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Acetate

Acetate is the anion with the CH₃COO⁻, serving as the conjugate base of acetic (CH₃COOH) through the removal of a proton from its carboxy group. It exists as a monocarboxylic anion and forms the basis for a wide class of salts and esters derived from acetic . In chemistry, acetate compounds are ubiquitous, with salts like (CH₃COONa) exhibiting properties such as alkalinizing and effects when used in medical applications. Esters, such as (CH₃COOC₂H₅), function as polar aprotic solvents in and industrial processes, formed by the reaction of acetic with . These compounds are characterized by their relatively low toxicity and versatility, though some, like , require handling precautions due to flammability and irritant potential. Biologically, acetate plays a pivotal role as a key in and cellular processes, acting as the most common building block for pathways, including the production of fatty acids and other . It is generated during the metabolism of carbohydrates, fats, and proteins, and participates in energy production via the after conversion to . In and textiles, refers to , a semisynthetic derived from the of , where at least 92% of hydroxyl groups are acetylated to form the . This material is widely used in the production of fabrics, films, and plastics due to its silk-like sheen, , and ease of , though it has limitations in strength and compared to fully synthetic fibers.

Fundamentals

Definition and Nomenclature

The acetate ion, denoted as CH₃COO⁻ or C₂H₃O₂⁻, is the conjugate derived from acetic acid (CH₃COOH) through of its carboxyl group. This anion plays a central role in numerous chemical and biological processes due to its ability to act as a or . The term "acetate" originates from the Latin word acetum, meaning "vinegar," reflecting the long-standing association of acetic acid with the sour liquid produced by the fermentation of ethanol. Acetate compounds, such as the anciently known salt lead(II) acetate, have been prepared through various techniques since antiquity, with systematic chemical studies of vinegar's components advancing in the 18th century. In , the International Union of Pure and Applied Chemistry (IUPAC) designates the systematic name for the anion as ethanoate, derived from the parent acid's IUPAC name, ethanoic acid, though the retained "acetate" is preferred and universally accepted for general use. Salts of the anion follow the pattern of naming the cation followed by "acetate" (or "ethanoate"), such as for NaCH₃COO. Esters are named as alkyl acetate (or alkyl ethanoate), exemplified by (CH₃COOC₂H₅) for the compound formed from acetic acid and ethanol. The general formulas for acetate-derived compounds are straightforward: salts are expressed as M(CH₃COO)n, where M is a metal or cationic and n corresponds to its , while esters take the form CH₃COOR, with R representing an alkyl or .

Molecular Structure and Properties

The acetate ion (CH₃COO⁻) features a characterized by delocalization of the negative charge across the two oxygen atoms in the group, resulting in two equivalent resonance forms. This delocalization leads to identical C-O lengths of approximately 1.25 for both bonds, intermediate between typical (1.36 ) and (1.20 ) C-O bonds. The of the acetate ion is planar overall, with the moiety exhibiting trigonal planar coordination around the central carbonyl carbon atom, featuring bond angles close to 120°. This structure arises from sp² hybridization of the carbon atom and allows for effective π-overlap in the system. Physically, the acetate ion demonstrates high in owing to its polar, ionic character, enabling strong interactions with molecules. As the conjugate base of acetic acid (pKₐ = 4.76 at 25°C), it behaves as a with pK_b ≈ 9.24, partially hydrolyzing in to generate ions and establish a mildly basic . Chemically, the acetate ion readily acts as a acceptor via its oxygen lone pairs, facilitating and interactions in polar media. It undergoes to reform acetic acid or (CH₃COO⁻ + H₂O ⇌ CH₃COOH + OH⁻), contributing to its utility in buffering systems. In salts, O-H stretching from is observed in spectra around 3400 cm⁻¹, while the carboxylate group displays asymmetric stretching at ~1550 cm⁻¹ and symmetric stretching at ~1410 cm⁻¹, confirming the resonance-stabilized .

Acetate Salts

Preparation and General Properties

Acetate salts are primarily synthesized through the neutralization of acetic acid with the corresponding or . For instance, is produced by reacting acetic acid with , yielding the and water:
\ce{CH3COOH + NaOH -> CH3COONa + H2O}
This method is widely used industrially and in laboratory settings due to its simplicity and high yield. Alternatively, acetate salts can be prepared via metathesis reactions, involving the exchange of ions between two compounds, such as reacting a soluble acetate with another to form the desired product, often followed by or .
As ionic compounds, acetate salts consist of metal cations paired with the acetate anion (\ce{CH3COO^-}). Alkali metal acetates, like those of sodium and potassium, are highly soluble in water, with solubilities often exceeding 100 g/100 mL at room temperature. Many exhibit hygroscopicity, absorbing atmospheric moisture to form hydrates. These salts generally demonstrate good thermal stability; for example, sodium acetate melts at 324°C and remains stable up to decomposition temperatures around 500°C or higher for some alkali variants. In aqueous solutions, acetate salts undergo , in which the acetate ion acts as a :
\ce{CH3COO^- + H2O ⇌ CH3COOH + OH^-}
This results in mildly basic solutions, with values typically above 7 for moderate concentrations. The acetate ion serves as the conjugate base of acetic acid (pKa ≈ 4.76), allowing these salts to form effective acetate buffers when mixed with acetic acid, maintaining near 4.76.
Acetate salts often crystallize in both and hydrated forms, depending on preparation conditions and . , for example, commonly forms the trihydrate (\ce{CH3COONa \cdot 3H2O}), which adopts a monoclinic and is stable under ambient conditions, while the form is obtained by and has an orthorhombic .

Common Salts and Applications

(CH₃COONa) is a widely utilized salt known for its efflorescent nature, whereby the trihydrate form loses upon exposure to air, forming the compound. This property makes it suitable for applications requiring controlled hydration states. In consumer products, trihydrate is employed in reusable heating pads and hand warmers, where allows it to remain liquid until triggered to crystallize, releasing heat through an . As a designated E262, it functions as a , acidity regulator, and flavor enhancer in products like baked goods and condiments, and it is by the U.S. when used within specified limits. Additionally, serves as a key component in solutions for maintaining in laboratory and industrial settings, particularly in biochemical assays due to its compatibility with acetic acid. Calcium acetate (Ca(CH₃COO)₂) is primarily applied in medical contexts for managing in patients with end-stage renal disease undergoing . It acts as a by forming insoluble complexes in the , thereby reducing dietary absorption and helping to maintain serum levels below 6.0 mg/dL. This use is supported by clinical evidence showing its efficacy comparable to other binders like , with potentially lower risks of hypercalcemia when dosed appropriately. While it provides supplemental calcium, which can contribute to effects in some formulations, its primary therapeutic role remains phosphate control rather than routine antacid therapy. Lead acetate (Pb(CH₃COO)₂), historically known as "sugar of lead" for its sweet taste, was used as a in ancient wine and preparations, contributing to widespread among elites. Its toxicity, which includes neurotoxic effects leading to symptoms like and , was recognized even in but persisted in use until modern regulations. Today, is strictly restricted; the U.S. banned its use in , including progressive hair dyes, effective January 2022, due to absorption risks and lack of safety data supporting external application. Among other acetate salts, (KCH₃COO) finds application as an environmentally friendlier de-icing agent on runways and roads, where it lowers the freezing point of to -60°C without the corrosive content of traditional salts. (Zn(CH₃COO)₂), meanwhile, is incorporated into throat lozenges to alleviate symptoms, with studies indicating that doses of 13.3 mg can reduce cold duration by up to 2-3 days through antiviral mechanisms, though efficacy varies and side effects like may occur. In , acetate salts such as serve as precipitating agents in qualitative inorganic analysis schemes. For instance, is used to detect sulfide ions by forming insoluble precipitates, aiding in the separation and identification of anions in complex mixtures. This application leverages the selective precipitation behavior of acetate-derived lead ions in buffered solutions.

Acetate Esters

Synthesis and General Properties

Acetate esters are primarily synthesized through the esterification reaction, in which acetic acid reacts reversibly with an in the presence of an acid catalyst such as to form the and :
\ce{CH3COOH + ROH ⇌ CH3COOR + H2O}.
This equilibrium-driven process typically requires removal of to favor ester formation and is widely used for preparing simple acetate esters.
Alternative synthetic routes include , where an acetate ester exchanges its alkoxy group with another , often catalyzed by acids or bases, allowing conversion between without isolating the intermediate. Another method involves the of with an , which proceeds rapidly under mild conditions to yield the acetate ester and HCl, though this requires handling the reactive acid chloride. Acetate esters exhibit with boiling points generally ranging from 50°C to 200°C, depending on the alkyl chain length of the R group, making lower homologs useful as solvents. They often possess characteristic fruity odors due to their molecular , contributing to their sensory profiles. varies with : short-chain acetate esters are miscible with , while longer-chain ones are less soluble and more compatible with nonpolar solvents. Hydrolysis of acetate esters reverses their formation, yielding acetic acid and the original ; acid-catalyzed equilibrates similarly to esterification, whereas base-catalyzed , known as , is irreversible and produces the acetate . The rate of depends on conditions like , , and the ester's structure, with basic conditions accelerating the reaction for practical applications. Acetate esters are generally stable under neutral conditions, exhibiting low reactivity that allows their use as solvents in organic synthesis. However, they can undergo oxidation to carboxylic acids or other products under strong oxidizing conditions, and certain unsaturated acetate esters, like vinyl acetate, are prone to polymerization.

Common Esters and Uses

Ethyl acetate is one of the most widely produced acetate esters, serving primarily as a in industries such as paints, coatings, and adhesives, as well as in nail polish removers and as an agent in chemical processing. Global production of ethyl acetate reached approximately 4.5 million metric tons in , reflecting its high demand in solvent applications and flavoring agents. Vinyl acetate, a key in , is synthesized industrially through the reaction of with acetic acid and oxygen over a catalyst, enabling large-scale production for processes. It is predominantly used to produce (PVAc) for adhesives and coatings, and (PVA) for textiles and paper sizing, with global production volumes exceeding 7 million metric tons annually as of 2024. Cellulose acetate, derived from the esterification of with , forms the basis for versatile materials including acetate fibers used in textiles and apparel for their silk-like properties, photographic films for image support, and cigarette filters due to their porous structure that traps . This ester's applications leverage its biodegradability and optical clarity, making it suitable for consumer goods like eyeglass frames and tool handles. Isopropyl acetate functions as an effective cleaning in industrial formulations and serves as a pharmaceutical for the and of active pharmaceutical ingredients (), owing to its low toxicity and compatibility with organic compounds. It is also employed in coatings and inks as a fast-evaporating for resins such as and polyesters. Most common acetate esters, including ethyl, vinyl, and isopropyl variants, are highly flammable liquids with points below 20°C, posing hazards in handling and storage; they require and ignition source avoidance per safety guidelines. profiles generally indicate low acute risks, with primary concerns being eye, , and respiratory upon , though exhibits potential carcinogenicity based on studies in animals.

Biological Role

Acetate in Metabolism

Acetate serves as a key intermediate in cellular metabolism, primarily through its activation to acetyl-coenzyme A (acetyl-CoA), which enables integration into central energy pathways. This activation is catalyzed by the enzyme acetyl-CoA synthetase (ACS), which facilitates the ligation of acetate to coenzyme A using ATP as an energy source, yielding the reaction: \text{CH}_3\text{COO}^- + \text{CoA} + \text{ATP} \rightarrow \text{acetyl-CoA} + \text{AMP} + \text{PP}_i The process involves two steps: first, acetate reacts with ATP to form acetyl-adenylate (acetyl-AMP), followed by nucleophilic attack by coenzyme A to produce acetyl-CoA. This activation is essential across prokaryotes and eukaryotes, allowing acetate to participate in catabolic and anabolic processes. Once formed, from acetate enters the tricarboxylic acid () cycle, serving as the primary entry point for two-carbon units derived from the oxidation of carbohydrates, fats, and other substrates. In the cycle, condenses with oxaloacetate in a reaction catalyzed by to form citrate, which undergoes subsequent transformations to produce , NADH, FADH₂, and GTP (or ATP). These reducing equivalents feed into the , driving and ATP synthesis, thus linking acetate metabolism to overall cellular energy production. Under aerobic conditions, this pathway efficiently oxidizes acetate-derived carbons, though partial flux into the cycle can also support biosynthetic needs like . In humans, dietary acetate from ingested sources like is rapidly absorbed in the upper , while acetate produced by microbial in the gut from fermented products and is predominantly absorbed in the colon via passive diffusion and monocarboxylate transporters, entering the for delivery to the liver. Hepatic rapidly converts this acetate to via ACS, utilizing it for energy production, , or synthesis, with excess potentially contributing to body formation during or low-carbohydrate states. This process helps regulate , as acetate can suppress and modulate glucose utilization. Disorders involving acetate accumulation, such as those in chronic alcoholism, arise from ethanol's sequential oxidation to and then acetate by and , respectively, overwhelming normal clearance and leading to elevated circulating levels. This excess acetate contributes to , particularly in , where it exacerbates acid-base imbalance alongside ketone accumulation from fat oxidation. Symptoms include and , often requiring supportive to restore acid-base equilibrium. From an evolutionary perspective, acetate's role as an energy source traces back to ancient anaerobic microbes, where it supported ATP generation via the acetyl-CoA cleavage pathway or substrate-level phosphorylation in primordial environments lacking oxygen. In archaea and early bacteria, acetate metabolism facilitated carbon fixation and energy conservation in anoxic conditions, underscoring its foundational importance in the development of core metabolic networks. This conserved mechanism persists in modern anaerobes, highlighting acetate's enduring significance in microbial physiology.

Fermentation Involving Acetate

Acetogenic fermentation represents a key microbial process in environments where certain , known as acetogens, produce acetate from one-carbon compounds such as and . These obligately , including aceticum, utilize the Wood-Ljungdahl pathway as their primary mechanism for energy conservation and autotrophic growth, converting CO₂ and H₂ into acetate via the reductive pathway. The core reaction in this process is \ce{CO2 + 4H2 -> CH3COO^- + H^+ + 2H2O}, facilitated by enzymes such as formyltetrahydrofolate synthetase, methenyltetrahydrofolate cyclohydrolase, and methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase, which are highly conserved across acetogenic species. This pathway not only supports microbial growth in hydrogen-rich habitats like sediments and guts but also contributes to global carbon cycling by fixing CO₂ into . In contrast, acetate is produced as a byproduct during by yeasts such as , where the primary pathway decarboxylates pyruvate to , followed by reduction to via . However, a parallel route generates acetate through the oxidation of by NADP⁺-dependent (encoded by ALD6), which helps balance cofactors under conditions but reduces overall yield. This side production typically accounts for minor acetate accumulation in industrial fermentations, influencing product quality and requiring to minimize it for higher efficiency. Acetate also serves as a substrate for utilization in methanogenic processes, particularly by acetotrophic methanogens like Methanosarcina species, which cleave acetate into methane and carbon dioxide through the aceticlastic pathway. In this reaction, \ce{CH3COO^- + H^+ -> CH4 + CO2}, the methyl group of acetate is reduced to CH₄ while the carbonyl group is oxidized to CO₂, enabling these archaea to derive energy in anaerobic ecosystems. Methanosarcina acetivorans, for instance, performs this cleavage efficiently in marine sediments and bioreactors, contributing significantly to biogenic methane production. Industrially, acetate is generated via the of by such as species, a process central to production. These oxidize to acetic acid through membrane-bound and , yielding vinegars with 4–20% acetic acid content depending on the substrate and conditions. This oxidative fermentation occurs in submerged or surface methods, with Acetobacter pasteurianus being a common strain that tolerates high acid concentrations, ensuring efficient conversion in large-scale operations. Environmentally, acetate plays a pivotal role in systems for production, acting as the primary intermediate and precursor for from organic waste. In these consortia, acetate-oxidizing syntrophs and acetoclastic methanogens convert acetate to and CO₂, accounting for up to 70% of in stable digesters treating food waste or . Disruptions like acetate accumulation can inhibit methanogens, but its balanced turnover enhances recovery from .

Coordination Chemistry and Applications

Acetate as a

Acetate (CH₃COO⁻) serves as a versatile in coordination , primarily binding to metal centers through its two oxygen atoms, which are equivalent due to . This ambidentate nature allows acetate to adopt various coordination modes, including monodentate (η¹-O), where only one oxygen atom coordinates to a single metal, bidentate chelating (κ²-O,O), with both oxygens binding to the same metal, and bridging configurations such as syn-syn bidentate (μ₂-η¹:η¹), syn-anti bidentate (μ₂-η¹:η²), or asymmetric bridging. These modes influence the geometry and reactivity of metal complexes, often stabilizing polynuclear structures through bridges. A prominent example of syn-syn bridging is found in the copper(II) acetate dimer, [Cu₂(μ-O₂CCH₃)₄], where four acetate ligands bridge two Cu(II) centers in a paddlewheel arrangement, resulting in a short Cu-Cu distance of approximately 2.63 Å and antiferromagnetic coupling between the d⁹ ions. This structure exemplifies how bridging acetates can mediate metal-metal interactions, contributing to the complex's stability and magnetic properties. Similarly, in historical pigments like , a basic copper(II) acetate such as Cu₃(CH₃COO)₄(OH)₂, the acetate ligands adopt mixed monodentate and bridging modes, providing the green coloration used in through coordination to Cu(II) sites within hydroxide-bridged chains. In catalytic applications, palladium(II) acetate, Pd(OAc)₂, features acetate ligands in a trimeric structure with bridging modes that facilitate ligand exchange and activation in the Heck reaction, where Pd coordinates to aryl halides and alkenes to form new C-C bonds under mild conditions. The acetate bridges in Pd₃(OAc)₆ provide a labile environment, enabling the precatalyst to generate active Pd(0) species. Stability constants for acetate-metal complexes vary with the metal's hardness and charge, reflecting moderate affinity typical of hard-hard interactions for metals like Cu(II) and higher values for softer transition metals like Pd(II). These constants underscore acetate's role in forming labile complexes suitable for catalysis and synthesis. Spectroscopic techniques, particularly () spectroscopy, distinguish coordination modes by analyzing the stretching vibrations. Free acetate exhibits symmetric (ν_s) and asymmetric (ν_as) O-C-O stretches at approximately 1414 cm⁻¹ and 1554 cm⁻¹, respectively, yielding Δν = ν_as - ν_s ≈ 140 cm⁻¹. In monodentate coordination, this difference increases to ~200 cm⁻¹ due to weakened symmetric bonding (e.g., ν_as ≈ 1560 cm⁻¹, ν_s ≈ 1360 cm⁻¹), while bridging bidentate modes show a smaller Δν (~80-100 cm⁻¹) from more balanced electron donation (e.g., ν_as ≈ 1570 cm⁻¹, ν_s ≈ 1430 cm⁻¹ in copper acetate). These shifts confirm the structural implications of acetate binding, aiding identification in complexes. Historically, acetate complexes were integral to alchemical practices as "salts of metals," prepared by reacting metals or oxides with vinegar (dilute acetic acid) to form compounds like lead acetate ("sugar of lead") or copper acetate (verdigris). These were subjected to dry distillation to yield "radical vinegar" (glacial acetic acid), a process documented by 16th-century alchemists such as Andreas Libavius, who used metal acetates to explore transmutation and purification. This early recognition of acetate's coordinating ability laid groundwork for later coordination chemistry.

Industrial and Other Uses

Acetic acid serves as the primary precursor for most acetate compounds and is produced industrially on a massive scale. Global production volume reached approximately 20 million metric tons in , driven largely by demand in chemicals, textiles, and solvents. The dominant manufacturing route is , exemplified by the , which reacts with under catalysis to yield acetic acid. This method accounts for the majority of worldwide capacity due to its efficiency and scalability. In the sector, acetate —particularly —are valued for their luster, breathability, and versatility in apparel and linings. Production of these fibers totaled around 0.75 million tons in 2022, representing about 13% of man-made cellulosic fiber output. This application highlights acetate's role in sustainable fabric alternatives, though it competes with synthetic fibers in . Pharmaceutical applications leverage acetate derivatives for their stability and . For instance, is widely used as an and immunosuppressive agent in ocular and systemic treatments. Additionally, acetate esters function as excipients in drug formulations, aiding in controlled release and coating processes, such as in tablet matrices. Acetate compounds contribute to environmental management through their biodegradability and utility in treatment systems. Many acetates, including short-chain varieties, degrade readily in aerobic conditions, supporting their safe disposal via processes. membranes, in particular, are applied in technologies to remove contaminants from industrial effluents, enhancing purification efficiency. Advancements in sustainable production have emerged post-2020, focusing on bio-based acetate from derived from or waste gases. Microbial using acetogenic like Moorella thermoacetica has been demonstrated at pilot scale, converting (CO, H₂, CO₂) into acetate with high selectivity and carbon-negative potential, paving the way for greener industrial alternatives.

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