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Asymmetric cell division

Asymmetric cell division (ACD) is a conserved cellular process in which a progenitor or stem cell divides to generate two daughter cells with distinct fates—one typically retaining stem-like properties for self-renewal and the other committing to differentiation—through the unequal inheritance of cellular components, including proteins, mRNAs, organelles, and epigenetic marks.^[1] This contrasts with symmetric cell division, which produces two identical daughter cells, and ACD plays a pivotal role in generating cellular diversity during embryonic development, maintaining tissue homeostasis in adults, and regulating stem cell populations across organisms from prokaryotes to eukaryotes, including yeast to mammals.^[2] The importance of ACD lies in its ability to balance self-renewal and differentiation, preventing stem cell exhaustion or uncontrolled proliferation that could lead to tissue degeneration or cancer.^[3] In model organisms, ACD has been extensively studied: in Drosophila melanogaster neuroblasts, fate determinants like Prospero and Numb localize basally to direct one daughter toward neuronal differentiation while the other self-renews; in Caenorhabditis elegans embryos, PAR polarity proteins establish an apical-basal axis to segregate determinants during the first zygotic division; and in mammalian hematopoietic stem cells, asymmetric segregation of proteins such as Numb influences lineage commitment.^[1] Key mechanisms underlying ACD include the establishment of cell polarity via conserved complexes (e.g., PAR/aPKC), asymmetric localization of fate determinants along the polarity axis, precise orientation of the mitotic spindle through cytoskeletal elements like actin and microtubules, and checkpoint controls to ensure faithful segregation during cytokinesis.^[2] These processes are influenced by both intrinsic factors, such as centrosome asymmetry, and extrinsic cues from the stem cell niche, like Wnt or Notch signaling, highlighting ACD's integration of environmental and internal signals to drive binary cell fate decisions.^[3]

Introduction and Fundamentals

Definition and Types

Asymmetric cell division is a fundamental cellular process in which a progenitor cell divides to produce two daughter cells that differ in developmental potential, size, or molecular composition, thereby generating cellular diversity from a single precursor.^[4] This contrasts with symmetric cell division, which yields two identical daughter cells with equivalent fates and characteristics.^[1] The asymmetry arises through the unequal distribution of cellular components, such as proteins, RNAs, or organelles, which dictates distinct trajectories for the daughters—one often retaining stem-like properties while the other differentiates.^[5] Asymmetric cell division can be classified into two primary types based on the source of asymmetry: intrinsic and extrinsic. Intrinsic asymmetric division is cell-autonomous, driven by internal mechanisms that establish polarity within the cell, leading to the unequal segregation of fate determinants during mitosis.^[6] In this type, the cell's own molecular machinery polarizes components independently of external inputs, ensuring reproducible asymmetry.^[7] Extrinsic asymmetric division, conversely, depends on environmental cues from the surrounding microenvironment or neighboring cells, such as signaling gradients or niche interactions, which orient the division plane and influence daughter cell fates.^[8] These types are not mutually exclusive and can interplay to fine-tune outcomes in various biological contexts.^[9] The concept of asymmetric cell division was first observed in the early 20th century through embryological studies, notably by Edwin G. Conklin in 1905, who described unequal cytoplasmic partitioning in ascidian embryos.^[5] Modern molecular understanding emerged in the 1980s and 1990s, propelled by genetic analyses in model organisms that revealed regulatory pathways governing asymmetry.^[4] Central to this process are key concepts including the unequal partitioning of fate determinants—molecules that bias cell fate toward differentiation or self-renewal; the orientation of the mitotic spindle, which aligns the division axis to segregate components asymmetrically; and cytokinesis asymmetry, where the cleavage furrow forms unevenly to produce daughters of differing sizes.^[10] These elements collectively ensure precise control over cellular heterogeneity.^[11]

Biological Significance

Asymmetric cell division (ACD) represents an evolutionarily conserved process fundamental to the generation of cellular diversity across biological kingdoms. Observed in prokaryotes such as bacteria and extending to eukaryotes ranging from unicellular yeasts to complex multicellular animals like vertebrates, ACD enables the production of daughter cells with distinct fates from a single progenitor, thereby optimizing resource allocation and adaptation in diverse environments.^[1] This conservation underscores its ancient origins, with core principles of polarity establishment and unequal inheritance preserved over billions of years of evolution.^[12] In developmental biology, ACD plays a pivotal role in establishing embryonic polarity and rapidly diversifying cell lineages, allowing a single zygote to give rise to hundreds of specialized cell types within a limited number of divisions. By partitioning fate determinants unequally, it promotes efficient tissue formation and pattern establishment, ensuring the precise orchestration of morphogenesis without excessive proliferation.^[5] In adult tissues, ACD contributes to homeostasis by regulating the balance between cell renewal and replacement, thereby sustaining organ function over the organism's lifespan.^[12] Particularly in stem cell populations, ACD is essential for maintaining long-term reservoirs of undifferentiated cells while simultaneously producing committed progeny for tissue repair and growth. This asymmetry prevents the exhaustion of stem cell pools by segregating aging factors, such as damaged organelles or proteins, into differentiating daughters, thus enhancing cellular quality control and longevity.^[3] Overall, these functions confer broader organismal robustness, enabling resilience to environmental stresses, injuries, and physiological demands through reliable fate decisions and adaptive responses.^[1]

Mechanisms of Asymmetric Cell Division

Intrinsic Mechanisms

Intrinsic mechanisms of asymmetric cell division rely on cell-autonomous processes that establish and maintain polarity, orient the mitotic spindle, and ensure unequal partitioning of cellular components to generate daughter cells with distinct fates. Polarity establishment typically involves the formation of an apical-basal or anterior-posterior axis through the localized assembly of protein complexes, such as the PAR complex comprising PAR-3, PAR-6, and atypical protein kinase C (aPKC), which segregate into distinct cortical domains to define cellular asymmetry.^[1] This polarization creates a scaffold for subsequent asymmetric events, enabling the differential distribution of fate determinants without reliance on external cues.^[13] Mitotic spindle alignment is a critical intrinsic step where the spindle orients perpendicular or parallel to the polarity axis, ensuring unequal segregation of determinants. This process involves microtubule-associated proteins, such as those in the Pins/Gαi complex, which anchor astral microtubules to the polarized cortex, and centrosome asymmetry, where the older mother centrosome and younger daughter centrosome exhibit distinct microtubule-organizing capacities to bias spindle positioning.^[1] Centrosome asymmetry arises from inherent differences in centriole age and associated proteins like Plk1, which maintain active microtubule nucleation at one pole, thereby directing the spindle toward the apical domain.^[13] Unequal segregation during cytokinesis partitions organelles and molecules asymmetrically between daughters. For instance, centrosomes are often inherited unequally, with the daughter centrosome directed to the self-renewing cell and the mother centrosome to the differentiating cell, influencing proliferative potential through differences in microtubule dynamics.^[1] Molecular determinants, including transcription factors, are localized to one pole via adaptor proteins and transported along microtubules, ensuring their enrichment in the basal daughter.^[13] Size asymmetry, another outcome, stems from membrane reservoir dynamics, where polarized reservoirs of membrane—often enriched in endosomes or invaginations—are preferentially consumed by one daughter, driving unequal surface expansion via actomyosin contractility and cortical flow.^[14] Emerging intrinsic mechanisms include asymmetric segregation of additional organelles, such as mitochondria, which are unequally partitioned during divisions in model systems to influence metabolic fates in daughters (as of August 2025), and lysosomes, whose biased inheritance modulates Notch signaling in human neural stem cells.^[15]^[16] To quantify cytokinesis bias in size asymmetry, researchers employ an asymmetry index defined as (V_1 - V_2) / (V_1 + V_2), where V_1 and V_2 represent the volumes of the two daughter cells; values approaching 1 indicate strong bias toward one cell, while 0 denotes symmetry.^[17] This metric highlights how intrinsic imbalances in membrane allocation and spindle geometry contribute to functional diversity in daughter cells.

Extrinsic Mechanisms

Extrinsic mechanisms in asymmetric cell division involve environmental cues from the surrounding tissue or niche that impose asymmetry on the dividing cell, often by influencing spindle orientation, fate determinant localization, or cytokinesis plane. These non-cell-autonomous signals contrast with intrinsic factors by relying on interactions with neighboring cells or the extracellular environment to bias daughter cell fates. Such cues are crucial in stem cell niches, where they help maintain tissue homeostasis by promoting self-renewal in one daughter while driving differentiation in the other.^[1] Niche signaling, particularly through contact-dependent pathways like Delta-Notch interactions, plays a pivotal role in biasing asymmetric outcomes. In this system, the ligand Delta on one cell activates Notch receptor on an adjacent cell, leading to differential signaling that segregates fate determinants during division. For instance, in Drosophila sensory organ precursor cells, asymmetric Delta-Notch activation results in one daughter inheriting higher Notch activity, promoting a specific fate while the other adopts an alternative path. This contact-mediated signaling ensures precise fate specification within crowded niches.^[13]^[18] Soluble factors, such as morphogen gradients of Wnt or BMP, further modulate asymmetry by creating spatial biases that affect spindle positioning or protein localization. Wnt signaling, for example, orients the mitotic spindle in C. elegans EMS cells via a neighboring cell-derived ligand, ensuring proper determinant segregation and embryonic patterning. Similarly, BMP gradients influence spindle asymmetry in mammalian neural progenitors, where steeper gradients correlate with enhanced proliferative bias in daughter cells, reducing neurogenic output. These diffusible cues establish concentration-dependent thresholds that dictate division outcomes across tissues.^[19] Mechanical forces from the extracellular matrix (ECM) also contribute by altering the cytokinesis plane through adhesion asymmetry or shear stress. In epithelial tissues, uneven ECM attachments generate cortical tension gradients that rotate the spindle and bias cleavage furrow positioning, as observed in mechanically constrained cells where adhesion hotspots promote unequal division. Such forces integrate with soluble signals to fine-tune asymmetry in dynamic environments like developing organs.^[20]^[21] Extrinsic signals often interface with intrinsic pathways by inducing localized polarization, such as through PIP3 accumulation at the plasma membrane. For example, external cues like integrin-mediated ECM contacts activate PI3K, leading to PIP3 enrichment at one pole, which in turn orients the spindle without relying solely on internal polarity regulators. This integration allows environmental inputs to override or amplify cell-autonomous biases.^[22] Recent studies highlight roles for long non-coding RNAs (lncRNAs) in extrinsic regulation, such as miR-146a-mediated lncRNA effects promoting asymmetry in breast cancer stem cell niches via Let-7c modulation (as of 2024).^[23] Quantification of these extrinsic effects has advanced through live-cell imaging techniques that track signaling dynamics in real time. In Drosophila neuroblasts, fluorescence microscopy coupled with the MS2 system visualizes mRNA gradients, revealing how steepness in morphogen profiles (e.g., Wnt) directly impacts division asymmetry, with quantitative metrics showing 20-30% variation in determinant localization tied to gradient slope. These approaches highlight the spatiotemporal precision required for robust asymmetric divisions.^[24]

Asymmetric Division in Model Organisms

In C. elegans

Asymmetric cell division in the nematode Caenorhabditis elegans begins with the first cleavage of the zygote, which produces two unequal daughter cells: the larger anterior AB blastomere, destined to generate a variety of multipotent somatic lineages including neurons, hypodermis, and pharynx, and the smaller posterior P1 blastomere, which serves as the precursor for germline cells and additional somatic tissues.^[1] This division establishes the initial anterior-posterior axis through both unequal cytokinesis, resulting in disparate cell sizes, and the asymmetric segregation of cytoplasmic determinants that dictate distinct cell fates. The AB cell adopts a faster cell cycle and contributes to anterior structures, while P1 undergoes slower divisions and retains germline potential. Polarity establishment is mediated by the conserved partitioning defective (PAR) proteins, which localize asymmetrically to the cortex shortly after fertilization in response to sperm-induced cues. The anterior cortex is enriched with PAR-3, PAR-6, and atypical protein kinase C (PKC-3), forming a complex that excludes posterior factors, while the posterior cortex recruits PAR-1 and PAR-2 kinases, which mutually antagonize the anterior PAR complex to maintain distinct domains. These PAR proteins regulate the localization of fate determinants, including the RNA-binding protein PIE-1 and maternal mRNAs, ensuring their enrichment in P1. Notably, P granules—electron-dense ribonucleoprotein complexes associated with germline specification—are initially uniform but become asymmetrically segregated to the posterior cortex of the zygote and inherited exclusively by P1, a process dependent on PAR-2 for cortical anchoring.^[25] During mitosis, asymmetric spindle positioning and cytokinesis further reinforce this polarity. The mitotic spindle forms centrally but shifts posteriorly due to stronger astral microtubule-pulling forces generated by a posterior cortical gradient of force generators, involving heterotrimeric G proteins and regulators like GOA-1/GPA-16, which are modulated by the PAR domains. This skewing, combined with actomyosin contractility differences across the cortex, ensures the cleavage furrow forms off-center, producing the size disparity between AB and P1 and aligning the division with the polarity axis. Cortical flow driven by non-muscle myosin II (NMY-2), enriched anteriorly by the PAR-3/PAR-6/PKC-3 complex, also contributes to determinant segregation and spindle orientation.^[26] Subsequent divisions build on this foundation, as seen in the second cleavage of P1 into the anterior EMS blastomere (which produces mesoderm and endoderm) and posterior P2 (germline precursor). The EMS cell then undergoes another asymmetric division, yielding the anterior MS daughter (mesodermal fates like body muscle and pharynx) and posterior E daughter (intestinal endoderm), guided by extrinsic GLP-1/Notch signaling from the neighboring P2 cell. This interaction polarizes EMS via localized activation of the GLP-1 receptor on its anterior side, promoting MS specification while repressing endodermal fates posteriorly.^[1] Experimental perturbations underscore the essentiality of these mechanisms; mutations in par genes, such as par-1, par-2, or par-3, disrupt polarity, leading to equal-sized daughter cells, randomization of determinant segregation, and complete embryonic lethality due to failure in axis formation and cell fate specification. Similarly, RNA interference (RNAi) knockdowns of PAR components recapitulate these phenotypes, confirming their roles in vivo and highlighting the precision required for viable development.^[25]

In Drosophila Neurogenesis

In Drosophila embryonic neurogenesis, neural stem cells known as neuroblasts form through delamination from the neuroectoderm, a process that inherits and establishes apical-basal polarity.^[27] This polarity is initiated in the prospective neuroblasts within the neuroectoderm, where apical localization of proteins such as Bazooka/Par-3, Par-6, and atypical protein kinase C (aPKC) occurs prior to delamination.^[28] Upon delamination, the neuroblasts round up and further polarize along the apical-basal axis, with the adaptor protein Inscuteable recruiting the G-protein signaling regulator Pins (Partner of Inscuteable) to the apical cortex, thereby linking polarity to spindle orientation.^[1] Each neuroblast undergoes asymmetric cell division, generating a self-renewing apical neuroblast and a smaller basal ganglion mother cell (GMC).^[29] The GMC subsequently divides symmetrically once to produce two differentiated cells, typically neurons or glia, thereby amplifying the neural lineage.^[27] This asymmetry is achieved through the basal segregation of cell fate determinants during mitosis, including the adaptor protein Miranda, which binds and localizes the transcription factor Prospero and the Notch inhibitor Numb to the basal cortex.^[30] Prospero enters the GMC nucleus to promote neuronal differentiation and suppress neuroblast self-renewal, while Numb inhibits Notch signaling in the GMC, preventing it from adopting a neuroblast-like fate.^[31] Spindle orientation is critical for proper asymmetric partitioning and is regulated by interactions between apical astral microtubules and the cell cortex. During prometaphase, the mitotic spindle aligns perpendicular to the neuroectoderm surface, with astral microtubules emanating from the apical centrosome contacting Pins and Gαi at the apical cortex to anchor and orient the spindle along the polarity axis.^[1] This microtubule-dependent mechanism ensures that basal determinants are inherited by the GMC. Mutations disrupting polarity components lead to symmetric divisions and neural overproliferation. In pins mutants, neuroblasts fail to orient their spindles correctly, resulting in both daughters inheriting self-renewal factors and producing excess neuroblasts at the expense of differentiated neurons. Similarly, lethal giant larvae (lgl) mutants exhibit basal mislocalization of aPKC, causing symmetric self-renewal divisions; lgl pins double mutants exacerbate this, filling the brain with undifferentiated neuroblasts and causing massive overgrowth.^[32]

In Spiralian Animals

Spiral cleavage is a hallmark of early embryonic development in spiralian animals, including mollusks and annelids, characterized by a series of dextral or sinistral spiraling asymmetric cell divisions that produce blastomeres of unequal size and fate.^[33] These divisions begin after the first two symmetric cleavages, which generate four equal blastomeres arranged in a quadrant pattern (A, B, C, D), followed by oblique, rotational divisions that create smaller micromeres toward the animal pole and larger macromeres toward the vegetal pole.^[34] This pattern establishes initial polarity and contributes to the invariant cell lineage typical of spiralian embryogenesis, contrasting with the more regulative bilateral cleavage seen in deuterostomes.^[35] The mechanisms underlying these asymmetric divisions involve unequal inheritance of cellular components, including centrosomes and cytoplasmic determinants. Unequal centrosome inheritance arises from transient disassembly of one spindle pole aster during metaphase, leading to asymmetric microtubule asters that bias spindle orientation and cytokinesis toward one side, as observed in the annelid Helobdella robusta.^[36] Microtubule aster asymmetry further drives this process by generating unequal forces on the cell cortex, promoting the formation of unequal daughter cells without centrosome loss.^[33] Cytoplasmic determinants, such as localized maternal mRNAs and proteins, are segregated during ooplasmic movements post-fertilization, establishing animal-vegetal polarity that influences subsequent divisions; for left-right asymmetry, nodal signaling plays a key role by promoting asymmetric gene expression in mesodermal cells, as seen in gastropod mollusks like Lottia gigantea.^[37] Representative examples illustrate these processes in specific spiralian species. In the mollusk Ilyanassa obsoleta, the polar lobe—a transient vegetal protrusion containing determinants—is inherited exclusively by the D quadrant macromeres during the first two cleavages, enabling the 4d mesentoblast cell to specify mesodermal and endodermal fates, including mesentoblasts that give rise to the mesoderm.^[38] Similarly, in the annelid Tubifex tubifex, teloplasm (yolk-free pole plasm rich in germ determinants) undergoes asymmetric segregation during the third cleavage, localizing primarily to the 4d micromere of the D quadrant, which serves as a stem cell precursor for mesodermal teloblasts.^[39] These unequal partitions ensure that the D lineage receives specialized cytoplasm essential for organogenesis. Evolutionarily, asymmetric cell division via spiral cleavage is conserved across lophotrochozoans, a major clade of spiralians encompassing mollusks, annelids, and brachiopods, reflecting an ancient protostome innovation that predates the divergence of these phyla.^[35] This conservation contrasts with the bilateral cleavage patterns in ecdysozoans and deuterostomes, where asymmetry emerges later through different mechanisms like ciliary flows, highlighting spiral cleavage's role in early symmetry breaking within lophotrochozoans.^[37] Time-lapse imaging studies have revealed the dynamics of cytokinesis furrow bias in spiralian embryos, driven by actomyosin contractility. In scallop embryos (Patinopecten yessoensis), live imaging shows enrichment of actin and phosphorylated myosin II at the polar lobe constriction site during early cleavages, creating a biased furrow that ingresses perpendicular to the main cleavage plane and segregates determinants unequally.^[40] This actomyosin-mediated asymmetry, coupled with astral microtubule pulling forces, ensures precise partitioning of cytoplasm, as visualized through super-resolution microscopy.^[40]

Asymmetric Division in Stem Cells

In Neural Stem Cells

In the mammalian cortex, radial glial cells (RGCs) serve as neural progenitors and primarily divide asymmetrically in an oblique manner during mouse neocortical development, producing one RGC and one neuron or intermediate progenitor. This oblique division orientation, distinct from the vertical divisions that expand the progenitor pool, relies on the cortical localization of NuMA and LGN to guide mitotic spindle positioning perpendicular to the ventricular surface. Disruption of this NuMA/LGN complex leads to misoriented spindles and altered fate outcomes, highlighting its role in balancing neurogenesis.^[41]^[42] In vertebrates, the Par3/Par6/aPKC complex establishes apical-basal polarity in RGCs, with Par3 localizing to the apical domain to regulate spindle orientation and Notch-mediated fate decisions, ensuring one daughter maintains stem-like properties.^[13]^[43] Extrinsic niche interactions further bias asymmetric divisions in the ventricular zone. Beta-catenin signaling from the ventricular surface provides an extrinsic cue that promotes RGC proliferation and self-renewal, with its activation in contact with the niche influencing division outcomes through Wnt pathway modulation. This signaling integrates with intrinsic polarity to prevent symmetric neurogenic divisions that would deplete the progenitor pool prematurely.^[44]^[45] Dysregulation of asymmetric divisions in neural stem cells can shift toward symmetric proliferative modes, leading to hyperplasia and overproduction of progenitors, or symmetric differentiative modes, causing premature depletion of the stem cell pool. Such imbalances disrupt cortical layering and neuron numbers, with implications for neurodevelopmental pathologies.^[46]^[47]

In Mammalian Somatic Stem Cells

In the intestinal crypts of the mammalian small intestine, Lgr5+ crypt base columnar stem cells maintain epithelial homeostasis through oriented asymmetric cell divisions aligned along the crypt-villus axis. These divisions typically generate one daughter cell that retains stem cell identity at the crypt base, in close proximity to Paneth cells, and another that adopts a transit-amplifying fate and migrates upward toward the villus tip. This orientation is regulated by the stem cell niche, where Paneth cells secrete Wnt ligands to sustain self-renewal in basal-positioned daughters while Notch signaling promotes differentiation in the displaced progeny.^[48]^[49] Hematopoietic stem cells (HSCs) in the mouse bone marrow exhibit asymmetric divisions characterized by the unequal inheritance of fate determinants, such as the adaptor protein Numb, which localizes to one pole of the mitotic spindle and inhibits Notch signaling asymmetrically to favor self-renewal in one daughter and differentiation in the other. This process is evidenced by unequal retention of DNA labels, where HSCs preferentially segregate older template DNA strands to the self-renewing daughter, minimizing replication errors and supporting long-term repopulation potential. Flow cytometry studies of marker distribution, including Numb and DNA labels, indicate that approximately 30-50% of HSC divisions display such asymmetry, balancing the stem cell pool with progenitor output.^[50]^[51]^[52] In the skin, bulge stem cells within hair follicles contribute to tissue renewal via a combination of planar and perpendicular divisions, where planar orientations often yield symmetric outcomes to expand the stem cell compartment during quiescence, and perpendicular divisions to the basement membrane promote asymmetric fates for homeostasis and hair cycle progression. Similar dynamics occur in skeletal muscle satellite cells, which undergo oriented asymmetric divisions to produce one self-renewing stem cell and one committed myogenic progenitor. These processes are modulated by niche-derived Wnt and Notch gradients, which establish polarity and influence spindle alignment. Recent investigations into HSC membrane dynamics have revealed that heterotypic interactions with osteoblasts drive asymmetric lysosome inheritance during divisions, altering membrane composition and metabolic states in daughter cells to reinforce fate decisions.^[53]^[54]

Role in Disease

In Cancer

In cancers, disruptions to asymmetric cell division often lead to a shift toward symmetric proliferative divisions, allowing progenitor cells to expand the pool of tumor-initiating cells rather than producing differentiated progeny. For instance, in brain tumors such as gliomas, neural progenitor cells that normally undergo asymmetric divisions to balance self-renewal and differentiation instead favor symmetric divisions, contributing to tumor initiation and growth.^[55] This shift is exemplified in CD133-positive glioma stem cells, where symmetric division maintains stem-like properties and promotes tumor propagation.^[56] Key molecular pathways underscore this dysregulation. Loss of the adaptor protein Numb, which inhibits Notch signaling during asymmetric division, results in elevated Notch activity that favors self-renewal and symmetric proliferative outcomes in cancer cells.^[57] In Drosophila models, mutations in the tumor suppressor lethal giant larvae (lgl) disrupt cell polarity and asymmetric segregation of fate determinants, leading to uncontrolled proliferation that mimics epithelial cancers, including intestinal types, and has been linked to human colorectal cancer progression.^[58] These defects highlight how polarity regulators prevent tumorigenesis by enforcing asymmetric outcomes. The asymmetric division hypothesis posits that cancer stem cells (CSCs) use this process to generate heterogeneity, with one daughter retaining stemness and the other differentiating, thereby sustaining tumor maintenance and conferring therapy resistance. A critical mechanism involves unequal partitioning of drug efflux pumps, such as ABC transporters, during division; in glioblastoma stem cells, this asymmetry allows one daughter to acquire enhanced efflux capacity, evading chemotherapeutic agents like temozolomide.^[59] In human cancers, Wnt pathway dysregulation in colorectal CSCs promotes symmetric self-renewal by altering β-catenin distribution, expanding the CSC population and driving metastasis.^[60] Similarly, BRCA1 deficiency impairs spindle orientation and cortical asymmetry of dynein complexes, leading to loss of asymmetric division and increased ploidy instability in breast and ovarian cancers.^[61] Therapeutic strategies target these disruptions to restore polarity and asymmetric outcomes. Aurora kinase inhibitors, such as alisertib, disrupt the phosphorylation of polarity complexes like Par3/aPKC, which normally regulate Numb localization; in preclinical models, this forces symmetric divisions in CSCs, depleting the stem cell pool and sensitizing tumors to therapy.^[62] Recent studies on glioma have identified polarized membrane reservoirs in neural stem cells that drive asymmetric expansion during division; targeting these reservoirs, as explored in 2023 research, could prevent symmetric shifts in glioma CSCs by modulating membrane dynamics and polarity.^[14] As of 2025, emerging evidence links ACD to metabolic heterogeneity in ALDH1-positive CSCs and epigenetic regulation via long non-coding RNAs (LncRNAs), further contributing to tumor diversity and resistance.^[63]^[64]

In Developmental Disorders

Defects in asymmetric cell division contribute to various developmental disorders by disrupting embryonic patterning, neural tube formation, and stem cell maintenance. In ciliopathies such as Bardet-Biedl syndrome (BBS), mutations in BBS genes impair primary cilia function, which is critical for generating nodal flow that establishes left-right asymmetry during gastrulation.^[65] This directional flow, produced by motile cilia in the embryonic node, breaks bilateral symmetry to specify organ positioning; BBS proteins, as components of the BBSome complex, facilitate intraflagellar transport necessary for proper ciliary motility and signaling.^[66] Disruption of this process leads to situs inversus and other congenital malformations in BBS patients, as unequal distribution of signaling molecules like Nodal fails to create asymmetric gene expression domains.^[67] Furthermore, BBS proteins influence planar cell polarity (PCP) pathways, which regulate oriented asymmetric divisions in epithelial tissues, exacerbating patterning failures.^[67] Neural tube defects, including spina bifida, arise from imbalances in symmetric versus asymmetric divisions of neural progenitor cells, leading to premature depletion of the progenitor pool and defective neural tube closure. In mouse models, disruption of PCP components like Vangl2 causes misoriented mitotic spindles in neuroepithelial cells, shifting divisions from symmetric proliferative modes to asymmetric differentiative ones too early, which impairs convergent extension and elevates the neural folds.^[68] This results in open neural tube phenotypes resembling human spina bifida, where failure to maintain progenitor expansion reduces cortical layering and spinal cord integrity.^[68] Such defects highlight how intrinsic spindle positioning errors, often tied to polarity regulators, propagate to broader morphogenetic failures during neurulation. In progeria syndromes like Hutchinson-Gilford progeria syndrome (HGPS), LMNA mutations lead to stem cell exhaustion in hematopoietic stem cells (HSCs), manifesting as premature aging phenotypes such as bone marrow failure. The LMNA mutation in HGPS disrupts nuclear lamina integrity, impairing mechanotransduction and depleting the stem cell reservoir.^[69] This shift reduces long-term repopulation capacity, contributing to systemic aging features like cardiovascular disease and frailty observed in patients.^[69] Model organisms provide key insights into these human disorders; for instance, PAR protein mutants in C. elegans exhibit polarity defects that parallel human ciliopathies, with failed asymmetric segregation of fate determinants leading to embryonic inviability akin to polarity disruptions in BBS. In mice, mutations in ASPM, a microcephaly-associated gene, cause spindle misorientation in neural progenitors, promoting excessive asymmetric fates and resulting in microcephaly through reduced progenitor proliferation.^[70] These models underscore conserved roles of polarity machinery in preventing congenital brain malformations. Diagnostic advances include time-lapse imaging of early cleavage asymmetry in IVF embryos, which identifies abnormal division patterns predictive of developmental risks. Embryos displaying irregular blastomere symmetry or delayed cleavages show higher rates of aneuploidy and arrest, correlating with increased likelihood of congenital anomalies like neural tube defects.^[71] Such non-invasive assessments enable selection of viable embryos, potentially mitigating risks of implantation failure or birth defects.^[72]

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From spiral cleavage to bilateral symmetry: the developmental cell ...
Oct 22, 2019 · The subsequent cleavages are asymmetrical, generating quartets of smaller micromeres towards the animal pole and quartets of bigger macromeres ...
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Animal left–right asymmetry - ScienceDirect.com
Apr 2, 2018 · Mollusks and annelids, and lophotrochozoans in general, undergo spiral cleavage divisions ... The evolution and conservation of left-right ...
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Asymmetrization of first cleavage by transient disassembly of one ...
Both centrosomes are retained throughout mitosis; the unequal cleavage is thought to result from asymmetric forces acting on the two asters, as a result of a ...
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Embryonic chirality and the evolution of spiralian left–right ... - Journals
Dec 19, 2016 · With the third round of zygotic divisions, a typical spiral-cleaving embryo becomes eight cells. These divisions are asymmetric and occur in the ...
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Cell fate maps in the Ilyanassa obsoleta embryo beyond ... - PubMed
Sep 15, 1997 · In the gastropod Ilyanassa obsoleta, early development is mediated by the polar lobe, which shunts determinants to the D lineage during the ...
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Asymmetric Segregation and Polarized Redistribution of Pole Plasm ...
The first step is asymmetric segregation which results in bipolar localization of pole plasm masses in the D-cell of the 4-cell embryo. The spatial ...Missing: division | Show results with:division
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Cytoskeletal polarization and cytokinetic signaling drives polar lobe ...
Aug 31, 2019 · Image Acquisition and Analysis. For timelapse imaging of scallop embryos, embryos were cultured at 12°C on a Brook cooling microscope stage ...
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Oblique Radial Glial Divisions in the Developing Mouse Neocortex ...
Mar 9, 2011 · Therefore, the mechanisms underlying asymmetric division of radial glia cells are fundamental to brain development. Studies of invertebrate ...Missing: NuMA seminal
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A lateral belt of cortical LGN and NuMA guides mitotic spindle ...
Knockdown or mislocalization of LGN complex components disrupts the stereotypic biphasic spindle movements regulating planar cell division and neuroepithel.Missing: seminal | Show results with:seminal
[39]
Asymmetric Distribution of EGFR Receptor during Mitosis Generates ...
While in Drosophila asymmetric neural cell divisions are largely dependent on Numb, and all known Numb functions act via the Notch pathway, in vertebrates this ...Oligodendrocytes And... · Egfr Asymmetry During... · The Role Of Egfr Asymmetry...
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Cell-Autonomous β-Catenin Signaling Regulates Cortical Precursor ...
These results show that β-catenin-mediated transcriptional activation functions in the decision of cortical ventricular zone precursors to proliferate or ...
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Wnt/β-catenin signaling in cerebral cortical development - PMC
Radial glia cells can divide asymmetrically in a stem-cell mode to generate one radial glial cell and one intermediate progenitor, which then divides ...
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Regulation of Asymmetric Cell Division in Mammalian Neural Stem ...
Aug 10, 2025 · PDF | Stem and progenitor cells are characterized by their abilities to self-renew and produce differentiated progeny.
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asymmetric cell division and its implications for stem cells and cancer
Sperm entry triggers a series of events that result in a subdivision of the cell cortex into an anterior and a posterior domain (Cowan and Hyman 2004a). This ...
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Asymmetric cell division-dominant neutral drift model for normal ...
Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells. Cell 143: 134–144, 2010. doi: 10.1016/j.cell ...Missing: Lgr5+ | Show results with:Lgr5+
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Molecular mechanisms of asymmetric divisions in mammary stem cells
Nov 21, 2016 · Stem cells have the remarkable ability to undergo proliferative symmetric divisions and self‐renewing asymmetric divisions.
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Asymmetric cell division of hematopoietic stem cells - Frontiers
Here we provide a historical perspective and discuss the recent advances in our understanding of ACD and the role of lysosomes in HSC function.
[47]
Biased DNA Segregation During Stem Cell Division
May 25, 2012 · Stem cell division is characterized by asymmetric segregation of chromatids so that one daughter cell contains only the old intact DNA and the other carries ...
[48]
Asymmetric cell division within the human hematopoietic stem and ...
Thus, it remains an open question as to whether HSCs/HPCs have the capability to divide asymmetrically, or whether the differences that have been observed are ...
[49]
Stem cell dynamics in mouse hair follicles - NIH
Here we provide data suggesting that in hair follicle stem cells the self-renewing divisions within the niche (bulge) are symmetric with respect to localization ...
[50]
Distribution of CD133 reveals glioma stem cells self-renew through ...
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ASYMMETRIC CELL DIVISION: IMPLICATIONS FOR GLIOMA ...
Radial glial cells are able to divide either symmetrically or asymmetrically (Figure 1) and have been shown to undergo proliferative symmetrical cell divisions ...
[52]
Drosophila Lethal Giant Larvae Neoplastic Mutant as a Genetic Tool ...
The Par complex directs asymmetric cell division by ... Drosophila tumour suppressor gene lgl, contributes to progression of colorectal cancer.Missing: intestinal | Show results with:intestinal
[53]
Asymmetric cell division promotes therapeutic resistance in ...
Asymmetric cell division promotes therapeutic resistance in glioblastoma stem cells ... drug efflux pumps, enhanced DNA repair capacity, slow proliferation ...
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The Wnt/β-catenin pathway regulates growth and maintenance of ...
Aug 6, 2010 · CSCs share all the fundamental traits of stem cells-self renewal by asymmetric division, reduced proliferation and differentiation and ...The Wnt/β-Catenin Pathway... · Discussion · β-Catenin Sirna...
[55]
BRCA1 controls the cell division axis and governs ploidy and ...
Our data reveal a cell-autonomous deficit in spindle positioning that disturbs the asymmetric localization of cortical NUMA-dynein motor complexes in BRCA1 ...
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Aurora-A Kinase as a Promising Therapeutic Target in Cancer
Linking cell cycle to asymmetric division: Aurora-A phosphorylates the Par complex to regulate Numb localization. Cell (2008) 135(1):161–73. doi:10.1016/j ...
[57]
Cell non-autonomy amplifies disruption of neurulation by mosaic ...
Feb 19, 2021 · Here we report that neural fold elevation during mouse spinal neurulation is vulnerable to deletion of the VANGL planar cell polarity protein 2 (Vangl2) gene.Missing: symmetric | Show results with:symmetric
[58]
Aging and aging-related diseases: from molecular mechanisms to ...
Dec 16, 2022 · Hutchinson-Gilford progeria syndrome: a premature aging disease caused by LMNA gene mutations. Ageing Res. Rev. 33, 18–29 (2017). Article ...
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Loss of symmetric cell division of apical neural progenitors drives ...
Aug 22, 2024 · Increased asymmetric cell division of apical neural progenitors during early development reduces the number of progenitors available for ...
[60]
Abnormal Early Cleavage Events Predict Early Embryo Demise
Oct 13, 2014 · Human embryos resulting from abnormal early cleavage can result in aneuploidy and failure to develop normally to the blastocyst stage.
[61]
Association of early cleavage, morula compaction and blastocysts ...
Jan 7, 2024 · Cleavage pattern predicts developmental potential of day 3 human embryos produced by IVF. ... Time lapse imaging of embryos is useful in in ...