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Carbonic anhydrase

Carbonic anhydrase (CA; EC 4.2.1.1) is a family of ubiquitous metalloenzymes, primarily containing zinc at their active site, that catalyze the reversible hydration of carbon dioxide (CO₂) to bicarbonate ion (HCO₃⁻) and a proton (H⁺).^[1] This reaction, which occurs at a rate up to a million times faster than the uncatalyzed process, is fundamental to carbon dioxide transport, pH regulation, and various biosynthetic pathways across all domains of life.^[2] The enzymes belong to eight distinct genetic families (α, β, γ, δ, ζ, η, θ, and ι), with structural and mechanistic differences among them, though all share the core catalytic function involving a metal-bound hydroxide nucleophile attacking CO₂.^[1] In vertebrates, the predominant α-class includes at least 16 isoforms in humans, such as cytosolic CA II and membrane-bound CA IV, each exhibiting tissue-specific expression and roles in processes like erythrocyte CO₂ transport, renal acid-base homeostasis, and aqueous humor formation in the eye.^[2] Beyond their primary activity, CAs display catalytic promiscuity, hydrolyzing esters, aldehydes, and other substrates, and have been implicated in bacterial pH regulation and virulence.^[1] Carbonic anhydrases are structurally characterized by a central β-sheet core surrounding a conical active-site cavity, approximately 15 Å deep, where the zinc ion is coordinated by three amino acid residues (histidines in α-CAs) and a water molecule that deprotonates to form the reactive hydroxide.^[1] Their physiological importance extends to respiration, where they facilitate the Bohr effect in hemoglobin, and to disease contexts, serving as therapeutic targets for glaucoma (via CA II inhibitors like acetazolamide) and cancer, where isoforms like CA IX promote tumor acidosis and metastasis.^[2] Recent discoveries, such as the ι-class in marine diatoms, highlight ongoing evolutionary diversity and biotechnological potential for CO₂ capture and industrial catalysis.^[1]

Overview

Definition and Catalyzed Reaction

Carbonic anhydrase (CA) is a family of zinc-dependent metalloenzymes that catalyze the reversible interconversion of carbon dioxide and water into bicarbonate ion and a proton.^[3]^[4] The enzyme is classified under EC 4.2.1.1 and plays a crucial role in facilitating this fundamental reaction essential for acid-base balance and respiration across diverse organisms.^[3] The catalyzed reaction is represented by the equation:

\text{CO}_2 + \text{H}_2\text{O} \rightleftharpoons \text{HCO}_3^- + \text{H}^+

This equilibrium interconversion operates in both directions: the forward reaction involves the hydration of CO₂ to form bicarbonate, while the reverse direction entails the dehydration of bicarbonate to release CO₂.^[4] The position of the equilibrium is pH-dependent, governed by the Henderson-Hasselbalch equation, where at physiological pH values above the pKₐ of carbonic acid (approximately 6.1 at 37°C), bicarbonate predominates over dissolved CO₂, with a ratio of about 20:1 at pH 7.4.^[4] Carbonic anhydrase was first identified in 1932 by Norman U. Meldrum and Francis J. W. Roughton, who isolated the enzyme from red blood cells and demonstrated its ability to accelerate CO₂ hydration. Their discovery marked the initial recognition of this enzyme as a key catalyst in blood for expediting the transport and release of CO₂.

Biological and Physiological Importance

Carbonic anhydrase (CA) is a ubiquitous metalloenzyme present in virtually all domains of life, from prokaryotes such as bacteria and archaea to eukaryotes including plants, animals, and fungi, where it exists in multiple isoforms adapted to diverse cellular environments.^[5]^[6] These isoforms, often exceeding a dozen in higher organisms like humans, enable the enzyme to perform specialized functions while sharing a core catalytic role in the reversible hydration of carbon dioxide (CO₂) to bicarbonate (HCO₃⁻) and protons.^[4] By accelerating this reaction up to 10⁶-fold over the uncatalyzed rate, CA ensures efficient CO₂ management, which is critical for maintaining metabolic homeostasis.^[7] The physiological significance of CA spans fundamental life processes, including respiration in animals, where it supports rapid CO₂ elimination to prevent acidosis, and photosynthesis in plants and algae, where it concentrates CO₂ at the site of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) to enhance carbon fixation efficiency.^[4]^[8] In metabolic pathways, CA contributes to processes like ureagenesis and gluconeogenesis by facilitating bicarbonate availability for carboxylation reactions, thereby linking carbon flux to energy production across organisms.^[9] Its broad distribution underscores an indispensable role in adapting to environmental CO₂ fluctuations, from oceanic archaea to terrestrial plants.^[10] Evolutionarily, CA represents one of the most ancient enzymes, with γ-class homologs identified in archaea and β-class variants in bacteria, suggesting its emergence in primordial ecosystems to mediate early carbon cycling and autotrophy.^[5]^[11] This conservation across billions of years highlights its foundational contribution to life's ability to harness atmospheric CO₂ for biosynthesis and energy.^[12] Mutations causing deficiency in key isoforms, particularly CA II in humans, result in autosomal recessive syndromes characterized by osteopetrosis due to impaired bone resorption, renal tubular acidosis from disrupted acid-base handling in the kidney, and cerebral calcification, illustrating the enzyme's non-redundant roles in skeletal, renal, and neurological physiology.^[13]^[14] These disorders emphasize how CA's absence disrupts CO₂-bicarbonate equilibrium, leading to systemic failures in pH regulation and ion transport essential for survival.^[15]

Molecular Structure

Overall Protein Fold

Carbonic anhydrase enzymes display a variety of three-dimensional architectures that underscore their convergent evolution, enabling the catalysis of CO₂ hydration through structurally distinct folds. In the alpha and beta classes, the core structure consists of a central β-sheet surrounded by α-helices, forming a compact globular domain that positions the active site within a deep cleft. The gamma class, by contrast, features a unique left-handed β-helix fold, which supports trimeric assembly and active site formation at subunit interfaces.^[16]^[17]^[18] These proteins are typically composed of 250–300 amino acids, yielding a molecular mass of approximately 30 kDa, and can exist as monomers in many cytosolic forms or as oligomers, such as dimers or trimers, in membrane-associated or prokaryotic variants. The overall fold contributes to structural stability, with some carbonic anhydrases from thermophilic extremophiles exhibiting enhanced thermostability due to reinforced hydrophobic cores and hydrogen bonding networks, allowing function at temperatures exceeding 80°C.33081-8/fulltext)^[19]^[20] The first crystal structure of a carbonic anhydrase was determined in the early 1970s for human carbonic anhydrase II (previously designated as CA C), at a resolution of 2 Å, unveiling a compact β-sheet-dominated fold with seven α-helices encasing the central β-strands. Subsequent structures across classes have confirmed the evolutionary divergence in folds while highlighting conserved active site geometries that facilitate zinc coordination. Post-translational modifications, such as N-linked glycosylation in certain membrane-bound isoforms like CA IX and CA XII, influence folding efficiency, stability, and cellular localization without altering the core architecture.^[21]

Active Site Configuration and Metal Ion Role

The active site of carbonic anhydrase is a conical cavity approximately 15 Å deep, featuring a central zinc ion (Zn²⁺) that coordinates the catalytic chemistry. In the alpha class, exemplified by human carbonic anhydrase II (CA II), the Zn²⁺ ion adopts a tetrahedral geometry, bound to the imidazole nitrogen atoms of three conserved histidine residues—His94, His96, and His119—and a fourth ligand consisting of a water molecule or hydroxide ion depending on pH.^[22]^[23]^[24] This coordination environment positions the zinc-bound water/hydroxide for nucleophilic attack on the substrate. Surrounding the zinc center, key residues such as Thr199 and Glu106 form a hydrogen-bonding network with the bound water/hydroxide, stabilizing the nucleophile and orienting it for catalysis while facilitating substrate binding.^[25]^[26] The active site also includes a hydrophobic pocket lined by residues like Val121, Val143, and Leu198, which accommodates the nonpolar CO₂ substrate and promotes its diffusion into the reactive region.^[27]^[28] The zinc ion plays a pivotal role in modulating the pKa of the bound water from approximately 15.7 (free water) to around 7, generating a zinc-bound hydroxide nucleophile at physiological pH that is essential for efficient CO₂ hydration.^[29]^[30] This pKa shift arises from the electrostatic stabilization provided by the positively charged Zn²⁺ and the protein environment, enabling the enzyme's high turnover rates. Structural details of the active site have been elucidated through X-ray crystallography and extended X-ray absorption fine structure (EXAFS) spectroscopy, revealing average Zn-N (histidine) bond lengths of 1.98–2.02 Å and Zn-O (water/hydroxide) distances of 2.10–2.15 Å in CA II.^[22]^[23] These measurements confirm the tetrahedral coordination and provide insights into the electronic environment influencing reactivity. While zinc is the predominant metal cofactor across carbonic anhydrase classes, rare alternatives exist in certain bacterial and archaeal forms; for instance, cadmium substitutes for zinc in delta-class enzymes from marine diatoms like Thalassiosira weissflogii, and iron serves as the native cofactor in gamma-class enzymes from Methanosarcina thermophila.^[31]^[32]^[33] These substitutions maintain catalytic function but are adapted to specific environmental metal availabilities.

Catalytic Mechanism

Reaction Overview and Kinetics

Carbonic anhydrase catalyzes the reversible hydration of CO₂ to bicarbonate and a proton, following Michaelis-Menten kinetics with high efficiency, particularly in the α-class isoform CA II, which serves as a benchmark for enzymatic performance. For CA II, the turnover number (k_cat) reaches approximately 1.3 × 10⁶ s⁻¹ at pH 7.5 and 37°C, making it one of the fastest known enzymes and approaching the diffusion limit for substrate binding.^[34] The Michaelis constant (K_m) for CO₂ is low, around 10 mM, indicating high affinity and specificity for the substrate under physiological conditions where CO₂ concentrations are typically much lower.^[34] This combination of parameters enables CA II to process substrates at near-maximal rates even at subsaturating concentrations, underscoring its role in rapid metabolic flux. The enzyme achieves a rate enhancement of about 10⁷-fold compared to the uncatalyzed reaction, transforming a kinetically sluggish process into one suitable for biological timescales. The uncatalyzed hydration of CO₂ at pH 7 and 25°C proceeds with a pseudo-first-order rate constant of approximately 0.037 s⁻¹, limited by the formation of the transition state involving water attack on CO₂.^[35] In contrast, CA II accelerates this to 10⁶ s⁻¹, effectively overcoming the activation barrier through metal ion coordination and residue stabilization, without altering the equilibrium constant of the reaction. The catalytic activity of carbonic anhydrase exhibits pH dependence, with optimal performance in the physiological range of pH 7-8, displaying a bell-shaped profile that reflects the ionization states of key active site residues. At lower pH, protonation of the zinc-bound hydroxide reduces nucleophilicity, while at higher pH, deprotonation of proton shuttle residues like His64 impairs product release; the curve peaks where both species are optimally balanced for catalysis.^[34] Isotope exchange studies using ¹³C and ¹⁸O have elucidated rate-limiting steps, confirming that proton transfer from the active site often governs overall turnover in efficient isoforms like CA II. For instance, ¹⁸O exchange kinetics reveal multiple proton-transfer events contributing to the observed k_cat, with the enzyme's efficiency deriving from a network of shuttles that minimize this limitation, while ¹³C effects highlight CO₂ binding as diffusion-controlled.^[36]

Detailed Proton Transfer and Hydration Steps

The catalytic mechanism of carbonic anhydrase proceeds via a ping-pong bi-bi pathway, where the enzyme alternates between two states to facilitate the reversible hydration of CO₂ to HCO₃⁻ and H⁺. In the first phase, a zinc-bound hydroxide ion (Zn-OH⁻) acts as a nucleophile, attacking the carbon atom of CO₂ to form a bicarbonate adduct (Zn-HCO₃⁻) with a rate constant approaching 10⁶ s⁻¹. This step occurs within the hydrophobic pocket near the active site zinc, where CO₂ binds loosely without direct coordination to the metal.^[37]^[38] Following the nucleophilic addition, the bicarbonate ion is released from the zinc coordination sphere, displacing it into solution and leaving a zinc-bound water molecule (Zn-H₂O). This deprotonated water then requires regeneration of the active Zn-OH⁻ nucleophile for the next cycle, which involves a critical intramolecular proton transfer. The proton from Zn-H₂O is shuttled to the bulk solvent via His64, a conserved histidine residue positioned at the active site entrance. His64 rotates between inward and outward conformations, facilitating proton acceptance from the active site water via a hydrogen-bonded network of solvent molecules and subsequent release to the exterior.^[37]^[39]^[40] Key intermediates in this process include the Zn-OH⁻ nucleophile, which is stabilized by the Lewis acidity of the Zn²⁺ ion, the transient bicarbonate adduct bound bidentately to zinc, and the protonated form of His64 (His64H⁺). The tautomerism of His64, with equal populations of its Nδ1-H and Nε2-H forms, enables efficient proton relay without requiring large-scale conformational shifts, occurring through a concerted mechanism involving water bridges at neutral pH.^[39]^[38] The rate-limiting step varies depending on conditions but often involves the proton transfer mediated by His64, particularly in the regeneration of Zn-OH⁻, with free energy barriers around 10.4 kcal/mol as determined by density functional theory (DFT) calculations. These quantum mechanical studies, using hybrid functionals like B3LYP, reveal transition states for proton shuttling via Grotthuss-like water chain mechanisms and the subsequent deprotonation, highlighting low barriers (∼4.4 kcal/mol) for the CO₂ attack itself compared to proton handling. In some cases, product release or substrate diffusion can become limiting, underscoring the enzyme's optimization for rapid turnover.^[38]^[40]

Classification into Families

Alpha-Carbonic Anhydrases

Alpha-carbonic anhydrases (α-CAs) represent the predominant class of carbonic anhydrases in vertebrates, characterized by a distinctive protein fold consisting of a central twisted β-sheet typically comprising 8 to 10 strands, surrounded by α-helices that form a conical cavity housing the active site.^[17] This architecture facilitates the coordination of a zinc ion at the active site, essential for catalysis, and distinguishes α-CAs from other classes through their right-handed β-α-β motif arrangement within the core structure.^[41] In humans, 15 α-CA isoforms have been identified (CA I–XV), of which 12 are catalytically active, varying in subcellular localization, tissue distribution, and kinetic properties.^[42] Among the key isoforms, CA II is a highly efficient cytosolic enzyme abundant in red blood cells (RBCs), where it accelerates CO₂ hydration to support rapid gas exchange during respiration.^[43] CA IX, a transmembrane isoform, is uniquely induced under hypoxic conditions via hypoxia-inducible factor-1 (HIF-1) and is overexpressed in various tumors, contributing to pH regulation in the tumor microenvironment.^[44] Another notable isoform, CA IV, is GPI-anchored to the extracellular surface and prominently expressed in the lungs and kidneys, aiding in bicarbonate transport across endothelial barriers.^[45] Expression of α-CAs is ubiquitous across human tissues but exhibits isoform-specific patterns, such as CA IV's enrichment in pulmonary endothelium to facilitate alveolar gas exchange.^[46] Mutations in the CA II gene lead to carbonic anhydrase II deficiency syndrome, an autosomal recessive disorder also known as marble brain disease, characterized by osteopetrosis, renal tubular acidosis, and intracranial calcifications due to impaired acid-base homeostasis.^[47] Recent advances include the computational design of selective CA IX inhibitors, such as pharmacophore-based screening of small-molecule libraries yielding compounds with high affinity for the tumor-associated isoform while sparing off-target CAs, offering promise for targeted cancer therapies.^[48]

Beta- and Gamma-Carbonic Anhydrases

Beta-carbonic anhydrases (β-CAs) represent a distinct class of zinc metalloenzymes characterized by a left-handed β-helix fold, consisting of twisted parallel β-sheets that form the core structure of each monomer.^[8] These enzymes are prevalent in prokaryotes, plants, algae, and some archaea, where they catalyze the reversible hydration of CO₂ to bicarbonate and protons. In plants, β-CAs are commonly localized in chloroplasts, such as the Cab-type β-CA in Pisum sativum, which plays a crucial role in photosynthesis by facilitating CO₂ concentration near Rubisco, thereby enhancing carbon fixation efficiency in CO₂-limited environments.^[49] The functional unit of many β-CAs is a dimer, with the active site formed at the interface between subunits; the catalytic zinc ion is coordinated by two cysteines, one aspartate, and one histidine, enabling a pH-dependent conformational switch that activates the enzyme at higher pH values.^[50] Catalytic turnover rates (k_cat) for β-CAs can reach up to 4 × 10⁵ s⁻¹, approaching the efficiency of some α-class enzymes.^[51] Evolutionarily, β-CAs trace their origins to ancient prokaryotic lineages, where they were co-opted into CO₂-concentrating mechanisms, such as carboxysomes in bacteria and cyanobacteria, to optimize Rubisco activity by maintaining elevated bicarbonate levels.^[12] This adaptation likely preceded the endosymbiotic event that integrated prokaryotic ancestors into plant chloroplasts, allowing β-CAs to support photosynthetic carbon assimilation in higher plants.^[8] Unlike α-CAs, β-CAs uniquely exhibit allosteric regulation in some variants, where substrate binding modulates activity through proton loss and conformational changes.^[51] Gamma-carbonic anhydrases (γ-CAs) form another evolutionarily unrelated class, featuring a compact left-handed parallel β-helix fold with three strands per turn, distinct from the more extended helix in β-CAs.^[32] The prototype γ-CA, Cam from the archaeon Methanosarcina thermophila, functions as a homotrimer with active sites at subunit interfaces, where a zinc (or sometimes iron) ion is coordinated by three histidine residues.^[52] These enzymes are primarily found in archaea and some bacteria, contributing to methanogenesis by aiding acetate metabolism through CO₂ hydration, which helps maintain intracellular pH and provides bicarbonate for biosynthetic pathways.^[53] Catalytic activity is notably slower than in β-CAs, with k_cat values exceeding 10⁴ s⁻¹ but limited by inefficient proton transfer.^[32] A key mechanistic difference in γ-CAs is the absence of a histidine-based proton shuttle, as seen in α-CAs; instead, proton transfer relies on a network of glutamate residues, such as Glu84 in Cam, which facilitates deprotonation but results in lower overall efficiency compared to the histidine-mediated shuttling in other classes.^[54] This structural variation underscores the convergent evolution of carbonic anhydrases across domains of life, where γ-CAs represent an ancient adaptation suited to extremophilic environments like those of methanogenic archaea.^[32]

Other Classes (Delta, Zeta, Eta, Iota)

The delta class of carbonic anhydrases (δ-CAs) is predominantly found in marine diatoms, serving as key extracellular enzymes in carbon acquisition under low-CO₂ conditions. These enzymes are cambialistic, capable of incorporating either zinc (Zn^II) or iron (Fe^II) as the catalytic metal ion, allowing adaptation to varying metal availabilities in oceanic environments. The δ-CA from the diatom Thalassiosira weissflogii, designated TweCA, is a monomeric protein comprising 281 amino acids with a molecular mass of approximately 32 kDa, featuring a Zn^II ion coordinated in a manner akin to α-CAs but with distinct phylogenetic positioning. TweCA exhibits efficient CO₂ hydration activity, achieving a turnover number (k_cat) of 1.3 × 10⁵ s^-1 and a second-order rate constant (k_cat/K_M) of 3.3 × 10⁷ M^-1 s^-1, while lacking esterase activity toward 4-nitrophenyl acetate. Notably, TweCA displays high thermostability, retaining about 40% activity after incubation at 80°C for 30 minutes, which supports its role in the dynamic marine habitat.^[55]^[56] The zeta class (ζ-CAs) occurs in marine algae, such as species of Porphyra and diatoms like Phaeodactylum tricornutum, as well as in certain bacteria, where they contribute to intracellular CO₂ concentration mechanisms. Unlike most CA classes, ζ-CAs lack the canonical histidine ligands for metal coordination, instead employing two cysteine residues and one histidine residue to bind the Zn^II ion within a left-handed β-helix fold, which facilitates a unique catalytic pocket. This atypical coordination enables efficient interconversion of CO₂ and HCO₃^-, with k_cat values reaching up to 10⁶ s^-1 in algal isoforms. Beyond CO₂ hydration, ζ-CAs demonstrate CS₂ hydrolase activity, hydrolyzing carbon disulfide to COS and H₂S, suggesting broader roles in sulfur metabolism for microbial sulfur-oxidizing pathways. Their presence in extremophilic bacteria further underscores adaptability to low-pH or high-sulfide environments.^[57]^[58] The eta class (η-CAs), first characterized in the mid-2010s, has been identified in bacteria and protozoan pathogens, featuring a protein fold that closely resembles α-CAs but with a distinctive active site architecture. The Zn^II ion in η-CAs is coordinated by two histidine residues and one glutamine residue, along with a water/hydroxide nucleophile, deviating from the three-histidine motif of canonical classes and imparting specificity to anion inhibition profiles. This unique geometry supports robust CO₂ hydration without accompanying esterase function, with sulfonamide inhibitors showing moderate potency compared to α-CAs. η-CAs' bacterial representatives, such as those in soil and pathogenic species, highlight their potential in microbial pH homeostasis and as targets for antibacterial therapies. Phylogenetic analyses of signal peptides indicate predominant periplasmic localization in Gram-negative bacteria, aiding extracellular CO₂ capture.^[59]^[60] The theta class (θ-CAs), discovered in 2016, is found in marine diatoms such as Phaeodactylum tricornutum, where it localizes to the thylakoid lumen and plays a critical role in photosynthesis. θ-CAs belong to the Cys-Gly-His-rich family and feature a zinc-binding site coordinated by two cysteine residues (Cys307, Cys387), one aspartate (Asp309), and two histidine residues (His349, His363). The enzyme exhibits CO₂ hydration activity of approximately 30.9 Wilson-Anderson units (WAU) per mg protein and bicarbonate dehydration activity of 42.2 WAU per mg protein, with moderate esterase activity. θ-CAs are essential for growth and photosynthetic efficiency, utilizing the thylakoid pH gradient to supply CO₂ to Rubisco and regulate dissolved inorganic carbon/proton equilibria in the lumen.^[61] The iota class (ι-CAs), emerging as a distinct group in the early 2020s, was initially discovered through genomic analysis of the marine diatom Thalassiosira pseudonana, where the enzyme LCIP63 functions in CO₂-concentrating mechanisms essential for photosynthesis in low-CO₂ seawater. ι-CAs exhibit a novel fold unrelated to established classes, with the catalytic metal—preferentially Mn²⁺ but also active with Zn^II or Cd²⁺—coordinated in a geometry that enables high-efficiency hydration, though specific kinetic parameters vary by isoform (e.g., k_cat ~10⁵–10⁶ s^-1). Subsequent identification in marine and soil bacteria, including Burkholderia territorii (BteCAι), reveals widespread distribution among prokaryotes, suggesting evolutionary convergence for metal flexibility in nutrient-limited niches. These enzymes' potential for alternative mechanisms, such as enhanced anion binding or cofactor promiscuity, positions them for biotechnological applications in carbon capture.^[62]^[63] Genomic and metagenomic surveys conducted between 2023 and 2025 have expanded understanding of δ-, ζ-, η-, and ι-CA diversity, particularly in extremophiles inhabiting marine hydrothermal vents and other high-temperature, low-pH environments. These studies identified novel clades and biome-specific variants, revealing higher prevalence of ι-CAs in coastal microbial mats and ζ-CAs in sulfur-rich sediments, with adaptations like altered metal-binding motifs enhancing stability under extreme conditions. For instance, analyses of thermophilic microbiomes uncovered ι-like sequences in archaea-bacteria consortia, emphasizing their role in global carbon cycling and potential for engineering heat-resistant variants. Such findings underscore the untapped microbial reservoir of these classes for addressing climate-related challenges.^[64]^[65]

Physiological Roles

pH Regulation and Acid-Base Balance

Carbonic anhydrase (CA) plays a pivotal role in the bicarbonate buffer system, which is essential for maintaining pH homeostasis in various tissues by facilitating the interconversion of carbon dioxide and bicarbonate to buffer protons. In the kidneys and stomach, CA generates bicarbonate ions (HCO₃⁻) that neutralize excess H⁺, preventing acidification of cellular and extracellular fluids. This enzymatic activity supports the rapid production and reabsorption of bicarbonate, ensuring acid-base balance during physiological processes that produce metabolic acids.^[66] In the renal proximal tubule, cytosolic CA II and membrane-bound CA IV are critical for bicarbonate reabsorption, accounting for approximately 80-90% of filtered HCO₃⁻ recovery. CA II, comprising about 95% of renal CA activity, catalyzes the intracellular formation of H⁺ and HCO₃⁻ from CO₂ and H₂O, enabling H⁺ secretion via the apical Na⁺/H⁺ exchanger (NHE3) and HCO₃⁻ efflux through the basolateral Na⁺/HCO₃⁻ cotransporter (kNBC1). CA IV, anchored on both apical and basolateral membranes via a glycosylphosphatidylinositol (GPI) linkage, accelerates the dehydration of filtered H₂CO₃ to CO₂ on the luminal side and enhances basolateral HCO₃⁻ export, forming functional metabolons with transporters. Defects in CA II, such as in carbonic anhydrase II deficiency syndrome, impair H⁺ secretion and bicarbonate regeneration, leading to proximal renal tubular acidosis and metabolic acidosis. Similarly, inhibition or absence of CA IV reduces bicarbonate reabsorption efficiency, exacerbating acid-base imbalances.^[66]^[67] Cytosolic CA isoforms, particularly CA II, are essential for intracellular pH regulation by mitigating acidification from metabolic CO₂ production during cellular respiration and other processes. These enzymes rapidly convert metabolically generated CO₂ to HCO₃⁻ and H⁺, allowing H⁺ extrusion via membrane transporters and maintaining cytosolic pH near neutrality to support enzymatic functions and prevent metabolic stress. In various cell types, including neurons and hepatocytes, cytosolic CA activity buffers pH fluctuations, with inhibition leading to slowed pH recovery and altered cellular responses to acid loads. Membrane-associated CA IV can also exhibit intracellular activity, contributing to proton buffering in the cytoplasm of specialized cells like neurons.^[68]^[69] In gastric parietal cells, CA IV supports hydrochloric acid (HCl) secretion by facilitating proton production and bicarbonate handling on the cell membranes. Expressed on the basolateral and potentially apical surfaces, CA IV catalyzes CO₂ hydration to supply H⁺ for the H⁺/K⁺-ATPase pump and enables HCO₃⁻ extrusion to the bloodstream, preventing intracellular alkalization. This membrane-bound activity, which retains function in acidic environments (pH < 4), contributes significantly to stimulated acid secretion, with inhibition reducing H⁺ output by up to 55% in experimental models.^[70] Recent studies have explored carbonic anhydrase inhibitors (CAIs) in regulating vascular tone, highlighting their potential beyond traditional uses. Sulfonamide-based CAIs induce vasodilation in cerebral, ocular, and renal vasculatures by modulating local pH and ion transport, increasing blood flow without affecting abdominal vessels; this effect depends on inhibitor lipophilicity and membrane permeability. Coumarin-type CAIs exhibit similar vasodilatory actions on retinal arterioles, suggesting therapeutic applications in vascular pH-related disorders.^[71]

Carbon Dioxide Transport and Respiration

Carbonic anhydrase isoforms I and II, predominantly expressed in erythrocytes, catalyze the rapid conversion of carbon dioxide (CO₂) produced by tissues into bicarbonate (HCO₃⁻) and protons (H⁺), enabling efficient CO₂ transport in plasma.^[72] This hydration reaction, accelerated by up to 13,000–25,000-fold by these cytosolic enzymes, allows 95% completion within milliseconds, preventing diffusion limitations during gas exchange.^[72] The resulting HCO₃⁻ is then exchanged for chloride (Cl⁻) across the erythrocyte membrane via the band 3 anion exchanger (also known as AE1), which operates at a turnover rate of 40,000–50,000 ions per second, facilitating HCO₃⁻ efflux into plasma for systemic transport while maintaining electroneutrality.^[72] Approximately 70–80% of total CO₂ is carried as HCO₃⁻ in this form, underscoring the enzyme's critical role in venous return.^[73] This process is further enhanced by the Haldane effect, where deoxygenation of hemoglobin in peripheral tissues increases its affinity for H⁺ and CO₂, promoting greater HCO₃⁻ formation; carbonic anhydrase facilitates this linkage by accelerating the interconversion kinetics between CO₂, HCO₃⁻, and H⁺, thereby amplifying overall CO₂ loading capacity by up to 20–30% without the enzyme's slowdown.^[72] In the lungs, the reverse occurs: membrane-bound carbonic anhydrase IV (CA IV) on pulmonary endothelial and alveolar epithelial cells dehydrates incoming HCO₃⁻ to regenerate CO₂, which diffuses into alveoli for exhalation, completing the respiratory cycle with near-complete efficiency.^[74] This extracellular isoform ensures rapid reversal of the peripheral hydration, with activity localized to facilitate HCO₃⁻ influx from plasma and H⁺ buffering by oxygenated hemoglobin via the Haldane effect.^[73] During exercise, when CO₂ production surges, blood carbonic anhydrase activity upregulates, with studies showing up to a 50% increase in erythrocyte CA levels after two weeks of high-intensity training, supporting faster CO₂ clearance and preventing hypercapnia.^[75] This adaptive response enhances isoform expression or activation to match elevated metabolic demands, maintaining ventilatory efficiency.^[76] More recently, in obstructive sleep apnea, elevated CA activity correlates with increased arterial HCO₃⁻ levels and hypoxemic burden; pharmacological reduction of CA activity has been linked to amelioration of apnea severity, potentially by lowering HCO₃⁻ accumulation and improving respiratory control.^[77]^[78]

Inhibitors and Therapeutics

Types of Inhibitors and Their Mechanisms

Carbonic anhydrase (CA) inhibitors are classified based on their chemical nature and binding modes, primarily targeting the enzyme's active site zinc ion or occluding the substrate access channel. The main types include sulfonamides, anions, coumarins, and emerging polypharmacological hybrids, each exhibiting distinct inhibition mechanisms such as competitive, uncompetitive, or non-competitive binding. These inhibitors generally achieve nanomolar to micromolar potencies against human CA isoforms, with selectivity influenced by structural variations.^[79] Sulfonamides represent the classical class of CA inhibitors, characterized by a sulfonamide group that binds directly to the zinc ion in the active site. The deprotonated nitrogen of the sulfonamide coordinates to Zn(II), displacing the catalytically essential hydroxide ion (OH⁻) and forming a stable tetrahedral complex that prevents substrate binding. This binding mode results in uncompetitive inhibition with respect to CO₂ hydration, as the inhibitor preferentially associates with the enzyme-substrate complex. Acetazolamide, a prototypical heterocyclic sulfonamide, exemplifies this class with an inhibition constant (Kᵢ) of approximately 12 nM against human CA II (hCA II), highlighting its high affinity.^[80]^[79] Anions constitute another early-discovered class, acting as inorganic or simple organic inhibitors that directly coordinate to the Zn(II) ion. Monovalent anions such as cyanide (CN⁻) and iodide (I⁻) bind to the zinc in a tetrahedral geometry, replacing the OH⁻ ligand and thereby blocking the nucleophilic attack on CO₂. This coordination leads to uncompetitive inhibition at physiological pH, with nonlinear kinetics observed in Dixon plots for most anions except cyanate. Cyanide, often studied in complexes like Au(CN)₂⁻, exhibits particularly strong binding due to its nucleophilic properties, while iodide shows weaker but pH-dependent inhibition.^[81]^[79] Coumarins form a newer class of non-zinc-binding inhibitors that operate via a unique prodrug mechanism. These compounds initially bind near the active site entrance, where the enzyme's intrinsic esterase activity hydrolyzes the lactone ring to generate a 2-hydroxycinnamic acid derivative. This activated form then reorients to occlude the active site channel, preventing substrate entry in a non-competitive manner. Coumarins demonstrate remarkable isoform selectivity, potently inhibiting tumor-associated isoforms like CA IX and XII (Kᵢ values in the nanomolar range) while sparing cytosolic CA I and II, making them valuable for targeted applications.^[82]^[79] Recent advances in polypharmacology have yielded dual CA inhibitors that simultaneously target CA and other enzymes, enhancing therapeutic potential through multifaceted binding. For instance, hybrids designed to inhibit CA alongside monoamine oxidases (MAO) or cholinesterases (ChE) exhibit competitive zinc-binding at CA while modulating secondary targets via distinct pharmacophores, with 2024-2025 developments focusing on brain-penetrant compounds for neurodegenerative disorders (IC₅₀ < 100 nM for hCA II). In 2024, novel hydantoin-phthalimide hybrids were synthesized via condensation of activated phthalimides with 1-aminohydantoin, acting as potent, selective inhibitors of isoforms CA VI, VII, and IX (Kᵢ values 10-50 nM) through zinc coordination similar to sulfonamides. These innovations underscore ongoing efforts to refine inhibition mechanisms for isoform-specificity.^[83]^[84]

Clinical Applications and Emerging Therapies

Carbonic anhydrase inhibitors (CAIs) have established clinical applications in ophthalmology, particularly for glaucoma management, where topical formulations such as dorzolamide and brinzolamide reduce intraocular pressure by decreasing aqueous humor production.^[85]^[86] These agents are administered as eye drops, typically two to three times daily, and are often used in combination with other antiglaucoma therapies to enhance efficacy in patients with open-angle glaucoma or ocular hypertension.^[85] Systemic CAIs like acetazolamide may also be employed in refractory cases or congenital glaucoma to lower intraocular pressure prior to surgery.^[87] In neurology, acetazolamide serves as an adjunctive therapy for certain epilepsy syndromes, including absence seizures and familial periodic paralysis with epilepsy, by modulating neuronal pH and excitability to prevent and control seizures.^[88]^[89] It is also utilized for migraine prophylaxis, particularly in conditions like CADASIL-associated migraine with aura, where it reduces attack frequency and severity, though the exact mechanism remains under investigation.^[90]^[91] In oncology, selective inhibitors targeting carbonic anhydrase IX (CAIX), such as SLC-0111, are being explored for hypoxic tumors, where CAIX overexpression supports tumor survival and resistance to therapy. SLC-0111, currently in Phase Ib/II clinical trials as of 2025, sensitizes cancer cells to chemotherapeutics like temozolomide and dacarbazine in glioblastoma and melanoma models, respectively, by disrupting pH regulation in the tumor microenvironment.^[92]^[93]^[94]^[95] This approach aims to curtail tumor growth and metastasis in cancers like gastric and renal cell carcinoma.^[96] Emerging therapies highlight CA modulation's potential beyond traditional indications. A 2024 study demonstrated that CA inhibition ameliorates tau toxicity in Alzheimer's disease models by enhancing tau secretion and reducing intracellular aggregation, with no observed toxicity in treated mice, suggesting repurposing of existing CAIs like acetazolamide.^[97] In sleep apnea, 2025 clinical data link reduced CA activity to amelioration of obstructive sleep apnea (OSA), with the CAI sultiame showing dose-dependent improvements in apnea-hypopnea index and ventilatory stability in Phase II trials, positioning it as a novel oral therapy.^[77]^[98] A 2024 review underscores CA's role in vascular tone regulation, where CAIs influence endothelial and smooth muscle function to modulate blood flow, offering therapeutic promise for cardiovascular disorders.^[99] Dual-target CAIs represent an advancing strategy for multifactorial diseases, combining CA inhibition with other pathways to enhance outcomes in cancer and inflammation; a 2025 analysis highlights their potential to overcome resistance and reduce side effects by addressing tumor acidosis and inflammatory cascades simultaneously.^[100] Systemic CAI use is limited by side effects, including transient diuresis from renal bicarbonate loss and paresthesia manifesting as tingling in extremities or perioral regions, which occur due to metabolic acidosis and electrolyte shifts.^[101]^[102]^[103] These effects often necessitate dose adjustments or topical alternatives to improve tolerability.^[104]

Biotechnological Applications

Carbon Capture and Sequestration

Carbonic anhydrase (CA) enzymes are employed in bioreactors to accelerate the hydration of CO₂ to bicarbonate (HCO₃⁻), facilitating its mineralization into stable carbonates such as calcium carbonate (CaCO₃) in the presence of divalent cations like Ca²⁺. This process mimics natural biomineralization, where CA catalyzes the rate-limiting step of CO₂ hydration, enabling rapid precipitation of CaCO₃ from CO₂-laden solutions containing CaCl₂. In experimental setups, immobilized CA on gas-permeable membranes within bubble-column reactors has demonstrated enhanced CO₂ sequestration, with precipitation rates increasing significantly compared to uncatalyzed systems, producing up to 920 mg of CaCO₃ per mg of enzyme over multiple cycles.^[105] For post-combustion capture, CA is immobilized on membranes or hollow fibers to enhance flue gas scrubbing, particularly in alkaline absorbents like monoethanolamine (MEA) or potassium carbonate (K₂CO₃). These configurations promote CO₂ absorption by accelerating bicarbonate formation, with evolved CA variants achieving up to 25-fold increases in mass transfer coefficients (from 1.6 to 39 kmol·m⁻³·atm⁻¹·h⁻¹) in 2.1 M methyldiethanolamine (MDEA) solutions. Immobilization methods, such as covalent attachment to magnetic nanoparticles or encapsulation in chitosan-alginate beads, retain 50–60% activity after 10–22 cycles, reducing absorber column sizes by over 90% relative to conventional amine systems.^[106]^[105] Key challenges in deploying CA for carbon capture and sequestration (CCS) include enzyme stability under high temperatures (up to 107 °C), alkaline pH (>10), and chemical inhibitors in flue gases, as well as high production costs limiting scalability. Directed evolution has addressed these by introducing mutations (e.g., 36 sites altered in variants like DvCA8.0, including A56S and A84Q) that extend half-life from 15 minutes to 14 weeks at 50 °C, a 4,000,000-fold stability gain over wild-type enzymes. Despite these advances, enzyme leaching and matrix destabilization by bicarbonates persist, necessitating further engineering for cost-effective, recyclable systems.^[106]^[105] Field trials have validated CA's efficacy, including a 2014 pilot at the National Carbon Capture Center using evolved DvCA, which sustained 60–70% CO₂ capture over 60 hours across absorption-desorption cycles at 25–87 °C. More recently, Veolia's 2024 world-first trial in the UK applies CA enzymes combined with silicate rocks for enhanced weathering on agricultural land, accelerating natural CO₂ mineralization to bicarbonate and silicates for permanent storage. In 2023, Saipem launched Bluezyme, a biomimetic nanomaterial-based CA solution for industrial CO₂ capture, demonstrating thermo- and alkali-stability in harsh conditions to support scalable CCS deployment.^[106]^[107]^[108] CA-assisted CCS holds potential to contribute to IPCC targets by enabling gigaton-scale annual CO₂ capture, aligning with the median 196 Gt of fossil CO₂ cumulatively captured and stored across low-overshoot 1.5 °C pathways (AR6 WG3), through energy-efficient processes that cut desorption energy by up to fourfold and environmental impacts by 50–54% compared to traditional methods.^[109]^[105]

Biomimetic and Industrial Uses

Biomimetic approaches to carbonic anhydrase (CA) have focused on synthetic catalysts that replicate the enzyme's zinc-based active site to accelerate CO₂ hydration, facilitating downstream conversion to value-added products such as fuels. Zinc-cyclen complexes, for instance, serve as effective mimics by coordinating Zn(II) ions within a macrocyclic ligand to catalyze CO₂ hydrolysis into bicarbonate at rates approaching natural CA efficiency under mild conditions.^[110] These systems have been integrated into ionic liquids to enhance solubility and stability, enabling continuous CO₂ processing for potential fuel synthesis pathways.^[111] A bifunctional Zn(cyclen)²⁺ variant, for example, boosts CO₂ capture rates by 84% while suppressing competing hydrogen evolution reactions during electrochemical reduction, supporting integrated conversion to formate or other C1 fuels.^[112] In industrial settings, CA enzymes aid pH control in desalination processes by rapidly converting CO₂ to bicarbonate, mitigating scaling and corrosion in membrane systems.^[113] For food processing, CA accelerates CO₂ carbonation in beverages, enabling efficient dissolution without excessive pressure or energy, as demonstrated in membrane-based reactors that produce sparkling water and soft drinks.^[114] In biofuel production, CA enhances CO₂ fixation in photosynthetic organisms like cyanobacteria and algae, increasing biomass yields by up to 30-50% through improved carbon flux; for example, expression of human CA II in algal chloroplasts has boosted growth rates for biodiesel precursors.^[115] Enzyme engineering via directed evolution has produced thermostable CA variants suitable for harsh industrial environments. A β-class CA from a thermophilic source was iteratively mutated to achieve over 4,000,000-fold greater stability at temperatures exceeding 80°C and alkaline pH levels up to 10, retaining full activity after prolonged exposure to flue gas impurities.^[116] Similarly, CA from Thermosynechococcus elongatus exhibits inherent thermostability up to 60°C, with heterologous expression in E. coli yielding enzymes comparable to native forms for high-temperature applications.^[117] Recent advances from 2023-2025 highlight nanomaterials integrated with CA for enhanced CO₂ conversion. Immobilization of CA on ZnO nanoparticles improves thermal stability (optimal pH shifted to 8.5) and reusability, retaining 85% activity over five cycles while catalyzing 476 mg CaCO₃ formation per mg enzyme, accelerating mineralization for industrial scalability.^[118] Zinc single-atom nanozymes mimicking CA achieve over 91% CO₂ conversion efficiency in hydrated systems, outperforming traditional carbon supports by facilitating rapid bicarbonate formation.^[119] Dual-function enzyme systems, such as CA paired with formate dehydrogenase in metal-organic frameworks, enable cascade reactions converting CO₂ directly to formate with high selectivity under ambient conditions.^[120] As of 2025, CA enzymes are increasingly integrated into direct air capture (DAC) systems, enhancing efficiency in low-concentration CO₂ environments.^[121] Novozymes has patented heat-stable CA formulations for potential use in beverage carbonation, utilizing immobilized enzymes in membrane contactors to achieve uniform CO₂ infusion at low energy costs.^[114] These applications underscore CA's versatility beyond natural catalysis, where the enzyme's zinc-histidine coordination drives reversible CO₂ hydration.^[115]

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It can be used with other medications to prevent and control seizures in people with epilepsy. It can also be used to prevent or treat symptoms of altitude ...
[88]
Acetazolamide: Uses, Interactions, Mechanism of Action - DrugBank
Acetazolamide is a carbonic anhydrase inhibitor used to treat edema from heart failure or medications, certain types of epilepsy, and glaucoma.
[89]
Acetazolamide for the prophylaxis of migraine in CADASIL
Feb 25, 2012 · There is no approved treatment for migraine prophylaxis in CADASIL, but acetazolamide has been anecdotally reported to be effective.
[90]
Acetazolamide Efficacy and Tolerability in Migraine With Aura: A ...
Apr 5, 2005 · Acetazolamide has been reported as effective in migraine aura ... migraine patients compared to epileptic subjects. Clarification of ...
[91]
The carbonic anhydrase IX inhibitor SLC-0111 sensitises cancer ...
Here, we demonstrate that the SLC-0111 CAIX inhibitor potentiates cytotoxicity of Dacarbazine and Temozolomide currently used for advanced melanoma treatment.
[92]
Addition of carbonic anhydrase 9 inhibitor SLC-0111 to ... - JCI Insight
Dec 21, 2017 · We determined that SLC-0111 improves the efficacy of temozolomide to extend survival of GBM-bearing mice and should be explored as a treatment strategy.
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A Phase 1 Study of SLC-0111, a Novel Inhibitor of Carbonic...
Depletion of CAIX interferes with pH regulation, reduces cancer stem cells, inhibits epithelial mesenchymal transition and ultimately curtails tumor growth and ...<|separator|>
[94]
The CAIX inhibitor SLC-0111 exerts anti-cancer activity on gastric ...
Sep 1, 2023 · The CAIX inhibitor SLC-0111 exerts anti-cancer activity on gastric cancer cell lines and resensitizes resistant cells to 5-Fluorouracil, taxane-derived, and ...
[95]
Carbonic anhydrase inhibition ameliorates tau toxicity via enhanced ...
Carbonic anhydrase (CA) inhibitors protected against tau toxicity. CRISPR experiments confirmed that CA depletion mimicked the effects of these drugs.
[96]
Articles Sultiame once per day in obstructive sleep apnoea (FLOW)
Oct 31, 2025 · Sultiame is a carbonic anhydrase inhibitor that improves ventilatory response and upper airway muscle tone, two mechanisms known to reduce sleep ...
[97]
Editorial: Emerging advancements in the carbonic anhydrase field
Recent work here reviewed reveals that CA IX blockade unmasks metabolic co-vulnerabilities that sensitize cancer cells to cytotoxic agents by promoting ...
[98]
Dual targeting carbonic anhydrase inhibitors as promising ... - Frontiers
Feb 2, 2025 · The dual-target inhibitor strategy is an evolving approach that holds great potential for treating complex diseases by addressing their multifactorial nature.Missing: polypharmacology | Show results with:polypharmacology
[99]
Carbonic Anhydrase Inhibitors - StatPearls - NCBI Bookshelf
[8] Carbonic anhydrase inhibitor causes alkalization of urine, which is presumed to interact with the excretion of many medications.Indications · Administration · Adverse Effects · Contraindications
[100]
Side effects of acetazolamide: a systematic review and meta ...
Apr 23, 2020 · The most common side effects of AZM are paraesthesias which may reduce therapy ... Reducing side effects of carbonic anhydrase inhibitors.Missing: diuresis | Show results with:diuresis
[101]
Acetazolamide: Considerations for Systemic Administration
Mar 1, 2015 · The diuresis associated with acetazolamide may be temporary due to the high number of carbonic anhydrase enzymes in the kidneys; this may ...
[102]
Carbonic Anhydrase Inhibitors General Statement (Monograph)
Paresthesia, characterized as numbness and a tingling sensation, may occur in the extremities, tongue, and/or the mucocutaneous junctions of the lips or the ...Uses · Dosage · Warnings
[103]
Improving carbon capture utilization and storage (CCUS)
In addition, when CA was added at a concentration of 5.2 μM, a 10-fold increase in CO2 absorption to the uncatalyzed baseline was observed (Hu et al., 2017), ...
[104]
Directed evolution of an ultrastable carbonic anhydrase for highly ...
Nov 3, 2014 · At pilot scale, the evolved catalyst enhanced the rate of CO2 absorption 25-fold compared with the noncatalyzed reaction. Sign up for PNAS ...
[105]
Veolia Uses Enzymes in World-First Trial to Remove CO2 with ...
May 8, 2024 · Veolia aims to reduce the energy consumption, emissions and timescales for carbon removal by utilizing enzymes to accelerate the natural weathering process.
[106]
Discovery of a Highly Thermo- and Alkali-Stable Carbonic Anhydrase
-increase of 0.01-0.03 °C for a value doubling the present concn. of ... Structural mechanics of the pH-dependent activity of β-carbonic anhydrase from.
[107]
[PDF] Global Carbon and Other Biogeochemical Cycles and Feedbacks
In: Global. Warming of 1.5°C. An IPCC Special Report on the impacts of global warming of 1.5°C above pre-industrial levels and related global greenhouse gas.
[108]
Binding Dynamics of Biomimetic Zinc–Cyclen Ionic Liquid Catalyst ...
Sep 30, 2025 · An alternative approach to catalytic hydrolysis of CO2 is presented, using a biomimetic Zn(II)cyclen system formulated with an ionic liquid ...
[109]
Advances in biomimetic carbonic anhydrase strategies for CO2 ...
Jul 1, 2025 · Carbonic anhydrase (CA) enzymes hold strong potential in new biotechnological strategies for accelerated CO2 capture and conversion.
[110]
[PDF] Bifunctional Artificial Carbonic Anhydrase for the Integrated Capture ...
The artificial CA [Zn(cyclen)]2+ increased the rate of capturing CO2 by 84% compared to control experiments and inhibited the HER (partial current density is ...
[111]
A closer look on the development and commercialization of ...
The most studied applications of MC in recent literature are carbon capture and water desalination, while various other applications are getting increasing ...
[112]
WO2010151787A1 - Heat-stable carbonic anhydrases and their use
These membranes are easy to scale-up to industrial scale and have been used in industry for wastewater treatment and beverage carbonation. A schematic ...
[113]
(PDF) Carbonic Anhydrases in Industrial Applications - ResearchGate
Aug 6, 2025 · Due to CA's activity on CO2 there is increasing interest in using CAs for industrial applications such as carbon sequestration and biofuel ...
[114]
Directed evolution of an ultrastable carbonic anhydrase for highly ...
After 9 rounds of evolution, a total of 36 positions representing 15% of the enzyme was mutated (Fig. 4A). Most of the mutations were located at the tetramer ...
[115]
Characterization of heterologously produced carbonic anhydrase ...
The enzyme purified from E. coli has properties (specific activity, inhibitor sensitivity, and thermostability) similar to those of the authentic enzyme ...
[116]
Carbonic Anhydrase Immobilized by ZnO Nanoparticles for Catalytic ...
Aug 6, 2025 · Here, we review the use of carbonic anhydrase nanobiocatalysts for carbon dioxide sequestration, with emphasis on inorganic, organic, and ...
[117]
https://pmc.ncbi.nlm.nih.gov/articles/PMC178080/
[118]
Electroenzymatic CO2 Reduction Using Dual Enzymes ...
May 29, 2025 · Formate dehydrogenase (FDH) and carbonic anhydrase (CA) are co-encapsulated within the Zn-MOF-74 framework to create a dual enzyme cascade system.