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Palindromic sequence

A palindromic sequence in molecular biology refers to a deoxyribonucleic acid (DNA) segment that exhibits symmetry, reading the same sequence of nucleotide bases in the 5' to 3' direction on one strand as on the complementary strand when read in the 3' to 5' direction.^[1] This inverted repeat structure arises because the two halves of the sequence are complementary, allowing the DNA to potentially form a hairpin loop when single-stranded.^[2] Such sequences are prevalent across genomes of diverse species, from bacteria to humans, and often span short motifs of 6 to 20 base pairs, though longer variants exist.^[3] Palindromic sequences play crucial roles in gene regulation and DNA processing, serving as recognition sites for homodimeric proteins such as transcription factors, which bind symmetrically to control gene expression.^[4] They are also essential binding motifs for restriction endonucleases, enzymes used in molecular cloning to cut DNA at specific locations, enabling techniques like recombinant DNA technology.^[5] In viral genomes, such as those of retroviruses, palindromic elements facilitate RNA dimerization and replication initiation.^[6] Beyond their functional utility, palindromic sequences influence genome stability; imperfect or long palindromes can promote structural rearrangements, contributing to genomic instability and diseases including cancers, neuronal disorders, and intellectual disabilities.^[2] Their study has driven biotechnological advances, such as in CRISPR-Cas systems, which utilize clustered regularly interspaced short palindromic repeats to enable precise genome editing.^[7] Overall, these sequences underscore the structural elegance and functional versatility of DNA architecture.^[8]

Definition and Characteristics

Definition

A palindromic sequence in nucleic acids refers to a segment of double-stranded DNA or RNA in which the base sequence on one strand, read in the 5' to 3' direction, is identical to the sequence on the complementary strand, also read in the 5' to 3' direction.^[5] This inherent symmetry arises from the antiparallel orientation of the two strands, where each base pairs with its complement (adenine with thymine or uracil, and guanine with cytosine), resulting in a mirrored structure that facilitates specific molecular interactions.^[2] To illustrate, consider the sequence 5'-GAATTC-3' on one strand; its complementary strand is 3'-CTTAAG-5'. When the complementary strand is read from 5' to 3', it yields 5'-GAATTC-3', exactly matching the original sequence, confirming its palindromic nature.^[5] Such sequences are typically short and exhibit perfect or near-perfect symmetry without intervening spacers in their ideal form.^[3] Although palindromic sequences are predominantly discussed in the context of double-stranded nucleic acids due to their biological prevalence, they also occur in single-stranded RNA, where they enable intrastrand base pairing to form stable hairpin loops.^[9] The concept of a palindromic sequence was borrowed from linguistics—where it denotes a word or phrase that reads the same forwards and backwards—and first applied to DNA in the early 1970s amid investigations into the symmetrical recognition sites of restriction endonucleases.^[10]

Key Characteristics

Palindromic sequences in DNA are classified into several types based on their structural fidelity and arrangement. Perfect palindromes feature two identical inverted repeats positioned adjacent to one another, resulting in an exact match when the sequence is read in the 5' to 3' direction on one strand and compared to the reverse complement of the other strand.^[3] For instance, the sequence 5'-GGATCC-3' forms a perfect palindrome because its complementary strand reads 5'-GGATCC-3' in the 5' to 3' direction.^[11] Imperfect palindromes, also known as quasipalindromes, include mismatches or minor variations between the inverted repeats, disrupting full symmetry while retaining partial inverted complementarity.^[3] Interrupted palindromes consist of inverted repeats separated by non-palindromic spacers, which introduce gaps that prevent continuous symmetry.^[12] The length of palindromic sequences varies, but functional sites in biological contexts are typically 4-8 base pairs long, as this range allows for stable recognition without excessive rigidity.^[13] Longer palindromes, exceeding several dozen base pairs, are rare due to their propensity for instability, often leading to replication-dependent deletions or structural rearrangements during DNA processing.^[5] Notation for palindromic sequences conventionally represents the top strand in the 5' to 3' direction, with the complementary bottom strand indicated below it, also read 5' to 3', to highlight the inversion.^[11] This is often visualized using arrows (→ for 5' to 3' on one strand and ← for the reverse on the complement) or diagrams to denote the mirrored structure, emphasizing the dyad symmetry without altering the linear sequence presentation.^[14] Palindromic sequences occur ubiquitously across prokaryotic and eukaryotic genomes, appearing in both coding and non-coding regions.^[15] They exhibit a higher density in promoter regions, where short inverted repeats may facilitate regulatory interactions.^[2]

Structural Properties

Symmetry in Double-Stranded Nucleic Acids

In double-stranded nucleic acids, palindromic sequences exhibit dyad symmetry, characterized by inverted repeat motifs that create a twofold rotational axis, such that a 180-degree rotation around this axis aligns the complementary strands precisely.^[16] This symmetry arises from the base-pairing nature of DNA, where the sequence on one strand reads the same forward and backward when considering both strands together, forming a mirror-like structure across the dyad axis.^[17] Such configurations are prevalent in regulatory regions and enable efficient molecular interactions without requiring complex sequence variations.^[18] The dyad symmetry of palindromic sequences facilitates binding by symmetric proteins, particularly dimeric enzymes and transcription factors, which recognize the DNA through identical subunits contacting corresponding half-sites on either side of the axis.^[4] For instance, homodimeric proteins like the LexA repressor or FNR transcription factor utilize helix-turn-helix motifs to engage the symmetric elements, enhancing specificity and stability of the complex.^[19] This bilateral recognition allows the protein to impose its own symmetry onto the DNA, often inducing minor conformational changes that optimize contacts without disrupting the double helix.^[20] A representative example of such symmetric motifs occurs in bacterial operators, such as the lac operator in Escherichia coli, where the pseudo-palindromic inverted repeats serve as binding sites for the dimeric Lac repressor, enabling precise regulation of gene expression.^[20] The repressor subunits each interact with one half of the operator, leveraging the symmetry for high-affinity binding that responds to environmental signals like lactose availability.^[21] From an evolutionary perspective, the symmetry inherent in palindromic sequences represents a simple and efficient mechanism for encoding bilateral protein recognition across genomes, with these motifs showing conservation in functional regulatory elements due to their selective advantage in facilitating reliable molecular interactions.^[4] This conservation underscores how dyad symmetry has been maintained through natural selection to support essential processes like transcriptional control, minimizing the need for asymmetric adaptations in protein-DNA interfaces.^[22]

Formation of Secondary Structures

In single-stranded DNA or RNA, palindromic sequences facilitate the formation of hairpin loops, also known as stem-loop structures, through intramolecular base pairing between the inverted repeat regions.^[3] The self-complementary nature of these sequences allows the two halves to pair with each other, creating a double-stranded stem flanked by an unpaired loop at the center. This folding is particularly prominent in single-stranded contexts, such as during DNA replication on the lagging strand or in RNA molecules where palindromes contribute to regulatory elements.^[3] In double-stranded DNA, palindromic sequences can extrude into cruciform structures under conditions of negative supercoiling, resulting in an X-shaped configuration with two opposing hairpin loops.^[23] Negative supercoiling provides the torsional stress necessary to destabilize the canonical B-form helix, promoting the branch migration that separates the strands and allows intra-strand pairing within each arm of the palindrome. This extrusion is a dynamic process, often reversible, and is favored in sequences with perfect or near-perfect inverted repeats.^[24] The stability of these secondary structures is governed by thermodynamic factors, including the free energy of folding, which is lowered due to the self-complementarity inherent in palindromic sequences.^[25] Longer palindromic arms enhance stability by increasing the number of base pairs in the stem, while higher GC content further strengthens the structure through additional hydrogen bonding and base stacking interactions.^[25] Experimental evidence for cruciform extrusion has been obtained through techniques such as gel electrophoresis, which detects mobility shifts in supercoiled plasmids containing palindromic inserts due to the altered topology of the extruded form.^[26] Atomic force microscopy has provided direct visualization of these X-shaped structures on plasmid DNA surfaces, confirming the predicted dimensions of the hairpin arms in negatively supercoiled molecules.^[27]

Biological Functions

Recognition Sites for Restriction Enzymes

Palindromic sequences play a central role in prokaryotic defense mechanisms through restriction-modification (RM) systems, where type II restriction endonucleases recognize and cleave foreign DNA at specific palindromic sites, while corresponding methyltransferases modify the host's DNA to prevent self-cleavage.^[28] These type II enzymes typically identify symmetric DNA sequences of 4 to 8 base pairs and hydrolyze phosphodiester bonds within or near the recognition site in the presence of Mg²⁺ ions.^[13] The palindromic nature of these sites ensures that the double-stranded DNA is cleaved symmetrically, producing consistent fragments that distinguish invading nucleic acids, such as from bacteriophages, from protected host DNA.^[29] Classic examples include EcoRI, isolated from Escherichia coli, and BamHI, isolated from Bacillus amyloliquefaciens, two widely studied type II enzymes. EcoRI recognizes the palindromic sequence 5'-GAATTC-3' and cleaves between the guanine and adenine residues on both strands, generating 5' overhangs of AATT.^[30] Similarly, BamHI targets 5'-GGATCC-3' and cuts immediately after the first guanine on each strand, producing 5' overhangs of GATC.^[31] These cleavage patterns result in "sticky ends" that facilitate ligation in downstream applications, and the enzymes' specificity arises from their ability to bind the inverted repeat structure inherent to palindromes.^[28] The binding mechanism of these enzymes exploits the symmetry of palindromic sites, with most type II endonucleases functioning as homodimers where each subunit interacts with one half of the recognition sequence.^[29] This dimeric architecture allows for coordinated DNA distortion and cleavage, often involving a conformational change upon binding that positions catalytic residues near the scissile bonds.^[32] Variants such as isoschizomers, which recognize and cleave the same sequence identically, and neoschizomers, which recognize the same sequence but cleave at alternative positions (e.g., SmaI and XmaI both targeting CCCGGG but with differing overhangs), expand the toolkit while maintaining the core palindromic dependency.^[33] In biotechnology, palindromic recognition sites have been instrumental since the 1970s for precise DNA fragmentation in cloning vectors, enabling the construction of recombinant DNA molecules by generating compatible ends for ligation into plasmids like those derived from E. coli K12.^[34] This application revolutionized molecular biology, allowing researchers to insert foreign genes into host organisms for expression and analysis, with enzymes like EcoRI and BamHI becoming staples in protocols for gene isolation and vector engineering.^[35]

Sites for DNA Methylation

Palindromic sequences play a crucial role in DNA methylation, particularly through CpG dinucleotides, which are inherently symmetric in double-stranded DNA, allowing for methylation on both strands. In vertebrates, DNA methylation predominantly targets these palindromic CpG sites, where a methyl group is added to the C5 position of the cytosine residue, facilitating symmetric modification that ensures heritability during cell division.^[36] This symmetry is evident in specific contexts, such as the palindromic 5'-CCWGG-3' sequence, where both cytosines can be methylated, contributing to stable epigenetic marks.^[37] The primary enzymes responsible for this process are DNA methyltransferases (DNMTs), with DNMT1 serving as the maintenance methyltransferase that preferentially methylates hemimethylated CpG sites post-replication to propagate methylation patterns across generations. DNMT3A and DNMT3B, in contrast, establish de novo methylation at unmethylated CpG sites, often in conjunction with DNMT3L, to initiate patterns during development.00285-7.pdf) In mammals, these enzymes ensure that methylation at promoter-associated CpG islands—a hallmark of gene regulation—silences transcription by recruiting repressive complexes and inhibiting transcription factor binding.^[38] Biologically, CpG islands, defined as GC-rich regions with elevated CpG density (typically >60% GC and observed/expected CpG ratio >0.6), are often unmethylated in active promoters but become hypermethylated to repress genes in contexts like cancer and X-chromosome inactivation.^[39] Hypermethylation of CpG islands in tumor suppressor gene promoters, such as those of p16 and MLH1, is a common epigenetic alteration in human cancers, leading to gene silencing and promoting tumorigenesis across various tumor types. Similarly, during X-chromosome inactivation in female mammals, widespread CpG methylation on the inactive X chromosome reinforces transcriptional silencing, with hypermethylated CpG islands correlating with escape from inactivation only in specific pseudoautosomal regions.^[40] Evolutionarily, vertebrate genomes exhibit a significant depletion of CpG dinucleotides—observed at only about 20-25% of expected frequency—primarily due to the spontaneous deamination of 5-methylcytosine to thymine, which introduces C-to-T transition mutations over time.^[41] This mutability underscores the importance of the remaining CpG sites, particularly in protected CpG islands, which resist methylation and depletion to maintain regulatory functions, highlighting their conserved role in epigenetic control despite genomic pressures.^[39]

Palindromic Repeats in CRISPR Systems

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) are arrays found in the genomes of many bacteria and archaea, consisting of short direct repeats, typically 28-37 base pairs in length, that are often palindromic and separated by unique spacer sequences derived from foreign DNA, such as that of invading bacteriophages.^[42] These repeats exhibit partial or near-perfect palindromic symmetry, enabling the formation of stable hairpin structures in RNA transcripts, which are crucial for recognition and processing by CRISPR-associated (Cas) proteins.^[42] The spacers, acquired during previous encounters with phages, provide sequence-specific memory of invaders, allowing the system to mount an adaptive immune response.^[43] In the CRISPR-Cas mechanism, a long primary transcript (pre-crRNA) from the CRISPR array is processed into mature CRISPR RNAs (crRNAs), each containing a spacer flanked by partial repeat sequences. In type II systems, such as those involving Cas9, the repeat region base-pairs with a trans-activating CRISPR RNA (tracrRNA) to form a duplex with hairpin structures that facilitate recognition and binding by Cas9.^[44] The resulting crRNA-tracrRNA complex guides Cas9 to complementary target DNA sequences in invading nucleic acids, where cleavage occurs adjacent to a protospacer-adjacent motif (PAM), typically NGG for Streptococcus pyogenes Cas9.^[44] In type I systems, the palindromic repeats form hairpins processed by Cas6 endonuclease, loading crRNAs into the Cascade complex for interference. Types III and IV exhibit varying degrees of repeat palindrome symmetry, influencing hairpin stability and Cas protein interactions, though type IV systems are less characterized and often lack complete adaptation modules.^[42] The palindromic nature of CRISPR repeats is essential for the structural integrity of these hairpins, which serve as binding sites for Cas proteins during crRNA maturation and effector complex assembly, thereby enabling precise targeting and cleavage of foreign DNA.^[45] This RNA-guided mechanism provides bacteria with heritable, adaptive immunity against phages and plasmids.^[43] The discovery of CRISPR-Cas functionality in bacterial immunity laid the foundation for the CRISPR-Cas9 genome editing technology, first demonstrated in 2012 using the type II system from Streptococcus pyogenes, which has since revolutionized genetic engineering by enabling precise, programmable DNA modifications in diverse organisms.^[44]

Role in T Cell Receptor Diversity

Palindromic sequences play a critical role in V(D)J recombination, the somatic rearrangement process that assembles T cell receptor (TCR) genes in developing T lymphocytes within the thymus, enabling the immune system to recognize a vast array of antigens.^[46] This process involves the ordered joining of variable (V), diversity (D, for β and δ chains), and joining (J) gene segments, each flanked by recombination signal sequences (RSS).^[47] RSS are composed of a conserved palindromic heptamer (consensus sequence 5'-CACAGTG-3'), separated by a spacer of either 12 or 23 base pairs from a less conserved nonamer (consensus 5'-ACAAAAACC-3').^[48] The palindromic heptamer is essential for recognition and binding by the RAG1/RAG2 recombinase complex, which initiates site-specific DNA cleavage according to the 12/23 rule—recombination occurs only between a 12-spacer RSS and a 23-spacer RSS—to ensure sequential assembly of TCR chains.^[49] The mechanism begins with the RAG complex binding to the RSS heptamer, nicking the DNA at the junction between the heptamer and the adjacent coding segment via a hydrolytic reaction.^[50] This is followed by transesterification, where the 3'-OH from the nick attacks the opposite strand, forming a covalently closed hairpin structure at the coding end and a blunt signal end; the palindromic symmetry of the heptamer facilitates this hairpin formation by promoting strand alignment and synapsis between paired RSS.^[51] The hairpins are then opened asymmetrically by the Artemis nuclease, often resulting in short palindromic (P) nucleotides at the coding joints due to the overhang resolution, alongside non-templated nucleotide additions by terminal deoxynucleotidyl transferase (TdT) and exonucleolytic deletions.^[50] These modifications at the junctions, particularly in the complementarity-determining region 3 (CDR3), introduce significant sequence variability.^[52] This palindromic-dependent recombination generates an estimated 10^6 to 10^8 unique TCR variants per individual, providing the repertoire diversity necessary for effective adaptive immunity against pathogens.^[46] Mutations disrupting RAG1 or RAG2 function, which impair RSS recognition and cleavage, lead to severe combined immunodeficiency (SCID), characterized by profound T and B cell deficiencies and recurrent infections.^[53] For instance, hypomorphic RAG mutations can result in partial V(D)J activity, manifesting as Omenn syndrome with oligoclonal T cell expansions but limited diversity.^[54] Thus, the integrity of these palindromic elements is vital for ordered, high-fidelity TCR diversification during T cell development.

Implications and Analysis

Effects on Genome Stability

Palindromic sequences, particularly long ones exceeding 50 base pairs, pose significant risks to genome stability by forming secondary structures that interfere with DNA replication and repair processes. During replication, these sequences can extrude cruciform structures in double-stranded DNA or form hairpins in single-stranded regions, leading to replication fork stalling and slippage on the lagging strand. Such events promote genomic rearrangements, including deletions, duplications, and translocations, as the extruded structures are prone to cleavage by structure-specific endonucleases, resulting in double-strand breaks.^[2]^[3]^[55] In the human genome, short palindromic sequences (up to 40 bp) are abundant, numbering over 13 million, while longer palindromes (>40 bp) are less frequent at approximately 182,000, with those exceeding 500 bp being particularly rare—only a few dozen exist, such as the 618 bp palindrome on chromosome 3. These long palindromes act as hotspots for breakage due to their high potential for cruciform formation, and their scarcity reflects evolutionary pressures selecting against such instability-prone elements, as evidenced by their underrepresentation in coding regions and enrichment in introns and intergenic areas. Despite their rarity, these sites contribute disproportionately to mutational events, accounting for a significant portion of small insertions and deletions observed in genomic variation catalogs.^[2]^[56] Palindromic sequences are implicated in various diseases through their role in promoting aberrant rearrangements. In Charcot-Marie-Tooth disease type 1A (CMT1A), a 1.5 Mb duplication encompassing the PMP22 gene on chromosome 17p11.2 is mediated by flanking homologous repetitive extragenic palindromic (REP) sequences that facilitate unequal recombination, leading to PMP22 overexpression and demyelinating neuropathy in approximately 70% of CMT1 cases. In cancer, palindromes serve as fragile sites susceptible to breakage under replication stress, enabling palindromic amplifications via break-fusion-bridge cycles that drive oncogene amplification, such as ERBB2 (HER2) in breast cancer, and contribute to tumor progression and poor prognosis.^[57]^[3]^[2] To mitigate these threats, cellular repair pathways target palindromic structures, particularly cruciforms, which resemble Holliday junctions. These structures can be resolved by GEN1 resolvase through cleavage, though unresolved breaks may lead to translocations; however, improper repair can exacerbate instability. Additionally, helicases such as WRN unwind these extrusions to avert cleavage, channeling repair toward homologous recombination or non-homologous end joining to restore genome integrity.^[58]^[59]

Methods for Detection and Identification

Computational methods for detecting palindromic sequences primarily involve algorithms that scan nucleic acid sequences for regions where one strand is the reverse complement of the other, accommodating perfect, imperfect, and interrupted palindromes. The EMBOSS suite includes the 'palindrome' tool, which identifies inverted repeats by searching for self-complementary sequences with user-specified minimum and maximum lengths, gap tolerances, and mismatch allowances, making it suitable for both short restriction sites and longer genomic elements. Custom scripts and libraries, such as those implemented in Python or Perl using dynamic programming or suffix array approaches, enable efficient genome-wide searches by comparing sequence substrings to their reverse complements, often integrated into pipelines like Biopython for large-scale analysis. For instance, a method using the Longest Previous reverse Factor (LPrF) table has been developed to detect all reverse repetitions in linear time, improving efficiency over naive pairwise comparisons. Genome-wide catalogs have facilitated systematic identification, with the Human DNA Palindrome Database (HPALDB) compiling over 12 million entries of palindromes longer than 6 base pairs in the human reference genome, derived from exhaustive computational scanning. Similarly, a 2020 reference catalog analyzed palindromic sequences across the human genome and 1000 Genomes Project variants, revealing conservation patterns and structural variations through alignment-based detection. Tools like NeSSie extend this to approximate palindromes by incorporating Shannon entropy calculations for motif significance, allowing exhaustive searches in prokaryotic and eukaryotic genomes. Experimental approaches complement computational predictions by verifying structural or functional features of palindromes in vitro or in vivo. DNase I footprinting identifies protein-binding sites on palindromic DNA, such as transcription factor recognition sequences, by revealing regions of reduced nuclease sensitivity where proteins protect the DNA from cleavage, as demonstrated in studies of leucine zipper proteins binding bent palindromic motifs. For cruciform structures formed by extruded palindromes, specific probes like monoclonal antibodies (e.g., 2D3) enable detection through immunofluorescence, distinguishing cruciforms from other secondary structures in cellular extracts. Electron microscopy (EM) provides direct visualization of cruciforms in supercoiled plasmids, where palindromic regions appear as four-armed junctions under conditions favoring extrusion, confirming their presence in palindromic DNA fragments. Bisulfite sequencing targets methylated palindromes, converting unmethylated cytosines to uracils while preserving 5-methylcytosine, allowing PCR amplification and deep sequencing to map methylation patterns at single-base resolution in palindromic contexts, though palindromes can introduce false positives due to symmetric conversion artifacts. Other techniques, such as two-dimensional gel electrophoresis, detect cruciform extrusion by altered migration patterns in supercoiled DNA, sensitive to topological changes induced by palindromes. More recently, S1-END-seq (as of 2022) uses S1 nuclease and next-generation sequencing to detect single-stranded DNA regions associated with secondary structures like cruciforms at high resolution in vivo.^[60] Key challenges in detection include distinguishing functional palindromes, which interact with proteins or form stable structures, from incidental ones arising by chance in AT-rich regions, requiring orthogonal validation like functional assays alongside computational scoring for evolutionary conservation or motif enrichment. Handling imperfect palindromes with interruptions or mismatches demands flexible algorithms that balance sensitivity and specificity, as rigid criteria may overlook biologically relevant variants while tolerant searches increase false positives. Recent advances integrate next-generation sequencing (NGS) data for high-throughput identification across diverse organisms, as in the GAP-Seq method, which combines S1 nuclease treatment of genomic DNA to enrich for extruded palindromes followed by NGS, enabling genome-wide profiling of cruciform-prone sequences in bacteria and eukaryotes. This approach has been applied to uncover palindrome distributions in microbial genomes, facilitating comparative analyses without prior sequence knowledge.

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Oct 13, 2003 · V(D)J recombination is absolutely crucial for the adaptive immune response. In its absence, our immune system is compromised.
[47]
V(D)J Recombination: Mechanism, Errors, and Fidelity - ASM Journals
RSSs consist of conserved heptamer and nonamer elements, separated by 12 or 23 nucleotides of less conserved “spacer” sequence (Fig. 2). Efficient recombination ...
[48]
Minireview V(D)J Recombination: Site-specific Cleavage and Repair
A RSS is composed of two conserved sequences, a palindromic heptamer (5′CACAGTG3′) and an AT rich nonamer (5′ACAAAAACC3′), which are separated by a relatively ...
[49]
The architecture of the 12RSS in V(D)J recombination signal and ...
Dec 29, 2014 · The resulting models indicate that the 12RSS adopts a strongly bent V-shaped structure upon RAG/HMGB1 binding and reveal structural differences, ...
[50]
V(D)J Recombination: Mechanism, Errors, and Fidelity - PMC - NIH
Nov 1, 2016 · Hairpin opening occurs through the action of the Artemis endonuclease, which often cuts off-axis, resulting in short, single-stranded extensions ...
[51]
The architecture of the 12RSS in V(D)J recombination signal and ...
RSSs contain two well-conserved elements, a palindromic heptamer ... Despite its central role in hairpin formation, the mechanism by which the PC is assembled is ...
[52]
Systematic pattern analyses of Vδ2+ TCRs reveal that ... - Frontiers
Sep 26, 2022 · The diversity of these CDR3 regions is further amplified by the insertion of palindromic sequences ... TCRs provided by the V(D)J rearrangement.
[53]
RAG mutations in human B cell-negative SCID - PubMed
Six of 14 B- SCID patients tested were found to carry a mutation of the recombinase activating gene 1 (RAG-1), RAG-2, or both.
[54]
Partial V(D)J Recombination Activity Leads to Omenn Syndrome
We present evidence that a defect in the Rag-1 and Rag-2 genes is responsible for Omenn syndrome, a severe combined immunodeficiency with substantial numbers of ...
[55]
Long DNA palindromes, cruciform structures, genetic instability and ...
Long DNA palindromes pose a threat to genome stability. This instability is primarily mediated by slippage on the lagging strand of the replication fork ...<|control11|><|separator|>
[56]
Palindromes in DNA-A Risk for Genome Stability and Implications in ...
Mar 11, 2021 · Many palindromes are known as fragile sites in the genome, sites prone to chromosome breakage which can lead to various genetic rearrangements or even cell ...
[57]
Charcot-Marie-Tooth disease type 1A - MedLink Neurology
The 1.5 mB gene region is flanked by homologous repetitive extragenic palindromic (REP) sequences, which serves as substrates for the recombination step ...
[58]
Two sequential cleavage reactions on cruciform DNA structures ...
Mar 12, 2013 · Here we show that two sequential reactions that cleave the cruciform structures give rise to the translocation: GEN1-mediated resolution that cleaves ...