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Stachyose

Stachyose is a non-reducing tetrasaccharide belonging to the raffinose family of oligosaccharides (RFOs), consisting of two α-D-galactose units linked to sucrose via α(1→6) glycosidic bonds, with the full structure being α-D-galactopyranosyl-(1→6)-α-D-galactopyranosyl-(1→6)-α-D-glucopyranosyl-(1→2)-β-D-fructofuranoside.^[1] Its molecular formula is C₂₄H₄₂O₂₁, and it exhibits high solubility in water along with thermal stability, making it suitable for various biochemical applications.^[1]^[2] Naturally occurring in numerous plant species, stachyose is particularly abundant in legumes such as soybeans, beans, and peas, where it constitutes a major component of seed storage carbohydrates, typically comprising 4-6% of dry weight in soybean seeds.^[3]^[4]^[5] It is also present in cucurbit fruits, Japanese artichoke rhizomes, and other vegetables, serving as a key oligosaccharide alongside raffinose and verbascose.^[6]^[7] In plants, stachyose plays a critical role in carbon storage, desiccation tolerance during seed maturation, and enhancing seed vigor and longevity by acting as an osmoprotectant against abiotic stresses like drought and cold.^[8]^[9] Biosynthesis occurs via sequential galactosylation of sucrose, with stachyose synthase catalyzing the addition of a second galactose unit to raffinose using galactinol as the donor.^[10]^[11]^[12] In human health contexts, stachyose is indigestible by mammalian enzymes and reaches the colon intact, where it functions as a prebiotic by selectively promoting the growth of beneficial gut microbiota such as Bifidobacterium species, potentially aiding in modulation of the microbiome and reducing inflammation.^[13]^[14] However, its fermentation by colonic bacteria can produce gas, leading to flatulence and bloating, which classifies it as an antinutritional factor in high-legume diets unless processed to reduce RFO content.^[15]^[4] Emerging research highlights its potential therapeutic uses, including hepatoprotective effects against liver injury and applications as a sucrose substitute in food due to its low caloric value and stability.^[16]^[2]

Chemical Structure and Properties

Molecular Structure

Stachyose is a tetrasaccharide composed of two α-D-galactose units, one α-D-glucose unit, and one β-D-fructose unit.^[1] The molecular formula of stachyose is C_{24}H_{42}O_{21}.^[1] These monosaccharide units are connected through specific glycosidic linkages: the structure is α-D-galactopyranosyl-(1→6)-α-D-galactopyranosyl-(1→6)-α-D-glucopyranosyl-(1↔2)-β-D-fructofuranoside, commonly abbreviated as Gal(α1→6)Gal(α1→6)Glc(α1↔2β)Fruf.^[17] This configuration forms a linear chain where the β-D-fructofuranose is linked to the α-D-glucopyranose via a (1↔2) glycosidic bond, characteristic of the sucrose core, and the two α-D-galactopyranose units are sequentially attached via α(1→6) glycosidic bonds to the 6-position of the glucose.^[18] In standard representations, stachyose is depicted as a linear extension of the raffinose trisaccharide, with an additional α-D-galactose unit appended to the terminal galactose via another α(1→6) linkage.^[19] Stachyose is structurally analogous to raffinose, a related trisaccharide that consists of one α-D-galactose unit, one α-D-glucose unit, and one β-D-fructose unit linked as Gal(α1→6)Glc(α1↔2β)Fruf, differing by the absence of the second galactose extension.^[19] This incremental addition of galactose units via α(1→6) bonds defines the raffinose family of oligosaccharides, with stachyose representing the tetrasaccharide member.^[20]

Physical and Chemical Properties

Stachyose is a tetrasaccharide with the molecular formula \ce{C24H42O21} and a molar mass of 666.58 g/mol.^[1] It typically appears as a white to off-white crystalline powder.^[21] The compound exhibits high solubility in water, dissolving at approximately 50 mg/mL to form a clear, colorless solution, while remaining insoluble in ethanol and other organic solvents.^[21] Stachyose possesses a mild sweetness, equivalent to about 22% of sucrose on a weight basis, making it suitable for applications requiring low-sugar profiles.^[22] Its melting point is around 170 °C, and it provides approximately 2 kcal/g (8 kJ/g) through fermentation by colonic microbiota, as it is indigestible by human enzymes in the upper gastrointestinal tract.^[23] As a non-reducing sugar—owing to the fructose unit's participation in the glycosidic linkage—stachyose demonstrates excellent thermal and acid stability, resisting decomposition under high temperatures and acidic conditions.^[22] Complete hydrolysis of stachyose, whether by acid or enzymes such as α-galactosidase and invertase, produces one glucose, one fructose, and two galactose units.^[24]

Biosynthesis and Occurrence

Biosynthesis

Stachyose biosynthesis in plants occurs primarily through the sequential addition of galactose units to sucrose within the raffinose family oligosaccharides (RFOs) pathway. The process begins with the formation of raffinose, a trisaccharide, from sucrose and galactinol catalyzed by raffinose synthase (RS; EC 2.4.1.82). Subsequently, stachyose synthase (STS; EC 2.4.1.67) transfers a galactosyl group from either UDP-galactose or galactinol to raffinose, yielding stachyose and either UDP or myo-inositol, respectively. The primary reaction is galactinol + raffinose → stachyose + myo-inositol.^[25]^[26]^[27] This enzymatic cascade is facilitated by upstream production of galactinol from myo-inositol and UDP-galactose via galactinol synthase (GOLS; EC 2.4.1.123), providing the galactosyl donor for both RS and STS. STS is a monomeric enzyme with a molecular mass of approximately 88-90 kDa, exhibiting specificity for raffinose as the acceptor substrate while demonstrating additional galactosyltransferase activity toward cyclitols like pinitol in some species. Biosynthesis predominantly occurs in sink tissues such as developing seeds, leaves, roots, and tubers of dicotyledonous plants, particularly legumes, where RFOs accumulate to high levels.^[25]^[28]^[27] Regulation of stachyose synthesis is tightly linked to plant development and environmental stress responses, with STS expression and activity induced during seed maturation in legumes like soybean and adzuki bean. Transcript levels of STS genes, such as Glyma19g40550 in soybean, peak midway through seed development (around 20-22 days after flowering), correlating with stachyose accumulation up to approximately 60 µmol g⁻¹ dry mass in mature seeds. This pathway is evolutionarily conserved in RFO-synthesizing plants as a mechanism for desiccation tolerance and stress protection during seed dormancy. In vitro multienzyme systems mimicking this cascade have been developed using extracts from plant sources to synthesize stachyose from sucrose, confirming the sequential nature of the reactions.^[26]^[27]^[25]^[29]

Natural Sources

Stachyose, a tetrasaccharide belonging to the raffinose family of oligosaccharides (RFOs), occurs naturally in a variety of plants, primarily accumulating in seeds, roots, and tubers where it co-occurs with raffinose and verbascose. It serves as a storage carbohydrate and contributes to desiccation tolerance in seeds by maintaining low water activity and providing osmotic protection during maturation and dormancy.^[30] In legumes, stachyose is particularly abundant, often comprising a significant portion of the soluble carbohydrates. Soybean (Glycine max) seeds contain stachyose at concentrations ranging from 1.4% to 4.1% of dry weight (14–41 g/kg), making it the predominant RFO and a key component alongside sucrose. Other pulses, such as chickpeas (Cicer arietinum), exhibit stachyose levels up to 5.9% (59.4 mg/g dry matter), while lentils (Lens culinaris) have 1.6–2.2% (16–22 mg/g), peas (Pisum sativum) 1.5–3.8%, and lupin species (Lupinus spp.) 4.4–8.4% (44–84 mg/g) depending on cultivar.^[30]^[31] Green beans (Phaseolus vulgaris), adzuki beans (Vigna angularis), and faba beans (Vicia faba) also harbor notable amounts, typically 0.8–4.7 mg/g, supporting their role in seed development.^[30] Beyond legumes, stachyose is found in certain roots and tubers, where it accumulates to high levels in specialized species. Chinese artichoke (Stachys sieboldii) tubers are a rich source, with stachyose comprising 23.6% of dry weight (236 mg/g) or 10–15% of fresh weight, positioning it as one of the highest natural concentrations.^[32] Rehmannia (Rehmannia glutinosa) roots contain stachyose as a major oligosaccharide, often exceeding 10% of dry matter in processed extracts, though exact levels vary with growth conditions. In sugar beets (Beta vulgaris), stachyose is present in minor amounts alongside raffinose, typically below 0.1% in roots, contributing to overall carbohydrate diversity. Environmental factors influence stachyose accumulation, with higher levels observed in drought-resistant plants and during seed development under stress, enhancing desiccation tolerance by stabilizing cellular structures. For instance, RFOs like stachyose increase in seeds of tolerant species to buffer against dehydration, a pattern noted across dicot crops.^[30]

Metabolism

In Plants

Stachyose serves as a compatible solute in plants, contributing to osmotic regulation and providing protection against environmental stresses such as desiccation, cold, and drought. As a member of the raffinose family oligosaccharides (RFOs), it accumulates in response to water deficit, helping maintain cellular turgor and stabilize proteins and membranes without disrupting enzymatic activity.^[7] In drought-stressed leaves, genes involved in stachyose synthesis, such as stachyose synthase, are upregulated, enhancing tolerance by acting as an osmolyte.^[33] During cold acclimation in species like Arabidopsis thaliana, stachyose levels increase alongside other RFOs to mitigate freezing damage through similar protective mechanisms.^[34] In seeds, stachyose functions as a major soluble carbohydrate, serving as an energy reserve that supports germination by storing carbon in a stable, non-reducing form. It accumulates during late seed maturation, correlating with the acquisition of desiccation tolerance, which allows orthodox seeds to remain viable in dry conditions for extended periods.^[35] For instance, in soybeans (Glycine max), stachyose buildup during gradual drying of immature embryos enhances seed storability and vigor by suspending metabolism and preventing oxidative damage.^[35] During seed germination, stachyose undergoes catabolism through hydrolysis by α-galactosidase enzymes, releasing galactose, glucose, and fructose for metabolic use. This breakdown mobilizes stored reserves, with α-galactosidase activity peaking as RFO levels decline, facilitating energy provision to the emerging seedling.^[36] In legumes like soybeans, this enzymatic process ensures efficient conversion of stachyose to usable monosaccharides without intermediate toxicity.^[37] Stachyose is translocated via the phloem in certain plant families as part of the RFO pathway, where it is synthesized in the minor veins of leaves and serves as a primary transport sugar. In species such as cucumbers (Cucumis sativus), stachyose synthase activity in companion cells drives its loading into the phloem for distribution to sink tissues like roots and seeds.^[38] This symplastic pathway predominates in RFO-transporting plants, including members of Cucurbitaceae and Fabaceae, enabling efficient long-distance carbon allocation.^[8] In specific examples, stachyose aids seed viability in crops like soybeans and adzuki beans (Vigna angularis), where its accumulation during maturation supports desiccation tolerance and long-term storage.^[35] However, in drought-sensitive plants such as Nicotiana benthamiana, elevated stachyose under stress can trigger apoptotic-like cell death, characterized by DNA fragmentation and nuclear condensation, contrasting with resilient species that regulate it to avoid such outcomes.^[39]

In Humans and Animals

Stachyose, a tetrasaccharide composed of two galactose units linked to sucrose, is not hydrolyzed in the upper gastrointestinal (GI) tract of humans due to the absence of the enzyme α-galactosidase, which is required to cleave its α-1,6-galactosidic bonds. As a result, it passes through the stomach and small intestine intact and reaches the colon, where it serves as a substrate for microbial fermentation.^[40]^[41] In the human colon, stachyose is metabolized by gut microbiota, including species such as Bifidobacterium spp. and Bacteroides thetaiotaomicron, through fermentation processes that yield short-chain fatty acids (SCFAs) like acetate, propionate, and butyrate, as well as gases including hydrogen (H₂), carbon dioxide (CO₂), and methane (CH₄). This fermentation can lead to flatulence and bloating, particularly with higher intakes, as the gases are produced in quantities that exceed normal expulsion capacity. Stachyose exhibits prebiotic potential by selectively supporting beneficial microbiota, though detailed effects on health outcomes are addressed elsewhere.^[42]^[43]04239-3/fulltext) In animals, stachyose digestion varies by species. Monogastric animals, such as pigs and humans, share the limitation of lacking endogenous α-galactosidase, resulting in colonic fermentation similar to that in humans and associated flatulence or reduced nutrient utilization in high-soy diets. In contrast, ruminants like cattle utilize rumen microbiota that produce α-galactosidase, enabling hydrolysis and fermentation of stachyose in the rumen for energy extraction without significant gas-related issues in the hindgut. Some insects, such as termites (Reticulitermes speratus), also digest stachyose via hindgut bacterial enzymes, including α-galactosidase from species like Bacteroides-related microbes, supporting efficient carbohydrate breakdown.^[44]^[45] Direct absorption of intact stachyose is minimal in humans and monogastric animals, with studies showing no significant uptake in small intestinal epithelial cells; although not absorbed, recent research (as of 2024) indicates stachyose binds to membranous HSP90β on small intestinal epithelial cells, regulating exosomal miRNA cargo and influencing gut microbiota composition.^[40] Instead, benefits arise indirectly from microbial byproducts like SCFAs, which are absorbed and contribute to energy metabolism. Regarding safety, stachyose is generally recognized as non-toxic, with no acute toxicity reported in animal models, though excessive intake in monogastrics can cause transient gastrointestinal discomfort such as bloating.^[40]^[46]^[47]

Applications and Health Effects

Industrial Uses

Stachyose is primarily extracted from soybeans and other legumes, such as Stachys species, through water-based methods involving hot water or boiling extraction of dehulled materials to solubilize the oligosaccharide, followed by filtration to remove solids.^[48] Purification typically includes clarification to eliminate impurities, decolorization using activated carbon, ion-exchange chromatography for desalting, and vacuum concentration to prepare for crystallization, yielding a high-purity white powder product.^[49] Additionally, enzymatic synthesis from sucrose using multienzyme systems, such as galactinol synthase and raffinose synthase combined with stachyose synthase, offers a scalable alternative for commercial production, achieving yields up to 656 mg from optimized reactions.^[50] In the food industry, stachyose serves as a low-calorie bulk sweetener with low sweetness (approximately 20-30% that of sucrose, varying by source) and excellent water solubility, making it suitable for incorporation into beverages like soy milk, tea, juices, and malt drinks to enhance texture and provide prebiotic functionality without altering flavor significantly.^[51]^[22] It is also added to baked goods and functional foods, such as fermented dairy products, where it improves stability under heat and contributes to mild sweetness while supporting gut health benefits in formulations.^[52] For animal feed, stachyose is supplemented in legume-based diets at levels around 1% to mitigate anti-nutritional factors by promoting the growth of beneficial gut bacteria, including increased lactobacilli in the ileum and bifidobacteria in the cecum and colon, thereby enhancing overall digestibility and reducing pathogen loads in livestock like pigs and broilers.^[53] Stachyose is incorporated into prebiotic supplements, such as powders and capsules, often at concentrations of 1-5 g per serving, to leverage its role in selectively stimulating bifidobacteria proliferation for digestive support.^[22] In pharmaceuticals, it functions as a stabilizer and excipient in formulations like tablets, capsules, and oral liquids due to its stability and non-digestible nature.^[54] In cosmetics, stachyose is utilized for its moisturizing properties in skincare products, aiding hydration retention and soothing effects through prebiotic action on skin microbiota.^[55]

Physiological Benefits

Stachyose exhibits prebiotic effects by selectively promoting the growth of beneficial gut bacteria, such as Bifidobacterium and Lactobacillus species, while suppressing pathogenic ones like Clostridium perfringens.^[56] In human studies, supplementation with 5 g/day of stachyose-enriched α-galacto-oligosaccharides for 14 days significantly elevated fecal levels of these probiotics in healthy adults, enhancing overall microbiota diversity.^[56] Animal models further demonstrate that stachyose increases the abundance of Akkermansia muciniphila and Bacteroidetes, contributing to a balanced gut ecosystem that supports intestinal barrier integrity through upregulated tight junction proteins like ZO-1 and occludin.^[57] In terms of gastrointestinal health, stachyose alleviates constipation by increasing bowel movement frequency and fecal bulk. A 30-day clinical trial in 103 constipated patients showed that 5 g/day of stachyose improved defecation frequency, stool softness, and ease of passage compared to placebo, with no significant adverse effects reported.^[56] In rodent models of slow transit constipation, stachyose supplementation restored microbiota composition, boosted fecal weight (up to 284.83 mg per pellet versus 105 mg in controls), and shortened transit time, suggesting potential preventive benefits for human functional constipation.^[58] Additionally, stachyose mitigates symptoms of ulcerative colitis in dextran sulfate sodium-induced mouse models by reducing colonic inflammation, lesion severity, and pro-inflammatory cytokines through microbiota modulation and increased bacterial diversity.^[59] Stachyose confers metabolic benefits, particularly in regulating blood glucose and lipid profiles. In spontaneous type 2 diabetic KKAy mice, stachyose enhanced insulin sensitivity, reducing the homeostatic model assessment of insulin resistance (HOMA-IR) index by modulating gut microbiota and short-chain fatty acid production, with greater efficacy when combined with berberine (79.3% reduction versus 61.2% for berberine alone).^[57] It also lowers fasting blood glucose and HbA1c levels while improving glucose tolerance, as evidenced by decreased area under the curve in oral glucose tolerance tests.^[57] In high-fat diet/streptozotocin-induced diabetic rats, oral stachyose administration reduced blood lipids and enhanced insulin sensitivity by targeting sodium-glucose cotransporter 2-mediated glucose reabsorption and curbing renal inflammation.^[60] Beyond these, stachyose reduces systemic inflammation in metabolic disorder models, such as nonalcoholic fatty liver disease, by remodeling bile acid profiles via gut microbiota alterations, thereby attenuating hepatic cholesterol accumulation.^[61] Recent research as of 2024 includes a study in obese children showing that stachyose supplementation alters gut microbiota composition and metabolic profiles, potentially aiding obesity management. Additionally, in vitro and ex vivo studies have revealed that nondigestible stachyose interacts with membranous HSP90β on small intestinal epithelial cells, influencing exosomal miRNA secretion and intestinal homeostasis, providing a novel mechanism for its physiological effects.^[62]^[40] Research highlights include in vitro and in vivo studies showing microbiota shifts toward beneficial taxa, with safe dosages up to 5 g/day in humans demonstrating laxative effects without toxicity; higher doses around 10-15 g/day may amplify benefits but require further validation.^[56]^[63] However, limitations persist, including potential side effects like gas production from microbial fermentation, and while promising, most evidence derives from animal models with limited large-scale human trials needed to confirm long-term efficacy in metabolic syndrome.^[57]

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Stachyose is a tetrasaccharide consisting of sucrose having an alpha-D-galactosyl-(1->6)-alpha-D-galactosyl moiety attached at the 6-position of the glucose.
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Stachyose Attenuates Hepatic Cholesterol Deposition in ... - PubMed
Oct 15, 2025 · Stachyose Attenuates Hepatic Cholesterol Deposition in Nonalcoholic Fatty Liver Mice via Gut Microbiota-Driven Bile Acid Profile Remodeling.
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Stachyose alleviates coronary heart disease by modulating the ...
Aug 25, 2025 · The dosage of SM for rats was determined to be 1.35 g/kg per day, based on the 15 g dosage used in human clinical practice. SMP constitutes ...Missing: trials | Show results with:trials