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Seroconversion

Seroconversion is the development of detectable antibodies in the blood serum against a specific pathogen, marking the transition from seronegative to seropositive status as a result of natural infection or vaccination.^[1] This process reflects the adaptive immune system's activation, where B cells produce immunoglobulins (primarily IgM initially, followed by IgG) in response to antigens from the infectious agent or vaccine.^[2] The timeline for seroconversion varies by pathogen and host factors but generally occurs within 1 to 6 weeks after exposure for many viral infections, though it can extend to several months in cases like HIV.^[2] Detection is typically confirmed through serological assays, such as enzyme-linked immunosorbent assays (ELISA), which measure antibody titers in paired serum samples taken before and after the expected response period.^[2] Factors influencing the rate and robustness include the individual's immune competence, the pathogen's characteristics, and the type of immune stimulus (e.g., live versus inactivated vaccines).^[3] Seroconversion holds critical significance in clinical and public health contexts, serving as a marker for recent infection, immune protection, and vaccine immunogenicity.^[4] In diagnosis, it helps identify acute or resolved infections during the "window period" when direct pathogen detection may be unreliable, particularly for viruses like HIV or hepatitis.^[5] For vaccination, high seroconversion rates—often defined as a fourfold rise in antibody titers or positivity above a threshold—indicate efficacy and guide booster strategies, with rates exceeding 90% considered protective for many routine immunizations.^[4] Monitoring seroconversion also informs epidemiology, such as estimating infection prevalence or assessing outbreak responses.^[2]

Fundamentals

Definition

Seroconversion is the transition from a seronegative state, characterized by the absence of detectable specific antibodies in the blood serum, to a seropositive state, where antibodies against a particular antigen become measurable due to an immune response triggered by infection or immunization.^[1]^[6] This process reflects the adaptive immune system's activation, primarily involving B cells producing immunoglobulins in response to the antigen.^[1] The detection of seroconversion relies on serological assays that identify antibodies in serum samples, such as enzyme-linked immunosorbent assay (ELISA) or indirect immunofluorescence assay (IFA). These methods typically measure early-appearing IgM antibodies, which indicate recent immune activation, followed by IgG antibodies that persist longer and signify established immunity.^[7]^[8] Importantly, seroconversion marks the point of detectable immune activation rather than the initial onset of infection, as there is often a delay—typically weeks—between pathogen exposure and antibody production sufficient for detection.^[5] For instance, seroconversion can occur in response to viral antigens, such as those from enveloped viruses, or bacterial antigens, like polysaccharides in vaccine formulations, leading to measurable antibody levels without specifying the pathogen's identity.^[1]

Historical Background

The recognition of seroconversion as a key immunological phenomenon originated in the early 20th century amid efforts to diagnose infectious diseases through serological methods. In 1906, August von Wassermann and colleagues introduced the Wassermann test, the first complement-fixation assay for detecting antibodies in the serum of patients with syphilis, thereby establishing the principle of monitoring antibody appearance in response to infection. This test revolutionized diagnostics by identifying immune responses via serum changes, laying the groundwork for understanding seroconversion as the transition to detectable antibody levels, although the specific term emerged later.^[9]^[10] By the mid-20th century, the concept gained prominence in vaccine development, particularly during the 1954 Francis Field Trial of Jonas Salk's inactivated polio vaccine, where antibody production rates served as a primary measure of efficacy. The trial, involving over 1.8 million children, demonstrated that the vaccine induced protective antibody responses in 80-90% of recipients, with pre- and post-vaccination serum testing revealing conversions from seronegative to seropositive states that correlated with reduced paralytic polio incidence. This application highlighted seroconversion rates as a quantifiable endpoint for assessing immunization success, influencing subsequent vaccine evaluation standards.^[11]^[12] A major milestone occurred in the 1980s with the HIV/AIDS epidemic, where seroconversion was formalized as a critical diagnostic marker for recent infection. The licensing of the first enzyme-linked immunosorbent assay (ELISA) for anti-HIV antibodies in 1985 enabled widespread screening of blood products and individuals, detecting seroconversion typically 3-12 weeks post-exposure and preventing thousands of transmissions through blood supply safety measures. This era solidified seroconversion's role in epidemiology and clinical monitoring, as studies linked it to acute retroviral syndrome symptoms and disease progression timelines.^[13]^[14] In the 2000s, advancements shifted toward earlier detection beyond traditional antibody assays, incorporating rapid point-of-care tests and polymerase chain reaction (PCR) for viral nucleic acids. Rapid HIV tests, approved by the FDA starting in 2002, provided results in 20 minutes with sensitivities approaching 99%, facilitating immediate seroconversion assessment in resource-limited settings. Meanwhile, PCR integration reduced the diagnostic window period to 10-33 days post-exposure by identifying viremia before antibody development, enhancing early intervention and outbreak control.^[15]^[16]

Biological Mechanisms

Antibody Production Process

Seroconversion occurs through the humoral immune response, where B lymphocytes produce pathogen-specific antibodies in response to antigen exposure. The process begins with antigen presentation by antigen-presenting cells (APCs), such as dendritic cells or macrophages, which engulf and process foreign antigens, displaying peptide fragments on their surface via major histocompatibility complex class II (MHC II) molecules. These APCs migrate to secondary lymphoid organs, where they present the antigens to naive CD4+ helper T cells, activating them upon recognition by the T cell receptor (TCR). Activated helper T cells then proliferate and differentiate into follicular helper T (Tfh) cells, which provide essential co-stimulatory signals for B cell activation.^[17] B cell activation follows antigen encounter, where naive B cells in lymphoid follicles bind soluble or cell-bound antigens via their B cell receptors (BCRs), typically surface IgM or IgD. This binding leads to antigen internalization, processing, and presentation on MHC II to cognate Tfh cells, resulting in a conjugate pair that exchanges signals: CD40 ligand on T cells binds CD40 on B cells, while cytokines like IL-4 and IL-21 promote B cell proliferation and survival. Activated B cells undergo clonal expansion and differentiate into short-lived plasmablasts or enter germinal centers for further maturation; ultimately, many differentiate into long-lived plasma cells in the bone marrow that secrete large quantities of antibodies, marking the onset of detectable seropositivity.^[17] The initial antibody response is dominated by IgM, produced rapidly by early plasmablasts within days of antigen exposure, providing a pentameric structure for effective early pathogen agglutination and complement activation during primary infection. This is followed by immunoglobulin class-switch recombination (CSR), induced by Tfh-derived cytokines and CD40 signaling, which enables B cells to switch from IgM to other isotypes like IgG, conferring lasting immunity through enhanced opsonization, neutralization, and Fc receptor-mediated functions; IgG typically predominates after 1-2 weeks. In the context of humoral immunity, germinal centers play a pivotal role in affinity maturation: activated B cells proliferate in the germinal center dark zone, undergoing somatic hypermutation via activation-induced cytidine deaminase (AID) to introduce point mutations in BCR variable regions, then compete for antigen on follicular dendritic cells in the light zone, where higher-affinity B cells receive survival signals from Tfh cells, leading to selection of superior clones for antibody diversification and potency.^[17]^[18] This sequence culminates in seroconversion, but a window period exists—the time lag between infection and detectable antibody levels in serum—typically 1-3 weeks for most viral infections, during which molecular diagnostics like PCR may be necessary as antibodies are below assay thresholds.^[19]

Factors Influencing Timing and Rate

Host factors play a significant role in determining the timing and rate of seroconversion, with age being a primary influencer. In older individuals, the immune response is often diminished due to immunosenescence, leading to slower antibody production and lower peak titers following antigenic exposure or vaccination. For instance, seroconversion rates after influenza vaccination decline with age, typically ranging from 40% to 70% in adults aged 60 years and older.^[20] Similarly, immunocompromised hosts, such as those with HIV or undergoing immunosuppressive therapy, experience delayed seroconversion, with antibody detection potentially taking weeks longer than in immunocompetent individuals due to impaired B-cell function and reduced antigen presentation. Genetic variations, particularly in human leukocyte antigen (HLA) genes, also modulate seroconversion; certain HLA class I haplotypes, like those associated with HLA-B*57, are linked to slower HIV disease progression after seroconversion by influencing T-cell mediated control of viral replication.^[21]^[22]^[23] Pathogen-related characteristics further affect seroconversion dynamics. Higher initial viral loads during acute infection can accelerate the onset of detectable antibodies by providing a stronger antigenic stimulus, as observed in SARS-CoV-2 cases where prolonged viremia correlated with higher seroconversion probability and earlier antibody peaks. Conversely, pathogens with high antigenicity variability, such as highly mutable viruses like HIV or influenza, can delay effective seroconversion through immune escape mechanisms, where antigenic drift or shift reduces the efficiency of B-cell recognition and affinity maturation. This evasion prolongs the time to sustained antibody levels, as mutant variants outpace the adaptive immune response.^[24]^[25] External factors, including the route of exposure, co-infections, and nutritional status, can variably impact seroconversion efficiency. Intravenous or percutaneous exposure typically results in faster and higher seroconversion rates compared to mucosal routes, due to greater antigen delivery and systemic dissemination. Co-infections, such as hepatitis C virus alongside HIV, may delay seroconversion by diverting immune resources and inducing chronic inflammation that suppresses B-cell activation. Poor nutritional status, particularly malnutrition, impairs seroconversion by weakening mucosal barriers and reducing overall immune competence, leading to lower antibody responses in undernourished populations.^[26]^[27]^[28] Quantitative measures highlight the variability in seroconversion outcomes across contexts. For many viral vaccines, such as those for hepatitis B or measles, seroconversion rates reach 95% or higher within 4-6 weeks post-immunization in healthy adults, reflecting robust primary responses. Antibody half-lives post-seroconversion vary by isotype, with IgG typically persisting for 21-28 days in circulation due to FcRn-mediated recycling, though long-term stability can extend to decades for certain antiviral antibodies, influencing the duration of detectable immunity.^[29]^[30]

Terminology and Variations

Core Terms

Seropositive describes an individual whose blood serum contains detectable levels of specific antibodies against a pathogen, signifying prior or ongoing exposure to that infectious agent.^[31] This status is confirmed through serological testing and is a key indicator in diagnosing infections such as HIV or hepatitis. Seronegative indicates the absence of detectable antibodies to a specific pathogen in the serum, typically implying no prior exposure or infection, though it may occur during the early window period before antibodies develop.^[31] In clinical contexts, seronegative results guide initial assessments but require follow-up testing to rule out recent infections.^[32] Seroprevalence measures the proportion of individuals in a population who test seropositive for antibodies against a particular pathogen, providing an epidemiological estimate of cumulative exposure or immunity.^[33] It is calculated as the number of seropositive persons divided by the total tested, often used to track disease burden in communities.^[34] Seroconversion rate quantifies the percentage of previously seronegative individuals who become seropositive within a specified timeframe after exposure or vaccination, reflecting the efficiency of immune response onset.^[35] This metric is essential for evaluating infection transmission dynamics and vaccine efficacy in population studies.^[36] Seroreversion refers to the loss of detectable specific antibodies in the serum over time, resulting in a negative serological test following a prior positive result.^[37] This phenomenon can occur in resolved infections where the pathogen is cleared and antibody levels naturally decline below assay detection thresholds, or in cases of waning immunity after vaccination or initial exposure.^[38] For instance, in situations of transient immunity, such as post-vaccination antibody decay, seroreversion highlights the temporary nature of humoral protection and the potential need for booster doses to maintain detectable antibody levels.^[38] Primary seroconversion occurs during an individual's first exposure to a specific antigen, characterized by a slower onset of antibody production dominated initially by immunoglobulin M (IgM), followed by a rise in immunoglobulin G (IgG).^[39] This initial response involves naive B cells and typically takes several days to weeks to reach detectable levels, reflecting the time required for immune system activation and clonal expansion.^[40] In contrast, a secondary immune response, triggered by re-exposure to the same or a closely related antigen, elicits a rapid and robust recall response primarily through IgG antibodies, mediated by memory B cells, leading to higher peak titers and shorter latency periods.^[39] This distinction underscores the role of immunological memory in enhancing protection against recurrent infections.^[40] Asymptomatic seroconversion describes the development of detectable antibodies in response to infection without the manifestation of clinical symptoms, often observed in subclinical or mild cases.^[23] This process indicates that the adaptive immune system can mount an effective humoral response even in the absence of overt disease, particularly in chronic or low-virulence infections where viral replication occurs at levels insufficient to cause noticeable illness.^[23] Such events contribute to the spread of pathogens through undetected carriers while still conferring partial immunity via antibody production.^[41] Cross-reactivity arises when antibodies generated against one antigen bind to structurally similar epitopes on unrelated or related antigens, potentially leading to misinterpretation of serological tests during seroconversion assessment.^[42] This phenomenon occurs due to shared molecular motifs that mimic the original immunizing epitope, allowing polyclonal antibodies to recognize multiple targets with varying affinity.^[42] In diagnostic contexts, cross-reactivity can complicate the specificity of assays, as prior exposures to similar pathogens may produce false-positive results for a new infection, necessitating confirmatory testing with orthogonal methods.

Clinical Applications

Diagnostic and Monitoring Role

Seroconversion plays a central role in serological diagnostics for detecting immune responses to natural infections, primarily through antibody-based assays that identify the transition from seronegative to seropositive status. Common testing methods include enzyme-linked immunosorbent assay (ELISA), which measures specific antibody levels such as IgM and IgG, often defining seroconversion as a four-fold or greater rise in titers.^[2] Western blot serves as a confirmatory test following initial ELISA positivity, providing specificity by detecting multiple viral antigens to reduce false positives.^[43] For acute infections, polymerase chain reaction (PCR) complements serology by detecting viral nucleic acids during the pre-seroconversion window, though it does not directly assess antibody production.^[44] In clinical applications, seroconversion testing enables screening for prior exposure in asymptomatic individuals, such as in population seroprevalence studies, and supports blood donor safety by identifying recent infections that could transmit pathogens like HIV or hepatitis B.^[45] It also aids in staging disease progression, for instance, by confirming resolved acute phases in viral illnesses through rising antibody levels. Rapid antibody tests, often lateral flow assays, facilitate point-of-care screening but require laboratory confirmation due to lower specificity.^[46] Interpretation of seroconversion results presents challenges, including false positives from cross-reactivity with related pathogens and false negatives during early or delayed immune responses. To address this, paired sera—collected during acute and convalescent phases (typically 2-4 weeks apart)—are recommended to demonstrate dynamic titer changes, enhancing diagnostic accuracy over single-sample testing.^[47] The window period before detectable antibodies further complicates timing, necessitating integrated approaches with molecular tests.^[48] For monitoring, serial serological testing tracks seroconversion in at-risk populations, such as healthcare workers or those following potential exposures, with repeat assessments at 4-6 weeks and up to 12 weeks post-exposure to confirm status after prophylaxis. This approach is particularly vital in post-exposure management for HIV, where ongoing surveillance detects breakthrough infections despite preventive measures.^[43]^[49]

Role in Vaccination

Seroconversion serves as a key metric for assessing vaccine efficacy in clinical trials, often defined as a four-fold increase in antibody titers or achievement of a protective threshold post-vaccination, and is frequently used as a primary endpoint to evaluate immunogenicity.^[4] For instance, the measles-mumps-rubella (MMR) vaccine typically achieves seroconversion rates exceeding 90% after two doses, indicating robust induction of protective antibodies against measles.^[50] This measure correlates with clinical protection, allowing regulators to approve vaccines based on demonstrated immune responses rather than solely infection outcomes.^[51] Booster doses play a critical role in eliciting secondary seroconversion, rapidly elevating and sustaining antibody titers that wane over time after primary vaccination. In studies of COVID-19 vaccines, a third dose has induced seroconversion in over 50% of individuals who were seronegative following the initial series, thereby prolonging humoral immunity.^[52] Similarly, for rabies vaccines, boosters maintain adequate antibody levels even years after primary immunization, ensuring long-term protection.^[53] These secondary responses are essential for vaccines where initial titers decline, such as those against tetanus or influenza, to prevent breakthrough infections.^[54] Population-level seroconversion contributes to herd immunity by achieving seroprevalence thresholds that interrupt transmission chains. For measles, a vaccination coverage leading to at least 95% seropositivity is required to establish community protection, as lower levels allow outbreaks in susceptible individuals.^[55] This threshold reflects the pathogen's basic reproduction number, where widespread seroconversion from vaccination reduces the effective reproduction rate below one.^[56] Challenges in achieving high seroconversion rates persist for certain vaccines and populations, necessitating strategies like adjuvants to enhance immune responses. The hepatitis B vaccine, for example, yields lower seroconversion rates—around 26% in hemodialysis patients compared to over 96% in healthy controls—due to impaired immunity in chronic kidney disease.^[57] Adjuvants, such as alum or novel formulations, address these gaps by amplifying antigen presentation and T-cell activation, improving rates in low-responders like the elderly or immunocompromised.^[58] Factors like age can further influence outcomes, with older adults often showing reduced seroconversion compared to younger groups.^[59]

Seroconversion in Specific Infections

In HIV

Seroconversion in HIV infection typically occurs 2 to 8 weeks after initial exposure, during which anti-HIV antibodies become detectable in standard serological tests.^[60] This period often coincides with acute retroviral syndrome, affecting 40% to 90% of individuals and presenting with flu-like symptoms such as fever, rash, lymphadenopathy, and fatigue, which arise due to high viral replication before immune control is established. The median onset of these symptoms precedes seroconversion by about 18 days, marking the acute phase of infection.^[5] The diagnostic window period before seroconversion poses challenges for antibody-based testing, as infected individuals remain seronegative but highly infectious. To bridge this gap, p24 antigen assays detect the viral capsid protein as early as 14 to 20 days post-exposure, while nucleic acid tests (NAT) for HIV RNA can identify infection 10 to 33 days after exposure, enabling earlier diagnosis.^[60]^[43] These tests are crucial for high-risk scenarios, reducing the window period and facilitating prompt intervention.^[61] Seroconversion signifies the transition from acute to chronic HIV infection, where detectable antibodies correlate with partial viral control but ongoing replication. Clinical outcomes vary: rapid progressors, defined as those with CD4 counts dropping to ≤100 cells/mm³ within the first year post-seroconversion, experience accelerated disease advancement and comprise about 2.8% of cases.^[62] In contrast, elite controllers—rare individuals (0.1% to 2.5% of infections) who maintain undetectable viral loads (<50 copies/mL) without therapy—demonstrate robust innate immune responses, often linked to specific HLA alleles like B*57.^[63] These phenotypes highlight genetic and immunological factors influencing progression.^[64] Management emphasizes early antiretroviral therapy (ART) initiation immediately following seroconversion or diagnosis to preserve CD4 counts, suppress viral load, and mitigate transmission risk. ART started within 3 months of infection enhances immunological recovery and limits reservoir establishment compared to delayed treatment.^[65] Elite controllers represent an exception, as they often forgo ART due to sustained viral suppression, though monitoring for complications like inflammation is advised.^[66]

In Hepatitis B

Seroconversion in hepatitis B virus (HBV) infection refers to the immunological shift from detectable viral antigens to protective antibodies, marking resolution of acute infection or, less commonly, clearance in chronic cases.^[67] The primary markers include the loss of hepatitis B surface antigen (HBsAg) and the emergence of antibody to HBsAg (anti-HBs), which together indicate recovery and immunity from past infection.^[68] Antibody to hepatitis B core antigen (anti-HBc) persists lifelong and serves as a marker of prior exposure, distinguishing natural infection from vaccine-induced immunity where anti-HBc is absent.^[67] In acute HBV infection, seroconversion follows an early phase characterized by the appearance of HBsAg within 1-12 weeks of exposure, accompanied by IgM anti-HBc, which emerges shortly after and confirms ongoing acute infection.^[68] Resolution occurs in approximately 95% of immunocompetent adults, with HBsAg clearing and anti-HBs developing, often within 4-6 months, leading to lifelong immunity.^[69] In contrast, chronic infection develops in fewer than 5% of acutely infected adults, featuring persistent HBsAg beyond 6 months without IgM anti-HBc, and full seroconversion to anti-HBs remains delayed or incomplete in most cases, occurring spontaneously in less than 1% annually.^[69]^[67] Following HBV vaccination, seroconversion is assessed by anti-HBs levels, with concentrations ≥10 mIU/mL after the complete three-dose series indicating protective immunity in about 95% of healthy individuals.^[68] This threshold correlates with long-term protection against infection, though antibody titers may wane over time without loss of cellular immunity.^[67] A complication of HBV seroconversion is occult HBV infection, defined as the presence of replication-competent HBV DNA in the liver (and sometimes serum) despite HBsAg negativity, often in seronegative individuals lacking detectable anti-HBc or anti-HBs.^[70] This state, potentially arising from immune suppression of viral replication or low-dose primary infection, carries risks of reactivation, particularly under immunosuppression, and may contribute to hepatocellular carcinoma or transmission.^[70]

In COVID-19

Seroconversion in SARS-CoV-2 infection typically occurs within 1-3 weeks after symptom onset, with IgM and IgG antibodies against the spike protein emerging simultaneously or in close succession.^[71] Studies have shown that IgM levels typically peak around 15-22 days post-symptom onset, followed by a decline, while IgG persists longer and provides the basis for serological detection.^[72] This timeline reflects the acute nature of COVID-19 as a respiratory virus, where antibody responses align with viral clearance.^[73] With the emergence of variants like Omicron, seroconversion dynamics have shifted toward faster onset but weaker antibody titers compared to earlier strains such as the ancestral virus. Primary infection with Omicron elicits a reduced neutralizing antibody response, limiting cross-protection against pre-Omicron variants.^[74] This attenuated response underscores the variant's immune evasion properties, though overall seroconversion rates remain high in infected individuals.^[75] Post-2020 research highlights the role of hybrid immunity—combining prior infection and vaccination—in enhancing seroconversion outcomes, achieving rates up to 95-97% in exposed populations across multiple waves.^[76] This synergistic effect results in higher and more sustained antibody levels than infection or vaccination alone. In diagnostic contexts, serosurveys have leveraged these responses to estimate COVID-19 prevalence, revealing waning antibody titers by 6-12 months post-infection in many cases, which complicates long-term immunity assessments.^[77]^[78] By 2025, investigations into long COVID have linked persistent symptoms to low-level seropositivity, with some patients maintaining detectable antibodies beyond 12 months despite overall waning.^[79] Booster vaccinations continue to bolster seroconversion, significantly reducing reinfection risk by elevating neutralizing antibodies and extending protection against variants.^[80]^[81]

In Other Viral Infections

Seroconversion in hepatitis C virus (HCV) infection typically occurs between 4 and 10 weeks after exposure, with anti-HCV antibodies detectable in >97% of individuals by 6 months after exposure.^[82] Spontaneous viral clearance, which may accompany seroconversion in acute cases, occurs in about 25% of infections, usually within the first 6 months, leaving the majority to progress to chronicity.^[83] In influenza infections, seroconversion develops rapidly, with initial antibody responses detectable within 7 to 14 days post-infection and peak titers often reached by 4 to 7 weeks.^[84] These responses are generally short-lived, with hemagglutination inhibition titers declining over several months to a year, contributing to the rationale for annual vaccination strategies to maintain protective immunity.^[85] Seroconversion following Zika virus (ZIKV) or dengue virus (DENV) infection elicits cross-reactive antibodies that persist long-term but can facilitate antibody-dependent enhancement (ADE) during heterologous infections.^[86] For instance, primary ZIKV infection boosts DENV-cross-reactive titers, increasing the risk of severe dengue in subsequent exposures by enhancing viral entry into immune cells.^[86] For emerging viruses like mpox (formerly monkeypox), seroconversion during natural infection since the 2022 global outbreak shows variability, particularly in immunocompromised patients, where delayed or attenuated antibody responses have been associated with prolonged disease and complications.^[87]

References

[1]
Seroconversion (Concept Id: C4042908) - NCBI
Seroconversion refers to the first development of antibodies to an infective agent, as a result of infection or immunisation.
[2]
Seroconversion - an overview | ScienceDirect Topics
Seroconversion (the presence of antibodies against HIV in a previously nonreactive individual) usually occurs within 6 months of exposure to HIV. ▫. Detection ...Missing: timeline | Show results with:timeline
[3]
The role of serological testing for vaccination with a focus on ...
Sep 17, 2025 · Seroconversion after vaccination against measles and rubella is typically robust (even in people who became mild-moderately immunocompromised ...
[4]
Vaccine strain affects seroconversion after influenza ... - Nature
Jan 24, 2022 · Seroconversion was defined as fourfold increase in antibody HI titre above 1:40 post-influenza vaccination, also known to be associated with a ...
[5]
Identifying the Early Post-HIV Antibody Seroconversion Period - PMC
As there is not a specific length of time since seroconversion that can be clearly defined as the most important duration to measure, we considered 3 time ...Missing: definition | Show results with:definition
[6]
Serodynamics: A primer and synthetic review of methods for ... - NIH
Seroconversion, The change in an individual's immune status from seronegative to seropositive (e.g., due to an immune exposure). This term can also be used at ...
[7]
Longitudinal Profile of Immunoglobulin G (IgG), IgM, and IgA ...
The median times of seroconversion for IgG, IgM, and IgA detected by the indirect immunofluorescence assay were 17 days (17 days by ELISA), 16.5 days (20.5 days ...
[8]
Serology characteristics of SARS-CoV-2 infection since exposure ...
May 19, 2020 · The cumulative seroconversion curve showed that the rate for Ab and IgM reached 100% and IgG reached 97.1% on days 16, 21 and 29 post symptom ...
[9]
A Brief History of Laboratory Diagnostics for Syphilis
Jan 6, 2020 · Despite its pitfalls, the Wassermann test was one of the first serological tests for the diagnosis of infectious diseases and the basis for ...
[10]
The 100th anniversary of Wassermann-Neisser-Bruck reaction
Carl Bruck (1879-1944), German dermatologist and venereologist, developed the first serologic test for the diagnosis of syphilis.
[11]
1955 Polio Vaccine Trial Announcement
The Salk vaccine has been proved to be up to 80-90 percent effective in preventing paralytic polio.
[12]
“A calculated risk”: the Salk polio vaccine field trials of 1954 - NIH
The results, announced in 1955, showed good statistical evidence that Jonas Salk's killed virus preparation was 80-90% effective in preventing paralytic ...
[13]
Human Immunodeficiency Virus Diagnostic Testing: 30 Years ... - NIH
Mar 2, 2016 · The first HIV antibody test, developed in 1985, was designed to screen blood products, not to diagnose AIDS. The first-generation assays ...
[14]
How One Test Changed HIV | Abbott Newsroom
Much of that changed on March 1985, when the FDA licensed the first test to screen blood for exposure to HIV, developed by Abbott. The approval marked a ...
[15]
The Evolution of HIV Diagnostic Testing - News-Medical
Nov 29, 2024 · Innovations in HIV diagnostics, including rapid tests and nucleic acid amplification, are critical for timely detection and effective ...
[16]
The Evolution of HIV Testing - Artron Laboratories Inc
Aug 5, 2024 · Second and third-generation HIV tests were developed in the late 1980s. They were more accurate and faster than the previous generation tests.
[17]
The Humoral Immune Response - Immunobiology - NCBI Bookshelf
The activation of B cells and their differentiation into antibody-secreting plasma cells (Fig. 9.1) is triggered by antigen and usually requires helper T cells.Missing: presentation | Show results with:presentation
[18]
Germinal Centers - Annual Reviews
Apr 26, 2022 · Germinal centers (GCs) are microanatomical sites of B cell clonal expansion and antibody affinity maturation. Therein, B cells undergo the ...
[19]
Flow-Cytometry Intracellular Detection and Quantification of HIV1 ...
Aug 4, 2022 · 2 In general, the “window period”, the time from the initial ... seroconversion is consistently 1–3 weeks.9 Therefore, rapid antibody ...
[20]
Should we vaccinate the better seroconverters or the most ... - NIH
Dec 2, 2021 · The antibody response tends to be slower in older people, and significantly lower peak titers are reached after vaccination in elderly ...
[21]
Factors that predict severity of infection and seroconversion in ...
Aug 2, 2022 · Preliminary studies in non-immunocompromised subjects with COVID-19 infection reported seroconversion 7 to 14 days following symptom onset, with ...
[22]
Influence of HLA Class I Haplotypes on HIV-1 Seroconversion and ...
We examined the effect of HLA class I haplotypes on HIV-1 seroconversion and disease progression in the Pumwani sex worker cohort.
[23]
Viral Load Influences Seroconversion to SARS-CoV-2 in Those at ...
Jan 9, 2024 · A longer detectable viral load duration was associated with an increased chance of seroconversion postinfection. The mean duration of detectable ...
[24]
Antigenic escape is accelerated by the presence of ... - NIH
Nov 9, 2022 · Accumulating evidence indicates that each VOC evades host immunity developed by previous infections with pre-existing strains and vaccines [6,7] ...
[25]
Postexposure HIV Prophylaxis in Physicians and Medical Personnel
Mar 3, 2025 · Route of Exposure. Risk with HIV-Positive Source. Factors Increasing Risk ; Percutaneous. ≈ 1 in 300 (0.3%). Hollow-bore needles, visibly bloody ...
[26]
A Tale of Two Viruses: Immunological Insights Into HCV/HIV ...
In this review, we will discuss the interplay between HIV and HCV during coinfection. Specifically, how HIV influences HCV infectious outcome and liver disease ...
[27]
A scoping review: the impact of nutritional status on the efficacy ...
Jul 15, 2025 · Poor seroconversion in people with obesity has been reported [94], raising questions about whether this is due to a failure to boost immunity, ...
[28]
Should we vaccinate the better seroconverters or the most ...
Dec 2, 2021 · In young persons, seroconversion is seen in 80–90% within 2 weeks of the first dose, and 95–100% of vaccinees have had seroconversion by 4 weeks ...
[29]
Duration of Humoral Immunity to Common Viral and Vaccine Antigens
Nov 8, 2007 · Antiviral antibody responses were remarkably stable, with half-lives ranging from an estimated 50 years for varicella–zoster virus to more than 200 years for ...<|control11|><|separator|>
[30]
[PDF] Glossary of HIV/AIDS-Related Terms
For example, HIV seropositive means that a person has detectable antibodies to HIV; seronegative means that a person does not have detectable HIV antibodies.
[31]
Definition of seronegative - NCI Dictionary of Cancer Terms
A seronegative test result usually means that a person has not been exposed to or infected with a virus or other infectious agent and has not made antibodies ...
[32]
[PDF] Surveillance and Seroepidemiology - CDC Stacks
Prevalence from serosurveys, or seroprevalence, is defined as the number of persons whose sera contain a particular bio- marker among the total number of ...
[33]
Human Serology & Flu | Influenza (Flu) - CDC
Sep 17, 2024 · A seroprevalence survey is an investigation that uses serology tests (also known as antibody tests) to estimate the percentage of people in a ...
[34]
Pre-Omicron seroprevalence, seroconversion, and seroreversion of ...
Seroconversion rates were defined as the number of newly seropositive participants divided by the sum of participants' time under observation while at risk of ...
[35]
[PDF] Clinical Data Needed to Support the Licensure of Seasonal ... - FDA
2) rates of seroconversion, defined as the percentage of subjects with either a pre-vaccination HI titer < 1:10 and a post-vaccination HI titer > 1:40 or a ...
[36]
Absence of seroreversion in 80 HAART-treated HIV-1 seropositive ...
Feb 16, 2006 · Findings. Seroreversion, defined as a quantitative decrease in specific antibody levels so that they measure below the cutoff of an assay, can ...
[37]
Decline in SARS-CoV-2 Antibodies After Mild Infection Among... - CDC
Nov 27, 2020 · A limited number of studies have found declines in SARS-CoV-2 antibody levels over time (7–9), but the frequency and timing of seroreversion ( ...
[38]
Acquired Immunity - Immune Disorders - Merck Manuals
The response triggered by the first encounter with an antigen is the primary immune response. IgM then attaches to the antigen, activating the complement system ...Lymphocytes · T Cells · Antibodies
[39]
Immunoglobulin - StatPearls - NCBI Bookshelf - NIH
IgG is synthesized mostly in the secondary immune response to pathogens. IgG can activate the classical pathway of the complement system, and it also is ...Immunoglobulin · Function · Clinical Significance
[40]
The immunology of asymptomatic SARS-CoV-2 infection - Nature
Oct 19, 2021 · It is clear that adaptive immunity is strongly activated during asymptomatic infection, but some features of the T cell and antibody response may differ from ...
[41]
Specificity and Cross-Reactivity - NCBI - NIH
Cross-reactivity measures the extent to which different antigens appear similar to the immune system.Antibody Paratopes · Natural Antibodies—Low... · Cross-Reaction of Polyclonal...
[42]
Seroconversion Is Misleading as a Test for HSV-2 Infection in ... - NIH
May 29, 2025 · Seroconversion is defined as a four-fold or greater rise in antibody titers. This assay is used in human prophylactic vaccine trials to confirm ...
[43]
HIV Testing - StatPearls - NCBI Bookshelf - NIH
Sep 21, 2025 · ... (ELISA) technology, performed as rapid point-of-care diagnostics. Positive results must be confirmed with a serum-based Western blot. A key ...Hiv Testing · Diagnostic Tests · Interfering Factors<|control11|><|separator|>
[44]
Diagnostic Performance of Three ELISAs for Detection of Antibodies ...
Aug 16, 2022 · Seroconversion for SARS-CoV-2 is estimated to occur 7–14 days after the onset of symptoms, when the sensitivity of the PCR decreases, making the ...
[45]
Leveraging donor populations to study the epidemiology ...
Broadly, the study contributed to blood donation, diagnostic and public health screening, and later development of effective therapeutics that are being ...
[46]
Diagnostic Accuracy Assessment of Immunochromatographic Tests ...
Acute scrub typhus was defined as 1) ≥ 4-fold increase in IFA IgM titer, 2) seroconversion, 3) a high static titer of ≥ 1:25,600 between acute and convalescent ...Missing: challenges | Show results with:challenges<|control11|><|separator|>
[47]
Linking multiple serological assays to infer dengue virus infections ...
Similarly, paired sera (e.g. sera obtained at acute and convalescent time-points) are also usually evaluated using a cut off metric to identify seroconversions ...
[48]
Serological assays for emerging coronaviruses: Challenges and ...
No differentiation between acute and convalescent sera. Specificity of serological assays is vital to avoid false positive diagnoses. Critically, some of ...
[49]
PEP to Prevent HIV Infection - NCBI Bookshelf - NIH
Factors that increase the risk of transmission: Many factors that contribute to HIV infection are shared by the 4 post-exposure prophylaxis (PEP) scenarios ...
[50]
Low IgG Seroconversion among Persons Vaccinated against ... - CDC
Jan 1, 2025 · The overall seroconversion rate was 38.7% (p<0.0001). Among patients who received 1 dose of vaccine, only 57 (38%) seroconverted (p<0.0001), and ...
[51]
Clinical Endpoints for Evaluating Efficacy in COVID-19 Vaccine Trials
Oct 22, 2020 · We propose a general set of clinical endpoints to facilitate a harmonized evaluation and comparison of the efficacy of vaccine candidates, overall and across ...
[52]
Study of efficacy and longevity of immune response to third ... - NIH
Results: We demonstrate that a third dose of COVID-19 vaccine leads to seroconversion in 57% of patients that were seronegative after primary vaccination series ...
[53]
Rabies Antibody Response After Booster Immunization
Although studies showed that antibody levels decline over time, adequate booster responses were still retained over long time intervals.
[54]
Safety and Immunogenicity of Single-Dose of Adsorbed Tetanus ...
In the Phase III clinical trial, the primary endpoint was the seroconversion rate of tetanus antibodies at 30 days post-vaccination. The secondary endpoints ...<|control11|><|separator|>
[55]
How Herd Immunity Works | History of Vaccines
Feb 22, 2025 · Governments use herd immunity thresholds to set vaccination goals. For example: Measles: 95% vaccination rate. Polio: 80% vaccination rate.Missing: seroprevalence seroconversion
[56]
Is Herd Immunity Against SARS-CoV-2 a Silver Lining? - Frontiers
Assuming an R0 estimate of 5.7, using the mathematical formula 1-1/R0, the herd immunity threshold for COVID-19 would be ~82.5%, meaning that the incidence of ...
[57]
What is the hepatitis B vaccination regimen in chronic kidney disease?
Jan 1, 2018 · The authors reported seroprotection rates of 26.1% in patients on hemodialysis, 55.2% in patients with stage 3 or 4 CKD, and 96.2% in controls.
[58]
Vaccine Adjuvants: Putting Innate Immunity to Work - PMC
... vaccines, even those using alum adjuvant, do not achieve adequate seroconversion rates or protective antibody titers. Moreover, responses to vaccines begin ...
[59]
Insights into vaccines for elderly individuals: from the impacts of ...
Apr 10, 2024 · Clinical studies have indicated that intradermally injecting influenza vaccine can improve the titer and seroconversion rates in elderly ...
[60]
Getting Tested for HIV - CDC
The window period depends on the type of HIV test. Antibody tests can usually detect HIV 23 to 90 days after exposure. Most rapid tests and self-tests are ...
[61]
p24 revisited: A landscape review of antigen detection for early HIV ...
Here we review the landscape of p24-diagnostics to identify knowledge gaps and barriers and help shape future research agendas.
[62]
Evaluation of Rapid Progressors in HIV Infection as an Extreme ...
Rapid HIV disease progression is an extreme HIV phenotype, although there is little consensus on a definition. Differences in HIV disease progression can be ...
[63]
Elite Control of HIV Infection: Implications for Vaccine Design - NIH
“Elite controllers” are rare HIV-infected individuals who are able to spontaneously control HIV replication without medication.
[64]
A high prevalence of potential HIV elite controllers identified over 30 ...
Mar 2, 2021 · Elite controllers comprise approximately 0.1-2.5% of all HIV infections worldwide and are less likely to develop AIDS [16]. These patients ...
[65]
Enhanced Immunological Recovery With Early Start of Antiretroviral ...
Here we confirmed that early ART, defined as ART introduced within 3 months from AEHI, has a beneficial effect on immune function in a large setting of patients ...
[66]
Clinical outcomes and antiretroviral therapy in 'elite' controllers
Elite controllers naturally suppress HIV viraemia below the level of detection using standard methods, but demonstrate persistent inflammation and low-level ...
[67]
Screening and Testing for Hepatitis B Virus Infection - CDC
Mar 10, 2023 · The three main serologic markers used to determine HBV infection status are hepatitis B surface antigen (HBsAg), antibody to hepatitis B surface ...
[68]
Chapter 10: Hepatitis B | Pink Book - CDC
May 9, 2024 · Serologic markers for HBV infection include HBsAg, antibody to HBsAg (anti-HBs) ... They should be screened first for HBsAg, anti-HBc and anti-HBs.
[69]
Hepatitis B: Diagnosis and Treatment - AAFP
Apr 15, 2010 · Fewer than 5 percent of adults acutely infected with hepatitis B virus progress to chronic infection. The diagnosis of hepatitis B virus ...
[70]
Occult Hepatitis B Virus Infection: An Update - PMC - NIH
Seronegative OBI refers to OBI individuals negative for all HBV serum markers including anti-HBc and anti-HBs, in whom the only detectable marker of HBV ...
[71]
Comparison of SARS-CoV-2 IgM and IgG seroconversion profiles ...
To track seroconversion of IgG and IgM antibodies in patients with SARS-CoV-2 infection and its association with patient and clinical factors and outcomes.
[72]
Available COVID-19 serial seroconversion panel for validation of ...
IgM levels peaked on day 50 (prior to IgG) and declined in subsequent samples. This seroconversion panel is a useful tool for validation of SARS-CoV-2 antibody ...
[73]
Antibody seroconversion in asymptomatic and symptomatic patients ...
Seroconversion was defined as IgG ≥ 10 AU mL−1 or IgM ≥ 10 AU mL−1. During hospitalisation, serum samples were collected at different time points when ...Missing: definition | Show results with:definition
[74]
Monitoring of antibodies against SARS-CoV-2 over 2 years ... - Nature
Jul 9, 2025 · Prior studies reported that primary Omicron infection elicits a poor neutralizing antibody response, and the cross-reactivity to pre-Omicron ...
[75]
Impact of Omicron Variant Infection on Assessment of Spike-Specific ...
Mar 8, 2023 · The currently prevailing variants of SARS-CoV-2 are subvariants of the Omicron variant. The aim of this study was to analyze the effect of ...
[76]
Natural and hybrid immunity following four COVID-19 waves
Despite little disease, most participants were seropositive with rates rising from 52% to 74%, 90% and 97% after waves 1, 2, 3 and 4 respectively. Antibodies to ...
[77]
Waning of anti-SARS-CoV-2 antibodies after the first wave of ... - NIH
These results from the period before COVID-19 vaccination show that in the general population, antibody titers decline in one-third of individuals from 6 months ...
[78]
Waning Immune Humoral Response to BNT162b2 Covid-19 ...
Oct 6, 2021 · Six months after receipt of the second dose of the BNT162b2 vaccine, humoral response was substantially decreased, especially among men, among persons 65 years ...Missing: serosurveys prevalence
[79]
Humoral anti-SARS-CoV-2 response in patients with different long ...
Long COVID (LC) is characterized by persistent symptoms following SARS-CoV-2 infection, with various mechanisms offered to explain its pathogenesis.
[80]
Longitudinal antibody titers measured after COVID-19 mRNA ...
Sep 17, 2025 · Previous research has also reported that additional doses (booster vaccinations) can rapidly induce high antibody titers (12), even in groups ...
[81]
New data underlines impact of COVID-19 booster vaccines
Oct 23, 2025 · A major new study suggests COVID-19 booster vaccines offer significant protection even in people who've had vaccines or previous COVID-19 ...Missing: seroconversion | Show results with:seroconversion
[82]
Viral Hepatitis - STI Treatment Guidelines - CDC
Jul 22, 2021 · The average time from exposure to antibody to HCV (anti-HCV) seroconversion is 4−10 weeks, and anti-HCV can be detected among approximately 97% ...
[83]
Like a Rolling Stone? A Review on Spontaneous Clearance of ...
Aug 30, 2024 · estimated the average chance of HCV clearance following acute infection at about 25% [18]. In general, prospective studies consistently report ...
[84]
Individual and Population Trajectories of Influenza Antibody Titers ...
... seroconversion peaks—that is, 5–8 weeks after infection. The time window of detectable seroconversion estimated for H1N1 influenza and the time window of ...
[85]
Persistence of Antibodies to Influenza Hemagglutinin and ... - NIH
HAI and NAI titers decreased slowly over 18 months; overall, a 2-fold decrease in antibody titer was estimated to take >600 days for all HA and NA targets.Serologic Laboratory Assays · Results · Figure 2<|separator|>
[86]
Dengue and Zika virus infection in children elicit cross-reactive ... - NIH
Dengue and Zika virus infection in children elicit cross-reactive protective and enhancing antibodies that persist long term.
[87]
Severe mpox in an immunocompromised patient complicated by ...
Oct 4, 2024 · We report prolonged mpox (>14 weeks) in a patient with HIV complicated by deep tissue MPXV infection despite two courses of tecovirimat treatment.