The rhomboid fossa, also known as the floor of the fourth ventricle, is a diamond-shaped depression in the posterior aspect of the brainstem formed by the dorsal surfaces of the pons and the open portion of the medulla oblongata.[1] It serves as the ventral wall of the fourth ventricle, a cerebrospinal fluid-filled cavity in the hindbrain, and is lined by ependymal cells.[1] This structure is critical in neuroanatomy due to its role in housing several cranial nerve nuclei and providing a landmark for brainstem organization.[2]The rhomboid fossa exhibits a symmetrical, rhomboidal outline, typically measuring approximately 2-3 cm in length and 1-2 cm in width, with its superior apex at the pontomedullary junction and inferior apex near the obex of the fourth ventricle.[3] It is divided into three main parts: an upper triangular portion corresponding to the pons, an intermediate rhomboidal section, and a lower triangular area from the medulla.[1] A prominent median sulcus runs longitudinally down its center, dividing it into left and right halves and featuring the median eminence, which includes elevations such as the facial colliculus (formed by the genu of the facial nerve looping over the abducens nucleus) and the hypoglossal and vagal trigones.[2] Lateral to the sulcus limitans, the vestibular area contains nuclei associated with balance and eye movements.[1]Transverse striae medullares, strands of fibers from the arcuate nuclei, cross the upper part of the fossa, distinguishing the pontine from the medullary regions and contributing to cerebellar connections via the arcuatocerebellar tract.[3] The locus coeruleus, a cluster of noradrenergic neurons, is located in the lateral floor of the rhomboid fossa, playing a key role in arousal and stress responses.[1] Embryologically, the rhomboid fossa develops from the dorsal wall of the neural tube in the metencephalon and myelencephalon during the fifth week of gestation, thinning to form the ventricular floor as the hindbrain segments.[2] Its anatomical landmarks are essential for surgical approaches in brainstem procedures, guiding safe entry zones while avoiding critical nuclei and tracts.[3]
Overview
Definition
The rhomboid fossa is a diamond- or rhombus-shaped depression that forms the floor of the fourth ventricle, a cerebrospinal fluid-filled cavity in the hindbrain.[4] This structure lies on the dorsal surfaces of the pons and medulla oblongata, providing a key anatomical landmark in the brainstem.[1]In adults, the rhomboid fossa measures approximately 3.5 cm in length and 2.3 cm in width, though these dimensions can vary slightly between individuals.[5] The term "rhomboid" derives from the Greek rhombos, referring to its rhombus-like geometric form, while "fossa" comes from the Latin word for a trench or depression, aptly describing its shallow, pitted appearance.[6]The rhomboid fossa consists primarily of a thin layer of gray matter, continuous with that of the medulla oblongata and underlying the nuclei of several cranial nerves, and is covered by a delicate ependymal epithelium that lines the ventricular system.[7] This ependyma facilitates the circulation of cerebrospinal fluid within the fourth ventricle.[6]
Location and relations
The rhomboid fossa is situated in the posterior aspect of the pons and the open medulla oblongata, forming the floor of the fourth ventricle.[1] It appears as a diamond-shaped depression on the dorsal surface of these brainstem structures, extending superiorly from the cerebral aqueduct to the obex inferiorly.[8] This positioning places the fossa centrally within the posterior cranial fossa, contributing to the ventricular system's architecture for cerebrospinal fluid circulation.[6]The rhomboid fossa features the sulcus limitans laterally, a longitudinal groove that separates the medial eminence—containing motor nuclei—from the adjacent vestibular and cochlear areas overlying sensory nuclei.[9] The sulcus limitans runs parallel to the median sulcus along the length of the fossa, delineating these functional zones.[7]Superiorly, the rhomboid fossa is continuous with the aqueduct of Sylvius (cerebral aqueduct), providing a conduit for cerebrospinal fluid from the third ventricle.[1] Inferiorly, it transitions at the obex into the central canal of the spinal cord, marking the caudal limit of the fourth ventricle.[8] Surrounding structures include the roof of the fourth ventricle, formed by the superior and inferior medullary vela attached to the cerebellum and incorporating choroid plexus for cerebrospinal fluid production; anteriorly, the fossa relates to the basilar artery via the ventral brainstem tissue.[6][2]
Anatomy
Overall structure
The rhomboid fossa constitutes the diamond-shaped floor of the fourth ventricle, formed by the dorsal surfaces of the pons and the open portion of the medulla oblongata. It exhibits a tripartite division along its rostrocaudal extent, comprising a superior part corresponding to the pontine recess, an intermediate part in the medullary portion, and an inferior part forming the closed medulla triangle. This arrangement reflects the underlying brainstem segmentation, with the superior part being triangular and positioned rostrally, the intermediate part representing the broadest central region, and the inferior part tapering to a caudal apex.[1]The central axis of the rhomboid fossa aligns along the midline, facilitating symmetrical neural organization, while lateral expansions occur at the recesses to accommodate adjacent structures such as the cerebellar peduncles. Its surface appearance features a relatively smooth central area interspersed with elevated regions, including trigones and colliculi, which contribute to the fossa's characteristic rhomboidal contour. As the floor of the fourth ventricle, it provides a critical interface for cerebrospinal fluid circulation and houses key brainstem nuclei.[1][2]Vascular supply to the rhomboid fossa derives primarily from the vertebrobasilar system, with branches from the posterior inferior cerebellar artery (PICA) perfusing the lateral aspects of the medullary components and the anterior spinal artery providing medial supply to the inferior regions. In the pontine portion, additional contributions come from paramedian branches of the basilar artery and circumferential branches of the anterior inferior cerebellar artery, ensuring robust oxygenation to the underlying neural tissue. These arterial distributions are essential for maintaining the metabolic demands of the densely packed nuclei and tracts within the fossa.[10][2]
Parts and boundaries
The rhomboid fossa is divided into three distinct parts: superior, intermediate, and inferior, forming its characteristic diamond-shaped floor within the fourth ventricle.[1]The superior part is triangular in shape, extending from the opening of the cerebral aqueduct superiorly to the level of the pontine flexure inferiorly, and is bounded laterally by the superior cerebellar peduncles.[11] This region, formed by the dorsal surface of the pons, measures approximately 1 cm in length.[12]The intermediate part assumes a rhomboidal configuration, spanning the area between the pontomedullary junction superiorly and the obex inferiorly, and features lateral recesses that open into the foramina of Luschka.[7] It is limited laterally by the middle cerebellar peduncles and measures about 1.5 cm in length.[12]The inferior part is a triangular area known as the calamus scriptorius, with its base adjacent to the obex and apex directed downward into the central canal of the medulla oblongata, measuring roughly 0.5 cm in length.[11][12]Overall, the rhomboid fossa is bounded superiorly by the cerebral aqueduct, inferiorly by the obex, laterally by the foramina of Luschka within the lateral recesses, and centrally by the medial eminence flanking the median sulcus.[1][7]
Floor features
The floor of the rhomboid fossa, forming the posterior surface of the pons and upper medulla oblongata, presents a series of distinct elevations, depressions, and grooves that serve as surface landmarks for underlying neural structures.[1] These features are divided by the median sulcus into symmetrical halves, with the sulcus limitans running laterally to separate motor and sensory regions.[1]The facial colliculus appears as an oval elevation in the pontine portion of the floor, situated in the medial eminence opposite the superior fovea.[1] It is formed by the motor fibers of the facial nerve (cranial nerve VII) looping dorsally around the abducent nucleus (cranial nerve VI) before descending to exit the brainstem.[13] This prominence lies within the superior part of the rhomboid fossa, providing a key rostral landmark.[13]In the medullary portion, the hypoglossal trigone manifests as a longitudinal elevation medial to the sulcus limitans, overlying the hypoglossal nucleus.[1] Adjacent and lateral to it lies the vagal trigone, a triangular depression or elevation that covers the dorsal motor nucleus of the vagus nerve.[1] The vagal trigone, also known as the ala cinerea, extends from the calamus scriptorius at its base to an apex near the acoustic striae, with its medial third housing the dorsal motor nucleus and the lateral two-thirds the nucleus of the solitary tract.[14]At the caudal tip of the rhomboid fossa, near the obex, the area postrema emerges as a paired, highly vascularized prominence bounded superiorly by the funiculus separans and inferiorly by the gracile tubercle.[15] This structure, composed of ependymal cells, glia, neurons, and fenestrated capillaries, lacks a typical blood-brain barrier, functioning as a circumventricular organ.[15]The sulcus limitans constitutes a shallow longitudinal groove along the lateral margin of the medial eminence, delineating the boundary between motor (medial) and sensory (lateral) domains of the floor.[1] It widens superiorly into the superior fovea, a small dimple at the level of the facial colliculus, and inferiorly into the inferior fovea, a depression marking the transition to the medullary region.[1] These foveae serve as subtle indentations highlighting the positions of underlying cranial nerve nuclei.[1]Crossing the intermediate portion of the floor are the striae medullares, fine transverse bundles of arcuate fibers from the arcuate nuclei of the medulla oblongata that extend obliquely from the vestibular area toward the median sulcus.[1] These strands divide the rhomboid fossa into upper and lower triangles, contributing to its characteristic diamond shape.[1]
Histology
Epithelial covering
The rhomboid fossa, forming the floor of the fourth ventricle, is lined by a layer of ependymal cells that constitute a simple ciliated columnar epithelium derived from neuroepithelial precursors. These cells feature apical cilia and microvilli that facilitate the directional propulsion of cerebrospinal fluid (CSF) across the ventricular surface, aiding in its circulation within the central nervous system. The ependymal layer maintains a uniform, single-cell thickness typically ranging from 10 to 20 micrometers, providing a protective interface between the CSF and underlying neural tissue.In specialized regions such as the area postrema, located at the caudal aspect of the rhomboid fossa, the ependymal covering transitions to a thinner, modified form composed of flattened tanycyte-like cells with reduced ciliature. This region overlies fenestrated capillaries that enable direct exchange with the bloodstream, distinguishing it from the standard ependymal barrier. The ependyma of the rhomboid fossa is continuous with the epithelial lining of the adjacent choroid plexus, where similar ciliated cells contribute to CSF production and dynamics.The ependymal covering serves as a selective permeability barrier, forming tight junctions that regulate the passage of ions, nutrients, and metabolites between the CSF and brain parenchyma, thus contributing to the blood-CSF barrier. However, at circumventricular organs like the area postrema, this barrier is attenuated, permitting the detection of circulating hormones and toxins while restricting broader parenchymal access through specialized tanycytic processes.
Underlying neural elements
The underlying neural elements of the rhomboid fossa consist primarily of the tegmental gray matter from the pons and upper medulla oblongata, which forms the dorsal aspect of the brainstem and is rich in neuronal cellbodies organized into various nuclei.[1] This gray matter lies immediately beneath the ependymal lining and includes clusters of neurons that integrate sensory and motor functions essential to brainstem operations.[2]Glial elements, including astrocytes and oligodendrocytes, provide structural and metabolic support to this region, with astrocytes contributing to the blood-brain barrier integrity around subependymal areas and oligodendrocytes facilitating myelination of adjacent tracts.[1] These glial cells are particularly prominent in areas like the area postrema, where they form a specialized, highly vascular interface lacking a typical blood-brain barrier.[1]Key nuclear groups embedded within the floor include the vestibular nuclei (superior, medial, lateral, and inferior), which occupy the vestibular area and process balance-related inputs; the cochlear nuclei (dorsal and ventral), located at the pontomedullary junction and forming the acoustic tubercle; and the nucleus of the solitary tract, which spans the medullary portion and handles visceral sensory afferents.[2][2][14] These nuclei are situated deep to the ependyma, with their positions corresponding to surface landmarks such as the vestibular area and vagal trigone.[14]Connectivity in this region involves fibers from several cranial nerves that either loop through or pierce the floor en route to their nuclei. For instance, facial nerve (CN VII) fibers loop dorsally around the abducens nucleus within the facial colliculus before exiting; vestibulocochlear nerve (CN VIII) afferents terminate directly in the vestibular and cochlear nuclei; glossopharyngeal (CN IX) and vagus (CN X) fibers project to the solitary nucleus for sensory relay; and hypoglossal nerve (CN XII) rootlets originate from the hypoglossal nucleus and course ventrally, piercing the medullary tegmentum.[2][2][14][1]
Development
Embryonic origins
The rhomboid fossa originates from the hindbrain region of the embryonic neural tube, specifically within the rhombencephalon, which is transiently segmented into eight rhombomeres (r1 to r8). These rhombomeres represent compartmentalized units that establish the anteroposterior patterning of the hindbrain, with the rhomboid fossa forming as the ventral midline floor of the emerging fourth ventricle anlage. The dorsal closure of the neural tube in the hindbrain region during the fourth week of gestation sets the stage for this segmentation, where each rhombomere contributes to distinct neuronal populations underlying the fossa's future structures.[16]The development of the rhomboid fossa's ventral midline is critically induced by signals from the notochord and the floor plate of the neural tube. The notochord, a midline mesodermal structure, secretes Sonic hedgehog (Shh) protein, which induces the overlying neural plate cells to differentiate into floor plate cells along the ventral midline of the rhombencephalon. These floor plate cells then amplify Shh signaling, creating a concentration gradient that patterns ventral cell fates, including the ependymal lining and associated nuclei that will form the rhomboid fossa. This inductive process ensures the precise dorsoventral organization essential for the fossa's diamond-shaped configuration.[16][17]By the fifth week of gestation (Carnegie stage 14), the rhomboid fossa becomes apparent as part of the fourth ventricle's anlage, coinciding with the initial expansion of the rhombencephalic cavity. At this stage, the fossa's outline emerges in the ventral wall of the developing ventricle, marking the early delineation of its boundaries within the pons and medulla precursors.Genetic regulation of the rhomboid fossa's origins involves Hox transcription factor genes, which provide the combinatorial codes for rhombomere-specific identities in the rhombencephalon. Hox genes, such as Hoxa1, Hoxb1, and members of the Hoxb cluster, are expressed in nested domains across r1 to r8, with specific combinations (e.g., Hoxb1 restricted to r4) directing segmental patterning and the positioning of ventral midline elements that contribute to the fossa. These genes integrate upstream signals like retinoic acid to refine rhombomere boundaries and ensure aligned neuronal differentiation along the future rhomboid fossa.[18]
Formation and maturation
The formation of the rhomboid fossa begins during the early embryonic period as the hindbrain undergoes significant flexures that expand the floor of the developing fourth ventricle. During the fifth week of gestation, the pontine flexure emerges at the level of the future pons, causing the neural tube to bend dorsally and "gape open" along its line of fusion, which directly contributes to the diamond-shaped contour of the rhomboid fossa.[19][20] Concurrently, the medullary (or cervical) flexure at the junction of the medulla oblongata and spinal cord further widens the ventricular floor between weeks 6 and 8, establishing the foundational rhomboid morphology as the alar plates shift laterally relative to the basal plates.[19]By approximately 12 weeks of gestation, the caudal end of the fourth ventricle narrows at the obex, marking the transition to the central canal of the spinal cord and delineating the calamus scriptorius region of the rhomboid fossa.[21] This process refines the fossae's boundaries, separating the open portion of the fourth ventricle from the closed medullary segment and ensuring proper cerebrospinal fluid containment.[22]Postnatally, the rhomboid fossa exhibits minimal structural growth, as its dimensions are largely established in utero, but underlying neural maturation continues through myelination of brainstem tracts and nuclei. Myelination in the brainstem, including fibers adjacent to the fossae floor, progresses rapidly from late gestation and reaches completion by approximately 2 years of age, enhancing signal conduction efficiency without altering the gross anatomy.[23][24]Developmental anomalies, such as those seen in Chiari malformations, can disrupt this process by causing underdevelopment of the posterior cranial fossa, leading to altered rhomboid fossa shape due to cerebellar tonsillar herniation that compresses the fourth ventricle floor.[25]
Function
Role in CSF circulation
The rhomboid fossa, forming the floor of the fourth ventricle, plays a key role in directing cerebrospinal fluid (CSF) flow through the coordinated ciliary action of its ependymal lining. The ependyma, a specialized epithelial layer covering the fossa, features motile cilia that beat rhythmically to propel CSF entering from the cerebral aqueduct toward the lateral apertures, known as the foramina of Luschka.[1] This ciliary propulsion is essential for maintaining unidirectional flow within the narrow confines of the fourth ventricle, ensuring efficient circulation without stagnation.[26] The ependymal cilia, protruding from multiciliated cells, generate a metachronal wave that facilitates this transport, with beating frequencies typically ranging from 28 to 40 Hz in mammalian models.[27]Integration with the choroid plexus further enhances the rhomboid fossa's contribution to CSF dynamics, particularly in the lateral recesses. The choroid plexus, a vascularized structure within these recesses, contributes to total CSF production, which occurs at a rate of approximately 0.3–0.4 mL/min in adults, with the fourth ventricle accounting for about 10–20% of the total daily CSF volume.[28] This newly formed CSF mixes with incoming fluid from the aqueduct and is directed by ependymal cilia toward the foramina of Luschka, where the plexus itself protrudes to facilitate direct release into the subarachnoid space.[1] Such integration ensures a steady supply and distribution of CSF, supporting nutrient delivery and waste removal in the posterior fossa.The thin structure of the rhomboid fossa's floor also aids in pressure regulation by allowing transmission of subarachnoid space pressure changes to the ventricular interior. Composed of a delicate layer of neural tissue and ependyma, the fossa acts as a compliant barrier that equalizes hydrostatic pressures, preventing excessive ventricular dilation during fluctuations in intracranial pressure.[1] This mechanism is crucial for maintaining CSF homeostasis, as it permits subtle adjustments in response to pulsatile arterial flows or postural changes.At its inferior apex, the obex marks the narrowing of the fourth ventricle into the central canal of the spinal cord, directing the majority of CSF outward through the apertures while only a small amount enters the canal due to the anatomy and flow dynamics.[1] This configuration helps sustain forward momentum in CSF circulation, reducing the risk of accumulation in the lower ventricular extensions.[29]
Associated brainstem nuclei
The rhomboid fossa, forming the floor of the fourth ventricle, harbors several key brainstem nuclei associated with cranial nerve functions, embedded within its pontine and medullary portions.[13] These nuclei include both motor and sensory components that contribute to essential neural signaling pathways.[2]In the pontine region, motor nuclei are prominently featured. The abducens nucleus (cranial nerve VI), located in the dorsal aspect of the caudal pons near the midline, underlies the facial colliculus and controls lateral rectus muscle innervation for eye abduction.[13] Adjacent to it, the facial motor nucleus (cranial nerve VII) resides deeper in the pontine tegmentum, with its axons forming intra-axial loops that course dorsomedially over the abducens nucleus, elevating the overlying facial colliculus before exiting the brainstem.[13]Shifting to the medullary segment, additional motor nuclei are situated within specialized trigones of the rhomboid fossa floor. The hypoglossal nucleus (cranial nerve XII), positioned in the hypoglossal trigone medial to the vagal trigone, provides somatic motor innervation to the tongue muscles.[14] The dorsal motor nucleus of the vagus (cranial nerve X), occupying the medial third of the vagal trigone (also known as the ala cinerea), serves as the primary parasympathetic outflow for visceral organs in the thorax and abdomen.[14]Sensory nuclei are also integral to the rhomboid fossa's structure. The vestibular nuclei (cranial nerve VIII), comprising superior, medial, lateral, and inferior components, lie within the vestibular area (area acustica) lateral to the sulcus limitans, processing balance and spatial orientation inputs from the inner ear.[2] The nucleus of the solitary tract, receiving sensory afferents from cranial nerves IX and X, extends across the lateral two-thirds of the vagal trigone, handling visceral sensory information including taste and baroreception.[14]A notable special feature is the locus coeruleus, a noradrenergic nucleus appearing as a bluish-gray area above the superior fovea in the upper rhomboid fossa; it consists of pigmented neurons that modulate arousal, attention, and stress responses throughout the central nervous system.[1]
Clinical significance
Pathological conditions
The rhomboid fossa, forming the floor of the fourth ventricle, can be distorted in Dandy-Walker malformation, a congenital anomaly characterized by cystic dilation of the fourth ventricle due to failure of integration of the anterior membranous area in the plica choroidalis, leading to hypoplasia or agenesis of the cerebellar vermis and elevation of the tentorium cerebelli.[25] This enlargement results in a large posterior cyst representing the dilated fourth ventricle, which compresses and distorts the rhomboid fossa, often accompanied by hydrocephalus in up to 80% of cases.[28] Clinical manifestations include ataxia, developmental delay, and increased intracranial pressure, with the distortion potentially impairing cerebrospinal fluid circulation at the ventricular outlets.[25]Lesions involving the area postrema, a circumventricular organ located at the caudal end of the rhomboid fossa in the dorsal medulla, are implicated in various pathological states. In neuromyelitis optica spectrum disorders, often overlapping with multiple sclerosis, inflammatory plaques selectively target aquaporin-4 channels in the area postrema, causing tissue rarefaction, vascular thickening, and immune cell infiltration without significant neuronal loss.[30] These lesions, observed in approximately 40% of cases, manifest as area postrema syndrome with intractable nausea, vomiting, and hiccups, increasing the odds of these symptoms by 16-fold compared to unaffected patients.[30] Additionally, the area postrema serves as the chemoreceptor trigger zone for emesis, where chemotherapy agents stimulate dopamine and serotonin receptors, inducing nausea and vomiting through activation of the vomiting center in the medulla.[31]Tumors originating from the ependymal lining of the rhomboid fossa, such as ependymomas and medulloblastomas, pose significant risks due to their location and potential for obstructive hydrocephalus. Ependymomas, the third most common pediatric brain tumor, frequently arise in the fourth ventricle adjacent to the rhomboid fossa, exhibiting a nodular pattern with perivascular pseudorosettes and often infiltrating the floor, complicating surgical resection.[28] Medulloblastomas, the most common malignant pediatric brain tumor, can originate from embryonic cells in the inferior rhomboid fossa or external granular layer, with 45% of those arising from the brainstem showing infiltrative attachment to the caudal rhomboid fossa in surgical cases, leading to symptoms like headache, vomiting, and cerebellar dysfunction.[32] These tumors may disseminate via cerebrospinal fluid pathways, necessitating multidisciplinary management.[32]Inflammatory conditions like rhombencephalitis directly affect the rhomboid fossa by targeting embedded brainstem nuclei, resulting in cranial neuropathies. Listeria monocytogenes is the most common cause, leading to brainstem encephalitis with involvement of cranial nerve nuclei in the pons and medulla, commonly manifesting as ophthalmoplegia, facial weakness, and dysphagia.[33] The inflammation disrupts nuclear groups along the rhomboid fossa, such as the abducens and facial nuclei, causing a spectrum of deficits including ataxia and respiratory compromise.[34] Autoimmune variants may similarly inflame these structures, exacerbating neuropathies through immune-mediated damage.[33]Vascular pathologies, particularly infarcts in the posterior inferior cerebellar artery (PICA) territory, underlie Wallenberg syndrome (lateral medullary syndrome), involving the rhomboid fossa-related sensory nuclei in the medulla. The infarct disrupts the spinal trigeminal nucleus and tract, as well as the nucleus of the solitary tract—both embedded in the floor of the fourth ventricle—producing ipsilateral facial sensory loss and contralateral body hypoalgesia due to deafferentation of second-order neurons. Additional features include Horner syndrome, dysphagia, and vertigo from involvement of adjacent sympathetic fibers and vestibular nuclei, with the rhomboid fossa's superficial position rendering these nuclei vulnerable to ischemic insult. Prognosis varies, but sensory deficits often persist, highlighting the critical role of these fossa-associated structures in sensory integration.
Diagnostic and surgical considerations
Magnetic resonance imaging (MRI), particularly T2-weighted sequences, is essential for visualizing the rhomboid fossa, as it delineates the floor of the fourth ventricle with high contrast, revealing abnormalities such as hyperintensities or flattening associated with underlying pathologies.[35][36] T2-weighted MRI also aids in assessing lesions extending along the rhomboid fossa, facilitating preoperative planning without obstructing cerebrospinal fluid (CSF) flow visualization.[37] Computed tomography (CT) cisternography complements MRI by evaluating CSF dynamics around the fourth ventricle, identifying obstructions at the rhomboid fossa level through contrast-enhanced flow patterns.[21][38]Endoscopic techniques provide direct intraventricular visualization of the rhomboid fossa during procedures for hydrocephalus, such as third ventriculostomy or foramen of Magendie opening, allowing assessment of floor integrity and outlet patency in cases of obstruction.[39][40]Surgical access to the rhomboid fossa typically involves suboccipital craniotomy for posterior fossa tumors, enabling telovelar or midline approaches while prioritizing preservation of the fossa's neural elements to minimize brainstem disruption.[41][42] Intraoperative monitoring using cranial nerve evoked potentials, including corticobulbar motor evoked potentials, protects associated brainstem nuclei by detecting real-time functional changes during tumor resection near the fourth ventricle floor.[43][44]Rhomboid fossa involvement in tumors, such as ependymomas, serves as a prognostic factor, indicating elevated risk of persistent hydrocephalus due to impaired CSF pathways, with incomplete resection further exacerbating postoperative complications.[45][46]