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Scilloideae

Scilloideae is a of bulbous, perennial monocotyledonous plants within the family , encompassing approximately 60 genera and more than 1,000 of geophytes adapted to seasonally dry climates. Formerly recognized as the separate family Hyacinthaceae, it is now classified under according to the . The is divided into four tribes—Hyacintheae, Ornithogaleae, Oziroëeae, and Urgineeae—reflecting phylogenetic relationships established through molecular studies. These plants typically feature a basal of fleshy, often mucilaginous leaves, underground with contractile roots for anchorage, and racemose inflorescences bearing flowers with six similar tepals, six stamens, and a superior . Floral varies widely, from star-shaped to tubular forms, attracting pollinators such as and , with occurring via or vegetative bulb offsets. Scilloideae exhibits a predominantly distribution, with the greatest diversity in Mediterranean regions, , , and , though a few genera extend to . They thrive in temperate to subtropical habitats, including woodlands, meadows, and arid scrublands, often emerging in to exploit seasonal moisture. Notable genera include Hyacinthus (hyacinths), (grape hyacinths), (squills), and (star-of-Bethlehem), many of which are popular in for their vibrant blooms. Some , such as (squill), have medicinal applications for cardiac conditions due to bioactive cardiac glycosides, while others are toxic and require caution in cultivation. Taxonomic boundaries within the subfamily remain dynamic, with ongoing revisions based on phylogenomic data to resolve generic circumscriptions.

Description

General morphology

Plants in the Scilloideae subfamily are typically bulbous geophytes, characterized by underground storage organs that may be tunicated or tunicless, enabling dormancy during unfavorable seasons. These bulbs vary in size and structure across genera, with some, like those in Drimia, reaching substantial dimensions up to football-sized. Vegetatively, Scilloideae produce a basal of fleshy, mucilaginous leaves that are often linear to lanceolate in shape, with parallel venation and entire margins that can be smooth or undulate. emerge seasonally and provide storage for nutrients, contributing to the plant's geophytic habit. Inflorescences arise from leafless scapes and take the form of racemes, spikes, or capitate clusters, sometimes appearing umbellate in certain genera. The flowers are actinomorphic and hermaphroditic, featuring a of six similar, petal-like tepals arranged in a single whorl, which may be or basally connate. The androecium consists of six stamens with filaments that are or fused at the , and dorsifixed anthers that dehisce longitudinally. The gynoecium includes a superior composed of three fused carpels, forming a three-loculed structure with axile ; the style is simple or divided into three lobes. Fruits are loculicidal capsules that dehisce along three valves, containing black seeds that are often winged, angled, or flattened. Plant height varies widely, from as low as 5 in some species to over 1 m in genera like Drimia. Key diagnostic traits of Scilloideae within include the bulbous habit, the undifferentiated of six tepals, and the presence of steroidal in plant s, which contribute to their .

Reproductive structures

The reproductive structures of Scilloideae are characterized by a trilocular, superior that is typically syncarpous and contains numerous ovules per locule. The ovules are anatropous, bitegmic, and crassinucellate, featuring a chalazal hypostase—a specialized at the that aids in conduction and embryo sac development. These features are evident in genera such as , where the hypostase develops as a distinct parenchymatous region post-megasporogenesis, supporting the persistent antipodal cells in the embryo sac. production occurs in septal nectaries located within the walls, extending from the to the style; these glands secrete colorless to yellowish that accumulates in the locules and is released via slits at the , attracting pollinators in like Massonia. Fruit development follows , with capsules maturing over 3–6 months and dehiscing loculicidally along the septa to release . In genera like Austronea, fruits form within 2–3 months post-flowering, transitioning from green ovaries to dry, dehiscent capsules that split longitudinally, though timelines vary slightly by and . exhibit diverse morphologies but are often flattened and winged in tribes such as Ornithogaleae, facilitating primary dispersal over short distances. Elaiosomes on in genera like promote secondary by ants, enhancing dispersal beyond reliance. Asexual reproduction is prevalent through vegetative means, including bulb offsets and bulbils that form adventitiously around the parent , allowing clonal in genera such as , where offsets detach and establish independently. Stolons occur in certain species, producing daughter bulbs at their tips for horizontal spread. This mode supplements , particularly in disturbed habitats. Cytologically, Scilloideae display a base chromosome number ranging from x=5 to x=8 across tribes, with widespread and contributing to morphological stasis despite genomic variation; for instance, diploids (2n=10–16) occur alongside higher polyploids in genera like Bellevalia and Lachenalia. structure supports reproduction, with pedicels bearing variable bracts by —e.g., ebracteate racemes in Hyacintheae reduce visibility to herbivores while exposing flowers for .

Taxonomy

Phylogenetic position

Scilloideae is recognized as a subfamily within the order and the family , comprising one of three main subfamilies alongside and . This placement reflects the expanded circumscription of in modern classifications, which incorporates diverse monocot lineages previously treated in separate families. In the phylogenetic framework of APG IV, Scilloideae occupies a basal position as the to the core clades that include and , supported by analyses of multiple molecular datasets. Molecular markers such as plastid genes rbcL and matK, combined with nuclear ribosomal ITS sequences, have revealed that the divergence of Scilloideae from these sister subfamilies aligns with crown age estimates for . Defining synapomorphies for Scilloideae include septal ies—a feature shared across but functionally adapted in this subfamily for nectar production—and a predominantly bulbous geophytic that facilitates and storage in seasonal environments. These traits distinguish Scilloideae while linking it to broader patterns. Recent phylogenomic studies, such as those employing high-throughput sequencing of hundreds of low-copy nuclear loci post-2020, have robustly confirmed the of Scilloideae, with bootstrap support values consistently exceeding 95% across analyses. The subfamily Scilloideae fully encompasses what was formerly classified as the separate family Hyacinthaceae, an integration driven by molecular evidence that demonstrated their close relationship within , eliminating the need for distinct familial status under APG IV.

Classification history

The classification of Scilloideae traces back to , who in 1753 established the genus within the family in his , designating as the for a group of bulbous monocots characterized by their six tepals and scapose inflorescences. In the 19th century, botanists began separating these taxa from the expansive Liliaceae; John Gilbert Baker erected the family Hyacinthaceae in 1871 to encompass genera like Scilla and Hyacinthus, emphasizing their distinct floral and bulb structures over the broader lily assemblage. Adolf Engler formalized Scilloideae as a subfamily of Liliaceae sensu lato in his 1892 Syllabus der Pflanzenfamilien, grouping it with other petaloid monocots based on morphological affinities such as synapomorphic stamen filaments. John Hutchinson reinforced this treatment in 1934, recognizing Scilloideae within Liliaceae in his Families of Flowering Plants, highlighting its position among advanced lilialean groups through comparative anatomy. Following , Rolf M. T. Dahlgren elevated Scilloideae to the family Hyacinthaceae in 1985, defining it with 15 tribes based on extensive morphological and anatomical surveys, marking a significant shift toward recognizing its distinct evolutionary lineage. Debates persisted over generic boundaries, particularly regarding Urginea, where J. P. Jessop in 1970 argued for its separation from broader alliances in southern African taxa, citing differences in seed morphology and architecture to justify segregate genera like . By the late , pre-molecular classifications estimated Hyacinthaceae at around 50 genera and 800 species, often involving frequent lumping and splitting, with functioning as a heterogeneous catch-all for diverse African and Eurasian elements. This era's taxonomy laid the groundwork for later molecular refinements under the systems.

Modern taxonomy

The modern taxonomy of Scilloideae is grounded in the (APG) classifications, which integrate molecular data to recognize it as a monophyletic within . APG III (2009) first formally delimited Scilloideae, encompassing the former Hyacinthaceae s.s., Oziroeaceae, Ornithogaloideae, and Urgineoideae as a cohesive group based on phylogenetic analyses of and nuclear markers. APG IV (2016) reaffirmed this circumscription without major alterations, emphasizing its distinct position among asparagoid lilies through shared synapomorphies like bulbous habit and structure. The subfamily is currently divided into four tribes: Hyacintheae (the largest, comprising approximately 20-25 genera), Ornithogaleae, Urgineeae, and the monotypic Oziroëeae. This tribal framework, established in post-2000 molecular studies, reflects in floral and vegetative traits across distributions. Hyacintheae dominates in generic diversity, particularly in and , while the other tribes show more restricted patterns. Within Hyacintheae, subtribal divisions include Hyacinthinae and Muscarinae, as outlined in Manning et al. (2004), with updates in subsequent works incorporating new phylogenetic data up to 2022. These subtribes distinguish groups based on inflorescence architecture, seed morphology, and chromosome features, aiding resolution of polyphyletic assemblages. Scilloideae encompasses approximately 60-70 genera and over 1000 species, according to estimates from as of 2025. Key revisions from 2011 to 2023, led by Martínez-Azorín et al., have split the polyphyletic s.l. into more than 15 genera (e.g., Albarrania, Biarum, and Pseudoruth) within Ornithogaleae, using combined morphological and molecular evidence to reflect natural clades. Recent additions include new species and combinations in Drimia (Urgineeae), such as transfers from Geschollia described between 2021 and 2025, and further 2025 descriptions like Drimia courtallensis from , updating southern African and Asian diversity.

Tribes

The Scilloideae subfamily is classified into four tribes—Oziroëeae, Ornithogaleae, Urgineeae, and Hyacintheae—based on phylogenetic analyses of molecular data and morphological characters such as structure and floral traits. This tribal framework, established by Chase et al. in 2009, reflects the evolutionary diversification within the subfamily, with ongoing refinements driven by and studies of evolution. Oziroëeae comprises a single , Oziroe, with three endemic to southern , marking a notable biogeographic disjunction from other tribes in the predominantly subfamily. These scapose geophytes produce blue flowers on leafless stems, with diagnostic features including stamens adnate to the tepals, rounded seeds, and an as long as the . Ornithogaleae, the second-largest , encompasses approximately 15 genera and over 300 species, primarily centered in but extending to and . Members exhibit white or yellow star-shaped flowers adapted to diverse habitats, with key genera such as , which has undergone taxonomic splits including segregates like Elsia based on molecular and morphological distinctions. Urgineeae includes around 10 genera and about 200 , occurring in arid regions of , , and southwestern . These robust plants feature tall scapes arising from red-brown bulbs, often with medicinal properties; prominent genera include Drimia and Urginea, noted for their bulb chemistry and ecological adaptations to dry environments. Recent phylogenetic studies have refined tribal boundaries through extensive sampling of and DNA, revealing inflorescence variation (e.g., simple racemes to branched structures) and supporting narrower generic circumscriptions amid ongoing taxonomic debate. Hyacintheae, the largest and most diverse , contains approximately 20-25 genera and over 500 , mainly distributed across the and , with extensions into . Characterized by varied inflorescences ranging from loose racemes to dense spikes—as seen in Hyacinthus—the tribe includes subtribes such as Hyacinthinae and Massoniinae, encompassing genera like Hyacinthus, , and with blue to purple star-like or tubular flowers.

Genera

The subfamily Scilloideae includes approximately 60-70 genera, distributed primarily across , , and parts of the , with a total exceeding 1,000 . These genera are characterized by geophytic habits, typically featuring underground bulbs or rhizomes adapted to seasonal climates. Taxonomic revisions continue to refine generic boundaries based on molecular and morphological data, resulting in both splits and mergers. Key genera within Scilloideae exhibit diverse bulb structures, from tunicated to tunicless forms, and vary in distribution and ecological roles. For instance, Drimia (approximately 100 ) is a large genus of robust, often with solid bulbs, predominantly found in arid regions of and the . Hyacinthus (about 30 ) comprises ornamental bulbous perennials with fragrant, clustered flowers, native to the Mediterranean and western , featuring tunicated bulbs covered in dry layers. (around 60 ) includes small-bulbed geophytes with grape-like inflorescences, widespread in temperate . Scilla s.s. (roughly 80 ), after recent recircumscriptions, consists of spring-flowering with fibrous-coated bulbs, mainly in and . Bellevalia (approximately 50 ) features elongated bulbs and is centered in the . Other notable genera include (6 ), a North American group with starchy bulbs sometimes debated for its precise phylogenetic placement within Scilloideae due to early classifications in separate families, and the endemic South Daubenya (4 ), known for its sessile, ground-hugging flowers and tunicless bulbs.
GenusApproximate Species CountKey Traits and Distribution
Albuca60Ornithogaleae; spiral leaves,
Barnardia2Hyacintheae; coastal
Bowiea1Urgineeae; climbing vine-like,
Camassia6Hyacintheae; edible bulbs,
Chionodoxa6Hyacintheae; snowmelt flowers,
Daubenya4Massonieae; endemic to Cape region,
Drimia100Urgineeae; robust bulbs, Africa/Arabia
Hyacinthus30Hyacintheae; fragrant spikes, Mediterranean
Ledebouria60Massonieae; spotted leaves,
Muscari60Hyacintheae; grape hyacinths,
Ornithogalum150Ornithogaleae; starry flowers, widespread
Scilla80Hyacintheae; restricted to /
Recent taxonomic changes post-2020 have included the description of new genera, such as Occultia (2 species) in 2022, segregated from based on phylogenetic analyses of East African material from and , featuring distinct and traits. Mergers have also occurred, with incorporating additional species previously under or related segregates during revisions of the Massonieae subtribe, reflecting closer molecular affinities. In the Ornithogaleae tribe, ongoing studies have prompted splits, including potential new genera from South African lineages identified in 2023 phylogenetic work. Additionally, Drimia has seen expansions, with at least five new species described in 2024 from southern African floras and further additions in 2025, such as Drimia courtallensis from , emphasizing the dynamic nature of Scilloideae classification.

Distribution and ecology

Geographic distribution

The subfamily Scilloideae () is primarily distributed across the , with the majority of its approximately 1,000 species occurring in and a substantial number in , reflecting a bimodal pattern of diversity centered in seasonal and Mediterranean climates. A smaller proportion extends to the , including genera like in and Oziroë in , likely resulting from long-distance dispersal events. Centers of diversity are concentrated in , particularly the , which harbors high levels of and supports numerous genera such as Lachenalia (over 140 , nearly all endemic to ) and Massonia (15 in the core Cape area, 13 endemic). The represents another major hotspot, exhibiting high diversity with disjunct distributions linking Eurasian and African lineages, while serves as a secondary center for genera like Dipcadi (about 40 across , , and the ). Endemism is particularly pronounced in southern Africa, where many species in tribes like Urgineeae are confined, underscoring the area's role as a biogeographic hub (e.g., genera Drimia and Austronea, with 21 species restricted to the region). Disjunct patterns are evident in shared Eurasian-African clades, such as those in Scilla and Muscari, which bridge continents via ancient dispersal routes. Introduced ranges have expanded beyond native areas through human activity, including Scilla peruviana naturalized in California and Muscari species in Australia. Biogeographically, clades trace origins to Gondwanan ancestors in sub-Saharan regions, while Eurasian lineages align with Laurasian histories, with evidence and molecular dating indicating diversification around 40 million years ago during the Eocene-Oligocene transition. Recent studies highlight ongoing shifts due to , such as modeled northward range expansions for Iberian Scilla species under future warming scenarios (2081–2100), with increased variability in non-overlapped distribution areas across the western Mediterranean.

Habitat preferences

Scilloideae species predominantly inhabit regions with Mediterranean-type climates, featuring mild, wet winters and hot, dry summers, which align with their growth cycles as spring-flowering geophytes. This preference is evident in their native ranges across the , where species like Scilla hyacinthoides emerge during the rainy season to complete reproduction before summer drought sets in. Well-drained sandy or loamy soils are essential, preventing waterlogging during wet periods while supporting root development in nutrient-variable substrates; for instance, Drimia altissima thrives on sandy substrates in southern African grasslands. As bulbous geophytes, Scilloideae exhibit key adaptations for surviving arid conditions, including tunicated bulbs that store water and nutrients, enabling through dry seasons. In fire-prone ecosystems like the of , species such as those in Lachenalia persist via resprouting from underground organs post-fire, enhancing resilience in nutrient-poor, oligotrophic environments. Their altitudinal distribution is broad, from coastal dunes to montane zones exceeding 3,000 m; alpine taxa like Puschkinia scilloides occupy subalpine meadows and rocky slopes in the , where cold winters and short growing seasons favor compact growth forms. Soil preferences lean toward neutral to alkaline pH levels, typically 6.5–7.15, as observed in habitats of various species, which tolerate substrates common in Mediterranean and regions. Mycorrhizal associations, particularly arbuscular types, facilitate nutrient uptake—especially —in these often impoverished soils, boosting colonization intensity and supporting growth in low-fertility conditions. Tribal variations reflect ecological specialization: Urgineeae members, such as Drimia and Spirophyllos, favor semi-arid and desert fringes with sparse vegetation, while Hyacintheae taxa like Hyacinthus and occur in open woodlands and meadow edges with moderate moisture. Habitat loss from poses a significant threat, fragmenting populations in fertile lowlands and coastal plains across their range. Recent studies from 2022–2025 highlight vulnerability to driven by , with modeling for Mediterranean Scilloideae projecting up to 20% range contraction by 2050 due to shifting patterns and increased intensity (as modeled in 2014).

Pollination and reproduction

Members of the Scilloideae subfamily exhibit diverse pollination strategies adapted to their habitats, primarily involving insects such as bees, butterflies, moths, and occasionally birds in African lineages. In genera like Lachenalia, bees serve as the main pollinators for the majority of species, while bird pollination by sunbirds (e.g., Anthobaphes violacea and Cinnyris chalybeus) occurs in 14 species, representing multiple independent evolutionary shifts favored in fynbos habitats with low insect abundance. Similarly, in Hyacintheae genera such as Muscari, bees forage for nectar and pollen, contributing to effective cross-pollination. For nocturnal species like Dipcadi saxorum in the same tribe, settling and hovering moths (e.g., Heliothis peltigera and Macroglossum stellatarum) are primary pollinators, attracted by foul-acrid odors composed of aldehydes and esters. Floral traits in Scilloideae align with pollinator syndromes, enhancing attraction and reward delivery. Blue and violet flowers, common in genera like Scilla and Hyacinthus, target diurnal insects such as bees and butterflies, while off-white or pale blooms in species like Dipcadi saxorum suit generalist or nocturnal moths. Nectar, produced via septal nectaries in the ovary, serves as the primary reward, secreted in small volumes (e.g., ~1 µl per flower in D. saxorum) shortly after dusk to align with pollinator activity. These nectaries, a characteristic feature of Asparagales including Scilloideae, facilitate precise nectar placement within the flower structure. Breeding systems in Scilloideae predominantly favor to promote , though self-compatibility has evolved repeatedly as a reproductive safeguard in pollinator-scarce environments. In Lachenalia, approximately 48% of species are self-compatible, often co-occurring with bird pollination or non-spring flowering , while autofertility—enabling seed production without pollinators—appears in 6 species across 5 origins. In and related genera, is common, enforced by mechanisms like protandry, but some populations exhibit partial self-compatibility to ensure under variable conditions. For instance, D. saxorum is fully , with 0% fruit set from . Seed production and dispersal in natural Scilloideae populations reflect their reliance on biotic interactions. Fruit set rates vary by species and habitat; in D. saxorum, natural open-pollination yields 24–27% fruit set, dependent on cross-pollination success. Seeds are typically dispersed by ants attracted to elaiosomes—lipid-rich appendages that serve as food rewards—resulting in short-distance transport of several meters from parent plants, enhancing establishment in suitable microsites. In disturbed habitats, asexual propagation via bulb offsets or bulbils supports persistence and spread, as seen in invasive populations of Muscari where vegetative reproduction via bulb division predominates. Recent research highlights as a growing threat to Scilloideae in Mediterranean regions, where warming and alteration reduce abundance and disrupt phenological synchrony. Studies from 2023 indicate that drivers, including drought and temperature shifts, alter plant- networks, leading to decreased visitation rates and potential reproductive assurance challenges for species. In southern African lineages like Lachenalia, habitats—already -limited—may see accelerated shifts toward self-compatibility under projected climate scenarios.

Human uses

Ornamental cultivation

Scilloideae species, particularly genera such as , , and , are widely cultivated for their vibrant spring blooms in gardens, borders, and indoor displays. is favored for forcing indoors to produce fragrant flowers during winter holidays, while excels in naturalizing lawns and woodland edges due to its ability to spread via offsets and tolerate mowing after foliage dies back. is commonly planted in spring borders for its early blue flowers that emerge reliably in temperate climates. Propagation primarily occurs through bulb division in autumn, where offsets are separated from mature bulbs and replanted immediately to establish new clumps. For species preservation or larger-scale production, seeds can be sown on the surface of moist compost, with germination typically occurring within several weeks under controlled conditions around 15°C. Bulb division is preferred for hybrids to maintain desirable traits, as seed-grown plants may vary phenotypically. These plants thrive in USDA hardiness zones 4-8, requiring full sun to partial shade and well-drained, organically rich soil to prevent waterlogging during . Muscari and Scilla perform best in sandy or loamy soils with a to slightly alkaline , mirroring their native Mediterranean habitats. For Hyacinthus, forcing techniques involve pre-chilling bulbs at 4-9°C for 10-12 weeks to simulate winter , followed by transfer to a bright, cool indoor location (around 15-18°C) for blooming 3-4 weeks later. Common pests include bulb mites and slugs, while diseases such as Fusarium-induced bulb rot and fungal issues like botrytis blight can affect overcrowded or poorly drained plantings. Organic controls, including for mites and improved air circulation to deter fungi, are recommended to minimize chemical use in ornamental settings. Commercial production of Hyacinthus bulbs is centered in the , where cultivation spans thousands of hectares alongside other bulbs, contributing to exports valued at over 13 million euros in early 2022. Over 2,000 Hyacinthus cultivars have been developed historically, though fewer than 50 remain in widespread commercial use for their fragrance and color diversity. Recent trends emphasize sustainable sourcing, with South African producers adapting imported bulbs to local conditions for resilient, eco-friendly ornamental supply chains.

Medicinal applications

Drimia maritima, commonly known as squill, has been a cornerstone in medicinal applications within the Scilloideae subfamily due to its cardiac glycosides, particularly proscillaridin A, which exhibit cardiotonic effects similar to digitalis and have historically served as an alternative treatment for heart failure and dropsy. In ancient Greek medicine, Scilla species were employed as remedies for dropsy, leveraging their diuretic properties to alleviate fluid retention associated with cardiac conditions. Traditional uses extend to African folk medicine, where Urginea species, now classified under Drimia, are utilized for respiratory issues such as bronchitis, asthma, and cough, often prepared as expectorants to stimulate bronchial secretions. The primary active compounds responsible for these effects are bufadienolides, including scillaren A, first isolated and structurally elucidated from squill bulbs in the early , with key pharmacological studies advancing in the mid-1900s. These glycosides, such as proscillaridin A and scillaren A, inhibit Na+/K+-ATPase to enhance cardiac contractility, though their narrow limits widespread use. Typical medicinal dosages involve 0.1–0.5 g of standardized bulb powder daily, administered under medical supervision to avoid . Modern applications remain constrained by the compounds' toxicity, but recent research highlights potential in oncology, with Drimia extracts demonstrating cytotoxic and apoptotic effects against cancer cell lines, including breast and colon cancers, through mechanisms like reactive oxygen species generation and cell cycle arrest. Preclinical studies from 2023–2024 have explored these extracts for anti-proliferative activity, suggesting possible adjunctive roles in cancer therapy, though human clinical trials are limited and emphasize the need for purified, low-dose formulations. Preparations commonly include tinctures (0.6–2 mL doses) and syrups like squill , traditionally combined with honey or vinegar for respiratory relief, as seen in Persian medicine for management. Regulatory guidelines, such as those from the Commission's ESCOP monographs, classify squill as a prescription herb due to cardiac risks, contraindicating its use in , , or renal impairment, and advising against concurrent .

Culinary uses

Certain species within the Scilloideae subfamily have been utilized in traditional cuisines, primarily for their bulbs, which are harvested from wild populations in Mediterranean and North American regions. , known as tassel hyacinth, provides edible bulbs that are a staple in southern , particularly in Puglia, where they are prepared as "lampascioni." These bulbs possess a bitter that is mitigated through , making them a valued component of local dishes. Preparation of L. comosa bulbs typically involves them multiple times to remove acridity, followed by in and for preservation and consumption as an appetizer or . This method has historical roots in Mediterranean diets, with records indicating use by ancient Romans, who consumed the bulbs dressed with and as noted by . Nutritionally, the bulbs are low in calories, approximately 50 kcal per 100 g, and rich in fructans, which act as prebiotics supporting gut health. Other Scilloideae species contribute to indigenous and regional foods. Young leaves of some species, like M. neglectum, are occasionally incorporated into salads for their mild pungency in Mediterranean contexts, including Turkish preparations, though caution is advised due to potential in other plant parts and conflicting reports on —consumption should be verified with experts or avoided. Culinary use is restricted to non-toxic species, as most Scilloideae contain cardiac glycosides that render them inedible or poisonous; fewer than 5% of the over 1,000 in the subfamily are safely consumed. Sustainable harvesting of wild populations has gained attention, with 2025 IUCN guidelines emphasizing limits on extraction volumes and rotational harvesting to prevent depletion of threatened bulbous .

Toxicity

Toxic compounds

The primary toxic compounds in Scilloideae are bufadienolides, a class of cardiac glycosides characterized by a C-24 backbone with a six-membered α-pyrone ring at the C-17β position. These metabolites, including scillarenin, scilliroside, scillaridin A, and bufalin, are predominantly accumulated in the bulbs and leaves of genera such as Drimia (formerly Urginea) and . Concentrations typically range from 0.2% to 0.5% of dry weight in Drimia species, though levels can vary by up to twofold depending on environmental factors and plant variety. In addition to bufadienolides, Scilloideae plants produce steroidal , such as scillasaponins A, B, and C, which are triterpenoid or spirostanol glycosides capable of inducing by disrupting cell membranes. These are more abundant in seeds and bulbs, with spirostanol variants like those in and contributing to the overall profile. Unlike bufadienolides, saponin concentrations are not as precisely quantified but are noted to be significant in inedible species across the subfamily. Bufadienolides in Scilloideae are biosynthesized via the pathway, starting from precursors like or , with key enzymatic steps involving oxidases to form the characteristic ring. This pathway is most active in the Urgineeae tribe, where Drimia species exhibit higher bufadienolide yields compared to other genera. Detection of these compounds commonly employs (HPLC) coupled with UV or , revealing higher concentrations in bulbs (up to 0.5% dry weight) than in leaves or flowers (0.1-0.3%). These toxic metabolites serve an evolutionary role as chemical defenses against herbivores, deterring feeding through their potent cardiotoxic and hemolytic effects. Recent analyses, including a 2023 metabolomic study on Drimia maritima, have identified over 50 bufadienolide analogs across Scilloideae, highlighting structural diversity such as variations in glycosylation and hydroxylation patterns that enhance bioactivity.

Effects on humans and animals

Ingestion of Scilloideae , particularly their bulbs, poses significant risks to humans due to the presence of cardiac glycoside-like compounds such as bufadienolides, which can disrupt cardiac function. Common symptoms include severe , , , , cramping, and , with potential progression to cardiac arrhythmias in more severe cases. These effects mimic and typically onset within hours of consumption. Accidental ingestion, especially by children mistaking bulbs for edible onions or , can lead to life-threatening outcomes; while exact lethal doses vary by species, small quantities (on the order of a few grams of bulb material) have caused fatalities in folk medicine uses of red squill (). Vulnerable populations include foragers who misidentify wild Scilloideae species as safe edibles, leading to gastrointestinal distress and cardiovascular complications. In animals, Scilloideae toxicity is particularly pronounced in livestock, with species in causing substantial losses among sheep and goats through acute and cardiac effects. Poisoning often manifests as sudden death syndrome, characterized by rapid onset of , , tremors, and ventricular arrhythmias, sometimes resulting in krimpsiekte-like chronic cardiac glycoside intoxication. In rangelands, grazing on these plants during dry seasons heightens risks for small ruminants, with historical outbreaks reporting thousands of deaths. Pets, such as , may experience similar gastrointestinal and cardiac symptoms from bulb ingestion, as seen in cases involving or related genera, though birds exhibit lower sensitivity due to efficient emetic responses that prevent lethal absorption. Treatment for bufadienolide poisoning in both humans and animals focuses on and supportive care; administration of activated charcoal within hours of ingestion binds toxins, while fragments can reverse severe cardiac effects by neutralizing glycosides. Recent veterinary reports highlight the efficacy of this approach in pet cases, such as those from accidental consumption around 2022. Management strategies include garden barriers to deter pets and , alongside awareness campaigns for rangeland herders; veterinary guidelines emphasize early monitoring of and imbalances in suspected exposures.

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