The Pliocene Epoch, spanning from 5.333 to 2.58 million years ago, is the second and final epoch of the Neogene Period within the Cenozoic Era, bridging the warmer Miocene and the onset of the Quaternary glaciation.[1] It is subdivided into the Zanclean Stage (5.333–3.600 Ma) and the Piacenzian Stage (3.600–2.58 Ma).[1] This epoch is defined by a global cooling trend that reduced atmospheric CO₂ levels from peaks around 425 ppm in the early Pliocene to preindustrial values by its close, alongside higher sea levels averaging 25 meters above modern levels due to reduced polar ice volumes.[2][3]During the Pliocene, major tectonic events reshaped global geography, including the closure of the Central American Seaway by the formation of the Isthmus of Panama around 3 million years ago, which redirected ocean currents and facilitated faunal exchanges between North and South America known as the Great American Biotic Interchange.[3] Continental configurations approached modern patterns, with the uplift of the Himalayas from the India-Asia collision and the rise of western North American mountain ranges like the Cascades and Sierra Nevada contributing to drier interior climates and the expansion of grasslands across continents.[3] Climate varied from warm, equable conditions in the early Pliocene—analogous to projected near-future warming—with reduced temperature gradients between poles and equator, to increasingly variable glacial-interglacial cycles in the late Pliocene that presaged the Pleistocene Ice Ages.[2][4]Life during the Pliocene saw the modernization of terrestrial ecosystems, with the proliferation of C4 grasslands supporting diverse grazing herbivores such as advanced perissodactyls (horses, rhinos) and artiodactyls (camels, deer), while all major modern orders and families of mammals were established by mid-epoch.[5] Marine environments featured coral reef expansions and diverse plankton, reflecting warmer oceans, and early hominins like Australopithecus emerged in Africa toward the epoch's end.[3] By the Piacenzian, flora and fauna closely resembled contemporary biodiversity, making the Pliocene a key analog for studying human adaptation to changing climates.[2]
Definition and Etymology
Geological Definition
The Pliocene Epoch constitutes the concluding epoch of the Neogene Period within the Cenozoic Era, encompassing a duration from approximately 5.333 to 2.58 million years ago (Ma). This placement reflects its position as the most recent subdivision of the Neogene, following the Miocene Epoch and preceding the Pleistocene Epoch of the Quaternary Period, as delineated in the International Chronostratigraphic Chart.[6]The lower boundary of the Pliocene is demarcated by the Miocene-Pliocene boundary, defined at the base of the Zanclean Stage, which signifies the termination of the Messinian salinity crisis through the reinundation of the Mediterranean Basin by Atlantic waters. The Global Boundary Stratotype Section and Point (GSSP) for this boundary is situated in the Eraclea Minoa section along the southern coast of Sicily, Italy, precisely at the base of the Trubi Formation, where a sharp lithological shift from evaporites to deep-marine marls occurs.[7] Astrochronological calibration of this boundary, achieved by correlating sedimentary cycles to orbital forcing parameters such as eccentricity and precession, yields the precise age of 5.333 Ma, primarily through analysis of Sicilian sections including the Capo Rossello composite.The upper boundary corresponds to the base of the Gelasian Stage, marking the onset of the Pleistocene Epoch at 2.58 Ma, as ratified by the International Commission on Stratigraphy (ICS). This definition integrates biostratigraphic markers, magnetostratigraphy, and astrochronology from the Monte San Nicola section in Sicily, Italy, though the numerical age aligns with global calibrations from Mediterranean records. The ICS chart, updated as of December 2024 with no substantive changes reported through 2025, confirms these boundaries and underscores the Pliocene's role in bridging late Neogene warmth toward Quaternary glacial inception.[6]
Etymology and Naming
The term "Pliocene" was coined by the British geologist Charles Lyell in 1833, derived from the Greek words pleíon (πλείων), meaning "more," and kainós (καινός), meaning "new," to denote strata containing a higher proportion of modern (extant) fossil species compared to the underlying Miocene deposits.[8][9] This nomenclature reflected Lyell's observation that these younger Tertiary layers showed an increasing resemblance to contemporary faunas, emphasizing a gradual continuity in life's history rather than abrupt changes. Lyell introduced the term in the third volume of his seminal work, Principles of Geology, where he subdivided the Tertiary period into Eocene, Miocene, and Pliocene based on the percentage of living mollusk species in fossil assemblages—approximately 3.5% for Eocene, 17% for Miocene, and over 90% for Pliocene.[10][11]Lyell's classification built upon earlier 18th-century efforts to systematize rock strata, particularly the foundational work of Italian geologist Giovanni Arduino, who in 1759 proposed a hierarchical division of geological formations into Primary (crystalline rocks with few fossils), Secondary (fossil-rich sedimentary layers), and Tertiary (superficial deposits with abundant modern shells).[12] Arduino's scheme, outlined in letters to academic colleagues, provided the initial framework for recognizing the Tertiary as a distinct post-Secondary era, influencing subsequent refinements by 19th-century geologists such as William Buckland and Roderick Murchison, who further delineated fossil-based chronologies.[13] These developments marked a shift toward biostratigraphy, where fossil content became a key criterion for correlating and naming geological units.Initially established as the uppermost division of the Tertiary period in Lyell's uniformitarian paradigm, the Pliocene's status evolved with advancing geochronology; in the late 20th and early 21st centuries, following ICS decisions starting in 1977 and formalized in 2004, the Tertiary was recognized as obsolete and replaced by the Paleogene and Neogene periods, with the Pliocene redefined as an epoch within the Neogene, spanning from approximately 5.33 to 2.58 million years ago.[9] This transition reflected broader International Commission on Stratigraphy efforts to standardize the geologic time scale based on global boundary stratotypes, while retaining Lyell's original etymological intent to highlight the "more recent" character of its biota.[14]
Stratigraphy and Chronology
Global Stages
The Pliocene Epoch, spanning approximately 5.333 to 2.588 million years ago (Ma), is formally subdivided into two global stages by the International Commission on Stratigraphy (ICS): the older Zanclean Stage and the younger Piacenzian Stage.[15] These stages represent standardized chronostratigraphic units that facilitate global correlation of rock sequences and geological events across the epoch. The boundaries are defined by Global Stratotype Sections and Points (GSSPs), which anchor the stages to specific stratigraphic sections with primary markers such as biostratigraphic datums and magnetostratigraphy.[15]The Zanclean Stage, the basal division of the Pliocene, extends from 5.333 Ma to 3.600 Ma.[16] Its GSSP is located at the Eraclea Minoa section on the southern coast of Sicily, Italy (37°23'30"N, 13°16'50"E), at the base of the Trubi Formation, approximately 10 meters above the top of the Messinian evaporites.[7] The stage boundary is defined near the top of magnetic polarity chronozone C3r, correlated via astronomical tuning and radiometric dating to 5.333 ± 0.005 Ma.[16] A defining characteristic of the Zanclean is the rapid reflooding of the Mediterranean Basin following the Messinian salinity crisis, an event known as the Zanclean megaflood, which breached the Gibraltar sill and restored marine connections with the AtlanticOcean around 5.33 Ma. This transgression is evidenced by a sharp lithological shift from evaporites to open-marine marls and the influx of Atlantic fauna, marking a global paleoceanographic reorganization.[17]The Piacenzian Stage, the uppermost division of the Pliocene, spans from 3.600 Ma to 2.588 Ma.[18] Its GSSP is situated at the Punta Piccola section on the southern coast of Sicily, Italy (37°17'20"N, 13°29'36"E), at the base of a beige marl bed (small-scale carbonate cycle 77), marked by the first influx of the planktonic foraminifer Globorotalia crassaformis.[19] The boundary is calibrated to 3.600 ± 0.005 Ma using integrated bio-, magneto-, and chemostratigraphy.[18] The Piacenzian is characterized by progressive global cooling trends, with intensified climate variability toward its close, culminating at the upper boundary with the onset of Northern Hemisphere glaciation (NHG) at 2.588 Ma, as marked by the base of the Pleistocene Series and the Gauss-Matuyama magnetic reversal.[20] This stage records a transition from relatively warm conditions to cooler, more seasonal climates, influencing terrestrial and marine ecosystems worldwide.[21]
Regional Subdivisions
In North America, the Pliocene epoch is primarily subdivided using North American Land Mammal Ages (NALMAs), with the latest Hemphillian and the Blancan stages encompassing the period from approximately 5.3 to 2.6 Ma. These correlate to the global Zanclean (early Pliocene) and Piacenzian (late Pliocene) stages, respectively, based on mammalian biostratigraphy and magnetostratigraphic data. The Hemphillian, which begins in the late Miocene and extends into the early Pliocene, concludes around 4.9 Ma at the Hemphillian-Blancan boundary, identified through paleomagnetic correlations in western U.S. basins such as those in Nevada and California. The Blancan stage then dominates the North American Pliocene record, spanning from about 4.9 Ma to 1.8 Ma, though its Pliocene portion ends at roughly 2.6 Ma, marking a transition characterized by faunal turnovers in terrestrial deposits across the continent.[22][23][24]In Europe, regional subdivisions of the Pliocene generally align with the global chronostratigraphic framework of the Zanclean and Piacenzian stages, facilitating straightforward correlations across the continent's marine and continental sequences. However, in northern Italy, the Tabianian stage was established as a local subdivision for lower Pliocene (Zanclean-equivalent) deposits, based on lithostratigraphic and biostratigraphic features in the Tabiano region, where marly limestones and foraminiferal assemblages define the interval from about 5.3 to 3.6 Ma. This stage, erected in the early 20th century, reflects Mediterranean marine transgressions following the Messinian salinity crisis and has been integrated into the global standard, though it highlights subtle regional variations in sedimentation rates and fossil content compared to central European sections.[25][26][27]In Asia, particularly in China, the Pliocene is correlated through Asian Land Mammal Ages, with the Nihowanian (or Nihewanian) stage representing a key regional unit spanning approximately 3.0 to 1.0 Ma, aligned primarily to the late Piacenzian and early Pleistocene via mammalian biostratigraphy from fluviolacustrine deposits in the Nihewan Basin of northern China. This stage encompasses late Pliocene to early Pleistocene faunal assemblages, including hipparionines and other herbivores, which provide biochronologic markers for correlating isolated continental sequences across East Asia, though precise boundaries rely on magnetostratigraphy tying them to the global polarity timescale. Earlier Pliocene subdivisions in China, such as the Gaoshandongian (early Pliocene, ~5.3-4.2 Ma) and Jingleiian (middle Pliocene, ~4.2-3.4 Ma), further refine regional correlations but show offsets from European marine stages due to differing terrestrial records.[28][29][30]Regional subdivisions of the Pliocene often exhibit discrepancies with the global standard, primarily arising from geographic isolation that fostered distinct biostratigraphic signals and incomplete stratigraphic records. For instance, in Australia, the Kalimnan stage, defined from marine and lagoonal deposits in southeastern regions like the Jemmys Point Formation, spans about 4.3 to 3.4 Ma and overlaps the early to middle global Pliocene (Zanclean-Piacenzian), but may extend to cover the full epoch in some areas, complicating direct correlations due to endemic molluscan and ostracod faunas that differ from Northern Hemisphere markers. These variations necessitate integrated approaches, such as combining mammalian, marine invertebrate, and paleomagnetic data, to resolve offsets and ensure robust global synchronization.[31][32][33]
Paleoclimate
Temperature and Glacial Cycles
The Pliocene epoch was characterized by a global climate warmer than present, with mean surface temperatures approximately 2–3°C higher than modern values.[34] This elevated warmth supported the persistence of boreal forests in polar regions during the early Pliocene (Zanclean stage, 5.3–3.6 Ma), where tree-ring and isotopic analyses of sub-fossil wood from Ellesmere Island indicate summer temperatures up to 8°C warmer than today, allowing mixed coniferous and deciduous forests to thrive north of the Arctic Circle.[35] These conditions reflect a reduced temperature gradient from equator to pole, with minimal permanent ice cover in high latitudes.[36]A peak of Pliocene warmth occurred during the mid-Pliocene warm period (mPWP, approximately 3.3–3.0 Ma), when global temperatures reached their highest levels, estimated at 2–4°C above pre-industrial baselines based on multiproxy reconstructions.[37] The Pliocene Research, Interpretation and Synoptic Mapping (PRISM) project, utilizing data from deep-sea sediment cores including foraminiferal assemblages and alkenone proxies, has reconstructed sea surface temperatures and land conditions for this interval, indicating significantly reduced polar ice volumes and consequent global sea levels 20–25 m higher than today.[38] These reconstructions highlight a period of relative climate stability compared to later Pleistocene variability, with amplified warming in high latitudes driving the expansion of temperate vegetation into polar areas.[39]Toward the late Pliocene (Piacenzian stage, 3.6–2.58 Ma), a gradual cooling trend emerged, culminating in the onset of permanent Northern Hemisphere glaciation around 2.7 Ma.[20] This transition is marked by the first significant influx of ice-rafted debris (IRD) in North Atlantic sediments, such as those from Ocean Drilling Program sites, signaling the development of continental ice sheets on Greenland and possibly North America.[20] The cooling reduced global temperatures by about 1–2°C from mid-Pliocene peaks and initiated low-amplitude glacial-interglacial cycles.[20]These late Pliocene cycles were paced by Milankovitch orbital forcing, particularly variations in Earth's obliquity (41-kyr periodicity) and eccentricity, which modulated summer insolation at high northern latitudes and influenced ice sheet growth and decay.[40] Oxygen isotope records from benthic foraminifera in deep-sea cores confirm the dominance of 41-kyr cycles during this intensification of Northern Hemisphere glaciation, prior to the mid-Pleistocene transition to 100-kyr dominance.[40] This orbital influence, combined with declining atmospheric CO₂ levels, facilitated the establishment of quasi-periodic climate oscillations.[41]
Atmospheric and Oceanic Influences
During the Pliocene epoch, atmospheric CO₂ concentrations ranged from approximately 350 to 450 parts per million (ppm), significantly higher than pre-industrial levels of around 280 ppm but showing a gradual decline toward the late Pliocene. This range is reconstructed from multiple proxies, including boron isotope ratios in foraminiferal calcite, which indicate elevated CO₂ levels driven by changes in ocean chemistry and carbon cycling, and stomatal indices in fossil leaves, which reflect plant physiological responses to higher atmospheric CO₂.[42] These elevated CO₂ levels contributed to a warmer global climate, influencing atmospheric circulation and ocean-atmosphere interactions, though the late Pliocene decline coincided with increasing Northern Hemisphere glaciation.Precipitation patterns during the Pliocene were markedly influenced by warmer tropical conditions, leading to enhanced monsoonal activity in regions such as Asia and Africa. In Asia, evidence from paleosols and lake sediments in the Tibetan Plateau and surrounding basins indicates intensified East Asian summer monsoon rainfall, attributed to stronger moisture convergence from a warmer Indian Ocean and expanded tropical convection.[43] Similarly, in Africa, proxy records from lacustrine deposits and soil carbonates suggest increased seasonal precipitation in the Sahel and East African rift valleys, driven by northward shifts in the Intertropical Convergence Zone under elevated tropical sea surface temperatures.[44] These changes reflect broader ocean-atmosphere coupling, where warmer tropics amplified the hydrological cycle and regional moisture transport.Key ocean gateway closures profoundly shaped Pliocene atmospheric and oceanic dynamics. The progressive closure of the Panama Isthmus between approximately 4 and 3 million years ago (Ma) restricted inter-oceanic exchange, leading to freshening of the Pacific and salinification of the Atlantic, which enhanced the Atlantic Meridional Overturning Circulation (AMOC) by increasing density gradients in the North Atlantic.[45] Concurrently, the Antarctic Circumpolar Current (ACC) strengthened throughout the Pliocene, as evidenced by neodymium isotope records from deep-sea sediments, promoting greater isolation of Antarctic waters and influencing global heat distribution through intensified Southern Ocean upwelling.[46] These tectonic reconfiguration events amplified ocean-atmosphere feedbacks, redistributing heat and moisture on hemispheric scales.Weather patterns in the Pliocene exhibited reduced seasonality in mid-latitudes due to the overall warmer climate and altered circulation, with proxy data from pollen assemblages and isotopic records indicating milder temperature contrasts between seasons in regions like Europe and North America.[39] Additionally, warmer sea surface temperatures (SSTs) in the tropics fueled the intensification of hurricanes, as model simulations and geological traces of storm deposits reveal decreased vertical wind shear and increased potential intensity, resulting in more frequent and powerful tropical cyclones across both hemispheres. These shifts in atmospheric dynamics underscore the Pliocene's role as an analogue for future warming scenarios, highlighting amplified storminess linked to ocean warming.
Paleogeography and Tectonics
Continental Drift and Configurations
During the Pliocene epoch, the global configuration of continents reflected the ongoing fragmentation of the ancient supercontinents Laurasia and Gondwana, with plate motions continuing to shape ocean basins and landmasses. The breakup of Laurasia, which had begun in the Mesozoic, was effectively complete by the Pliocene, as North America and Eurasia remained separated by the progressively widening Atlantic Ocean. This separation resulted from seafloor spreading along the Mid-Atlantic Ridge, with the ocean basin expanding at rates of approximately 2-2.5 cm per year throughout the epoch, contributing to the isolation of these northern continents from one another.[47]Remnants of Gondwana exhibited distinct drift patterns during this period. South America achieved its relative isolation as a continental block following the closure of the Central American Seaway by the formation of the Isthmus of Panama around 3-4 million years ago, which connected it to North America via land bridge while severing deep-water exchange between the Pacific and Atlantic Oceans. Concurrently, the Australian continent continued its northward drift toward the Indonesian archipelago at a rate of about 7 cm per year, narrowing the gaps in the Indonesian Throughflow passages and altering regional ocean gateways between the Indian and Pacific Oceans.[48][49]In the eastern hemisphere, convergence between the African and Eurasian plates drove significant tectonic adjustments. This ongoing collision, occurring at a rate of roughly 2 cm per year, facilitated the formation and stabilization of key Mediterranean straits, including the Strait of Gibraltar, which allowed the Zanclean deluge to refill the Mediterranean Basin early in the epoch after the Messinian Salinity Crisis. Additionally, the Arabian Plate's northward advance led to intensified collision with the Eurasian Plate, with convergence absorbing up to 650 km of relative motion since the late Oligocene, though much of the Pliocene deformation occurred along the Bitlis-Zagros suture zone.[50][51]Paleogeographic reconstructions, derived from paleomagnetic data and backward extrapolations of modern GPS plate velocities, provide precise latitudinal positions for key landmasses during the Pliocene. For instance, the Indian subcontinent, having collided with Eurasia earlier in the Cenozoic, had reached nearly its modern latitudinal position by the early Pliocene, with minor northward adjustments of less than 1° over the epoch. These models, integrating hotspot reference frames and seafloor spreading records, confirm that overall continental positions were very similar to today's, with latitudinal shifts generally under 2° for most plates during the epoch.[52][53][54]
Orogenic Events and Volcanism
The Alpine-Himalayan orogeny persisted through the Pliocene, characterized by ongoing collisional tectonics that drove continued uplift and exhumation across the Eurasian plate margin. In the Himalayas, thermochronological studies indicate exhumation rates of 1–5 mm/yr in the central and eastern sectors, reflecting sustained convergence between the Indian and Eurasian plates.[55] Similarly, in the Western Alps, low-temperature thermochronology reveals Pliocene rock uplift rates of approximately 0.1–0.5 mm/yr, associated with isostatic rebound and erosional unloading following Miocene compression. The Pyrenees experienced more subdued uplift during this period, with exhumation rates dropping to approximately 0.1 mm/yr, as post-orogenic relaxation dominated after earlier Miocene peaks.[56] These vertical movements reshaped regional topography, enhancing drainage networks and sediment flux into adjacent basins.Subduction along the western margins of the Americas fueled prominent volcanic arcs during the Pliocene, with the Cascade and Andean systems exemplifying calc-alkaline magmatism driven by oceanic plate descent. In the Cascades, subduction of the Farallon-derived plates (including the Juan de Fuca ridge) sustained andesitic to basaltic eruptions, building proto-stratovolcanoes and calderas amid a broad arc; volcanic output decreased from Miocene highs but remained significant, with episodes of high-volume dacitic activity. Remnants of the Columbia River Basalt Group, primarily Miocene in age, include late flows extending into the early Pliocene (around 6–5 Ma), representing flood basalt overflows linked to earlier back-arc extension. The Andean arc, propelled by Nazca platesubduction beneath South America, exhibited continuous volcanism along a 7,000-km chain, with Pliocene centers like those in the Central Andes producing andesitic stratovolcanoes and ignimbrites at rates influenced by slab geometry and crustal thickening.The East African Rift marked a key extensional regime in the Pliocene, initiating around 5 Ma as the African plate began to split under influence from the Afar mantle plume and distant plate forces. This rifting involved normal faulting and basaltic volcanism, forming grabens and half-grabens that progressed northward, contributing to the early opening of the Red Sea and Gulf of Aden through oblique extension. Localized alkali basalt fields emerged along rift margins, signaling asthenospheric upwelling.Globally, Pliocene volcanism lacked major Large Igneous Provinces, contrasting with earlier Cenozoic events, and instead featured dispersed, moderate-scale activity tied to plate boundaries and intraplate hotspots. The Iceland plume, active since the Paleogene, drove subaerial and submarine basaltic eruptions in the North Atlantic, with Pliocene output forming shield volcanoes and fissure swarms at rates of approximately 0.01–0.1 km³/yr, influencing regional seafloor spreading.
Terrestrial Ecosystems
Vegetation and Flora
During the Pliocene epoch (5.333–2.58 million years ago), terrestrial vegetation underwent significant transformations driven by global cooling, increasing seasonality, and declining atmospheric CO₂ levels, leading to a shift from predominantly closed-canopy forests toward more open landscapes in many regions. Isotopic analyses of pedogenic carbonates and phytoliths indicate that C₄ grasslands, which are more efficient in warm, arid conditions, expanded substantially, particularly in low- to mid-latitude areas of Africa, Asia, and North America. This expansion is evidenced by carbon isotope (δ¹³C) values in soil carbonates shifting toward more positive ratios, reflecting a greater proportion of C₄ biomass in ecosystems.[57][58]The rise of C₄ grasslands marked a pivotal ecological change, with coverage increasing from approximately 10% of terrestrial biomass in the early Pliocene to around 40% by the late Pliocene in regions like the North American Great Plains and East African rift valleys. This transition is supported by δ¹³C data from fossil soils and tooth enamel, showing a dietary shift in herbivores toward C₄ resources, as well as phytolith assemblages dominated by grass silica bodies characteristic of C₄ pathways. In East Africa, pedogenic carbonate records from the Baringo Basin reveal a progressive increase in C₄ dominance, linked to enhanced aridity and fire regimes that favored grasses over woody C₃ plants. Globally, this expansion contributed to habitat heterogeneity, with mixed C₃-C₄ mosaics becoming common, though full dominance of pure C₄ grasslands occurred later in the Pleistocene.[58][59]In higher latitudes, temperate broadleaf forests persisted and evolved in Europe and North America, characterized by deciduous trees adapted to seasonal climates. Pollen records from western Europe, such as those from the Iberian Peninsula, show dominance of mixed deciduous forests with Quercus (oak) and other Fagaceae, alongside conifers like Pinus, reflecting cooler, moister conditions than in tropical zones. In North America, broadleaf deciduous forests contracted southward due to cooling and uplift-induced rain shadows, but remained prominent in the eastern and southeastern regions, with sclerophyllous elements emerging in the southwest. These forests supported diverse understories but began yielding to prairie expansions as aridity increased.[60]Tropical rainforests in Africa and Asia maintained megathermal characteristics, with humid, evergreen canopies, though they experienced fragmentation in response to regional drying. In Southeast Asia, the emergence of dipterocarp-dominated forests is evident from late Miocene to Pliocene pollen records, where Dipterocarpaceae taxa increased in abundance, forming emergent layers in rainforests following their migration from African origins. African rainforests, centered in the Congo Basin, featured diverse angiosperm families like Moraceae and Arecaceae, with stable high rainfall supporting closed-canopy structures until late Pliocene arid pulses. In southern continents, megathermal floras persisted in refugia; Australian records indicate Nothofagus and laurel forests in eastern regions, while South American Amazonian sites show palm-rich evergreen forests, both indicative of warmer Pliocene climates before Quaternary cooling.[61][62]Key plant taxa during the Pliocene included the rise of modern genera that shaped contemporary ecosystems. Poaceae (grasses) proliferated globally, with C₄ lineages like Andropogoneae becoming integral to open habitats, as seen in phytolith and pollen evidence from multiple continents. Quercus diversified in temperate zones, with fossil leaves and pollen from European sites like Portugal's S. Pedro da Torre deposits confirming its expansion into mixed woodlands, adapting to seasonal droughts via deciduous habits. These taxa's proliferation underscores the Pliocene's role in assembling modern floras, with angiosperm dominance reaching near-current levels.[63][60]Pollen records provide direct evidence of these vegetational shifts, particularly trends toward aridification in the late Pliocene. In Australia, Nullarbor Plain pollen sequences show an early Pliocene reversal of late Neogenearidification, with expansion of mesic forests including Eucalyptus and Banksia replacing earlier sparse shrublands and woodlands, before renewed drying in the middle Pleistocene. Patagonian records from Argentina reveal a late Miocene onset of open steppes with Ephedra and Amaranthaceae, persisting through the Pliocene with minimal grass input until the Quaternary. These archives highlight regional variability, with isotopic correlations confirming moisture declines that amplified grassland expansion.[64][65]
Terrestrial Fauna and Evolution
During the Pliocene epoch, terrestrial mammalian faunas underwent significant radiations, particularly among proboscideans, with genera such as Anancus representing early elephant-like forms adapted to diverse habitats across Africa, Eurasia, and later the Americas.[66] These gomphotheres, characterized by their four-tusked dentition and browsing lifestyles, diversified in response to expanding woodlands and savannas, contributing to ecosystem engineering through vegetation clearance and seed dispersal.[67] Concurrently, perissodactyls (odd-toed ungulates) experienced a marked decline in diversity, with many lineages like early hipparions reducing in abundance as global cooling favored open grasslands over forested environments.[68] In contrast, artiodactyls (even-toed ungulates) saw a pronounced diversification, particularly among ruminants such as early bovids and cervids, which adapted to grazing with hypsodont teeth suited to abrasive vegetation.[69]A pivotal event in Pliocene faunal dynamics was the Great American Biotic Interchange, initiated around 3 million years ago with the emergence of the Panamanian land bridge, facilitating bidirectional migrations between North and South American landmasses.[70] North American carnivorans, including canids and felids, rapidly dispersed southward, preying on native South American herbivores and contributing to the decline of endemic marsupials and litopterns.[71] Conversely, South American xenarthrans such as armadillos and ground sloths successfully invaded northward, exploiting vacant niches in browsing and burrowing guilds, though overall asymmetry favored northern invaders with higher success rates.[72] This interchange reshaped continental biotas, accelerating evolutionary pressures through competition and predation.Avian evolution during the Pliocene solidified the establishment of most modern orders, with fossil records from Eurasia and North America documenting near-contemporary faunas to those of today, including diversification within Passeriformes and Charadriiformes amid habitat shifts.[73] Squamate reptiles, including lacertids and colubrids, adapted to emerging grasslands through morphological innovations like elongated bodies for rapid locomotion and cryptic coloration for open terrains, as evidenced by Pliocene assemblages in eastern Europe and North America.[74] These adaptations enabled squamates to thrive in increasingly arid, vegetated landscapes, filling insectivorous and small-vertebrate predator roles.Extinctions among Pliocene terrestrial fauna primarily affected late Miocene holdovers, with gomphotheres like Cuvieronius and Stegomastodon showing reduced diversity toward the epoch's end, particularly in South America where immigrant competition and habitat fragmentation played key roles.[75]Fossil evidence from North American sites, such as those in the Great Plains, reveals a contraction of proboscidean populations as grasslands expanded, displacing browsing specialists.[76] This turnover set the stage for Pleistocene configurations, with surviving lineages reflecting selective pressures from climatic aridity.
Marine Environments
Oceanic Circulation and Chemistry
During the Pliocene epoch, the thermohaline circulation underwent significant reorganization, particularly with the strengthening of the Atlantic Meridional Overturning Circulation (AMOC) following the progressive closure of the Central American Seaway (CAS) around 4.6 Ma. This closure reduced the influx of low-salinity Pacific waters into the Atlantic, enhancing the density gradient and promoting more vigorous North Atlantic Deep Water (NADW) formation, as evidenced by increased overflow of dense waters across the Iceland-Scotland ridge by approximately 3.6 Ma.[77] The intensified AMOC facilitated greater heat transport to the North Atlantic, contributing to regional warming and influencing global climate patterns.[78]Upwelling systems along eastern ocean boundaries also intensified during the Pliocene, driven by strengthened coastal winds and trade wind systems associated with a steeper equator-to-pole temperature gradient in some regions. In the Benguela Current system off southwestern Africa, enhanced upwelling is indicated by increased biogenic silica accumulation and shifts in sediment proxies, reflecting higher nutrient delivery to surface waters in the late Pliocene.[79] Similarly, the California Currentupwelling zone showed amplified activity in the late Pliocene, as recorded in siliceous microfossil assemblages and productivity indicators from marginal sediments, leading to elevated primary production in the eastern Pacific.[80] These dynamics were modulated by orbital forcings but generally trended toward greater intensity compared to late Miocene conditions.[81]Seawater chemistry during the Pliocene featured distinct salinity gradients, as reconstructed from δ¹⁸O records in planktonic foraminifera, which reveal a small but variable inter-oceanic difference between the eastern tropical North Pacific and the Caribbean, peaking around 3.5–4 Ma due to CAS restriction.[82] Oxygenation levels in the oceans were generally higher than present-day values, with reduced extent of oxygen minimum zones inferred from benthic foraminiferal assemblages and redox-sensitive trace metals, particularly in the eastern Pacific and Indian Ocean margins.[83] This enhanced ventilation is attributed to stronger circulation and warmer temperatures that limited deoxygenation despite higher metabolic demands in a greenhouse climate.[84]The reduction of the Indonesian Throughflow around 4 Ma marked a key gateway effect, diminishing the exchange of warm, low-salinity waters between the Pacific and Indian Oceans due to tectonic uplift in the region. This constriction altered surface and deep circulation patterns, strengthening the Indian Ocean's anticyclonic gyre and contributing to aridification in adjacent landmasses through changes in moisture transport.[78] Overall, these oceanic changes had feedbacks on global climate stability, including modulated heat redistribution.[78]
Marine Life and Biodiversity
During the Pliocene epoch, planktonic foraminifera played a key role in marine ecosystems, with lineages of the genus Globorotalia dominating tropical and subtropical assemblages. The Globorotalia tumida lineage, in particular, exhibited punctuated gradualism in its evolution, showing rapid morphological transitions and increased test sizes as an adaptive response to environmental changes, such as cooling ocean temperatures toward the late Pliocene.[85] Similarly, calcareous nannoplankton of the genus Discoaster reached peak abundances in the mid-Pliocene, with species like Discoaster asymmetricus displaying cyclic variations tied to orbital forcing and warmer sea surface conditions that favored their proliferation in open ocean environments.[86] These microfossils contributed significantly to the calcium carbonate deposition in deep-sea sediments, influencing global carbon cycling.[87]Benthic communities in the Pliocene oceans showed notable expansions and diversifications. Deep-sea corals, including Lophelia pertusa, proliferated in regions like the Mediterranean Basin, where recolonization began in the early Pliocene after the Messinian salinity crisis, forming initial frameworks for cold-water coral mounds at depths of 200–1,000 meters.[88] On continental shelves, bivalves of the family Veneridae exhibited notable diversity, with multiple genera adapting to shallow, nutrient-rich habitats influenced by coastal upwelling and sea-level fluctuations; this is evidenced by fossil assemblages from the Mediterranean and eastern Atlantic.[89] These benthic groups supported complex food webs, with venerid bivalves serving as infaunal engineers that stabilized sediments and enhanced habitat heterogeneity.[90]Marine mammal diversity expanded markedly during the Pliocene, particularly among odontocetes. Toothed whales (Odontoceti) experienced a rapid radiation in the early Pliocene, driven by restructuring of ocean currents and the closure of the Central American Seaway, which promoted ecological divergence and the emergence of modern families like Delphinidae and Ziphiidae.[91] This radiation resulted in heightened morphological disparity, with adaptations for echolocation and diverse feeding strategies filling new niches in productive coastal and pelagic zones. Sirenians, such as dugongids, were prominent in coastal Indo-Pacific waters, where they grazed on seagrasses in shallow, tropical embayments; their distribution reflected the persistence of warm, vegetated nearshore habitats until the late Pliocene.[92]Reef systems in the Pliocene tropics were characterized by robust development of zooxanthellate corals, which constructed extensive fringing reefs along continental margins and islands. These symbiotic corals, reliant on dinoflagellate algae for energy, thrived in stable, warm waters (typically 25–30°C), building structures up to several kilometers in length in regions like the Indo-Pacific and Caribbean; tectonic stability and moderate sea-level rise facilitated their growth without widespread disruption.[93]Fringing reefs supported high biodiversity, including diverse fish and invertebrate assemblages, and acted as barriers against erosion while contributing to regional carbonate platforms. Ocean chemistry, with pCO₂ levels comparable to modern values (~400 ppm) but sufficient saturation states for calcification, supported these reef-building processes without the severe acidification seen in modern analogs.[94]
Human Evolution Context
Early Hominin Developments
The Pliocene epoch (5.3–2.58 Ma) marks a pivotal period in hominin evolution, with the emergence of species exhibiting key adaptations toward bipedalism and terrestrial foraging. Ardipithecus ramidus, dated to approximately 4.4 Ma, represents one of the earliest known potential hominin precursors, discovered in the Afar region of Ethiopia. Fossils of this species, including the partial skeleton known as "Ardi," reveal a combination of arboreal and bipedal traits, such as a pelvis adapted for upright walking alongside grasping toes for climbing, suggesting a mosaic of locomotor behaviors in wooded environments.Australopithecus afarensis, flourishing from about 3.9 to 2.9 Ma, provides more definitive evidence of habitual bipedalism among early hominins. The species is best represented by the "Lucy" skeleton (AL 288-1), a 3.2 Ma partial female recovered from the Hadar Formation in Ethiopia, which includes over 50% of the bones and demonstrates a fully bipedal lower limb with a curved phalanges indicating retained climbing ability. Additional fossils from Hadar, such as the "First Family" assemblage (A.L. 333), comprising remains of at least 13 individuals, further illustrate sexual dimorphism and group behaviors in this species. Anatomical features like the ilium-flared pelvis and valgus-angled femur in A. afarensis confirm efficient striding bipedalism, while the Laetoli footprints in Tanzania—three parallel trails dated to 3.66 Ma—preserve direct evidence of human-like heel-to-toe gait patterns, predating most skeletal indicators.[95]Early hominins during the Pliocene relied primarily on foraging for plant and small animal resources, with no widespread evidence of systematic tool use until the late epoch. The oldest confirmed stone tools, from Lomekwi 3 in Kenya (3.3 Ma), consist of simple flakes and cores produced by intentional knapping, indicating rudimentary percussive technology possibly linked to food processing. However, such artifacts remain rare and are not associated with A. afarensis sites like Hadar or Laetoli, where dental microwear and isotopic analyses suggest a diet dominated by C3 plants and occasional C4 resources, without reliance on tools for butchery. These developments occurred amid broader mammalian radiations in East African rift valleys, but hominins uniquely evolved obligate bipedalism as an energy-efficient adaptation to fragmented forest-grassland mosaics.[96]
Environmental Influences on Ancestors
The Pliocene epoch (5.33–2.58 million years ago) marked a period of progressive global cooling and aridification in eastern Africa, driven primarily by tectonic uplift associated with the East African Rift System and orbital variations in Earth's climate. This environmental shift transformed landscapes from predominantly wooded and humid environments to more open, mosaic habitats with expanding C4 grasslands. Paleoecological proxies, such as carbon isotopes in pedogenic carbonates and tooth enamel from fossil mammals, indicate that C4 vegetation increased significantly around 5–4 Ma in regions like the Turkana Basin, reflecting a decline in moisture availability and a rise in seasonality. These changes challenged early hominins, including Australopithecus species, by altering resource distribution and promoting adaptations for exploiting diverse habitats.[97][98]Aridification intensified toward the late Pliocene (3–2.6 Ma), coinciding with the emergence of the genus Homo and early stone tool use, as evidenced by faunal turnovers and pollen records from sites like Shungura and Koobi Fora in Ethiopia and Kenya. Leaf wax biomarkers and phytolith analyses reveal abrupt pulses of aridity linked to orbital eccentricity cycles every ~400,000 years, leading to episodic expansions of grasslands interspersed with gallery forests along rivers. This variability likely selected for behavioral flexibility in hominins, such as increased mobility and scavenging, while faunal shifts toward more open-adapted species (e.g., equids and antelopes) suggest competitive pressures that influenced dietary evolution from folivory to more granivorous and omnivorous patterns. High temperatures, averaging 2–4°C warmer than today in East African rift valleys, further stressed water resources, potentially driving innovations in thermoregulation and social structures among ancestral populations.[99][100][101]The mosaic nature of these Pliocene environments—combining woodlands, grasslands, and wetlands—provided refugia that buffered extreme aridity, allowing early hominins to persist and diversify without necessitating a complete shift to open savannas, contrary to earlier "savanna hypothesis" models. Stable isotope data from bovid teeth across multiple East African sites show that hominins occupied these heterogeneous landscapes, with evidence of continued reliance on C3 resources even as C4 grasses dominated. This environmental heterogeneity is posited to have fostered cognitive and technological advancements, as seen in the ~2.8 Ma appearance of Oldowan tools amid heightened climatic instability, ultimately shaping the trajectory toward modern human ancestry. Orbital-scale variability, rather than monotonic drying, emerges as a key driver, promoting speciation and extinction events in hominin lineages.[102][103][104]