Fact-checked by Grok 2 weeks ago

Decidualization

Decidualization is a progesterone-driven differentiation process in which endometrial stromal fibroblasts transform into specialized secretory decidual cells, enabling the human endometrium to support embryo implantation, placentation, and early pregnancy maintenance.^[1] This transformation occurs cyclically during the luteal phase of each menstrual cycle, independent of fertilization, and involves profound morphological and functional changes in the stromal compartment to create a receptive uterine environment.^[2] The process initiates approximately six to eight days after ovulation in perivascular regions near spiral arteries, triggered by rising levels of progesterone and cyclic adenosine monophosphate (cAMP), with estrogen playing a supportive role in priming progesterone receptor expression.^[1] Cellular changes include stromal cell enlargement, accumulation of glycogen and lipids, expansion of the endoplasmic reticulum and Golgi apparatus, and secretion of key markers such as prolactin (PRL) and insulin-like growth factor-binding protein 1 (IGFBP-1).^[1] A network of transcription factors, including HOXA10, FOXO1, and HAND2, orchestrates genetic reprogramming that downregulates pro-inflammatory pathways while upregulating genes for angiogenesis, extracellular matrix remodeling, and immune modulation.^[1] In the absence of pregnancy, declining progesterone leads to spontaneous breakdown of the decidualized endometrium, resulting in menstruation and cyclic regeneration.^[2] Decidualization is essential for pregnancy health, as it facilitates blastocyst attachment, trophoblast invasion, vascular remodeling for placental formation, and maternal immune tolerance toward the semi-allogeneic fetus through recruitment of uterine natural killer (uNK) cells and suppression of excessive inflammation.^[3] It forms three distinct decidual zones—decidua capsularis, parietalis, and basalis—each with specialized functions in nutrient provision and barrier establishment.^[3] Impaired decidualization, often termed "decidualization resistance," is implicated in reproductive disorders, including recurrent pregnancy loss (affecting 1-2% of couples), preeclampsia (5-7% of pregnancies), implantation failure, and preterm birth, highlighting its role as a primary determinant of successful gestation.^[3]

Definition and Overview

Definition and Process

Decidualization refers to the morphological and functional differentiation of endometrial stromal cells (ESCs) into specialized decidual cells, a process essential for establishing pregnancy in humans.^[1] This transformation involves the conversion of fibroblast-like ESCs into large, polygonal epithelioid cells that exhibit proliferation arrest, cytoplasmic expansion, and accumulation of glycogen and lipids.^[4] Key functional changes include the secretion of extracellular matrix proteins, such as prolactin (PRL) and insulin-like growth factor-binding protein-1 (IGFBP-1), which support uterine receptivity and early embryonic development.^[1] In humans, decidualization begins in the mid-secretory phase of the menstrual cycle, approximately 9 days after ovulation (around cycle days 21-25), and progresses through the late secretory phase (up to days 28), independent of embryo implantation.^[2] Unlike in most mammals, where this process is triggered solely by implantation, human decidualization occurs cyclically and spontaneously, leading to menstrual shedding if pregnancy does not ensue.^[4] The initial morphological alterations are evident in perivascular stromal cells surrounding spiral arterioles, with cells adopting a rounded, cobblestone appearance and prominent nuclei.^[5] This process is primarily triggered by rising progesterone levels post-ovulation, often in combination with estrogen and cyclic AMP signaling, though detailed pathways are further explored elsewhere.^[1] Evolutionarily, spontaneous decidualization is a trait unique to higher primates and certain rodents, such as the spiny mouse, facilitating menstruation and precise control over implantation timing.^[6]

Physiological Importance

Decidualization is essential for successful embryo implantation, as it transforms the endometrial stroma into a receptive environment that supports blastocyst attachment and initial trophoblast invasion. This process involves the differentiation of stromal cells into decidual cells, which secrete factors promoting adhesion and nutrient provision to the implanting embryo under hypoxic conditions, thereby ensuring histiotrophic nourishment before placentation.^[4]^[7] Beyond implantation, decidualization protects the maternal endometrium from excessive trophoblast invasion by regulating the modification of spiral arteries through decidual cell secretions, which facilitate controlled vascular remodeling to establish adequate placental blood flow without compromising uterine integrity. This remodeling, initiated by immune cells within the decidua, widens the spiral arteries to support fetal development while preventing over-invasion that could lead to complications.^[4]^[8] Decidualization also facilitates immune tolerance at the maternal-fetal interface by modulating inflammation and promoting the secretion of anti-inflammatory cytokines, such as interleukins and TGF-β, which recruit regulatory immune cells to suppress pro-inflammatory responses and protect the semi-allogeneic embryo from rejection. In the long term, robust decidualization supports successful gestation by maintaining pregnancy viability, reducing the risk of miscarriage through effective embryo quality sensing, and contributing to postpartum endometrial regeneration and hemostasis.^[4]^[7]^[8]

Cellular and Molecular Mechanisms

Hormonal and Signaling Pathways

Decidualization is primarily orchestrated by progesterone, which acts as the master regulator through its nuclear receptors PR-A and PR-B expressed in endometrial stromal cells. PR-A predominantly mediates the anti-proliferative and differentiative effects essential for decidual transformation, while PR-B supports complementary proliferative responses in early stages, ensuring coordinated uterine adaptation for implantation. Progesterone binding to these receptors initiates a cascade that converges with cyclic adenosine monophosphate (cAMP) signaling, elevating intracellular cAMP levels to activate protein kinase A (PKA). This activation leads to phosphorylation of cAMP response element-binding protein (CREB), which in turn drives the transcription of key decidualization markers such as HOXA10 and FOXO1, promoting the genetic reprogramming necessary for stromal cell differentiation.^[9]^[10]^[11] Prior to progesterone dominance, estrogen plays a priming role by enhancing endometrial receptivity, upregulating progesterone receptor expression, and facilitating the transition to a progesterone-responsive state during the mid-luteal phase. This sequential hormonal interplay ensures timely decidualization, with estrogen indirectly supporting progesterone's effects without directly triggering the process. Secondary factors amplify these signals: relaxin boosts cAMP accumulation to potentiate PKA activation, epidermal growth factor (EGF) via its receptor enhances stromal survival and responsiveness, and Wnt signaling, particularly through WNT4, integrates with progesterone to fine-tune morphogen gradients for sustained differentiation. Recent studies highlight metabolic shifts mediated by the AMP-activated protein kinase (AMPK) pathway, where AMPK activation reprograms glycolysis and energy homeostasis to support decidualization resilience, particularly under stress conditions like hyperandrogenism in polycystic ovary syndrome.^[12]^[4]^[13] Feedback mechanisms maintain decidual homeostasis, with decidual prolactin—induced by the cAMP/progesterone axis—acting in an autocrine manner to inhibit excessive stromal proliferation, thereby preventing endometrial hyperplasia and ensuring controlled tissue remodeling. This prolactin-mediated loop dampens pro-inflammatory responses and sustains the differentiated state, linking upstream signaling to long-term uterine stability.^[10]^[14]

Endometrial Stromal Cell Differentiation

Endometrial stromal cells (ESCs), which are fibroblast-like precursors in the uterine lining, undergo profound differentiation during decidualization, transforming into specialized decidual cells essential for pregnancy establishment.^[15] This process is marked by a morphological shift from elongated, spindle-shaped fibroblasts to enlarged, epithelioid-like cells with abundant cytoplasm, accompanied by cell cycle exit into a quiescent G0/G1 arrest state to halt proliferation.^[16] In some mammalian species, including rodents, this differentiation also involves endoreduplication leading to polyploidy in decidual cells, though it does not occur in humans, where differentiation primarily manifests as sustained G0/G1 quiescence.^[17] At the molecular level, decidualization drives specific gene expression changes in ESCs, with upregulation of secretory markers such as prolactin (PRL) and insulin-like growth factor binding protein-1 (IGFBP-1), which support nutrient provision and trophoblast interaction.^[18] Adhesion-related integrins, including α1β1 and α5β1, are also elevated to enhance cell-matrix interactions critical for tissue remodeling.^[19] Conversely, proliferation-associated genes like Ki-67 are downregulated, reinforcing the shift away from cell division toward a differentiated, non-proliferative phenotype.^[20] Functionally, differentiated decidual cells exhibit increased secretory activity, accumulating and releasing factors that foster an implantation-friendly environment, alongside prominent glycogen storage to meet the high energy demands of early pregnancy.^[21] These cells also develop resistance to apoptosis through mechanisms involving oxidative stress adaptation and anti-apoptotic signaling, ensuring their survival during the dynamic implantation phase.^[22] Recent studies highlight heterogeneity in ESC differentiation, particularly through metabolic reprogramming that activates amino acid and sphingolipid pathways to sustain energy production and cellular demands during decidualization.^[23] In vitro, the extent of differentiation is quantitatively assessed via assays measuring PRL secretion into culture media, serving as a reliable index of decidualization efficiency following hormonal stimulation.^[24]

Involvement of Immune Cells and Extracellular Matrix

During decidualization, immune cells infiltrate the endometrium and play essential roles in supporting tissue remodeling and immune tolerance. Uterine natural killer (uNK) cells constitute approximately 70% of the decidual leukocyte population in the first trimester, differentiating from CD34+ progenitor cells and becoming highly abundant at the maternal-fetal interface.^[25] These uNK cells secrete vascular endothelial growth factor (VEGF) and angiopoietins, which promote angiogenesis and vascular remodeling critical for placental development.^[26] Additionally, uNK cells produce cytokines such as interferon-gamma and granulocyte-macrophage colony-stimulating factor, further facilitating tissue homeostasis and trophoblast support.^[27] Macrophages, comprising 10-20% of decidual leukocytes, exhibit regulatory properties that suppress pro-inflammatory responses; for instance, Tim-3+ decidual macrophages induce a Th2 and regulatory T cell (Treg) bias in CD4+ T cells, enhancing immune tolerance.^[28] Tregs, enriched in the decidua, actively suppress effector T cell proliferation and cytokine production, maintaining a tolerogenic environment that prevents fetal rejection.^[29] Extracellular matrix (ECM) reorganization is a hallmark of decidualization, involving dynamic deposition and degradation to accommodate uterine transformation. Recent studies indicate increased deposition of fibronectin, collagen IV, and laminin in the decidual ECM, forming a supportive network that integrates with vascular and stromal components.^[30] These changes, observed in 2025 analyses of bioprinted organoid models, enable structural adaptation during early pregnancy. Matrix metalloproteinases (MMPs), such as MMP-2 and MMP-9, along with their inhibitors (TIMPs), tightly regulate this remodeling by degrading ECM components like collagen IV and fibronectin while preventing excessive breakdown.^[31] This balance ensures controlled tissue expansion and neovascularization without compromising integrity.^[32] Interactions between immune cells, stromal cells, and the ECM are pivotal for coordinated decidualization. Decidual stromal cells recruit uNK cells through secretion of chemokines, including CXCL10 (IP-10), which binds CXCR3 receptors on NK cells to direct their migration and accumulation.^[33] The remodeled ECM serves as a scaffold that modulates trophoblast invasion, providing biomechanical cues and binding sites that limit excessive penetration while supporting attachment and spiral artery remodeling.^[34] For example, laminin and collagen IV in the ECM interact with integrins on trophoblast cells, regulating their motility and ensuring precise implantation depth.^[35]

Decidualization in Reproductive Cycles

In the Menstrual Cycle

Decidualization in the human menstrual cycle begins shortly after ovulation, during the mid-luteal phase, when rising levels of progesterone from the corpus luteum induce differentiation of endometrial stromal cells. This process initiates primarily in perivascular stromal cells surrounding the spiral arteries, which serve as a niche for progenitor cells responsive to progesterone signaling.^[36] From these perivascular sites, decidualization spreads zonally outward through the endometrial stroma, transforming fibroblastic cells into secretory decidual cells characterized by morphological changes such as cytoplasmic expansion and production of extracellular matrix components.^[37] This zonal progression ensures a controlled buildup of the endometrial functional layer, preparing it for potential implantation while occurring independently of embryonic signals.^[10] A distinctive feature of human decidualization is its implantation-independent nature, unlike in rodents where the process is triggered post-implantation by blastocyst-derived signals. In humans, this spontaneous differentiation creates a receptive "window of implantation" around days 20-24 of the cycle, allowing the endometrium to support embryo attachment even in non-conceptive cycles.^[37] Cycle regulation involves estrogen withdrawal following ovulation, which permits progesterone dominance and enhances stromal sensitivity to the hormone. During this phase, HOXA10 expression peaks in the mid-secretory endometrium, acting as a key transcriptional regulator that promotes stromal differentiation and receptivity markers.^[38] In the absence of implantation, the partial decidualization achieved sustains the endometrium temporarily but culminates in shedding upon corpus luteum regression and progesterone decline, typically around day 28. This progesterone withdrawal triggers vasoconstriction of spiral arteries, ischemia, and breakdown of the decidualized layer, resulting in menstruation.^[2] Incomplete or dysregulated decidualization has been associated with conditions like membranous dysmenorrhea, where excessive decidual transformation leads to the expulsion of intact endometrial casts, causing severe cramping and pain during shedding.^[39] The hormonal feedback loop, with rising progesterone initially sustaining the process, ultimately breaks down without pregnancy, ensuring cyclic renewal of the endometrium.^[40]

Preparation for Embryo Implantation

Decidualization profoundly modifies the endometrial stroma to establish a receptive state conducive to blastocyst attachment and initial invasion. This transformation involves the differentiation of stromal fibroblasts into decidual cells, which exhibit enlarged cytoplasm, increased secretory activity, and enhanced intercellular communication, collectively optimizing the uterine environment for pregnancy initiation.^[3] These changes ensure that the endometrium supports the blastocyst's apposition to the luminal epithelium, a critical first step in implantation, while preventing untimely attachments that could compromise reproductive success.^[7] A key adaptation in the receptive endometrium is the expression of adhesion molecules by decidual cells, including L-selectin ligands on the endometrial surface, which interact with L-selectin on the trophoblast to mediate loose apposition and subsequent firm attachment of the blastocyst.^[41] This molecular dialogue, alongside integrins like αvβ3, creates a permissive interface for embryo-endometrium interaction, peaking during the mid-secretory phase. Vascular remodeling accompanies these events, with spiral arteries undergoing coiling and progressive dilation under the influence of decidual factors; uterine natural killer (uNK) cells further drive angiogenesis by secreting vascular endothelial growth factor (VEGF) and other pro-angiogenic signals, ensuring enhanced blood flow and nutrient availability at potential implantation sites.^[7] Decidual cells also adopt a specialized secretory profile, producing prolactin, insulin-like growth factor-binding protein-1 (IGFBP-1), and cytokines such as interleukin-10 (IL-10), which foster an immunosuppressive milieu to shield the implanting embryo from maternal immune surveillance.^[3] This secretory repertoire not only supports local immune tolerance but also modulates extracellular matrix remodeling to facilitate controlled trophoblast invasion. The entire preparatory process is confined to a narrow window of implantation, lasting approximately 4-5 days (typically days 20-24 of the menstrual cycle), during which hormonal cues like progesterone maintain receptivity; any misalignment in this timing disrupts attachment and often results in cycle failure or early pregnancy loss.^[7] In comparative terms, human decidualization initiates pre-implantation in each menstrual cycle, driven primarily by progesterone to proactively ready the endometrium, whereas in mice, it is triggered post-implantation by blastocyst-derived signals, highlighting species-specific strategies for reproductive timing.^[41]

Decidualization During Pregnancy

Role in Placental Development

Decidualization plays a pivotal role in the formation of the placenta by facilitating controlled interactions between maternal decidual cells and fetal trophoblasts, ensuring proper implantation and vascular adaptation in early pregnancy. Following embryo implantation, decidual stromal cells differentiate and secrete factors that regulate extravillous trophoblast (EVT) invasion into the decidua basalis, preventing excessive penetration while promoting necessary remodeling. This process establishes the foundation for nutrient and gas exchange, with decidualization reaching completion around week 8 of gestation, coinciding with peak cytotrophoblast proliferation in chorionic villi to support placental expansion.^[42]^[3] A key mechanism involves decidual cells limiting EVT invasion through secretion of transforming growth factor-β (TGF-β) and tissue inhibitor of metalloproteinases-3 (TIMP-3), which inhibit matrix degradation and trophoblast motility. TGF-β signaling in the decidua activates SMAD3 pathways in EVTs, shifting them from an invasive to a secretory phenotype with increased expression of genes like DAO and PAPPA2, thereby restricting penetration depth to the upper decidua and inner myometrium. Similarly, TIMP-3 expression in decidual and trophoblast cells counteracts matrix metalloproteinases (MMPs), fine-tuning invasion to avoid pathological overreach. These regulatory actions ensure balanced trophoblast activity essential for placental anchoring without disrupting maternal tissue integrity.^[43]^[44] Decidualization also drives spiral artery remodeling via uterine natural killer (uNK) cells and decidual macrophages, which infiltrate vessels and induce endothelial apoptosis and extracellular matrix breakdown to create low-resistance conduits for maternal blood flow to the placenta. uNK cells secrete angiogenic factors such as vascular endothelial growth factor (VEGF) and placental growth factor (PlGF), while both cell types produce MMP-7 and MMP-9 to degrade vessel walls, enabling EVT-mediated replacement of smooth muscle with fibrinoid deposits. This remodeling, initiated in the first trimester, transforms high-resistance spiral arteries into dilated, low-resistance vessels by week 8, optimizing uteroplacental perfusion for fetal development.^[45]^[46] The decidual extracellular matrix (ECM) further contributes by integrating with syncytiotrophoblast layers in chorionic villi, anchoring the placenta and facilitating nutrient exchange across the maternal-fetal interface. Decidual fibroblasts, post-decidualization, deposit ECM components like fibronectin and laminin that form stable attachments with anchoring villi, supporting cytotrophoblast proliferation and villous expansion for efficient diffusion of oxygen and nutrients. Recent studies highlight how this ECM scaffold, enriched by decidual secretions, maintains barrier integrity while allowing selective trophoblast egress.^[47] Emerging 2025 research on crosstalk between decidual NK (dNK) cells and EVTs underscores HLA-G-mediated regulation of invasion, where HLA-G on EVTs binds KIR2DL4 on dNKs to activate NF-κB signaling, promoting secretion of pro-invasive cytokines like IL-8 and IP-10. This interaction balances immune tolerance with controlled trophoblast motility, preventing rejection while ensuring adequate placental invasion; disruptions in this pathway correlate with implantation failures. dNK subsets, particularly those expressing high KIR2DL1/2/3, fine-tune these responses to support early placental morphogenesis.^[48]

Maintenance of Gestation and Immune Tolerance

Decidualization plays a crucial role in establishing immune privilege at the maternal-fetal interface, where regulatory T cells (Tregs) in the decidua suppress alloreactive immune responses to prevent fetal rejection. Decidual Tregs express inhibitory molecules such as CTLA-4, CD25, and PD-L1, while secreting anti-inflammatory cytokines like IL-10 and TGF-β to constrain effector T cell activity and maintain tolerance toward paternal antigens expressed by the fetus.^[49]^[50] IL-10, produced by decidual cells and trophoblasts, further dampens pro-inflammatory responses by promoting Treg homeostasis and inhibiting Th1/Th17-mediated immunity, ensuring a tolerogenic environment throughout gestation.^[51] Hormonal signals from the trophoblast sustain decidualization by maintaining elevated progesterone levels essential for gestational stability. Human chorionic gonadotropin (hCG), secreted by syncytiotrophoblasts, stimulates the corpus luteum to produce progesterone, which in turn supports ongoing differentiation and secretory functions of decidual stromal cells.^[52]^[53] This feedback loop prevents luteolysis and sustains the decidual phenotype, providing metabolic and structural support for the developing placenta.^[54] Decidual cells contribute to structural integrity by forming a mechanical barrier that shields the fetus from ascending infections, augmented by the production of antimicrobial peptides such as β-defensins. These peptides, including human β-defensin-1 and -2 expressed in decidual and placental tissues, exhibit broad-spectrum activity against bacteria, viruses, and fungi, enhancing innate defense at the interface.^[55]^[56] The robust extracellular matrix secreted by decidual cells further reinforces this barrier, limiting pathogen invasion while permitting controlled trophoblast migration.^[57] In late gestation, decidual tissue undergoes gradual regression following placental formation, orchestrated by senescence pathways that ensure timely breakdown and initiation of labor. Cellular senescence in decidual stromal cells triggers the senescence-associated secretory phenotype (SASP), releasing pro-inflammatory factors that promote tissue remodeling without compromising mid-gestation stability.^[58] Recent 2025 reviews highlight how p53/p21 and p16/Rb pathways regulate this senescence, facilitating decidual involution and preventing prolonged pregnancy complications.^[59]^[60] Decidual cells manage oxidative stress from placental metabolism through activation of NRF2 and autophagy pathways, protecting against reactive oxygen species (ROS)-induced damage. NRF2 transcriptionally upregulates antioxidant genes in response to ROS, mitigating lipid peroxidation and apoptosis in decidual tissue during heightened metabolic demands.^[61] Autophagy, induced in decidual stromal cells, clears damaged organelles and maintains redox balance, supporting cell survival and functional integrity under oxidative pressure.^[62]^[22]

Pathophysiological Implications

In Infertility and Implantation Disorders

Infertility affects approximately 10-15% of couples worldwide, with decidualization defects implicated in a substantial portion of cases, particularly unexplained infertility and recurrent implantation failure (RIF).^[63] These defects disrupt the endometrial stromal cell transformation necessary for embryo implantation. Dysregulation in key pathways, including those involving FOXO1 and progesterone receptor (PR), contributes to impaired decidualization and associated infertility.^[64] For instance, dysregulation of FOXO1, a critical regulator of progesterone-dependent endometrial differentiation, correlates with reduced endometrial receptivity and pregnancy establishment.^[64] Anomalies in PR signaling pathways hinder the hormonal cues required for proper stromal responsiveness.^[65] In unexplained infertility, impaired decidualization manifests as reduced secretion of prolactin (PRL) and insulin-like growth factor-binding protein 1 (IGFBP-1), key markers of stromal differentiation. Studies of endometrial stromal cells from women with endometriosis show significantly lower PRL and IGFBP-1 levels during in vitro decidualization compared to fertile controls, indicating defective responsiveness to progesterone.^[66] This impairment is observed in up to 62.5% of cases in some cohorts of infertile women, highlighting its prevalence in otherwise unexplained infertility where no structural or ovulatory issues are evident.^[67] The reduced secretion disrupts the secretory and supportive environment needed for implantation, contributing to cycles of failed conception. Recurrent implantation failure (RIF), defined as the absence of pregnancy after multiple embryo transfers in assisted reproduction, often stems from defective endometrial stromal responsiveness to progesterone during decidualization. Functional impairment of HOXA10, such as through enhanced sumoylation, is associated with RIF, leading to altered expression of implantation-related genes.^[68] For example, enhanced sumoylation of HOXA10 inhibits decidualization and embryo attachment in vitro, directly linking this molecular defect to implantation incompetence.^[68] Additionally, progesterone resistance in the endometrium exacerbates these issues, impairing the window of implantation and resulting in repeated failures despite viable embryos.^[69] Defects in decidualization also contribute to early miscarriage through inadequate vascular remodeling and poor placentation. Insufficient stromal differentiation fails to support the invasion of trophoblast cells and spiral artery transformation, compromising nutrient supply to the developing embryo. Recent studies, including those from 2024, have linked these defects to oxidative stress imbalances in decidual cells, where elevated reactive oxygen species cause mitochondrial damage and reduced cell survival, predisposing to spontaneous abortion.^[70] In particular, stalled or weakened decidual reactions correlate more strongly with miscarriage recurrence than other factors like uterine natural killer cell expansion.^[59] Diagnostic approaches increasingly rely on in vitro decidualization assays to predict IVF success, offering a functional assessment of endometrial competence. These assays measure PRL and IGFBP-1 secretion from patient-derived stromal cells exposed to progesterone and cAMP, with lower outputs indicating decidualization defects and poorer implantation outcomes.^[71] Such markers have shown promise in identifying women at risk for RIF or miscarriage, enabling personalized IVF strategies, though broader validation is ongoing.^[72]

Associations with Endometriosis and Other Gynecological Conditions

In endometriosis, the eutopic endometrium exhibits progesterone resistance, which disrupts the normal progesterone-dependent signaling required for effective decidualization of endometrial stromal cells.^[65] This resistance is associated with impaired expression of key transcription factors such as FOXO1 and HOXA10, which are essential for stromal cell differentiation and receptivity during the implantation window.^[73] Studies have shown that reduced HOXA10 levels in the eutopic endometrium of women with endometriosis correlate with defective decidualization markers, including prolactin and IGFBP-1 secretion. In polycystic ovary syndrome (PCOS), hyperandrogenism directly inhibits endometrial stromal cell differentiation by interfering with progesterone-responsive pathways, leading to suboptimal decidualization.^[13] Elevated androgens, such as dihydrotestosterone, alter the expression of decidualization regulators like PDK4, resulting in reduced stromal responsiveness to hormonal cues.^[13] This impaired process contributes to a characteristically thin endometrium, which compromises implantation success and increases the risk of early pregnancy loss.^[74] Adenomyosis impairs decidualization through molecular pathway dysregulation, leading to incomplete decidual transformation and reduced endometrial receptivity.^[75]^[76] Similarly, uterine fibroids (leiomyomas) impair vascular changes by promoting local hypoxia and dysregulated angiogenesis, which affect spiral artery remodeling and nutrient delivery to the implantation site.^[77] Elevated vascular endothelial growth factor (VEGF) levels in fibroid tissues further exacerbate these disruptions, altering the vascular permeability required for healthy decidual progression.^[78] At the molecular level, epigenetic silencing of decidualization-related genes, such as those encoding HOXA10, occurs through hypermethylation of promoter regions in endometriotic endometrium, suppressing progesterone-induced gene expression.^[79] Inflammatory cytokines, particularly IL-6, further impair the process by promoting a pro-inflammatory microenvironment that inhibits stromal cell differentiation.^[80] This cytokine-driven inflammation overrides progesterone effects, leading to persistent activation of NF-κB pathways and reduced expression of decidual markers.^[80] Clinically, decidualization defects in endometriosis elevate the risk of ectopic pregnancy by compromising the endometrium's ability to support proper embryo attachment and trophoblast invasion.^[81] Additionally, accelerated cellular senescence in the decidua, driven by oxidative stress and inflammatory signals like IL-1β, contributes to premature stromal cell arrest and impaired gestation maintenance.^[82] This senescence is linked to upregulated p16 and p21 expression, further exacerbating implantation failures and adverse pregnancy outcomes.^[83] As of 2025, emerging research highlights potential therapeutic targets, such as modulating oxidative stress pathways in decidual cells to mitigate recurrent spontaneous abortion risks associated with these defects.^[70]

Current Research and Models

In Vitro and Animal Models

In vitro models of decidualization primarily utilize primary human endometrial stromal cells (ESCs) isolated from biopsy samples and cultured in the presence of medroxyprogesterone acetate (MPA) combined with cyclic adenosine monophosphate (cAMP) analogs, such as 8-bromo-cAMP, to mimic progesterone-driven differentiation over 8–14 days.^[18]^[84] These conditions induce characteristic morphological changes, including cell enlargement and prolactin secretion, allowing researchers to study decidual markers like IGFBP1 and FOXM1 without the need for implantation cues.^[85]^[86] To quantify decidualization progression, 2023 protocols employ high-content imaging and automated analysis of cellular morphology, such as area, circularity, and granularity, enabling early detection of phenotypic shifts as soon as 2 days post-induction.^[87]^[88] These image-based methods provide objective metrics for evaluating treatment effects, surpassing traditional biochemical assays in sensitivity and throughput.^[89] Advancements in three-dimensional (3D) models include endometrial organoids derived from primary ESCs or stem cells, which incorporate extracellular matrix (ECM) components like Matrigel to recapitulate stromal architecture and facilitate immune interactions.^[90]^[91] Co-cultures of decidual organoids with trophoblast organoids simulate maternal-fetal interfaces, revealing reduced viral susceptibility in co-cultured decidua due to trophoblast-derived signals that enhance innate immune responses.^[92]^[93] Animal models, particularly in mice, rely on pseudopregnancy induced by mating with vasectomized males or progesterone administration to trigger decidualization, often via artificial stimuli like oil injection to mimic implantation.^[94]^[95] However, mouse decidualization is strictly implantation-dependent and lacks the spontaneous progesterone-initiated process seen in humans during the menstrual cycle.^[96]^[37] Key limitations of rodent models include the absence of a true menstrual cycle and spontaneous decidualization, which restricts their applicability to human reproductive disorders involving pre-implantation endometrial changes.^[97] Emerging human induced pluripotent stem cell (iPSC)-derived ESCs offer a solution by enabling personalized studies of decidualization in patient-specific genetic contexts, bypassing ethical and availability issues with primary tissues.^[97] Recent advances in 2024 metabolic profiling of in vitro decidualized ESCs have highlighted upregulated sphingolipid metabolism, including ceramide and sphingosine-1-phosphate pathways, as critical for stromal cell differentiation and survival.^[98] These findings underscore sphingolipids' role in modulating decidual plasticity, with inhibitors disrupting prolactin expression and cell morphology.^[99]

Emerging Therapeutic Targets and Future Directions

Recent research has identified several molecular targets for enhancing decidualization in cases of progesterone resistance, particularly in conditions like endometriosis. Histone-modifying enzymes, such as those involved in epigenetic regulation, have emerged as potential therapeutic avenues to improve decidualization in eutopic endometrium from women with endometriosis by counteracting impaired stromal cell differentiation.^[100] Additionally, modulation of the SUMOylation pathway has been shown to sensitize endometrial stromal cells to progesterone signaling, promoting robust decidualization during hormone-induced differentiation.^[101] FOXO1, a critical transcription factor in decidualization, has been targeted through upstream regulators like SOX4, which stabilizes progesterone receptor and enhances FOXO1 expression to drive stromal cell transformation; agonists or mimetics of FOXO1 signaling pathways hold promise for augmenting differentiation in resistant cases.^[102] Senescence modulation represents a promising strategy to address defective decidualization, with 2025 studies demonstrating that flavonoid senolytics—such as quercetin, fisetin, and luteolin—effectively clear senescent endometrial stromal cells, thereby enhancing the decidualization capacity of remaining cells and balancing autophagy-related pathways in the decidua.^[103] These agents remove senescence-associated secretory phenotype (SASP) burdens that impair differentiation, potentially restoring endometrial receptivity in infertility contexts.^[104] Metabolic interventions targeting energy homeostasis are also under investigation. AMPK activators, including metformin and resveratrol, have been shown to mitigate oxidative stress in decidual cells by promoting sestrin 2/AMPK/mTORC1 signaling, thereby supporting uterine receptivity and reducing risks of preterm birth in preclinical models.^[105] In decidual defects, inhibitors of dysregulated sphingolipid synthesis, such as serine palmitoyltransferase (SPT) blockers, may normalize metabolic flux, as excessive de novo sphingolipid production disrupts stromal differentiation while controlled inhibition preserves essential pathways for implantation.^[106] Future directions emphasize advanced profiling techniques to uncover decidual heterogeneity and improve clinical outcomes. Single-cell RNA sequencing (scRNA-seq) has revealed dynamic transcriptional landscapes during decidualization, highlighting stromal cell subpopulations and their metabolic reprogramming, which could guide personalized therapies for implantation failures.^[107] AI-driven models are emerging to predict decidualization success in IVF by analyzing endometrial imaging and molecular data, achieving high accuracy in predictions for receptivity and pregnancy outcomes.^[108] As of 2025, ongoing efforts include validation of prolactin (PRL)-based biomarkers for assessing decidualization status in recurrent implantation failure, leveraging PRL's role as a key secretory marker to stratify patients.^[109] Gene therapy approaches targeting epigenetic defects, such as those involving HOXA11 or histone modifications, show potential for correcting genetic disruptions in decidualization, though clinical translation remains exploratory.^[110]

References

[1]
Decidualization of the human endometrium - PMC - PubMed Central
Decidualization of the human endometrium, which involves a dramatic morphological and functional differentiation of human endometrial stromal cells (ESCs),
[2]
Cyclic Decidualization of the Human Endometrium in Reproductive ...
In response to falling progesterone levels, spontaneous decidualization causes menstrual shedding and cyclic regeneration of the endometrium.
[3]
Endometrial Decidualization: The Primary Driver of Pregnancy Health
Jun 8, 2020 · The process of implantation/placentation results in the formation of three decidual regions, which are anatomically and functionally distinct.
[4]
Endometrial Decidualization: The Primary Driver of Pregnancy Health
Jun 8, 2020 · Decidualization refers to the functional and morphological changes that occur within the endometrium to form the decidual lining into which the ...3. Implantation And... · 5. The Decidua As An... · 6. Endometrial...<|control11|><|separator|>
[5]
Morphophysical dynamics of human endometrial cells during ...
A defining feature of decidualization is the morphological change induced in ESCs, which includes the acquisition of a rounded shape, cobblestone morphology ...
[6]
The Role of Decidual Subpopulations in Implantation, Menstruation ...
... mammals, including higher primates and humans (21, 22). Further, emergence of menstruation in the primate lineage occurred in parallel with genomic ...
[7]
Physiologic Events of Embryo Implantation and Decidualization in ...
Mar 13, 2020 · The focus of this review is to provide a general concept of the mechanisms involved during the window of implantation, description of components ...
[8]
Decidualization and Related Pregnancy Complications
Insufficient decidualization leads to abnormal implantation/placentation and ultimately results in adverse pregnancy outcomes.
[9]
The progesterone receptor regulates implantation, decidualization ...
This review highlights the integral role of uterine progesterone-mediated paracrine signaling in gland development and pregnancy.
[10]
Decidualization of the human endometrium: mechanisms ... - PubMed
This differentiation process is dependent entirely on the convergence of the cyclic adenosine monophosphate and progesterone signaling pathways that drives ...
[11]
The role of FOXO1 in the decidual transformation of the ... - PubMed
FOXO1 is of particular importance as a critical regulator of progesterone-dependent differentiation, menstrual shedding, and protection of the feto-maternal.
[12]
Progress on the Role of Estrogen and Progesterone Signaling in ...
Jan 24, 2023 · This article reviews the recent advances in the molecular mechanisms of estrogen- and progesterone-regulating uterine receptivity establishment, blastocyst ...
[13]
Decreased AMPK/SIRT1/PDK4 induced by androgen excess inhibits ...
Jul 30, 2024 · Androgen excess in the endometrium inhibits decidualization by disrupting the AMPK/SIRT1/PDK4 signaling pathway. These data demonstrate the ...
[14]
Immunomodulatory role of decidual prolactin on the human fetal ...
Jun 9, 2023 · (1) PRL inhibits decidual production of IL-6, maintains P4 production, regulates endometrial vascularization, and stimulates proliferation of ...
[15]
The Regulators of Human Endometrial Stromal Cell Decidualization
The decidualization process is characterized by changes in human endometrial stromal cell (hESC) morphology, increase in uterine gland secretion, elevation of ...
[16]
Cell Cycle Regulation of Human Endometrial Stromal Cells During ...
Our data imply that the decidualization of HESC is associated with cell cycle arrest at G0/G1 phase initially and G2/M phase at later stages. Our results also ...Methods · Results · Conclusions
[17]
Involvement of atypical transcription factor E2F8 in the ... - PubMed
E2F8-mediated polyploidization is a response to stresses which are accompanied by decidualization. Interestingly, polyploidization is not detected during human ...
[18]
Differential gene expression in decidualized human endometrial ...
Apr 2, 2024 · As an alternative to MPA, cAMP has been widely used as a decidualization stimulus because cAMP is a second messenger of progesterone and can ...
[19]
The characteristics of integrins expression in decidualized human ...
Integrins, a class of cell adhesion molecules, are known to be involved in cell-cell and cell-substrate adhesion, and suggested to play an important role in ...
[20]
Decreased PIBF1/IL6/p-STAT3 during the mid-secretory phase ...
PIBF1 regulated HESC proliferation and decidualization via IL6/p-STAT3 signaling. •. The IL6/p-STAT3, Ki-67, prolactin, and IGFBP1 levels were lower in RIF ...
[21]
Mitochondria remodeling during endometrial stromal cell ...
Oct 4, 2024 · This step, known as decidualization, is crucial for embryo implantation. We previously demonstrated how the secretory pathway is remodelled ...
[22]
Mechanisms driving resistance to oxidative stress during ...
Decidualization is the transformation of endometrial stromal cells into functionally specialized cells during the early stages of pregnancy.
[23]
Metabolic reprogramming and heterogeneity during the ...
Jul 30, 2024 · The human endometrial decidualization is a transformative event in the pregnant uterus that involves the differentiation of stromal cells ...
[24]
Activation of the Decidual Prolactin Gene - PubMed
Decidualization of human endometrial stromal (ES) cells in vitro is induced by cAMP analogues and ligands that elevate cellular cAMP levels in a manner ...
[25]
Role of uterine NK cells in pregnancy complication - ScienceDirect
This review focuses on the role of uNK cells in pregnancy disorders such as preeclampsia, recurrent pregnancy loss, and endometriosis.Review · Abstract · Introduction
[26]
Cytokine modulation and immunoregulation of uterine NK cells in ...
Immune cells, including uNK cells, T cells, macrophages, and DCs, play critical roles in endometrial receptivity. These cells are involved in processes such as ...
[27]
Natural cytotoxicity receptor 1 in mouse uNK cell maturation and ...
Jan 18, 2017 · NKp46 cross-linking of first trimester human uNK cells also induces dose-dependent production of cytokines that include VEGF and placental ...
[28]
Tim-3+ decidual Mφs induced Th2 and Treg bias in ... - Nature
May 12, 2022 · With higher CD132 expression, Tim-3 + dMφs subset induced Th2 and Treg bias in decidual CD4 + T cells and promoted pregnancy maintenance.
[29]
Decidual stromal cells support tolerance at the human foetal ...
Macrophages with regulatory properties and regulatory T (Treg) cells are central in promoting foetal tolerance and are enriched in the decidua (the uterine ...
[30]
Matrix directs trophoblast differentiation in a bioprinted organoid ...
Sep 12, 2025 · Given the critical role of the extracellular matrix (ECM) in guiding cell behaviour, including differentiation, organisation and function, there ...
[31]
Expression of matrix metalloproteinases and tissue inhibitors of ...
These results suggest that MMP-2 may participate in the early phase of decidualization and neovascularization required for placentation. The restricted MMP-9 ...
[32]
Matrix metalloproteinases and their inhibitors in vascular remodeling ...
Jan 15, 2008 · The balance between MMPs and TIMPs plays a major role in vascular remodeling, angiogenesis, and the uterine and systemic vasodilation during normal pregnancy.
[33]
Decidual Cell Regulation of Natural Killer Cell–Recruiting ...
Decidual cell–mediated recruitment of NK cells in normal pregnancy is indicated by a report that progesterone enhances mRNA expression of NK cell-recruiting ...
[34]
Decidualization - an overview | ScienceDirect Topics
This process of decidualization, occurs during the late secretory phase of the menstrual cycle (around day 22, or 6 days prior to potential blastocyst ...Missing: starts | Show results with:starts
[35]
Collagen type IV at the fetal–maternal interface - ScienceDirect.com
Col-IV is expressed as novel forms in the placenta. These findings suggest that col-IV not only represents a structural protein providing tissue integrity ...
[36]
The mechanism of Shoutai Wan in the treatment of recurrent ...
Jun 30, 2024 · Activation of the Nrf2/HO-1 pathway enhances cellular antioxidant capabilities, reduces reactive oxygen species and free radical accumulation, ...
[37]
Modeling the human maternal-fetal interface - ScienceDirect.com
Sep 4, 2025 · NOTCH signaling regulates key aspects of human placental development by controlling trophoblast proliferation, differentiation, and invasion.
[38]
Unveiling uterine aging: Much more to learn - ScienceDirect
We summarize the most recent findings regarding uterine aging, including functional and structural alterations and potential cellular and molecular mechanisms.
[39]
Decidualization Induces a Secretome Switch in Perivascular Niche ...
Decidualization, a process characterized by the transformation of stromal fibroblast into specialized secretory decidual cells, is driven by the postovulatory ...Missing: initiation | Show results with:initiation
[40]
ENDOMETRIAL DECIDUALIZATION: OF MICE AND MEN - PMC
Although the developmental changes that define various stages of embryo implantation and decidualization have been described in both mice and humans, the ...
[41]
Regulation of HOXA-10 and its expression in normal and abnormal ...
HOXA-10 appears to increase in the early secretory phase with peak expression during the mid-secretory phase, concomitant with maximal expression of integrin β3 ...
[42]
Unusual Manifestation of Membranous Dysmenorrhea: Case Report
This causes intense and painful contractions of the myometrium, aggravated by the expulsion of tissues produced by the decidualization of the endometrium.
[43]
Molecular and cellular mechanisms for differentiation and ... - PubMed
The postovulatory rise in ovarian progesterone induces profound remodeling and differentiation of the estradiol-primed endometrium. This change, termed ...
[44]
Mechanisms of implantation: strategies for successful pregnancy - NIH
The importance of pre-decidualization is perhaps to prepare the endometrium for implantation and seems analogous to extensive stromal cell proliferation with ...
[45]
Decidualization resistance in the origin of preeclampsia - PubMed
Decidualization resistance, which is a defective endometrial cell differentiation during the menstrual cycle, could underlie shallow trophoblast invasion.
[46]
Transforming growth factor-β signaling governs the differentiation ...
Jul 6, 2022 · In the decidua, iEVTs up-regulate TGF-β receptors and TGF-β signaling, thereby slowing down migration and promoting differentiation into a ...
[47]
Induction of tissue inhibitor of metalloproteinase 3 gene expression ...
... TIMP-3 expression in decidual cells and trophoblasts may be important in the regulation of trophoblast invasion. Publication types. Research Support, Non ...
[48]
Mechanisms of Human Decidual Spiral Artery Remodeling in Vitro
We hypothesized that uNK cells and macrophages participate in artery remodeling through specific mechanisms inducing vascular cell priming, destruction, and ...
[49]
Decidual macrophages: key regulators of vascular remodeling in ...
Jan 27, 2016 · It has been proposed that uNK cells play a role in the EVT-independent phase of spiral artery remodeling, as they secrete a wide range of ...
[50]
Extracellular matrix and pregnancy: functions and opportunities ...
Feb 14, 2025 · The decidua contributes to early nutrient exchange, production of cytokines, and growth factors as well as supporting the development of new ...
[51]
Crosstalk between decidual natural killer cells and extravillous ...
Oct 22, 2025 · The interaction between HLA-G on EVTs and KIR2DL4 on dNKs activates the NF-κB signaling pathway in dNKs, promoting the secretion of interlukine- ...
[52]
Beyond Immune Balance: The Pivotal Role of Decidual Regulatory T ...
May 1, 2024 · Decidual Treg cells may contribute to constraining Teffs in early pregnancy by expressing CTLA4, CD25, and PD-L1, and secreting TGF-β as well as ...
[53]
Regulatory T-cells and immune tolerance in pregnancy: a new target ...
Collectively, these studies support the notion that Treg cells are required to suppress maternal immune responses targeted against fetal alloantigens, rather ...
[54]
Interleukin-10: A Multi-Faceted Agent of Pregnancy - PMC
This review focuses on the role of IL-10 as a facilitator of successful pregnancy both as an immune suppressive agent and mediator of cross-talk between the ...Trophoblasts · Unk Cells And Monocytes · T Regulatory Cells<|separator|>
[55]
Human trophoblast function during the implantation process
Oct 20, 2005 · Hormones such as hCG, produced by the trophoblast, maintain this progesterone production in early pregnancy [71]. Progesterone was suggested ...
[56]
hCG: Biological Functions and Clinical Applications - MDPI
During pregnancy, syncytiotrophoblast derived hCG also induces corpus luteal cells to produce progesterone, whereas cytotrophoblast-derived hCG-H appears to act ...
[57]
[PDF] PLACENTAL HORMONES - Yale University
Nov 3, 1993 · Early in gestation, human chorionic gonadotropin (hCG) is essential to maintain corpus luteum progesterone production. Near the end of the first ...
[58]
Tissue-specific human beta-defensins (HBD)1, HBD2, and HBD3 ...
Dec 1, 2010 · Differential secretion of antimicrobial peptides is a key mechanism in the innate immunity to respond efectively and quickly to control and ...
[59]
Defensins: defenders of human reproductive health - Oxford Academic
Among various antimicrobial peptides expressed in the human body, defensins are one of the most abundantly expressed classes. They are a class of cationic ...Missing: mechanical | Show results with:mechanical
[60]
The decidua—the maternal bed embracing the embryo—maintains ...
Jun 10, 2016 · During decidualization, vascular and tissue remodeling in the uterus provide nutritional and physical support for the developing embryo.<|separator|>
[61]
Role of Cellular Senescence in IUGR: Impact on Fetal Morbidity and ...
Jul 17, 2025 · Studies indicate that decidual senescence and the associated inflammation through the SASP play a significant role in spontaneous labor. ...Missing: regression | Show results with:regression
[62]
Stalling of the endometrial decidual reaction determines ... - Science
Jun 25, 2025 · Our findings show that erosion of the decidual reaction following a miscarriage drives the recurrence risk irrespective of maternal age.Missing: late | Show results with:late
[63]
Molecular pathways of senescence regulate placental structure and ...
Aug 19, 2019 · We propose that molecular mediators of senescence regulate placental structure and function, through both cell‐autonomous and non‐autonomous mechanisms.Missing: regression | Show results with:regression
[64]
Divergent Regulation of Decidual Oxidative-Stress Response by ...
Feb 10, 2022 · Divergent regulation of decidual oxidative-stress response by NRF2 and KEAP1 in preeclampsia with and without fetal growth restriction.
[65]
The regulated cell death at the maternal-fetal interface - Nature
Feb 26, 2024 · In this review, we summarized the apoptosis, necroptosis and autophagy play both promoting and inhibiting roles in the differentiation, invasion ...
[66]
Mechanisms of Decidual Dysfunction and Infertility in Endometriosis
Jun 19, 2025 · Dysregulation of the HAND2‐NR2F2 axis, akin to HOXA10 and FOXO1 anomalies, can impair endometrial receptivity and contribute to infertility.Missing: couples variants
[67]
a new investigation into the role of eutopic endometrium | Human ...
Our results demonstrated a reduced decidualization capacity for eutopic endometriotic ESC, since PRL and IGFBP-1 levels released were significantly lower with ...Missing: correlation | Show results with:correlation
[68]
High incidence of decidualization failure in infertile women - NIH
May 16, 2024 · 20 cases (62.5%) showed decidualization failure. These patients tended to have a history of more frequent miscarriages than those without decidualization ...Missing: couples variants
[69]
Enhanced HOXA10 sumoylation inhibits embryo implantation in ...
Oct 9, 2017 · Our results suggest that HOXA10 sumoylation impairs the process of embryo implantation in vitro and takes part in the development of RIF.
[70]
FHL1 mediates HOXA10 deacetylation via SIRT2 to enhance ...
Nov 22, 2022 · Recurrent implantation failure (RIF) is defined as the failure of clinical pregnancy in a woman younger than 40 years following the transfer ...<|separator|>
[71]
Evaluation of Progesterone Resistance and the Role of ... - medRxiv
Feb 24, 2025 · This study investigates the impact of progesterone resistance on endometrial receptivity and evaluates the efficacy of pituitary downregulation ...
[72]
Oxidative stress-induced decreased expression of FABP5 leads to ...
Oxidative stress-induced decreased expression of FABP5 leads to mitochondrial damage and survival disorder of decidual stromal cells in women with recurrent ...
[73]
Impaired decidualization of human endometrial stromal cells from ...
Oct 26, 2020 · We found that the expression of decidualization markers (IGFBP1 and PRL) in ESCs of adenomyosis was reduced, concomitant with increased cell ...
[74]
An IGF1-expressing endometrial stromal cell population is ...
Dec 8, 2022 · Decidualization refers to the process of transformation of endometrial stromal fibroblast cells into specialized decidual stromal cells that ...
[75]
Progesterone Resistance in Endometriosis: Current Evidence and ...
Apr 10, 2023 · In this review, we aim to summarize the evidence supporting progesterone resistance in endometriosis and the currently proposed mechanisms.
[76]
Mechanisms of Decidual Dysfunction and Infertility in Endometriosis ...
Jun 19, 2025 · The cAMP signaling pathway increases COX2 expression in human endometrial epithelial cells [61, 62]. During early pregnancy, cytosolic ...
[77]
Decidualized endometrial stromal cells present with altered ... - Nature
Aug 11, 2021 · Hyperandrogenic women with PCOS show disrupted decidualization (DE) and placentation. Dihydrotestosterone (DHT) is reported to enhance DE in ...
[78]
Endometrial stromal fibroblasts from women with polycystic ovary ...
Endometrial stromal fibroblasts from women with polycystic ovary syndrome have impaired progesterone-mediated decidualization, aberrant cytokine profiles and ...
[79]
Molecular mechanism of aberrant decidualization in adenomyosis ...
Jan 16, 2025 · The molecular mechanism by which aberrant endometrial decidualization in adenomyosis causes a reduction in endometrial receptivity is clarified ...
[80]
Decreased Krüppel-like factor 4 in adenomyosis impairs ...
Jun 27, 2022 · Poor decidualization and abnormal autophagy conditions in the endometria of adenomyosis patients have been reported previously.
[81]
Vascular Biology of Uterine Fibroids - PubMed Central
Vascular dysfunction in uterine fibroids may create a local hypoxic milieu that upregulates HIF-1α, and in turn, ET-1 expression. In the fibroid cell, ET-1 ...
[82]
Uterine Fibroids (Leiomyomata) and Heavy Menstrual Bleeding
In uterine fibroids, VEGF levels have been reported as either similar (70) or increased (71) in fibroid tissues vs. adjacent myometrium, with an observation of ...<|separator|>
[83]
Systematic review on the DNA methylation role in endometriosis
Feb 21, 2025 · Our systematic review indicates that endometriosis is associated with DNA methylation modifications at specific genes involved in key endometrial biological ...
[84]
Endometrial Inflammation and Impaired Spontaneous Decidualization
Specifically, the adenomyotic lesion environment comprises inflammatory mediators, including proinflammatory cytokines (e.g., IL-6, IL-1β, IL-8), anti- ...
[85]
Endometriosis and the Role of Pro-Inflammatory and Anti ... - NIH
Jan 31, 2024 · Dysbiosis of the endometrial microbiota and pro-inflammatory cytokines such as IL-6, IL-8, and IL-17 are implicated in infertility and in cancer ...
[86]
Interleukin-1β induces and accelerates human endometrial stromal ...
Jun 15, 2024 · The results support the hypotheses that “inflammaging” contributes to endometrial senescence, disruption of decidualization and impairment of fecundity.
[87]
CDC42 deficiency leads to endometrial stromal cell senescence in ...
Nov 1, 2024 · In particular, decidualization defects and implantation failure have been linked to the enhanced level of premature senescence of EnSCs during ...
[88]
Isolation of Human Endometrial Stromal Cells for In Vitro ... - NIH
Sep 1, 2018 · This study provides a detailed protocol for the isolation of HESC and subsequent artificial decidualization via the supplementation of hormones to the ...
[89]
Progesterone and cAMP synergistically induce SHP2 expression via ...
Oct 3, 2023 · Estrogen, progesterone and cAMP co-induced decidualization of human endometrial stromal cell in vitro, but only progesterone and cAMP induced ...
[90]
METTL3-regulated m6A modification impairs the decidualization of ...
Oct 27, 2023 · Additionally, we treated normal primary stromal cells with MPA and 8-Br-cAMP for in vitro decidualization and found that METTL3 expression ...
[91]
Quantitative Analysis of Cellular Morphology During In Vitro ...
Oct 9, 2023 · A simple and accurate methodology was developed to quantify the phenotypic changes that occur in an in vitro decidualization system.
[92]
[PDF] Quantitative analysis of cellular morphology during in vitro ...
Feb 15, 2023 · Decidualization is a differentiation process involving shape reorganization from a broblast to an epithelioid-like appearance of endometrial ...
[93]
Quantitative Analysis of Cellular Morphology During In Vitro ...
We found that 2 days afterstimulation are sufficient to detect statistical differences in morphology between decidualization induced and control cells. Here, we ...
[94]
Current strategies using 3D organoids to establish in vitro maternal ...
May 10, 2024 · This review summarizes the current strategies and advancements in the field of 3D organoids to establish maternal-embryonic interaction and implantation models
[95]
Novel models for investigation of maternal-fetal interactions
Dec 30, 2022 · Endometrial organoids and trophoblast organoids in 3D cultures offer new opportunities to study maternal-fetal interactions during crucial steps ...
[96]
Innate immune signaling in trophoblast and decidua organoids ...
Aug 17, 2022 · We developed matched trophoblast (TO) and decidua organoids (DO) from human placentas to define the relative contributions of these cells to antiviral defenses.Missing: ECM | Show results with:ECM
[97]
Innate immune signaling in trophoblast and decidua organoids ... - NIH
We found that whereas singly cultured DOs were permissive to HCMV infection, co-culturing of DOs and TOs led to a significant reduction in HCMV infection in DOs ...Missing: ECM | Show results with:ECM
[98]
Excessive progesterone impairs mouse decidualization via the Kyn ...
Our data show that decidualization in day 8 pregnant mice and artificial decidualization in day 8 pseudopregnant mice are impaired by 4 mg or 8 mg/mouse P4. The ...
[99]
Comparative Analysis of Mouse Decidualization Models at the ... - NIH
Aug 13, 2020 · Decidualization in mice is embryo-dependent, which is slightly different from decidualization in humans [2].
[100]
A study on regional differences in decidualization of the mouse ...
Decidualization then spreads rapidly throughout the AM region, forming the secondary decidual zone (SDZ). Meanwhile, stromal cells in the mesometrial (M) region ...
[101]
Pluripotent stem cell-derived endometrial stromal fibroblasts in a ...
May 18, 2021 · However, rodent models do not share fundamental biological processes with the human endometrium, such as spontaneous decidualization, and no ...
[102]
Metabolic reprogramming and heterogeneity during the ...
Jul 30, 2024 · Our results revealed that the differentiation of stromal cells into decidual cells is accompanied by increased amino acid and sphingolipid metabolism.
[103]
https://www.mdpi.com/2218-273X/15/6/873
[104]
Molecular Basis of Impaired Decidualization in the Eutopic ... - NIH
Feb 21, 2025 · Multiple studies have demonstrated impaired decidualization in the eutopic endometrium of women with endometriosis.
[105]
Regulation of the SUMO pathway sensitizes differentiating human ...
Our findings demonstrate how dynamic changes in the SUMO pathway mediated by cAMP signaling determine the endometrial response to progesterone.<|separator|>
[106]
SOX4 facilitates PGR protein stability and FOXO1 expression ... - eLife
Mar 4, 2022 · We document that SOX4 is a key regulator of human endometrial stromal cells decidualization by directly regulating FOXO1 expression.
[107]
Targeting Cellular Senescence to Enhance Human Endometrial ...
Senolytic treatment modulates decidualization in human endometrial stromal cells. Biochem. Biophys. Res. Commun. 2021, 571, 174–180. [Google Scholar] ...
[108]
Senolytic treatment modulates decidualization in human ... - PubMed
Sep 24, 2021 · Decidualization - the differentiation of endometrial stromal cells (ESCs) into decidual cells - is a crucial step for successful embryo ...
[109]
p53 coordinates decidual sestrin 2/AMPK/mTORC1 signaling ... - JCI
Jul 25, 2016 · Our study shows that an inverse relationship between AMPK activation and mTORC1 signaling is organized in the decidua by Met or Rsv treatment to ...
[110]
Energy metabolism and maternal-fetal tolerance working in ... - NIH
Jun 19, 2023 · These observations indicate that normal level of glucose is indispensable for decidualization. In addition, uterus can store glucose as glycogen ...
[111]
Metabolic reprogramming and heterogeneity during the ... - PubMed
Jul 30, 2024 · Here, we integrated the single-cell RNA sequencing (scRNA-seq) datasets on the endometrium of the menstrual cycle and the maternal-fetal ...
[112]
Artificial intelligence model utilizing endometrial analysis to ...
Apr 24, 2024 · Discussion. The results of this analysis suggest that the endometrial implantation potential may be predicted using AI.Missing: decidualization | Show results with:decidualization
[113]
Premature expression of the decidualization marker prolactin is ...
Our study demonstrates that premature expression of the decidualization marker PRL during the luteal phase is associated with RIF.Missing: trials | Show results with:trials
[114]
Uterine HOXA11 antisense long non-coding RNA prevents ...
Mar 22, 2022 · Progesterone, in turn, drives endometrial differentiation, also known as decidualization. The decidualized endometrium is briefly receptive to ...