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Varroa destructor

Varroa destructor, commonly known as the Varroa mite, is an ectoparasitic mite belonging to the family Varroidae that primarily infests the Western honey bee, Apis mellifera.^[1] This mite, measuring 1–1.5 mm in length with a flat, oval body, originated as a parasite of the Eastern honey bee, Apis cerana, but successfully switched hosts to A. mellifera around the mid-20th century, likely in Asia during the 1950s.^[2] It represents the single most significant parasitic threat to managed honey bee colonies worldwide, contributing to widespread colony losses and economic impacts on apiculture.^[3] The biology of V. destructor involves a biphasic life cycle adapted to exploit honey bee colonies. In the phoretic phase, adult female mites attach to the bodies of foraging adult bees for dispersal and initial feeding, using their chelicerae and salivary stylets to pierce the bee's exoskeleton.^[1] During the reproductive phase, foundress females invade capped brood cells—preferring drone brood for higher reproductive success—where they feed and deposit eggs via arrhenotokous parthenogenesis, producing up to six offspring per cycle that mature over 6–11 days, with the female progeny mating with the male progeny inside the cell.^[1] Contrary to earlier assumptions, V. destructor primarily consumes the host's fat body tissue through extraoral digestion rather than hemolymph alone, leading to profound physiological disruptions including impaired lipid and protein metabolism, reduced immune responses, and heightened susceptibility to environmental stressors.^[3] The impacts of V. destructor extend beyond direct feeding, as the mite serves as an efficient vector for RNA viruses, most notably deformed wing virus (DWV), which it amplifies and transmits during feeding, exacerbating viral epidemics within colonies.^[2] Infested bees exhibit shortened lifespans, deformed wings, diminished foraging ability, and lowered reproductive fitness, while at the colony level, high mite loads (>3% infestation) correlate with rapid population decline and collapse without intervention.^[1] This parasite's genetic lability, including hybridization events and rapid evolution of acaricide resistance, complicates control efforts, though some honey bee stocks show natural or bred resistance through mechanisms like grooming and hygienic behavior.^[2] Global spread via trade and migratory beekeeping has made V. destructor ubiquitous in A. mellifera populations outside isolated regions, underscoring the need for integrated management strategies.^[2]

Taxonomy and Morphology

Taxonomy

Varroa destructor belongs to the phylum Arthropoda, class Arachnida, subclass Acari, order Mesostigmata, family Varroidae, and genus Varroa.^[4]^[5] This ectoparasitic mite was originally classified under Varroa jacobsoni following its description by Oudemans in 1904, but in 2000, Anderson and Trueman separated it as a distinct species based on mitochondrial DNA analysis revealing multiple haplotypes. The two species share approximately 99.7% nuclear genome sequence identity, yet differ sufficiently in reproductive compatibility with honey bee hosts to warrant distinction. The genome of V. destructor was sequenced in 2019, revealing close similarity to V. jacobsoni and insights into its parasitic adaptations.^[6] Evolutionary origins of V. destructor trace to Southeast Asia, where it co-evolved as an obligate parasite of the eastern honey bee, Apis cerana.^[7] The first documented host switch to the western honey bee, Apis mellifera, occurred around 1952 in eastern Russia, giving rise to the Korean haplotype (also known as the Russia or K1 lineage), which became highly invasive. A similar event in 1957 produced the Japanese haplotype (J or K2 lineage), though less widespread globally. These two haplotypes represent the primary strains capable of reproducing on A. mellifera, driving the mite's global spread.^[8] Genetic diversity in invasive V. destructor populations remains low, attributed to serial bottlenecks during colonization, haplodiploid sex determination (where unfertilized eggs develop into haploid males), and prevalent sibling mating within brood cells.^[9] This reproductive strategy limits gene flow and heterozygosity, with most variation confined to mitochondrial DNA markers such as the cytochrome c oxidase subunit I (COI) gene, enabling haplotype identification.^[10] Prior to 2000, taxonomic confusion with V. jacobsoni obscured the precise timelines of introductions, as reports of "Varroa" infestations worldwide were not differentiated by species or haplotype.^[11]

Morphology and Identification

Varroa destructor is an ectoparasitic mite characterized by distinct morphological features across its life stages, which facilitate identification in apicultural and research settings. The adult female, the most commonly observed stage, exhibits a reddish-brown to dark brown coloration and an oval, dorsoventrally flattened body adapted for attachment beneath honey bees. This body measures 1.0–1.8 mm in length and 1.5–2.0 mm in width, with a curved dorsal shield that allows it to wedge between the host's abdominal plates.^[12]^[13] The female possesses eight legs bearing ambulacra with strong claws for clinging to the bee's exoskeleton, and chelicerae specialized as piercing stylets for feeding on host fat body tissue via extraoral digestion; notably, it lacks eyes, relying instead on chemosensory structures for host location.^[14]^[12]^[3] The mite undergoes several developmental stages within the sealed brood cell of its host. The protonymph emerges from the egg as a non-feeding, transparent white larva approximately 0.3 mm long, featuring eight short legs, pointed chelicerae, and no discernible sexual differences without dissection.^[12] It molts into the deutonymph stage, which is larger (up to 0.8 mm), white to pale tan, and actively feeding on the brood's fat body; this stage develops genital papillae and ambulacra but remains confined to the cell unless dispersal occurs rarely.^[12]^[15] Adult males, arising from unfertilized eggs, are pale, smaller (0.7 mm long by 0.6 mm wide), and non-feeding, with a more rounded, triangular body shape, longer legs relative to body size, and degenerate chelicerae unsuitable for piercing; they are short-lived and never leave the brood cell.^[11]^[12] Sexual dimorphism is pronounced, with females being larger, more pigmented, and robust for phoresy and reproduction, while males are pale, diminutive, and adapted solely for mating within the cell.^[15]^[11] Identification of V. destructor relies on these morphological traits, particularly the chelicerae and ambulacra, which distinguish it from other bee mites like Tropilaelaps species through microscopic examination.^[14] However, due to its close similarity to Varroa jacobsoni, definitive species confirmation often requires DNA-based methods, such as PCR amplification of mitochondrial markers like cytochrome c oxidase subunit I, to differentiate the two cryptic species.

Distribution and Hosts

Geographic Distribution

Varroa destructor is native to Southeast Asia, where it primarily parasitizes the eastern honey bee, Apis cerana, with the earliest description recorded in Java in 1904.^[16] The mite's host shift to the western honey bee, Apis mellifera, occurred following the introduction of this species to Asia in the mid-20th century, enabling its initial spread beyond its native range.^[17] The first documented invasion into Europe began in the 1960s, starting in eastern regions such as Bulgaria in 1963 and spreading westward through the Soviet Union and into Western Europe by the 1970s and 1980s.^[18] In the Americas, the mite arrived in South America around 1971, likely via Brazil, and reached North America in the late 1970s to early 1980s, with confirmed detections in the United States by 1987.^[18] By the 1990s, it had established across much of Asia outside its native range, North and South America, Europe, and parts of Africa, including North Africa around 1990 and South Africa in 1997, affecting at least 31 African countries by 2020.^[18] The global dissemination of V. destructor has been facilitated primarily by human activities, including the international trade of honey bee colonies, queens, and contaminated equipment, as well as natural dispersal through swarming and drifting bees.^[16] Migratory beekeeping practices have accelerated its spread within continents, while long-distance transport via ships or aircraft has enabled intercontinental invasions.^[16] Historical data prior to 2000 is complicated by widespread misidentification of the mite as Varroa jacobsoni, a related species; molecular studies in 2000 clarified V. destructor as the primary invader of A. mellifera hives worldwide, refining our understanding of its invasion pathways.^[18] By 2020, V. destructor was established on every continent except isolated regions, with widespread presence in North and South America, Europe, Asia, and much of Africa.^[18] As of 2025, it remains absent from certain isolated areas, including Oman, the Democratic Republic of the Congo, Malawi, and some Pacific islands (such as the Chatham Islands), due to stringent quarantine protocols.^[19] A significant recent development occurred in Australia, where the mite was first detected in New South Wales in June 2022 at the Port of Newcastle, leading to an initial emergency eradication effort that transitioned to long-term management by September 2023 due to confirmed establishment.^[20] By November 2025, infestations had spread to New South Wales, Queensland, Victoria, South Australia, and the Australian Capital Territory, prompting a transition to a national management program to build resilience until at least February 2026.^[21]^[22]^[23]^[20]^[24]

Primary and Secondary Hosts

Varroa destructor primarily parasitizes the Western honey bee (Apis mellifera), a host to which the mite shifted in the mid-20th century, resulting in high susceptibility due to the absence of long-term co-evolutionary adaptations. This mismatch allows the mite to reproduce successfully in both drone and worker brood cells of A. mellifera, leading to rapid population growth and severe colony damage. In contrast, the original and secondary natural host, the Eastern honey bee (Apis cerana), exhibits tolerance through behavioral defenses such as grooming and hygienic brood removal, which limit mite reproduction primarily to drone brood cells. Experimental infestations and field observations indicate that V. destructor can parasitize other Apis species, including Apis dorsata, where both phoretic and reproductive phases occur, with nymph stages observed in brood cells confirming successful reproduction.^[25] The mite has also been recorded in the phoretic phase on bumblebees (Bombus spp.), but reproduction is absent on non-Apis hosts due to incompatible brood development cycles. Within A. mellifera colonies, V. destructor shows a strong preference for drone brood over worker brood, infesting drone cells at rates 8–10 times higher, attributed to the longer pre-capping period (40–50 hours versus 15–20 hours) that allows more time for invasion and higher reproductive success. During the phoretic phase, mites preferentially attach to nurse bees, which frequently visit brood cells, facilitating transport to suitable reproductive sites. The co-evolutionary history with A. cerana has fostered a balanced host-parasite relationship, where mite populations remain low through host defenses, whereas A. mellifera lacks these mechanisms, resulting in exponentially higher infestation rates and vulnerability.

Life Cycle and Reproduction

Phoretic Phase

The phoretic phase represents the non-reproductive dispersal stage in the life cycle of Varroa destructor, during which adult female mites attach to honey bees for transport within and between colonies. This phase typically lasts 4.5 to 11 days when brood is present during the brood-rearing season, allowing mites to seek opportunities for reproduction; however, in the absence of brood during winter, it can extend up to five to six months as mites enter a state of diapause.^[12] Mites preferentially cling to nurse bees, which frequent brood areas, using specialized ambulacral structures on their pretarsi for secure attachment to the bee's exoskeleton, often on the abdomen or thorax.^[26]^[27] During this period, mites feed sporadically on the host's fat body tissue by piercing the intersegmental membrane and using extraoral digestion to liquefy and consume the tissue, sustaining themselves while minimizing host detection.^[3] This phoretic attachment facilitates the mite's dispersal role, enabling spread from hive to hive through natural bee behaviors such as drifting (unintentional movement between colonies), robbing (foraging on weakened hives), and swarming (colony reproduction).^[12] Mites detach from their host upon the bee's death, rapidly seeking a new carrier to avoid starvation, which underscores the phase's reliance on host vitality for mite survival.^[26] In winter, reduced bee activity and clustering lead to higher mite mortality, as fallen hosts provide fewer opportunities for reattachment compared to active foraging seasons.^[28] The average lifespan of phoretic female V. destructor is approximately 27 days when brood is available, reflecting the phase's integration with reproductive opportunities; without brood access, longevity extends but at the cost of increased mortality risks.^[26] Transition to the reproductive phase occurs when a mite detaches from its phoretic host to invade an uncapped brood cell suitable for oviposition.^[12]

Reproductive Phase

The reproductive phase of Varroa destructor occurs within the capped brood cells of honey bees, where a fertile female mite, known as the foundress, invades and completes her reproductive cycle. The foundress enters an uncapped brood cell containing a fifth-instar larva, typically 15–20 hours before capping in worker cells or 40–50 hours before capping in drone cells, allowing her to become trapped with the developing pupa upon sealing.^[29] This invasion is selective, with the mite preferring drone brood due to its larger size, longer developmental period, and attractive chemical cues from the larva.^[29]^[30] Once the cell is capped, the foundress activates her ovaries after feeding on the fat body tissue of the host larva and lays her first unfertilized egg, which develops into a male, approximately 60–70 hours post-capping.^[29]^[3] She then deposits 3–5 fertilized eggs at intervals of about 30 hours each, resulting in a total of 4–6 eggs per cycle, with subsequent eggs producing females.^[29] Development from egg to adult takes 5.8 days for females and 6.6 days for males, enabling the production of 2–5 mated daughter mites per foundress under optimal conditions.^[29] The foundress exhibits maternal care by creating a single feeding site—a wound in the pupa's cuticle, usually on the fifth abdominal segment—through which all offspring, including herself, access the host's fat body tissue via extraoral digestion.^[29]^[3] She positions her offspring near this site to facilitate their feeding, though the nymphs consume host fluids directly rather than through regurgitation.^[11] Males emerge first and remain in the cell, mating with their emerging sisters at the fecal site using spermatophores; mating bouts last 3–6 minutes, and males typically die shortly after, while mated females prepare to emerge with the host adult.^[29]^[30] Fecundity varies by host brood type, with up to 5 offspring possible in worker cells (yielding 1.3–1.7 viable daughters on average) and up to 6–7 in drone cells (yielding 2–3 viable daughters), owing to the extended development time in drones that permits more egg-laying opportunities.^[29] Reproductive success is notably higher in Apis mellifera than in the original host Apis cerana, where reproduction is largely restricted to drone cells due to shorter worker brood cycles, aggressive grooming, and rapid cell uncapping behaviors that suppress mite proliferation.^[29]^[30]

Pathogenesis and Interactions

Feeding and Physiological Effects

Varroa destructor employs its chelicerae to pierce the soft intersegmental membrane of the honey bee's abdomen, creating an open wound through which it injects saliva containing anticoagulants and enzymes to suppress host healing and facilitate feeding.^[31] The mite primarily consumes fat body tissue rather than hemolymph, using a muscular pharynx to ingest fluid at a rate of approximately 1 μl per day based on excretion volume measurements.^[3]^[32] This feeding occurs repeatedly on the same site, maintaining the wound open for extended periods during both phoretic and reproductive phases.^[33] Feeding by V. destructor directly impairs honey bee physiology, leading to reduced body weight and impaired immune responses in infested individuals.^[34] Adult workers parasitized by the mite exhibit shortened lifespans, with studies showing up to a 50% reduction compared to uninfested bees.^[35] These effects compromise foraging efficiency and overall colony labor force.^[36] In brood, V. destructor infestation causes retarded pupal development and diminished size of hypopharyngeal glands in newly emerged nurse bees, reducing their capacity for royal jelly production.^[28] Infested pupae experience slowed growth and lower emergence weights, exacerbating nutritional deficits across generations.^[37] Even low infestation levels, such as 1–2 mites per brood cell, inflict significant physiological harm, including elevated mortality and sublethal impairments.^[38] Over multiple generations, these cumulative effects weaken the entire colony by progressively diminishing bee vitality and reproductive output, ultimately leading to population decline.^[11] V. destructor exhibits a preference for drone brood, amplifying damage in this reproductive stage.^[39] Infested brood often develop high levels of deformed wing virus (DWV), leading to physical deformities such as shortened abdomens and malformed wings in emerging adults.^[34]

Transmission Dynamics

Varroa destructor spreads within honey bee colonies primarily through phoresy, where adult female mites attach to worker bees, particularly nurse bees, to facilitate movement across the hive. During the phoretic phase, mites preferentially infest middle-aged nurse bees that tend to late-stage brood, enabling them to access suitable brood cells for reproduction and thereby perpetuating intra-colony transmission.^[40] High transmission rates occur in dense clusters of nurse bees, where close physical contact among bees increases opportunities for mite transfer between individuals.^[41] Additionally, interactions during grooming behaviors, such as autogrooming or allogrooming, can inadvertently contribute to mite dispersal if mites are not fully dislodged, allowing them to reattach to nearby bees.^[42] Between colonies, V. destructor is transmitted via several mechanisms involving bee mobility and human activities. Drifting worker bees, which mistakenly enter neighboring hives, carry phoretic mites and significantly contribute to inter-colony spread, especially in apiaries with high colony densities.^[43] Robbing bees from stronger colonies invade weakened ones, acquiring mites in the process and transporting them back to their home hives, a phenomenon exacerbated during periods of resource scarcity.^[43] Swarming events also facilitate transmission, as emerging swarms can distribute a portion of the mite population from the parent colony to new sites.^[44] Contaminated beekeeping equipment, such as hives or tools moved between apiaries without proper sanitation, serves as another vector for mite transfer.^[45] Secondary vectors include flowers, where dislodged mites can survive briefly and reattach to foraging bees from different colonies.^[46] The rate of V. destructor infestation is influenced by seasonal factors and colony dynamics, leading to rapid population increases during active brood-rearing periods. In summer, mite populations can double every 3–4 weeks due to multiple reproductive cycles synchronized with honey bee brood production, resulting in exponential growth tied to the availability of brood cells.^[47] This growth is descriptive of the mite's reproductive potential, where each cycle amplifies the phoretic population for further dispersal. During winter, clustering of bees reduces overall mite mobility but concentrates transmission within the tight-knit bee mass, potentially sustaining infestations despite lower brood availability.^[48]

Associated Diseases and Impacts

Virus Transmission

_Varroa destructor serves as a primary vector for several honey bee viruses, most notably Deformed wing virus (DWV), Acute bee paralysis virus (ABPV), and Kashmir bee virus (KBV). These viruses, along with at least five others such as Israeli acute paralysis virus (IAPV) and Slow bee paralysis virus (SBPV), are transmitted through two main routes: fecal-oral contamination during mite defecation on brood cells or adult bees, and direct injection into the host's hemolymph during feeding. Transmission efficiency varies by virus; for instance, KBV-positive mites transfer the virus to bee pupae in approximately 70% of cases, while virus-free mites can acquire KBV through close contact with infected mites.^[49]^[50]^[51] The mechanism of transmission begins with mites acquiring viruses from the hemolymph of infected bees during feeding. Subsequently, the mites inject viral particles directly into new hosts via their salivary secretions, bypassing external barriers and enabling rapid systemic infection. For DWV specifically, the virus replicates within the mite's salivary glands, amplifying viral titers up to 357-fold and facilitating higher doses during subsequent feedings on developing pupae. This injection route contrasts with purely oral transmission and enhances virulence, as evidenced by studies showing active DWV replication in V. destructor, altering evolutionary dynamics and increasing viral loads in both mites and bees.^[52]^[53]^[54] Varroa-mediated virus transmission induces immunosuppression in honey bees by depleting hemolymph resources and upregulating viral replication, weakening innate immune responses such as antimicrobial peptide production. Prior to V. destructor's global spread, these viruses typically caused benign or low-level infections in honey bees; however, mite vectoring has transformed them into highly pathogenic agents, leading to severe symptoms like wing deformities and paralysis. In heavily infested colonies, this synergy results in elevated mortality, with DWV-positive colonies harboring ≥1 mite per 100 bees facing 3.46 times higher winter mortality odds compared to less infested ones, and transmission rates for certain viruses reaching 50–90% in pupae.^[28]^[55]^[56]^[50] Recent 2025 research highlights co-transmission dynamics of IAPV with DWV, revealing variable mortality outcomes in co-infected bees depending on seasonal factors and mite infestation levels, underscoring the mite's role in exacerbating multi-virus epidemics. Currently, no commercial vaccines exist for these bee viruses, though experimental approaches like RNA interference (RNAi) targeting viral genes—such as DWV structural proteins—have shown promise in reducing infection loads and bee mortality in lab and field trials.^[57]^[58]^[59]^[60]

Contribution to Colony Collapse Disorder

_Varroa destructor plays a central role in colony collapse disorder (CCD) by acting as a primary driver through the escalation of viral infections in honey bee colonies. The mite's feeding suppresses bee immunity, facilitating the rapid spread of debilitating viruses that lead to symptoms characteristic of CCD, including the sudden disappearance of adult worker bees and neglect of brood care. Research indicates that infestations exceeding 3% of adult bees correlate strongly with the onset of these collapse symptoms, as high mite loads overwhelm colony resilience and trigger cascading health failures.^[61]^[62] CCD is a multifactorial syndrome where Varroa interacts synergistically with other stressors such as pesticide exposure and nutritional deficiencies to precipitate colony failure. Studies demonstrate that Varroa-free honey bee populations experience CCD at much lower rates compared to infested ones, underscoring the mite's pivotal contribution to the disorder's severity. For instance, in Australia prior to the 2022 detection of Varroa, colony collapse events resembling CCD were rare, highlighting the mite's necessity for the syndrome's full manifestation.^[63]^[64]^[65] The surge in CCD cases beginning in 2006 across the United States and Europe coincided with the widespread establishment and intensification of Varroa infestations in managed hives. Recent 2025 data from U.S. beekeeping surveys report over 60% colony losses in commercial operations, with unmanaged Varroa infestations identified as a key factor in these declines, particularly amid emerging mite resistance to common acaricides. Economically, Varroa-driven losses threaten billions in annual U.S. agricultural value, as honey bee pollination supports over $15 billion in crop production; unmanaged infestations amplify these stressors, resulting in hundreds of millions in direct replacement and production costs each year.^[66]^[67]^[68]^[69]^[70]

Control and Management Strategies

Monitoring Methods

Monitoring Varroa destructor infestations is essential for integrated pest management in honey bee colonies, as timely detection allows beekeepers to intervene before mite populations reach damaging levels. Common techniques focus on sampling adult bees or observing natural mite fall to estimate infestation rates, typically expressed as the percentage of mites per 100 bees or daily drop counts. These methods balance accuracy, cost, and non-lethality, with recommendations to sample at least 300 bees or monitor multiple colonies per apiary for reliable data.^[71]^[72] Sticky boards placed under screened bottom boards capture naturally falling mites, providing a non-invasive estimate of infestation through daily or 24-hour drop counts. For a standard 10-frame hive, a threshold exceeding 59 mites per 24 hours indicates the need for action, as this correlates with rising populations during brood-rearing periods. This method is simple and inexpensive but less precise for low-level infestations, as it misses phoretic mites still attached to bees.^[72] The alcohol wash remains the gold standard for accuracy, involving the submersion of a 300-bee sample in 70% isopropyl alcohol or soapy water, followed by shaking and straining to count dislodged mites, achieving near-complete recovery rates often exceeding 95% compared to less reliable alternatives. A typical threshold is 3 mites per 100 bees, though seasonal adjustments apply: below 1% in spring and 2% during the rest of the year to maintain colony health. While lethal to the sample, its precision makes it ideal for quantifying mean mite abundance in apiaries with many colonies, where sampling 20% of hives suffices.^[73]^[74]^[72] For a non-lethal option, the powdered sugar roll coats a 300-bee sample with two tablespoons of powdered sugar, dislodging mites through vigorous shaking over a mesh screen for counting, with a similar 2% threshold for treatment. This technique recovers approximately 90% of mites relative to alcohol washes but is affected by factors like humidity and nectar flow, making it less reliable in certain conditions. In-hive brood frame inspections complement these by visually checking for mites on pupae, particularly in drone brood where infestations are higher due to extended development times.^[74]^[72]^[71] Monitoring should occur monthly during peak brood-rearing seasons (spring through fall) to track population dynamics, with economic thresholds of 1–3% infestation rates aimed at preventing winter colony losses that can exceed 50% in unmanaged apiaries. These levels tie to V. destructor's reproductive potential, where early detection avoids exponential growth.^[73]^[72]^[71] Recent advances in 2025 include AI-powered digital apps and scanners for automated counting, such as the BeeVS system, which analyzes images from sticky sheets using neural networks to detect mites with error rates below 1% for loads over 10 mites, enabling rapid, repeatable assessments across large apiaries. Predictive digital twin platforms integrate wireless sensors and computer vision (e.g., ViT models with 98.6% accuracy) for real-time infestation forecasting and what-if scenario planning, reducing manual labor while supporting scalable management.^[75]^[76]

Chemical Control

Chemical control of Varroa destructor primarily involves synthetic and organic acaricides applied to honey bee colonies to reduce mite populations. These treatments target phoretic and reproductive mites but require careful management to maintain efficacy and minimize risks to bees and hive products. Common approaches include strips, pads, dribbles, and vapors, with selection influenced by regional regulations, mite resistance levels, and seasonal conditions.^[73] Amitraz, a synthetic formamidine acaricide delivered via plastic strips (e.g., Apivar), remains a widely used treatment with reported efficacies up to 95% under optimal conditions. However, resistance has emerged globally, including in the United States, where the Y215H mutation in the mite's octopamine receptor has been associated with resistance in a high proportion (e.g., 88.5%) of samples from resistant populations, rendering amitraz ineffective in affected hives.^[77]^[78] Rotation with other modes of action is essential to delay further resistance development.^[79] Organic acids like formic acid and oxalic acid offer effective alternatives with lower resistance risks. Formic acid, applied via pads or vapor (often combined with propolis for stability), achieves 80–90% mite kill rates, though efficacy varies with temperature and brood presence.^[80] Oxalic acid, administered as a dribble or vapor, yields 90–99% efficacy specifically in broodless periods, such as winter, but shows reduced performance with active brood.^[73] No widespread resistance to these acids has been confirmed, making them valuable for integrated programs.^[81] Pyrethroids, such as tau-fluvalinate (e.g., Apistan strips), were among the first chemical controls introduced but are now largely ineffective due to resistance that developed in the 1990s across Europe, including in the Czech Republic and United Kingdom, where point mutations in the mite's sodium channel gene confer tolerance.^[82]^[83] Their use is discouraged in resistant areas to avoid selecting for additional mutations. Application timing is critical for maximizing impact and minimizing bee stress; for instance, oxalic acid treatments are most effective during broodless phases to target phoretic mites.^[73] Rotating acaricides with distinct mechanisms—such as alternating amitraz with organic acids—helps manage resistance, as recommended by integrated pest management guidelines.^[79] Organic approvals for formic, oxalic, and thymol-based products vary by region, with many certified for use in Europe and North America under standards like EU organic regulations.^[84] Potential risks include chemical residues in honey and wax, which can accumulate from repeated applications and affect bee health or marketability; for example, amitraz residues have been detected in comb wax at levels up to 0.1 mg/kg post-treatment.^[85] Recent thymol-based formulations, such as low-dose nanoemulsions, have gained traction as resistance-mitigating alternatives, offering 70–90% efficacy with reduced residue concerns compared to synthetics.^[86]^[87]

Physical and Cultural Controls

Physical controls for Varroa destructor involve mechanical interventions that exploit the mite's biology to prevent reproduction or facilitate removal without chemical agents. Screened bottom boards replace solid hive floors with mesh screens, allowing phoretic mites to fall out of the hive while retaining heat and preventing re-entry, thereby reducing mite populations in the brood. Studies indicate these boards can decrease mite levels by up to 25% when used consistently, though efficacy varies with climate and must be paired with other methods to prevent economic thresholds from being exceeded.^[88] Drone comb traps leverage the mite's preference for drone brood, where females produce up to 2.6 offspring per cell compared to 1.4 in worker cells. Beekeepers insert drone foundation frames into the brood nest during spring or summer, allowing the queen to lay drone eggs; once capped (around days 18-24), the frame is removed and the infested brood destroyed by freezing or burning. This method typically reduces mite populations by 40-50% over two cycles per season and up to 90% when combined with a broodless period.^[89] Cultural practices focus on hive management to disrupt mite reproduction cycles and limit spread. Brood breaks, induced by caging the queen for approximately three weeks using devices like Scalvini cages, halt larval production and force mites into the vulnerable phoretic stage on adult bees, where they are more susceptible to natural grooming or removal. This approach can significantly lower mite numbers by slowing reproduction, with recovery typically occurring within two months, though it requires careful timing to avoid stressing the colony.^[73]^[90] The use of small-cell combs, with cell diameters reduced to 4.9 mm from the standard 5.4 mm, aims to shorten the post-capping period and potentially increase immature mite mortality due to spatial constraints. However, scientific evaluations have found no significant impact on total brood area, mites per brood cell, or overall mite populations, with Varroa levels remaining comparable to standard combs across trials. Efficacy remains disputed, offering minimal benefit under typical conditions.^[91] Promoting bee hygiene through cultural means enhances natural grooming behaviors to dislodge mites. Applying powdered sugar dust to bee clusters stimulates auto-grooming, prompting workers to remove and drop mites from their bodies, providing a non-invasive way to reduce infestations in lightly affected colonies. Additionally, increasing apiary spacing to at least 100 meters between hives minimizes bee drift, which spreads phoretic mites; closer spacings (0-10 meters) result in 50-60% higher average mite counts due to increased inter-colony movement.^[92]^[93] These physical and cultural controls are most effective when integrated into a monitoring program, such as regular alcohol washes or sticky board counts to keep infestations below 2 mites per 100 bees, particularly in low-infestation scenarios. While labor-intensive due to frequent interventions like frame removals or queen caging, they support sustainable management by reducing reliance on other strategies and maintaining colony health without residues.^[73]

Biological and Genetic Controls

Biological controls for Varroa destructor primarily involve leveraging natural enemies or enhancing host resistance through selective breeding of honey bees. Hygienic stocks, such as the Minnesota Hygienic line, have been developed to promote the removal of mite-infested pupae by worker bees, achieving removal rates exceeding 40% in naturally infested brood cells, which contributes to a substantial reduction in mite populations.^[94] Similarly, Varroa Sensitive Hygiene (VSH) breeding programs select for bees that uncap and remove infested worker brood, with efficacy reaching up to 70% in disrupting mite reproduction under controlled conditions, as demonstrated in USDA evaluations where VSH colonies maintained low infestation levels without chemical interventions for over two decades.^[95] These traits are heritable and integrated into commercial breeding lines, though they require ongoing selection to maintain expression amid genetic drift.^[96] Genetic approaches target the mites directly using RNA interference (RNAi), where double-stranded RNA (dsRNA) sprays silence essential mite genes, such as calmodulin, leading to up to 50% reduction in female mite reproduction in laboratory trials by inhibiting egg laying and progeny viability.^[97] Field applications, including sugar syrup feeders delivering dsRNA targeting genes like acetyl-CoA carboxylase, have shown 33–42% reductions in phoretic mite infestations compared to controls, with no adverse effects on bee health.^[98] CRISPR-Cas9 editing remains experimental as of 2025, primarily used to validate resistance mutations in mite genes like the β-adrenergic-like octopamine receptor, but holds potential for future targeted disruptions in mite reproduction without affecting bees.^[99] Other biological agents, such as entomopathogenic fungi, offer limited field success. Strains of Metarhizium anisopliae, applied via spore-coated strips or dusts, achieve 20–50% mite mortality through mycosis in honey bee colonies, with evolved variants (e.g., JH1078) providing control comparable to mild chemical treatments for up to five weeks, though efficacy varies with temperature and hive humidity.^[100] Predatory mites like Stratiolaelaps scimitus have been tested for direct predation on V. destructor, but remain unviable for widespread use due to inconsistent establishment in hives and minimal long-term population suppression.^[101] As of 2025, RNAi-based varroacides like Norroa (vadescana dsRNA) have been registered by the EPA following U.S. field trials, demonstrating up to 18 weeks of mite control and improved colony health in multi-regional studies.^[102]^[103] Breeding programs in the U.S. (e.g., USDA VSH and low-Varroa growth lines) and Europe (e.g., native subspecies selection) have yielded 20–30% of tested lines with sustained resistance, characterized by lower infestation rates and reduced deformed wing virus prevalence under natural selection pressures.^[104] These efforts emphasize integrated genetic resistance to minimize reliance on short-term interventions.^[105]

References

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Varroa destructor: how does it harm Apis mellifera honey bees and ...
Jun 15, 2020 · Despite the extensive literature on the mite (see [10–12] for reviews), its biology and impact on its honey bee host remain partially unknown.
[2]
https://www.cell.com/trends/parasitology/fulltext/S1471-4922(20)30101-X
[3]
Varroa destructor feeds primarily on honey bee fat body tissue and ...
Jan 15, 2019 · Varroa destructor causes considerable damage to honey bees and subsequently the field of apiculture through just one process: feeding.
[4]
Varroa destructor - NCBI - NIH
Varroa destructor, also known as honeybee mite or honeybee ectoparasitic mite, is a species with Taxonomy ID 109461.
[5]
Varroa destructor | NatureServe Explorer
Classification ; Phylum: Arthropoda ; Class: Arachnida ; Order: Mesostigmata ; Family: Varroidae ; Genus: Varroa.
[6]
Divergent evolutionary trajectories following speciation in two ...
Oct 1, 2019 · The first record of Varroa mite parasitism dates back to 1904 in Java in where female mites were described from an Indo-Malayan Apis cerana ...
[7]
Varroa destructor: A Complex Parasite, Crippling Honey Bees ...
The parasitic mite, Varroa destructor, has shaken the beekeeping and pollination industries since its spread from its native host, the Asian honey bee (Apis ...
[8]
The invasive Korea and Japan types of Varroa destructor ... - NIH
Only two out of several known mitochondrial haplotypes of V. destructor have been found to be capable of reproducing on A. mellifera (Korea and Japan).Missing: 1952 | Show results with:1952
[9]
Fine scale population genetic structure of Varroa destructor, an ...
Three factors contribute to this low diversity: genetic bottleneck events, haplodiploid sex determination, and a unique life history resulting in a majority of ...
[10]
[PDF] Genetic characterization of the mite Varroa destructor (Acari
Aug 18, 2009 · Anderson and Trueman (2000) using mtDNA markers also verified the biotypes as the Korean (= Russian) and Japanese haplotypes. The results of the ...
[11]
Biology and Management of Varroa destructor (Mesostigmata ...
Jan 5, 2020 · Taxonomy. Varroa destructor was initially described by A. C. Oudemans in 1904 as V. jacobsoni (Oudemans 1904), and eventually re-described as V.
[12]
Varroa, Varroa destructor Anderson and Trueman (Arachnida: Acari
The adult female mites are reddish-brown to dark brown and oval in shape, measuring 1.00 to 1.77 mm long and 1.50 to 1.99 mm wide. Their curved bodies fit into ...
[13]
Varroa destructor (Varroa mite) | CABI Compendium
Varroa destructor and Varroa jacobsoni (Acari: Varroidae) are thought to be closely related (Zhang, 2000; Delaplane, 2001), both parasitizing the Asian honey ...Missing: classification | Show results with:classification<|control11|><|separator|>
[14]
[PDF] Standard methods for varroa research Métodos estándar de la ...
Adult females are large (about 1.5 mm in width) and reddishbrown in colour, whereas males and female nymph stages are smaller and cream or white in colour. All ...
[15]
Morphological identification, prevalence, and infestation rate of ...
3, The adult males and females of V. destructor are different based on body size, shape, legs, and color. The body size of females is larger than males. The ...
[16]
Varroa destructor : USDA ARS
Dec 1, 2017 · The adult female Varroa is oval and flat, about 1.1 mm long and 1.5 mm wide, pale to reddish-brown in color, and can be seen easily with the ...Missing: identification key
[17]
Geographical Distribution and Selection of European Honey Bees ...
Dec 8, 2020 · The parasitic mite Varroa destructor spread throughout Europe in the second half of the last century, and now represents the greatest problem ...2.2. 1. Apis Cerana · 2.4. 3. Varroa Sensitive... · 2.4. 11. Varroa Versus Virus...Missing: timeline | Show results with:timeline
[18]
https://doi.org/10.3390/insects11120873
[19]
Varroa mite transition to management
Varroa mite (Varroa destructor) was first detected in DPIRD surveillance hives at the Port of Newcastle in June 2022. The Varroa mite Response was ...
[20]
https://www.dpi.nsw.gov.au/animals-and-livestock/bees/varroa-mite-emergency-response
[21]
In which countries is Varroa a threat? - PerfectBee
Jul 24, 2025 · The species is not currently present in Oman, Congo, Democratic Republic of Congo, and Malawi. Please keep up to date on your country's ...Missing: distribution Pacific
[22]
https://pir.sa.gov.au/biosecurity/animal_health/animal_species/bees/varroa_mite
[23]
Varroa Mite Reproductive Biology - Bee Health
Aug 20, 2019 · Varroa mite life cycle has two stages (Fig. 1). During the phoretic stage, mites ride on adult workers or drones, at the same time feeding on blood (hemolymph) ...Missing: morphology | Show results with:morphology
[24]
[PDF] ADHESION MECHANISMS OF VARROA DESTRUCTOR ONTO APIS
As a phoretic mite, varroa has to have good pretarsal apparatus (called the ambulacrum) for locomotion, grasping and escaping grooming behaviour of the bee.Missing: cling | Show results with:cling
[25]
Varroa destructor and its impacts on honey bee biology - Frontiers
destructor females adhere and parasitize adult bees, which enable them to be transported to worker or drone brood cells where they reproduce, and to spread ...Introduction · Varroa destructor genetic... · Varroa destructor life cycle and...
[26]
[PDF] Biology and control of Varroa destructor
Biology and control of Varroa destructor. Peter Rosenkranz a,*, Pia Aumeier b, Bettina Ziegelmann a a University of Hohenheim, Apicultural State Institute ...Missing: text | Show results with:text
[27]
Understanding the Enemy: A Review of the Genetics, Behavior ... - NIH
Feb 7, 2022 · Varroa mites typically invade a cell individually, leading to the offspring of one foundress to mate with one another. However, multiple ...<|control11|><|separator|>
[28]
Varroa destructor from the Laboratory to the Field - PubMed Central
Varroa destructor mating biology leads to intense inbreeding with no exchange among lineages in the population while a single foundress invades a brood cell.
[29]
Insights into the metabolism and behaviour of Varroa destructor ...
The weight and volume of excretions suggest mites can consume nearly a microlitre of host fluids each day. Compounded over 10 days, this together with open ...
[30]
Effect of Varroa destructor, Wounding and Varroa Homogenate ... - NIH
Jan 12, 2017 · destructor can maintain open wounds in the honey bee integument and repeatedly feed on the same open wounds over a long period of time, and by ...
[31]
How Varroa Parasitism Affects the Immunological and Nutritional ...
Specifically, Varroa-infested bees have reduced weight and lifespan, elevated incidence of deformed wings and related phenotypes (shortened abdomen, ...Missing: shorter | Show results with:shorter
[32]
[PDF] Biology of the varroa mite - Veto-pharma
Reduced lifespan of bees (-50%) and higher risk of colony collapse, especially during winter. 1 - Ramsey SD, vanEngelsdorp D. Varroa destructor feed primarily ...<|control11|><|separator|>
[33]
Varroa destructor infestation impairs the improvement of landing ...
Sep 9, 2020 · Varroa destructor infestation of a honeybee colony impairs performance of foragers. It results in a more prolonged absence from the colony ...
[34]
Interactive effect of reduced pollen availability and Varroa destructor ...
Both protein level and growth are expected to be reduced when bee prepupae were infested with V. destructor and subsequently raised in cages with reduced pollen ...
[35]
Managing Varroa Mites in Honey Bee Colonies
The larvae and protonymphs of male and female mites look identical, but the deutonymphs and adults are easy to differentiate. The females are oval in shape, ...Missing: morphology | Show results with:morphology<|control11|><|separator|>
[36]
Genotype, but Not Climate, Affects the Resistance of Honey Bees ...
Jul 15, 2022 · Varroa destructor parasitism also inhibits the immune system of bees [6,7,8] and reduces honey yields of colonies [9]. Another condition that ...
[37]
Parasitic Mites - Honey Bee Health Guide
Varroa Mites. The varroa mite (Varroa destructor) is considered the greatest ... phoretic phase. Following recommended timing insures the product is ...
[38]
Honey bees increase social distancing when facing the ectoparasite ...
Oct 29, 2021 · Then, the dispersal of the parasite within the hive takes place by parasitizing mainly nurse bees (phoretic phase) (27). In this work, we ...<|separator|>
[39]
Grooming Behavior in Naturally Varroa-Resistant Apis mellifera ...
This study sheds light on how honey bee colonies can adaptively respond to mite pressure by modeling their behavior to resist Varroosis.Missing: intra- transmission drifting
[40]
Mite bombs or robber lures? The roles of drifting and robbing in ...
Jun 21, 2019 · The roles of drifting and robbing in Varroa destructor transmission from collapsing honey bee colonies to their neighbors. David Thomas Peck ...Missing: intra- grooming
[41]
(PDF) Distribution of Varroa destructor between swarms and colonies
Aug 5, 2025 · Bee colonies reproduce by colony division during swarming. In colonies infested by the parasitic mite, Varroa destructor, colony division will ...
[42]
Varroa Mites - Texas Apiary Inspection Service (TAIS)
Adult females are approximately the size of the head of a pin and oval-shaped. They are reddish brown in color and will darken with time. The adult female mites ...Missing: morphology | Show results with:morphology
[43]
Pathogen shifts in a honeybee predator following the arrival of the ...
Jan 9, 2019 · Here, we investigate DWV spillover in an invasive Hawaiian population of the wasp, Vespula pensylvanica, a honeybee predator and honey-raider.
[44]
Flowers as dirty doorknobs: Deformed wing virus transmitted ...
Aug 13, 2021 · In this study, we showed that DWV may be spread between bee genera through the shared use of flowers. Through mathematical simulation ...
[45]
[PDF] The Varroa Mite - Bayer
The nymphs are white and take six days to mature into adult female mites while passing through various development stages. Males need about seven days to mature ...
[46]
Varroa destructor re-invasion dynamics during autumn and winter in ...
Jan 25, 2023 · Infestation with Varroa destructor compromises the survival of Apis mellifera colonies, especially during the winter season.
[47]
[PDF] Transmission of Kashmir bee virus by the ectoparasitic mite Varroa ...
KBV-positive mites transmit the virus to bee pupae about 70% of the time, and virus-free mites can become positive by cohabiting with positive mites.
[48]
Honey Bee Viruses, the Deadly Varroa Mite Associates - Bee Health
Aug 20, 2019 · Varroa mites (Varroa destructor) are now the most serious pest of western honey bee colonies and one of the primary causes of honey bee decline.
[49]
The Acute bee paralysis virus-Kashmir bee virus-Israeli ... - PubMed
These viruses are frequently implicated in honey bee colony losses, especially when the colonies are infested with the parasitic mite Varroa destructor.
[50]
Deformed wing virus type A, a major honey bee pathogen, is ...
Aug 27, 2019 · In this study, we exposed Varroa mites to DWV type A via feeding on artificially infected honey bees. A significant, 357-fold increase in DWV load was observed ...
[51]
Virus replication in the honey bee parasite, Varroa destructor
Nov 15, 2023 · Varroa mites have drastically altered the honey bee viral landscape, as demonstrated during the spread of V. destructor throughout New Zealand, ...
[52]
[PDF] The Dynamics of Deformed Wing Virus Concentration and Host ...
Jan 8, 2019 · However, Varroa is an efficient vector and activator of DWV because virus particles are injected directly into bee hemolymph during mite ...
[53]
Varroa destructor from the Laboratory to the Field - MDPI
Here we review what has been done and what can be done from the laboratory to the field against the parasitic pressure held on honeybees.Varroa Destructor From The... · 3. Varroa Destructor... · 4. Biocontrol And Ipm...
[54]
Varroa destructor and deformed wing virus interaction increases ...
Varroa destructor and deformed wing virus interaction increases incidence of winter mortality in honey bee colonies.
[55]
Infection timelines and co-infection effects of Israeli acute paralysis ...
Oct 29, 2025 · Co-infected bees with DWV and IAPV result in variable mortality results depending on the season and that the two viruses do not act ...
[56]
https://pmc.ncbi.nlm.nih.gov/articles/PMC11232088/
[57]
Effects of Deformed Wing Virus-Targeting dsRNA on Viral Loads in ...
Nov 15, 2023 · Specifically, two studies have tested the use of RNAi to reduce the development of DWV infection in honey bees with promising results. Desai ...
[58]
RNAi and Antiviral Defense in the Honey Bee - PMC - NIH
Dec 21, 2015 · Similar results were obtained when larvae and adult bees fed dsRNA targeting Deformed wing virus (DWV) had reduced mortality, virus load, and ...
[59]
Impact of Varroa destructor and associated pathologies on the ...
Aug 6, 2025 · This study analyses the role of Varroa and other associated pathogens, such as viruses or the fungus Nosema ceranae, and their relationships ...
[60]
[PDF] TOOLS FOR VARROA MANAGEMENT - Honey Bee Health Coalition
Aug 1, 2022 · If post-treatment tests show that mite numbers remain above 3 percent after treatment, apply another control chemical or method without delay.
[61]
can Varroa explain part of the colony losses? - Apidologie
Most scientists agree that there is no single explanation for the extensive colony losses but that interactions between different stresses are involved.
[62]
Colony Collapse Disorder: A Descriptive Study | PLOS One
Elevated populations of varroa mites, Nosema spp., and honey bee tracheal mites (HBTM) are known to damage colonies and may contribute to CCD.
[63]
[PDF] First Detection and INITIAL DISTRIBUTION OF VARROA ...
The pest Varroa destructor was previously absent from Australia, but was detected on 22 June 2022 in New South Wales. Here, we describe the first 100 days ...
[64]
Colony Collapse Disorder | US EPA
Sep 11, 2025 · During the winter of 2006-2007, some beekeepers began to report unusually high losses of 30-90 percent of their hives. As many as 50 percent of ...Missing: surge spread
[65]
USDA Researchers Find Viruses from Miticide Resistant Parasitic ...
Jul 16, 2025 · In January 2025, commercial beekeepers began reporting severe losses in commercially managed operations. As losses unfolded, it was evident that ...Missing: distribution Oman Congo Pacific islands
[66]
U.S. Beekeeping Survey reveals highest honey bee colony losses ...
Jun 16, 2025 · The national survey estimated that 55.6% of managed honey bee colonies were lost in the country between April 2024 – April 2025.
[67]
The Varroa destructor mite is the greatest threat to US honeybees ...
Jan 22, 2020 · Managed honeybees play a crucial role in the nation's food production, contributing an estimated $15 billion to the U.S. economy each year by ...<|separator|>
[68]
New Data Confirm Catastrophic Honey Bee Colony Losses ...
“In January 2025, beekeepers across the country began reporting unexpected large-scale honey bee losses—we now know the largest ever recorded in the U.S.,” said ...Missing: destructor | Show results with:destructor
[69]
None
### Summary of Monitoring Methods, Thresholds, and Frequency from Varroa Management Guide
[70]
How to Quantify Varroa destructor in Honey Bee (Apis mellifera L ...
Feb 3, 2020 · This technique gives a more precise measurement of the Varroa infestation rate as the beekeeper can determine the exact number of bees and mites ...Missing: doubling | Show results with:doubling
[71]
Methods to Control Varroa Mites: An Integrated Pest Management Approach
### Summary of Monitoring Methods for Varroa destructor (Source: https://extension.psu.edu/methods-to-control-varroa-mites-an-integrated-pest-management-approach)
[72]
Varroa Mite Testing & Management - Bee Lab
If mite levels are above 1-2 mites per 100 bees, use Mite Away Quick Strips® or Formic Pro® as these products are safe to use with honey supers. VarroxSan ...Missing: threshold | Show results with:threshold
[73]
[PDF] An AI-Based Digital Scanner for Varroa destructor Detection in ...
Jan 14, 2025 · Results show that the system can consistently repeat measurements with high precision, with an error rate in detecting Varroa mites consistently ...<|control11|><|separator|>
[74]
Design of a Predictive Digital Twin System for Large-Scale Varroa ...
Overall, this work outlines a path toward real-time, data-driven Varroa management across apiary networks, from regional to cross-border scales. Keywords:.
[75]
[PDF] Varroa Resistance to Amitraz - Veto-pharma
Feb 28, 2025 · In general, acaricide treatments have an average effective- ness of about 95% for synthetic molecules and 90% for or- ganic molecules. A ...
[76]
Integrated resistance management for acaricide use on Varroa ...
Here, I examine acaricide resistance in varroa under the framework of Insecticide Resistance Management (IRM). I assess the potential of diverse IRM strategies.
[77]
Liquid and solid matrix formic acid treatment comparison against ...
Dec 13, 2023 · The efficacy of killing Varroa mites was found to be 96.0% ± 1.0 SE for the solid formic acid matrix and 95.4% ± 2.0 SE for the liquid formic ...
[78]
Resistance of Varroa destructor against Oxalic Acid Treatment—A ...
The review found no evidence of resistance to oxalic acid in Varroa destructor, despite some outliers, and further tests are needed.
[79]
First report of Varroa destructor resistance to pyrethroids in the UK
Aug 5, 2025 · PDF | Varroa destructor resistance to pyrethroids has been reported in mainland Europe since the early 1990's. V. destructor was first ...Missing: Czech Republic
[80]
Geographical distribution of pyrethroid resistance mutations in ...
Feb 24, 2024 · Point mutations in the sodium channel gene conferring tau-fluvalinate resistance in Varroa destructor. Pest Manag Sci. 2014;70(6):889–894 ...
[81]
EPA-registered Pesticide Products Approved for Use Against Varroa ...
Sep 26, 2025 · Vadescana works through a mechanism called RNA interference, which prevents the expression of a specific gene that the Varroa mite needs to ...
[82]
How to effectively manage residues from Varroa treatment
Jan 25, 2024 · Residues from several varroa treatments and pesticides can alter bee behavior, compromise foraging efficiency, and negatively impact colony productivity.
[83]
Combined treatment with amitraz and thymol to manage Varroa ...
May 28, 2024 · A combination of registered treatments (Apivar, amitraz-based; and Apiguard, thymol-based) could provide rapid and effective control of Varroa.
[84]
Efficacy of thymol nanoemulsion against Varroa destructor mites ...
Jul 31, 2024 · The highest total mean percentages of fallen Varroa were 70.45%, 67.59%, 60.08% and 51.49% after treatment with nanothymol 25 ppm, thymol 100 ...<|control11|><|separator|>
[85]
[PDF] Integrated Hive Mangement for Colorado Beekeepers
Mite levels can be reduced as much as 25% by using screened bottom boards. Specially made drone comb foundation or removal of excess drone cells can reduce mite ...Missing: efficacy | Show results with:efficacy
[86]
[PDF] Drone brood removal for Varroa control - National Bee Unit
This has the potential to reduce the mite population by approximately 90%, or by as much as 99% if a second follow up drone brood comb is used3. Broodless ...Missing: percentage scientific
[87]
[PDF] National Bee Unit - Queen trapping
Trapping the queen in a cage can prevent her from laying, which induces a brood break in a colony and reduces the mite population in the colony by slowing ...Missing: drop | Show results with:drop
[88]
The efficacy of small cell foundation as a varroa mite ... - PubMed
To summarize our results, we found that the use of small cell foundation did not significantly affect cm(2) total brood, total mites per colony, mites per brood ...
[89]
What beekeeping best practices help reduce the threat of Varroa?
Aug 8, 2025 · Preventing drift, such as the use of different colored boxes, keeping hives at least three to six feet apart, staggering hive placement, place ...
[90]
Distance between honey bee Apis mellifera colonies regulates ... - NIH
May 2, 2016 · Abstract. Inter-colony distance of Apis mellifera significantly affects colony numbers of the parasitic mite Varroa destructor.
[91]
An investigation of the efficacy of hygienic behavior of various honey ...
The smaller width of cells size combs reduces the Varroa mite reproduction behavior compared to larger cell size combs. More infestation of Varroa mite ...
[92]
Breeding Varroa-Resistant Bees: The Long Road - Project Apis m.
May 16, 2025 · One way bees successfully resist the mite is by expressing a high level of Varroa Sensitive Hygiene (VSH) behavior, which results in the bees ...Missing: Minnesota efficacy
[93]
Results of four generations of selection for Varroa Sensitive hygienic ...
A steady increase in mean SMR scores was observed, rising from 22.1% in 2021 to 41.0% in 2024, along with greater phenotypic variability and the appearance of ...
[94]
(PDF) RNA interference as a next‐generation control method for ...
May 27, 2024 · RNA interference as a next‐generation control method for suppressing Varroa destructor reproduction in honey bee (Apis mellifera) hives.
[95]
First evidence of the effectiveness of a field application of RNAi ...
Jan 27, 2025 · Our findings demonstrate the feasibility and effectiveness of RNAi technology in reducing Varroa mite infestations under natural rearing conditions.
[96]
Identification and CRISPR-Cas9 validation of a novel β-adrenergic ...
Identification and CRISPR-Cas9 validation of a novel β-adrenergic-like octopamine receptor mutation associated with amitraz resistance in Varroa destructor.
[97]
Directed evolution of Metarhizium fungus improves its biocontrol ...
May 19, 2021 · Field tests with Metarhizium showed the fungus was capable of controlling mites, sometimes with results comparable to the commonly used chemical ...
[98]
Risk assessment and predation potential of Stratiolaelaps scimitus ...
Dec 7, 2018 · Our study aimed to investigate the potential of the predatory mite Stratiolaelaps scimitus to control Varroa infestations in honey bees.
[99]
GreenLight Biosciences Launches Norroa, the First RNA-Based ...
Sep 25, 2025 · Rigorous, replicated field trials conducted across multiple U.S. regions demonstrated extended mite control of up to 18 weeks, resulting in ...Missing: varroacides | Show results with:varroacides
[100]
Selective Breeding for Low and High Varroa destructor Growth in ...
Dec 4, 2020 · The results of this study thus far indicate that selection for LVG may result in colonies with lower Varroa infestation rates, lower prevalence, and levels of ...
[101]
Breeding for resistance to Varroa destructor in Europe - Apidologie
The rich variety of native honeybee subspecies and ecotypes in Europe offers a good genetic resource for selection towards Varroa resistance.