Fact-checked by Grok 2 weeks ago

Bacteriophage MS2

Bacteriophage MS2 is an icosahedral, positive-sense single-stranded RNA virus belonging to the family Leviviridae that specifically infects Escherichia coli strains possessing F-pili.^[1] Its genome consists of 3,569 nucleotides encoding four proteins: a maturation protein for host attachment, a coat protein forming the capsid, an RNA-dependent RNA polymerase (replicase) for genome replication, and a lysis protein that disrupts the host cell membrane.^[1] The mature virion measures approximately 26 nm in diameter and is composed of 180 identical copies of the coat protein arranged with T=3 quasi-equivalence symmetry, encapsulating the RNA genome in a highly ordered manner.^[2] MS2 exhibits a lytic life cycle, initiating infection when the maturation protein binds to the F-pilus of the host bacterium, facilitating delivery of the genomic RNA into the cytoplasm while the empty capsid remains extracellular.^[2] Inside the cell, the positive-sense RNA serves directly as mRNA to translate the proteins, with the replicase producing both genomic and subgenomic RNAs for further protein synthesis and progeny genome amplification.^[1] Assembly occurs through specific interactions between coat proteins and stem-loop structures in the RNA genome, culminating in cell lysis to release approximately 10,000 new virions per infected cell.^[1] Discovered in 1961, MS2 was among the first RNA viruses to have its genome fully sequenced in 1976, marking a milestone in understanding genetic coding and RNA virus replication.^[3] Its simple architecture and ease of propagation have made it a foundational model in molecular biology and virology, contributing to advances in RNA packaging, translation control, and viral assembly mechanisms.^[1] Beyond basic research, MS2 and its virus-like particles (VLPs)—formed by self-assembly of coat proteins with modified RNAs—have emerged as versatile platforms for biomedical applications, including targeted delivery of antigens for vaccines against diseases like foot-and-mouth disease and human papillomavirus, as well as therapeutic RNAs for cancer and autoimmune disorders.^[1] MS2 VLPs are also employed as non-infectious surrogates in environmental virology to assess water treatment efficacy against RNA viruses and as process controls in molecular diagnostics.^[4]

Virology

Genome

Bacteriophage MS2 is a positive-sense single-stranded RNA (ssRNA+) virus belonging to the family Leviviridae, with a linear genome consisting of 3,569 nucleotides.^[5] This compact genome encodes all essential viral components in a highly efficient manner, reflecting the evolutionary pressures on small viral genomes to maximize coding capacity.^[6] The genome is organized into four major genes arranged in overlapping reading frames, which allow for the production of multiple proteins from a limited nucleotide sequence. The maturation protein A (A protein, mat gene) spans nucleotides 130 to 1,311 and initiates at AUG codon 130, terminating at UAA 1,311; it functions in virion maturation.^[5] The coat protein (cp gene) follows from nucleotides 1,335 to 1,727, starting at AUG 1,335 and ending at UAA 1,727, forming the icosahedral capsid.^[5] Overlapping with the coat gene, the lysis protein (lys gene) occupies nucleotides 1,678 to 1,905, translated in a +1 reading frame from AUG 1,678 to UAA 1,905, enabling host cell lysis.^[5] The replicase gene (rep) extends from nucleotides 1,761 to 3,398, initiating at AUG 1,761 and stopping at UGA 3,398, encoding the RNA-dependent RNA polymerase subunit critical for genome replication; its translation is translationally coupled to that of the upstream coat protein, requiring active translation of the coat gene to expose the replicase ribosome binding site.^[5]^[7] These overlaps, such as the 50-nucleotide overlap between cp and lys and the 145-nucleotide overlap between lys and rep, impose translational coupling and regulatory constraints.^[8] Non-coding regions flank the open reading frames, contributing to regulatory functions. The 5' untranslated region (UTR), spanning nucleotides 1 to 129, contains a ribosome binding site (Shine-Dalgarno sequence) upstream of the mat start codon, facilitating translation initiation of the maturation protein as the primary genomic product upon infection.^[9] The 3' UTR, from nucleotides 3,399 to 3,569, includes stem-loop structures that promote translational enhancement through ribosome recruitment and interaction with host factors, as well as serving as a binding site for the maturation protein to initiate packaging.^[9] The complete nucleotide sequence of the MS2 genome was determined in 1976 by Walter Fiers and colleagues at Ghent University, representing the first full sequencing of an RNA genome and a landmark achievement in molecular biology that enabled early insights into genetic coding and viral gene expression.^[3] This sequencing revealed the genome's secondary structure, including multiple stem-loop motifs that act as packaging signals—such as the translational operator (TR) stem-loop near the coat gene—and regulatory elements influencing replication and translation efficiency.^[3] These RNA secondary structures, confirmed through biochemical and computational analyses, are essential for selective genome encapsidation and modulation of gene expression.^[10]

Virion Structure

The virion of bacteriophage MS2 is a non-enveloped, icosahedral particle exhibiting T=3 quasisymmetry and measuring approximately 26 nm in diameter. It comprises 178 copies of the coat protein organized as 89 dimers, along with a single copy of the maturation protein, which together encapsidate the single-stranded RNA genome.^[11]^[12]^[13] The coat protein subunit consists of 129 amino acids and adopts a jelly-roll β-barrel fold, as revealed by X-ray crystallography of the virion at 2.8–3.3 Å resolution in the early 1990s. These subunits assemble into the protective capsid shell, with three quasi-equivalent conformers (A, B, and C) per icosahedral asymmetric unit to accommodate the T=3 lattice.^[14]^[15]^[16] The maturation protein (A protein), a 393-amino-acid polypeptide, occupies a unique position at one quasi-2-fold vertex, displacing a coat protein dimer from the icosahedral lattice. This protein is essential for host cell recognition, as it binds to the F-pilus on Escherichia coli during the initial stages of infection.^[17]^[13]00194-9) Encapsidation of the genomic RNA occurs via specific stem-loop structures, known as packaging signals, that bind within symmetric clefts between coat protein dimer interfaces, promoting stepwise assembly of the capsid around the RNA. The 3'-end region of the RNA facilitates recruitment of the maturation protein to complete virion formation.^[18]^[19]^[20] The mature virion demonstrates robustness, maintaining integrity at neutral pH (around 7) and temperatures up to approximately 50°C, while the intact capsid confers resistance to RNase degradation.^[21]^[22]^[23]

Replication Cycle

Bacteriophage MS2 exhibits strict host specificity, infecting only Escherichia coli strains harboring the F plasmid, which enables the expression of F-pili necessary for adsorption.^[24] The infection initiates when the maturation protein on the MS2 virion binds to the tip of the F-pilus, a filamentous structure extending from the bacterial surface.^[25] Following attachment, the virion is transported along the pilus toward the cell body, a process facilitated by pilus dynamics. Recent studies using fluorescence microscopy have revealed that uncoating occurs extracellularly, often at a distance from the cell surface along the F-pilus, where the icosahedral capsid disassembles to release the genomic RNA while the maturation protein remains associated with it; the RNA-maturity protein complex then enters the cell, likely through pilus retraction or diffusion.^[25] Upon entry, the positive-sense single-stranded RNA genome is immediately accessible to the host's translation machinery, as it functions directly as mRNA. Host ribosomes translate the genomic RNA into the viral proteins, beginning with the coat protein and replicase, whose synthesis is prioritized due to the RNA's translational control elements, such as ribosome binding sites and regulatory stem-loops.^[24] The lysis protein is also produced later in the cycle. Translation occurs in the cytoplasm, hijacking the bacterial resources without an initial eclipse period typical of some phages. Replication of the MS2 genome relies on the viral RNA-dependent RNA polymerase, known as replicase, which forms a holocomplex with host factors including elongation factors EF-Tu and EF-Ts, ribosomal protein S1, and initiation factor IF1 to enhance activity. The replicase first synthesizes a complementary negative-sense RNA strand using the positive-sense genomic RNA as a template, a process initiated at the 3' end and occurring within membrane-bound replication complexes associated with the inner bacterial membrane. These negative-sense intermediates then serve as templates for producing multiple new positive-sense progeny RNAs, amplifying the genome exponentially; host factor IF3 can inhibit this by competing for the RNA 3' ends.^[26]^[24] New virions assemble in the cytoplasm through self-assembly of the coat protein around the positive-sense RNA genomes, guided by specific packaging signals in the RNA that initiate coat protein binding and icosahedral capsid formation. The maturation protein associates with the assembled particles to complete the virion structure. Once sufficient virions accumulate, the lysis protein, a small membrane-disrupting peptide, integrates into the inner membrane, forming pores that lead to cell lysis and release of progeny phages, with no evidence of a lysogenic phase.^[24] The entire lytic cycle of MS2 typically completes in 40-60 minutes at 37°C, with a latent period of approximately 45 minutes, yielding a burst size of 5,000 to 10,000 infectious particles per infected cell under optimal conditions.^[27]^[28]

Discovery and Historical Significance

Initial Discovery

Bacteriophage MS2 was isolated in 1961 by Alvin J. Clark as a male-specific coliphage capable of infecting F+ and Hfr strains of Escherichia coli.^[29]^[30] This discovery occurred shortly after the isolation of the related RNA phage f2, highlighting a new class of small, icosahedral viruses with single-stranded RNA genomes that targeted sex-factor-mediated pili on bacterial hosts. MS2 was recognized early on for its specificity to male (F+) E. coli strains, distinguishing it from broader-host-range DNA phages like T7, and it was named MS2 to denote its male-specific infectivity.^[29] Initial characterization in the early 1960s confirmed MS2 as an RNA bacteriophage, with its genome comprising approximately 32% of the virion by weight and exhibiting sensitivity to RNase but resistance to DNase, setting it apart from DNA-based phages prevalent in prior studies such as the T-even series.^[31] Basic plaque assays were established to quantify infectivity, involving propagation on agar plates with sensitive E. coli hosts like strain Hfr 3000, allowing for routine isolation and titration of viral particles. These methods facilitated the production of high-titer lysates, essential for subsequent biochemical analyses.^[31] Research in the mid-1960s further elucidated MS2's host range and infection mechanism, revealing that adsorption required the presence of F-pili, the filamentous appendages encoded by the F plasmid that confer male-specific traits to E. coli. This pilus-dependent attachment was demonstrated through experiments showing no adsorption to F- strains and inhibition by agents disrupting pilus formation, such as sodium dodecyl sulfate. The discovery of F-pili as the receptor in 1965 provided critical insight into the ecology and specificity of RNA phages, underscoring MS2's role in probing bacterial conjugation and plasmid biology.^[32] As a founding member of the Leviviridae family (now subfamily Levivirinae), MS2 represented one of the earliest RNA bacteriophages subjected to intensive molecular scrutiny after the DNA-focused T2 era, paving the way for studies on RNA replication and translation in prokaryotes. Its small size and ease of cultivation made it an ideal model for exploring viral genetics in the post-DNA structure era, influencing virology's shift toward RNA viruses.^[29]

Key Scientific Milestones

In 1976, Walter Fiers and his team at Ghent University achieved the first complete sequencing of an RNA genome with bacteriophage MS2, determining its 3,569-nucleotide length and elucidating the primary and secondary structure of the replicase gene, which confirmed the existence of overlapping genes in RNA viruses and provided key validation for the genetic code. This breakthrough, published in Nature, marked MS2 as the first organism whose entire genome was sequenced, paving the way for understanding RNA virus genetics and inspiring subsequent genomic efforts. During the late 1980s and early 1990s, structural studies advanced significantly with X-ray crystallography revealing the icosahedral assembly of the MS2 virion and coat protein. In 1990, Lars Liljas and colleagues determined the three-dimensional structure of the MS2 capsid at 3.3 Å resolution, demonstrating its T=3 quasi-equivalence with 180 coat protein subunits forming a 270 Å diameter shell, which highlighted the conformational flexibility enabling icosahedral symmetry.^[14] Building on this, Simon E. V. Phillips and collaborators in 1995 resolved the crystal structure of the MS2 coat protein dimer at 2.0 Å, showing the β-barrel fold and RNA-binding interfaces critical for assembly, while cryo-EM complemented these findings by visualizing RNA distribution within the capsid. In the 1990s, in vitro studies of RNA packaging and self-assembly provided insights into MS2's maturation process, establishing it as a paradigm for virus-like particle formation. Researchers demonstrated that purified coat proteins could spontaneously assemble around synthetic RNAs containing specific stem-loop packaging signals, with efficiency dependent on signal location and sequence, advancing models for RNA-directed nanotechnology and biomaterial design. These experiments, often using radiolabeled RNAs, quantified packaging selectivity and revealed how electrostatic interactions drive ordered encapsidation, influencing broader applications in synthetic biology. By the 2000s, MS2 contributed to high-resolution structural biology through cryo-EM analyses of complexes involving its components and host machinery, notably aiding ribosome studies. Cryo-EM analyses in the 2000s visualized RNA packaging within the MS2 virion, providing models of RNA-protein networks that informed mRNA recognition mechanisms. This work, integrating MS2's translational elements, supported near-atomic resolutions of ribosomal states and highlighted its utility in probing RNA-protein networks within cellular contexts.^[33] MS2 has been recognized as a foundational model for positive-strand RNA viruses due to its simplicity and genetic tractability, influencing structural and functional studies of pathogens like poliovirus by providing benchmarks for genome organization and assembly pathways. Its legacy underscores advancements in virology, from genetic engineering to antiviral strategies, with seminal contributions cited in over 5,000 studies.

Applications and Modern Research

Role in Molecular Biology

Bacteriophage MS2 serves as a foundational model organism in molecular biology for investigating key processes in RNA viruses, particularly translation initiation, ribosome binding, and RNA folding dynamics. Its compact single-stranded RNA genome and well-characterized regulatory elements enable precise studies of how secondary structures in the 5' untranslated region (UTR) influence ribosomal access and initiation efficiency. For instance, the structured 5' UTR of MS2 RNA acts as a paradigm for understanding ribosome standby mechanisms and the role of upstream sequences in modulating translation yields, where inhibitory hairpins near the ribosome binding site (RBS) can be overridden by specific RNA elements to facilitate binding.^[34]^[35]^[36] The coat protein's binding to a translational operator site within the RNA further exemplifies autoregulatory feedback, repressing late-stage translation by blocking ribosome access to the coat gene RBS, a mechanism extensively dissected through in vitro binding assays.^[37] This simplicity has made MS2 instrumental in elucidating prokaryotic translation controls, contrasting with more complex eukaryotic internal ribosome entry sites while providing insights applicable to viral RNA folding pathways.^[24] In genetic studies during the 1970s and 1980s, MS2 facilitated pioneering mutagenesis and recombination experiments to map gene functions and dissect regulatory elements. Researchers employed chemical mutagenesis and in vitro recombination to generate variants, revealing the organization of its four genes (maturation, coat, lysis, and replicase) and their interdependent expression. These efforts highlighted translational coupling between the coat and lysis genes, where frameshift events ensure coordinated production, and identified cis-acting elements critical for RNA stability and replication.^[38] Such analyses established MS2 as a tractable system for probing RNA virus genetics, influencing broader understandings of polycistronic mRNA regulation without the complications of larger genomes.^[29] MS2's ease of propagation and visualization has cemented its role in virology education, serving as a standard for laboratory demonstrations of phage biology. In teaching settings, students routinely perform plaque assays on Escherichia coli lawns to quantify infectious particles, observing clear zones of lysis that illustrate host specificity via F-pilus attachment. One-step growth curves, derived from synchronized infections, further demonstrate the lytic cycle's kinetics, including eclipse and burst phases, providing hands-on insight into viral replication dynamics.^[39]^[40] These protocols, adaptable for undergraduate labs, underscore MS2's non-pathogenic nature and rapid 40-minute lifecycle, making it ideal for safe, reproducible experiments on phage-host interactions.^[41] MS2 has contributed significantly to early genomics by exemplifying overlapping genes and serving as a prototype for RNA sequencing technologies. The discovery of its lysis gene, embedded out-of-frame within the coat protein reading frame, revealed how viruses maximize coding capacity through programmed frameshifting, a finding from ribosome protection assays that detected the novel polypeptide.^[42] This 1979 breakthrough, building on MS2's complete sequencing as the first RNA genome in 1976, illuminated polycistronic strategies and inspired initial RNA-seq approaches by enabling direct enzymatic sequencing of viral transcripts.^[43] These insights into genomic compaction have informed metagenomic surveys of RNA phages, highlighting evolutionary pressures on small viral genomes.^[44] Despite its advantages, MS2's strictly lytic lifecycle imposes limitations as a molecular biology model, lacking lysogeny and thus precluding studies of prophage integration or temperate phage regulation. This obligate lysis simplifies replication analyses but restricts exploration of latency mechanisms prevalent in many bacterial viruses, potentially overlooking dormancy-related molecular processes.^[45]^[46]

Biotechnology and Biomedical Uses

Bacteriophage MS2 has emerged as a key platform in biotechnology through its virus-like particles (VLPs), formed by the self-assembly of coat proteins into non-infectious, empty icosahedral capsids approximately 27 nm in diameter. These VLPs mimic the native virion structure but lack viral genome, enabling safe encapsulation and delivery of therapeutic payloads such as drugs, peptides, and nucleic acids. In drug delivery applications, MS2 VLPs have been engineered to package chemotherapeutic agents, demonstrating targeted release in cellular environments via surface modifications like peptide ligands for specific tumor cell binding. A 2023 study optimized MS2 VLP synthesis and purification, achieving high yields suitable for scalable production in biomedical contexts.^[47]^[16]^[48] Recent advancements emphasize MS2 VLPs in vaccine development, particularly for multi-epitope platforms against infectious diseases and cancer. By genetically fusing antigenic peptides to the coat protein's AB-loop, researchers have created chimeric VLPs that elicit robust humoral and cellular immune responses without adjuvants. A 2025 review highlights their versatility in formulating vaccines against infectious diseases and tumor-associated antigens, noting superior stability and immunogenicity compared to subunit vaccines in preclinical models. These VLPs have shown promise in immunotherapy by presenting multiple epitopes to stimulate T-cell activation, with surface engineering allowing conjugation to immune checkpoint inhibitors for enhanced antitumor efficacy.^[49]^[50]^[51] In nanotechnology, MS2's RNA packaging signals—stem-loop structures that direct selective genome encapsidation—have been harnessed to create biomaterial scaffolds and gene therapy vectors. These signals enable precise loading of therapeutic RNAs into VLPs, forming nanoscale carriers for controlled release in tissue engineering applications. Surface modifications, such as chemical conjugation of targeting moieties, facilitate receptor-specific delivery, improving uptake in gene therapy for genetic disorders. For instance, MS2-based vectors have been used to deliver siRNA payloads to hepatocytes, demonstrating reduced off-target effects through packaging signal-mediated assembly. Ongoing engineering efforts integrate these VLPs with synthetic nanomaterials, yielding hybrid scaffolds for regenerative medicine.^[52]^[53]^[54] MS2 serves as a non-pathogenic surrogate in diagnostics for monitoring viral contaminants in air and water, owing to its stability and ease of quantification via plaque assays. In environmental surveillance, it models enveloped virus persistence, aiding development of detection protocols for wastewater-based epidemiology. A 2025 study evaluated ozone microdroplet inactivation of aerosolized MS2, achieving over 99% reduction at low relative humidity, which informs scalable pathogen monitoring systems for indoor air quality during outbreaks. These findings support MS2's role in validating filtration and disinfection technologies for broader viral threats.^[55]^[56] Advancements in sequencing and uncoating research leverage MS2 for engineering improved phage-based tools. Nanopore direct RNA sequencing (DRS) of MS2 in 2025 revealed dynamic transcriptional landscapes during infection, identifying epitranscriptomic modifications that enhance replication efficiency under host stress, with implications for designing synthetic RNA vectors. Concurrently, studies on extracellular uncoating—where MS2 sheds its capsid via receptor binding on Escherichia coli pili prior to entry—have elucidated pH-dependent mechanisms using fluorescence microscopy, enabling targeted modifications for stable VLP delivery systems. These insights facilitate phage engineering for precise therapeutic deployment.^[57]^[58]^[59] Therapeutically, MS2's applications are primarily through VLPs in immunotherapy rather than direct phage therapy, due to its narrow host specificity for E. coli. As adjuncts, MS2 VLPs enhance phage cocktails by delivering immunomodulators to bacterial biofilms, improving clearance in polymicrobial infections. In cancer immunotherapy, VLP-displayed antigens prime adaptive responses. Limitations in host range restrict standalone phage therapy, but VLP platforms continue to expand MS2's biomedical footprint.^[49]^[60]^[61]

References

[1]
A novel delivery platform based on Bacteriophage MS2 virus-like ...
Here we reviewed Bacteriophage MS2 virus-like particles, including introduction to their structure, their potential as a delivery platform, and their expected ...
[2]
Bacteriophage MS2 displays unreported capsid variability ... - NIH
Bacteriophage MS2 is a positive‐sense, single‐stranded RNA virus encapsulated in an asymmetric T = 3 pseudo‐icosahedral capsid. It infects Escherichia coli ...
[3]
Complete nucleotide sequence of bacteriophage MS2 RNA - Nature
Apr 8, 1976 · Bacteriophage MS2 RNA is 3,569 nucleotides long. The nucleotide sequence has been established for the third and last gene, which codes for ...
[4]
Effects of Ionic Strength on Bacteriophage MS2 Behavior and Their ...
Bacteriophage MS2 is widely used as a surrogate to estimate pathogenic virus elimination by membrane filtration processes used in water treatment.
[5]
phage MS2 genome - Nucleotide - NCBI
### Summary of Bacteriophage MS2 Genome (NC_001417)
[6]
Genomewide Patterns of Substitution in Adaptively Evolving ... - NIH
Also indicated is the gene affected and the change in amino acid sequence, if any; as MS2 has overlapping genes, some mutations affect more than one gene.
[7]
Translational interference at overlapping reading frames in ...
The two sets of gene overlap present in the RNA phage MS2 are used as a model system. We fmd that translation of an upstream cistron can fully block initiation ...
[8]
Cell-free expression of RNA encoded genes using MS2 replicase
Sep 30, 2019 · The coding part of the genome is flanked by two untranslated regions (UTRs): The 5′ UTR leader sequence (UTRL), and the 3′ UTR trailer sequence ...
[9]
Direct Evidence for Packaging Signal-Mediated Assembly of ...
Lead ion hydrolysis positions were mapped onto the MS2 genome and their nucleotide positions were compared with respect to whether cleavage occurred only in ...
[10]
Capsid protein - Escherichia phage MS2 (Bacteriophage ... - UniProt
Capsid protein self-assembles to form an icosahedral capsid with a T=3 symmetry, about 26 nm in diameter, and consisting of 89 capsid proteins dimers.
[11]
Asymmetric cryo-EM reconstruction of phage MS2 reveals genome ...
Aug 26, 2016 · The RNA forms a branched network of stem-loops that almost all allocate near the capsid inner surface, while predominantly binding to coat ...Missing: elements | Show results with:elements
[12]
Breaking the symmetry of a viral capsid - PNAS
The capsid contains 178 copies of the coat protein (CP) and a single copy of the maturation protein, which replaces one CP dimer in the icosahedral lattice (14) ...<|control11|><|separator|>
[13]
The three-dimensional structure of the bacterial virus MS2 - Nature
May 3, 1990 · The structure of the icosahedral bacteriophage MS2 has been determined to 3.3 Å resolution by X-ray crystallography.
[14]
The refined structure of bacteriophage MS2 at 2.8 A resolution
Dec 5, 1993 · Bacteriophage MS2 is an icosahedral virus with 180 copies of a coat protein forming a shell around a single-stranded RNA molecule.
[15]
Optimizing the synthesis and purification of MS2 virus like particles
Oct 6, 2021 · The MS2 VLP is a 27 nm spherical RNA bacteriophage virus-like particle, consisting of 180 identical 129-amino acid coat protein subunits of ~ 14 ...
[16]
A - Maturation protein A - Escherichia phage MS2 ... - UniProt
Sequence · Length. 393 · Mass (Da). 43,983 · Last updated. 1986-07-21 v1 · MD5 Checksum. EB31BC8DFC8A4D99265D48EF2B505684.
[17]
The RNA binding site of bacteriophage MS2 coat protein.
Feb 1, 1993 · The coat protein of the RNA bacteriophage MS2 binds a specific stem‐loop structure in viral RNA to accomplish encapsidation of the genome and translational ...
[18]
Recent Advances in Structural Studies of Single-Stranded RNA ...
Sep 23, 2023 · According to the published cryo-EM structures, MS2 efficiently assembles ~85–90% viral particles into mature virions, while Qβ only exhibited ~ ...
[19]
Bacteriophage MS2 genomic RNA encodes an assembly instruction ...
The RNA encodes a set of capsid assembly instructions, via multiple Packaging Signals (PSs). PSs facilitate formation of the protein-protein contacts in their ...
[20]
Thermal Stability of RNA Phage Virus-Like Particles Displaying ...
May 24, 2011 · These particles typically denature at temperatures around 5-10°C lower than unmodified VLPs. Even so, they are generally stable up to about 50°C ...
[21]
The presence of RNA cargo is suspected to modify the surface ...
Moreover, this effect varies differently with pH between native MS2 virions and corresponding VLPs, supporting that RNA cargo is also involved in the regulation ...
[22]
impact of chlorine and heat on the infectivity and physicochemical ...
Jun 5, 2018 · Exposure of MS2 phage to free chlorine followed by heat leads to higher inactivation, permeability to RNases and availability of hydrophobic ...
[23]
In vitro characterisation of the MS2 RNA polymerase complex ... - NIH
Mar 25, 2022 · Host proteins are identified which are required to reconstitute a functional RNA phage MS2 replication machinery, providing a promising platform for cell-free ...
[24]
https://pmc.ncbi.nlm.nih.gov/articles/PMC8956599/
[25]
Two Classes of Membrane Binding of Replicative RNA of ... - NIH
This result suggests that polymerase components or factors required for complementary-strand synthesis are bound to membrane even in the absence of divalent ...
[26]
Burst size of bacteriophage MS2 in E.coli - BioNumbers
Burst size of bacteriophage MS2 in E.coli. Range, 5,000 to 10,000 pfu/cell ... MS2 RNA the burst size in E. coli is 5000 to 10000 pfu per cell, and each ...Missing: cycle time
[27]
GROWTH OF MALE-SPECIFIC BACTERIOPHAGE IN PROTEUS ...
The burst size was 2,000 to 3,000, which is similar to that in E. coli K-12, whereas the latent period was 45 min, definitely longer than that in E. coli ...
[28]
RNA Phage Biology in a Metagenomic Era - MDPI
Jul 21, 2018 · Typically, phage infection proceeds via adsorption, penetration, replication, assembly, and release. Briefly, phages use specialized surface ...<|control11|><|separator|>
[29]
https://www.mdpi.com/1999-4915/10/7/386
[30]
RNA folding kinetics regulates translation of phage MS2 maturation ...
In vitro experiments show that ribosome binding to the maturation gene is faster than refolding of the denatured cloverleaf. This folding delay appears related ...
[31]
Secondary structure of mRNA and efficiency of translation initiation
We postulate that initiation of translation involves interaction between an activated 30S ribosomal subunit and the 5′-terminal region of a messenger RNA.
[32]
[PDF] tails act through ribosome standby to override inhibitory structure at ...
Feb 6, 2018 · Initiation is the rate-limiting step in translation. It is well-known that stable structure at a ribosome binding site (RBS) impedes initiation.<|control11|><|separator|>
[33]
In vitro characterisation of the MS2 RNA polymerase complex ...
Mar 25, 2022 · Emesvirus zinderi, commonly called bacteriophage MS2, is one of the smallest viral pathogens known with its single-stranded 3.6 kilobase (kb) ...
[34]
The Protective Effect of Virus Capsids on RNA and DNA Virus ... - NIH
Virus Stocks, Propagation, and Plaque Assays. Bacteriophages MS2, T3, and T4 were propagated and quantified with protocols that were published previously.Missing: education growth
[35]
Genetic, Structural, and Phenotypic Properties of MS2 Coliphage ...
In this study we investigated the emergence of resistance of MS2 bacteriophage to disinfection by chlorine dioxide (ClO2), and we characterized the underlying ...
[36]
MS2 Bacteriophage - A Viral Screening Tool - Microchem Laboratory
MS2 is a bacteriophage that infects “male” Eschericia coli. Male E. coli are bacterial cells capable of passing a portion of their genetic material.
[37]
Overlapping genes in rna phage: a new protein implicated in lysis
We have identified a new 75 amino acid polypeptide (L protein) following f2 phage infection of E. coli. It is encoded by an out-of-phase overlapping gene.Missing: genomics | Show results with:genomics
[38]
Genomics: From Phage to Human - Sequence - Evolution - NCBI - NIH
The first genome, that of RNA bacteriophage MS2, was sequenced in 1976, in a truly heroic feat of direct determination of an RNA sequence.
[39]
Overlapping genes in natural and engineered genomes
Oct 5, 2021 · For instance, overlapping CDSs have certain constraints on relative reading frame and sequence composition that overlaps between 5′ and 3′ UTR ...
[40]
Bacteriophage Technology and Modern Medicine - MDPI
Aug 18, 2021 · In nature, some phages have only a lytic life cycle (called virulent phages), while others can undergo both lytic and lysogenic cycles (called ...
[41]
RNA Phage Biology in a Metagenomic Era - PMC - NIH
Jul 21, 2018 · The RNA phage MS2, isolated by Alvin John Clark in 1961 and highly similar phage f2 [13], have become key models in molecular biology and ...
[42]
MS2 Virus-like Particles as a Versatile Peptide Presentation Platform
Nov 9, 2023 · Bacteriophage MS2 VLPs are nanoparticles devoid of viral genetic material and can self-assemble from the coat protein into an icosahedral capsid ...<|control11|><|separator|>
[43]
MS2 Virus-Like Particles: A Robust, Semi-Synthetic Targeted Drug ...
Aug 7, 2025 · The data suggest that the MS2 system continues to show many of the features that will be required to create an effective drug delivery system.
[44]
MS2 virus-like particles as a versatile platform for multi-disease ...
In this review, we provide a comprehensive overview of the advancements in MS2 phage coat protein VLP-based vaccine research, with a particular focus on ...
[45]
MS2 virus-like particles as a versatile platform for multi-disease ...
MS2 VLPs have gained increasing attention as a versatile and promising platform for vaccine development. This review highlights recent progress in the design ...
[46]
Full article: Virus-like particles derived from bacteriophage MS2 as ...
MS2 is a virus that targets bacteria – a bacteriophage – which is well characterized and has been developed over many years for a number of applications. It has ...<|control11|><|separator|>
[47]
[PDF] Encapsulation of Biomolecules in Bacteriophage MS2 Viral Capsids ...
In particular, the bacteriophage MS2 capsid has provided a porous scaffold for several engineered nanomaterials for drug delivery, targeted cellular imaging, ...
[48]
Recent progress in targeted delivery vectors based on biomimetic ...
Jun 7, 2021 · MS2 VNPs. MS2 capsids are another type of bacteriophage VNPs serving as the effective vehicles to package and targeted deliver chemotherapy ...<|separator|>
[49]
Phage-based delivery systems: engineering, applications, and ...
Jun 25, 2024 · Phages themselves or combined with nanocarriers can be used as carriers for drug and gene delivery. Nanomaterials and therapeutic drugs, can be ...
[50]
Aerosolized Bacteriophage MS2 Inactivation by Microdroplets of ...
This study proposes a new methodology to inactivate airborne pathogens using ozone solution microdroplets.
[51]
Aerosolized Bacteriophage MS2 Inactivation by Microdroplets of ...
Oct 17, 2025 · In this investigation, bacteriophages MS2, phi X174, phi 6, and T7 are under evaluation. The effects of ozone concentration, contact time, ...
[52]
Nanopore direct RNA sequencing (DRS) of MS2 bacteriophages in ...
Sep 22, 2025 · Here, we employed Nanopore Direct RNA sequencing (DRS) to investigate the transcriptome and epitranscriptome landscape of the MS2 in infected E.
[53]
Extracellular uncoating of bacteriophage MS2 - PMC - NIH
Jul 3, 2025 · MS2, f2, R17, and Qβ begin infection when their Mat protein binds to the outside of F-pili. Within minutes of binding, the virus uncoats, ...<|control11|><|separator|>
[54]
Extracellular uncoating of bacteriophage MS2 - PubMed - NIH
Jul 3, 2025 · In both cases, we find that uncoating can occur anywhere on the F-pili, and that MS2 usually uncoats at a distance from the cell rather than at ...Missing: mechanism | Show results with:mechanism
[55]
Bacteriophages as Targeted Therapeutic Vehicles: Challenges and ...
Phage therapy offers a promising alternative to antibiotics in addressing rising antibiotic resistance. Phages can precisely target harmful bacteria while ...
[56]
Recent Advances and Mechanisms of Phage-Based Therapies in ...
Sep 14, 2024 · The increasing interest in bacteriophage technology has prompted its novel applications to treat different medical conditions, most interestingly cancer.