × 0.8–2.5 ...
[28]
Genomic Analysis of Clavibacter michiganensis Reveals Insight Into ...
Aug 18, 2017 · Clavibacter michiganensis subsp. michiganensis is a gram-positive bacterial pathogen that proliferates in the xylem vessels of tomato, causing bacterial canker ...Missing: taxonomy | Show results with:taxonomy
[29]
Colonization and Movement of GFP-Labeled Clavibacter ...
Aug 18, 2011 · EPS was implicated in biofilm formation and virulence of gram ... Aggregation and biofilm formation by Clavibacter michiganensis subsp.<|separator|>
[30]
Temperature at the early stages of Clavibacter michiganensis subsp ...
Jan 23, 2014 · It was found that a shift in growth temperature from 24–26 to 30–32°C for strain NCPPB382 results in plasmid curing and reduced virulence ( ...
[31]
The Pathogen - APS Journals - American Phytopathological Society
The optimum pH for growth of this bacterium is between 7 and 8, but clavibacter still grows in plant xylem at pH 5 (31). The C. michiganensis subsp.
[32]
[PDF] Identification and Biochemical Characterization of some Strains of ...
Optimal growth and development conditions of Clavibacter michiganensis subsp. michiganensis are temperatures between 20 and 30°C and pH 7- 8 (Sen et al., 2015) ...
[33]
Effects of nutrient solution pH on the survival and transmission of ...
Dec 21, 2001 · In a laboratory experiment, C. m. ssp. michiganensis could not survive for 24 h in nutrient solutions with a pH of 4·0 or 4·5, while 1, 14 ...
[34]
Ecofriendly Control Strategies Against Clavibacter michiganensis ...
Aug 21, 2025 · At the early stages of plant growth, systemic infections can cause unilateral wilting, vascular discoloration, and development of necrosis and ...
[35]
[PDF] PM 7/42 (3) Clavibacter michiganensis subsp. michiganensis
Cell morphology. Cells of C. michiganensis subsp. michiganensis are Gram-positive, non-motile, non-spore- forming, short rods (about 0.4–0.75 9 0.8–2.5 μm) ...
[36]
[PDF] A Semiselective Agar Medium for Isolation of Clavibacter ...
Important selective components of this nutrient agar, yeast extract, and salts medium are mannitol, poly- myxin B-sulfate, nalidixic acid, and cycloheximide. ...Missing: YEMA | Show results with:YEMA
[37]
Suppressive Effect of Bioactive Extracts of Bacillus sp. H8-1 ... - NIH
Feb 9, 2022 · Functional characterization of two cellulase genes in the Gram-positive pathogenic bacterium Clavibacter michiganensis for wilting in tomato.
[38]
Survival of Clavibacter michiganensis subsp. michiganensis in ...
Aug 19, 2015 · In host debris left on the soil surface, bacteria survived 120–260 days for crop production cycles that ended in winter and 45–75 days for those that ended in ...
[39]
Isolation of Clavibacter michiganensis subsp. michiganenesis ...
Jul 19, 2023 · Clavibacter michiganensis subsp. michiganensis (Cmm) is causing wilt and canker and significant economic losses in tomato production.
[40]
[PDF] Local Sources of Clavibacter michiganensis ssp. michiganensis in ...
Seed transmission, overwinter survival, and dispersal of Clavibacter michiganensis ssp. michiganensis on processing tomatoes (Lycopersicon esculentum) and ...
[41]
The Genome Sequence of the Tomato-Pathogenic Actinomycete ...
The two circular plasmids, pCM1 (27.4 kbp) and pCM2 (70.0 kbp), which carry pathogenicity genes and thus are essential for virulence, have lower G+C contents ( ...
[42]
Two plasmid‐borne virulence genomic islands of Clavibacter ...
Jun 27, 2025 · Two plasmid-borne virulence genomic islands of Clavibacter michiganensis are genetically diverse and determine the development of wilt symptoms in host plants.
[43]
Exploring Viral Interactions in Clavibacter Species: In Silico Analysis ...
Unlike other bacteria, Clavibacter do not possess an endogenous CRISPR-cas consistent with previous reports [83]. Instead, other defense systems such as PD- ...
[44]
(PDF) Genomic Analysis of Clavibacter michiganensis Reveals ...
Aug 7, 2025 · A large number of secreted Cmm proteins were Carbohydrate-active enzymes (CAZymes). Glycome profiling revealed that Cmm, but not endophytic ...
[45]
Analyses of Virulence Genes of Clavibacter michiganensis subsp ...
The aim of this study was the characterization of nine Cmm strains isolated in Chile to reveal the causes of their differences in virulence.
[46]
Comparative Genomics of Pathogenic Clavibacter michiganensis ...
Genes associated with virulence are located on the chromosome and on two circular plasmids, pCM1 (27.5 kb) and pCM2 (70 kb). Even with the prevalence and ...
[47]
Immune recognition of the secreted serine protease ChpG restricts ...
Apr 19, 2022 · Bacterial wilt and canker caused by Clavibacter michiganensis (Cm) inflict considerable damage in tomato-growing regions around the world.
[48]
Allelic variations in the chpG effector gene within Clavibacter ...
Here, we investigated the host range of the tomato pathogen Clavibacter michiganensis (Cm). We determined the genome sequences of 40 tomato Cm isolates and ...
[49]
Putative Serine Protease Effectors of Clavibacter michiganensis ...
Sep 29, 2015 · The genomes of both subspecies encode members of the pat-1 family of putative serine proteases known to function in virulence on host plants and ...
[50]
Comparative Genome Analyses of Clavibacter michiganensis Type ...
Jan 31, 2022 · Clavibacter michiganensis, a Gram-positive, plant-pathogenic bacterium belonging to Actinobacteria, is a causal agent of bacterial canker in ...
[51]
Deletion of pbpC Enhances Bacterial Pathogenicity on Tomato by ...
Mar 10, 2023 · Clavibacter michiganensis (Cm) is the causal agent of tomato bacterial canker and wilt disease, which results in substantial yield losses and ...
[52]
Exopolysaccharides in the pathogenic interaction of Clavibacter ...
Strains of Group I efficiently colonize tomato plants and produce an EPS with an identical carbohydrate composition with fucose, glucose, galactose.Missing: regulation | Show results with:regulation
[53]
A novel virulence gene, cviA1 of Clavibacter michiganensis for ...
Clavibacter michiganensis is a Gram-positive bacterium that causes diverse disease symptoms in tomatoes and Nicotiana benthamiana, a surrogate host plant ...
[54]
A novel virulence gene, cviA1 of Clavibacter michiganensis for ...
The cviA1 gene of Clavibacter michiganensis is a virulence gene important for necrosis development in Nicotiana benthamiana leaves.
[55]
Two plasmid‐borne virulence genomic islands of Clavibacter ... - PMC
Jun 27, 2025 · The gram‐positive bacterium Clavibacter michiganensis carries two plasmid‐borne important virulence genes, celA and pat‐1, necessary for ...
[56]
Two plasmid-borne virulence genomic islands of Clavibacter ...
Jun 27, 2025 · The gram-positive bacterium Clavibacter michiganensis carries two plasmid-borne important virulence genes, celA and pat-1, necessary for wilting in tomato.Missing: canker | Show results with:canker
[57]
Bacterial Canker of Tomato | NC State Extension Publications
Jan 9, 2024 · Tomato is the primary host of C. michiganensis, but other members of the Solanaceae family (pepper, eggplant, potato, tobacco) may also be hosts ...
[58]
Bacterial Canker of Tomato | Minnesota Department of Agriculture
Bacterial canker of tomato is caused by Clavibacter michiganensis subsp. michiganensis (Cmm). This plant pathogenic bacteria can be introduced to a new area ...
[59]
Bacterial Ring Rot / Potato / Agriculture - UC IPM
Bacterial ring rot causes yellowing leaves, brown necrosis in tubers, and hollow shells. It overwinters in tubers and spreads via cut seed. Use certified seed ...
[60]
Bacterial Wilt / Alfalfa / Agriculture - UC IPM
Symptoms of bacterial wilt include a yellow-green foliage and stunted growth. Leaflets may be mottled and slightly cupped or curled upward.Missing: michiganensis | Show results with:michiganensis
[61]
Goss's bacterial wilt and leaf blight on corn | UMN Extension
Goss's wilt, caused by a bacterial pathogen, infects wounded leaves, causing tan lesions with dark spots and bacterial ooze. It can cause large yield losses.
[62]
Additional Gram-positive Bacterial Diseases in Nebraska Crop ...
May 11, 2021 · The disease was characterized by small, yellow lesions with undefined margins densely and uniformly scattered over entire leaf surfaces, and ...
[63]
Evidence for plasmid-encoded virulence factors in the ... - NIH
The tomato pathogen Clavibacter michiganensis subsp. michiganensis NCPPB382, which causes bacterial wilt, harbors two plasmids pCM1 (27.5 kb) and pCM2 (72 kb).Missing: regulation | Show results with:regulation
[64]
Clavibacter michiganensis Reframed: The Story of How the Genomics Era Made a New Face for an Old Enemy
Summary of each segment:
[65]
Bacterial Canker of Tomato: Revisiting a Global and Economically Damaging Seedborne Pathogen | Plant Disease
### Summary of Clavibacter michiganensis Infection Process and Disease Cycle
[66]
PM 7/42 (3) Clavibacter michiganensis subsp. michiganensis
Jul 19, 2016 · This document describes a diagnostic protocol for Clavibacter michiganensis subsp. michiganensis, a pathogen causing bacterial canker of tomato ...Introduction · Detection · Identification · Appendix 3: Recommended...
[67]
ELISA Reagent Set for Clavibacter michiganensis subsp ... - Agdia
This ELISA test is a qualitative serological assay for the detection of Clavibacter michiganensis subsp. michiganensis (Cmm), the causal agent of bacterial ...
[68]
Clavibacter michiganensis pv. michiganensis (Cmm) ELISA Kit
Rating 5.0 (1) The test can be used to detect Cmm, the causal agent of bacterial canker of tomato, in infected host plants as well as in pure bacterial cultures.
[69]
A New Selective Medium for Isolation of Clavibacter michiganensis ...
A new selective and highly sensitive medium was developed for isolation of Clavibacter michiganensis subsp. michiganensis (Cmm), the.
[70]
Multiplex real-time PCR for the detection of Clavibacter ...
Jan 7, 2020 · A multiplex real-time PCR method based on fluorescent TaqMan® probes was developed for the simultaneous detection of the tomato pathogenic bacteria.
[71]
A new multiplex TaqMan qPCR targeting Clavibacter michiganensis ...
Jun 20, 2023 · Specific primers and hydrolysis probes were designed for the detection of Cm using as templates the sequence of the tomatinase (tomA) gene, the ...
[72]
Loop-mediated amplification of the Clavibacter michiganensis subsp ...
The LAMP reaction identified all C. michiganensis subsp. michiganensis strains and discriminated them from other C. michiganensis subspecies and non-Clavibacter ...
[73]
Detection of Clavibacter michiganensis ssp. michiganensis in ...
The method is based on rapid screening of tomato seed lots using indirect immunofluorescence staining (IF), followed by dilution plating of IF positive seed ...
[74]
A New Multiplex TaqMan qPCR for Precise Detection and ...
Jul 15, 2024 · Multiplex real-time PCR for the detection of Clavibacter michiganensis subsp. michiganensis, Pseudomonas syringae pv. tomato and pathogenic ...
[75]
Comparative Genomics to Develop a Specific Multiplex PCR Assay ...
Jan 31, 2020 · The multiplex PCR assay specifically detected C. michiganensis strains from a panel of 110 additional bacteria, including other Clavibacter spp.
[76]
[PDF] DNA Probe as Biosensor Candidate for Clavibacter michiganensis ...
Clavibacter michiganensis subsp. michiganensis is known as the causal agent of bacterial canker in tomato plants. This bacterium is a seed-borne pathogen and ...
[77]
[PDF] Bacterial Canker Disease of Tomato - MU Extension
4. Chemical control. Applications of fixed copper plus mancozeb may reduce secondary spread of the disease.
[78]
Effect of Temperature Treatments on the Viability of Clavibacter ...
Feb 13, 2021 · The objective of the present study was to establish whether exposure to temperatures of 55-70 °C results in eradication of the pathogen Clavibacter sepedonicus ...
[79]
Clavibacter michiganensis: 7 Ways to Control Disease - Farmonaut
How does bacterial wilt in tomatoes differ from ring rot in potatoes? Bacterial wilt causes wilting, vascular discoloration, and cankers in tomatoes. Ring rot ...<|separator|>
[80]
Bacterial canker of tomato | UMN Extension
The disease is caused by the bacterial pathogen Clavibacter michiganensis subspecies michiganensis. Warm temperatures (75 to 90°F), high moisture or relative ...
[81]
Managing Bacterial Canker in Tomato: Key Strategies
Bacterial canker (Clavibacter michiganensis pv michiganensis) is one of the most destructive and puzzling tomato diseases in Massachusetts ... yield losses were ...Missing: percentage | Show results with:percentage
[82]
Limiting Populations and Spread of Clavibacter michiganensis ...
By focusing control strategies in the greenhouse rather than in the field, growers may have better control of the pathogen and avoid economic losses result ...
[83]
Biological control of the tomato wilt caused by Clavibacter ...
Aug 20, 2019 · The present in vitro study and greenhouse experiment aimed to evaluate the efficiency of Bacillus subtilis, B. amyloliquefaciens, Pseudomonas fluorescens, and ...
[84]
QTL-Seq Analysis for Identification of Resistance Loci to Bacterial ...
Jan 26, 2022 · Bacterial canker of tomato (Solanum lycopersicum) is a destructive disease caused by a Gram-positive actinomycete Clavibacter michiganensis (Cm) ...
[85]
CRISPR/dCas12a-mediated activation of SlPAL2 enhances tomato ...
CRISPRa-edited plants demonstrated increased deposition of the H3K4me3 epigenetic mark and significantly upregulated SlPAL2 expression.
[86]
CRISPRa-mediated transcriptional activation of the SlPR-1 gene in ...
Oct 16, 2025 · We provide evidence that edited tomato plants for the PATHOGENESIS-RELATED GENE 1 gene (SlPR-1) show enhanced disease resistance to Clavibacter ...