Fact-checked by Grok 2 weeks ago

Disassortative mating

Disassortative mating, also known as negative assortative mating or heterogamy, is a non-random mating pattern in biology where individuals preferentially select partners with dissimilar phenotypes or genotypes, in contrast to assortative mating where similar individuals pair more frequently.^[1] This preference promotes genetic diversity by increasing heterozygosity in offspring, reducing the risks associated with inbreeding and enhancing overall population variability.^[2] In evolutionary terms, disassortative mating plays a key role in maintaining genetic polymorphism and can evolve under conditions of heterozygote advantage or negative frequency-dependent selection, where rare alleles confer higher fitness.^[2] It often arises in contexts where genetic compatibility benefits offspring survival, such as improved immune responses through diverse alleles at loci like the major histocompatibility complex (MHC).^[3] For instance, in humans, studies of European populations have shown that couples exhibit greater MHC dissimilarity than expected by chance, potentially reducing offspring homozygosity by about 1% and bolstering pathogen resistance.^[3] Examples of disassortative mating span diverse taxa, illustrating its adaptive value. In vertebrates, female preferences for MHC-dissimilar males occur in mammals, birds, fish, and reptiles, supporting broader immune diversity in progeny.^[3] Among birds, the white-throated sparrow displays plumage-based disassortative mating that sustains morph polymorphism.^[2] In insects, Heliconius numata butterflies show strong disassortative preferences for wing pattern morphs, linked to heterozygote advantage at supergene loci.^[2] Plants enforce disassortative mating through structural adaptations like dioecy (separate sexes) or heterostyly (differing flower styles), which prevent self-pollination and foster hybrid vigor, as seen in increased yields from outcrossed corn varieties.^[1] Overall, while rarer than assortative mating, disassortative strategies contribute to long-term adaptability by countering genetic homogenization.^[2]

Fundamentals

Definition

Disassortative mating is a form of non-random mating in which individuals preferentially pair with others exhibiting dissimilar phenotypes or genotypes, occurring more frequently than would be predicted by random mating alone.^[2] This pattern contrasts with assortative mating, where similarity drives mate choice.^[4] The concept of disassortative mating was first formalized in population genetics by Sewall Wright in his 1921 series of papers on mating systems, where it was described as a mechanism involving pairing based on somatic dissimilarity, diverging from the random mating assumption central to the Hardy-Weinberg equilibrium model established in 1908. Wright's work highlighted how such mating deviates from panmixia, influencing genetic correlations between relatives.^[5] A key characteristic of disassortative mating is its tendency to increase heterozygosity in offspring by promoting unions between genetically diverse individuals, thereby reducing homozygosity compared to random expectations.^[6] It is commonly quantified using correlation coefficients, where negative values (e.g., mating correlation r < 0) indicate a preference for dissimilarity in traits or alleles.^[7] In a basic two-allele model at a single locus (with alleles A and a), disassortative mating manifests as an elevated frequency of pairings between dissimilar genotypes (e.g., AA \times aa, AA \times Aa, aa \times Aa) relative to random proportions, while similar pairings (e.g., AA \times AA, aa \times aa, Aa \times Aa) occur less often. This can be represented by the probability of dissimilar matings exceeding the product of genotype frequencies under equilibrium, such as P(AA \times aa) > p^2 q^2, where p and q are allele frequencies, thereby shifting genotypic proportions toward greater heterozygote production.^[8]

Comparison with Assortative Mating

Assortative mating refers to a non-random mating pattern characterized by a positive correlation (r > 0) between the phenotypes or genotypes of mating partners, where individuals preferentially pair with those exhibiting similar traits.^[9] This contrasts with disassortative mating, which involves a negative correlation (r < 0), favoring pairings between dissimilar individuals.^[9] Random mating serves as the neutral baseline for both patterns, where mate choice occurs without regard to traits (r = 0), producing offspring genotype frequencies expected under Hardy-Weinberg equilibrium.^[10] Key differences between the two mating patterns emerge in their genetic consequences. Disassortative mating reduces homozygosity by promoting unions between dissimilar genotypes, thereby decreasing overall trait variance within populations and promoting genetic mixing to reduce phenotypic divergence.^[11] In contrast, assortative mating increases homozygosity and trait variance, which can accelerate genetic differentiation and contribute to speciation by limiting gene flow between dissimilar groups.^[11]^[12] These effects are summarized in the following comparison:

Aspect	Assortative Mating (r > 0)	Disassortative Mating (r < 0)
Homozygosity	Increases	Decreases
Trait Variance	Increases (additive genetic variance rises)	Decreases
Population Divergence	Reinforces, potentially leading to speciation	Reduces, promoting genetic mixing
Gene Flow	Reduces between dissimilar phenotypes	Increases between dissimilar phenotypes

^[11]^[13] Evolutionarily, disassortative mating often arises under conditions of heterozygote advantage, where hybrid offspring gain fitness benefits from genetic complementarity, maintaining polymorphism.^[14] Assortative mating, however, typically evolves via disruptive selection, favoring extreme phenotypes and driving divergence between subpopulations.^[15] This disassortative maintenance of genetic diversity can buffer against inbreeding depression in polymorphic systems.^[2]

Types

Genetic Disassortative Mating

Genetic disassortative mating refers to the preference for mates carrying dissimilar alleles at specific genetic loci, which enhances heterozygosity in offspring and thereby improves their fitness. This phenomenon is prominently observed at the major histocompatibility complex (MHC) loci in vertebrates, where individuals select partners with differing MHC genotypes to optimize immune system diversity in progeny. The MHC encodes proteins crucial for immune recognition of pathogens, and mating with dissimilar MHC alleles allows offspring to present a broader range of antigens, conferring resistance to a wider array of diseases.^[16] Such preferences have been documented across species, including mice, fish, and birds, through controlled experiments demonstrating non-random pairing based on MHC genotypes.^[17] In plants, genetic disassortative mating manifests through self-incompatibility (SI) systems governed by S-loci, which enforce outcrossing by rejecting pollen from genetically identical or closely related individuals. These multi-allelic loci, such as the S-RNase and SLF/SFB genes in gametophytic SI or the SRK and SCR/SP11 genes in sporophytic SI, recognize self-pollen via specific interactions that halt fertilization. By preventing self-fertilization, S-loci maintain high allelic diversity and promote gene flow, a mechanism evolved independently in more than 100 angiosperm families to counteract inbreeding.^[18] Measurement of genetic disassortative mating often involves genotyping parental pairs and offspring to detect deviations from random expectations, such as an excess of heterozygous progeny at target loci. For MHC dissimilarity, a common index is calculated as d = 1 - \frac{2s}{n}, where s is the number of shared alleles between mates and n is the total number of distinct alleles across both individuals; higher d values indicate greater dissimilarity and support disassortative preferences when observed more frequently than under random mating.^[19] Genotyping studies in humans and other vertebrates have revealed such excesses, with couples showing significantly higher MHC heterozygosity in offspring compared to neutral loci.^[3] The evolutionary rationale for genetic disassortative mating centers on avoiding inbreeding depression—the reduced fitness from homozygous deleterious alleles—and fostering adaptive genetic variation. By favoring dissimilar mates, this strategy minimizes genome-wide homozygosity, particularly at immune-related loci like MHC, thereby enhancing offspring survival against parasites and environmental challenges. In plants, SI loci similarly sustain polymorphism under balancing selection, preventing the fixation of alleles that could lead to selfing and genetic erosion.^[20]

Phenotypic Disassortative Mating

Phenotypic disassortative mating occurs when individuals preferentially select mates exhibiting dissimilar observable traits, such as physical characteristics or behavioral tendencies, without reliance on genetic testing or assays. This form of mate choice contrasts with random mating by promoting pairings between phenotypically distinct individuals, often based on traits like body size, coloration, or activity levels that can be directly assessed through observation. Such preferences may enhance compatibility or reduce competition in polymorphic populations, though they remain relatively rare compared to assortative mating patterns.^[9] A prominent example is found in the butterfly Heliconius numata, where females exhibit a preference for males with wing color patterns that differ from their own, particularly in polymorphic populations displaying multiple mimetic morphs.^[2] This disassortative choice based on visual cues helps avoid producing poorly mimetic offspring that are more vulnerable to predators, thereby maintaining polymorphism through frequency-dependent selection. Similarly, in guppies (Poecilia reticulata), disassortative mating for boldness has been observed, though such pairings decrease reproductive success compared to assortative ones.^[21] This mating pattern is typically measured using observational data on pairing frequencies in natural or experimental settings, coupled with correlation analysis of phenotypic traits among mated pairs. A negative correlation coefficient (r < 0) between the trait values of partners signifies disassortative mating, quantifying the degree of phenotypic dissimilarity in successful pairings. These metrics help distinguish true preferences from random associations, often controlling for environmental factors in field studies.^[22]^[9] Unlike genetically driven disassortative mating tied to specific loci, phenotypic disassortative mating can emerge from environmental influences, such as learning or habitat variability, or from polygenic traits that do not involve single-gene effects. In some cases, underlying genetic factors may contribute to the expression of these observable traits, but the preferences are primarily assessed through external phenotypes.^[9]

Mechanisms

Molecular Mechanisms

Disassortative mating at the molecular level involves biochemical processes that enable organisms to detect and prefer genetically dissimilar partners, primarily through sensory and recognition systems. In mammals, olfactory cues play a central role, where peptides derived from the major histocompatibility complex (MHC) are detected, influencing mate choice to promote genetic diversity. These MHC peptides, bound to urinary proteins, are released in bodily scents and serve as signals of genetic compatibility.^[23] The detection of MHC peptides occurs via the vomeronasal organ (VNO) in mammals, a specialized chemosensory structure that processes pheromonal cues. Sensory neurons in the VNO express vomeronasal receptors (V2Rs) that bind MHC class I peptides with allele-specific affinity, triggering neural responses at low concentrations (as low as 10⁻¹² M). This mechanism allows individuals to distinguish MHC-dissimilar scents, leading to preferences for mates with complementary immune genes, thereby avoiding inbreeding and enhancing offspring immunocompetence. A seminal example is the "sweaty T-shirt" study, where women rated body odors from men wearing T-shirts for two nights; preferences favored odors from MHC-dissimilar men, particularly among women not using oral contraceptives, indicating hormonal modulation of this molecular pathway.^[23]^[24] In plants, molecular recognition of genetic dissimilarity occurs through protein interactions in pollen-pistil systems, notably the S-RNase-based self-incompatibility (SI) mechanism. This gametophytic system, prevalent in families like Solanaceae and Rosaceae, prevents fertilization by pollen sharing S-haplotypes with the pistil. S-RNases, secreted by pistil cells, are ribonucleases that enter pollen tubes; in cases of matching S-alleles (indicating genetic similarity), these enzymes degrade pollen RNA, arresting tube growth and rejecting self or related pollen. Non-matching (dissimilar) pollen neutralizes non-self S-RNases via S-locus F-box proteins, allowing fertilization and promoting outcrossing. This process ensures disassortative mating at the S-locus, maintaining allelic diversity.^[25] Gene expression within the MHC pathway further supports these preferences by regulating the production of detectable signals. MHC class I and II genes are upregulated in epithelial and glandular tissues, including those contributing to scent production, generating polymorphic peptides that encode individual odor profiles. In mammals, this expression influences volatile compounds in urine and sweat, which are perceived as dissimilar or similar based on allelic variation, driving assortative avoidance.^[26]^[27] Experimental evidence from knockout studies in mice confirms the necessity of MHC diversity for these preferences. Beta-2-microglobulin (β2m) knockout mice, which lack functional MHC class I expression due to impaired peptide presentation, produce indistinguishable urinary odors compared to wild-type controls, eliminating the chemical basis for MHC detection. Consequently, these mice show no disassortative mating preference, as the absence of MHC-specific volatile differences prevents recognition of genetic dissimilarity. Similar results in MHC congenic strains with homogenized haplotypes demonstrate reduced or absent odor-based mate choice, underscoring the direct role of MHC molecular integrity in enabling disassortative mechanisms.^[27]

Behavioral Mechanisms

Behavioral mechanisms of disassortative mating encompass the observable actions and strategies through which individuals express preferences for dissimilar mates, thereby implementing disassortative pairing in natural and experimental settings. These behaviors manifest during mate assessment and selection, often driven by sensory evaluation of phenotypic differences, and can be modulated by prior experiences and group interactions. In courtship displays, potential mates actively reject individuals exhibiting similar phenotypes, facilitating selection of dissimilar partners. For instance, in polymorphic butterflies like Heliconius numata, females dismiss males with matching wing patterns during close-range assessments, leading to preferential copulation with novel morphs that enhance mimicry diversity.^[28] This rejection behavior underscores how visual cues during courtship enforce disassortative preferences without requiring complex cognitive processing. Mate choice experiments further reveal active selection against similar traits in controlled environments. In guppies (Poecilia reticulata), laboratory trials demonstrate that females consistently favor males displaying rare or novel color morphs over those matching prevalent local variants, a pattern that sustains polymorphism and counters frequency-dependent predation. Such setups highlight the deliberate avoidance of phenotypic similarity, often quantified by higher association times with dissimilar options. Learning and imprinting contribute to disassortative mating by shaping avoidance of familiar traits, particularly through reverse imprinting where individuals eschew phenotypes resembling parental figures. Cross-fostering studies in house mice (Mus domesticus) show that early exposure to non-kin alters MHC-based preferences, resulting in behavioral rejection of mates similar to rearing environments and promoting outbreeding.^[29] This mechanism ensures disassortative choices even in the absence of direct kin recognition cues. Social contexts, including group dynamics, amplify disassortative pairing by influencing encounter rates and competition. In lekking systems and polymorphic bird populations, such as arctic skuas (Stercorarius parasiticus), individuals pair outside similar phenotypic clusters within display groups, where spatial segregation and competitive interactions favor dissimilar mates to maximize pairing success.^[30] These dynamics illustrate how communal settings reinforce behavioral preferences for diversity.

Evolutionary Effects

On Genetic Diversity

Disassortative mating promotes an increase in the frequency of heterozygotes within a population by favoring unions between genetically dissimilar individuals, thereby elevating observed heterozygosity above levels expected under random mating. This effect arises because such mating patterns preferentially produce heterozygous offspring from homozygous parents, counteracting the tendency toward homozygosity.^[31]^[32] This elevation in heterozygosity contributes to the maintenance of genetic polymorphism by reducing the probability of allele fixation due to genetic drift, particularly in small or finite populations. Models of multi-allelic systems demonstrate that disassortative mating can stabilize polymorphic equilibria, as the preference for dissimilar mates enhances the transmission of rare alleles and prevents their loss through random drift alone. For instance, in theoretical frameworks incorporating negative frequency-dependent selection via disassortative preferences, polymorphisms persist at intermediate frequencies, fostering long-term allelic diversity. Such dynamics are especially relevant at loci where heterozygote advantage interacts with mating preferences, leading to balanced equilibria that resist drift-induced erosion.^[33]^[34] Disassortative mating also serves to avoid inbreeding by minimizing matings between closely related or genetically similar individuals, thereby reducing the excess of homozygotes that would otherwise accumulate. This counters the Wahlund effect, where subpopulation structure might otherwise lead to heterozygote deficiencies at the total population level; by promoting outcrossing, disassortative patterns homogenize genotype frequencies and sustain higher heterozygosity across loci. In quantitative genetic terms, disassortative mating tends to reduce additive genetic variance compared to random mating, as it generates negative linkage disequilibrium that diminishes the heritable component of trait variation. This contrasts with positive assortative mating, which amplifies additive variance through positive LD, but the net effect under disassortative conditions is a more even distribution of genetic contributions to phenotypes, potentially stabilizing population-level responses to selection.^[35]^[36]

On Population Structure

Disassortative mating enhances gene flow between subpopulations by encouraging pairings among genetically dissimilar individuals, effectively mimicking increased migration rates and thereby reducing the fixation index (F_ST), a measure of genetic differentiation among populations. In heterostylous plants, for instance, this mating pattern, combined with negative frequency-dependent selection, promotes high levels of pollen-mediated gene dispersal, resulting in low fine-scale spatial genetic structure and minimal population differentiation. Such mixing homogenizes allele frequencies across demes, countering the isolating effects of geographic barriers. By favoring matings between divergent genotypes, disassortative mating resists speciation processes, particularly in hybrid zones where it sustains interpopulation admixture and prevents the evolution of strong reproductive isolation. Models indicate that complete disassortative mating equates to maximal gene flow (reproductive isolation index of 0), allowing hybrid zones to persist and even modest levels of such mating to halt divergence under strong selection. In systems with heterozygote advantage, disassortative mating further bolsters hybrid vigor, as seen in simulations where it maintains polymorphic loci and elevates hybrid fitness relative to parental forms. Disassortative mating contributes to demographic stability by elevating the effective population size (N_e) compared to assortative mating, as it minimizes inbreeding and variance in reproductive success, thereby preserving long-term population viability. In tristylous species with strong disassortative preferences, genetic estimates of N_e closely align with census sizes due to reduced selfing and enhanced outcrossing, contrasting with lower N_e in systems prone to assortative pairing. This increase in N_e supports greater resilience against stochastic fluctuations in population dynamics. Long-term simulations demonstrate that disassortative mating in structured populations, such as those modeled with migration, delays local adaptation by sustaining genetic variation and impeding the fixation of locally adaptive alleles. Numerical models reveal that negative frequency-dependent selection arising from disassortative preferences limits the spread of mating biases, preserving polymorphism and slowing divergence even under moderate gene flow. These dynamics briefly reference elevated allelic diversity at the population level, underscoring the broader connectivity fostered by such mating.

In Humans

Evidence from Studies

Empirical evidence for disassortative mating in humans has primarily focused on the major histocompatibility complex (MHC), a genomic region linked to immune function and odor profiles. One seminal study involved olfactory preference tests where women rated the attractiveness of body odors from T-shirts worn by men, finding a preference for scents from individuals with dissimilar MHC genotypes compared to their own.^[24] This "sweaty T-shirt" experiment, conducted with 49 Swiss participants, demonstrated that such preferences were stronger during the fertile phase of the menstrual cycle.^[24] Subsequent replications have confirmed similar patterns of odor-based MHC dissimilarity preferences, though effect sizes varied and some studies reported null results under specific conditions like oral contraceptive use. Genomic analyses of coupled individuals provide direct evidence of MHC disassortative mating. A 2019 study examined high-density genotype data from 883 European and Middle Eastern couples, revealing significant MHC disassortativity in Dutch (n=302 couples, p=0.040) and Northern European cohorts (n=658 couples from Belgium, Germany, Ireland, Netherlands, and UK; p=0.003), indicating non-random pairing for MHC dissimilarity beyond genome-wide patterns.^[3] In contrast, Israeli couples (n=70) showed no MHC disassortativity, with greater overall genomic similarity due to cultural endogamy.^[3] These findings suggest MHC-driven mate choice operates in outbred populations but is modulated by social factors. Population-level surveys of married or partnered couples yield mixed results on MHC similarity, with stronger disassortative signals in diverse groups. A 2017 meta-analysis of 12 human studies (n>1,000 couples) found a weak but significant preference for MHC-diverse mates (Zr=0.153, equivalent to r≈0.15), though no consistent disassortativity for specific alleles; effects were confounded by ethnic assortative mating in observational data.^[37] A 2020 meta-analysis of genomic and self-reported data across multiple cohorts confirmed weak overall effects (r≈-0.1 to -0.2 for similarity correlations in experimental subsets), emphasizing context-dependency in outbred versus endogamous societies.^[38] Cross-cultural variations highlight this, as endogamous Israeli Jewish groups exhibit reduced MHC disassortativity compared to European samples, where cultural openness correlates with higher heterozygosity in pairs.^[3] Disassortative mating in humans, particularly at the major histocompatibility complex (MHC) loci, can confer immune benefits to offspring by promoting greater MHC heterozygosity, which enhances resistance to a wider array of pathogens. This genetic diversity allows the immune system to recognize and combat more infectious agents effectively, as heterozygous individuals express a broader range of MHC molecules for antigen presentation.^[39] Studies indicate that such MHC dissimilarity between parents optimizes offspring immunocompetence, potentially reducing susceptibility to infectious diseases compared to more homozygous pairings.^[40] Regarding autoimmune diseases, the relationship is nuanced; while increased MHC diversity may elevate risks for some autoimmune conditions due to overactive immune responses, it has been linked in certain contexts to balanced protection that mitigates severe infectious outcomes without uniformly increasing autoimmunity.^[41] Evidence from studies suggests that pairs with MHC-based disassortativity have shown associations with improved offspring viability and parental fecundity, contributing to more successful reproduction over time.^[3] This pattern aligns with broader findings where genetic heterozygosity correlates with enhanced reproductive outcomes, though the effects vary by population and environmental factors.^[41] Socially, disassortative mating encourages exogamy, or marriage outside one's immediate kin or social group, which historically and contemporarily reduces the incidence of consanguineous unions and associated genetic risks. This practice fosters broader social networks and cultural exchange, potentially strengthening community resilience. However, implications for relationship stability reveal that couples with greater dissimilarity—whether in health, personality, or socioeconomic traits—often face higher divorce rates compared to more similar pairs, as mismatches can lead to compatibility challenges over time.^[42] In modern contexts, disassortative mating principles have influenced technology, such as dating applications that incorporate genetic matching to avoid deleterious recessive pairings, aiming to enhance offspring health. These tools, exemplified by initiatives from geneticists like George Church, spark ethical debates over eugenics, with critics arguing they commodify genetics and risk reinforcing social inequalities, while proponents emphasize voluntary disease prevention.^[43]

In Non-Human Species

Examples in Animals

In guppies (Poecilia reticulata), a species of freshwater fish, disassortative pairings based on boldness traits occur, where bold and shy individuals pair with those of opposite behavioral types. Laboratory experiments showed that such disassortative pairings resulted in reduced reproductive success, with pairs of dissimilar boldness producing fewer offspring compared to similar pairs, suggesting potential costs to this mating strategy.^[21] Among birds, the white-throated sparrow (Zonotrichia albicollis) provides a clear example of disassortative mating driven by visual traits, specifically crown plumage morphs: white-striped males almost exclusively pair with tan-striped females, and vice versa. This strict negative assortative mating maintains a balanced polymorphism in the population, with nearly 95% of pairs consisting of dissimilar morphs observed in wild populations across North America. The pattern is linked to a chromosomal inversion on the ZAL2 chromosome, which suppresses recombination and reinforces morph-specific behaviors, including territoriality and parental care differences that favor hybrid vigor in offspring. Field observations confirm that this disassortative choice occurs despite opportunities for assortative pairing, contributing to genetic diversity. In mammals, laboratory studies on house mice (Mus musculus domesticus) have validated disassortative mating preferences based on major histocompatibility complex (MHC) genes, where individuals avoid mates sharing similar MHC haplotypes to enhance offspring immune diversity. In controlled captivity trials, females reared with foster families of dissimilar MHC types reversed their natural preferences, actively avoiding males matching their foster family's MHC profile during mate choice assays, as evidenced by reduced mounting behaviors and confirmed by progeny genotyping showing higher MHC dissimilarity in actual pairings. These experiments highlight the role of olfactory cues and familial imprinting in driving MHC-based disassortative mating.^[44] Wild populations of grey mouse lemurs (Microcebus murinus), a solitary nocturnal primate in Madagascar, demonstrate disassortative mating at MHC class II DRB loci through field observations over a decade. Genetic analysis of mated pairs revealed significantly higher MHC DRB dissimilarity than expected under random mating, even after accounting for spatial proximity and kinship. This preference persists independently of inbreeding avoidance, as disassortative patterns held for unrelated dyads, and is likely mediated by scent signals during brief nocturnal encounters, promoting heterozygote advantage against pathogens in this high-density population.^[45] For invertebrates, the passion-vine butterfly Heliconius numata exhibits disassortative female choice based on polymorphic wing color patterns, which serve as warning signals in a Müllerian mimicry complex. In field and aviary trials in French Guiana, females preferentially courted males of dissimilar morphs, resulting in lower than expected assortative matings for color type. This behavior maintains adaptive polymorphism by increasing allelic diversity, as confirmed by genetic assays showing elevated heterozygosity in progeny from disassortative pairs, and underscores the role of visual cues in preventing morph fixation despite natural selection for mimicry conformity.^[46]

Examples in Plants

In plants, disassortative mating is primarily facilitated through self-incompatibility (SI) systems, which genetically prevent self-fertilization and related matings between individuals sharing similar alleles at the S-locus, thereby promoting outcrossing and genetic diversity.^[47] These systems are widespread, occurring in approximately half of all angiosperm species, underscoring their evolutionary persistence as a mechanism to avoid inbreeding depression.^[48] SI manifests in two main forms: gametophytic and sporophytic. In gametophytic self-incompatibility (GSI), the incompatibility response is determined by the haploid genotype of the pollen grain itself. For instance, in the Solanaceae family, such as species of Nicotiana and Petunia, pollen carrying an S-haplotype matching that of the style is rejected, ensuring only genetically dissimilar pollen successfully fertilizes the ovule.^[49] Conversely, sporophytic self-incompatibility (SSI) is controlled by the diploid genotype of the pollen parent, where dominance interactions among S-alleles influence pollen behavior. A prominent example is found in the Brassicaceae family, including Brassica species like cabbage and mustard, where self-pollen is recognized and inhibited on the stigma surface.^[50] At the molecular level, these systems rely on S-locus-encoded proteins that mediate recognition and rejection. In GSI of Solanaceae, S-RNases—ribonuclease glycoproteins secreted by the style—enter compatible pollen tubes but specifically degrade RNA in those matching the pistil's S-haplotype, halting tube growth and preventing fertilization.^[49] In SSI of Brassica, the stigma expresses S-locus glycoproteins (SLG) and S-receptor kinases (SRK), which interact with pollen-expressed S-cysteine-rich proteins (SCR/SP11); matching interactions trigger a signaling cascade that blocks pollen hydration and germination.^[51] These mechanisms enforce disassortative mating by rejecting pollen from plants with similar S-genotypes, thus favoring cross-pollination from diverse sources.^[52] Field studies illustrate the practical outcomes of these systems. In orchids (Orchidaceae), such as species in the genus Restrepia, SI leads to significantly higher seed set and fruit production following cross-pollinations with dissimilar S-haplotypes compared to self- or related pollinations, enhancing reproductive success in natural populations.^[53] Similarly, in mustards like Brassica rapa, controlled pollinations demonstrate that incompatible selfing results in near-zero seed set, while compatible outcrossing yields high seed viability, highlighting SI's role in promoting genetic mixing.^[54]

References

[1]
Outbreeding - an overview | ScienceDirect Topics
Outbreeding is also called genetic disassortative mating that is the reverse case of genetic assortative mating or inbreeding. Outbreeding is one of the five ...
[2]
When Do Opposites Attract? A Model Uncovering the Evolution of ...
Disassortative mating is a rare form of mate preference that promotes the persistence of polymorphism. While the evolution of assortative mating and its ...
[3]
Genomic evidence for MHC disassortative mating in humans - PMC
Mar 20, 2019 · Our findings support the hypothesis that the MHC influences mate choice in humans in a context-dependent way: MHC-driven preferences may exist ...
[4]
SYSTEMS OF MATING. III. ASSORTATIVE MATING BASED ON ...
Sewall Wright; SYSTEMS OF MATING. III. ASSORTATIVE MATING BASED ON SOMATIC RESEMBLANCE, Genetics, Volume 6, Issue 2, 1 March 1921, Pages 144–161, https://d.Missing: disassortative | Show results with:disassortative
[5]
[PDF] Systems of mating. V. General considerations. - ESP.ORG
The systems of breeding which have been considered, aside from random mating, are inbreeding of various sorts, assortative and disassortative mat-.
[6]
Population Genetics and Statistics for Forensic Analysts | Random ...
Jul 17, 2023 · Positive assortative mating leads to an increase in homozygotes whereas negative assortative mating leads to an increase in heterozygotes.
[7]
Assortative Mating and Linkage Disequilibrium | G3 Genes|Genomes
Jan 1, 2017 · Disassortative mating can be examined for this model in a similar ... correlation coefficient or.
[8]
Lect 3 Pop. Gen. I Intro.
Disassortative mating will tend to increase heterozygosity (put unlike alleles together) without affecting gene frequencies. Rare allele advantage. In some ...
[9]
Assortative Mating in Animals | The American Naturalist: Vol 181, No 6
Assortative mating occurs when there is a correlation (positive or negative) between male and female phenotypes or genotypes across mated pairs.
[10]
Scrutinizing assortative mating in birds - PMC - NIH
Feb 21, 2019 · The dotted line indicates no assortative mating (r = 0), negative r-values indicate disassortative mating, and positive r-values indicate ...
[11]
[PDF] The effect of assortative mating on the genetic ... - Gwern.net
Disassortative mating has op- posite effects, a decrease of homozygosity and variance and a building up of linkage disequilibrium in the opposite direction ( ...<|separator|>
[12]
[PDF] Frequency-Dependent Selection and the Evolution of Assortative ...
We describe the conditions under which sexual selection blocks or facilitates the evolution of higher levels of assortative mating. We turn now to a description ...
[13]
Assortative mating for reproductive timing affects population ...
Jan 20, 2023 · Generally, assortative mating can be expected to increase genetic variance among groups (by allowing for genetic divergence via reduced gene ...
[14]
(PDF) Evolution and genetic architecture of disassortative mating at ...
While the evolution of assortative mating with respect to locally adapted ... To test for assortative and disassortative mating, we paired females with ...Missing: seminal | Show results with:seminal
[15]
Effects of Genetic Architecture on the Evolution of Assortative Mating ...
Effects of Genetic Architecture on the Evolution of Assortative Mating Under Frequency-Dependent Disruptive Selection. Theor Popul Biol. 2011 May;79(3):82-96 ...
[16]
The Evolution of Mating Preferences and Major Histocompatibility ...
Here we provide a critical review of the studies on MHC‐dependent mating preferences in mice, sheep, and humans and the possible functions of this behavior.Mhc‐dependent Mating... · Mhc Genes: Immunological... · The Inbreeding Avoidance...
[17]
New perspectives on mate choice and the MHC | Heredity - Nature
Sep 1, 1998 · MHC mating preferences were reversed in males cross-fostered soon after birth to MHC-dissimilar parents, but remained disassortative in mice ...Missing: seminal | Show results with:seminal
[18]
Evolution of self‐incompatibility in the Brassicaceae - PubMed Central
Self‐incompatibility (SI) is a widespread mechanism to avoid selfing in flowering plants, whereby fertile plants are capable of self‐pollen recognition and ...
[19]
Cryptic preference for MHC-dissimilar females in male red ... - NIH
The degree of MHC similarity between two individuals was calculated using the following formula ... index of MHC similarity in the model with indices of ...
[20]
Evolution of MHC genetic diversity: a tale of incest, pestilence and ...
MHC-based disassortative mating preferences are presumed to have evolved because they reduce homozygosity throughout the genome, and particularly within loci ...
[21]
Disassortative mating for boldness decreases reproductive success ...
Aug 3, 2013 · Our results showed that female boldness did not affect mate choice, and brood size was independent of the boldness of the male and female in a ...
[22]
A statistical methodology for estimating assortative mating for ...
Jun 14, 2017 · These approaches include (1) the correlation between the mean phenotypes of males and females, (2) the correlation between randomized values of ...
[23]
MHC signaling during social communication - PMC - PubMed Central
MHC social signaling has been identified in over 20 species of vertebrates and is likely the basis for a vertebrate-wide chemosensory communication system. The ...
[24]
MHC-dependent mate preferences in humans - Journals
Here we show that the MHC influences both body odours and body odour preferences in humans, and that the women's preferences depend on their hormonal status.
[25]
Compatibility and incompatibility in S-RNase-based systems - NIH
Jul 28, 2011 · S-RNase-based self-incompatibility (SI) occurs in the Solanaceae, Rosaceae and Plantaginaceae. In all three families, compatibility is controlled by a ...
[26]
[PDF] Evolution of MHC Genes and MHC Gene Expression - uu .diva
MHC-based selective mating could be a mechanism to avoid inbreeding more generally, as MHC loci are highly polymorphic and individuals that share MHC alleles ...<|separator|>
[27]
Quantitative Analysis of Mouse Urine Volatiles: In Search of MHC ...
mice deficient in ß2M, MHC class I proteins, and CD8+T cells. Science 248 ... Previous studies of mating preference signified that mice can sense one ...
[28]
https://doi.org/10.1073/pnas.1702482114
[29]
MHC–disassortative mating preferences reversed by cross–fostering
Mice are able recognize MHC–similar individuals through specific odour cues. However, to mate disassortatively for MHC genes, individuals must have a referent, ...
[30]
Statistical analysis of assortative and disassortative mating in ...
Disassortative mating has also been observed, though it does not seem to follow from simple evolutionary or behavioural mechanisms. The obvious example of ...
[31]
[PDF] INBREEDING, OUTBREEDING, AND RANDOM MATING
Because nonrandom mating affects heterozygosity levels, it is useful to “quantify” the level of nonrandom mating by comparing the heterozygosity observed in ...<|control11|><|separator|>
[32]
Evolution and genetic architecture of disassortative mating at a locus ...
Nov 18, 2020 · We confirm that heterozygote advantage favors the evolution of disassortative mating and show that disassortative mating is more likely to ...
[33]
Mate Choice Contributes to the Maintenance of Shell Color ...
Whenever observed, a straightforward consequence of disassortative mating is that the polymorphism is protected from fixation or loss, independently of ...
[34]
Evolution and genetic architecture of disassortative mating at a locus ...
Sep 16, 2020 · Indeed disassortative mating favors the production of heterozygotes and reduces the expression of the genetic load in the offspring. In contrast ...
[35]
Correlations between heterozygosity and measures of genetic ...
Sep 23, 2005 · Disassortative mate choice may serve to avoid inbreeding (Pusey & Wolf, 1996) and increase heterozygosity in offspring, with consequent indirect ...Missing: formula | Show results with:formula
[36]
Assortative Mating and Linkage Disequilibrium - PMC - NIH
Assortative mating has been suggested to result in an increase in heritability and additive genetic variance through an increase in linkage disequilibrium.
[37]
Patterns of MHC-dependent mate selection in humans ... - PubMed
Our results indicate that preference for more MHC-diverse mates is significant for humans and likely conserved across primates.
[38]
Major histocompatibility complex-associated odour preferences and ...
Analysis of genomic studies reveals no association between MHC-dissimilarity and mate choice in actual couples; however, MHC effects appear to be independent ...
[39]
Mating preferences can drive expansion or contraction of major ...
Feb 26, 2020 · In consequence, preferences for MHC-dissimilar mates should result in improved immunity of progeny (although this benefit may be outweighed by ...
[40]
The importance of immune gene variability (MHC) in evolutionary ...
MHC variants influence many important biological traits, including immune recognition, susceptibility to infectious and autoimmune diseases, individual odours, ...
[41]
No evidence for a relationship between MHC heterozygosity and life ...
Jun 23, 2020 · More recent research has found evidence for a link between greater MHC heterozygosity and slower life history strategies in humans. This work is ...
[42]
Health, health behaviors, and health dissimilarities predict divorce
May 1, 2015 · Couples with similar health and health behavior are at a lower risk of divorce than are couples who are dissimilar in health. Health differences ...Missing: disassortative humans
[43]
Here are some actual facts about George Church's DNA dating ...
Dec 11, 2019 · So is Digid8 eugenics? Yes and no. Eugenics usually refers to forced sterilization, imposed breeding, or extermination of people by a state.Missing: MHC disassortative
[44]
https://doi.org/10.1098/rspb.1998.0433
[45]
Self-(In)compatibility Systems: Target Traits for Crop-Production ...
Self-incompatibility (SI) mechanisms prevent self-fertilization in flowering plants based on specific discrimination between self- and non-self pollen.Abstract · Introduction · Applications to Crop... · Future Prospects
[46]
Late‐acting self‐incompatibility – the pariah breeding system in ...
Jun 6, 2014 · This review draws attention to the confusion between species that show 'self-incompatibility' and those that possess one of the 'conventional SI mechanisms'.
[47]
S-RNase–mediated Gametophytic Self-Incompatibility is Ancestral in ...
Unlike the other systems, gametophytic self-incompatibility in grasses is controlled by two unlinked multiallelic loci, S and Z. Recent molecular work indicates ...
[48]
Mechanism of self/nonself-discrimination in Brassica self ... - Nature
Oct 1, 2020 · In Brassica, self/nonself-discrimination in the SI reaction is sporophytically controlled by a single locus, called S, with over 100 haplotypes.
[49]
The S locus glycoprotein and the S receptor kinase are sufficient for ...
Two S locus-encoded proteins are coordinately expressed in the stigma epidermis: the cell wall-localized S locus glycoprotein (SLG) and the plasma membrane- ...
[50]
Molecular insights into self-incompatibility systems: From evolution ...
Feb 12, 2024 · Here, we review current knowledge about the evolution of SI systems, summarize key achievements in the molecular basis of pollen‒pistil interactions,
[51]
Challenges and Perspectives in the Study of Self-Incompatibility in ...
Nov 29, 2021 · Self-incompatibility affects not only the formation of seeds, but also the evolution of species diversity. A robust understanding of the ...
[52]
Genetic Components of Self-Incompatibility in Brassica Vegetables
Feb 16, 2023 · Self-incompatibility (SI) is a common phenomenon in Brassica. Breeding by SI lines is an important way to utilize heterosis of Brassica vegetables.