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Prolagus

Prolagus is an extinct of lagomorphs belonging to the Prolagidae, with a record spanning approximately 20 million years from the early (Neogene) to the late (Quaternary). The originated in and became widespread across , , and during the Neogene, representing one of the longest-lasting mammalian . Traditionally classified within the Ochotonidae (pikas), recent phylogenetic analyses based on suggest Prolagus may instead form an independent to Ochotonidae, potentially warranting its own status as Prolagidae. The is characterized by several distinctive anatomical features, including the absence of the third lower (M/3), which differentiates it from modern pikas in the genus Ochotona. Species of Prolagus exhibited considerable morphological variation, with dental and cranial adaptations reflecting their evolutionary history as insular endemics in some cases. Over 20 have been named for the genus; multiple species are recognized, including Prolagus aff. figaro, Prolagus figaro, and Prolagus bilobus, but the most well-known is Prolagus sardus, the , which inhabited the islands of and . This species underwent an anagenetic evolutionary lineage on these Mediterranean islands, evolving from earlier continental forms over time. Prolagus sardus is notable for its insular , achieving an average body mass of around 723 grams (ranging from 450 to 1113 grams), far larger than extant pikas, and displaying a slow life-history strategy with evidence of extended lifespan inferred from high rates of age-related in fossils. It served as a key prey for endemic predators like the Sardinian fox (Cynotherium sardous) and was likely hunted by early populations. The genus as a whole played significant ecological roles in its habitats, contributing to trophic dynamics similar to modern lagomorphs. The of Prolagus occurred gradually, with most continental species disappearing by the end of the Pleistocene, while P. sardus persisted into historical times, potentially until the (approximately 2760 calibrated years before present) or even the Roman era. The final of P. sardus is attributed to factors, including the of non-native predators, ecological competitors, and possibly pathogens following human colonization of the islands. Fossils of the genus continue to provide valuable insights into lagomorph , island , and the impacts of human activity on insular ecosystems.

Taxonomy and Phylogeny

Classification

Prolagus is a genus of extinct lagomorphs formally established by Pomel in 1853. The taxonomic placement of the genus remains debated, with some authorities classifying it within the monotypic family Prolagidae, while others consider it a subfamily (Prolaginae) of the Ochotonidae, the family that includes the living pikas (genus Ochotona). This classification reflects ongoing discussions about its morphological and phylogenetic affinities to modern lagomorphs. The type species is Prolagus oeningensis (König, 1825), known from deposits in . The genus has no major synonyms, as its original description by Pomel has endured without significant revisions since its inclusion in the 2005 edition of , which elevated Prolagidae to family status. The temporal range of Prolagus spans from the stage of the Early to the , encompassing a diverse array of occurrences across and adjacent regions.

Phylogenetic Position

Prolagus, an extinct of lagomorph, is estimated to have diverged from the leading to modern pikas (Ochotona) approximately 30 million years ago during the , based on analyses of from P. sardus. This divergence places Prolagus as a distinct branch within , separate from the two extant families, Ochotonidae and . The , P. oeningensis, exemplifies early members of the known from deposits in . The phylogenetic placement of Prolagus has been debated, with evidence supporting either inclusion within Ochotonidae as a stem-ochotonid or recognition of a separate family, Prolagidae, primarily due to unique dental traits such as the absence of a lower third , a trilobed second lower , and hypsodonty with continuously growing . These morphological distinctions, observed in specimens, suggest Prolagus occupied a specialized niche divergent from modern pikas, while cranial and postcranial features align more closely with ochotonids overall. A 2023 ancient DNA study from P. sardus specimens re-evaluated this position using mitochondrial genomes, recovering Prolagus as sister to Ochotonidae with moderate bootstrap support (66–73%), rather than deeply nested within it or closer to . This analysis challenges strict Ochotonidae assignment by reinforcing the case for Prolagidae as an independent family, while confirming basal lagomorph affinities and a divergence timeline consistent with origins. Fossil-based cladistic analyses of European material further support Prolagus as sister to modern pikas, highlighting its role in early lagomorph diversification across before insular in the Mediterranean. These phylogenies, incorporating dental and skeletal , underscore Prolagus's position outside the crown Ochotonidae, with migrations and radiations shaping its distribution from the onward.

Description

Physical Morphology

Prolagus species were small to medium-sized lagomorphs that closely resembled modern pikas (genus Ochotona) in overall , though many exhibited a more robust build due to evolutionary adaptations. Mainland forms, such as Prolagus figaro, had an estimated body mass of 398–436 g, reflecting a moderate increase over continental relatives like P. cf. calpensis (approximately 25% heavier). Insular species, notably Prolagus sardus from , displayed pronounced , with body masses averaging 723 g (ranging from 450 to 1113 g). These size variations were assessed using postcranial bones like the , , and as proxies, which provided reliable estimates for body mass in the genus. Body lengths for Prolagus species are inferred from skeletal remains to fall within 20–30 cm for most mainland taxa, comparable to but exceeding the 12.5–25 cm of extant pikas, with P. sardus reaching approximately 23–30 cm in head-body length due to insular . Articulated skeletons of P. sardus reveal a trunk length of approximately 16.5 cm from skull to , supporting estimates of total head-body length in this when accounting for limb contributions. External likely mirrored that of pikas, featuring a compact, rounded body with short limbs adapted for agile movement in rocky or forested terrains, though direct soft-tissue evidence is absent. Fur was presumably dense, suited to temperate Mediterranean climates, but no preserved details are available. Skeletal features distinguished Prolagus from modern pikas while retaining lagomorph characteristics. The included well-preserved , thoracic, , and sacral vertebrae in articulated specimens, contributing to a robust . Appendicular elements showed elongated hind limbs, evidenced by tibiofibulae and metatarsals (e.g., right metatarsal V at 13.0 mm in P. sardus), facilitating bounding and scrambling for evasion and foraging, with capabilities for digging. Forelimbs were proportionally shorter, with humeri measuring around 36.9 mm, underscoring a build optimized for quick, bounding gait and burrowing. These traits supported a herbivorous , with dental adaptations aiding folivory.

Dental and Skeletal Features

Prolagus species possess continuously growing incisors characteristic of lagomorphs, enabling persistent wear during feeding on abrasive vegetation. A distinctive feature of their dental formula is the absence of an independent lower third molar (m3), with the lower cheek tooth row instead comprising a fused complex of p4, m1, and m2, setting Prolagus apart from other lagomorphs including leporids, which retain a bi-lobed m3. The cheek teeth in Prolagus are high-crowned () and rooted yet ever-growing, featuring complex transverse ridges and folds that enhance the grinding surface for processing tough plant material. These adaptations, including increased (e.g., H/L ratios up to 4.83 in some ) and folding complexity (measured by indices like ), reflect progressive evolutionary refinements for efficient mastication of fibrous diets. The postcranial skeleton features a robust , supporting digging behaviors inferred from bone microstructure and proportions indicative of capabilities alongside elements. With an estimated body mass averaging 723 g (ranging from to 1113 g) in species like P. sardus, these skeletal traits align with a involving burrowing and . Fossil evidence from P. sardus reveals pathologies such as , particularly in joint-bearing elements, suggesting from repetitive locomotion and possibly extended lifespans in insular settings. Prevalence rates of this condition in large samples from sites like Cave indicate biomechanical loading on limbs adapted for and navigating rugged .

Diversity and Distribution

Species List

The genus Prolagus includes over 20 named , all known exclusively from fossil records across , with the majority described during the 19th and 20th centuries from localities in , , , , and other regions. These represent a diverse but entirely extinct lineage of lagomorphs, with P. sardus as the last surviving member, persisting into the on the islands of before going extinct in historical times. The reached peak diversity during the , when multiple contemporaneous coexisted across the Mediterranean and . Several are considered valid based on morphological and biostratigraphic distinctions, particularly in dental features like the third lower (p/3). The is Prolagus oeningensis (König, 1825), from deposits near Oeningen, . Other recognized valid species include P. calpensis (Major, 1905) from sites in (e.g., near ), P. imperialis (Mazza, 1987) from fissure fillings in , , and P. sardus (Wagner, 1829) from cave deposits in . Synonymy within Prolagus is common due to subtle morphological variations and fragmented fossils; for instance, P. apricenicus (Mazza, 1987), also from the of , , was initially described alongside P. imperialis but later distinguished as a separate junior based on size and patterns, though some overlap persists in attributions. Other junior synonyms include P. savagei (Berzi, 1970), often subsumed under P. calpensis in later revisions, and P. figaro depereti (López-Martínez, 1975), treated as a variant of P. bilobus in some contexts. P. osmolskae Fostowicz-Frelik, 2010, from , is considered a junior of P. bilobus.
SpeciesTime PeriodType LocalityAuthor and YearNotes
P. oeningensisOeningen, König, 1825Type species; widespread in early .
P. calpensisRosia Bay, ()Major, 1905Mainland Iberian form; some synonymy with P. savagei.
P. imperialis, Mazza, 1987Insular giant; distinguished from P. apricenicus.
P. sardusWagner, 1829Last species; island endemic, extinct in historical period.
Additional valid species from revisions include P. ibericus, P. bilobus, P. michauxi, and P. vasconiensis, primarily from Miocene-Pliocene sites in and , contributing to the genus's high Neogene diversity.

Geographic Range

Prolagus exhibited a widespread distribution across during the Neogene, spanning the Miocene and Pliocene epochs, with fossil evidence extending from the in the west through central regions including , , and . The genus achieved its maximum geographic extent during the Miocene-Pliocene transition, reflecting its adaptability to diverse continental environments. Over 20 species have been identified within the genus, contributing to its broad spatial occupation. Key fossil localities underscore this European prevalence, such as the middle site of Sansan in , where early representatives of the genus were documented, and Oeningen in , the type locality for a common species known from more than 150 sites across western and . occurrences further highlight the genus's persistence in , including sites near that preserve late assemblages. Beyond , Prolagus expanded into during the , with remains recovered from Moroccan localities like the Aït Kandoula Basin, indicating intercontinental dispersal facilitated by lowered sea levels. In Western Asia, the genus is recorded from and early deposits in , , such as the Çankırı Basin, extending its range eastward. A striking case of insular occurred on the Corsica-Sardinia microplate, where Prolagus sardus survived into the , representing one of the latest known occurrences of the genus in the Mediterranean region. This prolonged persistence on isolated landmasses contrasts with the genus's earlier continental dominance.

Evolutionary History

Origins and Diversification

Prolagus evolved from the ancestral genus Piezodus, a monospecific known from the to early of , through an anagenetic process involving adaptations such as rooted cheek teeth and the absence of the third lower molar. This transition occurred approximately 20 million years ago during the early , marking the emergence of Prolagus as a distinct lineage within the lagomorphs. The genus first appeared in the fossil record during the early (around 20 Ma), with initial records indicating an origin in continental forests. By the middle (Langhian to , ~16–11.6 Ma), Prolagus underwent early radiation, diversifying into multiple lineages across and as environmental shifts, including , promoted . This period saw the establishment of species like P. oeningensis, which became widespread, reflecting adaptations to varied woodland and open environments amid cooling climates and tectonic activity. estimates suggest the phylogenetic divergence of Prolagus from modern pikas (Ochotona) occurred around 30 million years ago, supporting its independent evolutionary trajectory within Ochotonidae or as a separate family (Prolagidae). The (5.3–2.6 Ma) represented the peak of Prolagus diversification, with up to nine coexisting species in some fragmented habitats across the Mediterranean periphery, driven by and in insular and continental refugia. A key event facilitating this radiation was the (~5.96–5.33 Ma), when desiccation of the Mediterranean created land bridges, enabling migration to islands such as and influencing subsequent insular . This crisis allowed Prolagus populations from the Italian mainland to disperse southward and eastward, contributing to the genus's broad distribution in , , and western .

Decline and Extinction

The mainland populations of Prolagus underwent extinction by the , approximately 0.1 million years ago, primarily driven by climate cooling and associated habitat loss from Pleistocene glaciations, as well as increased competition from expanding leporid species adapted to open grasslands. These environmental shifts favored leporids, which thrived amid the spread of grasslands, while Prolagus species, more reliant on forested or mixed habitats, declined without evidence of significant overhunting by early humans on the mainland. In contrast, the insular species Prolagus sardus persisted on into historical times, representing the last surviving member of the genus. Its extinction occurred during the (ca. 2760 calibrated years before present) or possibly into the Roman era (up to the 6th century AD), coinciding with human colonization and linked to anthropogenic factors including habitat alteration through and the introduction of non-native predators such as dogs and cats, as well as competitors like the (Rattus rattus). The latest records of P. sardus consist of subfossil bones from Sardinian archaeological sites associated with the Roman period, confirming its presence until at least the early centuries before final disappearance. This prolonged survival on islands highlights how isolation buffered Prolagus from mainland pressures until direct human interference intensified.

Ecology and Paleobiology

Habitat and Diet

Prolagus species occupied diverse habitats that evolved with climatic and geographic changes throughout their temporal range. In the , early forms preferred subtropical swamps, wetlands, and forested edges, environments analogous to those of the modern (Sylvilagus palustris) in the Everglades. By the Pleistocene, particularly for insular endemics like P. sardus on , preferred habitats were resource-limited island ecosystems with abrasive vegetation. As strict herbivores, Prolagus individuals maintained a of matter. Dental wear patterns indicate consumption of abrasive vegetation, likely incorporating from soil-adherent foliage in insular settings. Key adaptations included teeth with complex enamel ridges and enlarged occlusal surfaces (megalodontia) for efficient grinding and prolonged wear resistance against abrasive foods; the absence of a molar further streamlined processing of tough herbaceous material.

Life History Traits

Bone histology analyses of Prolagus sardus reveal a growth pattern characterized by rapid early development followed by a prolonged period of slower somatic . Juvenile specimens exhibit highly vascularized fibrolamellar , indicative of fast initial rates comparable to those observed in modern pikas (Ochotona spp.), which supports efficient body mass accumulation in the first year of life. This transitions to parallel-fibered and lamellar in subadults and adults, reflecting a deceleration in velocity after the initial phase, with somatic extending up to approximately three years. Periodic lines of arrested (LAGs), numbering –2 in juveniles and up to 8 in adults, mark annual pauses in deposition, suggesting a strong seasonal influence on rhythms tied to environmental fluctuations. Reproductive traits in Prolagus sardus appear adapted to insular conditions, with evidence pointing to delayed maturation and potentially reduced . Skeletal indicators show the onset of slower-growing around one year of age, though full skeletal maturity is not reached until about three years, exceeding allometric predictions of 10 months for similarly sized lagomorphs. occurs at a relatively large body size (55–60% of adult , approximately 375–425 g), implying larger, more developed that enhance juvenile survival but likely correspond to smaller sizes compared to mainland lagomorph relatives. The presence of LAGs supports the inference of seasonal breeding patterns, synchronized with periods of resource availability to optimize in a stable but resource-limited environment. Longevity estimates for Prolagus sardus derive from skeletochronological counts of LAGs in long bones, indicating a minimum lifespan of up to eight years—substantially longer than the allometric expectation of five years for a of its body size (around 700 g). This extended lifespan is evidenced by the accumulation of multiple annual growth lines in adult femora and humeri, allowing individuals to persist well into adulthood with continued, albeit minimal, . As an insular endemic, Prolagus sardus exemplifies a slower life-history strategy shaped by gigantism and reduced predation pressure, characteristic of K-selected adaptations in isolated ecosystems. The delayed maturation, prolonged growth, and increased longevity deviate from the faster-paced traits of continental lagomorphs, prioritizing survival and resource efficiency over high reproductive output in the absence of intense biotic interactions, such as those from the extinct Sardinian dhole. This pattern aligns with broader trends in island mammals, where low extrinsic mortality favors extended lifespans and fewer, larger offspring.

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