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Rosavin

Rosavin is a phenylpropanoid and characteristic primarily isolated from the roots and rhizomes of L., a perennial flowering plant in the family traditionally used as an in Asian and European folk medicine to combat fatigue, stress, and environmental stressors. With the molecular formula C<sub>20</sub>H<sub>28</sub>O<sub>10</sub> and a structure featuring a backbone linked to glucose and moieties, rosavin contributes to the plant's pharmacological profile and commercial extracts of R. rosea are often standardized to at least 3% total rosavins (including rosavin as the primary component) and 1% . Rosavin exhibits a range of preclinical biological activities, including potent antioxidant effects by scavenging reactive oxygen species such as DPPH radicals (IC<sub>50</sub> = 5.86 µg/mL) and enhancing endogenous enzymes like superoxide dismutase (SOD) and catalase (CAT) in animal models. It also demonstrates antitumor properties, such as inhibiting proliferation of small cell lung cancer cells (at 5-100 µM) via induction of apoptosis, G<sub>0</sub>/G<sub>1</sub> cell cycle arrest, and suppression of the MAPK/ERK pathway, as well as reducing neovascularization in tumor models. Additional effects include immunomodulation by promoting B- and T-lymphocyte proliferation and modulating TRAIL signaling through ERK pathways, analgesic activity via NO/cGMP/K<sup>+</sup> and 5-HT<sub>1A</sub> receptor mechanisms in rodents, and anti-inflammatory benefits, such as mitigating ferroptosis and cytokine release in rat models of lung injury (at 100 mg/kg). Therapeutically, rosavin shows promise in addressing chronic conditions, including neuroprotective effects against cognitive disorders and by reducing and , gastroprotective and hepatoprotective actions in digestive and models, osteoprotective benefits for bone health, and pulmoprotective roles in respiratory diseases. Its content in R. rosea varies from 0.08% to 0.6% depending on factors like plant age, time, and , with extraction methods such as (yielding up to 969.71 mg/100 g at 70-75% ) and natural deep eutectic solvents enabling purification to over 98% purity for pharmaceutical applications. Despite these attributes, rosavin's high water solubility leads to low oral , and while preclinical data are robust, clinical trials are needed to confirm , , and optimal dosing in humans.

Chemical Properties

Structure and Formula

Rosavin is a phenylpropanoid glycoside and a characteristic marker compound in extracts of Rhodiola rosea. It consists of a trans-cinnamyl alcohol aglycone linked via a glycosidic bond to a disaccharide comprising β-D-glucopyranose and α-L-arabinopyranose. The glucose unit is substituted at its C-6 hydroxyl group with the arabinose moiety, forming the diglycoside structure that distinguishes rosavin from related compounds like rosin and rosarin. The molecular formula of rosavin is \ce{C20H28O10}, corresponding to a of 428.43 g/mol. Its systematic name is (2E)-3-phenylprop-2-en-1-yl 6-O-α-L-arabinopyranosyl-β-D-glucopyranoside. The full IUPAC name, reflecting the ring , is (2R,3R,4S,5S,6R)-2-[(E)-3-phenylprop-2-enoxy]-6-[[(2S,3R,4S,5S)-3,4,5-trihydroxyoxan-2-yl]oxymethyl]oxane-3,4,5-triol. The structural diagram of rosavin highlights the phenylpropanoid backbone—a trans-configured 3-phenylprop-2-en-1-yl chain—etherified to the β-anomeric oxygen of the glucopyranose. The arabinopyranose attaches to the at C-6 of glucose via an α-glycosidic linkage, resulting in a branched with multiple hydroxyl groups contributing to its . This arrangement is depicted as follows in simplified schematic form:
  • Central: β-D-Glucopyranose ring
  • Attached at C-1 (β): O-CH₂-CH=CH-Ph (trans-cinnamyl)
  • Attached at C-6: O-α-L-Arabinopyranose ring
The at key chiral centers ensures the specific β and α configurations of the glycosidic bonds.

Physical Characteristics

Rosavin is typically isolated as a to in its pure form. The compound demonstrates high solubility in polar solvents, including (≥5 mg/mL when warmed), , , and DMSO, reflecting its hydrophilic character and low oil- ; it is insoluble in non-polar solvents such as . Rosavin exhibits a melting point of 171–173 °C. It shows sensitivity to acidic conditions, where can occur, and is recommended for storage at −20 °C to preserve stability. Spectroscopically, rosavin displays a key UV absorption maximum at 254 nm, arising from its phenyl ring system, and characteristic ¹H NMR signals for glycosidic protons in the 3.0–5.0 ppm range.

Occurrence and Sources

Natural Plant Sources

Rosavin is primarily sourced from the roots and rhizomes of L., a perennial succulent herb in the family native to cold, high-altitude environments. This species thrives in rocky, alpine terrains and subarctic zones, where it exhibits strong adaptability to harsh conditions such as low temperatures and poor soils. Rhodiola rosea is distributed across the , including the regions of Europe (such as and the ), (notably and mountainous areas of at elevations of 1600–3100 m), and (including , , , and ). It prefers well-drained, gravelly slopes and cliff edges in non-polluted, mountainous habitats. Secondary natural sources of rosavin include Rhodiola sachalinensis A. Bor., another Rhodiola species containing the compound in lower amounts, primarily distributed in East Asian alpine and forested slopes of northeastern China (Jilin and Heilongjiang provinces), the Russian Far East (including Sakhalin and Kuril Islands), Japan, and the Democratic People's Republic of Korea. Rosavin is a diagnostic compound unique to these two Rhodiola species and is not found in other members of the genus. Historically, rosavin has been obtained through wild harvesting of and related species, a practice rooted in across these regions; however, intensive exploitation has led to population declines, prompting increased cultivation efforts to prevent overharvesting and ensure sustainable supply. Rosavin co-occurs in these plants with related phenylpropanoid glycosides, such as rosarin and .

Content Variation

The concentration of rosavin in the dry root and rhizome weight of Rhodiola rosea typically ranges from 0.5% to 3%, comprising part of the total rosavins (rosavin, rosarin, and rosin), though rosavin values up to 3.7% and total rosavins up to 5% have been reported in select cultivated samples. Standardized extracts for commercial and medicinal use generally require at least 3% total rosavins to ensure consistent potency and authenticity. Rosavin levels increase with plant age, reaching higher concentrations in 3- to 5-year-old individuals compared to younger , with peaks observed at maturity around 4 years in cultivated specimens from regions like . For instance, 4-year-old of the Altai Mountains exhibited 2.64% rosavin in rhizomes, significantly exceeding levels in 2- or 3-year-old counterparts. Environmental factors markedly influence rosavin accumulation, with elevated levels in plants grown under high-altitude conditions (e.g., 1580–2000 m above ) exposed to stressors like UV radiation and cold temperatures, which promote production as an adaptive response. In contrast, lowland or greenhouse-cultivated specimens at elevations below 200 m often show reduced concentrations, with rosavin content sometimes falling below 1% due to milder conditions limiting biosynthetic upregulation. Differences in species provenance and chemotypes further contribute to variation, as strains of R. rosea (e.g., from or South ) typically exhibit higher rosavin levels (up to 3.1%) than Asian variants from regions like the , where predominates and rosavin may be as low as 0.03%. Seasonal harvesting timing affects accumulation, with traditional practices favoring autumn for overall yield, though some studies indicate peaks in or early vegetative stages for maximum phenylpropenoid content in -cultivated plants.

Biosynthesis

Pathway Overview

The biosynthesis of rosavin originates from , an synthesized via the in . This pathway integrates phosphoenolpyruvate and erythrose-4-phosphate to form , the branch-point intermediate for production. From , the primary route in proceeds through prephenate (via chorismate mutase) to arogenate (via prephenate aminotransferase), followed by dehydration to yield , providing the foundational precursor for downstream phenylpropanoid metabolites like rosavin. In the phenylpropanoid branch, undergoes to produce , which serves as the core structure for subsequent modifications. Activation occurs through formation of cinnamoyl-CoA, followed by sequential reductions to and then to , the aglycone backbone of rosavin. This reduction pathway channels the molecule toward formation specific to certain adaptogenic plants. The final stages involve glycosylation of cinnamyl alcohol, where a β-D-glucose unit is first attached to form rosin, followed by the addition of an α-L-arabinopyranosyl unit at the 6-position of the glucose via a 1→6 glycosidic bond to complete rosavin. Overall, the biosynthetic scheme can be summarized as: phenylalanine → cinnamic acid → cinnamoyl-CoA → cinnamaldehyde → cinnamyl alcohol → rosin → rosavin. Key enzymes in these conversions are detailed elsewhere. This pathway is predominantly active in the Rhodiola genus, particularly , where rosavin accumulation is induced under conditions such as limitation, , or environmental stressors, enhancing the plant's adaptogenic response. occurs spontaneously in roots and rhizomes, contributing to the species-specific chemical profile of these high-altitude perennials.

Key Enzymes

The biosynthesis of rosavin, a cinnamyl alcohol glycoside characteristic of Rhodiola rosea, relies on several key enzymes in the phenylpropanoid pathway that convert phenylalanine into the aglycone cinnamyl alcohol, followed by glycosylation to rosin and then to rosavin. Phenylalanine ammonia-lyase (PAL) catalyzes the initial, rate-limiting deamination of phenylalanine to trans-cinnamic acid, committing the substrate to the phenylpropanoid branch and setting the flux for downstream rosavin production. This step is crucial as it initiates the formation of the cinnamyl backbone essential for rosavins. Subsequent activation occurs via 4-coumarate:CoA ligase (4CL), which ligates trans-cinnamic acid to , forming cinnamoyl-CoA for further modification in the pathway leading to rosavin. Cinnamoyl-CoA reductase (CCR) then reduces cinnamoyl-CoA to using NADPH as a cofactor, advancing the intermediate toward the alcohol form required for in rosavin . Cinnamyl alcohol dehydrogenase (CAD) completes the aglycone synthesis by reducing to , which serves as the direct precursor for rosin upon initial glucosylation. Glycosyltransferases, particularly UDP-glycosyltransferases (UGTs) from the UGT91R subfamily, catalyze the addition of sugar moieties to ; for instance, initial glucosylation forms , while subsequent arabinosylation yields rosavin, with specific enzymes identified through screening for this activity. Native UGTs in remain under characterization, but heterologous systems have demonstrated their role in attaching glucose and . These enzymes are upregulated under abiotic stresses such as cold and heavy metals, enhancing phenylpropanoid flux and rosavin accumulation as a protective response, potentially mediated by transcription factors including MYB family members that regulate pathway genes in plants.

Extraction and Production

Extraction Techniques

Rosavin, a key phenylpropanoid glycoside found primarily in the roots of Rhodiola rosea, is typically isolated through a series of extraction and purification steps from dried plant material. Traditional solvent extraction methods employ ethanol or methanol-water mixtures, often in a 70:30 ratio, at room temperature to selectively dissolve rosavin while minimizing degradation of heat-sensitive compounds. For instance, extraction with 70% ethanol yields approximately 1.2% rosavin from dried roots, outperforming pure water but lower than methanol at 3.3%. These processes usually involve multiple solvent passes to enhance recovery, with yields ranging from 2-4% for total rosavins (including rosavin, rosin, and rosarin) depending on solvent polarity and extraction duration. Maceration and represent foundational techniques for rosavin isolation, where powdered or coarsely ground R. rosea roots are soaked in hydroethanolic solvents (up to 70% ) for 24-48 hours, followed by and concentration under reduced . , a simple soaking method, achieves comparable yields to more advanced approaches, such as 969.71 mg rosavin per 100 g of using 70-75% , and is optimized by agitating the mixture periodically to improve . , involving continuous solvent flow through a packed column of plant material, further refines this by allowing fresher solvent contact, though it requires larger volumes and longer setup times; both methods prioritize room-temperature conditions to preserve bioactivity. Advanced techniques have been developed to improve and . Ultrasound-assisted (UAE) significantly reduces time to as little as 30 minutes while achieving up to 80% , using frequencies of 20-40 kHz to disrupt cell walls and enhance solvent penetration; for example, UAE with natural deep eutectic solvents (NADES) composed of L-lactic acid:: (5:1:11 mol/mol) at 22°C and a 1:40 solid-to-solvent ratio yields 12.23 mg/g rosavin. Supercritical CO₂ , a green alternative, employs CO₂ at 80°C and 5 hours with as a co-solvent, delivering a 4.5% rosavin yield with fewer impurities than conventional solvents, making it suitable for large-scale production. Other innovations, like NADES based on with organic acids, further optimize kinetics under ultrasonic conditions, reaching concentrations of ~1000 μg/mL rosavin after 60 minutes. Purification of crude extracts typically follows extraction to achieve high purity. on or , combined with macroporous resins (e.g., AB-8 type), elevates rosavin content from 3% to over 68% with 85% recovery, while high-speed countercurrent chromatography (HSCCC) isolates rosavin at 97% purity from 13.5 g starting material, yielding 3.4 mg. (HPLC) serves for final polishing, standardizing extracts to at least 3% total rosavins for commercial use, often monitored by UV detection at 205 nm. Overall yields of rosavin extraction are influenced by factors such as root quality, harvest season, and preprocessing (e.g., 0.5-3 mm), averaging 1-3% from dry roots across methods; optimal results stem from 3-5 year-old plants grown in high-altitude conditions. These techniques ensure the isolation of rosavin while maintaining its structural integrity for downstream applications.

Synthetic Methods

The synthesis of rosavin, a diglycoside, has been achieved through various laboratory approaches, primarily focusing on chemical and enzymatic methods to construct its β-D-glucopyranosyl-(1→6)-α-L-arabinopyranoside structure. Early chemical syntheses emphasized multi-step strategies to attach the unit selectively. In a method, the process began with the of 1,2,3,4-diisopropylidene-D-glucopyranose using 2,3,4-tri-O-acetyl-β-L-arabinopyranosylbromide in the presence of silver and under ultrasonic irradiation in anhydrous acetone, yielding the protected in 18%. This intermediate underwent deprotection with acetic acid, with and , and conversion to a thioglycoside activating group using and in , affording the donor in 64% yield. Subsequent coupling with , catalyzed by iodine in , produced the acetylated rosavin in 30% yield, followed by deacetylation with sodium in to isolate rosavin in 95% yield, resulting in an overall process spanning four steps. More recent chemical routes have optimized yields and conditions for scalability. A 2021 seven-step synthesis starting from and fully acetyl-α-D-bromoglucose employed selective protection, , and deprotection sequences under mild conditions, achieving an overall yield of 15.92% with low-cost reagents and minimal contamination. Similarly, another 2021 approach utilized β-D-pentaacetylglucose and 1-hydroxy-2,3,4-triacetylarabinose, involving selective deacetylation with hydrate, promoted by , protection of the glucose 6-OH with tert-butyldiphenylsilyl chloride, and subsequent deprotections with , culminating in rosavin isolation in 75.7% yield over the final two steps of a nine-step sequence noted for its high overall efficiency, operational simplicity, and safety. These multi-step chemical methods typically yield 15-30% overall, relying on Lewis acid activators like BF₃·Et₂O for selective β-glycosidic bond formation, though challenges persist in avoiding anomeric mixtures during assembly. Enzymatic synthesis offers improved and , particularly through cascades using glycosyltransferases. A 2023 one-pot method employed UGT73C5 from to glucosylate to using UDP-glucose as donor, coupled with AtSUS1 sucrose synthase for in situ UDP-glucose regeneration from and , achieving 96% conversion at 2 mM substrate and scaling to 91% at 50 mM with whole-cell biocatalysts. Sequential addition of CaUGT3 from extended the chain with to form rosavin B, an analog, in 62% yield, highlighting the enzymes' for the 1→6 linkage without protection groups. This approach demonstrates higher β- compared to chemical methods, with reaction times reduced to hours under ambient conditions. Semi-synthetic strategies modify related phenylpropanoids, such as sequential of ( monoglucoside) using protected donors and deprotection, though detailed yields remain limited in reported schemes. Recent advances, including 2023 publications on optimized , incorporate assistance to accelerate activation steps, shortening reaction times from days to hours while maintaining yields around 20-30% in total syntheses from onward. Key challenges across methods include ensuring the β-glycosidic linkage at the anomeric position without epimerization, often addressed via thioglycoside or enzymatic donors. A 2024 biosynthetic approach engineered an strain to produce rosavin from D-glucose and L-arabinose, incorporating enzymes such as cinnamate:CoA ligase from Hypericum calycinum, cinnamoyl-CoA reductase from , UDP-glycosyltransferase Bs-YjiC from , and SlUGT91R1 from Solanum lycopersicum, along with UDP-arabinose pathway components. This method achieved titers of 1203.7 ± 32.1 mg/L in shake-flask fermentation and 7539.1 ± 228.7 mg/L in 5-L fed-batch fermentation, providing a sustainable alternative for large-scale production.

Pharmacology

Biological Activities

Rosavin exhibits notable antioxidant properties, primarily through its ability to scavenge free radicals and mitigate oxidative stress. In cell-free assays, it demonstrates potent DPPH radical scavenging activity with an IC₅₀ value of approximately 5.9 μg/mL, indicating strong antioxidant capacity comparable to reference compounds like ascorbic acid. In cellular models, rosavin protects against oxidative damage by inhibiting reactive oxygen species such as superoxide anion (O₂⁻), hydrogen peroxide (H₂O₂), and hypochlorous acid (HOCl), while also scavenging hydroxyl radicals. In vivo, administration of rosavin at doses of 10-50 mg/kg reverses D-galactose-induced oxidative stress in rat models of memory decline, elevating levels of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) while reducing malondialdehyde (MDA). The compound also displays anti-inflammatory effects by suppressing key inflammatory mediators and pathways. Rosavin inhibits the NF-κB signaling pathway and reduces production of pro-inflammatory cytokines such as TNF-α and IL-1β in LPS-stimulated models, including macrophages and lung tissue. In vivo studies using radiation-induced intestinal injury in rats show dose-dependent reductions in TNF-α and IL-1β levels at 20-60 mg/kg, alongside increased anti-inflammatory IL-10. These effects contribute to amelioration of inflammation in hepatic models of non-alcoholic steatohepatitis (NASH), where rosavin at 10-30 mg/kg lowers inflammatory markers without altering underlying pathways in detail. As an , rosavin enhances resistance in animal models of fatigue. Oral administration at 360 mg/kg prolongs time in mice, increases hepatic and glycogen stores, and decreases blood lactate and levels, indicating improved physical resilience. It modulates the hypothalamic-pituitary-adrenal () axis to support , though specific mechanisms are briefly noted in broader studies involving rosavin. Additional biological activities include lipid-lowering effects, where rosavin at 20-40 mg/kg reduces serum triglycerides (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) while elevating high-density lipoprotein cholesterol (HDL-C) in hyperlipidemic mouse and rat models. Analgesic properties are evident in pain assays, with rosavin alleviating oxaliplatin-induced cold allodynia in mice via 5-HT₁A receptor activation at a dose of 10 mg/kg intraperitoneally, showing opioid-like pain relief without direct opioid receptor involvement. In anti-radiation contexts, it protects intestinal epithelial cells (IEC-6) from γ-irradiation, increasing cell viability to 90% at 25 μM post-10 Gy exposure and improving survival rates in irradiated rats at 20-60 mg/kg by reducing epithelial damage. Antitumor activity involves inducing apoptosis and inhibiting proliferation in cancer cell lines, such as small-cell lung cancer cells, where rosavin at 10-100 μM inactivates the MAPK/ERK pathway in vitro. Immunomodulatory effects include boosting natural killer (NK) cell activity and enhancing B lymphocyte proliferation and T cell transformation, with an IC₅₀ of 68 μM observed in Jurkat T cells. These activities are supported by dose-dependent responses in studies from 2018 to 2024, typically at 10-100 mg/kg in vivo.

Mechanisms of Action

Rosavin exerts its antioxidant effects primarily through upregulation of the Nrf2 signaling pathway, which enhances the expression of antioxidant enzymes such as (SOD) and (GSH-Px), thereby mitigating in various tissues. Additionally, as a phenylpropanoid containing hydroxyl groups, rosavin demonstrates direct free radical scavenging activity by quenching through hydrogen atom donation from its moieties. These mechanisms collectively reduce markers of , such as malondialdehyde (MDA), in models of and environmental toxin exposure. In stress adaptation, rosavin contributes to energy homeostasis by activating AMP-activated protein kinase (AMPK) and sirtuin 1 (SIRT1) pathways, which promote mitochondrial biogenesis and cellular resilience under metabolic stress, as observed in models of insulin resistance and high-fat diet-induced damage. The actions of rosavin involve blockade of the /extracellular signal-regulated kinase (MAPK/ERK) signaling cascade, which suppresses downstream inflammatory mediators in lipopolysaccharide-challenged macrophages and lung epithelial cells. Furthermore, rosavin reduces (COX-2) expression in inflamed tissues, leading to decreased production of pro-inflammatory prostaglandins and cytokines such as tumor necrosis factor-alpha (TNF-α). Regarding radioprotection, rosavin enhances DNA repair processes and stabilizes mitochondrial membrane potential, preventing apoptosis and oxidative damage in irradiated tissues through preservation of electron transport chain integrity. In metabolic modulation, rosavin inhibits 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase, the rate-limiting enzyme in cholesterol biosynthesis, thereby lowering serum lipid levels in hyperlipidemic models. Concurrently, it activates peroxisome proliferator-activated receptor gamma (PPARγ), facilitating lipid metabolism and adipocyte differentiation while reducing hepatic steatosis. Rosavin interacts with neurotransmitter receptors through partial agonism at serotonin 5-HT1A receptors, mediating analgesic effects in neuropathic pain models without direct involvement of gamma-aminobutyric acid (GABA) or opioid receptor binding sites.

Applications

Traditional and Adaptogenic Uses

Rhodiola rosea, the primary source of rosavin, has been utilized in Siberian folk medicine for centuries to combat fatigue, enhance vitality, and alleviate symptoms of altitude sickness. In traditional Chinese medicine, extracts containing rosavin have been employed to replenish qi (vital energy) and support the functions of the heart and lung channels. These uses are documented in pharmacopeias dating back to the 1700s, including the first edition of the Swedish Pharmacopoeia in 1755 and Carl Linnaeus's Materia Medica from 1749, which recommended the root for various restorative purposes. In the mid-20th century, Rhodiola rosea was classified as an adaptogen in Russian medicine, a category denoting substances that promote resilience to physical and mental stress without causing sedation or overstimulation. This recognition stemmed from Soviet research in the 1960s, which established the adaptogenic properties of Rhodiola extracts through extensive studies on stress response and endurance. The plant's adaptogenic status has since been incorporated into the Russian Pharmacopoeia, affirming its traditional role in enhancing overall resilience. Standardization to at least 3% rosavin content in extracts emerged from later pharmacological research in the post-1980s era to ensure quality and consistent adaptogenic activity. Culturally, extracts have been employed by Russian cosmonauts during space missions to support endurance and mental acuity under extreme conditions. In Tibetan traditions, monks have used the herb to foster mental clarity and focus during practices, often consuming it as a to aid concentration in high-altitude environments. These applications highlight its historical integration into demanding lifestyles across Eurasian cultures. In contemporary non-clinical contexts, supplements standardized for rosavin are popularly incorporated into regimens to prevent and support cognitive enhancement, reflecting a revival of its traditional adaptogenic heritage. These uses emphasize its role in promoting sustained mental and physical performance without reliance on clinical interventions.

Clinical Applications

Rosavin, a key cinnamyl alcohol glycoside in Rhodiola rosea extracts, has been investigated in human clinical trials primarily through standardized extracts containing 3% rosavins, where it contributes to adaptogenic effects; direct studies on isolated rosavin remain limited to preclinical models. Randomized controlled trials (RCTs) using 200-400 mg/day of such extracts for 4-12 weeks have shown mixed results in reducing burnout scores and symptoms of prolonged or chronic fatigue among stressed adults. For instance, an open-label multicenter trial involving 100 participants with chronic fatigue symptoms found that 400 mg/day of R. rosea extract (standardized to rosavins) over 8 weeks significantly improved fatigue indices. A systematic review of 11 studies (10 RCTs) found contradictory evidence for efficacy in alleviating mental and physical fatigue, with methodological flaws and high risk of bias in many trials. In the context of , rosavin-containing R. rosea extracts have shown promise as an adjunct to selective serotonin reuptake inhibitors (SSRIs). A 2020 randomized, double-blind, placebo-controlled trial evaluated 300 mg/day of R. rosea extract alongside sertraline in patients with mild-to-moderate , reporting significant reductions in Hamilton Depression Rating Scale (HAM-D) scores over 6-12 weeks, indicating improved symptoms without increased adverse events. This adjunctive approach enhanced overall response, with effect sizes comparable to higher SSRI doses alone. Preclinical data suggest potential cardiovascular benefits for rosavin-rich R. rosea extracts, such as regulation in animal models of , though human clinical evidence remains limited. Cognitive enhancement represents another area of clinical interest, with rosavin-standardized extracts improving mental performance in stressed adults. An open-label pilot study from 2020 with 50 participants showed that 200 mg/day enhanced reaction times and during cognitive tasks under . These findings align with broader evidence from RCTs where R. rosea reduced mental and boosted accuracy in prolonged tasks among healthy but stressed volunteers. Emerging potentials include preliminary trials for anxiety and athletic recovery, alongside ongoing research into radioprotection for cancer therapy. An open-label study of 340 mg/day R. rosea extract over 10 weeks in 10 adults with reported significant symptom reductions on standardized scales. For athletic recovery, RCTs indicate 200-600 mg/day improves post-exercise fatigue and markers in trained individuals. A 2025 systematic review supports potential benefits of R. rosea supplementation for endurance exercise performance. Preclinical data on rosavin's radioprotective effects against intestinal injury in models have prompted early-phase human investigations in settings, though clinical trials remain limited. Clinical dosing typically equates to 5-10 mg of pure rosavin through extracts standardized to 3% rosavins, administered as 200-400 mg/day in divided doses for 4-12 weeks to achieve therapeutic effects without exceeding safe limits.

Safety and Toxicology

Adverse Effects

Rosavin, a key in extracts standardized to 3% rosavin content, exhibits a safety profile inferred from the parent extract due to limited isolated compound data. Common side effects of extracts include mild , dry mouth, and , particularly at high doses exceeding 600 mg/day. Rare gastrointestinal upset, such as , has also been noted in some users. In short-term clinical trials, Rhodiola rosea extracts containing rosavin have been generally well-tolerated for up to 10-12 weeks at doses of 200-600 mg/day, with no serious adverse events reported across multiple studies involving hundreds of participants. Toxicity assessments of Rhodiola rosea extracts demonstrate low acute risk, with oral LD₅₀ values exceeding 2000 mg/kg in rodents (e.g., 3360 mg/kg in rats); no dedicated LD₅₀ studies exist for isolated rosavin, but its low acute toxicity is inferred from these extract data. No genotoxic or mutagenic effects have been observed in standard assays. Long-term safety data for rosavin-containing extracts remain limited, with most human studies confined to 12 weeks or less and no evidence of cumulative harm; however, chronic use may increase the risk of overstimulation, manifesting as persistent or . Studies available through 2024 report no hepatotoxicity or nephrotoxicity associated with extracts. In vulnerable populations, extracts should be avoided by individuals with due to documented risks of precipitating . Caution is recommended during , as insufficient data exist on fetal .

Interactions and Contraindications

Rosavin, a key phenylpropanoid in , may interact with various medications due to its adaptogenic and properties. It can exhibit additive serotonergic effects when combined with selective serotonin reuptake inhibitors (SSRIs) such as sertraline or inhibitors (MAOIs), potentially increasing the risk of . Additionally, rosavin-containing extracts may enhance the hypotensive effects of antihypertensive agents like losartan or beta-blockers, necessitating caution in patients managing blood pressure. Through weak inhibition of cytochrome P450 2C9 (CYP2C9), rosavin could alter the metabolism of substrates such as warfarin or ibuprofen, potentially leading to increased drug levels and adverse effects. Other interactions include synergistic central nervous system depression with benzodiazepines, which may amplify . Adaptogenic influences on hormonal regulation warrant caution with thyroid medications like , as rosavin may interfere with function and hormone levels. Contraindications for rosavin include avoidance during and due to insufficient safety data. It is also not recommended for individuals with uncontrolled , as it may exacerbate fluctuations. No major interactions with foods have been reported. Monitoring is advised when using rosavin with antidepressants, including potential dose adjustments to mitigate risks.