Fact-checked by Grok 2 weeks ago

Clusterin

Clusterin, also known as apolipoprotein J (ApoJ), is a multifunctional heterodimeric glycoprotein encoded by the CLU gene on human chromosome 8p21, consisting of α and β subunits linked by five disulfide bonds and heavily glycosylated at six N-linked sites, resulting in a secreted molecular weight of 70-80 kDa.^[1]^[2]^[3] It exists in multiple isoforms—secreted (sCLU), cytoplasmic (cCLU), and nuclear (nCLU)—each with distinct functions, primarily acting as an extracellular chaperone that binds misfolded proteins to prevent aggregation, facilitates lipid transport as a component of high-density lipoprotein (HDL), and modulates complement activation to protect cells from lysis.^[1]^[2]^[3] Structurally, human clusterin features a discontinuous three-domain architecture: a long coiled-coil domain (~85 Å) formed by amphipathic helices, a disulfide-rich domain containing all five interchain disulfide bonds, and an α/β roll-like domain with a three-stranded β-sheet, complemented by disordered hydrophobic tails on each subunit that are essential for chaperone activity and receptor binding.^[1] These tails enable sCLU to interact with hydrophobic regions of partially unfolded proteins, inhibiting aggregation of pathological entities like amyloid-β, tau, and α-synuclein, while also supporting endocytosis via receptors such as VLDLR (with a dissociation constant K_D ~80 nM).^[1] The protein's high glycosylation (up to 30% of mass) enhances solubility and stability in extracellular fluids, where plasma concentrations range from 35-105 μg/mL and cerebrospinal fluid levels from 1.2-3.6 μg/mL.^[2]^[3] Functionally, sCLU serves as a cytoprotective chaperone in stress responses, clearing cellular debris during tissue remodeling and injury, while cCLU stabilizes mitochondria to inhibit apoptosis and nCLU promotes cell death by sequestering Ku70 and impairing DNA repair.^[2]^[3] Clusterin is ubiquitously expressed across tissues, with upregulation in processes like mammary gland involution, wound healing, and neurodegeneration, and it plays pivotal roles in disease: as a genetic risk factor for Alzheimer's disease via impaired amyloid clearance, in cancer progression (e.g., anti-apoptotic effects in lung and prostate tumors), cardiovascular pathologies through lipid dysregulation, and fibrosis by modulating collagen deposition.^[4]^[2]^[3] Its therapeutic potential includes targeting sCLU for enhancing protein clearance in neurodegenerative disorders or inhibiting it to sensitize tumors to chemotherapy.^[2]^[5]

Genetics and Structure

Gene Organization

The CLU gene, encoding clusterin, is located on the short arm of human chromosome 8 at cytogenetic band 8p21.1, spanning approximately 18 kb from genomic coordinates 27,596,917 to 27,614,700 (GRCh38).^[6] The gene consists of 9 exons ranging in size from 46 to 1,334 bp, interrupted by 8 introns, with the first exon being non-coding.^[7] This organization allows for the production of multiple transcripts through alternative splicing mechanisms. The primary transcript of the CLU gene yields a mature mRNA of approximately 2.3 kb (NM_001831.4), which is translated into the secreted isoform of clusterin (sCLU).^[8] Alternative splicing generates distinct isoforms, including the nuclear form (nCLU), which skips exon 2 to remove the signal sequence coding region, with translation initiating at a downstream AUG codon in exon 3, resulting in a ~49 kDa non-secreted protein. These isoforms share exons 3–9 but differ in their 5' regions due to alternative exon 1 usage or splicing, enabling differential cellular localization and function.^[9]^[10]^[4] A key genetic variant in the CLU gene is the single nucleotide polymorphism (SNP) rs11136000, located in intron 1, where the minor C allele is associated with reduced risk of late-onset Alzheimer's disease (LOAD) by approximately 15–20% in Caucasian populations.^[11] This SNP influences CLU expression levels, with the protective C allele correlating with higher circulating clusterin concentrations, potentially enhancing amyloid-beta clearance.^[12] The promoter region of the CLU gene, spanning upstream of exon 1, contains conserved regulatory elements that drive tissue-specific transcription, including binding sites for hypoxia-inducible factor-1α (HIF-1α) at three hypoxia response elements (HREs) and motifs for transcription factors Sp1/Sp3 and AP-1.^[13] These elements respond to stress signals, such as hypoxia and inflammation, modulating CLU expression; for instance, HIF-1α binding under low oxygen conditions upregulates the nuclear isoform.^[14] Epigenetic modifications, including promoter methylation, further fine-tune transcription in response to cellular cues.^[4] The CLU gene exhibits strong evolutionary conservation across mammals, with orthologs identified in over 200 species, including rodents (e.g., Clu in Rattus norvegicus) and primates (e.g., in Macaca mulatta).^[15] The core exon-intron structure and promoter elements are particularly preserved, reflecting the protein's fundamental roles in proteostasis and stress response since early mammalian divergence.^[16]

Protein Architecture

Clusterin exists primarily as two major isoforms: the secreted form (sCLU) and the nuclear form (nCLU), with a cytoplasmic form (cCLU) also reported. The sCLU isoform is synthesized as a ~52 kDa precursor polypeptide (calculated) that undergoes post-translational processing, including cleavage into α- and β-chains and linkage by five conserved interchain disulfide bonds to form an antiparallel heterodimer.^[1]^[17] This mature unglycosylated structure weighs approximately 48 kDa, but extensive N-linked glycosylation increases the apparent molecular weight to 75-80 kDa, with carbohydrates comprising about 30% of the total mass.^[18]^[17] Recent structural studies have elucidated the architecture of sCLU at high resolution, revealing a three-domain organization consisting of a coiled-coil domain, a disulfide-rich helical domain, and an α/β roll-like domain, with flexible hydrophobic tails at the chain termini.^[1] This crystal structure, resolved at 2.8 Å, highlights amphipathic α-helices, particularly helix α7, which facilitate lipid binding by interacting with hydrophobic tails of phospholipids to form lipoprotein particles.^[1] These helical elements enable sCLU's amphipathic properties, allowing association with diverse lipid species in extracellular environments.^[19] In contrast, the nuclear isoform nCLU is a cytosolic protein lacking the signal peptide for secretion, arising from alternative splicing that skips exon 2, followed by translation initiation at a downstream AUG codon, which results in a distinct N-terminal sequence.^[9] This non-glycosylated form has a molecular weight of approximately 49 kDa and remains primarily in the cytoplasm before potential nuclear translocation under stress conditions.^[20]^[21] The cytoplasmic isoform cCLU, produced from an alternative exon 1 variant, is also ~49 kDa, non-glycosylated, and localized to the cytoplasm without nuclear translocation signals.^[2] Glycosylation of sCLU occurs at six conserved N-linked sites on asparagine (Asn) residues—specifically Asn86, Asn103, Asn145, Asn291, Asn354, and Asn374—predominantly with complex biantennary glycans that vary in sialylation levels across tissues and physiological states.^[22]^[18] This variability in sialylation and glycan composition modulates the protein's molecular weight, with hypersialylated forms appearing larger on gels, and influences conformational stability by preventing aggregation while promoting efficient endoplasmic reticulum exit and secretion.^[18]^[23]

Expression and Regulation

Tissue Distribution

Clusterin (CLU) is ubiquitously expressed across human tissues, with particularly high levels observed in the brain, liver, testis, ovary, and pancreas. In the brain, expression is prominent in astrocytes, contributing to its baseline physiological presence in the central nervous system, with lower expression in neurons. The liver also exhibits elevated expression, reflecting clusterin's roles in systemic processes, while the testis and ovary show high levels in reproductive tissues. Moderate expression is noted in the heart, lung, and kidney, indicating a broader but less intense distribution in cardiovascular, respiratory, and renal systems.^[24]^[2]^[25]^[17] The secreted form of clusterin predominates in extracellular fluids, such as serum and cerebrospinal fluid (CSF), where it circulates at concentrations of approximately 35-105 μg/mL in healthy adults, with typical ranges around 50-100 μg/mL varying by age and sex. In contrast, the nuclear form is primarily intracellular and becomes upregulated in response to cellular stress. CSF levels are notably lower, ranging from 1.2-3.6 μg/mL, underscoring the protein's differential distribution in central versus peripheral compartments.^[2] Developmentally, clusterin expression is low in fetal tissues and increases progressively from gestation to adulthood, establishing higher baseline levels in mature organs. This pattern is evident in various tissues, including the brain and reproductive organs. Hormonal influences, such as androgen regulation, further modulate expression; for instance, androgens repress clusterin in normal prostate tissue, highlighting tissue-specific temporal dynamics.^[16]

Regulatory Mechanisms

The expression of the clusterin gene (CLU) is primarily regulated at the transcriptional level through binding of key transcription factors to its promoter region. Specificity protein 1 (SP1) and activator protein 1 (AP-1) bind to specific sites in the CLU promoter, facilitating basal expression and responses to cellular stress, such as during corneal wound healing where their activity represses CLU during tissue repair.^[26] Nuclear factor kappa B (NF-κB) also interacts with the promoter to induce CLU expression in response to inflammatory signals and proteotoxic stress, contributing to its cytoprotective roles.^[27] Additionally, the presence of heat shock elements (HSE) in the promoter renders CLU stress-inducible, allowing rapid upregulation following heat shock or other proteotoxic insults via heat shock factor 1 (HSF1) activation.^[28] Post-transcriptional mechanisms further fine-tune CLU expression, including microRNA (miRNA)-mediated suppression and alternative splicing. For instance, miR-21 directly targets the CLU transcript, reducing its expression and promoting cell proliferation in head and neck squamous cell carcinoma, where miR-21 overexpression downregulates the growth-suppressive CLU-1 isoform.^[29] Alternative splicing of CLU pre-mRNA generates distinct isoforms, such as the secreted form (sCLU) and nuclear form (nCLU), with regulation influenced by genetic variants like the Alzheimer's disease-associated rs7982 in exon 5, which alters splicing efficiency and isoform ratios in brain tissue.^[30] Environmental cues play a critical role in modulating CLU expression through signaling pathways responsive to stress. Oxidative stress upregulates CLU in various cell types, including airway epithelial cells, to mitigate reactive oxygen species damage and support cellular survival.^[31] Inflammation induces CLU via cytokines like interleukin-6 (IL-6), which enhances its expression in chondrocytes and other tissues to dampen inflammatory responses.^[32] Hypoxia similarly elevates CLU levels through hypoxia-inducible factor pathways, promoting adaptation to low-oxygen environments.^[16] These cues often intersect with feedback loops, such as negative regulation by CLU itself on transforming growth factor beta (TGF-β) signaling, where CLU induction by TGF-β1 limits excessive fibrotic responses in ligamentum flavum cells.^[33] Epigenetic modifications provide another layer of control, particularly in pathological contexts. Hypermethylation of the CLU promoter in cancer cells, such as those in breast, ovarian, and hormone-refractory prostate tumors, silences gene expression and correlates with reduced protein levels, facilitating tumor progression.^[34] This methylation pattern is reversible and responsive to demethylating agents, highlighting its role in dynamically regulating CLU during oncogenesis.^[35]

Biological Functions

Chaperone and Proteostasis Roles

Clusterin functions as an ATP-independent molecular chaperone, primarily in the extracellular space, where it acts as a "holdase" to bind and stabilize misfolded proteins without refolding them, thereby preventing their aggregation into toxic structures.^[1] This activity is crucial for maintaining proteostasis, the cellular and organismal balance of protein folding, trafficking, and degradation, particularly under stress conditions that promote protein misfolding.^[36] For instance, clusterin binds to amyloid-β (Aβ) peptides, a hallmark of Alzheimer's disease, inhibiting their fibrillization and amorphous aggregation by sequestering exposed hydrophobic regions.^[2] The structural basis of clusterin's chaperone activity involves its flexible, hydrophobic tails (residues 204–244) and amphipathic α-helices, such as α7 (residues 244–257), which capture hydrophobic patches on client proteins through non-specific interactions.^[1] These elements form soluble complexes with misfolded substrates, like denatured rhodanese or Aβ, without requiring energy input, distinguishing clusterin from intracellular ATP-dependent chaperones.^[1] Rather than promoting refolding, this binding stabilizes proteins in a degradation-competent state, facilitating their subsequent clearance and avoiding intracellular accumulation that could overwhelm proteasomal pathways.^[36] In proteostasis, clusterin plays a key role in clearing extracellular aggregates from cerebrospinal fluid (CSF, concentrations 1.2–3.6 μg/mL) and plasma (35–105 μg/mL), where it targets fibrillar and prefibrillar structures associated with aging and neurodegeneration.^[2] It promotes lysosomal degradation through receptor-mediated endocytosis, primarily via low-density lipoprotein receptor-related protein 2 (LRP2/megalin), which internalizes clusterin-substrate complexes for breakdown in lysosomes.^[2] This process inhibits proteasome overload by diverting misfolded proteins away from intracellular routes, as evidenced in stress-induced models where clusterin reduces aggregate burden and supports autophagic flux.^[36] Recent 2025 structural studies confirm clusterin's binding to fibrillar Aβ and tau species in aging contexts, underscoring its protective role in extracellular quality control.^[1]

Transport and Adhesion Functions

Clusterin, also known as apolipoprotein J (APOJ), plays a critical role in lipid transport by associating with high-density lipoprotein (HDL) particles to facilitate reverse cholesterol transport (RCT), the process by which excess cholesterol is removed from peripheral tissues and delivered to the liver for excretion.^[37] As a component of a subset of HDL known as J-HDL, clusterin promotes cholesterol efflux from macrophages and other cells, thereby reducing the risk of atherosclerosis by preventing lipid accumulation in arterial walls.^[25] This function is supported by its ability to bind various lipids, including phospholipids and sphingolipids, which enables clusterin to stabilize lipid particles and protect against peroxidation during transport.^[38] In addition to its lipid-handling capabilities, clusterin contributes to cell adhesion and matrix interactions by binding to extracellular matrix (ECM) components such as heparan sulfate proteoglycans.^[2] These interactions modulate epithelial cell migration and adhesion, helping to maintain tissue integrity and regulate cellular movement across ECM barriers without promoting excessive invasion.^[2] Clusterin also facilitates lipid shuttling across physiological barriers, such as the blood-brain barrier (BBB), where it binds to receptors like LRP1 to transport lipids and clear aggregated proteins, including amyloid-β, thereby supporting neuronal homeostasis.^[39] In this context, clusterin contributes to HDL remodeling by stabilizing particle composition, which enhances overall lipid flux without direct enzymatic inhibition of cholesteryl ester transfer protein (CETP).^[40] In reproductive physiology, clusterin is essential for spermatogenesis, where it is secreted by Sertoli cells and deposited on maturing spermatozoa to deliver lipids necessary for membrane stabilization and motility.^[41] Low seminal levels of clusterin correlate with impaired spermatogenesis and reduced fertility, highlighting its role in lipid provision to developing sperm.^[42] Similarly, in ovarian follicle development, clusterin is produced by granulosa cells and serves as a marker for follicular maturation and atresia; its expression patterns align with phases of lipid-dependent growth and resorption in follicles.^[43]

Apoptosis and Cell Survival

Clusterin exhibits a dual role in apoptosis and cell survival through its two main isoforms: the secreted form (sCLU), which predominantly exerts anti-apoptotic effects, and the nuclear form (nCLU), which promotes programmed cell death.^[16] This bifunctionality allows clusterin to fine-tune cellular responses to stress, balancing survival and death pathways.^[44] The anti-apoptotic activity of sCLU primarily involves inhibition of BAX/BAK activation and mitochondrial stabilization. sCLU binds to and stabilizes the Ku70-Bax protein complex in the cytosol, preventing Bax translocation to mitochondria and subsequent cytochrome c release, which reduces caspase activation and apoptosis by approximately fourfold in depleted cells.^[45] Additionally, sCLU activates the PI3K/Akt pathway, often via interaction with megalin, leading to Akt phosphorylation and Bad inactivation, thereby enhancing cell survival in prostate cancer cells exposed to TNFα and actinomycin D.^[46] In inflammatory contexts, sCLU promotes survival by facilitating NF-κB nuclear translocation through ubiquitination and degradation of COMMD1 and IκB, supporting cytoprotection.^[47] In contrast, nCLU drives pro-apoptotic signaling by disrupting the Ku70-Bax complex and promoting DNA damage responses in stressed nuclei. Upon nuclear translocation, nCLU binds Ku70, releasing Bax to activate mitochondrial apoptosis pathways, including caspase-3 activation and cytochrome c release.^[44] nCLU also sequesters Bcl-XL via its BH3 domain, further liberating Bax and amplifying cell death signals in response to stressors like ionizing radiation.^[48] This isoform enhances DNA damage-induced apoptosis by inhibiting repair mechanisms and upregulating pro-death pathways in nuclear compartments.^[20] Under cellular stress, an isoform switch from sCLU to nCLU occurs via alternative splicing, bypassing the ER signal peptide to enable cytosolic retention and nuclear translocation of nCLU.^[16] This shift is regulated by stress signals that alter CLU mRNA processing, as briefly noted in mechanisms of isoform expression. In specific contexts, sCLU protects neurons from oxidative stress by stabilizing mitochondria and activating survival pathways, while in cancer cells, it enhances viability during chemotherapy exposure, such as oxaliplatin treatment in hepatocellular carcinoma, by bolstering PI3K/Akt signaling.^[16]

Protein Interactions

Molecular Partners

Clusterin, also known as apolipoprotein J (ApoJ), interacts with a variety of protein ligands, particularly misfolded or aggregated forms implicated in neurodegenerative processes. It binds amyloid-β (Aβ) oligomers, such as Aβ1–42, with high affinity, forming stable complexes that inhibit further aggregation; the dissociation constant (Kd) for these interactions is in the low nanomolar range, approximately 1 nM.^[49] Similarly, clusterin associates with tau protein aggregates and α-synuclein fibrils, modulating their propagation and toxicity. These interactions often exhibit 1:1 stoichiometry, allowing clusterin to act as an extracellular chaperone stabilizing hydrophobic regions of the ligands.^[50]^[51]^[52] In addition to protein partners, clusterin engages lipid molecules, particularly within high-density lipoprotein (HDL) particles where it serves as a structural component. It binds phosphatidylcholine and cholesterol, facilitating lipid efflux from cells and incorporation into HDL for reverse cholesterol transport; these associations stabilize the lipoprotein structure and promote cholesterol homeostasis. Clusterin also interacts with low-density lipoprotein receptor-related proteins (LRP1 and LRP2), which mediate its endocytosis; binding to LRP2, for instance, enhances uptake of clusterin-ligand complexes into cells.^[37]^[53]^[54] Within the complement system, clusterin directly binds the terminal components of the membrane attack complex (MAC), specifically C5b-7, C8, and C9, to inhibit assembly of the lytic C5b-9 complex on cell surfaces. This binding, which occurs with high affinity and 1:1 stoichiometry per complex subunit, solubilizes the MAC and prevents membrane perforation, thereby protecting host cells from complement-mediated damage.^[55]^[56] Clusterin further modulates signaling pathways by binding other growth factors and their receptors. It associates with transforming growth factor-β (TGF-β) type I and II receptors, altering downstream Smad signaling and epithelial-mesenchymal transition in a ligand-dependent manner, often at 1:1 ratios. Likewise, clusterin binds vascular endothelial growth factor (VEGF), co-localizing with it to influence angiogenesis and endothelial cell responses.^[57]^[58]^[59]

Functional Complexes

Clusterin, also known as apolipoprotein J (ApoJ), forms functional complexes within high-density lipoprotein (HDL) particles, associating with apolipoprotein A-I (APOA1) and lecithin-cholesterol acyltransferase (LCAT) to mediate cholesterol efflux from macrophage foam cells. This HDL-associated complex enhances reverse cholesterol transport by promoting the unloading of excess cholesterol from arterial walls, thereby integrating into anti-atherogenic pathways that mitigate plaque formation and vascular inflammation. Low clusterin levels in HDL have been linked to increased coronary artery disease risk, underscoring its role in maintaining lipid homeostasis during atherosclerosis progression.^[37]^[60] In chaperone-receptor complexes, secretory clusterin (sCLU) forms a triad with amyloid-β (Aβ) and low-density lipoprotein receptor-related protein 2 (LRP2) to facilitate neuronal clearance of Aβ aggregates across the blood-brain barrier. This multi-component assembly binds Aβ with high affinity, enabling LRP2-mediated endocytosis and transport from brain interstitial fluid to systemic circulation, thus preventing toxic accumulation in neuronal environments. The sCLU-Aβ-LRP2 complex represents a key neuroprotective mechanism, with disruptions linked to impaired Aβ disposal in neurodegenerative contexts.^[61]^[4]^[62] Nuclear clusterin (nCLU) participates in signaling hubs involving Ku70 and Bax, modulating DNA repair and apoptosis pathways. nCLU binds Ku70, a DNA double-strand break repair factor, displacing Bax and promoting its translocation to mitochondria to initiate caspase-dependent apoptosis under cellular stress. This interaction shifts Ku70 from its anti-apoptotic cytosolic role to facilitate programmed cell death, while nCLU may also influence DNA repair fidelity by localizing to nuclear damage sites. Additionally, clusterin inhibits NF-κB activation by stabilizing IκB-α, thereby reducing nuclear translocation of NF-κB and downstream cytokine production in response to stress or injury. This suppression attenuates pro-inflammatory gene expression, such as Bcl-xL, thereby fine-tuning immune responses in various tissues.^[63]^[64]^[65]^[66] Recent studies highlight clusterin's involvement in dynamic assemblies, where stress induces shifts from soluble monomeric forms to fibril-bound complexes, altering its chaperone activity in dementia-related pathologies. Under oxidative or proteotoxic stress, clusterin transitions to bind amyloid fibrils, such as those of Aβ or tau, forming stable aggregates that modulate fibril propagation and toxicity. A 2023 study demonstrated decreased plasma clusterin levels in demented individuals, correlating with disrupted clusterin-fibril dynamics and enhanced fibrillar burden in aging brains. These stress-responsive assemblies, observed in 2023-2025 investigations, suggest clusterin adapts conformationally to buffer protein misfolding but may exacerbate seeding if dysregulated.^[67]^[68]^[52]

Clinical and Pathological Roles

Neurodegenerative Diseases

Clusterin (CLU), particularly its secreted isoform sCLU, plays a significant role in Alzheimer's disease (AD) pathogenesis through its involvement in amyloid-β (Aβ) clearance and proteostasis. The single nucleotide polymorphism rs11136000 in the CLU gene, where the C allele increases AD risk with an odds ratio of approximately 1.2, has been consistently associated with late-onset AD across large-scale genome-wide association studies.^[11] This variant influences CLU expression levels, with the risk allele linked to altered chaperone function that impairs Aβ handling. Elevated levels of clusterin in cerebrospinal fluid (CSF) and plasma are observed in AD patients and correlate with faster rates of brain atrophy and cognitive decline, as evidenced by longitudinal cohorts showing higher plasma clusterin predicting progression from mild cognitive impairment to dementia.^[69]^[70] In AD, chaperone failure contributes to reduced Aβ clearance, leading to plaque accumulation; sCLU binds to Aβ fibrils and oligomers to prevent aggregation, but its efficacy diminishes in aging and dementia. sCLU binds Aβ with high affinity to suppress aggregation and fibril formation. This decline is particularly notable in plasma, where a 2023 study reported decreased clusterin levels in demented elderly individuals compared to controls, with a steeper age-related drop (approximately 20% lower in demented groups), disrupting the balance between clusterin and fibrillar structures.^[71] In Parkinson's disease, clusterin exerts a protective effect by binding α-synuclein aggregates, inhibiting their propagation and uptake by glial cells, thereby mitigating synucleinopathy progression.^[52] Therapeutic strategies targeting clusterin have shown promise in preclinical models. A 2025 discovery identified small molecule enhancers, such as the brain-permeable BET inhibitor DDL-357, which boost sCLU secretion and reduce AD pathology; in 3xTg-AD mice, 6-week dosing decreased phospho-tau levels and improved memory performance in behavioral assays.^[62] These findings underscore clusterin's potential as a biomarker and therapeutic target in neurodegenerative diseases, with ongoing research focusing on modulating its levels to enhance clearance mechanisms.

Oncological Implications

Clusterin exhibits context-dependent roles in oncogenesis, frequently acting as a pro-tumor factor through its overexpression in various malignancies. It is upregulated in prostate, breast, and ovarian cancers, where elevated levels correlate with advanced disease stages and poorer clinical outcomes.^[72] This overexpression promotes tumor cell survival and progression by enhancing resistance to chemotherapy, particularly via activation of the Akt signaling pathway, which inhibits apoptosis and supports cytoprotection against agents like docetaxel and cisplatin.^[73] For instance, secreted clusterin (sCLU) stabilizes survival pathways in prostate and lung cancer cells, contributing to therapeutic resistance.^[74] Despite its predominantly pro-oncogenic effects, clusterin demonstrates anti-tumor potential in certain contexts, such as inhibiting metastasis in experimental models of lung and renal cell carcinoma. Cytoplasmic clusterin suppresses migration and invasion by modulating epithelial-mesenchymal transition and extracellular matrix interactions.^[75] Therapeutic targeting of clusterin has been pursued, notably with custirsen (OGX-011), an antisense oligonucleotide that inhibits sCLU expression; however, the phase III SYNERGY trial in 2014 showed no significant overall survival benefit when added to docetaxel and prednisone in metastatic castration-resistant prostate cancer.^[76] Ongoing preclinical efforts explore novel inhibitors, including advanced siRNAs and small molecule modulators, which sensitize cancer cells to treatment by disrupting clusterin-mediated survival signals.^[77] Glycosylation variants of clusterin further influence its oncogenic behavior, with hypersialylated forms prevalent in tumor microenvironments that enhance cell invasion and motility. These modified isoforms facilitate immune evasion and adhesion changes, underscoring glycosylation as a key regulator of clusterin's pro-invasive functions.^[78] As a biomarker, elevated serum clusterin levels predict poor prognosis in several cancers. This prognostic value stems from its role in sustaining tumor growth and resistance, though cytoplasmic forms may confer protective effects in some cases.

Cardiovascular and Renal Disorders

Clusterin (CLU), also known as apolipoprotein J, plays a protective role in cardiovascular disorders, particularly atherosclerosis, where it associates with high-density lipoprotein (HDL) to mitigate foam cell formation. In atherosclerotic lesions, HDL-bound CLU binds to aggregated low-density lipoprotein (LDL), preventing its precipitation and uptake by macrophages, thereby reducing the transformation into foam cells that drive plaque development.^[79] This chaperone activity extends to oxidized LDL, as CLU inhibits the aggregation of mildly oxidized LDL particles, which are key initiators of endothelial inflammation and atherogenesis.^[80] Elevated CLU levels in plasma and atherosclerotic walls reflect a compensatory response to oxidative stress, with immunolocalization increasing alongside HDL components like apolipoprotein A-I.^[81] In myocardial infarction (MI), CLU levels rise acutely post-reperfusion, originating partly from cardiac tissue, and serve as a prognostic biomarker for adverse outcomes such as left ventricular remodeling and reduced survival.^[82] Patients with ST-segment elevation MI and lower serum CLU exhibit higher risks of complications, including elevated Killip class and anterior infarction location, highlighting its association with worse cardiac function.^[83] Experimental models confirm CLU's cardioprotective effects, as CLU-deficient mice show increased susceptibility to ischemic injury, heightened inflammation, and elevated histone levels post-MI.^[84] Regarding renal disorders, CLU is upregulated in acute kidney injury (AKI), particularly in tubular cells, where it acts as an anti-apoptotic factor and potential early biomarker detectable in urine before serum creatinine rises.^[85] Urinary CLU predicts AKI onset following procedures like transcatheter aortic valve implantation, outperforming some traditional markers in subclinical cases, though 2023 reviews note its promise remains unvalidated for routine clinical use due to limited large-scale trials.^[86] In chronic kidney disease (CKD), CLU exerts anti-fibrotic effects by modulating transforming growth factor-β (TGF-β) signaling, inhibiting Smad3 phosphorylation and downstream extracellular matrix deposition in renal tubular epithelial cells.^[87] CLU deficiency exacerbates inflammation and fibrosis post-ischemia-reperfusion injury, underscoring its protective role against progressive renal scarring.^[88] Recent studies link reduced CLU expression to endothelial damage in hypertension, where low levels correlate with impaired vascular integrity and increased susceptibility to stress-induced dysfunction, amplifying hypertensive cardiovascular risk.^[89]

Infectious and Inflammatory Diseases

Clusterin plays a dual role in infectious diseases, acting as a complement regulatory protein that limits excessive immune-mediated damage to host tissues while sometimes facilitating viral persistence by shielding pathogens from lysis. In models of HIV infection, clusterin incorporates into the viral envelope during budding from host cells, where it inhibits complement activation and prevents antibody-dependent complement-mediated lysis of the virus.^[90] Similarly, in influenza A virus infection, clusterin contributes to host cell survival by modulating stress responses and reducing cytopathic effects, including protection against complement-driven lysis in infected lung tissues.^[91] For hepatitis C virus (HCV), clusterin's lipid-binding properties may indirectly influence viral entry by interacting with lipoprotein complexes that HCV exploits for attachment and uptake into hepatocytes, though direct inhibition remains under investigation. In inflammatory diseases, clusterin functions primarily as a regulator of the complement system, binding to fluid-phase complement components to inhibit membrane attack complex formation and thereby prevent bystander tissue damage during acute and chronic inflammation.^[92] This protective mechanism is evident in conditions like rheumatoid arthritis (RA), where clusterin levels are upregulated in synovial fluid and serum of patients, particularly in early disease stages, correlating with reduced joint destruction through complement modulation.^[93] Elevated clusterin in RA synovium, expressed by synoviocytes and infiltrating cells, also dampens TNF-α-driven inflammatory signaling, highlighting its role in local immune homeostasis.^[94] Mechanistically, the secreted isoform of clusterin (sCLU) exerts anti-inflammatory effects by neutralizing extracellular histones released during sepsis, which otherwise promote cytokine storms, thrombosis, and cytotoxicity in endothelial and immune cells.^[95] In contrast, the nuclear isoform (nCLU) can amplify chronic inflammation by interacting with stress signaling pathways, leading to enhanced cytokine release such as TNF-α from macrophages and promoting chemotactic responses that sustain inflammatory infiltrates.^[96] These isoform-specific actions underscore clusterin's context-dependent regulation of immune responses in inflammatory settings. Recent research has begun to address gaps in understanding clusterin's involvement in post-viral complications, particularly its interactions with amyloid fibrils in driving neurodegeneration following infections like influenza or coronaviruses. A 2023 study highlighted clusterin's binding to brain endothelial receptors to mitigate neuroinflammatory cascades triggered by viral-induced complement activation, potentially influencing fibril dynamics in post-viral states.^[97] A 2023 investigation into cerebrospinal fluid clusterin levels across the Alzheimer's disease continuum revealed dynamic changes influenced by AD pathologies, suggesting a role in modulating protein aggregation.^[98]

References

[1]
Structural analyses define the molecular basis of clusterin ... - Nature
Aug 8, 2025 · Here, we present crystal structures of human clusterin, revealing a discontinuous three-domain architecture. Structure-based mutational analysis ...
[2]
Clusterin: structure, function and roles in disease - PubMed Central
Jan 21, 2025 · The common function of cCLU protein is to inhibit cell apoptosis. ... Brain clusterin protein isoforms and mitochondrial localization.
[3]
Clusterin - an overview | ScienceDirect Topics
Clusterin (also known as apoJ) is a multifunctional heterodimeric protein that acts as a natural chaperone. In AD brains, apoJ is co-deposited with fibrillar Aβ ...
[4]
Clusterin in Alzheimer's Disease: Mechanisms, Genetics ... - Frontiers
Feb 27, 2019 · Clusterin (CLU) or APOJ is a multifunctional glycoprotein that has been implicated in several physiological and pathological states, including Alzheimer's ...Abstract · Clusterin Complexity: From... · Clusterin (dys)Function in AD · Conclusion
[5]
The multiple roles and therapeutic potential of clusterin in non-small ...
In this review, we will firstly discuss the structure and physiology function of CLU, then about the role of CLU in tumorigenesis, metastasis, chemotherapy ...Introduction · Structure and biological... · CLU in lung cancer · Conclusions
[6]
1191 - Gene ResultCLU clusterin [ (human)] - NCBI
The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol.Missing: cytogenetic | Show results with:cytogenetic
[7]
Entry - *185430 - CLUSTERIN; CLU - OMIM - (OMIM.ORG)
(1991) concluded that a single gene is responsible for the multiple functions of sulfated glycoprotein-2 and that the SGP2 gene is located on human chromosome ...
[8]
https://www.ncbi.nlm.nih.gov/nuccore/NM_001831.4
[9]
Non-Secreted Clusterin Isoforms Are Translated in Rare Amounts ...
The human CLU gene encodes at least 3 different pre-mRNAs which contain unique exons 1 but share exons 2-9. Alternative splicing of variant 1 pre-mRNA generates ...
[10]
Common Alzheimer's Disease Risk Variant Within the CLU Gene ...
May 4, 2011 · A newly confirmed genetic risk allele C of the clusterin (CLU) gene variant rs11136000 is carried by ∼88% of Caucasians. The C allele ...
[11]
Genetic variant rs11136000 upregulates clusterin expression and ...
rs11136000 may reduce AD risk by regulating CLU expression, which may provide important information about the biological mechanism of rs9848497 in AD progress.
[12]
Hypoxia Inducible Factor-1α Directly Regulates Nuclear Clusterin ...
Here, we examined the transcriptional regulation of nCLU in response to hypoxia. We identified three putative hypoxia response elements (HREs) in the human CLU ...
[13]
Contribution of the Transcription Factors Sp1/Sp3 and AP-1 to ...
We demonstrated that CLU gene transcription was indeed ensured in part by the binding of the TFs Sp1/Sp3 and AP-1 to overlapping target sites within the basal ...
[14]
https://www.mdpi.com/1422-0067/22/22/12426
[15]
The Ins and Outs of Clusterin: Its Role in Cancer, Eye Diseases and ...
Studies have revealed that sCLU functions as an extracellular chaperone that binds to hydrophobic regions of partially unfolded proteins and inhibits, via an ...<|control11|><|separator|>
[16]
CLU - Clusterin - Homo sapiens (Human) | UniProtKB | UniProt
Isoform 4 has been previously detected in cytosol and in the nuclei of apoptotic cells and promoted apoptosis following irradiation (PubMed:12551933). However ...Missing: cytogenetic | Show results with:cytogenetic
[17]
The Influence of Clusterin Glycosylation Variability on Selected ...
The chains are linked by five disulfide bonds and form a two-chain, antiparallel glycoprotein structure. The core of the clusterin molecule is surrounded by ...
[18]
Clusterin, a binding protein with a molten globule-like region - PubMed
Consistent with this last idea, clusterin has been shown to bind to a variety of molecules with high affinity including lipids, peptides, and proteins and the ...
[19]
CRM1 Protein-mediated Regulation of Nuclear Clusterin (nCLU), an ...
In contrast, sCLU levels (generated from expression from full-length CLU mRNA) were not significantly altered (p > 0.5) by siRNA-nCLU or siRNA-Scr transfection.Missing: size | Show results with:size
[20]
Proapoptotic role of nuclear clusterin in brain - PMC - PubMed Central
The product is detected as the ~49 kDa nonglycosylated precursor nCLU in the cytosol and as a ~55 kDa glycosylated protein (nCLU) in the nucleus [8]. The ...
[21]
CLU (clusterin)
Oct 1, 2009 · Clusterin gene is 17876 bp long and contains 10 exons in total. First two exons are alternative (designated 1 and 1) used by two different transcript isoforms.
[22]
Serum Clusterin Concentration and Its Glycosylation Changes as ...
Apr 10, 2024 · CLU contains six N-glycosylation sites: Asn-86, Asn-103, Asn-145, Asn-291, Asn-354, and Asn-374. Seven types of CLU N-glycans are known, which ...
[23]
The Chaperone Activity of Clusterin is Dependent on Glycosylation ...
Nov 3, 2014 · During its maturation, the sCLU-precursor is N-glycosylated and cleaved into an α- and a β-chain, which are connected by five symmetrical ...Material And Methods · Chaperone Activity Assay · Results
[24]
CLU protein expression summary
The RNA data was used to cluster genes according to their expression across tissues. Clusters contain genes that have similar expression patterns, and each ...
[25]
Clusterin: Always protecting. Synthesis, function and potential issues
From the initial protein precursor, different isoforms can generate: a small nuclear isoform (∼49 kDa)a medium size that remains in the cytosol and mitochondria ...
[26]
Contribution of the Transcription Factors Sp1/Sp3 and AP-1 to ...
Nov 17, 2021 · Contribution of the transcription factors Sp1/Sp3 and AP-1 to clusterin gene expression during corneal wound healing of tissue-engineered human corneas.
[27]
The Molecular Chaperone Apolipoprotein J/Clusterin as a Sensor of ...
May 15, 2013 · Moreover, CLU gene expression was found to be regulated by NF-κB ... Regulation of CLU gene expression by oncogenes and epigenetic factors ...
[28]
Nuclear clusterin accumulation during heat shock response
Dec 5, 2005 · In heat shock response, CLU is considered a stress-inducible, pro-survival/cyto-protective factor via an HSE element present in his promoter.
[29]
Clusterin is a Gene Specific Target of MicroRNA-21 in Head ... - NIH
miRNA-21 was upregulated in HNSCC and stimulated cell growth. Integrated analyses identified Clusterin (CLU) as a potential miRNA-21 gene target.
[30]
Alternative Splicing Regulation of an Alzheimer's Risk Variant in CLU
Sep 25, 2020 · We investigated the effect of an AD risk variant (rs7982) in the 5th exon of CLU on alternative splicing by using an integrative approach of brain-tissue-based ...
[31]
Clusterin Deficiency Exacerbates Hyperoxia-Induced Acute Lung ...
Accordingly, CLU has been reported to be overexpressed following increased oxidative stress in several cell lines, including airway epithelial cells [10], ...Missing: hypoxia | Show results with:hypoxia
[32]
The clusterin connectome: Emerging players in chondrocyte biology ...
Hypoxia upregulated 1 (HYOU1), a marker of protein misfolding under cellular stress ... IL-6 increases clusterin expression. Clusterin has been shown to be ...
[33]
Clusterin negatively modulates mechanical stress-mediated ... - Nature
Sep 21, 2022 · These findings imply that CLU is induced by mechanical stress and TGF-β1 and inhibits LF fibrotic responses via negative feedback regulation of ...
[34]
Therapeutic Potential of Clusterin Inhibition in Human Cancer - MDPI
... isoform of 49 kDa (pnCLU). In response to stress, the isoform undergoes a transformation into a functional nuclear variant known as nCLU, weighing 55 kDa.
[35]
Clusterin: a key player in cancer chemoresistance and its inhibition
Mar 20, 2014 · Structure of CLU gene and transcription products. (A) CLU gene maps on chromosome 8. The position of exons and introns on the gene is shown.
[36]
The Dual Roles of Clusterin in Extracellular and Intracellular ...
CLU as a unique chaperone performing critical and synergic roles in both intracellular and extracellular proteostasis.Missing: function | Show results with:function
[37]
Apolipoprotein J (clusterin) induces cholesterol export from ... - NIH
In this study a possible role for apo J in lipid transport was explored. Mouse peritoneal macrophages were incubated with acetylated low-density lipoprotein ( ...
[38]
The Impact of Cholesterol, DHA, and Sphingolipids on Alzheimer's ...
On one hand, clusterin serves as a major lipid binding protein [35]. Interestingly, clustrin mRNA and protein levels are increased in AD, thereby ...
[39]
Apolipoprotein J (Clusterin) and Alzheimer's Disease
Brain perfusion studies in guinea pigs have documented high affinity transport for apoJ at the blood-brain barrier and choroid epithelium in vivo with Km of 0.2 ...
[40]
Clusterin and Its Role in Insulin Resistance and the Cardiometabolic ...
Feb 24, 2021 · Clusterin is found in a subset of dense HDL cholesterol particles and has wide-ranging effects on lipid transport (121, 128). In plasma, ...Missing: adhesion | Show results with:adhesion
[41]
Clusterin in the male reproductive system: localization and possible ...
May 25, 1999 · Clusterin is a glycoprotein that was initially isolated from the male reproductive system. Subsequently, clusterin has been found to be widely ...
[42]
Clusterin expression and distribution in spermatozoa as predictor of ...
Clusterin (CLU), one of the main glycoproteins in mammalian semen and the male reproductive tract, plays a role in spermatogenesis and sperm maturation. Given ...
[43]
Multiple involvement of clusterin in chicken ovarian follicle ... - PubMed
Feb 12, 1999 · In the ovary, clusterin is a major product of the somatic granulosa cells, in a pattern correlating with the developmental phases of individual ...
[44]
roles for clusterin in pathogenesis, progression and therapy of cancer
Sep 23, 2005 · Full-length CLU mRNA and sCLU protein were both involved in intestinal tumours. In prostate cancer, sCLU expression was associated with ...
[45]
Intracellular Clusterin Inhibits Mitochondrial Apoptosis by ...
We discovered that increased apoptosis in sCLU-depleted cells correlates to altered ratios of proapoptotic to antiapoptotic Bcl-2 protein family members, is ...
[46]
https://www.jbc.org/article/S0021-9258(20)59736-6/fulltext
[47]
Clusterin facilitates COMMD1 and I-κB degradation to enhance NF ...
We report that sCLU increases NF-κB nuclear translocation and transcriptional activity by serving as a ubiquitin binding protein that enhances COMMD1 and I-κB ...
[48]
Human nuclear clusterin mediates apoptosis by interacting with Bcl ...
May 12, 2011 · The product is detected as a ∼49 kDa non-glycosylated precursor clusterin (pnCLU) in the cytosol and a ∼55 kDa glycosylated protein (nuclear ...
[49]
Clusterin Binds to Aβ1–42 Oligomers with High Affinity and ...
We found that preincubation with clusterin antagonizes the toxic effects of Aβ 1–42 oligomers, as evaluated in a recently developed in vivo model in ...Analysis Of Aβ Oligomers By... · Clusterin Inhibits Aβ... · Clusterin Inhibits The...Missing: partners tau synuclein Kd
[50]
Clusterin Binding Modulates the Aggregation and Neurotoxicity of ...
Jul 20, 2022 · Synthetic CLU spontaneously bound to different possible variants of Aβ42 aggregates with very high affinity (Kd = 2.647 nM) in vitro to form ...
[51]
The extracellular chaperone Clusterin enhances Tau aggregate ...
Aug 11, 2021 · Our results show that Clu can strongly enhance Tau aggregate propagation by binding and stabilizing seeding active Tau species for cellular uptake.
[52]
The chaperone Clusterin in neurodegeneration−friend or foe?
May 6, 2022 · Clusterin is an abundant extracellular chaperone with a complex role in neurodegenerative pathologies associated with protein aggregation ...
[53]
[PDF] Balancing cholesterol in the brain: from synthesis to disposal
Jan 5, 2022 · Cholesterol is trafficked in the brain in association with ApoE or clusterin in HDL-like particles. Astrocytes are the primary source of ...
[54]
Clusterin/ApoJ enhances central leptin signaling through Lrp2 ...
May 13, 2014 · In cultured neurons, clusterin enhances receptor binding and subsequent endocytosis of leptin. These effects are mainly mediated through the LDL ...Missing: proteostasis | Show results with:proteostasis
[55]
Clusterin, the human apolipoprotein and complement inhibitor ...
Aug 15, 1993 · Together with vitronectin, clusterin binds to the nascent amphiphilic C5b-9 complex, rendering it water soluble and lytically inactive. To ...
[56]
Potent Inhibition of Terminal Complement Assembly by Clusterin
... C5b-9 and also bound to C5b-7 to prevent membrane ... Crystal structure of the membrane attack complex assembly inhibitor BGA71 from the Lyme disease agent ...
[57]
https://journals.physiology.org/doi/full/10.1152/ajpregu.2000.278.3.R640
[58]
Clusterin, a Novel Modulator of TGF-beta Signaling, Is ... - PubMed
CLU is a novel modulator of TGF-beta signaling by regulating Smad2/3 proteins. Overexpression of CLU enhanced TGF-beta-induced transcriptional activity.Missing: feedback | Show results with:feedback
[59]
Clusterin induced by N,N'-Dinitrosopiperazine is involved in ...
Feb 2, 2016 · The various plasmids containing different CLU promoter, Clusterin–Luc plasmids, Clusterin ... CLU co-localizes and binds to VEGF. DNP-treated or ...
[60]
Low Clusterin Levels in High Density Lipoprotein Associate ... - NIH
Clusterin levels in HDL are lower in men with reduced insulin sensitivity, higher body mass index, and an unfavorable lipid profile.
[61]
Role of clusterin in the brain vascular clearance of amyloid-β | PNAS
Aug 1, 2017 · CLU facilitates Aβ clearance across the BBB via LRP2 and also inhibits Aβ oligomerization and aggregation. Aβ bound to apoE4 is very slowly ...Missing: sCLU | Show results with:sCLU
[62]
Discovery of a small molecule secreted clusterin enhancer that ...
May 2, 2025 · We present findings from our drug discovery program to identify small molecules that enhance sCLU levels and assess their impact on AD pathology and cognition.
[63]
Clusterin: structure, function and roles in disease
The human CLU gene is located on the short arm of chromosome 8, spanning from region 2 to region 1, covering approximately 16 kb and comprising 11 exons.Missing: cytogenetic | Show results with:cytogenetic
[64]
CRM1 Protein-mediated Regulation of Nuclear Clusterin (nCLU), an ...
nCLU-mediated apoptosis was Bax-dependent, and lethality correlated with accumulation of mature nCLU protein. nCLU accumulation was regulated by CRM1 because ...
[65]
Identification of Clusterin Domain Involved in NF-κB Pathway ... - NIH
It has been shown that ectopic CLU expression strongly inhibited NF-κB activity by stabilizing inhibitors of NF-κB (IκB) (16). The presence of clusterin reduced ...Results · Clu Molecular Constructs · Figure 2Missing: SP1 | Show results with:SP1<|control11|><|separator|>
[66]
Inhibition of NF-κB-dependent Bcl-xL expression by clusterin ...
Mar 1, 2008 · Effects of PTEC clusterin, IκBα, and NF-κB p65 on BSA-induced apoptosis. We focused on NF-κB as a central mediator of inflammation, IκBα as an ...
[67]
Monitoring clusterin and fibrillar structures in aging and dementia
This study reveals a significant decrease in plasma clusterin in demented individuals with aging, which related to altered clusterin-fibrils dynamics.Clu Genotyping · Clusterin Plasma Levels In... · Fig. 3Missing: 2024 2025<|control11|><|separator|>
[68]
Methionine oxidation of clusterin in Alzheimer's disease and its ...
Jul 27, 2024 · We suggest that methionine oxidation of brain-clusterin is enhanced in Alzheimer's disease and that this oxidation compromises its chaperon function.
[69]
Clusterin Levels in Plasma Predict Cognitive Decline and ...
Conclusion: Elevated plasma clusterin levels in MCI confer an increased risk for progression to AD, and more rapid cognitive decline. We speculate that ...
[70]
Plasma Clusterin and the Risk of Alzheimer Disease - JAMA Network
Apr 6, 2011 · Plasma clusterin was reported to be associated with brain atrophy, baseline disease severity, and rapid clinical progression in patients with AD.Missing: decline | Show results with:decline
[71]
The Emerging Role of the Molecular Chaperone Clusterin in ... - PMC
Jul 1, 2025 · Clusterin (CLU) is a heterodimeric, ATP-independent molecular chaperone that exhibits high expression in the brain.
[72]
Monitoring clusterin and fibrillar structures in aging and dementia
Clusterin assists in the folding of secreted proteins and functions as an extracellular chaperone to prevent the aggregation of non-native proteins, including ...
[73]
Clusterin expression in nontumor tissue in patients with resectable ...
It is upregulated in several types of cancers such as breast, ovarian, colon, prostate and kidney cancers, and HCC. Clusterin overexpression is correlated with ...<|control11|><|separator|>
[74]
Secreted clusterin gene silencing enhances chemosensitivity of ...
Secreted clusterin gene silencing enhances chemosensitivity of a549 cells to cisplatin through AKT and ERK1/2 pathways in vitro. Cell Physiol Biochem. 2014 ...
[75]
role in cytoprotection against docetaxel in prostate tumor cells
May 25, 2010 · Induction of clusterin by AKT--role in cytoprotection against docetaxel in prostate tumor cells ... Thus, the chemoresistance to docetaxel ...
[76]
Cytoplasmic Clusterin Suppresses Lung Cancer Metastasis by ...
May 17, 2022 · Impressively, overexpressing CLU potently inhibits the migration, invasion and metastasis of lung cancer cells, whereas silencing CLU promotes ...
[77]
Custirsen in combination with docetaxel and prednisone for patients ...
Mar 8, 2017 · The aim of the SYNERGY trial was to investigate the effect of custirsen in combination with docetaxel and prednisone on overall survival in patients with ...Missing: results | Show results with:results
[78]
A potential avenue for targeted cancer therapy - PubMed
Dec 29, 2020 · Clusterin (CLU) is an evolutionary conserved molecular chaperone present in different human tissues and fluids and established to be a significant cancer ...<|control11|><|separator|>
[79]
The Influence of Clusterin Glycosylation Variability on Selected ...
Aug 28, 2022 · The present review gathers together the most important information about variability in clusterin molecular structure, its profile, and the degree of ...
[80]
Prognostic Role of Secretory Clusterin in Multiple Human Malignant ...
... hazard ratio (HRs) or risk ratio ... Expression of the secreted form of clusterin protein in renal cell carcinoma as a predictor of disease extension.
[81]
Cytoplasmic Clusterin Expression Is Associated with Longer ...
Poor prognosis has been associated with clusterin staining in renal cell carcinoma ... Differential expression of serum clusterin isoforms in colorectal cancer.
[82]
Clusterin/apolipoprotein J binds to aggregated LDL in human ...
Dec 30, 2014 · These findings point to apoJ as a key modulator of LDL aggregation and reveal a putative new therapeutic strategy against atherosclerosis.
[83]
Mildly oxidized LDL induces an increased apolipoprotein ... - PubMed
Apr 15, 1997 · We have examined the effects of mildly oxidized LDL and atherosclerosis on the levels of two proteins associated with HDL; apolipoprotein J ...
[84]
Increased Immunolocalization of Paraoxonase, Clusterin, and ...
The increase in Pon, clusterin, and apoA-I during the development of atherosclerosis may therefore represent a protective response to the oxidative stress ...
[85]
Expression and Implication of Clusterin in Left Ventricular ...
Jun 11, 2018 · Our results show for the first time that plasma CLU levels are associated with LVR post–myocardial infarction, have in part a cardiac origin, and are a ...
[86]
Prognostic value of clusterin/apolipoprotein J in patients with ...
Oct 28, 2022 · Patients with serum CLU < median were more likely to be older, present with anterior myocardial infarction and in a higher Killip class on ...
[87]
Cardioprotective effects of the extracellular chaperone clusterin in ...
May 26, 2025 · Under cell stress and tissue injury, CLU is up-regulated and exerts anti-inflammatory and cytoprotective effects. Studies have reported ...
[88]
Advances in the study of subclinical AKI biomarkers - Frontiers
However, in a recent study, Clusterin outperformed renal function indicators targeting both glomeruli and tubules in the assessment of subclinical AKI, ...
[89]
Clinical usefulness of urinary biomarkers for early prediction of acute ...
Oct 30, 2023 · This study aimed to reveal the clinical usefulness of urinary biomarkers for the early prediction of AKI onset after transcatheter aortic valve implantation ( ...
[90]
Elucidating the function of clusterin in the progression of diabetic ...
Overexpression of Clu in RTECs has been shown to diminish the expression of TGFβ-induced fibrosis related proteins by inhibiting Smad3 phosphorylation and its ...The function of Clu in DKD · Functions of Clu in kidney... · Role of Clu in DKD
[91]
Clusterin Attenuates the Development of Renal Fibrosis - PMC
These results suggest that upregulation of clusterin during renal injury is a protective response against the development of renal fibrosis.
[92]
Clusterin ameliorates endothelial dysfunction in diabetes by ...
Clusterin exerts beneficial effects in endothelial cells under diabetic conditions via inhibiting mitochondrial fragmentation mediated by AMPK.
[93]
The good and evil of complement activation in HIV-1 infection - PMC
Complement regulators on the surface of HIV-1 protect HIV-1 from complement-mediated lysis. As we discussed extensively above, the complement system, a key ...
[94]
Mechanistic Insights into Influenza A Virus-Induced Cell Death and ...
... Protein Clusterin. ... Cellular Inhibitor of Apoptosis Protein Ciap2 Protects against Pulmonary Tissue Necrosis during Influenza Virus Infection to Promote Host ...
[95]
Complement Regulator of Complement System: Clusterin / CLU
The mature form of human secreted CLU is a secreted heterodimeric glycoprotein of 75-80 kDa. As other secreted proteins, translation of CLU mRNA results in a ...What Is Clusterin? · Clusterin Test · Clusterin In Health And...<|separator|>
[96]
Clusterin serum levels are elevated in patients with early rheumatoid ...
We found increased serum concentrations of clusterin in treatment-naïve patients with early rheumatoid arthritis, and we suggest clusterin as a predictive ...
[97]
https://www.nature.com/articles/s41392-023-01486-5
[98]
Clusterin Neutralizes the Inflammatory and Cytotoxic Properties of ...
Jul 4, 2022 · Clusterin Neutralizes the Inflammatory and Cytotoxic Properties of Extracellular Histones in Sepsis ... However, CLU and APC differ in the ...Missing: sCLU | Show results with:sCLU
[99]
Clusterin induces the secretion of TNF-α and the chemotactic ...
Aug 6, 2025 · [65] Studies have shown that clusterin stimulates TNFa release in macrophages and regulates the expression of chemotactic cytokines. [106, 107] ...
[100]
Role of neuroinflammation in neurodegeneration development
Jul 12, 2023 · Clusterin, one of the complement cascade inhibitors, can bind to the receptor on brain endothelial cells and reduce the expression of ...
[101]
(PDF) Dynamic changes of CSF clusterin levels across the ...
Aug 6, 2025 · This meta-analysis indicates that high CLU concentration in the plasma and brain is associated with dementia, especially in AD. View. Show ...