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Biofouling

Biofouling is the unwanted accumulation and growth of microorganisms, algae, plants, and animals on submerged artificial surfaces in aquatic environments, encompassing both microfouling by bacteria and biofilms and macrofouling by larger sessile organisms such as barnacles, mussels, and bryozoans.^[1]^[2] This natural ecological process initiates with the adsorption of dissolved organic molecules forming a conditioning film, followed by bacterial adhesion and biofilm development, which serves as a scaffold for subsequent colonization by diatoms, protozoans, and multicellular organisms.^[3] The progression typically occurs in stages influenced by environmental factors like water temperature, salinity, nutrient availability, and flow velocity, with biofilms providing protection and nutrients that facilitate community assembly.^[4] In marine and industrial contexts, biofouling imposes substantial operational challenges by increasing frictional drag on ship hulls—potentially elevating fuel consumption by up to 40%—clogging intake systems and heat exchangers, and accelerating corrosion through microbially influenced mechanisms.^[5]^[6] These effects translate to significant economic burdens, including heightened maintenance costs and reduced efficiency across shipping, aquaculture, and desalination industries, where biofouling can account for 5-10% of production expenses in aquaculture alone.^[7] Moreover, biofouling vectors non-indigenous species dispersal via vessel hulls and structures, exacerbating biodiversity loss and ecosystem disruption through competitive exclusion and habitat alteration.^[8] Mitigation strategies have evolved from highly toxic organotin-based paints, phased out due to persistent environmental contamination and bioaccumulation in non-target organisms, toward physical methods like ultrasonic treatments, mechanical cleaning, and biomimetic non-toxic coatings inspired by natural antifouling defenses such as shark skin textures or enzyme-releasing surfaces.^[3]^[9] Despite advances, persistent challenges remain in balancing efficacy against ecological impacts, with ongoing research emphasizing sustainable, low-leach alternatives to curb both performance degradation and secondary pollution.^[10]

Definition and Biological Mechanisms

Core Processes of Adhesion and Growth

The initial stage of biofouling adhesion involves the rapid formation of a conditioning film on submerged surfaces, primarily through the adsorption of dissolved organic macromolecules such as proteins and humic substances from the surrounding aqueous environment. This process occurs within minutes to hours of immersion, driven by hydrophobic interactions, van der Waals forces, and electrostatic attractions between the molecules and the substrate.^[11] The conditioning layer alters the surface's physicochemical properties, providing a nutrient-rich and chemically modified interface that facilitates subsequent microbial colonization, with protein adsorption densities varying based on surface hydrophobicity and charge.^[12] Bacterial adhesion follows, marking the onset of primary fouling, where planktonic bacteria reversibly attach to the conditioned surface via weak physical forces before transitioning to irreversible binding through specific adhesins, pili, and flagella-mediated interactions. In marine settings, this attachment is influenced by substratum surface energy and chemistry, with hydrophobic bacteria preferring similarly hydrophobic surfaces for stronger initial contact, often quantified by reduced attachment on hydrophilic or zwitterionic-modified materials.^[13] Adhesion strength is further modulated by bacterial motility and extracellular appendages, enabling cells to overcome shear forces in dynamic flows, as observed in studies of marine Pseudomonas and Vibrio species.^[14] Once attached, bacterial growth initiates biofilm development through cell division, aggregation into microcolonies, and production of extracellular polymeric substances (EPS), forming a matrix that embeds cells and promotes three-dimensional expansion. This maturation phase involves quorum sensing for coordinated EPS synthesis—primarily polysaccharides, proteins, and DNA—enhancing structural integrity and resistance to detachment, with biofilm thickness reaching micrometers to millimeters over days to weeks depending on nutrient availability and environmental conditions.^[15] Growth dynamics exhibit exponential phases driven by metabolic activity, transitioning to plateau as resource limitations and EPS accumulation stabilize the community, setting the stage for secondary colonizers like diatoms and protozoans.^[16]

Stages of Biofilm and Community Development

The process of biofilm and community development in biofouling begins with the rapid formation of a conditioning film, where dissolved organic molecules and inorganic ions adsorb onto submerged surfaces within seconds to minutes, modifying surface chemistry and promoting microbial adhesion.^[17] This initial layer creates a favorable substrate for primary colonizers.^[17] Bacterial attachment follows, starting with reversible adhesion of planktonic cells via weak van der Waals forces, electrostatic interactions, and hydrophobic effects, often mediated by flagella or pili.^[18] This progresses to irreversible attachment, where bacteria produce adhesins and extracellular polymeric substances (EPS), including polysaccharides and extracellular DNA, forming stable microcolonies and initiating the EPS matrix that embeds cells and enhances resistance to shear forces.^[18] Quorum sensing regulates this transition, coordinating gene expression for EPS synthesis and aggregation.^[18] Biofilm maturation occurs in two phases: first, proliferation into multilayered microcolonies with increased EPS production, followed by development of a three-dimensional architecture featuring water channels for nutrient diffusion and waste removal.^[18] These structures confer persistence on surfaces like ship hulls and pipes, with bacteria exhibiting heightened tolerance to antimicrobials and environmental stresses.^[18] Dispersion concludes the cycle, as cells detach via enzymatic EPS degradation or mechanical forces, seeding new colonization sites.^[18] In marine biofouling, the bacterial biofilm underpins community succession, attracting secondary microfoulers such as diatoms and other microalgae that integrate into the EPS matrix, forming a slime layer that alters hydrodynamics and provides habitat.^[17] This microfouling stage facilitates settlement of macrofouling organisms, including macroalgae and invertebrate larvae (e.g., barnacles, mussels), which attach using adhesive secretions and develop into complex, multi-species assemblages over weeks to months.^[17] Interspecies interactions, including competition and facilitation, drive this progression toward climax communities dominated by hard-shelled invertebrates.^[17] The overall succession is probabilistic, influenced by environmental factors like water flow, nutrient availability, and temperature, with early bacterial dominance shaping later diversity.^[17]

Organisms and Ecosystems

Microorganisms and Initial Fouling

The initial phase of biofouling is dominated by microorganisms, particularly bacteria, which establish biofilms on submerged surfaces following the adsorption of a conditioning film of dissolved organic matter (DOM). This conditioning film, formed through rapid sorption of macromolecules like proteins and humic substances via van der Waals forces and hydrophobic interactions, modifies surface hydrophobicity and charge, promoting subsequent microbial adhesion within minutes to hours of exposure.^[19]^[20] In marine environments, this layer selectively adsorbs to substrates such as plastics and metals, influencing the composition of early colonizers.^[20] Bacteria serve as primary colonizers, attaching reversibly through weak interactions before transitioning to irreversible adhesion via adhesins and pili, leading to microcolony formation encased in extracellular polymeric substances (EPS). Biofilm maturation involves quorum sensing-mediated gene expression for EPS production, nutrient acquisition, and resistance to shear forces, with key species including Pseudoalteromonas, Vibrio, and Pseudomonas in marine settings.^[21]^[22] This bacterial biofilm alters surface topography and chemistry, signaling secondary colonizers like diatoms through chemical cues such as autoinducers.^[23] Diatoms, unicellular algae, contribute to initial fouling by adhering via mucilage pads and stalks, often within days of bacterial establishment, forming dense layers that increase surface roughness and hydrodynamic drag. Interactions between bacteria and diatoms, including quorum sensing effects, enhance biofilm stability and thickness, with diatoms dominating under nutrient-rich conditions.^[24]^[23] Protozoa and fungi may also participate early, grazing on bacteria or adding to EPS, but bacteria and diatoms typically comprise the bulk of the initial microbial community, setting the stage for macrofouling.^[24]^[25]

Macroorganisms and Complex Communities

Macroorganisms in biofouling, often termed macrofouling, encompass larger eukaryotic species such as invertebrates and macroalgae that settle on submerged surfaces following initial microbial colonization. These include hard-fouling organisms with calcareous exoskeletons or shells, like acorn barnacles (Balanus spp.), mussels (Mytilus spp.), oysters, and serpulid polychaete tubeworms, which attach permanently using adhesive mechanisms such as cement glands or byssal threads. Soft-fouling macroorganisms comprise macroalgae (e.g., kelps and seaweeds), tunicates (ascidians), bryozoans, hydroids, sponges, and anemones, lacking rigid calcareous structures but forming dense mats or encrustations.^[26]^[3]^[27] These macroorganisms contribute to complex communities through ecological succession, where larval or spore settlement is facilitated by underlying biofilms that provide cues for attachment and initial stability. Early macro-colonizers, such as algae or hydroids, create heterogeneous substrates that enable secondary settlement by larger species, leading to stratified assemblages with vertical zonation influenced by depth, light, and hydrodynamics. For instance, barnacle epibionts on mussels can increase susceptibility to algal fouling, with prevalence of Porphyra sp. rising from 3-7% on clean mussels to 32-40% on those with barnacles, demonstrating facilitative interactions within communities. Community structure is further shaped by biotic factors like competition for space and resources, as well as abiotic variables including shear stress, which modulates biomass accumulation and species composition by dislodging weaker attachments.^[28]^[29]^[30] In marine environments, these communities exhibit dynamic metabolic interactions and resilience, with multispecies assemblages showing greater tolerance to environmental stressors like temperature fluctuations compared to monocultures. Hydrodynamic forces, such as water flow, play a critical role in dictating community development, favoring robust attachers like barnacles in high-flow areas while permitting softer forms in sheltered zones. Such complexity underscores the progression from simple microbial films to biodiverse ecosystems, amplifying biofouling impacts on artificial structures.^[25]^[31]^[32]

Impacts

Economic Costs and Efficiency Losses

Biofouling significantly elevates operational expenses in maritime transport by augmenting hydrodynamic drag on vessel hulls, which necessitates increased fuel consumption to maintain speed and propulsion efficiency. Studies indicate that even light biofouling can reduce vessel efficiency by 10-16%, while severe macrofouling may diminish cruising speed by up to 86%, compelling operators to burn substantially more fuel.^[33] In the global shipping sector, valued at $14 trillion, this drag effect translates to fuel consumption increases of 5-15% in shaft power and overall usage, with barnacle accumulation alone capable of exacerbating emissions and operational costs fleet-wide.^[34]^[35]^[36] In power generation and desalination facilities, biofouling clogs intake systems and heat exchangers, reducing thermal efficiency and elevating energy demands for cooling and water production. For the U.S. energy sector, annual biofouling-related costs at power plants reach approximately $50 billion, primarily from maintenance, downtime, and efficiency losses.^[37] In reverse osmosis desalination plants, fouling accounts for about 24% of operational expenditures, driven by heightened energy use, frequent cleaning, and membrane replacements.^[38] Globally, earlier assessments pegged power sector management costs at up to $15 billion annually as of 2008, underscoring persistent challenges despite mitigation efforts.^[39] Aquaculture operations face direct production cost hikes of 5-10% attributable to biofouling on nets, cages, and infrastructure, encompassing labor-intensive cleaning, structural repairs, and yield reductions from obstructed water flow.^[40] Broader invasive macrofouling bivalves have incurred cumulative global damages exceeding $63.7 billion (in 2017 USD) from 1980 onward across sectors, including freshwater ecosystems where they impair infrastructure and fisheries.^[41] The International Maritime Organization further classifies these collective impacts as falling within a billion-dollar annual range, highlighting biofouling's role in compounding fuel, maintenance, and regulatory compliance burdens.^[42]

Environmental and Ecological Consequences

Biofouling serves as a primary vector for the global spread of invasive aquatic species, enabling non-indigenous organisms to colonize new ecosystems via attachment to ship hulls, offshore structures, and recreational vessels. This translocation has facilitated the introduction of over 50% of known non-indigenous aquatic species in many regions, leading to profound ecological disruptions including biodiversity loss and altered trophic dynamics.^[43]^[44] Invasive species introduced through biofouling often outcompete native organisms for resources such as food, space, and light, resulting in population declines of indigenous species and shifts in community structure. For instance, biofouling tube worms like Hydroides elegans have invaded coastal habitats, where they modify substrata, reduce available settlement sites for larvae, and promote further invasions by creating suitable conditions for additional non-natives. Such ecosystem engineering alters habitat complexity and nutrient cycling, with cascading effects on benthic communities and primary productivity.^[45] A prominent example is the zebra mussel (Dreissena polymorpha), which spread across North American waterways following its initial introduction to the Great Lakes in 1988, exacerbating invasions through biofouling on vessels and infrastructure. These mussels filter large volumes of water—up to 1 liter per individual per day—depleting phytoplankton and increasing water clarity, which in turn reduces benthic algae and disrupts food webs supporting native fish populations. Native unionid mussels have experienced up to 90% declines in some areas due to zebra mussel overgrowth, leading to local extinctions and simplified bivalve assemblages.^[46]^[47]^[48] Beyond invasions, biofouling communities can enhance local disease transmission and pathogen persistence in aquatic environments, particularly in aquaculture settings where fouled nets and structures harbor parasites and bacteria harmful to both farmed and wild stocks. In freshwater systems, biofoulers like bryozoans and sponges accumulate on dams and pipes, altering flow regimes and oxygen levels, which indirectly affect migratory fish and riparian ecosystems. These changes underscore biofouling's role in reducing ecosystem resilience to other stressors, such as climate variability.^[49]^[45]

Operational and Safety Effects

Biofouling on marine vessel hulls substantially increases hydrodynamic drag, elevating power requirements and compromising propulsion efficiency. Studies indicate that even light slime layers can reduce fuel efficiency by 10-16%, while heavy macrofouling may diminish maximum cruising speeds by up to 86%, necessitating higher engine outputs to maintain service speeds.^[33] In internal seawater systems, accumulation leads to pipe occlusion and heightened frictional losses, restricting flows critical for cooling, ballasting, and sanitation, thereby extending transit times and demanding unscheduled interventions.^[50] ^[51] Industrial operations face analogous disruptions, particularly in heat exchangers and desalination facilities where biofouling diminishes thermal transfer rates and membrane permeability. For instance, in reverse osmosis systems, fouling layers exacerbate pressure differentials, curtailing throughput and accelerating component wear, often requiring intensified cleaning cycles that interrupt production.^[52] In power generation and offshore platforms, unchecked growth impedes coolant circulation, risking thermal overloads and reduced system reliability under load.^[53] Safety implications arise from biofouling-induced failures in essential infrastructure. Vessel internal systems fouling can impair emergency seawater intakes for fire suppression or engine cooling, heightening vulnerability to onboard hazards during voyages.^[51] Moreover, biofouling facilitates the transoceanic dispersal of viable non-indigenous species, posing biosecurity threats that undermine ecosystem stability and indirectly amplify navigational risks through proliferated invasive proliferations altering habitats.^[44] ^[54] In water treatment contexts, persistent blockages elevate rupture probabilities in pressurized conduits, endangering personnel and contiguous structures.^[52]

Detection and Monitoring

Traditional and Emerging Techniques

Traditional techniques for biofouling detection rely on indirect performance metrics and manual sampling. In membrane filtration systems, monitoring increases in transmembrane pressure or declines in permeate flux serves as a primary indicator, with pressure differentials often rising by 20-50% before significant operational impacts occur, though these methods cannot differentiate biofouling from inorganic scaling or particulate fouling.^[55] ^[56] Visual inspections, including direct observation of surface accumulation via diving or removal for scrutiny, remain standard for ship hulls and submerged structures, supplemented historically by olfactory detection of decay odors from organic matter.^[57] Laboratory analyses of scraped or swabbed samples, such as dry mass weighing to quantify biomass per unit area or colony-forming unit (CFU) plating to estimate viable microbial counts (e.g., 10^6-10^8 CFU/cm² in early stages), provide compositional insights but require destructive sampling and delay results by days.^[58] Light microscopy and scanning electron microscopy (SEM) visualize biofilm morphology post-staining, revealing layered structures but limited to ex situ evaluation with resolutions down to 1-10 µm.^[58] Emerging techniques prioritize non-destructive, real-time sensing for early intervention. Electrochemical impedance spectroscopy (EIS) measures impedance shifts from biofilm-induced conductivity changes, detecting onset within hours via electrode arrays in flow systems, with sensitivity to biomass increases as low as 10 µg/cm².^[59] ^[60] Optical coherence tomography (OCT) employs near-infrared interferometry for cross-sectional imaging of biofilm thickness (up to 1-2 mm) in situ, achieving 5-10 µm resolution without contact, as validated in membrane modules since 2017.^[58] Ultrasonic time-domain reflectometry (UTDR) uses acoustic wave reflections to track deposition rates at 1-5 µm precision over time, enabling continuous profiling in pipes and heat exchangers.^[58] Mechanical methods like quartz crystal microbalance (QCM) quantify mass accumulation through frequency dissipation shifts (e.g., 1 Hz corresponding to ~17 ng/cm²), integrated into sensors for lab-to-field transitions.^[59] For marine applications, remotely operated vehicles (ROVs) with high-resolution cameras and automated image analysis via machine learning classify fouling severity from pixel-based features, reducing subjectivity in hull assessments.^[61] ^[62] These sensor-driven approaches, often combined in multiparametric systems, address traditional limitations by providing quantifiable, predictive data for predictive maintenance.^[59]

Challenges in Early Detection

The initial stages of biofouling involve rapid attachment of microorganisms, such as bacteria and diatoms, forming thin biofilms that are typically less than 10 micrometers thick and invisible to the naked eye, necessitating specialized instrumentation for detection.^[63] These early biofilms develop within hours to days in marine environments, but their low biomass and transparency complicate visual or optical identification without invasive sampling.^[24] Non-destructive detection methods, such as fluorescence-based sensors or imaging, face limitations in sensitivity and specificity during early colonization, often failing to distinguish biofouling signals from natural environmental variability like tidal fluctuations or water chemistry changes.^[64] ^[65] In submerged applications like ship hulls or tidal turbines, dynamic underwater conditions—including currents, biofouling patchiness, and organism diversity—further hinder consistent early quantification, as sensors may misinterpret noise as fouling or vice versa.^[66] ^[67] For membrane systems in water treatment, direct early inspection is impeded by high-pressure operations and enclosed module designs, delaying detection until performance metrics like flux decline indicate advanced fouling, by which point remediation costs escalate.^[52] Early warning systems remain rare across industries, with most monitoring relying on indirect indicators of process inefficiency rather than proactive microbial assays, exacerbating economic losses from unchecked progression.^[68] Standardization gaps in detection protocols also persist, as marine ecosystem uncertainties lead to unpredictable fouling onset influenced by factors like temperature and nutrient availability.^[69]^[70]

Prevention and Control Methods

Physical and Mechanical Approaches

Physical and mechanical approaches to biofouling control utilize direct physical forces, mechanical actions, or non-chemical energy fields to inhibit organism attachment, disrupt biofilms, or remove established fouling, minimizing environmental release of biocides. These methods leverage principles such as shear stress, cavitation, or vibration to target the physical integrity of fouling layers, often proving effective for initial prevention or periodic maintenance in marine, industrial, and sensor applications. Unlike chemical strategies, they avoid persistent toxins but may require frequent application and can generate waste particulates or propagules.^[71]^[3] Mechanical cleaning employs tools like rotating brushes, scrapers, or high-pressure water jets to physically dislodge fouling from surfaces such as ship hulls, pipes, and heat exchangers. In marine contexts, in-water vessel hull cleaning has been shown to restore surface smoothness, reducing hydrodynamic drag by 5-10% and improving fuel efficiency by up to 4% in cases of slime-dominated fouling. Devices such as remotely operated vehicles (ROVs) with abrasive pads or groomers enable proactive cleaning intervals every 3-6 months, extending coating life on vessels with hard epoxy antifouling paints. However, efficacy diminishes against hard-shelled macrofouling like barnacles, and incomplete removal can accelerate recolonization if residues provide nucleation sites.^[72]^[73]^[74] Ultrasonic methods generate high-frequency sound waves (typically 20-50 kHz) that induce acoustic cavitation—microbubble collapse producing shear forces and microjets—to prevent microbial adhesion and erode early biofilms without surface contact. Commercial systems installed on ship hulls, sea chests, and propellers have reduced biofouling coverage by 70-90% over 12-24 months in field trials, particularly against diatoms and algal spores, by disrupting the extracellular polymeric substances (EPS) matrix. Power requirements range from 10-100 W per transducer, with arrays covering large areas via plate or bolt-on emitters, and no measurable impact on non-target marine life beyond the cavitation zone. Limitations include reduced performance in high-turbulence waters or against mature macrofouling, necessitating hybrid use with mechanical grooming.^[75]^[76]^[77] Other physical techniques, such as thermal treatments or electromagnetic pulses, apply localized heat (above 50°C) or fields to denature proteins in fouling cells, achieving 90-99% biofilm reduction in lab-scale pipe systems. For sensors and instrumentation, mechanical wipers coupled with physical guards (e.g., copper-nickel screens) prevent obstruction, maintaining accuracy in water quality monitoring for periods up to 6 months in fouled environments. These approaches prioritize site-specific deployment, with overall success tied to fouling stage—most effective pre-mature attachment—and integration with monitoring to optimize energy use and minimize ecological disturbance from dislodged biomass.^[71]^[78]^[79]

Chemical Antifouling Strategies

Chemical antifouling strategies rely on the incorporation of biocidal agents into surface coatings, such as paints, to release toxic compounds that prevent the settlement, growth, or survival of fouling organisms like barnacles, algae, and bacteria. These biocides create a repellent zone around the substrate by interfering with biological processes, including enzyme activity, cell division, and metamorphosis in larvae.^[80] Common formulations include solvent-based or water-dispersible paints applied to marine vessels, aquaculture nets, and industrial infrastructure, with release rates controlled to balance efficacy and durability, typically lasting 1–5 years depending on environmental conditions like water salinity and temperature.^[81] Organotin compounds, particularly tributyltin (TBT), dominated from the 1970s to the early 2000s due to their broad-spectrum toxicity and long-lasting effects, preventing fouling on ship hulls for up to 5–10 years per application. TBT's self-polishing copolymer paints, introduced in the 1970s, hydrolyzed in seawater to maintain a smooth surface while steadily leaching the biocide at rates of 0.1–0.4 μg/cm²/day, minimizing drag increases from fouling. However, TBT accumulated in sediments and caused imposex in gastropods—abnormal penile growth in females—at concentrations as low as 1–10 ng/L, prompting restrictions starting in the European Union in 1989 for small vessels and culminating in the International Maritime Organization's (IMO) Anti-Fouling System (AFS) Convention of 2001, which banned TBT on ships globally effective September 2008.^[82]^[80] Copper-based biocides, primarily cuprous oxide (Cu₂O), now constitute over 90% of commercial antifouling paints, leveraging copper's toxicity to algae, invertebrates, and microbes via disruption of metabolic enzymes and oxidative stress at concentrations of 10–50 μg/L. These are often augmented with "booster" biocides—organic compounds like dichlofluanid (2–5% by weight), zinc pyrithione, or Irgarol 1051—to target resistant species such as tunicates or diatoms, achieving up to 80–95% fouling reduction in field trials over 12–24 months. Paint types include ablative (eroding layer releasing 5–25 μg Cu/cm²/day), controlled-depleting polymer (porous matrix for steady release), and self-polishing variants that maintain low roughness coefficients (k < 10⁻⁵) to reduce fuel consumption by 4–6%.^[83]^[84]^[85] Despite efficacy, copper leaching often exceeds the minimum inhibitory concentration (around 5–10 μg/L for sensitive species), contributing to chronic toxicity in coastal ecosystems, including reduced biodiversity in sediments and bioaccumulation in shellfish at levels up to 100 mg/kg dry weight. Regulations vary: the U.S. EPA monitors copper under the Clean Water Act, with some states like Washington proposing release limits of <9 μg/cm²/month by 2028, while the EU's REACH framework restricts boosters like diuron since 2008 due to groundwater persistence (half-life >100 days). Emerging hybrid strategies incorporate quaternary ammonium compounds or silanes for synergistic effects, but peer-reviewed assessments indicate persistent challenges with biocide resistance in fouling communities and unintended ecological trade-offs.^[84]^[83]^[85]

Biological and Non-Toxic Innovations

Biological control strategies employ living organisms or their derivatives to target biofouling communities without introducing persistent toxins. Bacteriophages, viruses that infect and lyse specific bacteria, have demonstrated potential in disrupting biofilm formation on surfaces like water treatment membranes by selectively reducing dominant fouling species such as Pseudomonas and Bacillus.^[52] In laboratory assays, phage cocktails applied to fouled membranes reduced bacterial densities by up to 90% within 24 hours, though field efficacy remains limited by environmental factors like salinity and temperature variability.^[3] Predatory bacteria, such as Bdellovibrio-like organisms, prey on biofilm-embedded cells, offering another targeted approach; studies in 2021 showed they decreased Escherichia coli biofilms by 70-80% in static marine simulations.^[86] Enzymatic treatments represent a key non-toxic innovation, using hydrolases to degrade extracellular polymeric substances (EPS) that anchor biofilms. Proteases and glycosidases, immobilized on surfaces or released periodically, cleave adhesive proteins and polysaccharides; for instance, a 2023 review highlighted subtilisin-based coatings preventing Ulva spore settlement by 85% in lab tests, with stability enhanced via encapsulation in silica matrices.^[87] These methods avoid broad-spectrum killing, preserving non-target marine life, but enzyme denaturation in saline conditions necessitates ongoing stabilization research, as half-lives often drop below 100 days in seawater.^[88] Multi-enzyme systems mimicking natural defenses, such as haloperoxidases from algae, generate localized oxidants without leaching, reducing barnacle adhesion by 60-75% in immersion trials conducted in 2024.^[89] Quorum sensing inhibitors (QSIs) disrupt bacterial cell-to-cell signaling, halting EPS production and biofilm maturation at early stages. Natural QSIs derived from marine algae or bacteria, like furanones, inhibit Acyl-homoserine lactone pathways, with 2021 studies reporting 50-70% reductions in Vibrio biofilms on silicone surfaces without cytotoxicity to higher organisms.^[90] Grafted onto membranes, QSIs extended operational flux in reverse osmosis systems by 20-30% before fouling onset, per nanofiber-embedded trials in 2024.^[91] Challenges include specificity, as QS pathways vary across species, requiring cocktails for broad efficacy.^[92] Biomimetic surfaces emulate natural low-fouling structures, such as shark skin denticles or lotus leaf microstructures, to minimize adhesion via topography and low surface energy. Riblet-patterned polydimethylsiloxane (PDMS) coatings reduced Ulva zoospores by 80% and barnacle attachment by 90% in dynamic flow tests, as documented in 2020 field exposures.^[93] Superhydrophobic nano-textures, inspired by pitcher plants, promote self-cleaning through Cassie-Baxter states, with 2023 innovations achieving <5% protein adsorption in protein-rich seawater analogs.^[94] Fouling-release silicone hybrids, non-toxic by design, rely on weak boundary layers for easy detachment at shear forces above 10 Pa, outperforming traditional paints in vessel trials by maintaining hull speeds with 15% less drag.^[95] Durability persists 2-5 years, though microplastic shedding raises minor ecological questions under scrutiny.^[96] Integrated approaches combining QSIs with biomimetic textures show synergistic effects; a 2024 study on hybrid nanofiltration membranes reported 95% fouling mitigation over 30 days, versus 60% for single methods.^[97] These innovations prioritize ecological compatibility, with life-cycle assessments indicating 70-90% lower environmental impact than copper-based alternatives, though commercialization lags due to scalability and cost—enzymatic coatings average $50-100/m² versus $20/m² for legacy options.^[98] Ongoing trials emphasize hybrid systems for versatile applications in shipping, aquaculture, and desalination.^[99]

Regulatory and Management Frameworks

International Standards and Guidelines

The International Maritime Organization (IMO) provides the primary international guidelines for managing biofouling on ships to prevent the transfer of invasive aquatic species. Adopted via Resolution MEPC.207(62) on July 15, 2011, these voluntary guidelines recommend strategies including the development of ship-specific biofouling management plans, maintenance of biofouling record books, and optimization of anti-fouling systems during design, construction, and operation.^[100] They apply to all ships, with emphasis on vessels over 400 gross tonnage engaged in international voyages, and promote practices such as regular in-water inspections and cleaning to minimize fouling accumulation.^[44] These 2011 guidelines were revised and updated through Resolution MEPC.378(80) on July 7, 2023, introducing a more risk-based approach tailored to individual ships' operational profiles, including enhanced focus on niche areas prone to fouling like rudders and propellers.^[101] The 2023 version maintains non-mandatory status but encourages integration with the IMO's 2004 Ballast Water Management Convention and the 2001 Anti-Fouling Systems (AFS) Convention, which prohibits organotin compounds like tributyltin (TBT) on ships since its entry into force on September 17, 2008, to reduce environmental harm from biocides while addressing biofouling risks.^[44] Key recommendations include documenting anti-fouling system applications, monitoring fouling levels through visual assessments or diver surveys, and ensuring compliance via port state control inspections. Complementary international efforts include guidelines for in-water cleaning systems, such as the 2022 "Guidelines for Testing Ship Biofouling In-Water Cleaning Systems" developed under the IMO's auspices, which standardize efficacy testing to verify reduced resuspension of biofouling organisms and compliance with environmental thresholds.^[102] These frameworks prioritize empirical risk assessments over uniform mandates, reflecting evidence that biofouling transfer varies by voyage patterns, water temperatures, and vessel types, though implementation remains inconsistent due to reliance on voluntary adoption.^[103] No binding international standards exist beyond the AFS Convention's prohibitions, with ongoing IMO discussions as of 2025 considering potential mandatory requirements to align with global invasive species prevention goals.^[104]

National and Regional Regulations

Australia enforces stringent biofouling management requirements for vessels arriving in its ports to prevent the introduction of invasive marine species, as outlined by the Department of Agriculture, Fisheries and Forestry (DAFF). Effective from July 1, 2024, international vessels must submit a pre-arrival biofouling report and comply with one of three options: maintaining an approved biofouling management plan with regular inspections and cleaning; ensuring all biofouling is removed from the hull, internal systems, and equipment within 30 days prior to arrival; or arriving with a demonstrably clean hull verified by haul-out inspection.^[105] Non-compliance can result in vessel detention, cleaning orders, or fines under the Biosecurity Act 2015.^[106] New Zealand mandates biofouling management under the Craft Risk Management Standard (CRMS) for all arriving vessels, updated in October 2023, to minimize biosecurity risks from hull and niche area fouling.^[107] Vessels must provide evidence of compliance via a Biosecurity Pre-Arrival Report, including records of maintenance such as dry-docking, antifouling application, and in-water cleaning, with hulls required to be clean or managed to low-risk levels upon entry.^[108] From May 1, 2025, submission of this report became mandatory, enforced by the Ministry for Primary Industries under the Biosecurity Act 1993, with penalties for inadequate management including quarantine or treatment at the operator's expense.^[109] In the United States, federal regulations primarily address biofouling through the U.S. Coast Guard (USCG) requirements under 33 CFR 151.2050, mandating removal of fouling organisms from hulls, piping, and tanks for vessels equipped with ballast water management systems, though broader management plans are recommended for compliance with international guidelines.^[110] Regionally, California imposes specific biofouling controls under Title 2, California Code of Regulations, Article 4.8, requiring vessels over 300 gross tons to implement management plans, including haul-out cleanings and reporting, effective for existing vessels after their first out-of-water maintenance post-January 1, 2018, to protect coastal waters from nonindigenous species.^[111] Similar state-level measures exist in other Pacific states, focusing on invasive species prevention rather than uniform national mandates.^[112] The European Union regulates antifouling systems primarily through implementation of the International Convention on the Control of Harmful Anti-fouling Systems (AFS Convention), prohibiting organotin compounds like tributyltin (TBT) on ships since 2008 to mitigate environmental harm, with enforcement via EU Regulation (EC) No 782/2003.^[113] Additional biocide restrictions under the Biocidal Products Regulation (EU) No 528/2012 govern active substances in paints, with stricter purchase and authorization rules effective from 2025 to limit marine pollution, though biofouling management for invasives remains guided by voluntary national plans rather than binding regional hull-cleaning mandates.^[114] These measures prioritize chemical controls over comprehensive biofouling removal, differing from biosecurity-focused approaches in Australia and New Zealand.^[115]

Historical Development

Ancient and Pre-Modern Observations

Early observations of biofouling date to classical antiquity, where fouling was recognized as a drag-inducing accumulation on ship hulls. In the fourth century BC, Aristotle attributed the slowing of vessels to small marine organisms adhering to hulls, which he described as resembling fish but likely referring to barnacles or similar encrustations.^[116] Plutarch, in the first century AD, similarly noted that weeds and ooze fouling hulls reduced speed, recommending scraping as a remedial action to restore performance.^[117] Pliny the Elder, in his Natural History (circa 77 AD), documented instances of marine creatures like the remora (Echeneis) and murex attaching to hulls and halting ships under sail, interpreting these as direct causes of impeded motion rather than mere encumbrances.^[117] ^[116] These accounts reflect an empirical awareness of biofouling's hydrodynamic penalties, though causal mechanisms were not dissected scientifically; instead, fouling was often mythologized alongside practical necessities for long voyages by Phoenicians and Egyptians as early as 1000 BC, where large fleets implied recurring maintenance against hull degradation.^[117] In the pre-modern era, medieval and Renaissance mariners expanded on these insights through experiential records. Viking shipbuilders in the tenth century applied seal tar to hulls, acknowledging worm and growth penetration as threats to wooden integrity.^[117] By the thirteenth to fifteenth centuries, European fleets, including those of Venice and Aragon, routinely used pitch, tar, and tallow mixtures, with English records from Henry VI's reign (1421–1471) describing lead sheathing on keels to counter barnacles and teredo worms, derived from Spanish practices.^[117] Columbus-era expeditions (late fifteenth century) required frequent careening to remove barnacle and algal layers, underscoring fouling's role in velocity loss and structural damage during transoceanic travel.^[117] These observations, preserved in naval logs and treatises, prioritized empirical mitigation over theoretical analysis, highlighting biofouling's persistent challenge to pre-industrial navigation.^[117]

Modern Scientific Research and Milestones

Scientific investigations into biofouling mechanisms gained momentum in the early 20th century, with researchers identifying microbial biofilms as critical initiators of fouling on submerged surfaces, a recognition dating back approximately 80-90 years prior to the 2010s.^[118]^[119] Early studies emphasized the role of bacterial attachment and slime formation in conditioning surfaces for subsequent algal and macroorganism settlement, shifting focus from purely mechanical removal to biological processes. By the mid-20th century, experimental work at marine laboratories, such as those examining diatom and protozoan contributions, established the sequential fouling model: adsorption of organic molecules forming a conditioning film, followed by bacterial colonization, microalgal growth, and invertebrate larvae attachment.^[118] The late 20th century marked milestones in elucidating biochemical and ecological interactions, including the 1989 analysis by Wahl of epibiosis dynamics, which outlined how surface properties influence initial microbial adhesion and community succession in marine environments.^[120] In 1992, Clare and colleagues pioneered molecular strategies for non-toxic antifouling by targeting settlement cues, laying groundwork for biomimetic interventions.^[120] These efforts coincided with growing evidence of biofilms' regulatory functions, culminating in the 2007 finding by Qian et al. that marine biofilms actively mediate macrofouler recruitment through chemical signaling.^[120] Into the 21st century, research advanced toward genetic and microbial ecology mechanisms, with 2006 demonstrations that certain marine bacteria produce inhibitory metabolites disrupting fouling cascades.^[120] A 2020 study by Liang et al. identified bacterial flagellar genes as regulators of biofilm matrix production and mussel larval settlement, highlighting prokaryotic motility's role in host-microbe interactions.^[120] Concurrently, metagenomic sequencing revealed biofilm-associated viruses influencing adhesion and polysaccharide degradation, distinct from free-living marine viromes.^[17] Recent discoveries include bacterial extracellular vesicles inducing invertebrate metamorphosis, as seen in Pseudoalteromonas luteoviolacea effects on Hydroides elegans, underscoring phage-like structures in fouling progression.^[17] These findings, while advancing causal models of biofouling, expose persistent gaps in multi-species dynamics and biogeographic variability beyond temperate zones.^[17]

Controversies and Debates

Efficacy of Biocides Versus Environmental Concerns

Biocides, including copper compounds and organic boosters such as Irgarol 1051 and diuron, provide robust short-term control of biofouling by releasing toxic ions or compounds that inhibit larval settlement and microbial adhesion on surfaces like ship hulls and aquaculture nets.^[121] Copper-based antifouling paints, which dominate commercial applications, achieve low effective concentrations (EC50 values often below 10 μg/L for barnacle cyprids) through continuous leaching, outperforming non-biocidal alternatives in high-fouling environments during initial deployment periods of 6-12 months.^[122] Booster biocides like Sea-Nine 211 (DCOIT) enhance copper's efficacy by targeting specific fouling stages, with field trials demonstrating up to 90% reduction in biomass accumulation compared to untreated controls in subtropical waters.^[123] However, efficacy wanes over time due to biocide depletion and adaptation by fouling communities, necessitating frequent reapplication and contributing to inconsistent long-term performance.^[121] Environmental concerns arise primarily from the non-selective toxicity and persistence of these biocides, which accumulate in sediments and harm non-target marine organisms. Copper leaching from paints often exceeds effective antifouling doses, reaching concentrations of 5-50 μg/L in harbors, where it impairs photosynthesis in algae (EC50 ~2-10 μg/L for species like Skeletonema costatum) and induces oxidative stress in shellfish, reducing populations by 20-50% in chronically exposed areas.^[84] Booster biocides exacerbate risks; Irgarol 1051, a triazine herbicide, inhibits photosystem II in autotrophs at LC50 values of 0.1-1 μg/L for marine algae and seagrasses, persisting in ecosystems with half-lives of 100-200 days and bioaccumulating in mollusks.^[124] Diuron similarly yields acute toxicities (24-h LC50 ~10-50 μg/L) to copepods and fish larvae, disrupting food webs at the base through chronic exposure levels detected in coastal sediments up to 1 μg/g dry weight.^[125] Antifouling paint particles (APPs) from eroded copper coatings prove particularly toxic, causing 50-100% mortality in infaunal species like cockles (Cerastoderma edule) at environmentally relevant doses.^[126] Debates center on balancing proven biocidal performance against ecological trade-offs, with empirical data indicating that while biocides minimize operational costs (e.g., reducing ship fuel use by 5-10% via drag reduction), their widespread application correlates with biodiversity declines in marinas and bays, prompting restrictions like the 2008 global TBT ban and ongoing copper limits in regions such as the EU and U.S. states.^[127] Proponents argue that incomplete alternatives, such as silicone foul-release coatings, underperform in static conditions (fouling coverage >20% after 6 months vs. <5% for biocides), potentially increasing greenhouse gas emissions from higher fuel consumption, yet critics highlight systemic underestimation of cumulative biocide loads, as peer-reviewed models predict sediment burdens doubling without intervention.^[128] Regulatory frameworks increasingly favor hybrid approaches, but first-principles assessments reveal that biocide efficacy stems from broad-spectrum lethality, inherently conflicting with selective environmental protection absent site-specific monitoring.^[129]

Trade-Offs in Regulation and Innovation

The phase-out of tributyltin (TBT)-based antifouling paints, mandated globally under the International Maritime Organization's (IMO) Anti-Fouling Systems Convention effective January 1, 2008, exemplified a core regulatory trade-off by prioritizing environmental protection over proven efficacy, prompting a surge in alternative technologies but at the expense of increased fuel consumption and operational costs for vessels. TBT's self-polishing copolymer formulations had achieved fouling control with minimal drag penalties, but its persistence in sediments caused widespread imposex in gastropods and ecosystem disruption, justifying the ban based on empirical toxicity data from field studies. Post-ban, the industry shifted to copper-based self-polishing paints and silicone foul-release coatings, which spurred innovation in binder chemistries and biocide alternatives, yet these often underperform in static conditions, leading to biofouling accumulation that raises hull resistance by up to 40% and fuel use by 20-40% over a vessel's dry-docking cycle. This efficacy gap has been quantified in lifecycle assessments showing net increases in greenhouse gas emissions from higher propulsion demands, highlighting how stringent biocide restrictions can inadvertently exacerbate the very environmental issues they aim to mitigate through indirect emissions pathways.^[130]^[131]^[132] Regulatory frameworks continue to impose trade-offs by favoring precautionary approaches that delay innovation in emerging non-toxic methods, such as enzyme-based or biomimetic coatings, due to protracted approval processes under conventions like the IMO's 2011 Biofouling Guidelines—recently updated toward mandatory status via MEPC.323(74) in 2019 and further refined at MEPC 83 in 2025—which require vessel management plans but lack harmonized standards for novel technologies, creating compliance uncertainties that deter investment. Peer-reviewed analyses indicate that while these guidelines reduce invasive species spread risks, they fragment global enforcement, with regional variations (e.g., stricter biocide limits in the EU under REACH) raising R&D costs by mandating extensive environmental fate testing, often exceeding $10 million per formulation, and slowing market entry for disruptive innovations like protein-repellent surfaces or in-water robotic cleaning systems. Empirical data from post-TBT transitions reveal that such hurdles have sustained reliance on legacy copper biocides, whose runoff contributes to coastal metal accumulation, underscoring a causal tension where over-regulation stifles adaptive technologies that could achieve fouling control with lower ecological footprints.^[133]^[134]^[135] Balancing these dynamics requires weighing short-term environmental safeguards against long-term innovation incentives, as evidenced by economic models projecting that optimized biofouling management could cut global shipping emissions by 10-15% through integrated hull cleaning and coating advancements, yet current regulatory silos—prioritizing endpoint toxicity over holistic lifecycle impacts—hinder such synergies. For instance, in-water hull cleaning, promoted as a low-emission alternative to dry-docking, faces biosecurity restrictions due to fragment release risks, with studies showing potential for 10-100-fold increases in propagule dispersal under suboptimal protocols, forcing operators into costlier, less frequent interventions. This has prompted calls for performance-based regulations that incentivize verifiable reductions in fouling-induced drag, potentially accelerating biotech innovations like quorum-sensing disruptors, but implementation lags amid institutional biases toward risk-averse biocide curbs rather than empirical validation of net benefits. Ultimately, unresolved trade-offs manifest in higher compliance burdens—41% of operators reporting penalties or port denials tied to biofouling in recent surveys—underscoring the need for causal-realist frameworks that prioritize data-driven thresholds over blanket prohibitions to foster resilient antifouling paradigms.^[136]^[135]

References

[1]
Biofouling - an overview | ScienceDirect Topics
Biofouling refers to the detrimental deposition of biological growth (by both micro- and macroorganisms) with other constituents of natural waters.
[2]
Biofouling - an overview | ScienceDirect Topics
Biofouling can be defined as the unwanted growth of organisms on artificial surfaces submerged in freshwater or saltwater environments. Biofouling development ...
[3]
[PDF] Biofouling and Mitigation Methods: A Review
Apr 2, 2022 · It is defined as the accumulation and growth of marine organisms on surfaces immersed in the sea/non-sterile environment [15]. Biofouling ...
[4]
Advances in biofouling mitigation: A review - Taylor & Francis Online
Feb 21, 2016 · 1. Biofouling can be defined as the accumulation of very small organisms (microorganisms) such as bacteria, fungi, and algae on surfaces.
[5]
Assessment of the economic, environmental and safety impact of ...
Oct 1, 2023 · Biofouling development on ships leads to substantial increases in power requirement, fuel consumption and Green House Gas (GHG) emissions, ...Missing: industry | Show results with:industry
[6]
Fueling Economic Benefits with Remote Removal of Marine Biofouling
According to the United States Office of Naval Research, by increasing water resistance, biofouling can boost a ship's fuel consumption by as much as 40 percent ...
[7]
The impact and control of biofouling in marine aquaculture: a review
Consequently, the economic costs associated with biofouling control are substantial. Conservative estimates are consistently between 5-10% of production ...
[8]
[PDF] MARINE BIOFOULING: NON-INDIGENOUS SPECIES AND ...
The biofouling of submerged anthropogenic surfaces and factors that contribute to the spread of non-indigenous species (NIS) have both received substantial ...
[9]
(PDF) Review article: Biofouling: Lessons from nature - ResearchGate
Aug 6, 2025 · Biofouling is generally undesirable for many applications. An overview of the medical, marine and industrial fields susceptible to fouling is presented.
[10]
Marine biofouling and the role of biocidal coatings in balancing ...
Therein fouled hulls cause increased fuel consumption, greenhouse gas emissions, and the spread of invasive aquatic species.
[11]
Perceiving and Countering Marine Biofouling: Structure, Forces, and ...
Mar 20, 2025 · JournalsPeer-reviewed chemistry research; SubjectsSpecific research ... For control and utilization of bacterial adhesion and biofilms, adhesion ...
[12]
Controlling Experimental Parameters to Improve Characterization of ...
Uncontrolled protein adsorption and cell binding to biomaterial surfaces may lead to degradation, implant failure, infection, and deleterious inflammatory ...
[13]
Effect of Substratum Surface Chemistry and Surface Energy on ...
Upon submersion in a nonsterile aqueous liquid, most surfaces become rapidly colonized by collections of bacteria and other microorganisms. These attached cells ...
[14]
Dynamics of marine bacterial biofouling communities after initial ...
Biofouling is caused by living organisms, including bacteria, diatoms, fungi, protozoans, macroalgae and/or invertebrates, that are attached to submerged ...
[15]
Biofilm Development | Microbiology Spectrum - ASM Journals
Biofilm development includes initial attachment, microcolony formation, maturation, and dispersal. It can occur through multiple pathways, and is species- ...
[16]
expanding the conceptual model of biofilm formation - PubMed Central
Apr 1, 2023 · We suggest a revised conceptual model that encompasses the three major steps of biofilm life, aggregation, growth and disaggregation ...
[17]
Understanding biofouling and contaminant accretion on submerged ...
Jun 21, 2023 · This review identifies knowledge gaps in the understanding of marine fouling and highlights approaches to better direct development of effective anti-fouling ...
[18]
Understanding bacterial biofilms: From definition to treatment ...
The maturation of bacterial biofilms is the fourth stage of biofilm development. Bacterial biofilms mature as bacteria replicate and multiply in the EPS matrix ...
[19]
Understanding the Fundamental Basis for Biofilm Formation on ...
Jun 24, 2021 · ... attachment of microorganisms. Hence, CFs are a biologically ... conditioning film formation in the marine environment. Microbiology ...
[20]
Conditioning Film and Early Biofilm Succession on Plastic Surfaces
Aug 2, 2021 · In this study, we tested the hypothesis that the first conditioning film of dissolved organic matter (DOM) sorbs selectively to polymer substrates.
[21]
Mechanisms and Impact of Biofilms and Targeting of Biofilms Using ...
The development of biofilm involves four main stages, starting with cellular attachment, to formation of microcolonies, then biofilm maturation, and finally ...
[22]
Biofilm formation: mechanistic insights and therapeutic targets - PMC
Dec 15, 2023 · In this review, with Pseudomonas as the primary subject of study, we review and discuss the mechanisms of bacterial biofilm formation and ...
[23]
Revealing the Bacterial Quorum-Sensing Effect on the Biofilm ... - NIH
Jul 20, 2023 · Bacteria and diatoms are the main components of fouling biofilms, and their interactions are paramount in biofilm communities and the biofouling ...
[24]
Marine Biofilms: A Successful Microbial Strategy With Economic ...
Bacteria and diatoms have been found to dominate marine biofilms but the ratio bacteria:diatoms:flagellates observed in biofilms in the White Sea was 640:4 ...Abstract · Introduction · Marine Biofilms Protect... · Preventing Biofouling and...
[25]
Directed assembly of biofilm communities for marine biofouling ...
Aug 25, 2025 · Biofilm formation in the ocean involves adhesion of different organisms, including bacteria, fungi, diatoms, protozoans, invertebrate larvae, ...
[26]
Marine Biofouling: A European Database for the Marine Renewable ...
2.1. Macrofouling comprises calcareous hard-fouling organisms such as acorn barnacles, mussels and tubeworms and soft-fouling organisms such as non-calcareous ...
[27]
Marine Biofouling - an overview | ScienceDirect Topics
Marine biofouling is defined as the aggregation of fouling organisms, such as bacteria, algae, and barnacles, on submerged substrates.
[28]
Monthly Succession of Biofouling Communities and Corresponding ...
Feb 10, 2022 · Our study aimed to compare the monthly succession of the biofouling community developed at two locations each in the north- (Kuwait) and south-west (Oman) of ...
[29]
Increased algal fouling on mussels with barnacle epibionts
Mussels with barnacle epibionts showed a higher prevalence of Porphyra sp. fouling (32–40% depending on sampling date) than mussels without them (3–7%).Missing: barnacles algae
[30]
Shear stress controls prokaryotic and eukaryotic biofilm communities ...
Dec 18, 2024 · Shear stress appeared to be the primary factor influencing biomass, EPS production and community structure and composition.
[31]
Impact of hydrodynamics on community structure and metabolic ...
In marine environments, biofouling refers to the complex colonization of submerged substrates by micro- and macro-organisms (Railkin, 2003). This biological ...
[32]
Experimental insights on biofouling growth in marine renewable ...
Marine biofouling, i.e. the growth of microorganisms such as bacteria and microalgae (microfouling) and macroorganisms such as barnacles, mussels and ...
[33]
How biofouling impacts vessel efficiency — and how to fix it
Mar 8, 2021 · Even minor microfouling can decrease vessel efficiency by 10 - 16%; macrofouling and result in loss of 86% of a vessel's cruising speed.Missing: consumption | Show results with:consumption
[34]
Biofouling & Invasive Species - Silverstrand Capital Insights
Nov 14, 2024 · Biofouling creates a cost burden for the $14 trillion global shipping industry. Presence of these organisms increase drag, and reduce fuel ...
[35]
Estimating the effect of biofouling on ship shaft power based on ...
The efficiency lost due to biofouling can be 5–15% of the total shaft power and fuel usage (Molland et al. Citation2014; Wang and Lutsey Citation2013; Hakim et ...
[36]
New study confirms barnacle biofouling is a big burden ... - Selektope
Apr 25, 2025 · High levels of barnacle biofouling on underwater hulls across the global shipping fleet can significantly drag down decarbonisation efforts and increase fuel ...Missing: efficiency | Show results with:efficiency
[37]
PNNL Scientists Keep Biofouling at Bay | Feature
Mar 11, 2019 · For the energy sector in the United States, the estimated cost of biofouling at power plants is about $50 billion per year, primarily for ...
[38]
Cost of fouling in full-scale reverse osmosis and nanofiltration ...
Mar 15, 2021 · We found that the cost of fouling in the RO plants was around 24% of OPEX, while the fouling related costs in NF cases was only around 11% due to the low ...
[39]
A 15 month-monitoring of biofouling in relationship with hydrological ...
Mar 15, 2025 · In 2008, a global study estimated up to $15 billion in costs related to managing and preventing marine biofouling for one year in the power ...Missing: economic | Show results with:economic
[40]
Biofouling in marine aquaculture: a review of recent research and ...
The direct economic costs of managing biofouling in the aquaculture industry are estimated to be 5–10% of production costs (Lane and Willemsen Citation2004).
[41]
Economic costs of invasive bivalves in freshwater ecosystems
Mar 8, 2022 · Cumulative total global costs of invasive macrofouling bivalves were $ 63.7 billion (2017 US$) across all regions and socio-economic sectors between 1980 and ...
[42]
Coatings Tricks to Prevent Biofouling
The International Maritime Organization estimates that annual biofouling costs lie in the billion-dollar range. Economic losses are not the only negative impact ...
[43]
Biofouling and IAS - GloFouling Partnerships
Considering that in excess of 50% of non-indigenous species may have been transported via biofouling, the global impacts invasive aquatic species transferred ...
[44]
Biofouling - International Maritime Organization
Biofouling on ships entering the waters of States may result in the establishment of invasive aquatic species which may pose threats to human, animal and plant ...Missing: consequences | Show results with:consequences
[45]
Invasion and ecological impact of the biofouling tube worm ...
Invasive species can outcompete native species for resources such as food, habitat, and light, which can lead to declines in the populations of native species ( ...Missing: consequences | Show results with:consequences
[46]
Invasive Zebra Mussels (U.S. National Park Service)
Apr 2, 2021 · The zebra mussels also outcompete the natives for food and space, and because of their fast reproduction can quickly overwhelm a water system.
[47]
zebra mussel (Dreissena polymorpha) - Species Profile
Zebra mussels can have profound effects on the ecosystems they invade. They primarily consume phytoplankton, but other suspended material is filtered from the ...
[48]
The Great Lakes' most unwanted: Characterizing the impacts of the ...
The invasion of zebra and quagga mussels was associated with high impacts, as realized by increased water clarity and a decline in phytoplankton biomass and ...
[49]
Biofouling animals in fresh water: biology, impacts, and ecosystem ...
Mar 3, 2014 · Biofoulers accumulate on underwater surfaces and adversely affect human activities and infrastructure. Freshwater biofoulers are much less ...Missing: consequences | Show results with:consequences
[50]
Biofouling occlusion of ships' internal seawater systems - PubMed
Jun 27, 2023 · Biofouling of ships' internal seawater systems (ISS) can cause significant operational issues and is a potential transfer mechanism for marine ...Missing: effects | Show results with:effects
[51]
[PDF] A Review of Biofouling of Ships' Internal Seawater Systems - Frontiers
Oct 28, 2021 · The impact of biofouling on ISS and vessel operations is based on increased surface roughness of pipework and equipment, restricted water flow, ...
[52]
Biofouling of Water Treatment Membranes - PubMed Central - NIH
It is difficult to control, and significant economic resources have been dedicated to the development of effective biofouling monitoring and control strategies.
[53]
Biofouling dynamic and its impact on ship powering and dry-docking
Feb 1, 2022 · The biofouling development significantly impacts the ship's performance, powering demands, fuel consumption, and overall shipping costs.
[54]
Biosecurity risks associated with in-water and shore-based marine ...
Biofouling removed from vessels during cleaning therefore poses a biosecurity risk as it may contain viable, non-indigenous organisms or their reproductive ...
[55]
Mechanisms, diagnosis, and monitoring of biofouling in membrane ...
This paper mainly reviews the progress in the research and development of biofouling reduction in membrane processes.
[56]
Biofouling in membrane processes - ALVIM Srl
Commonly, the development of a fouling layer is detected indirectly by measuring the loss in permeate flux or the increase in trans membrane pressure.
[57]
Biofouling in reverse osmosis: phenomena, monitoring, controlling ...
Oct 27, 2016 · Techniques by which this is done can range from primitive inspection through sight or smell, sampling and lab testing to more advanced ...
[58]
A Technological Understanding of Biofilm Detection Techniques - NIH
Biofouling is a sequential, four-step phenomenon governed by several physical, chemical, and biological factors: attachment, proliferation, maturation, and ...
[59]
Real-time biofilm detection techniques: advances and applications
This review provides a focused examination of advancements in real-time biofilm detection techniques, specifically in electrochemical, optical and mechanical ...
[60]
[PDF] Mechanisms, diagnosis, and monitoring of biofouling in membrane ...
Among spectroscopic techniques, EIS is a prom- ising technique for “in-line” and “early-stage” biofouling detection and monitoring techniques with relatively ...
[61]
Automating the assessment of biofouling in images using expert ...
Feb 2, 2021 · Here we apply deep learning to automate the classification of images from in-water inspections to identify the presence and severity of fouling.Missing: monitoring | Show results with:monitoring
[62]
Technology Portal : IMO Test Biofouling
Underwater robots or ROVs equipped with cameras can be used to inspect and make a preliminary assessment of biofouling.
[63]
Microbial Surface Colonization and Biofilm Development in Marine ...
In this review, recent progress in the study of marine microbial surface colonization and biofilm development is synthesized and discussed.
[64]
Fault detection for salinity sensors in the Columbia estuary
Mar 19, 2003 · [5] Early biofouling detection is made difficult by the normal variability of salinity measurements. Tides cause the measurements to vary ...
[65]
Early non-destructive biofouling detection and spatial distribution
The primary objective of this study was to evaluate whether biofouling could be detected and quantified with the optode at an early stage, before a significant ...
[66]
Tidal Stream Turbine Biofouling Detection and Estimation - MDPI
Apr 24, 2023 · This paper proposes a review-based roadmap investigating the use of data-driven techniques, more specifically machine learning-based approaches, to detect and ...
[67]
Biofouling detection and classification in Tidal Stream Turbines ...
Detecting biofouling presents challenges due to its unpredictable occurrence and the uncertainties associated with the diverse and dynamic marine ecosystem ...
[68]
(PDF) Microbial Biofouling: Unsolved Problems, Insufficient ...
Biofouling is detected by its effect on process performance or product quality and quantity. Early warning systems are very rare, although they could save ...
[69]
Destructive and non-destructive methods for biofouling analysis ...
Jun 15, 2012 · The current study investigated the use of novel methods for assessment of biofouling potential, using process stream samples obtained from the ...
[70]
A critical review of marine biofilms on metallic materials - Nature
Mar 24, 2022 · Metal substrates in marine environments impose unique challenges that ultimately shape the community and physical structure of biofilms. A ...
[71]
Physical Approaches to Prevent and Treat Bacterial Biofilm - PMC
Dec 29, 2022 · Special attention has been paid to physical strategies such as heat, light, sound, and electromagnetic energy, and their uses in biofilm ...
[72]
Submerged surfaces exposed to marine biofouling
Physical removal of biofouling by underwater cleaning methods is efficient and cost-effective so mechanical cleaning is usually considered for long-term ...
[73]
[PDF] Biofouling of vessels - a challenge to the ship industry and the ...
Finally, epoxy antifouling coatings provide a hard surface which enables regular mechanical cleaning (Lewis, 2020; Tamburri et al., 2020). Figure 1.
[74]
[PDF] In-water vessel cleaning: current and emerging technologies ...
Mechanical cleaning may also increase the susceptibility of surfaces to settlement. (Floerl et al. 2005) if surfaces are not sterilized or repainted after ...
[75]
Ways to control harmful biofilms: Prevention, inhibition, and ...
Many physical methods, such as treatment with ultrasound and magnetic fields, can be applied to effectively eradicate biofilms (Figure 3D). Ultrasonic ...
[76]
What is ultrasonic antifouling? The future of biofouling management
Oct 26, 2024 · Ultrasonic antifouling is an eco-friendly, non-biocidal approach to preventing and managing marine growth (or biofouling) on a ship's hulls.
[77]
Ultrasonic antifouling: 4 cutting edge options put to the test
Aug 16, 2022 · An electronic device mounted to your boat's hull produces sound waves that discourage fouling from establishing a hold.
[78]
https://www.ysi.com/File%2520Library/Documents/Specification%2520Sheets/E108-YSI-Antifouling-Tips-Book.pdf
[79]
[PDF] Biofouling protection for marine environmental sensors - OS
If the sensor is hardly fouled mechanical methods such as high pressure water jet or brushes or chemical methods are some- times used to clean up the ...<|separator|>
[80]
Antifouling strategies: History and regulation, ecological impacts and ...
The most commonly used biocides in antifouling paints are Tributyltin (TBT), Chlorothalonil, Dichlofluanid, Sea-Nine 211, Diuron, Irgarol 1051 and Zinc ...
[81]
Antifouling paints - Coastal Wiki
Sep 27, 2024 · Tributyltin paint was widely used in the 1970s and 1980s as a highly efficient biocide in antifouling paints for ship and boat hulls. TBT was ...
[82]
Anti-fouling systems - International Maritime Organization
One of the most effective biocides for use in anti-fouling paints, developed in the 1960s, was the organotin compound tributyltin (TBT), which has been proven ...
[83]
Use of Copper-Based Antifouling Paint: A U.S. Regulatory Update
The efficacy of the active biocide ingredients used in antifouling bottom paint to contain and impede the growth of biofouling on boat hulls is of global ...
[84]
Antifouling paints leach copper in excess – study of metal release ...
Nov 1, 2020 · These findings suggest that current antifouling paints on the market are leaching copper in excess of the effective dose in brackish and marine waters.
[85]
[PDF] Antifouling Paints in Washington State: Third Report to the Legislature
Jun 1, 2024 · Most coatings contain an inorganic copper-based primary biocide, such as cuprous oxide, together with one or more organic booster biocides or co ...
[86]
Biological-based control strategies for MBR membrane biofouling
Apr 29, 2021 · The membrane biofouling control methods mainly include physical (e.g. membrane relaxation and backflushing, etc.), chemical (e.g. NaClO, NaOH ...Abstract · INTRODUCTION · FORMATION AND IMPACT... · MBR MEMBRANE...
[87]
Application of enzymes for targeted removal of biofilm and fouling ...
This article provides a summary of the research that has been done to date on the ability of enzymes to prevent biofouling. An overview of using antifouling ...
[88]
Enzyme-based antifouling coatings: a review - PubMed
A systematic overview is presented of the literature that reports the antifouling (AF) protection of underwater structures via the action of enzymes.Missing: prevention | Show results with:prevention
[89]
Biomimetic haloperoxidases for antifouling on the surface of marine ...
Biomimetic haloperoxidases, based on marine algae, prevent microorganism attachment. They are stable, low cost, and include vanadium, cerium, and molybdenum- ...
[90]
Use of Quorum Sensing Inhibition Strategies to Control Microfouling
Interfering with the quorum sensing bacterial communication systems has been proposed as a promising strategy to control bacterial biofilm formation, ...
[91]
Quorum Quenching Nanofibers for Anti-Biofouling Applications - MDPI
Jan 4, 2024 · Researchers are exploring the use of quorum-sensing inhibitors as a novel strategy to disrupt the communication signals that orchestrate biofilm ...Quorum Quenching Nanofibers... · 3. Results And Discussions · 3.4. Nanofiltration...
[92]
Application of quorum sensing inhibitors for improving anti ...
Jan 15, 2021 · Quorum sensing inhibitors (QSIs) have shown promising results in reducing biofouling in various membrane systems.
[93]
Recent Progress of Biomimetic Antifouling Surfaces in Marine
Aug 23, 2020 · By studying these cases, biomimetic approaches may well provide us new insights into designing and developing nontoxic antifouling paints.
[94]
Recent Developments in Biomimetic Antifouling Materials: A Review
Natural surfaces exist that are self-cleaning and/or antifouling—some have the ability to chemically inhibit settlement and growth of colonising organisms on ...
[95]
Non-toxic evolution: Advances in multifunctional antifouling coatings
This review first presents four major antifouling strategies: traditional coatings based on wettability, traditional coatings with protective structures, ...
[96]
Marine biofouling and antifouling: a decade of systematic review on ...
Oct 9, 2025 · Biofouling significantly contributes to increased drag on vessels, accelerates structural degradation, and reduces the operational efficiency of ...
[97]
Antifouling applications and fabrications of biomimetic micro ...
A critical review of biomimetic micro-structured surfaces for antifouling and other applications is provided.
[98]
Strategies for Biofouling Control: A Review from an Environmental ...
Another significant negative impact of biofouling is the alteration of local biological communities, potentially leading to competition for space and resources ...
[99]
Biofouling dynamics and antifouling innovations: Transitioning from ...
Mar 15, 2025 · These inventions seek to reduce economic consequences on marine industry by lowering device performance difficulties and maintenance expenses.<|control11|><|separator|>
[100]
[PDF] Resolution MEPC.207(62) - International Maritime Organization
Jul 15, 2011 · Biofouling Record Book Form. 2011 Guidelines for the control and management of ships' biofouling to minimize the transfer of invasive aquatic ...
[101]
[PDF] RESOLUTION MEPC.378(80) (adopted on 7 July 2023) 2023 ...
Jul 7, 2023 · The aim of the Guidelines was to provide a globally consistent approach to managing biofouling by providing useful recommendations of general ...
[102]
[PDF] Guidelines for Testing Ship Biofouling In-Water Cleaning Systems
Oct 26, 2022 · The International Maritime Organization (IMO) defines antifouling systems (AFS) as a coating, paint, surface treatment, surface, or device that ...<|separator|>
[103]
Understanding the IMO's new guidance on biofouling
Feb 23, 2024 · The revised International Maritime Organization (IMO) Biofouling Guidelines came into force in 2023 and provide updated guidance on how to prevent the spread ...
[104]
The IMO and Biofouling in 2025: What to expect - Evac
Mar 5, 2025 · Regular inspections are critical for biofouling control. IMO's guidelines suggest systematic checks of hulls, niches, and other submerged ...
[105]
Australian biofouling requirements - DAFF
Jul 25, 2024 · Australian biofouling requirements include a pre-arrival report, and vessels must use one of three options: a management plan, cleaning within ...
[106]
Biofouling in Australia - DAFF
Dec 5, 2023 · Vessel biofouling and ship ballast water can introduce exotic marine species into Australian waters. Once here, they can spread and threaten ...
[107]
Biofouling management | NZ Government
Oct 8, 2025 · All vessels must provide evidence of biofouling management before they arrive in New Zealand. The Craft Risk Management Standard (CRMS) for ...
[108]
Commercial vessel biofouling requirements | NZ Government
Oct 8, 2025 · As of the 13 October 2023, all biosecurity requirements for the topside of vessels and for biofouling are in the updated Craft Risk Management ...October 2023: Changes To The... · Vessel Biofouling... · Maintain, Inspect And Clean...
[109]
New Zealand mandates Biosecurity Pre-Arrival Report from 1 May ...
Biosecurity New Zealand has issued a reminder regarding the use of the updated mandatory Biosecurity Pre-Arrival Report document from 1 May 2025.New Zealand Mandates... · Biosecurity New Zealand Has... · 19 June 2025
[110]
[PDF] guidance notes on - biofouling management plans august 2019
Aug 1, 2019 · The regional requirements described in this section at the time of publishing include specific requirements by the United States of America and ...
[111]
[PDF] Article 4.8 Biofouling Management Regulations: Overview of the ...
When are they required? ◦ Existing vessels: After first regularly scheduled out-of- water maintenance on or after January 1, 2018.
[112]
[PDF] Biofouling in the U.S. Pacific States and British Columbia
Apr 11, 2017 · The topical focus of this white paper is marine biofouling as a mechanism for the transport, introduction, and spread of nonindigenous ...<|separator|>
[113]
Protecting the sea and the food chain from the effects of organotin ...
Aug 25, 2015 · It aims to prohibit organotin compounds on all ships entering EU ports in order to reduce or eliminate the adverse effects of these products.
[114]
Buying anti-fouling paint made more difficult: new biocide ... - Sea Help
Nov 9, 2024 · From 2025, stricter rules will apply to anti-fouling purchases. The new biocide regulation governs the purchase and protects the environment ...
[115]
Protecting the Marine Environment - Anti-fouling - EMSA
Anti-fouling paints may contain biocides (chemical substances or microorganisms) that are harmful to the marine environment.
[116]
Marine anti-fouling technologies
Mar 4, 2014 · In the fourth century BC, Aristotle credited small fish (barnacles) with the ability to slow down ships [1]. Saroyan [2] suggested that fouling ...
[117]
The History of the Prevention of Fouling* - July 1952 Vol. 78/7/593
The earliest mention of fouling that we have found is a casual reference to it in connection with the Echeneis or Remora; the fabled “ship-stopper.” This ...
[118]
A short history of microbial biofilms and biofilm infections - PubMed
Biofilms were observed by Leeuwenhoek and Pasteur, and in biofouling 80-90 years ago. The term was introduced in 1985, and their medical importance is now ...
[119]
personal history of research on microbial biofilms ... - Oxford Academic
In environmental and technical microbiology, biofilms were already shown 80–90 years ago to be important for biofouling on submerged surfaces, e.g. ships. The ...
[120]
Biofouling and Antifouling: Interactions between Microbes and ... - NIH
Mar 31, 2023 · The biofouling process refers to the undesirable accumulation of micro- and macro-organisms on manufactured surfaces.<|separator|>
[121]
Assessment of the effectiveness of antifouling solutions for ...
This study assessed the effectiveness of different antifouling strategies in a manipulative experiment by testing two common coating typologies.
[122]
Antifouling activity of commercial biocides vs. natural and natural ...
Nine biocides including commercial, natural and natural-derived products were tested. The commercial antifoulants, TBTO and Sea Nine showed low EC 50 but high ...
[123]
Evaluation of the Anti-fouling Efficacy of Bacillus licheniformis ...
Aug 12, 2021 · Anti-fouling paint formulations are often composed of inorganic biocides (typically cuprous oxide) and one or more organic or organo ...
[124]
Acute toxicities of five commonly used antifouling booster biocides to ...
Irgarol and diuron are photosynthetic system II (PSII) inhibitors (Jones and Kerswell, 2003), and both biocides are highly toxic to autotrophic aquatic species ...
[125]
Acute toxicities of five commonly used antifouling booster biocides to ...
This study comprehensively investigated the acute toxicities of copper, tributyltin (TBT), and five commonly used booster biocides including Irgarol, diuron, ...
[126]
Environmental concentrations of antifouling paint particles are toxic ...
Jan 1, 2021 · APPs derived from copper-based biocidal paints proved most toxic to cockles and ragworms. While exposure to silicone-based non-biocidal APPs ...<|separator|>
[127]
[PDF] 12 Booster biocide antifoulants: is history repeating itself?
Tributyltin (TBT) was widely used as an effective antifouling agent in paints for ships and boats until the European Community restricted its use in 1989 ...
[128]
Marine Coatings: Making Sense of U.S., State, and Local Mandates ...
“Biofouling control has tremendous environmental benefits that range from energy savings to slowing the spread of invasive species. Over regulating copper-based ...
[129]
Environmental and Economic Impact of an Innovative Biocide ... - NIH
Jan 12, 2023 · From an economic point of view, the application of the antifouling coating entails several expenses, such as the cost of maintenance of the boat ...
[130]
Antifouling technology—past, present and future steps towards ...
The imminent ban of environmentally harmful tributyltin (TBT)-based paint products has been the cause of a major change in the antifouling paint industry.Missing: pre- | Show results with:pre-
[131]
An absurd scenario in 2021: Banned TBT-based antifouling ...
Jan 20, 2022 · As a result of these efforts, a reduction in TBT levels and their biological effects has been observed in coastal areas worldwide (Lahbib et al.
[132]
The Biofouling Challenge: An Untapped Emissions Solution - Evac
Aug 19, 2025 · Unmanaged biofouling can raise fuel use by 20–40%. Proactive management offers immediate savings, compliance gains, and emissions ...
[133]
Past, present and (possible) future of biofouling regulatory ...
Jul 26, 2025 · This work focuses on the evolution of international marine environmental protection legislation and analyzes existing regulatory instruments ...
[134]
Sustainable Marine Coatings: Comparing the Costs, Benefits, and ...
Biocidal coatings generally performed better short-term, but biocide-free options showed comparable efficacy in some cases and clear environmental benefits.
[135]
Disruption, adaptation, and the future of antifouling industries
This paper argues that UCRs are not merely technological innovations but systemic disruptors that threaten legacy coating industries while enabling new models ...
[136]
New report reveals the consequences of poor biofouling management
Jun 4, 2025 · 41% have faced regulatory penalties due to biofouling related issues; 38% have been denied port access due to non-compliance issues related ...