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Integron

An integron is a bacterial genetic element that functions as a versatile platform for the capture, expression, and rearrangement of mobile gene cassettes—compact DNA segments typically encoding a single functional gene—via site-specific recombination.^[1] This mechanism, driven by a site-specific tyrosine recombinase called integrase, enables bacteria to acquire exogenous genetic material and adapt rapidly to environmental challenges, including antibiotic exposure.^[2] The basic architecture of an integron includes a conserved 5' region with the intI gene encoding the integrase, the attI recombination site where cassettes integrate, and the Pc promoter that transcribes captured genes.^[3] Gene cassettes themselves feature an attC recombination site, which forms a stable hairpin structure in single-stranded DNA to facilitate integration or excision, often positioning the gene under the control of the integron promoter.^[1] Many integrons also contain a 3' conserved segment with genes conferring additional resistances, such as to disinfectants or sulfonamides.^[3] Integrons are broadly categorized into mobile integrons, which are associated with plasmids or transposons and drive the horizontal transfer of adaptive traits, and chromosomal (or super-) integrons, which are embedded in bacterial genomes and can store vast arrays of up to hundreds of cassettes for long-term evolutionary potential.^[1] Mobile integrons are further divided into classes based on intI sequence homology, with class 1 being the most prevalent and clinically significant, followed by classes 2 and 3; classes 4 and 5 are rarer and linked to specific mobile elements.^[4] These elements have been identified in over 15% of sequenced bacterial genomes, predominantly in Proteobacteria, and play a pivotal role in generating genomic diversity through cassette shuffling and exchange.^[1] Particularly notable is the contribution of integrons to antimicrobial resistance, where mobile class 1 integrons vector nearly all known resistance gene cassettes in Gram-negative pathogens, facilitating the assembly of multidrug-resistant profiles.^[5] They have been detected in 22% to 59% of clinical isolates from Gram-negative bacteria, amplifying the global challenge of antibiotic resistance through efficient gene dissemination across bacterial populations and environments.^[2]

Overview

Definition

An integron is a bacterial genetic element characterized as a site-specific recombination system that enables the capture, excision, and expression of mobile gene cassettes through the action of an integrase enzyme.^[6] These elements are prevalent in diverse bacterial species and facilitate the assembly of functional gene arrays by integrating exogenous DNA modules.^[6] The core structure of an integron includes three essential components: the intI gene, which encodes the site-specific tyrosine recombinase (integrase) responsible for recombination events; the attI site, serving as the primary integration point for incoming cassettes; and the Pc promoter, positioned upstream of the attI site to drive the transcription and expression of integrated cassettes.^[6] Unlike transposons, which possess transposase enzymes for autonomous mobility, integrons lack self-encoded mobility functions and instead rely on association with other mobile genetic elements, such as plasmids or conjugative transposons, for dissemination.^[6] Gene cassettes within integrons consist of promoterless open reading frames (ORFs) that encode adaptive functions, such as antibiotic resistance or virulence factors, and are flanked by attC recombination sites to allow precise integration and excision.^[6] This modular architecture underscores integrons' role in bacterial adaptation to environmental pressures, though their broader evolutionary impacts are explored elsewhere.^[6]

Biological Significance

Integrons significantly enhance bacterial fitness by enabling the rapid acquisition and expression of adaptive gene cassettes, allowing bacteria to respond to environmental pressures such as antibiotic exposure and thereby improving survival rates in hostile conditions.^[7] This mechanism promotes evolutionary adaptability, as integrons function as dynamic platforms for horizontal gene transfer, capturing diverse genes that confer selective advantages in fluctuating ecosystems.^[1] For instance, in pathogenic bacteria, this gene mobilization facilitates quick adjustments to stressors, underscoring integrons' role in driving bacterial evolution and persistence.^[8] A primary ecological impact of integrons lies in their promotion of multidrug resistance, with class 1 integrons frequently detected in over 20% of clinical bacterial isolates, particularly among Gram-negative pathogens like Escherichia coli and Pseudomonas aeruginosa.^[9] This prevalence exacerbates the global antimicrobial resistance crisis, as integrons assemble and disseminate resistance determinants, enabling bacteria to evade multiple therapeutic agents simultaneously.^[10] Metagenomic analyses from 2023 have profiled over 130 antibiotic resistance genes associated with integrons, highlighting their quantitative contribution to resistance diversity across bacterial populations.^[11] Beyond resistance, integrons facilitate broader adaptations by incorporating cassettes that encode virulence factors, enhancing pathogen infectivity and host colonization.^[12] They also support metabolic versatility, capturing genes for novel enzymatic pathways that aid bacteria in utilizing alternative nutrients or tolerating pollutants in diverse niches like sediments or wastewater.^[12] Recent 2025 discoveries further reveal integrons as reservoirs for anti-phage defense systems, with chromosomal and mobile integrons stocking streamlined cassettes that protect against viral predation, thus bolstering bacterial community resilience in phage-rich environments.^[13]

History

Discovery

Integrons were first identified in the late 1980s on conjugative plasmids in Gram-negative bacteria, where they were linked to clusters of antibiotic resistance genes, facilitating their dissemination among pathogens. These elements were recognized for their ability to capture and express multiple resistance determinants, contributing to multidrug resistance in clinical isolates.^[14]^[3] In 1989, H.W. Stokes and R.M. Hall coined the term "integron" to describe a novel family of potentially mobile DNA elements encoding site-specific recombination functions, based on their analysis of structures in Serratia marcescens. Their work identified the integrase gene intI and the recombination sites attI and attC, establishing integrons as platforms for gene cassette integration downstream of a promoter for expression. This discovery highlighted integrons' role in assembling resistance gene arrays via tyrosine recombinase-mediated recombination.^[15]^[16] During the 1990s, integrons were increasingly recognized as chromosomal elements beyond plasmids, expanding their perceived evolutionary role. The first descriptions of such chromosomal integrons appeared in Vibrio cholerae, where large arrays of gene cassettes were noted in the bacterial chromosome, distinct from the smaller mobile forms associated with resistance.^[17]^[18] A key milestone came in 1998, when studies confirmed the mobility of integron gene cassettes through site-specific recombination, demonstrating that the purified IntI1 integrase binds to attI and attC sites to catalyze cassette excision and insertion with high specificity. This mechanism underscored integrons' function as dynamic gene capture systems, primarily for antibiotic resistance in early characterizations.

Key Developments

In 2009, researchers discovered that the bacterial SOS response, a global regulatory network activated by DNA damage, directly controls the expression of integron integrases through the repressor protein LexA binding to specific sites in their promoters, thereby linking environmental stress to the mobilization of gene cassettes and enhancing adaptive potential under genotoxic conditions.^[19] During the 2010s, studies expanded the understanding of integrons beyond mobile elements initially associated with plasmids, identifying large arrays resembling superintegrons embedded in bacterial chromosomes across diverse taxa, which serve as reservoirs for functional gene cassettes; however, the term "superintegron" has been discouraged in favor of "chromosomal integrons" to avoid implying exaggerated mobility.^[1] A 2023 metagenomic analysis profiled integron-associated antibiotic resistance, cataloging 136 distinct gene cassettes conferring resistance to eight major antibiotic families and disinfectants, distributed across multiple bacterial phyla and highlighting the vast functional diversity captured by these elements in natural microbial communities.^[11] In 2025, a pivotal study demonstrated that integron cassettes in both mobile and chromosomal integrons encode compact anti-phage defense systems, such as retrons and toxin-antitoxin modules, expanding the known roles of integrons from primarily antibiotic resistance to broader microbial immunity against viral threats and underscoring their versatility as evolutionary toolkits.^[13]

Molecular Structure

Core Components

The core platform of an integron consists of three essential elements: the intI gene, the attI recombination site, and the Pc promoter.^[20] These components form the foundational architecture that enables the capture and expression of mobile gene cassettes.^[21] The intI gene encodes a tyrosine recombinase known as integrase (IntI), which catalyzes site-specific recombination events between recombination sites.^[20] This enzyme is highly conserved across integron classes, with class 1 integrons exhibiting over 99% nucleotide identity in intI1 sequences, though certain variants can influence recombination efficiency.^[21] The attI site serves as the primary integration hotspot for gene cassettes and is characterized by a 7-bp core sequence (GTTRRRY) flanked by imperfect inverted repeats that facilitate integrase binding.^[21] In class 1 integrons, attI spans approximately 65 bp, while class 3 variants are longer at around 131 bp, but the core structure remains conserved to support recombination.^[21] The Pc promoter is a strong, outward-facing promoter embedded within the attI region or intI gene, responsible for driving the transcription of integrated gene cassettes.^[20] Variants such as PcW (weak), Pc2, and Pc3 exhibit differing strengths, with weaker forms like Pc2 and Pc3 associated with reduced cassette expression but potentially higher recombination activity in class 1 integrons.^[22] In class 2 integrons, Pc2 includes sub-variants like Pc2A and Pc2B, while class 3 features Pc3.^[21] Optional elements include the Pint promoter, which regulates intI expression and often overlaps with Pc, leading to transcriptional interference, and is responsive to the bacterial SOS response via LexA binding sites.^[20] A recombination directionality factor, analogous to excisionases in other systems, is rare in integrons and typically absent, with directionality instead governed by the structural asymmetry of recombination sites.^[21]

Gene Cassettes

Gene cassettes represent the modular, mobile components captured and rearranged by integrons, serving as repositories for diverse genetic functions in bacteria. Each cassette typically comprises a single open reading frame (ORF) encoding a protein, most commonly associated with antibiotic resistance or metabolic processes, bounded at its 3' end by an attC recombination site.^[23] The attC site, historically termed the 59-base element (59-be), consists of a conserved core sequence interrupted by variable loops that confer structural flexibility essential for site-specific recombination.^[24] These elements lack self-contained promoters in almost all cases, relying instead on the integron's housekeeping promoter (Pc) for expression when integrated.^[25] The diversity of gene cassettes is vast, with thousands sequenced to date, reflecting their role as hotspots for bacterial adaptation across environments.^[26] Prominent examples include the qacE cassette, which encodes an efflux pump conferring resistance to disinfectants like quaternary ammonium compounds, and the aadA cassette family, which provides protection against aminoglycoside antibiotics such as streptomycin and spectinomycin through adenylation.^[27] While many cassettes harbor known resistance determinants, many encode proteins of unknown function, underscoring the untapped potential of these elements beyond antimicrobial defense.^[23] Cassettes assemble into linear arrays within the integron platform through successive recombination events between attC sites and the attI integration site, enabling the accumulation of multiple functional units.^[23] These arrays commonly range from 1 to 10 cassettes in mobile integrons, though larger configurations occur in chromosomal contexts, allowing bacteria to maintain a repertoire of adaptive genes in a compact form.^[28] Recent discoveries highlight the non-resistance roles of cassettes, including anti-phage defense mechanisms. In 2025, analyses of chromosomal integrons revealed cassettes encoding streamlined anti-phage effectors, such as retron-based systems that trigger abortive infection to protect bacterial populations from viral predation.^[13] These findings expand the known functional spectrum of cassettes, illustrating their contribution to broader microbial resilience.

Mechanism of Action

Recombination Processes

The recombination processes in integrons are mediated by the integron-encoded integrase, a member of the tyrosine recombinase family, which catalyzes conservative site-specific recombination events between specific DNA sites. Integration of a gene cassette occurs through recombination between the integron's attI site and the cassette's attC site, resulting in the insertion of the cassette into the integron array in an oriented manner. Excision of a cassette, conversely, involves recombination between two attC sites, either adjacent ones to release a circular intermediate or between a cassette attC and the attI site for complete removal. These processes do not require Holliday junction resolution by additional enzymes, as the integrase performs both cleavage and religation steps using its conserved catalytic tyrosine residue.^[29] The recombination sites exhibit distinct structural features that influence their efficiency. The attI site is a compact, double-stranded sequence of approximately 65 base pairs (bp) located adjacent to the integrase gene, enabling high-efficiency recombination due to its stable, accessible conformation. In contrast, the attC site, carried by each gene cassette, is a longer sequence of about 59 bp with imperfect inverted repeats at its ends, which folds into a stem-loop structure, particularly in single-stranded form during replication, leading to lower recombination efficiency compared to attI. This structural variability in attC sites allows for diverse cassette integration but requires the integrase to bind and unfold the site for catalysis.^[29]^[30] Under normal cellular conditions, integration is favored over excision, maintaining cassette stability within the integron. However, excision rates increase dramatically during environmental stress, particularly through upregulation by the bacterial SOS response. The integrase promoter (Pint) is repressed by the LexA protein, which undergoes autocleavage during SOS induction, derepressing integrase expression and elevating cassette rearrangement rates by 100- to 1000-fold. This regulatory link ensures adaptive gene shuffling only when DNA damage signals heightened evolvability.^[31]^[32] While integrons themselves lack intrinsic mobility mechanisms, their dissemination via horizontal gene transfer (HGT) is facilitated by association with mobile elements such as transposons (e.g., Tn7-like elements) or conjugative plasmids, which excise and relocate the entire integron platform along with its cassettes between bacterial genomes.^[33]

Gene Expression and Regulation

In integrons, gene cassettes are typically promoterless and rely on the integron platform's Pc promoter for transcription, which initiates upstream of the attI site and produces a polycistronic mRNA encompassing the entire cassette array. This setup results in strong expression of proximal cassettes near the promoter, while distal cassettes exhibit weaker expression due to transcriptional polarity, often attributed to mRNA degradation or attenuation over distance. Cassette arrays in clinical integrons rarely exceed six elements, as further positions lead to negligible expression levels.^[20] The order and identity of cassettes significantly influence overall expression, with the first cassette modulating downstream levels more than mere distance from Pc; for instance, translation efficiency of upstream cassettes can repress or enhance subsequent ones, resulting in up to 120-fold variation in reporter gene fluorescence across different arrays. Some attC sites contain weak internal promoters that partially counteract polarity, boosting expression of downstream genes in cassettes like cmlA or qnrVC. Pc exists in variants such as weak (PcW), strong (PcS, ~17-fold stronger than PcW), hybrid 1 (~1.9-fold), and hybrid 2 (~4.6-fold), with activity differences spanning 10- to 20-fold and influencing cassette output accordingly.^[34]^[20]^[35] Integrase expression is regulated by the Pint promoter, which features LexA binding sites for autoregulation; under non-stress conditions, LexA represses Pint, limiting integrase to basal levels and preventing toxic hyperactivity, while SOS-inducible derepression allows up to 37-fold increases in response to DNA damage. This SOS response, triggered by antibiotics or conjugation, promotes cassette excision and shuffling without directly altering cassette transcription but enabling adaptive reconfiguration. Environmental stresses like subinhibitory antibiotics can tune cassette expression by stabilizing polycistronic mRNA, particularly for poorly translated distal elements, thereby enhancing resistance gene output during selective pressure.^[36]^[20]^[34]

Classification

Mobile Integrons

Mobile integrons are genetic elements borne on plasmids or transposons, enabling their dissemination through horizontal gene transfer in bacterial populations. These structures are classified into classes based on the sequence homology of their integrase genes (intI), with class 1 integrons (intI1) being the most predominant and clinically significant; specific class 1 variants are individually named, such as In0 or In2. They are particularly prevalent in Enterobacteriaceae such as Escherichia coli and Klebsiella pneumoniae. Unlike more stable genomic forms, mobile integrons facilitate the capture and expression of gene cassettes at the attI site, promoting adaptive responses in dynamic environments.^[37]^[9] Class 2 integrons (intI2) are less common, often found in Gram-positive bacteria and some Enterobacteriaceae, typically carrying fewer resistance genes like those encoding tetracycline or trimethoprim resistance. Class 3 (intI3) integrons are rare, mainly in Pseudomonas and Enterobacter species, associated with metallo-beta-lactamase genes. Classes 4 and 5 are even rarer, linked to specific mobile elements in environmental bacteria and occasional clinical isolates.^[4] Key characteristics of mobile integrons include compact arrays typically comprising 1 to 5 gene cassettes, which encode functional proteins under the control of an integron promoter. Their mobility is enhanced by association with conjugative plasmids or transposons like Tn402 derivatives, allowing efficient spread via bacterial conjugation at frequencies up to 10^{-3} per donor cell. Notably, around 70-80% of these integrons harbor antibiotic resistance genes, with sul1—conferring resistance to sulfonamides—being a conserved component in the 3' conserved segment of most class 1 variants, often fused with qacEΔ1 for additional biocide resistance. The core intI gene shows high conservation across these elements, supporting their recombinase activity.^[38]^[39]^[40] Representative examples illustrate their role in clinical settings. Tn21-like transposons, which integrate class 1 elements, are commonly found in E. coli isolates from human and animal sources, carrying cassettes such as aadA for aminoglycoside resistance. In clinical pathogens, mobile integrons contribute to multidrug resistance; for instance, approximately 46% of Pseudomonas aeruginosa isolates from hospital environments possess class 1 integrons with arrays including blaVIM-2 (carbapenem resistance) and aacA4 (aminoglycoside resistance). These structures amplify resistance dissemination in nosocomial infections.^[41]^[42] The evolution of mobile integrons involves recent mobilization from ancestral chromosomal sources, enabling rapid adaptation to selective pressures like antibiotics. Phylogenetic analyses indicate that class 1 integrons predate the antibiotic era but have undergone hybridization events, such as chimeric transposition modules in Tn6007-like elements, blending sequences from environmental mobile DNAs. A 2024 study highlighted genetic isolation between chromosomal and mobile forms, yet confirmed occasional cassette exchanges and hybrid formations that enhance resistance gene pools in pathogens.^[1]^[39]^[43]

Chromosomal Integrons

Chromosomal integrons, also known as sedentary integrons, represent stable genetic elements permanently integrated into bacterial chromosomes, distinguishing them from their mobile counterparts. These structures function as reservoirs for gene cassettes, enabling bacteria to capture and store diverse genetic material through site-specific recombination mediated by an integron-encoded integrase. Unlike smaller, transient integrons, chromosomal ones often form extensive arrays, sometimes exceeding 100 cassettes, which contribute to long-term genomic stability and adaptation in environmental niches.^[44]^[43] A hallmark of chromosomal integrons is their large size and functional diversity, encoding genes involved in metabolism, virulence, and other adaptive traits rather than primarily antibiotic resistance. For instance, in Vibrio cholerae, the VchIntIA integron on chromosome II comprises over 180 gene cassettes, many of which code for proteins with roles in environmental sensing and pathogenesis, such as sulfate-binding proteins and lipases. Similarly, in Shewanella amazonensis, the chromosomal integron SamIntIA supports cassette integration and excision, harboring arrays that enhance metabolic versatility in aquatic habitats. These integrons exhibit highly conserved attC recombination sites, facilitating precise cassette shuffling while maintaining overall array integrity. Their lower mobility stems from the absence of inherent transposition mechanisms, though rare mobilization can occur through association with insertion sequence (IS) elements that capture portions of the array for transfer to plasmids.^[45]^[46]^[44]^[1] Evolutionarily, chromosomal integrons trace back to ancient bacterial lineages, predating the emergence of mobile integrons by hundreds of millions of years and serving as progenitors for resistance-disseminating forms in γ-proteobacteria. Phylogenetic analyses indicate these elements coevolved with host genomes over 300–800 million years, with species-specific diversification in genera like Vibrio and Shewanella. Recent investigations, including a 2025 study, have revealed that many cassettes within these arrays encode compact anti-phage defense systems, such as retrons and toxin-antitoxin modules, positioning chromosomal integrons as biobanks for antiviral strategies that enhance bacterial survival against phage predation. The terminology "superintegron" historically described these expansive chromosomal arrays, reflecting their scale compared to mobile variants.^[44]^[47]

Distribution and Occurrence

Prevalence in Bacteria

Integrons are widely distributed across bacterial taxa, with a pronounced prevalence in Proteobacteria, where they are nearly ubiquitous. Surveys of sequenced genomes indicate that approximately 20% of γ-Proteobacteria harbor at least one complete integron, reflecting their role in diverse environmental and pathogenic species within this class. In contrast, integrons are less common in Gram-positive bacteria than in Proteobacteria but have been detected in up to 25% of isolates from animal-associated environments such as poultry litter. Although long considered absent in Archaea, recent genomic analyses have identified integrons in roughly 1% of archaeal metagenome-assembled genomes, primarily enriched in phyla like Asgardarchaeota, suggesting sporadic occurrence rather than broad distribution.^[48]^[49]^[50]^[51] Genomic surveys estimate that integrons are present in about 15% of all sequenced bacterial genomes, primarily detected through the integron integrase gene (intI). Among these, the majority are chromosomal integrons, often as large arrays in environmental bacteria like those in the Proteobacteria, while the remaining are mobile elements associated with plasmids or transposons. This distribution underscores the predominance of sedentary chromosomal forms in natural bacterial populations, with mobile variants more prominent in clinical settings.^[18]^[1] Metagenomic studies from 2023-2024 reveal integrons in roughly 30% of environmental samples, with elevated detection rates in soil microbiomes and wastewater communities compared to other habitats. These findings highlight integrons' persistence in complex microbial consortia, where they contribute to genetic diversity without direct ties to specific ecological pressures.^[52] Phylogenetic analyses of intI homologs suggest an ancient origin tracing back to the last bacterial common ancestor, with diversification driven by horizontal gene transfer across phyla. Class 1 integrons, characterized by the intI1 gene, dominate in pathogenic bacteria, comprising the majority of detected mobile forms in clinical isolates.^[18]^[53]

Environmental and Clinical Contexts

Integrons are particularly abundant in environmental niches impacted by anthropogenic pollution, such as river sediments near agricultural and aquaculture sites, where antibiotic runoff from livestock farming selects for their proliferation. For instance, in sediments from major lakes in China associated with aquaculture activities, class 1 integrons were detected in 80% of samples, class 2 in 100%, and class 3 in 46.67%, highlighting their elevated presence in areas with high antibiotic inputs from farms. This enrichment is driven by the selective pressure of antibiotics and heavy metals in runoff, which promote the capture and dissemination of resistance genes via integron gene cassettes.^[54] In clinical settings, integrons contribute significantly to the multidrug resistance of hospital pathogens, with prevalence rates often ranging from 40% to 70% in isolates from nosocomial infections. Among Acinetobacter baumannii strains, a common cause of hospital-acquired pneumonia and bloodstream infections, class 1 integrons have been reported in up to 52.8% of clinical isolates, facilitating the assembly of multiple resistance determinants that exacerbate outbreak dynamics in intensive care units. Their role in nosocomial outbreaks is evident in the rapid spread of resistant clones within healthcare facilities, where integron-mediated gene exchange enhances pathogen adaptability to antimicrobial therapies.^[55] Anthropogenic activities further amplify integron spread through hotspots like wastewater treatment plants (WWTPs), where incoming sewage concentrates these elements before partial removal during treatment. Class 1 integrons are among the most abundant mobile genetic elements in WWTP influents, persisting in effluents at reduced but detectable levels, thus serving as conduits for environmental release and potential recirculation into human-impacted ecosystems. This persistence underscores WWTPs as critical nodes in the global dissemination of integron-associated resistance.^[56] Globally, integron prevalence is notably higher in developing regions, where antibiotic misuse in agriculture, medicine, and aquaculture drives elevated selective pressures compared to stricter regulatory environments elsewhere. As of 2025, global surveillance indicates higher integron prevalence in low- and middle-income countries, linked to antibiotic use in agriculture.^[57]

Evolutionary and Adaptive Roles

Horizontal Gene Transfer

Integrons play a pivotal role in horizontal gene transfer (HGT) by enabling the dissemination of gene cassettes across bacterial populations, often through association with mobile genetic elements. Mobile integrons, particularly class 1 variants, are frequently carried on conjugative plasmids such as those in the IncP incompatibility group, which exhibit high transfer efficiencies during conjugation, reaching frequencies of approximately 10^{-2} per donor cell under optimal laboratory conditions.^[58] This process involves direct cell-to-cell contact via type IV secretion systems, allowing the plasmid, complete with its integron array, to be transferred from donor to recipient bacteria. Additionally, integrons can mobilize to new replicons through transposition, often facilitated by elements like Tn402 or Tn21, which excise and insert the entire integron structure into chromosomes or other plasmids, thereby expanding their propagation potential.^[10] Inter-species transfer is a hallmark of integron-mediated HGT, supported by their broad host range, particularly among Proteobacteria. For instance, integrons originating in environmental species such as Pseudomonas or Acinetobacter have been transferred to clinical pathogens like Escherichia coli or Salmonella, disseminating adaptive genes across ecological niches.^[59] Phylogenetic analyses of gene cassettes reveal mosaic patterns, with attC recombination sites indicating repeated cross-species exchanges, as evidenced by conserved cassette arrays shared among distantly related integron hosts despite divergent bacterial lineages.^[60] These transfers occur via multiple pathways, including natural transformation, where free DNA fragments containing integrons are taken up and integrated, achieving frequencies of 10^{-9} to 10^{-7} transformants per recipient in experimental settings.^[60] The efficiency of HGT via integrons allows the capture and expression of multi-cassette arrays, enabling simultaneous dissemination of multiple adaptive traits and enhancing population-level adaptation. However, this process faces barriers, notably restriction by CRISPR-Cas systems in recipient cells, which target incoming integron DNA and reduce acquisition of associated mobile elements, as observed in pathogens like Pseudomonas aeruginosa where CRISPR presence correlates with fewer integron markers.^[61] Evolutionarily, integrons function as "gene acquisition platforms," enabling bacteria to sample and rearrange cassettes rapidly; experimental evolution studies demonstrate that integrase activity accelerates resistance adaptation, with beneficial rearrangements emerging within hundreds to thousands of generations under selective pressure.^[8] This capability positions integrons as key drivers of bacterial diversification over 10^3 to 10^4 generations, far outpacing vertical inheritance alone.^[20]

Contributions to Antibiotic Resistance

Integrons significantly contribute to antibiotic resistance by serving as platforms for the capture, expression, and mobilization of diverse antibiotic resistance genes (ARGs) through their gene cassette arrays. These elements enable bacteria to assemble combinations of resistance determinants, conferring multidrug resistance phenotypes that complicate clinical treatments. In particular, mobile integrons, especially class 1 integrons, are prevalent in pathogenic Gram-negative bacteria and facilitate the rapid evolution of resistance under selective pressures from antibiotic exposure.^[9]^[11] The resistance cassette repertoire within integrons is remarkably diverse, encoding proteins such as efflux pumps (e.g., QacA, which expels quaternary ammonium compounds and disinfectants) and inactivating enzymes (e.g., bla_OXA β-lactamases that hydrolyze β-lactam antibiotics). These cassettes target over 10 distinct antibiotic classes, including aminoglycosides (via enzymes like AacA and AadA), β-lactams (via Bla_OXA and Bla_VIM), trimethoprim (via Dfr genes), and others such as chloramphenicol, fosfomycin, and rifampicin. A comprehensive profiling of integron-associated ARGs identified 136 unique open reading frames (ORFs), representing the majority of known resistance cassettes in clinical and environmental isolates, underscoring their role in generating combinatorial resistance profiles.^[11]^[9] Spread dynamics of integron-borne resistance are amplified by their association with conjugative plasmids and transposons, enabling horizontal gene transfer across bacterial populations. Class 1 integrons are detected in 20% to 60% of multidrug-resistant Enterobacteriaceae isolates from various clinical settings, depending on the study and location, such as Escherichia coli and Klebsiella pneumoniae, and have been implicated in global pandemics like the dissemination of the NDM-1 carbapenemase gene, which often co-resides with integron arrays carrying additional ARGs.^[62]^[63] This prevalence links environmental reservoirs to human pathogens, with estimates suggesting humans and animals excrete around 10²³ integron copies daily, perpetuating resistance cycles.^[64] Antibiotic use exerts strong evolutionary pressure on integrons, promoting their mobilization and cassette rearrangement via integrase activity, which is upregulated in response to sublethal antibiotic concentrations. This selection has led to the accumulation of diverse resistance ORFs, with 2023 analyses revealing 136 unique profiles that enhance bacterial adaptability. Interventions targeting integrons include phage therapy, where bacteriophages are engineered to disrupt integron-bearing plasmids or sensitize hosts to antibiotics, though challenges arise from the promiscuity of cassette exchange, allowing rapid adaptation and evasion of targeted therapies.^[11]^[9]

Other Adaptive Functions

Integrons facilitate metabolic adaptations in bacteria by capturing gene cassettes that encode enzymes for heavy metal detoxification, such as the mercury resistance mer operon components, which enable the reduction of toxic Hg²⁺ to less harmful elemental mercury via mercuric reductase activity.^[65] These cassettes are particularly prevalent in polluted environments, where integrons in metal-contaminated sites like mine tailings promote survival under selective pressures from anthropogenic heavy metals.^[66] Similarly, integron-associated cassettes contribute to xenobiotic degradation, with enzymes recovered from sediments in polluted bays (e.g., Suez and Tokyo Bays) that break down synthetic compounds like polychlorinated biphenyls and pesticides, allowing bacteria to utilize these pollutants as carbon sources.^[67]^[68] In pathogenic bacteria, integrons enhance virulence by incorporating toxin-antitoxin (TA) systems into their gene cassettes, which stabilize large arrays and regulate recombination to maintain adaptive gene diversity during host colonization.^[69] For instance, in Salmonella enterica, integrons carry TA modules that modulate persistence and fitness in infected tissues, contributing to systemic spread by countering host immune stresses without directly causing cell death under normal conditions.^[2] These systems, often overrepresented in chromosomal integrons of pathogens, ensure plasmid and array stability, indirectly boosting virulence factor expression.^[70] Recent studies have revealed integrons' role in anti-phage defense, with sedentary chromosomal integrons acting as reservoirs for compact cassettes encoding novel immunity systems, including retron-based and CRISPR-like mechanisms that trigger abortive infection to halt phage replication.^[47] In a 2025 analysis of bacterial genomes, approximately 20% of tested integron cassettes from arrays like those in Vibrio cholerae conferred anti-phage activity by inducing host cell lysis or growth arrest upon infection, protecting clonal populations in diverse environments.^[47] These defenses are streamlined for cassette integration, highlighting integrons' evolutionary utility beyond resistance. Ecologically, environmental integrons support niche colonization through cassettes promoting biofilm formation, such as those enhancing adhesion and extracellular matrix production in marine and sediment communities.^[71] In macroalgal biofilms, integron ORFs encoding signal peptides for membrane interactions (up to 11% of cassettes) facilitate attachment and community structuring, aiding bacteria in competing for resources on surfaces like Ulva and Sargassum.^[72] This enables persistent colonization in fluctuating habitats, where integron-mediated gene exchange in biofilms amplifies adaptive traits for survival.^[73]

Detection and Analysis

Molecular Techniques

Molecular techniques for detecting and characterizing integrons primarily rely on PCR amplification, sequencing, functional assays, and bioinformatics tools to identify key elements such as integrase genes, attC recombination sites, and gene cassette arrays. PCR-based methods are widely used for initial screening due to their specificity and sensitivity. Primers targeting the conserved integrase genes intI1, intI2, and intI3 enable detection of class 1, 2, and 3 integrons, respectively, in bacterial isolates or environmental samples.^[74] Multiplex PCR formats allow simultaneous amplification of these genes, facilitating rapid classification of integron types in diverse bacterial populations.^[75] For gene cassette arrays, multiplex PCR can target common resistance gene sequences within the variable region, though long arrays may require nested or semi-nested approaches to amplify complete structures.^[76] Quantitative real-time PCR variants achieve detection limits as low as 10² gene copies per milliliter, making them suitable for low-abundance samples.^[74] Sequencing approaches have advanced the resolution of integron structures, particularly for complex metagenomic contexts. Long-read technologies, such as PacBio, enable assembly of full-length cassette arrays by overcoming the limitations of short-read methods in resolving repetitive attC sites.^[77] Targeted amplification of integron regions followed by long-read sequencing has revealed underrepresented diversity in class 1 integrons from environmental microbiomes.^[77] Recent protocols from 2023, including amplicon sequencing of cassette arrays combined with metagenomic assembly, allow profiling of over 100 distinct cassettes per sample, enhancing functional annotation in microbial communities.^[78] Emerging CRISPR-Cas12a systems enable versatile, portable detection of integron-associated antibiotic resistance genes, such as blaCTX-M15 and floR, as demonstrated in 2025 protocols.^[79] Functional assays assess the mobility and activity of integrons in vivo. Conjugation mating-out experiments, involving donor-recipient bacterial pairs, evaluate the transfer frequency of integron-bearing plasmids or chromosomal elements, often under selective conditions to track mobilizable units.^[80] These assays confirm the role of integrons in horizontal gene transfer by quantifying transconjugants harboring intact cassette arrays. For integrase activity, reporter gene fusions—such as lacZ or gfp linked to the P_intI promoter—measure expression levels in response to environmental cues like antibiotics or stress, revealing regulatory dynamics without relying solely on genetic sequences.^[22] Bioinformatics tools complement experimental methods by automating annotation and prediction in genomic data. The INTEGRALL database serves as a comprehensive repository for integron sequences, integrases, and cassette arrangements, supporting homology-based searches and comparative analyses across bacterial genomes.^[81] Hidden Markov model (HMM)-based approaches, like those in HattCI or IntegronFinder, detect attC sites with high accuracy by modeling their conserved secondary structures, enabling de novo identification in unannotated metagenomes.^[82] These models prioritize sequence motifs essential for recombination, improving the specificity of integron boundary delineation over simple pattern matching.^[83]

Challenges and Advances

One major challenge in integron research is the anonymity of gene cassettes, where approximately two-thirds (64%) of open reading frames (ORFs) remain of unknown function, complicating efforts to understand their biological roles and contributions to bacterial adaptation.^[84] This issue arises from the vast diversity of cassettes captured by integrons, many of which encode hypothetical proteins without clear homologs in databases, hindering functional annotation. Additionally, short-read sequencing technologies often lead to assembly errors when reconstructing integron arrays, as repetitive attC sites and variable cassette compositions cause misalignments and chimeric contigs.^[85] These errors are particularly problematic in complex metagenomic samples, where distinguishing true cassette arrays from artifacts proves difficult. Another limitation is the low excision rates of cassettes in vitro, attributed to the inefficient recombination mediated by the integron integrase on single-stranded attC substrates, which occurs at frequencies orders of magnitude lower than in vivo conditions.^[30] Recent advances have addressed these challenges through innovative tools. In 2025, CRISPR-Cas systems were adapted for precise editing of integron arrays, enabling functional validation of cassettes by targeted insertion, excision, or disruption in bacterial hosts, thus overcoming limitations in natural recombination efficiency.^[86] Complementing this, AI-driven algorithms have improved predictions of attC site folding and recombination potential, using machine learning to model secondary structures based on sequence features and thermodynamic parameters, which enhances cassette identification accuracy in genomic data.^[87] These methods facilitate higher-resolution analysis of integron dynamics without relying solely on empirical recombination assays. Looking ahead, future directions include integration of integron data with pangenomics approaches will further enable evolutionary tracing by mapping cassette distributions across bacterial pangenomes, revealing patterns of horizontal transfer and adaptation.^[1] However, engineering resistance integrons raises ethical concerns regarding dual-use risks, as advancements in cassette manipulation could inadvertently facilitate the spread of antibiotic resistance in clinical and environmental settings.^[88]

References

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Integrons: Past, Present, and Future - ASM Journals
May 20, 2014 · There are five classes of “mobile” integrons, all associated with antibiotic resistance: classes 1, 2, and 3, usually recovered from clinical ...
[2]
The Integron: Adaptation On Demand | Microbiology Spectrum
Integrons are genetic platforms that allow bacteria to evolve rapidly through the acquisition, stockpiling, excision, and reordering of open reading frames.
[3]
Full article: Integrons
Integrons are genetic elements that contain a site-specific recombination system able to integrate, express and exchange specific DNA elements, called gene ...
[4]
Resistance integrons: class 1, 2 and 3 integrons - PMC
Oct 20, 2015 · Up to date, class 1 integrons have been reported on Gram-positive bacteria including Corynebacterium, Streptococcus, Enterococcus, ...
[5]
Predicting the taxonomic and environmental sources of integron ...
Aug 9, 2021 · Integrons are bacterial genetic elements that can insert and excise mobile gene cassettes by site-specific recombination. They are mostly known ...Missing: definition | Show results with:definition
[6]
Vehicles and pathways for horizontal dissemination in bacteria - PMC
Integrons are genetic elements that contain a site-specific recombination system able to integrate, express and exchange specific DNA elements, called gene ...
[7]
Facilitators of antimicrobial resistance and niche adaptation
Nov 17, 2023 · Integrons can thus provide traits that facilitate host cells to survive, adapt, and interact with their surrounding environment, and provide the ...
[8]
Integron activity accelerates the evolution of antibiotic resistance
Feb 26, 2021 · In summary, integrons accelerate resistance evolution by rapidly generating combinatorial variation in cassette composition while maintaining ...
[9]
Integrons in the development of antimicrobial resistance - Frontiers
Integrons play a central role in disseminating and assembling genes connected with antibiotic resistance in pathogenic and commensal bacterial species.
[10]
Integrons in the development of antimicrobial resistance
Aug 31, 2023 · Integrons play a central role in disseminating and assembling genes connected with antibiotic resistance in pathogenic and commensal bacterial species.
[11]
Profile and resistance levels of 136 integron resistance genes - Nature
Oct 11, 2023 · Integrons have played a major role in the rise and spread of multidrug resistance in Gram-negative pathogens and are nowadays commonplace ...
[12]
https://www.cell.com/trends/microbiology/fulltext/S0966-842X(19)30317-8
[13]
Mobile integrons encode phage defense systems - Science
May 8, 2025 · Integrons that are located in bacterial chromosomes also act as biobanks for antiphage defense cassettes, often streamlined to fit size constraints.
[14]
Integron and its role in antimicrobial resistance: A literature review ...
Of all the different classes of integrons, the class I integrons and then class II are the most common classes among clinical isolates (6). Classes I to III are ...
[15]
A novel family of potentially mobile DNA elements encoding site ...
A novel family of potentially mobile DNA elements encoding site-specific gene-integration functions: integrons. H. W. Stokes, ... First published: December 1989.
[16]
Class 1 integrons, gene cassettes, mobility, and epidemiology
Integrons are genetic elements that, although unable to move themselves, contain gene cassettes that can be mobilized to other integrons or to secondary sites ...
[17]
DNA sequence of both chromosomes of the cholera pathogen Vibrio ...
Aug 1, 2000 · This genomic sequence of V. cholerae confirmed the presence of a large integron island (a gene capture system) located on chromosome 2 (125.3 ...
[18]
Integrons in the Age of Antibiotic Resistance: Evolution, Mechanisms ...
Dec 13, 2024 · Integrons, which are genetic components commonly found in bacteria, possess the remarkable capacity to capture gene cassettes, incorporate them ...Missing: definition | Show results with:definition
[19]
The SOS response controls integron recombination - PubMed
May 22, 2009 · We report a direct link between this system and the ubiquitous SOS response. We found that LexA controlled expression of most integron integrases.
[20]
Integrons: Past, Present, and Future - PMC - PubMed Central
Integrons are versatile gene acquisition systems commonly found in bacterial genomes. They are ancient elements that are a hot spot for genomic complexity.
[21]
Integron Functionality and Genome Innovation: An Update on the ...
Integrons are versatile genetic platforms involved with the acquisition, stockpiling, excision, and rearrangements of gene cassettes by site-specific ...2.1. 2. Pc Promoter In Class... · 2.1. 3. Pc Promoter In Class... · 2.1. 4. Pc Promoter In...<|control11|><|separator|>
[22]
Gene Expression in Class 2 Integrons Is SOS-Independent and ...
Aug 14, 2017 · Thus, integrase expression is not regulated by the SOS response in class 2 integrons, contrary to what has been shown for class 1 and V.
[23]
Gene cassettes and cassette arrays in mobile resistance integrons
intI1, intI2 and intI3 were first identified in association with mobile genetic elements and intI4 and others with chromosomal integrons. However, intI1 ...Capture Of Cassettes And... · Integrons With Inti1 · Gene Cassette Nomenclature<|control11|><|separator|>
[24]
Structural Features of Single-Stranded Integron Cassette attC Sites ...
The attI sites contain a degenerate core recombination site and, in the specific case ... L′ and L″ are inverted repeat sequences that show little sequence ...
[25]
Diversity and strength of internal outward-oriented promoters in ...
Almost all gene cassettes are promoterless structures that depend on the Pc promoter to express their genes. Among class 1 integrons, several Pc variants (the ...
[26]
Integrons and gene cassettes: hotspots of diversity in bacterial ...
Sep 6, 2012 · Cassettes usually contain only one gene, potentially any gene, and an attC recombination site, and thousands of cassettes have been sequenced.
[27]
Resistance integrons: class 1, 2 and 3 integrons
Oct 20, 2015 · Via specific excision and integration, gene cassettes are integrated between two recombination sites (attI and attC) and thus become part of the ...Class 1 Integron · Class 2 Integron · Integrons In Food Borne...
[28]
Gene cassette transcription in a large integron-associated array - PMC
Sep 15, 2010 · Cassette arrays can vary in length from lone integrons and the typically short (1-8 cassettes) antibiotic resistant arrays seen associated ...
[29]
Efficiency of Recombination Reactions Catalyzed by Class 1 ... - PMC
Core site sequences and the recombination crossover point are marked as for panel A. The reactions catalyzed by the IntI1 integrase encoded by class 1 integrons ...
[30]
Integron cassette insertion: a recombination process ... - EMBO Press
A novel integron cassette insertion model in which the single bottom attC strand adopts a folded structure generating a double strand recombination site.Results · Inti1 In Vitro Binding... · λ Phage Attb × Attp...
[31]
The SOS Response Controls Integron Recombination - Science
May 22, 2009 · We report a direct link between this system and the ubiquitous SOS response. We found that LexA controlled expression of most integron integrases.
[32]
Conjugative DNA Transfer Induces the Bacterial SOS Response and ...
We used lacZ and gfp fusions to address whether conjugation induces the SOS response and the integron integrase. ... 100 fold to 1000 fold higher rate than under ...
[33]
Cargo Genes of Tn7-Like Transposons Comprise an Enormous ...
Dec 7, 2021 · Examination of the cargo carried by Tn7 and about 50 other Tn7-like transposons identified integrons with antibiotic resistance gene cassettes, ...
[34]
The expression of integron arrays is shaped by the translation rate of ...
Oct 25, 2024 · Given their high mobility through HGT ... Diversity of class 1 integron gene cassette rearrangements selected under antibiotic pressure.<|control11|><|separator|>
[35]
Relative Strengths of the Class 1 Integron Promoter Hybrid 2 and the ...
There are four versions of Pc, designated “weak,” “strong,” “hybrid 1,” and “hybrid 2,” exhibiting differences in the −35 and/or −10 sequences, separated by 17 ...
[36]
Unbridled Integrons: A Matter of Host Factors - PMC - PubMed Central
Mar 8, 2022 · The stable platform of the integron contains (i) the integrase gene (intI) under the control of its promoter Pint, (ii) the attI integration ...
[37]
Genetic characterization of novel class 1 Integrons In0, In1069 and ...
Aug 22, 2017 · This study aims to characterize genetically related class 1 integrons In1069, In893 and In1287 to In1290, and to further propose a scheme of stepwise ...Bacterial Isolates And... · Integron Cloning And... · Genetic Futures Of Novel In0
[38]
Resistance integrons and super-integrons - ScienceDirect.com
Presence of a group II intron in a multiresistant Serratia marcescens strain that harbors three integrons and a novel gene fusion. Antimicrob Agents ...
[39]
A Class 1 Integron Present in a Human Commensal Has a Hybrid ...
In a survey of class 1 integrons from human stools, an unusual class 1 integron from a strain of Enterobacter cloacae was isolated and characterized in detail.
[40]
Integrons and gene cassettes: a genetic construction kit for bacteria
A comprehensive genetic engineering tool kit that provides the potential to remodel and to mix and match resistance genes according to requirements.
[41]
Incidence and Characterization of Integrons, Genetic Elements ... - NIH
The integron acquires or exchanges antibiotic resistance genes with its specialized recombinase, intI (11, 38, 59). These genetic elements are responsible for ...
[42]
Occurrence and Characteristics of Class 1 and 2 Integrons in ... - NIH
Class 1 integrons were commonly found in the tested P. aeruginosa isolates (45.8%, 54/118), but the class 1 integron-positive rates had been decreasing during ...
[43]
The evolutionary history of chromosomal super-integrons ... - PNAS
Integrons are genetic elements that acquire and exchange exogenous DNA, known as gene cassettes, by a site-specific recombination mechanism.<|control11|><|separator|>
[44]
Chromosomal integrons are genetically and functionally isolated ...
In this work, we have explored the connection of SCIs to their host genome using as a model the Superintegron (SI), a 179-cassette long SCI in the genome of ...
[45]
Cassette recruitment in the chromosomal Integron of Vibrio cholerae
Integrons confer a rapid adaptation capability to bacteria. Integron integrases are able to capture and shuffle novel functions embedded in cassettes.
[46]
Analysis by Mutagenesis of a Chromosomal Integron Integrase from ...
The aim of this study was to analyze the capacity of the chromosomal integron integrase SamIntIA of the environmental bacterium Shewanella amazonensis SB2BT to ...
[47]
Sedentary chromosomal integrons as biobanks of bacterial ...
May 8, 2025 · Integrons that are located in bacterial chromosomes also act as biobanks for antiphage defense cassettes, often streamlined to fit size constraints.Missing: gene cassettes
[48]
Identification and analysis of integrons and cassette arrays in ...
We used two criteria to class integrons: frequency within the species' genomes (Supplementary Figure S3) and number of cassettes (Supplementary Figure S4).INTRODUCTION · MATERIALS AND METHODS · RESULTS · DISCUSSION
[49]
Gram-positive bacteria are a major reservoir of Class 1 antibiotic ...
Using traditional cultivation and cultivation-independent molecular techniques, we quantified antibiotic resistance genes and mobile elements called integrons ...
[50]
Prevalence and characterization of integrons from bacteria isolated ...
Oct 2, 2007 · Class 1 integrons were present in 3.8% of Gram-positive and Gram-negative isolates from environments polluted by quaternary ammonia compounds.Materials And Methods · Integron Screening By... · Results<|separator|>
[51]
Discovery of integrons in Archaea: Platforms for cross-domain gene ...
Nov 16, 2022 · Here, we present the first evidence of integrons in the domain Archaea. We demonstrate that they have the same functional characteristics as ...
[52]
Detecting Class 1 Integrons and Their Variable Regions in ... - NIH
However, analysis of the relative abundance revealed that integrons were present in approximately 30% of environmental and animal samples, while 25% of human ...
[53]
Conserved phylogenetic distribution and limited antibiotic resistance ...
Jul 21, 2018 · Integrons, especially the class 1 integrons, are major contributors to the acquisition and dissemination of antibiotic resistance genes (ARGs).
[54]
https://doi.org/10.1016/j.envpol.2024.123709
[55]
Prevalence and Characterization of Class I Integrons among ... - NIH
Class I integrons were detected in 40.8% (40/98) of Pseudomonas aeruginosa strains and 52.8% (56/106) of Acinetobacter baumannii strains in the Nanjing area ...
[56]
Impact of Wastewater Treatment on the Prevalence of Integrons and ...
Our results showed that class 1 integrons were most abundant in WWTPs and that wastewater treatment significantly reduced the abundance of all integrons.Missing: post- | Show results with:post-
[57]
IncP plasmids are most effective in mediating conjugation between ...
The conjugative system of the IncPα plasmids was shown to be most effective in conjugative transfer, giving peak values of (2.7 ± 0.2) × 10−2 S. lividans TK24 ...<|control11|><|separator|>
[58]
Horizontal gene transfer in plant microbiomes: integrons as hotspots ...
Apr 28, 2024 · Integrons are genetic elements recently shown to be abundant in plant microbiomes, and are associated with HGT across broad phylogenetic boundaries.
[59]
Natural Transformation Facilitates Transfer of Transposons ...
Here we have investigated the potential for transposons, integrons and gene cassettes, supplied as fragmented DNA substrates, to move horizontally between ...Missing: replicons | Show results with:replicons
[60]
CRISPR-Cas is associated with fewer antibiotic resistance genes in ...
Nov 29, 2021 · We modelled the association between CRISPR-Cas and indicators of HGT, and found that pathogens with a CRISPR-Cas system were less likely to carry ABR genes.
[61]
Prevalence of Class I Integrons among Multidrug-resistant Gram ...
Nov 26, 2024 · Class I integrase gene distribution among multidrug resistant bacteria was 80% (24/30) in comparison to non-multidrug resistant bacteria 43.34% ...
[62]
Detection of New Delhi metallo-beta-lactamase enzyme gene bla ...
Organisms possessing the bla NDM-1 gene (responsible for carbapenem resistance) with a class-1 integron can acquire many other antibiotic resistance genes ...
[63]
Detection of Antimicrobial and Heavy-Metal Resistance Genes in ...
Jul 23, 2021 · The mer operon can carry integrons with one or more antimicrobial resistance gene cassettes. Therefore, merA gene can be distributed by Hg ...
[64]
Integron Diversity in Heavy-Metal-Contaminated Mine Tailings and ...
Integrons are horizontal gene transfer (HGT) systems containing elements necessary for site-specific recombination and expression of foreign DNA.Missing: mer | Show results with:mer
[65]
https://www.liebertpub.com/doi/10.1089/mdr.2020.0590
[66]
https://pmc.ncbi.nlm.nih.gov/articles/PMC348930/
[67]
Cassette recombination dynamics within chromosomal integrons are ...
Jan 12, 2024 · To simplify the results, we grouped together SCIs and CALINs, without distinction, under the global term SCIs. ... superintegron. J. Bacteriol.Missing: terminology | Show results with:terminology
[68]
Biological Functions of Type II Toxin-Antitoxin Systems in Bacteria
The Salmonella virulence plasmid pSLT (50–90-kb) possesses two type II TA systems, vapBC and ccdAB, and both are found to contribute to the stability of this ...
[69]
The function of integron-associated gene cassettes in Vibrio species
The class 1 integron was the first described integron (Stokes and Hall, 1989), found linked to antibiotic resistance genes in resistance plasmids from Gram ...
[70]
Microbial biofilms on macroalgae harbour diverse integron gene ...
We demonstrated that integrons in microbial biofilms did not sample genes randomly from the surrounding seawater, but harboured specific functions.
[71]
The Stringent Response Promotes Antibiotic Resistance ...
Aug 16, 2016 · Integrase expression is regulated by the bacterial SOS response (12). This coordinated response to DNA damage requires a repressor, LexA ...
[72]
Quantitative multiplex real-time PCR for detecting class 1, 2 and 3 ...
We developed a specific and sensitive Taqman® probe-based real-time PCR method with three different primer–probe pairs for simultaneous detection of the three ...
[73]
Multiplex PCR for screening of integrons in bacterial lysates
Integron classes were determined by multiplex PCR. The sequences of primers targeting intI1, intI2 and intI3 integrase genes were recommended by Dillon et al. ( ...
[74]
https://academic.oup.com/jac/article/65/8/1642/736734
[75]
Underrepresented high diversity of class 1 integrons in the ...
Sep 15, 2021 · To analyze thousands of ARGs in a high-throughput way, standardized and integrated pipelines are needed. The new version (v3.0) of the ...<|control11|><|separator|>
[76]
Functional enrichment of integrons: Facilitators of antimicrobial ... - NIH
Oct 24, 2023 · Integrons are genetic elements, found among diverse bacteria and archaea, that capture and rearrange gene cassettes to rapidly generate genetic diversity and ...
[77]
Integron Mobilization Unit as a Source of Mobility of Antibiotic ... - NIH
We describe here a novel genetic structure, named the integron mobilization unit (IMU), that has characteristics similar to those of miniature inverted ...
[78]
a database and search engine for integrons, integrases and gene ...
INTEGRALL is a freely available, text-based search system developed with the aim of collecting and organizing information on integrons in a single database.
[79]
HattCI: Fast and Accurate attC site Identification Using Hidden ...
In this article, we present HattCI, a new method for fast identification of attC sites in DNA sequence data based on hidden Markov model (HMM). The method ...
[80]
Identification and analysis of integrons and cassette arrays in ...
Integrons recombine gene arrays and favor the spread of antibiotic resistance. Their broader roles in bacterial adaptation remain mysterious.
[81]
A comprehensive survey of integron-associated genes present in ...
Jul 20, 2020 · Integrons present in pathogenic bacteria often carry antibiotic resistance genes, which enable the bacteria to survive antibiotic treatment. ...
[82]
Methods for the targeted sequencing and analysis of integrons ... - NIH
Integrons are microbial genetic elements that can rapidly generate genetic diversity by inserting and rearranging modular, mobilisable genes known as gene ...
[83]
[PDF] Exploring Clinical Class 1 Integrons as Valuable Targets for the Re ...
Mar 28, 2025 · Mobile genetic elements and class 1 integrons are important facilitators for antibiotic resistance genes, with the latter being highly conserved ...
[84]
Predicting algorithm of attC site based on combination optimization ...
Jun 21, 2022 · presented a new method HattCI based on Hidden Markov Model (HMM) to quickly and accurately identify attC sites in DNA sequence data. This model ...
[85]
3D immuno-RNA/DNA FISH in T cells
May 3, 2023 · We present a detailed protocol to simultaneously detect nascent RNA transcripts (3D RNA FISH), their genomic loci (3D DNA FISH) and/or their chromosome ...Missing: integron | Show results with:integron
[86]
Antibiotic Resistance and Dual Use - Online Ethics Center
What are the similarities and differences in considerations of dual use as it applies to engineers, agricultural scientists, microbiologists, medical ...Missing: issues integrons