The development of the cerebral cortex encompasses the intricate series of cellular and molecular events that transform a uniform sheet of neuroepithelial progenitors into the folded, layered structure essential for sensory processing, motor control, and higher cognition in mammals, particularly humans, beginning around 6 weeks post-conception and extending through gestation and into early postnatal life.[1] This process, conserved across species yet uniquely expanded in primates, involves sequential stages of neurogenesis, where neural stem cells generate neurons; migration, in which neurons travel to their destinations; differentiation, forming distinct laminar and areal identities; and maturation, including synaptogenesis and circuit refinement.[2] Key to this is the "inside-out" layering pattern, where deeper cortical layers (V–VI) form first, followed by superficial ones (II–IV), enabling the cortex's functional specialization.[3]Neurogenesis primarily occurs in the proliferative zones of the ventricular zone (VZ) and subventricular zone (SVZ), driven by radial glial cells (RGCs) that serve as neural progenitors, producing excitatory projection neurons and, in humans, a diverse array of intermediate progenitors in an expanded outer SVZ (oSVZ) that contributes to cortical enlargement and gyrification.[1] In humans, this peaks during the second trimester, generating over 80% of cortical neurons prenatally, with genetic factors like transcription factors (e.g., PAX6, EMX1/2) regulating progenitor proliferation and initial areal patterning via intrinsic protomap mechanisms.[2] Following generation, neurons migrate radially along glial scaffolds or tangentially from subcortical sources like the ganglionic eminence, reaching the cortical plate in 3–7 days in primates, guided by molecular cues such as Eph/ephrin signaling to establish laminar positions.[1]Arealization, the specification of functional regions (e.g., sensory vs. association areas), integrates intrinsic genetic programs—evident in spatiotemporal gene expression gradients—with extrinsic signals from thalamocortical axons that arrive at the transient subplate layer by mid-gestation, refining boundaries through activity-dependent mechanisms.[3] Human-specific adaptations, including the gene ARHGAP11B promoting basal progenitor amplification and enhanced oRGC diversity, underlie the cortex's disproportionate expansion, particularly in prefrontal and association areas, supporting advanced cognition.[2] Postnatally, the cortex undergoes extensive remodeling: synaptogenesis surges from gestational week 20, peaking at 3–15 months depending on the region, followed by selective pruning to optimize connectivity, with myelination continuing into adolescence.[1]Disruptions in these processes, such as Zika virus targeting RGCs or genetic mutations affecting migration, can lead to neurodevelopmental disorders like microcephaly or lissencephaly, highlighting the precision required for normal cortical architecture.[2] Recent advances in single-cell transcriptomics and cerebral organoids, including 2025 developments in whole-brain organoids with vascularization and studies on electrical activity emergence, have illuminated these dynamics, revealing clonal lineages and molecular diversity that shape connectivity and underscoring the evolutionary divergence in human cortical development.[1][4][5]
Embryonic Origins
Neural Induction and Tube Formation
The development of the cerebral cortex begins during early embryogenesis with neural induction, a process triggered by gastrulation where the notochord, derived from the primitive streak, induces the overlying ectoderm to form the neural plate. This induction occurs through the inhibition of bone morphogenetic protein (BMP) signaling by secreted factors such as noggin, chordin, and follistatin from the notochord and organizer regions, which dorsalize the ectoderm and promote its commitment to a neural fate. Concurrently, Wnt and fibroblast growth factor (FGF) signaling pathways contribute to this process by modulating cell proliferation and patterning in the ectoderm, ensuring a stable neural identity while suppressing epidermal differentiation.Neurulation follows, involving the folding and elevation of the neural plate to form the neural tube, the precursor structure to the entire central nervous system, including the future cerebral cortex. In mice, this occurs around embryonic days 8-9 (E8-E9), corresponding to weeks 3-4 of human gestation, when the neural folds converge and fuse dorsally, starting at the hindbrain level and progressing rostrally and caudally. The process is mediated by cytoskeletal changes, extracellular matrix remodeling, and apical constriction of neuroepithelial cells, culminating in the enclosure of the neural plate into a tube with a central lumen that will expand into the ventricular system.As the neural tube forms, the anterior-posterior axis is established, with the anterior region specified as forebrain through the expression of transcription factors such as Otx2 and Foxg1, which define the rostral identity and initiate regionalization for cerebral structures. Otx2 is expressed early in the anterior neural plate, promoting forebrain development, while Foxg1 later restricts posterior influences and supports telencephalic expansion. Disruptions in neural tube closure, such as failure of fusion leading to anencephaly, serve as early indicators of potential cortical malformations, often resulting from genetic or environmental factors like folate deficiency that impair these inductive and folding processes. This foundational tube formation sets the stage for subsequent telencephalic progenitor development.
Telencephalic Specification
The specification of the telencephalon begins with dorsal-ventral (DV) patterning of the forebrain, primarily driven by Sonic hedgehog (Shh) signaling from the prechordal plate and ventral midline structures. Shh, secreted by the prechordal mesoderm, induces ventral midline identities in the forebrain and establishes a ventral-to-dorsalgradient that promotes ventral fates while restricting dorsal ones, thereby delineating the boundary between the diencephalon and telencephalon.[6] This gradient is crucial for the formation of the pallium, the dorsal telencephalic primordium that gives rise to the cerebral cortex, as Shh suppresses pallial gene expression in ventral regions.[7] In Shh-null mice, ventral forebrain structures fail to develop, resulting in a dorsalized telencephalon with expanded pallial territories.[7]In the dorsal telencephalon, transcription factors such as Emx1, Emx2, and Pax6 are expressed to specify the neocortical field. Emx1 and Emx2 initiate expression around embryonic day 9.5 (E9.5) in mice, with high levels in the caudomedial pallium, while Pax6 appears slightly later around E10, predominantly in the rostrolateral regions. These factors establish graded expression patterns that define the neocortical primordium, with Emx2 promoting posterior cortical identities and Pax6 favoring anterior ones.[8] In humans, equivalent expression begins around gestational week 5, coinciding with early telencephalic expansion.[9] Genetic studies show that loss of Emx2 shifts areas toward anterior fates, while Pax6 mutations expand posterior domains, highlighting their opposing roles in neocortical field delineation.[8]Telencephalic specification also involves interactions at the diencephalon-telencephalon boundary, mediated by Fgf8 and Wnt signaling. Fgf8, expressed in the anterior neural ridge and commissural plate, provides anterior-posterior cues that pattern the telencephalon and restrict diencephalic fates, ensuring proper boundary formation.[10] Wnt signaling, active in dorsal midline structures like the cortical hem, reinforces telencephalic identity by promoting proliferation and inhibiting diencephalic expansion at the boundary.[11] In Wnt pathway mutants, telencephalic domains encroach on diencephalic territories, disrupting overall forebrain organization.[12]The initial allocation of cortical areas within the pallium relies on gradient-based positional information from these signaling molecules and transcription factors. Morphogen gradients, such as those of Fgf8 (high anteriorly) and Emx2 (high posteriorly), provide intrinsic cues to progenitors, establishing a protomap of areal identities before neuronal migration.[13] This graded system allows for progressive specification of proto-areas, with factors like Pax6 and Coup-tfi further refining anterior versus posterior domains.[13] Such positional encoding sets the foundation for later areal diversification, independent of early proliferation dynamics.[14]
Progenitor Proliferation
Ventricular Zone Dynamics
The ventricular zone (VZ) serves as the primary germinal zone for cortical neurogenesis, comprising a pseudostratified epithelium of apical progenitors primarily in the form of radial glia cells that span from the ventricular surface to the pial surface. These radial glia function as neural stem cells, undergoing interkinetic nuclear migration and mitotic divisions at the apical surface to generate the initial pool of cortical neurons. In mice, proliferative activity in the VZ initiates around embryonic day (E) 10, with radial glia predominantly executing symmetric divisions between E10 and E12 to expand the progenitor population before shifting toward neurogenic output.[15][16]Symmetric divisions produce two radial glia daughters, thereby amplifying the stem cell pool, while asymmetric divisions yield one radial glia that self-renews and one daughter cell destined for differentiation, either as a neuron or an intermediate progenitor. This balance is crucial for establishing the founder population of cortical progenitors. The orientation and mechanics of these divisions, often involving oblique or horizontal cleavages, ensure the inheritance of key cellular components like the basal process, maintaining progenitorpolarity and scaffold integrity.[15][17]Notch signaling is a pivotal regulator of VZ dynamics, promoting the maintenance of radial glia stemness by repressing neuronal differentiation genes and sustaining proliferative capacity through lateral inhibition among neighboring progenitors. Disruption of Notch pathway components, such as RBP-Jκ, leads to premature depletion of the VZ progenitor pool and accelerated neurogenesis. Complementing this, cell cycle regulators like cyclin D2 drive G1/S phase progression in radial glia, enabling sustained proliferation; its asymmetric inheritance during divisions further influences daughter cell fates.[18][19]By around E11 in mice, the VZ begins generating the first postmitotic neurons, which migrate to form the preplate, alongside intermediate progenitors that detach from the ventricular surface to contribute to later amplification of neuron production. This marks the transition from progenitor expansion to active neurogenesis. The peak proliferative phase of the VZ ultimately accounts for approximately 30% of cortical neurons directly in rodents, with indirect contributions via the SVZ amplifying total output from VZ-originated lineages.[20] In humans, equivalents occur during gestational weeks 6-12 when radial glia in the VZ initiate telencephalic patterning and early layering.[21]
Subventricular Zone Emergence
The subventricular zone (SVZ) emerges as a secondary germinal zone in the developing cerebral cortex, arising from the delamination of basal progenitors from the primary ventricular zone (VZ). In mice, this process begins around embryonic day 13 (E13), when intermediate neuronal progenitors lose apical contacts and migrate basally to populate the nascent SVZ, thereby amplifying the progenitor pool beyond the apical VZ divisions.[22] In humans, SVZ differentiation is evident by postconceptional week 8, marking the onset of a more complex proliferative architecture that supports the expanded cortical size characteristic of primate brains.[23]These basal progenitors, often identified as Tbr2-positive intermediate progenitors, undergo symmetric neurogenic divisions within the SVZ to generate neurons destined primarily for the upper cortical layers (II-IV). This Tbr2+ lineage enhances neuronal output, with the SVZ contributing approximately 70% of cortical neurons in rodents.[20] In contrast, lissencephalic species like rodents exhibit a relatively modest SVZ, limited to an inner SVZ (iSVZ) with fewer progenitor types.[24]Gyrencephalic brains, such as those of humans and ferrets, feature an additional outer SVZ (OSVZ) that further diversifies and expands this zone, harboring outer radial glia (oRGs) that resemble ventricular radial glia but lack apical processes. oRGs comprise a majority of basal progenitors in the primate OSVZ and drive protracted neurogenesis through self-renewing divisions and shorter cell cycles, contributing the majority of upper-layer neurons in humans and facilitating cortical folding.[25] Human-specific adaptations, such as ARHGAP11B expression, further amplify oRG production.[2] This OSVZ expansion correlates with increased cortical surface area and gyrification, distinguishing gyrencephalic from lissencephalic cortices where such a distinct outer layer is absent.[26]SVZ development and progenitor proliferation are regulated by extrinsic growth factors, notably insulin-like growth factor 2 (Igf2), which is secreted into the cerebrospinal fluid by the choroid plexus and promotes symmetric proliferative divisions in both VZ and SVZ progenitors. Igf2 signaling via IGF1R enhances cell cycle progression and survival, ensuring robust SVZ formation; its disruption leads to reduced progenitor pools and cortical hypoplasia. Other factors, such as fibroblast growth factors, further modulate SVZ dynamics, integrating environmental cues to fine-tune neuronal production across species.[27]
Cortical Stratification
Preplate and Subplate Formation
The development of the cerebral cortex begins with the formation of the preplate, a transient early layer that emerges from the initial cohort of postmitotic neurons generated in the ventricular zone. In mice, this occurs around embryonic day 11 to 12 (E11–E12), when these pioneer neurons migrate superficially and settle above the ventricular zone to form a loose, superficial stratum beneath the pia mater.[28] This preplate represents the primordial cortical structure, comprising Cajal-Retzius cells and other early-born neurons that provide a scaffold for subsequent cortical organization.[28]By embryonic day 13 (E13) in mice, the preplate undergoes splitting as a new wave of pioneer neurons, destined for future layer VI, migrates into its midst, cleaving it into three distinct compartments: the superficial marginal zone (which will become layer I), the emerging cortical plate, and the deeper subplate.[28] This process, driven by the radial migration and coalescence of these neurons rather than translocation, establishes the basic framework for cortical lamination and proceeds in a lateral-to-medial gradient across the neocortex.[29] The subplate, in particular, consists of early-generated, postmigratory neurons that transiently populate this layer, playing a pivotal role in guiding incoming afferents.[28]Subplate neurons function as temporary waypoints for thalamocortical axons, which arrive from the thalamus around E14 in mice and pause within the subplate before invading the cortical plate by E18, ensuring precise targeting to layer IV.[30] Experimental ablation of subplate neurons disrupts this guidance, causing axons to overshoot their targets and persist in the white matter, underscoring their essential role in circuit formation.[30] Following successful innervation of the cortical plate, most subplate neurons undergo programmed cell death (apoptosis) around birth, allowing the subplate to largely dissipate as a transient feature of development.[31]In humans, the preplate forms during the seventh gestational week, marking the onset of neocortical stratification with pioneer neurons assembling beneath the meninges.[28] Splitting of the preplate into the marginal zone, cortical plate, and subplate initiates between weeks 7 and 8, paralleling the murine timeline but on a protracted scale.[28] The subplate matures significantly by week 20, expanding to become a prominent compartment that supports thalamocortical wiring and is crucial for cortical arealization, the process establishing functional regions such as sensory and motor areas.[28] This extended subplate phase in humans, lasting until the perinatal period, facilitates the complex connectivity required for the expanded neocortex.[28]
Cortical Plate Assembly
The cortical plate emerges as the foundational structure of the future six-layered neocortex, serving as the primary destination for radially migrating neurons generated in the ventricular and subventricular zones. In mice, this assembly process occurs primarily between embryonic days 12 and 17 (E12-E17), during which postmitotic neurons integrate into the plate to establish its laminar organization.[32] The plate initially forms above the preplate following its splitting, creating a compact zone where incoming neurons settle according to their birthdates, progressively building the cortical architecture from ventricular origins toward the pial surface.[32]A defining feature of cortical plate assembly is the inside-out gradient of neuronal addition, where early-generated neurons populate the deeper layers first, followed by later-born neurons in more superficial positions. Specifically, neurons destined for layers V and VI are born and settle between E12 and E14, forming the initial deep scaffold of the plate, while those for layers II-IV arise and integrate from E16 through postnatal day 0 (P0), bypassing prior layers to occupy upper strata. This birthdate-dependent settling ensures the sequential layering essential for cortical function, with each cohort of neurons halting migration at predetermined depths to avoid disrupting established circuitry.Reelin, secreted by Cajal-Retzius cells in the marginal zone, plays a crucial role in regulating this process by signaling migrating neurons to detach from radial glia and arrest at their appropriate laminar positions within the plate.[33] Disruptions in reelin signaling lead to inverted lamination, underscoring its necessity for proper inside-out assembly.[33]In humans, the cortical plate develops a markedly thicker structure compared to rodents, attributable to an extended period of neurogenesis that spans up to approximately 20 weeks of gestation, enabling the production of a vastly greater number of neurons to support the expanded cortical surface area.[34] This prolongation, lasting around 110-130 days from gestational weeks 8 to 25-26, contrasts sharply with the brief 5-6 day window in mice, contributing to species-specific differences in plate density and overall neocortical complexity.[34]
Neuronal Migration Mechanisms
Radial Migration via Glia
Radial migration via glia serves as the predominant mechanism by which projection neurons traverse from the ventricular zone (VZ) and subventricular zone (SVZ) to the cortical plate during cerebral cortex development. Newly generated postmitotic neurons utilize the elongated processes of radial glial cells as scaffolds, enabling directed radial movement toward the pial surface. This process ensures the inside-out layering of the cortex, with earlier-born neurons occupying deeper layers and later-born ones settling superficially.[35][36]The migration occurs through two distinct modes: glia-guided locomotion and soma translocation. In glia-guided locomotion, neurons exhibit a bipolar morphology, extending a leading process that adheres to and climbs along the radial glial fiber, followed by the translocation of the nucleus via actomyosin and microtubule-based forces. Soma translocation, often employed by early-born neurons, involves the extension of a static leading process that anchors to the pial basement membrane, pulling the cell body forward independently of direct glial contact, at speeds up to 60 μm/h. These modes transition dynamically, with locomotion dominating as the cortex thickens.[37][36][35]Adhesion between neurons and radial glia is mediated by key proteins such as integrins (e.g., α3β1 for motility and αVβ1 for scaffold maintenance) and astrotactins, which facilitate dynamic attachment and detachment. Microtubule dynamics, essential for nuclear movement and process extension, are regulated by doublecortin (DCX) and lissencephaly-1 (LIS1), which stabilize cytoskeletal elements and dynein motor activity. In mice, this migration initiates around embryonic day 12 (E12) for the first projection neurons, peaking at E14–E16 during deep layer formation.[35][36]In humans, the protracted neurogenesis and expanded progenitor pools result in longer radial glial fibers, often spanning millimeters across the developing cortex, which support tangential expansion of neuronal columns and contribute to gyral folding. Approximately 80% of cortical projection neurons rely on this radial route to populate the six-layered neocortex. Disruptions, such as mutations in FLNA, DCX, or LIS1, impair scaffold integrity or cytoskeletal function, leading to periventricular heterotopia where ectopic neuronal clusters accumulate near the ventricles.[35][36][38]
Tangential Migration Pathways
Tangential migration refers to the orthogonal movement of GABAergicinterneurons from subcortical origins into the developing cerebral cortex, distinct from the radial migration of excitatory pyramidal neurons along glial scaffolds. These interneurons, comprising approximately 20-30% of all cortical neurons, primarily originate from progenitor cells in the medial ganglionic eminence (MGE), lateral ganglionic eminence (LGE), and caudal ganglionic eminence (CGE) within the ventral telencephalon.[39] The MGE serves as the major source, contributing 50-60% of cortical interneurons in rodents, including parvalbumin-positive (PV+) and somatostatin-positive (SOM+) subtypes, while the CGE provides 30-40% of non-fast-spiking interneurons such as those expressing vasoactive intestinal peptide (VIP).[40] This migratory process populates the cortex with inhibitory neurons essential for circuit balance, with early demonstrations of tangential pathways revealing that young neurons traverse the cortical intermediate zone perpendicular to radial glia, often making transient contacts with glial fibers or axons.[41]Interneurons employ two primary modes of tangential migration: chain migration and somal translocation, both independent of radial glial guidance. In chain migration, cohorts of interneurons form dynamic, interconnected streams—such as the superficial route through the marginal zone (MZ) and the deep route via the subventricular zone (SVZ)—advancing collectively through homotypic contacts mediated by adhesion molecules like neuroligin-neurexin interactions.[42] Somal translocation involves the forward extension of a leading process, followed by nucleokinesis where the centrosome and Golgi apparatus precede the nucleus, enabling rapid soma advancement without extensive process elongation; this mode predominates in confined streams and allows navigation around obstacles.[42] These mechanisms facilitate long-distance travel, with interneurons dispersing tangentially across cortical domains while avoiding ectopic invasion of adjacent structures like the striatum.[43]Migration is precisely guided by chemoattractive and repulsive cues that establish migratory corridors and direct trajectories. The chemokine CXCL12, secreted by meningeal cells, choroid plexus, and SVZ astrocytes, binds CXCR4 receptors on interneuron somata to attract and confine cells to streams, while CXCR7 modulates signaling to prevent premature cortical plate entry. Repulsive signals, including Slit1 and Slit2 ligands from the septum and ventral telencephalon, activate Robo1 receptors on interneurons to deter deviation toward the ventricular zone or striatum, often in concert with semaphorin-3A via neuropilin-1 co-receptors.80801-6) In mice, tangential migration initiates around embryonic day (E) 13.5, with streams forming between E12 and E16, and initial arrivals in the cortex by E16; in humans, tangential migration begins around gestational weeks 8-9 and continues through the second and third trimesters, with peak influx during weeks 12-20.[42][20]Upon reaching the cortex, MGE-derived interneurons, predominantly fast-spiking PV+ cells, integrate into layers II-VI, exhibiting a bias toward deeper layers (IV-VI) but distributing across all strata to form basket and chandelier morphologies with non-adapting action potentials.[44] CGE- and LGE-derived interneurons contribute to superficial layers (II-III), ensuring layer-specific inhibition.[40] This integration completes the tangential phase, setting the stage for laminar positioning without further tangential dispersion.[43]
Layer-Specific Differentiation
Layer I and Marginal Zone
The marginal zone, initially formed as the outermost layer of the early cortical preplate, evolves into Layer I during corticogenesis, primarily through the contributions of early-generated Cajal-Retzius (CR) cells that populate this region and secrete reelin starting around embryonic day 10.5 (E10.5) in mice. These CR cells, among the first neurons born in the developing cortex, emerge mainly between E10.5 and E11.5 from progenitor zones such as the cortical hem and pallium-subpallium boundary, with their population expanding through E13 to establish the reelin-rich marginal zone that guides subsequent layering.[45][46][47]Layer I is characterized by a sparse neuronal population, comprising less than 0.5% of cells in a cortical column, dominated by GABAergicinterneurons including CR cells and other inhibitory subtypes, embedded within a rich extracellular matrix. This composition supports the regulation of columnar organization by providing a scaffold for radial migration and modulating local inhibition during circuit assembly.[48][49]CR cells play a transient role in cortical arealization, influencing the tangential distribution of neurons and connectivity patterns. Following their peak function in late embryogenesis, most CR cells undergo programmed degeneration in the postnatal cortex, primarily through apoptosis, ensuring the maturation of Layer I into a predominantly input-receptive zone devoid of these pioneer neurons.In humans and other primates, Layer I is notably thicker relative to rodents, accommodating an expanded diversity of neurons and supporting broader apical dendrite fields of pyramidal cells from deeper layers, which enhances integrative capacity in the enlarged primatecortex.[50]
Deep Layers V and VI
The deep layers V and VI of the cerebral cortex are the first to form during corticogenesis, comprising early-born projection neurons essential for subcortical connectivity. In mice, neurons destined for these layers are generated between embryonic days E12.5 and E14.5 from progenitors in the ventricular zone. Layer VI primarily consists of corticothalamic projection neurons that send axons to the thalamus, while layer V includes subcerebral projection neurons such as corticospinal motor neurons targeting the spinal cord and corticopontine neurons innervating the pons. These neurons migrate radially to their positions in an inside-out sequence, forming the foundational scaffold for cortical architecture.Specification of neuronal identity in these layers is governed by key transcription factors. Tbr1 is prominently expressed in layer VI corticothalamic neurons, promoting their differentiation while repressing subcerebral fates by downregulating Fezf2 expression. Conversely, high levels of Fezf2 drive the identity of layer V subcerebral projection neurons, enabling their distinct subcortical targeting. Upon reaching their laminar destinations, these neurons settle through a reelin-mediated pause in migration; reelin, secreted by Cajal-Retzius cells in the marginal zone, acts as a stop signal by inducing phosphorylation of n-cofilin in neuronal leading processes, thereby stabilizing the actincytoskeleton and halting radial translocation.Following settlement, axonal outgrowth from deep-layer neurons commences rapidly, establishing long-range projections critical for sensorimotor circuits. By E15 in mice, layer V corticospinal axons begin extending toward the spinal cord via the internal capsule, while layer VI corticothalamic axons target thalamic nuclei, laying the groundwork for thalamocortical feedback loops. These early projections are vital for coordinating motor output and sensory relay, with disruptions leading to impaired circuit function.In humans, the timeline for deep-layer formation is protracted compared to rodents, reflecting extended cortical expansion. Layer VI neurons, marked by TBR1 expression, emerge in the cortical plate by 8-9 postconceptional weeks (PCW), with layer V differentiation initiating around 10 PCW as the subplate becomes distinct. Maturation of these layers continues through the second trimester, supporting prolonged synaptogenesis and circuit refinement unique to primate brain development.
Superficial Layers II-IV
The superficial layers II-IV of the cerebral cortex, generated later in development compared to deeper layers, primarily comprise projection neurons involved in intracortical and thalamocortical connectivity. In mice, neurons destined for these layers are born between embryonic day 14 (E14) and postnatal day 0 (P0), following an inside-out pattern where later-born cells occupy more superficial positions.[51] Layer IV, the granular layer that serves as the primary recipient of thalamocortical afferents, consists of neurons generated around E15.5 to E17.5.[52] Layers II and III, which include callosal and intratelencephalic projection neurons responsible for interhemispheric and associative connections, arise slightly later, from approximately E16 to P0.[51]Key transcription factors guide the specification and differentiation of these upper-layer neurons. Cux1 and Cux2, homeodomain proteins expressed selectively in postmitotic neurons of layers II-IV, regulate dendritic branching, spine morphology, and the specificity of corticocortical projections, ensuring proper laminar identity and circuit formation.[53] In layer IV, the orphan nuclear receptor Rorb (also known as RORβ) establishes transcriptional identity by promoting the expression of layer-specific genes and repressing deep-layer markers; its absence disrupts neuronal aggregation and thalamocortical afferent segregation.[54]A hallmark of layer IV development in the somatosensory cortex is the postnatal formation of barrels, discrete cytoarchitectonic units that align with whisker representations. These structures emerge around postnatal day 4 (P4) as patches of reduced cell density in the cortical plate, with septa becoming prominent by P6, driven by the segregation of thalamocortical inputs during the first postnatal week.[55] Layers II-IV collectively support dendritic lamination, where apical dendrites of pyramidal neurons align in laminar-specific patterns, facilitating local intracortical circuits for sensory integration and associative processing.[52]In humans, the superficial layers II-IV show marked evolutionary expansion, comprising approximately 50% of cortical thickness—compared to 19% in rodents—which enhances cortico-cortical connectivity and supports advanced cognitive functions such as language and abstract reasoning.[56] This expansion correlates with larger pyramidal neuron somata, more elaborate dendritic arbors, and higher spine densities in layers II-III, enabling recurrent computations critical for higher-order cognition.[56] Developmentally, upper-layer formation in humans occurs primarily during mid-gestation, with peak neurogenesis and radial migration of these neurons taking place between gestational weeks 12 and 20, after which lamination and circuit refinement continue.[57]
Regulatory Signaling
Reelin-DAB1 Cascade
The Reelin-DAB1 signaling cascade is a critical contact-dependent pathway that regulates neuronal migration and positioning during cerebral cortex development. Reelin, a large extracellular glycoprotein, is primarily secreted by Cajal-Retzius cells located in the marginal zone of the developing cortex. These cells release Reelin into the extracellular matrix, where it forms a gradient that guides migrating neurons toward the pial surface.[58][59][60]Reelin exerts its effects by binding to two low-density lipoprotein receptors on the surface of migrating neurons: apolipoprotein E receptor 2 (ApoER2) and very low-density lipoprotein receptor (VLDLR). This binding induces receptor clustering and recruits the intracellular adaptor protein Disabled-1 (DAB1) to the cytoplasmic tails of the receptors. Upon receptor activation, DAB1 undergoes tyrosine phosphorylation by Src family kinases, serving as a key scaffold for downstream effectors.[61][62][63]The phosphorylated DAB1 then activates several signaling branches essential for migration termination. One major pathway involves the phosphatidylinositol 3-kinase (PI3K)/Akt axis: phosphorylated DAB1 binds the p85 regulatory subunit of PI3K, leading to its activation and subsequent phosphorylation of Akt. Activated Akt inhibits glycogen synthase kinase 3β (GSK3β) by phosphorylating it at serine 9, which disrupts the neuronal cytoskeleton's attachment to radial glia scaffolds. This inhibition promotes the detachment of neurons from radial glia, allowing them to somatically translocate and halt migration at their appropriate laminar positions. Additionally, this cascade modulates N-methyl-D-aspartate receptor (NMDAR) trafficking and cytoskeletal dynamics via disabled homolog 2-interacting protein (DAB2IP), further stabilizing neuronal positioning.[64][65][66]The Reelin-DAB1 cascade is indispensable for the inside-out layering of the cerebral cortex, where later-born neurons migrate past earlier ones to form superficial layers. Disruptions in this pathway, as seen in reeler mice with RELN mutations, result in the classic reeler phenotype: inverted cortical lamination, with deep layers forming superficially and vice versa, accompanied by cerebellar ataxia and impaired dendrite maturation. Beyond migration, Reelin signaling promotes dendritic outgrowth and spine formation in post-migratory neurons by enhancing NMDA receptor function and actin polymerization through the PI3K pathway. In mice, Reelin expression and signaling activity peak between embryonic days 13 and 16, coinciding with the height of radial migration and layer assembly.[64][67][63]In humans, variants in the RELN gene have been associated with increased risk for schizophrenia, potentially through reduced Reelin expression and disrupted cortical layering or synaptic plasticity. These genetic links underscore the pathway's conserved role in neurodevelopment and its implications for psychiatric disorders.[68][69][70]
Morphogen Gradients (SHH, BMPs)
Morphogen gradients play a crucial role in patterning the developing cerebral cortex by establishing distinct progenitor domains along the dorsoventral axis. Sonic hedgehog (SHH), secreted from ventral sources such as the medial ganglionic eminence, forms a gradient that diffuses dorsally to regulate cell fate specification. In regions of low SHH concentration, such as the prospective neocortex, the absence of strong SHH signaling allows the accumulation of the Gli3 repressor form (Gli3R), which suppresses ventral gene expression and promotes dorsal identities, including neocortical progenitors. This gradient-dependent mechanism ensures the proper segregation of pallial (dorsal) and subpallial (ventral) domains during early telencephalic development.[71]Bone morphogenetic proteins (BMPs), particularly BMP7, originate from meningeal tissues and the cortical hem, creating a complementary gradient that influences cortical histogenesis. BMP signaling via Smad1/5/8 pathways promotes apoptosis in excess progenitors, thereby refining cell numbers, and biases surviving progenitors toward upper-layer neuronal fates by upregulating genes like Satb2 in the subventricular zone. These actions help transition the cortical ventricular zone (VZ) from deep-layer to superficial-layer neurogenesis. Disruptions in BMP signaling, such as in Noggin mutants, lead to overproliferation and altered layer formation, underscoring its role in maintaining progenitor balance.[72][73]The interplay of SHH and BMP gradients with other morphogens, such as fibroblast growth factor 8 (FGF8) from the anterior telencephalon, drives arealization by specifying functional cortical regions. Low dorsal SHH levels interact antagonistically with FGF8 to delineate rostrocaudal patterns, while BMPs from the caudomedial cortical hem counterbalance FGF8 to establish mediolateral identities. In mice, SHH exerts its primary patterning effects from embryonic day 9 to 12 (E9-E12), setting up progenitor domains, whereas BMP activity peaks later around E14 onward, coinciding with neurogenesis onset. In humans, analogous disruptions—such as SHH pathway mutations causing holoprosencephaly—perturb VZ/SVZ progenitor equilibrium, resulting in reduced cortical folding and layer imbalances that contribute to neurodevelopmental disorders.[74][75]
Developmental Disorders
Migration Defects (Lissencephaly)
Migration defects during cerebral cortex development primarily disrupt the radial translocation of neurons from the ventricular zone (VZ) to their laminar positions, leading to malformations such as lissencephaly, characterized by a smooth cerebral surface due to absent or reduced gyri and sulci.[76] Classical lissencephaly, also known as Type I lissencephaly, arises from mutations in the PAFAH1B1 gene (encoding LIS1), which encodes a regulatory subunit of platelet-activating factor acetylhydrolase and is essential for microtubule-based transport via cytoplasmic dynein during radial migration.[77] LIS1 mutations impair the coupling of dynein to the cytoskeleton, resulting in arrested neuronal migration and a thickened, four-layered cortex instead of the normal six-layered structure.[78]Miller-Dieker syndrome represents a severe form of classical lissencephaly caused by deletions encompassing LIS1 and contiguous genes on chromosome 17p13.3, leading to more pronounced agyria and facial dysmorphisms.[79] In contrast, cobblestone lissencephaly (Type II) stems from overmigration defects, where neurons breach the pial basement membrane due to glycosylation defects in extracellular matrix proteins; for instance, mutations in POMT1 disrupt α-dystroglycan glycosylation, causing neurons to invade the marginal zone and form a pebbled cortical surface.[80]Affected individuals typically present with intractable epilepsy starting in infancy, profound intellectual disability, developmental delays, and motor impairments such as hypotonia or spasticity.[81] Diagnosis is confirmed through magnetic resonance imaging (MRI), which reveals agyria (complete absence of gyri) or pachygyria (broad, flat gyri) with a simplified gyral pattern and thickened cortex.[82] The prevalence of lissencephaly is approximately 1 in 100,000 live births.[83]Mouse models, particularly Lis1 heterozygotes, recapitulate human pathology by exhibiting disrupted neuronal positioning in the VZ and subventricular zone (SVZ), reduced cortical layering, and migration delays without complete lethality.[76] These models highlight LIS1's dosage-sensitive role in centrosome integrity and mitotic spindle orientation during early corticogenesis.[84]
Proliferation and Folding Abnormalities
Abnormalities in the proliferation of neural progenitors and the subsequent folding of the cerebral cortex can lead to a range of malformations characterized by altered cortical size, architecture, and gyral patterns. These disruptions often stem from dysregulated signaling pathways that control cell division in the ventricular zone (VZ) and subventricular zone (SVZ), resulting in either excessive or insufficient neuronal production, which in turn affects the tangential expansion and radial organization necessary for proper gyrification. Such defects are implicated in neurodevelopmental disorders, where hyperproliferation may cause localized overgrowths or tubers, while aberrant folding can produce irregular sulci and gyri.[85]Primary microcephaly, an example of insufficient neuronal production, results from reduced proliferation of neural progenitors in the VZ, often due to mutations in genes such as MCPH1 (encoding microcephalin) or ASPM, which regulate mitotic spindle function and progenitor division. This leads to a smaller cerebral cortex with simplified gyral patterns and reduced neuronal numbers, affecting approximately 1 in 30,000–250,000 live births depending on the subtype, and is associated with intellectual disability and seizures.[86][87]Tuberous sclerosis complex (TSC) exemplifies proliferation abnormalities driven by genetic mutations in the TSC1 or TSC2 genes, which encode hamartin and tuberin, respectively, forming a complex that inhibits the mechanistic target of rapamycin (mTOR) pathway. Loss-of-function mutations in these genes lead to mTOR hyperactivation, promoting excessive proliferation of neural progenitors in the VZ and SVZ, and resulting in the formation of cortical tubers—hamartomatous lesions with disorganized lamination and giant cells. These tubers disrupt normal cortical layering and are a hallmark of TSC, contributing to epilepsy and cognitive impairments, with the condition affecting approximately 1 in 6,000 live births.[88][89][90]Polymicrogyria (PMG) and schizencephaly represent malformations involving excessive cortical folding, often linked to overproliferation or incomplete neuronal migration that secondarily affects gyrification. PMG is characterized by an overabundance of small, fused gyri due to abnormal overfolding of the cortical surface, leading to a simplified four-layered cortex instead of the typical six-layered structure. Mutations in the ARX gene, which encodes a homeoboxtranscription factor essential for interneuron development and cortical patterning, have been identified as a cause of PMG and related overfolding phenotypes in human malformations. Schizencephaly, featuring clefts lined by PMG, similarly arises from disruptions in progenitor dynamics that exaggerate folding patterns.[91][92][93]Megalencephaly, or macrocephaly due to cortical overgrowth, results from hyperactivation of the phosphatidylinositol 3-kinase (PI3K)/AKT pathway, which enhances progenitor proliferation in the SVZ, particularly the outer SVZ (OSVZ), leading to an enlarged cerebral cortex with increased neuronal numbers. Activating mutations in genes like PIK3CA drive this pathway's dysregulation, causing excessive expansion of basal progenitors and disrupted gyral formation, often manifesting as hemimegalencephaly or focal dysplasias. This overproliferation contrasts with microcephaly and underscores the pathway's role in scaling cortical surface area.[94][85]Studies suggest potential links between autism spectrum disorder (ASD) risk factors and altered proliferation of outer radial glia progenitors in the OSVZ, potentially contributing to cortical overgrowth and altered folding observed in a subset of ASD cases with macrocephaly. These findings, derived from human cortical organoid models and genomic analyses, suggest that OSVZ-specific disruptions may underlie connectivity anomalies in ASD, emphasizing the region's role in human-specific cortical expansion.[95][96]
Post-Embryonic Maturation
Synaptogenesis and Circuit Refinement
Synaptogenesis in the cerebral cortex begins prenatally but intensifies postnatally, establishing the foundational connectivity upon which embryonic layering provides the structural scaffold. In rodents, synapse formation peaks between postnatal days 7 and 14 (P7-P14), driven by rapid proliferation of excitatory and inhibitory synapses along developing dendrites. This process corresponds to the first year of life in humans, where synaptic density in the visual cortex reaches a maximum within a few months after birth, increasing at approximately 4% per week until 24-26 weeks post-menstrual age, followed by a sixfold surge to peak levels.[97][98]Activity-dependent mechanisms orchestrate this postnatal synaptogenesis, with NMDA receptors facilitating calcium influx to trigger synaptic strengthening and postsynaptic density protein 95 (PSD-95) stabilizing nascent synapses by anchoring AMPA receptors. PSD-95 knockdown in cortical neurons reduces AMPA-mediated excitatory postsynaptic currents to 58% and NMDA currents to 71% of control levels, while arresting spine density increases and elevating spine turnover rates.[99] These mechanisms ensure selective synapse maturation, particularly in layer IV of sensory cortices.Axonal arborization and dendritic spine maturation further refine cortical circuits postnatally, with thalamocortical axons elaborating branches to target layer IV pyramidal cells while intracortical axons form horizontal connections. In mice, thalamocortical synapses, marked by vesicular glutamate transporter 2 (VGlut2), compete with intracortical inputs (VGlut1) at dendritic spines, resolving multiple excitatory contacts (SMECs) that comprise up to 25% of spines at P14 into single-innervated mature spines by P25. Astrocytes contribute by secreting hevin, which promotes thalamo-cortical synapse formation and reduces SMECs, thereby stabilizing circuit specificity.[100] Dendritic spines undergo maturation from filopodial precursors to mushroom-shaped structures, increasing in size and stability through PSD-95 recruitment during this period.[99]Synaptic pruning refines these circuits by eliminating excess connections, primarily via microglia during critical periods of heightened plasticity. In the visual cortex, microglia preferentially engulf smaller, less active spines between P21 and P30, with contacts occurring at a rate of about one structure per hour, modulated by fractalkine signaling and complement proteins like C1q and C3. This activity-dependent pruning is essential for sensory map refinement, as blocking neuronal activity with tetrodotoxin reduces microglial interactions. Critical periods, such as P21-P30 in rodent visual cortex, close as perineuronal nets form around parvalbumin interneurons, constraining further plasticity.[101]In humans, synaptogenesis and refinement extend far beyond infancy, with prefrontal cortex (PFC) maturation prolonged into adolescence and early adulthood. Synaptic density in the PFC peaks around 3.5 years but undergoes extensive pruning starting in childhood, continuing dramatically through adolescence and slowing into the third decade, particularly in layer III. This extended timeline refines executive functions, with the PFC as the last region to mature, optimizing connectivity for complex cognition. Disruptions in these processes contribute to neurodevelopmental disorders; in autism spectrum disorder (ASD), excessive spine density and immature synapses persist due to impaired pruning, as seen in postmortem studies and iPSC-derived neurons from patients with mutations like FOXG1. Conversely, schizophrenia involves excessive pruning, reducing spine density in the PFC and leading to diminished glutamatergic connectivity, evident in iPSC models showing reduced PSD-95 expression.[102][103]
Recapitulation in Evolution and Disease Models
The development of the cerebral cortex exhibits recapitulation of evolutionary patterns, particularly in the expansion of progenitor zones that mirror phylogenetic adaptations across primates. In humans, the prolonged phases of the subventricular zone (SVZ) and outer subventricular zone (OSVZ) enable extensive neuronal production, driven by outer radial glia (oRG) cells, which amplify progenitor pools and contribute to cortical enlargement—a feature evolved in primates to support higher cognitive functions.[104] This ontogenetic process recapitulates phylogeny, as oRG cells and the OSVZ emerged in gyrencephalic mammals, with their density increasing from rodents to humans, correlating with gyral complexity and brain size. Seminal studies highlight how these zones' conical expansion in the human fetal cortex sustains basal progenitorproliferation, echoing evolutionary pressures for neocortical expansion observed in comparative analyses of primate brains.[105]Animal models, such as the ferret, effectively recapitulate cortical folding (gyrification), providing insights into mechanisms conserved from evolutionary intermediates. Ferrets, as gyrencephalic carnivores, undergo postnatal cortical folding similar to primates, with the outer SVZ expanding via intermediate progenitor cells under fibroblast growth factor (FGF) signaling, which regulates tangential expansion and sulcal formation.[106] This model has revealed that localized astrogenesis in the OSVZ supports vertical tension for gyri emergence, paralleling human developmental patterns.[107] In parallel, induced pluripotent stem cell (iPSC)-derived cerebral organoids model human-specific malformations by recapitulating ventricular and outer SVZ structures, including oRG-like progenitors, to study disruptions in lissencephaly-linked pathways.[108] These organoids exhibit heterotopia-like phenotypes in mutations such as EML1, offering a platform for human-exclusive traits absent in rodent models.[109]Disease models further illustrate evolutionary recapitulation, as disruptions in conserved pathways yield phenotypes that inform both ontogeny and phylogeny. The Reeler mouse, lacking Reelin expression, displays inverted cortical layering and subcortical band heterotopia, modeling human lissencephaly 2 (LIS2).[110] Similarly, iPSC-derived organoids from tuberous sclerosis complex (TSC) patients recapitulate cortical tubers through mTORC1 hyperactivation in progenitors, leading to enlarged, dysmorphic neurons and glia that mimic focal malformations.[111] Evolutionary perspectives link such vulnerabilities to genes like FOXP2, whose variants influence cortical gray matter density and language-related networks; disruptions may heighten schizophrenia risk by altering progenitor dynamics inherited from human-specific adaptations.[112][113]