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Total synthesis

Total synthesis is the complete chemical construction of a complex organic molecule, typically a natural product, from simpler, commercially available starting materials in a laboratory setting.^[1] This process involves designing and executing a sequence of chemical reactions to assemble the target molecule's carbon skeleton, functional groups, and stereochemistry, often requiring innovative strategies to overcome challenges in efficiency, selectivity, and yield.^[2] It represents a pinnacle of synthetic organic chemistry, where chemists replicate or modify nature's intricate architectures to verify structures, probe biological mechanisms, or generate analogs for therapeutic use.^[3] The field traces its origins to Friedrich Wöhler's groundbreaking synthesis of urea in 1828, which demonstrated that organic compounds could be created from inorganic precursors and shattered the vitalist theory.^[1] Early milestones included Emil Fischer's synthesis of glucose in 1890 and Robert Robinson's biomimetic construction of tropinone in 1917, marking the shift toward more complex targets.^[1] The mid-20th century ushered in the "Woodward Era," highlighted by R.B. Woodward's total synthesis of quinine in 1944—a critical antimalarial drug—and strychnine in 1954, which showcased unprecedented control over polycyclic alkaloid frameworks.^[4] Subsequent eras, led by E.J. Corey (e.g., prostaglandins in 1969) and K.C. Nicolaou (e.g., taxol in 1994), expanded the scope to even larger molecules like vancomycin (achieved by multiple groups including Evans, Nicolaou, and Boger in 1998–1999) and everninomicin (1999), incorporating advanced techniques such as retrosynthetic analysis and convergent assembly.^[1]^[5] Beyond academic achievement, total synthesis serves as a proving ground for new reaction methodologies and reagents, accelerating innovations in organic chemistry that underpin pharmaceuticals, materials science, and chemical biology.^[6] It confirms ambiguous natural product structures derived from isolation, enables scalable production when natural sources are scarce, and facilitates the creation of modified derivatives to optimize biological activity—exemplified by taxol analogs for cancer therapy.^[1] Despite challenges like lengthy step counts and low overall yields, ongoing advances in catalysis, automation, and computational design continue to make total synthesis more accessible and impactful.^[3]

Fundamentals

Definition and Scope

Total synthesis refers to the complete chemical construction of a complex organic molecule from simple, commercially available starting materials through a planned sequence of chemical reactions.^[7] This process typically targets intricate structures that are challenging to isolate or modify from natural sources, emphasizing the de novo assembly of the molecular framework.^[8] The scope of total synthesis primarily encompasses organic molecules derived from natural sources, such as terpenoids (including steroids), alkaloids (e.g., morphine), polyketides, peptides, and macrolides, which exhibit structural diversity originating from plants, microbes, and marine organisms.^[7] These targets are pursued to replicate the full architecture, including correct stereochemistry at multiple chiral centers, precise functional group transformations, and optimization of overall synthetic yield to ensure practicality.^[1] While the field occasionally extends to non-natural targets like pharmaceuticals or advanced materials, it explicitly excludes partial syntheses, which modify pre-existing complex intermediates, and semi-syntheses, which rely on natural product scaffolds for derivatization.^[8] Central to total synthesis is its role in validating proposed molecular structures through unambiguous construction and facilitating structure-activity relationship (SAR) studies by enabling the preparation of analogs for biological evaluation.^[7] Retrosynthetic analysis serves as a foundational planning tool in this domain, though detailed strategies are explored separately.^[1]

Aims and Objectives

Total synthesis pursues a variety of aims across academic, industrial, and scientific domains, each driven by the challenge of constructing complex molecules from simple precursors. In academia, it exemplifies synthetic ingenuity by enabling chemists to devise creative routes that showcase innovative problem-solving and aesthetic elegance in molecular assembly. These endeavors often prioritize brevity and efficiency, as seen in pursuits of streamlined pathways that minimize steps while maximizing strategic bond formations. Beyond demonstration, total synthesis fosters the invention of new methodologies, pushing the frontiers of reaction design and selectivity to tackle ever-more intricate targets. It also serves as a vital training ground, equipping graduate students and researchers with expertise in planning, execution, and optimization of multi-step sequences. Industrially, total synthesis addresses practical needs by enabling the large-scale production of natural products that are scarce or costly to extract from biological sources, thereby supplying materials for therapeutic development and clinical trials. For instance, it facilitates the creation of pharmaceutical agents like paclitaxel analogs, which have advanced cancer treatments. A key objective is the synthesis of structural analogs to explore structure-activity relationships, optimizing potency, bioavailability, or safety profiles for drug candidates. Ultimately, these efforts aim to establish scalable, cost-effective processes that align with manufacturing demands, bridging laboratory innovation with commercial viability. From a scientific perspective, total synthesis validates natural product structures, offering unambiguous proof when isolation yields insufficient material for advanced spectroscopy. It illuminates biosynthetic pathways by replicating or altering proposed mechanisms, revealing enzymatic efficiencies and evolutionary adaptations in nature. In chemical biology, synthesized compounds act as probes or tools, enabling studies of protein interactions, signaling cascades, and disease mechanisms that would otherwise be inaccessible. A central debate in the field revolves around the balance between elaborate, prestige-driven syntheses that emphasize molecular complexity and more pragmatic approaches focused on applicability and scalability. This tension is highlighted in concepts like the "ideal synthesis," which advocates for concise, waste-minimizing routes using only constructive reactions to achieve both intellectual rigor and real-world utility.

Historical Development

Early Milestones

The foundational milestone in total synthesis occurred in 1828 when Friedrich Wöhler synthesized urea from inorganic precursors, specifically by heating ammonium cyanate to produce the organic compound urea, thereby challenging the prevailing theory of vitalism that posited organic molecules could only be produced by living organisms. This breakthrough, detailed in Wöhler's publication in Annalen der Physik und Chemie, marked the birth of organic synthesis by demonstrating that organic compounds could be constructed in the laboratory without biological intervention.^[9] In the late 19th and early 20th centuries, total synthesis expanded to natural products, with Emil Fischer achieving the first synthesis of glucose in 1890 through a multi-step process starting from glycerol, which confirmed the compound's structure and stereochemistry while highlighting early challenges in controlling chiral centers.^[10] This work laid groundwork for carbohydrate chemistry. Shortly thereafter, Gustaf Komppa reported the total synthesis of camphor in 1903 via a sequence involving condensation of diethyl oxalate with 3,3-dimethylpentanoic acid to form camphoric acid, followed by decarboxylation and reduction, representing one of the first commercial-scale syntheses of a complex terpenoid.^[11] In 1901, Jokichi Takamine isolated and purified adrenaline (epinephrine) from adrenal glands, enabling its use as a pharmaceutical and paving the way for subsequent synthetic efforts, though full total synthesis came later in 1904 by Friedrich Stolz.^[12] By the mid-20th century, total synthesis had evolved to tackle more intricate molecules, exemplified by Robert B. Woodward and William E. Doering's 1944 synthesis of quinine, a 17-step process from 7-hydroxyquinoline that yielded the antimalarial alkaloid and demonstrated the feasibility of assembling polycyclic structures with precise stereochemistry.^[13] Woodward further advanced the field with his 1954 total synthesis of strychnine, a 29-step linear sequence culminating in the formation of the alkaloid's heptacyclic framework through a key intramolecular aldol condensation, underscoring the power of strategic bond-forming reactions despite low overall yields.^[14] Concurrently, Vincent du Vigneaud achieved the first synthesis of a polypeptide hormone in 1953 by assembling oxytocin—a nine-amino-acid cyclic peptide with a disulfide bridge—using carbodiimide-mediated coupling, which not only confirmed its structure but also earned him the 1955 Nobel Prize in Chemistry for pioneering peptide synthesis techniques. These early efforts introduced core concepts in total synthesis, including the shift toward multi-step sequences that required careful planning to manage complexity and yield, as seen in the progression from Wöhler's single transformation to Woodward's elaborate routes.^[15] Stereocontrol emerged as a persistent challenge, with Fischer's use of chiral resolutions and Woodward's reliance on asymmetric inductions highlighting the need for methods to dictate molecular handedness in natural products.^[10] Additionally, the distinction between linear strategies—building sequentially as in strychnine—and convergent approaches—assembling fragments late-stage as hinted in quinine—began to influence synthetic design, optimizing efficiency for increasingly demanding targets.^[16]

Modern Advances

The formalization of retrosynthetic analysis in the late 20th century marked a pivotal advancement in total synthesis, enabling chemists to systematically deconstruct complex target molecules into simpler precursors through logical disconnection strategies. Elias James Corey developed this methodology, which introduced concepts like transform-directed retrosynthesis and the use of synthons to guide synthetic planning, revolutionizing the design of multi-step routes. His work culminated in the 1990 Nobel Prize in Chemistry, recognizing its profound impact on organic synthesis efficiency and creativity. Building on these foundations, the 1970s and 1990s saw remarkable achievements in the total synthesis of highly complex natural products, demonstrating the feasibility of constructing intricate molecular architectures. The collaborative effort by Robert B. Woodward and Albert Eschenmoser completed the first total synthesis of vitamin B12 in 1972, involving over 90 steps and showcasing innovative ring-forming strategies for its corrin macrocycle. Similarly, in 1994, K. C. Nicolaou and Robert A. Holton independently achieved the total synthesis of taxol (paclitaxel), a diterpenoid anticancer agent with a unique oxetane ring and taxane skeleton, through convergent assemblies exceeding 30 steps. That same year, Yoshito Kishi's group reported the total synthesis of palytoxin, a marine toxin with 115 stereocenters and over 70 contiguous carbons, highlighting advanced stereocontrol in polyketide-like chains. These milestones underscored the growing capability to tackle molecules of unprecedented complexity, often requiring hundreds of person-years of effort. The late 20th and early 21st centuries witnessed the rise of asymmetric catalysis, which dramatically enhanced the enantioselectivity of total syntheses. In 2001, the Nobel Prize in Chemistry was awarded to William S. Knowles, Ryoji Noyori, and K. Barry Sharpless for their pioneering work on chirally catalyzed hydrogenation and oxidation reactions, enabling the production of enantiopure compounds from prochiral substrates with high efficiency.^[17] These methods, such as Noyori's ruthenium-based transfer hydrogenation and Sharpless's epoxidation, became integral to natural product syntheses, reducing reliance on chiral auxiliaries and improving overall step economy.^[18] From 2000 to 2025, total synthesis evolved through the integration of organocatalysis, photoredox catalysis, and C-H activation, allowing milder conditions, greater selectivity, and shorter routes to complex targets. Organocatalysis, employing small organic molecules like proline derivatives or cinchona alkaloids, facilitated asymmetric transformations in syntheses such as those of polyketides and alkaloids, often at room temperature without metals.^[19] Photoredox catalysis, leveraging visible light and transition-metal complexes (e.g., Ru or Ir polypyridyls), enabled radical-mediated bond formations under ambient conditions, as seen in late-stage functionalizations of terpenoids and heterocycles. C-H activation complemented these by directly functionalizing inert C-H bonds, streamlining routes to alkaloids and macrolides via palladium- or rhodium-catalyzed processes. Notable applications include Dale L. Boger's 2020 next-generation total synthesis of vancomycin aglycon, a glycopeptide antibiotic, achieved in 17 steps using atropselective couplings and peripheral modifications for enhanced activity against resistant bacteria. Likewise, Larry E. Overman's earlier ingenol synthesis (adapted in subsequent works) incorporated tandem cyclizations, while recent advances integrated these catalytic modes for scalability. Concurrently, AI-assisted planning tools like IBM RXN, launched in 2018, have accelerated retrosynthetic design by predicting reaction outcomes with neural networks trained on vast datasets, aiding multi-step planning for pharmaceuticals.^[20] Flow chemistry has further boosted scalability, enabling continuous multistep processes for natural product analogs, as exemplified in automated syntheses of complex heterocycles from 2015 onward. For instance, in 2024, Phil S. Baran's group reported the total synthesis of (−)-cylindrocyclophane A, employing asymmetric C-H borylation to streamline the construction of its unique cyclophane framework.^[21] These innovations have collectively reduced synthetic steps, improved yields, and expanded access to bioactive molecules up to 2025.

Synthetic Strategies

Retrosynthetic Analysis

Retrosynthetic analysis is a systematic technique for planning organic syntheses by deconstructing a target molecule into simpler precursor structures through a series of hypothetical bond-breaking steps known as disconnections, which generate idealized fragments called synthons.^[22] This reverse-engineering approach, pioneered by E.J. Corey, transforms the synthetic problem into a search for feasible forward reactions without presupposing available starting materials.^[22] By working backward from the target, chemists identify potential synthetic routes that align with known reactivity patterns. The core principles of retrosynthetic analysis revolve around transform-based reasoning, where synthetic reactions are inverted to define retrosynthetic transforms, and functional group interconversions (FGI) that adjust molecular functionality to enable disconnections.^[22] Transforms are guided by the presence of retrons—structural subunits in the target that match the reverse of a synthetic reaction—and emphasize simplifying transformations to reduce molecular complexity efficiently.^[22] Key rules include avoiding early branch points in the retrosynthetic tree to prevent combinatorial explosion and prioritizing disconnections that lead to stable, commercially available precursors.^[23] In designing routes, retrosynthetic analysis distinguishes between linear synthesis, where precursors are assembled sequentially in a single chain, and convergent synthesis, which involves parallel construction of subfragments that are coupled late-stage to minimize steps and improve overall yield.^[22] Protecting group strategies are integral, temporarily masking reactive functionalities during disconnections to allow selective transformations, while stereochemical considerations ensure that chiral centers are either preserved through stereospecific synthons or introduced via asymmetric disconnections that align with the target's configuration.^[22] Basic disconnection types facilitate this planning; for instance, carbonyl umpolung reverses the natural electrophilicity of a carbonyl carbon to nucleophilic reactivity, enabling synthons like acyl anions via dithiane or cyanide equivalents, as conceptualized by D. Seebach.^[24] Aldol equivalents, such as enolate-aldolhyde disconnections, target β-hydroxy carbonyl patterns by inverting the aldol addition, allowing construction of C-C bonds in complex frameworks.^[23] Computational tools enhance these efforts: Corey's LHASA (Logic and Heuristics Applied to Synthetic Analysis) program, developed in the 1970s, automates retrosynthetic exploration using heuristic rules and a database of transforms to generate pathways.^[23] Modern tools, like AI-driven platforms such as SynRoute, employ machine learning on reaction databases to predict multi-step routes with high accuracy for drug-like molecules.^[25] As of 2025, advanced generative models like RSGPT, pre-trained on billions of datapoints, further improve retrosynthetic planning with state-of-the-art accuracy in template-free predictions.^[26]

Key Methodologies

Total synthesis relies on a suite of classical methodologies for constructing carbon-carbon bonds, which form the backbone of complex molecular architectures. The Wittig reaction, involving the nucleophilic attack of a phosphonium ylide on a carbonyl compound to form an alkene and triphenylphosphine oxide, proceeds via a betaine intermediate that collapses to the oxaphosphetane, influencing stereoselectivity based on ylide stabilization—non-stabilized ylides favor Z-alkenes, while stabilized ones yield E-alkenes predominantly.^[27] This method's utility in total synthesis stems from its ability to introduce double bonds with controlled geometry, often achieving >90% E-selectivity in semi-stabilized cases through lithium salt-free conditions.^[28] Complementing this, the aldol reaction unites an enolate donor with a carbonyl acceptor to forge β-hydroxy carbonyls, guided by the Zimmerman-Traxler transition state that dictates stereoselectivity: chair-like conformations lead to syn or anti products depending on enolate geometry (Z-enolates favor syn aldols with diastereoselectivities up to 20:1).^[29] In synthetic applications, Lewis acid-mediated variants enhance efficiency by accelerating enolate formation and improving facial selectivity in chiral environments.^[30] The Diels-Alder reaction, a [4+2] cycloaddition between a diene and dienophile, occurs concertedly through a suprafacial transition state, enforcing stereospecificity where endo approaches dominate due to secondary orbital interactions, yielding cyclohexenes with diastereoselectivities often exceeding 95:5. These classical tools address early-stage complexity building by providing convergent, stereocontrolled access to carbocycles and functionalized chains essential for polyketide and alkaloid frameworks.^[31] Modern techniques have revolutionized total synthesis through asymmetric catalysis, enabling enantioselective transformations that minimize racemization and step count. The Sharpless epoxidation exemplifies this, converting allylic alcohols to epoxy alcohols using tert-butyl hydroperoxide (t-BuOOH), titanium(IV) isopropoxide, and a chiral diethyl tartrate ligand; the mechanism involves directed oxygen delivery from the allylic hydroxyl, achieving >95% ee via a Ti-peroxo intermediate that favors one face of the alkene.^[32]

\text{allylic alcohol} + t\text{-BuOOH} \xrightarrow{\text{Ti(O$i$Pr)}_4, \text{DET}} \text{epoxy alcohol}

This kinetic resolution and asymmetric induction have been pivotal in synthesizing chiral building blocks for natural products like taxol precursors. Organocatalysis, such as the proline-catalyzed aldol, employs L-proline as a bifunctional catalyst to generate enamines from ketones, which add to aldehydes with enantio- and diastereoselectivities up to 99:1, proceeding through an enamine-imine tautomerism that mimics enzymatic active sites. Transition-metal catalysis further expands options, with the Suzuki-Miyaura cross-coupling linking organoboronic acids and halides via a Pd(0)/Pd(II) cycle: oxidative addition of the halide, transmetalation with the boronate, and reductive elimination form biaryls, often with >98% yields and stereoretention for vinyl substrates.

\text{Ar-B(OH)}_2 + \text{Ar'-X} \xrightarrow{\text{Pd catalyst, base}} \text{Ar-Ar'} + \text{HX} + \text{H}_2\text{O}

This reaction's tolerance for aqueous conditions and functional groups has streamlined late-stage aryl couplings in alkaloid total syntheses.^[33] Advanced approaches integrate biological and physical innovations to tackle selectivity in complex settings. Chemoenzymatic methods leverage enzymatic resolutions, such as lipase-catalyzed kinetic resolutions of racemic alcohols or esters, achieving up to 99% ee by selective acylation of one enantiomer, often combined with chemical steps for scalable chiral pool access in terpenoid syntheses.^[34] Photoredox catalysis employs visible-light-activated catalysts like Ru(bpy)₃²⁺ to drive C-H functionalizations, such as α-amino C-H arylation via single-electron transfer and radical addition, enabling mild, site-selective modifications with yields >80% and minimal overoxidation.^[35] Flow chemistry facilitates continuous processing by pumping reagents through microreactors, enhancing heat/mass transfer for exothermic reactions like organometallic additions, reducing residence times to seconds and improving overall yields by 20-50% in multi-step sequences through inline purification.^[36] These methodologies integrate within multi-step total syntheses to enhance selectivity and efficiency, often following retrosynthetic planning to align reaction sequences that cascade stereocontrol—e.g., an asymmetric catalysis step sets chirality propagated through subsequent C-C formations, minimizing protecting groups and achieving step economies of 30-50% via convergent assembly.^[37] Such orchestration addresses challenges like epimerization in long sequences by employing orthogonal catalysts that operate under mild conditions, ensuring high fidelity in constructing polyfunctional targets.^[38]

Notable Total Syntheses

Classic Examples

One of the earliest landmark achievements in total synthesis was the formal synthesis of quinine by Robert B. Woodward and William von E. Doering in 1944, reported in a series of publications culminating in 1945. This 17-step route began with 7-hydroxyisoquinoline and constructed the core cinchona alkaloid framework, culminating in the preparation of d-quinotoxine, an advanced intermediate known from prior work to convert to quinine in three additional steps via the unverified Rabe-Kindler degradation and cyclization sequence. Key transformations included the assembly of the quinuclidine ring system through a series of alkylations and reductions, with quinoline formation achieved via initial isoquinoline modification and subsequent ring adjustments to establish the characteristic 6-methoxyquinoline moiety. Stereocontrol was achieved non-selectively, yielding a racemic mixture at the key C-8 and C-9 centers, reflecting the era's limitations in asymmetric induction; the overall yield to d-quinotoxine was approximately 1%, underscoring the inefficiency but highlighting innovative use of protecting groups and functional group interconversions. This work demonstrated early strategic retrosynthesis for alkaloid scaffolds, relying on classical condensations and hydrogenations to build complexity from aromatic precursors.^[13] The total synthesis of strychnine by Woodward in 1954 stands as a pinnacle of mid-20th-century organic synthesis, comprising a 29-step sequence that assembled the intricate heptacyclic indole alkaloid from commercially available materials like 3,4-dimethoxyphenyl methyl ketone. Commencing with a Fischer indole synthesis to form 2-veratrylindole, the route employed biomimetic strategies inspired by proposed biosynthetic pathways, including the construction of the tryptamine-like subunit and elaboration of the quaternary C-7 center via enamine alkylation. Critical steps involved multiple cyclizations, such as the Dieckmann condensation to forge the E ring and a stereoselective intramolecular Michael addition to establish the complex cage-like structure, culminating in aromatization and deprotection to yield (±)-strychnine. Stereochemistry at the seven chiral centers was controlled through substrate-directed epimerizations and selective reductions, though the synthesis produced the racemate; the overall yield was less than 0.1%, with only milligrams obtained after laborious purifications. This synthesis exemplified convergent assembly of polycyclic systems, integrating radical and ionic processes to mimic nature's efficiency while pioneering tactics like the use of methoxycarbonium ions for ring closure.^[14]^[16] The total synthesis of vitamin B₁₂ (cyanocobalamin), completed in 1972 through a collaborative effort between Woodward's Harvard group and Albert Eschenmoser's team at ETH Zurich, represented an unprecedented feat, involving over 100 steps to construct the corrin macrocycle and incorporate the central cobalt ion. The Harvard approach focused on the eastern (A/B/C) fragment via biogenetic-type cyclizations, while the ETH route emphasized the western (A/D) portion using thioether contractions; convergence occurred at the corphin stage, where the two asymmetric subunits were linked via iminoester condensation and sulfide extrusion to form the direct A/D bond characteristic of corrins. Key features included the stereocontrolled assembly of the contracted tetrapyrrole with nine asymmetric centers, metal coordination achieved by inserting Co²⁺ into the corrin ligand followed by axial ligation with dimethylbenzimidazole, and resolution of stereoisomeric mixtures (e.g., separating cobyrinate from neocobyrinate) using chromatography. Yields were exceedingly low, with final product obtained in microgram quantities after ~95 steps to heptamethylbisnorcobyrinate alone, reflecting the scale of the challenge. This dual-team convergence illustrated scalable fragment coupling for megasyntheses and advanced understanding of corrin stereodynamics.^[39] These classic syntheses highlight the evolution of total synthesis in the mid-20th century, with route lengths escalating from 17 steps for quinine's formal pathway to 29 for strychnine and over 100 for vitamin B₁₂, accompanied by overall yields dropping to trace levels due to accumulating inefficiencies in classical transformations. Lessons in stereochemistry emphasized the need for epimerization-resistant designs and chromatographic resolutions, as seen in B₁₂'s handling of multiple epimers, while convergence strategies—evident in B₁₂'s fragment merger and strychnine's polycycle builds—foreshadowed modular approaches to manage complexity. Collectively, they established benchmarks for tackling natural product architectures, prioritizing strategic bond disconnections over yield optimization.^[16]^[39]

Recent Achievements

One of the landmark achievements in the 1990s was the total synthesis of paclitaxel (Taxol), an anticancer drug, independently reported by K. C. Nicolaou and Robert A. Holton in 1994. Nicolaou's route involved a 30-step sequence featuring a challenging macrocyclization to form the eight-membered B-ring and subsequent attachment of the complex side chain, achieving the natural product in low overall yield but demonstrating innovative use of radical cyclizations and olefin metathesis. Holton's convergent approach, also approximately 37 steps, emphasized asymmetric dihydroxylation and esterification strategies for side-chain incorporation, enabling scalable production that contributed to Taxol's commercial availability. These syntheses highlighted the feasibility of constructing densely functionalized polycyclic structures, paving the way for analog development in chemotherapy. In the same year, Yoshito Kishi's group completed the total synthesis of palytoxin, the largest non-polymeric natural product known at the time with 64 stereocenters and a molecular weight exceeding 2,600 Da. The 113-step synthesis from simple precursors relied on a hybrid strategy combining partial synthesis of a key fragment with full de novo construction of others, using stereocontrolled aldol reactions and the novel Nozaki-Hiyama-Kishi coupling for C-C bond formation across the massive carbon skeleton. This monumental effort not only confirmed palytoxin's structure but also underscored the limits of classical organic synthesis, influencing subsequent work on complex marine toxins through modular assembly techniques. The late 1990s and 2000s saw continued progress with the synthesis of brevetoxin A by Nicolaou in 1998, a polycyclic ether toxin featuring 10 fused rings and responsible for red tide neurotoxicity. The 66-step route employed sulfone anion chemistry for ring closures and selective protecting group manipulations to weave the 44-carbon chain with 30 oxygen atoms, yielding the target in multigram quantities suitable for biological studies. For vancomycin, a glycopeptide antibiotic critical against Gram-positive bacteria, David A. Evans reported a total synthesis of the aglycon in 1999 using atropselective macrocyclization via aryl chloride arylation, followed by glycosylation. Dale L. Boger advanced this in 2001 with a biomimetic approach incorporating oxidative phenolic coupling, and by 2017, his group synthesized pocket-modified analogs via single-atom replacements (e.g., amidine at residue 4) to enhance binding affinity against resistant strains, reducing steps to under 40 while improving yields to 0.5%. In 2013, the group of Troels J. Jorgensen achieved a concise total synthesis of ingenol, the core of ingenol mebutate (Picato), an FDA-approved treatment for actinic keratosis, using a 14-step route from (+)-3-carene with pinacol coupling and oxidative dearomatization to form the strained cyclopropane and inside-out trans-fused rings.^[40] From 2020 to 2025, syntheses have emphasized efficiency and innovation, such as Masahiro Hirama's updated routes to ciguatoxin CTX3C in ongoing refinements, building on his 2001 total synthesis with improved protective group strategies for the 3-nm-long ladder polyether, enabling preparation of congeners like CTX1B for ciguatera research.^[41] Analogs of discodermolide, a microtubule-stabilizing anticancer agent, saw renewed interest in 2022 with desymmetrization approaches to stereogenic centers, shortening routes to 25 steps and facilitating SAR studies on lactone modifications.^[42] Notably, a next-generation total synthesis of vancomycin aglycon by Dale L. Boger in 2020 was completed in 17 steps with 5% overall yield, exemplifying AI-assisted design trends in modular assembly for resistant analogs.^[43] Over this period, trends in total synthesis have shifted toward shorter routes under 20 steps, as seen in modular assemblies using catalysis, with overall yields often exceeding 5% through atom-economical reactions like cross-coupling.^[44] Higher yields stem from asymmetric methods and protecting-group-free strategies, while sustainability is prioritized via biocatalytic steps and renewable feedstocks, reducing waste in large-scale preparations of pharmaceuticals.^[45]

Challenges and Future Directions

Current Limitations

Despite significant advances, total synthesis continues to face efficiency challenges, with many routes requiring over 20 steps for complex natural products, resulting in overall yields often below 1% for intricate targets like grayanotoxin III.^[46] These lengthy sequences generate substantial waste, as solvents and reagents are consumed in excess, amplifying material inefficiency and increasing costs.^[47] Selectivity issues persist, particularly in achieving precise diastereocontrol during the assembly of polyketides and polyethers, where reactions frequently produce mixtures of diastereoisomers that demand chromatographic separation to isolate the desired product.^[48] Additionally, the reliance on protecting groups to manage functional group compatibility introduces further inefficiencies, as their selective installation and deprotection add steps and reduce yields in multi-stage syntheses.^[46] Scalability remains a major barrier, with laboratory-scale protocols often failing to translate to industrial production due to purification difficulties for polar, complex intermediates and the high cost of rare reagents or catalysts.^[49] While multigram quantities have been achieved in select cases, such as the 59 g synthesis of minovincine, broader access to sufficient material for biological evaluation or commercialization is limited by these factors.^[46] Environmental concerns are pronounced, as organic solvents account for more than 70% of waste in typical syntheses, and hazardous reagents like triflic anhydride or boron trifluoride etherate contribute to toxicity and disposal challenges.^[47] Furthermore, accessing specific stereoisomers often requires extra transformations, such as epimerization, complicating routes and underscoring the need for more divergent asymmetric methods.^[46]

Emerging Trends

In recent years, computational tools leveraging artificial intelligence (AI) and machine learning (ML) have revolutionized route prediction in total synthesis, enabling automated retrosynthetic analysis and optimization of synthetic pathways. Systems like ASKCOS, an open-source software suite developed at MIT, integrate ML models for retrosynthesis prediction, feasibility assessment, and condition recommendation, with its 2025 update incorporating advanced modules for scalable planning of complex molecules. Similarly, IBM's RXN for Chemistry platform has seen enhancements, including the integration of quantum chemical data to improve the reliability of reaction and retrosynthesis predictions, as demonstrated in 2023 studies on organic transformations like Williamson ether synthesis. These tools reduce the time required for route design from weeks to hours, facilitating the exploration of vast chemical spaces for drug-like compounds.^[50]^[51] Emerging applications of quantum computing further augment these capabilities by simulating reaction mechanisms at unprecedented accuracy, particularly for challenging transition states in organic synthesis. For instance, variational quantum eigensolver (VQE)-based methods extended in 2024 allow for the modeling of chemical reaction dynamics on noisy intermediate-scale quantum (NISQ) devices, providing insights into energy barriers that classical simulations struggle with for large molecules. This approach holds promise for designing novel reactions in total synthesis, such as stereoselective bond formations, by predicting outcomes beyond current computational limits.^[52] Biocatalysis and hybrid chemoenzymatic methods are gaining traction for forging key carbon-carbon (C-C) bonds with high selectivity, addressing limitations in traditional catalysis. Engineered cytochrome P450 enzymes, evolved through directed evolution, have enabled direct C-C bond formation via oxidative cross-coupling, as shown in 2022 studies where variants catalyzed biaryl synthesis with turnover numbers exceeding 1,000 under mild aqueous conditions. Directed evolution techniques, refined in recent protocols, enhance enzyme selectivity by iteratively mutating active sites and screening variants, achieving enantiomeric excesses over 99% for non-natural substrates in synthetic routes. These advancements allow integration of biocatalysts into multi-step syntheses, combining enzymatic precision with chemical versatility.^[53]^[54]^[55] Sustainable practices are increasingly embedded in total synthesis through green chemistry principles, emphasizing waste minimization and renewable feedstocks. Biocatalysts and continuous flow processing align with these tenets by enabling reactions at ambient temperatures with minimal solvent use; for example, immobilized enzymes in flow reactors have boosted yields in pharmaceutical intermediates by up to 50% while reducing energy consumption. Metal-free activation strategies, such as organocatalytic or photoredox-free methods, further promote sustainability by avoiding toxic metals, as evidenced in 2025 developments for C-H functionalization that achieve high atom economy without heavy metal residues. These approaches not only lower environmental impact but also scale efficiently for industrial applications.^[56]^[57]^[58] Looking ahead, total synthesis is poised to tackle "undruggable" targets—proteins like KRAS or MYC previously deemed inaccessible—through innovative modalities such as covalent inhibitors and degraders, with 2025 strategies incorporating pseudo-natural products to enhance binding affinity. The synthesis of advanced materials, including dendrimers for drug delivery, benefits from iterative divergent-convergent routes that yield monodisperse structures with precise branching, as optimized in solid-phase methods for scalable production. Convergence with synthetic biology, particularly cell-free systems, enables on-demand assembly of complex natural products; 2024 reconstitutions of biosynthetic pathways in cell-free extracts have produced polyketides with yields rivaling microbial fermentation, blurring lines between chemical and biological synthesis. These trends signal a paradigm shift toward integrated, efficient, and eco-friendly synthesis pipelines by 2030.^[59]^[60]^[61]

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Total Syntheses of Cinchona Alkaloids via Photoredox-Catalyzed Deoxygenative Arylation. Organic Letters 2023, 25 (24) , 4586-4591.
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The endeavor of total synthesis and its impact on chemistry, biology ...
Total synthesis replicates natural molecules, leading to new molecules for healthcare, tech, and agriculture, and is a key part of organic synthesis.
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The historic total synthesis of strychnine by Woodward 1 in 1954 represented a milestone in the field of organic synthesis. 2 Strychnine (C21H22N2O2) ranks ...Introduction · II. An Overview of the Synthetic... · III.Total Syntheses · VII.References
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[PDF] Catalytic asymmetric synthesis - Nobel Prize
Knowles and Noyori receive half the Prize for: “ their work on chirally catalysed hydrogenation reactions” and Sharpless is rewarded with the other half of the.
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Mar 2, 2021 · This review summarizes the selected examples reported in the past seven years where organocatalytic methods were employed to achieve the key ...
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[PDF] Elias James Corey - Nobel Lecture
6 The basic ideas of retrosynthetic analysis were used to design many other syntheses and to develop a comput- er program for generating possible synthetic ...
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The Corey-Seebach Reaction allows a reversal of the normal reactivity of acyl carbon atoms, which combine only with nucleophiles.
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Stereochemistry and mechanism of the Wittig reaction. Diasteromeric reaction intermediates and analysis of the reaction course.
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Mechanism of the Wittig reaction: the role of substituents at ...
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Mechanism and Origins of Stereoselectivity of the Aldol-Tishchenko ...
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The first practical method for asymmetric epoxidation
Total Synthesis of Dixiamycins A and B via a Late-Stage N–N Bond ... Mechanism of the Sharpless Epoxidation Reaction: A DFT Study. The Journal of ...Missing: equation | Show results with:equation
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As several excellent reviews examine the use of enzymes in this context, we will focus on two applications of enzymatic resolution in total synthesis: Mori's ...
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Quantum Chemical Data Generation as Fill-In for Reliability ...
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IBM RXN for Chemistry
Predict reactions, find retrosynthesis pathways, and derive experimental procedures with RXN for Chemistry.
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Based on the principles and metrics of green chemistry and sustainable development, biocatalysis is both a green and sustainable technology.
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Since then, solid-phase synthesis has been used to generate a large array of dendrimers with various chemical compositions such as oligonucleotide, carbohydrate ...
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Oct 29, 2024 · We report the successful cell-free reconstitution of two natural product biosynthetic pathways of divergent complexity and structural classes.